CN101268785B - Bacteriostasis composition for garbage burying field and preparation thereof - Google Patents
Bacteriostasis composition for garbage burying field and preparation thereof Download PDFInfo
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- CN101268785B CN101268785B CN2007100382862A CN200710038286A CN101268785B CN 101268785 B CN101268785 B CN 101268785B CN 2007100382862 A CN2007100382862 A CN 2007100382862A CN 200710038286 A CN200710038286 A CN 200710038286A CN 101268785 B CN101268785 B CN 101268785B
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Abstract
The present invention discloses an antimicrobial compound used in a landfill waste site and a preparation method thereof. The antimicrobial compound includes the components and contents that the sum of a microzyme colony is 1 to 3*10<3>cfu/ml; the sum of a lactobacillus colony is 2 to 4*10<5>cfu/ml; the sum of a Bacillus subtilis colony is 1 to 3*10<3>cfu/ml. The antimicrobial compound used in the landfill waste site of the present invention is innoxious and uninjurious, can effectively restrain the growing of flies in garbage and the propagation of spoilage organisms, and has obvious social benefit.
Description
Technical field
The present invention relates to a kind of bacterial classification mixture, relate in particular to a kind of bacteria inhibiting composition that is used for refuse landfill and preparation method thereof.
Background technology
Along with improving constantly of people's living standard, the continuous increase of population, the generation of rubbish is also more and more, and the processing of rubbish has caused people's great attention.
The treatment process of present rubbish generally has landfill method, burning method etc., and landfill method is a kind of method comparatively commonly used, invest little, easy to operate, still, also there are many defectives, especially screwworm in the rubbish and maggot pupa, as not anticipating, will directly form secondary pollution, as the rubbish stockyard, often fly causes disaster, and has directly influenced environment all round.Simultaneously, the harmful microorganism in the rubbish also will cause the severe contamination of environment as not restraining.
Summary of the invention
The technical issues that need to address of the present invention are to disclose a kind of bacteria inhibiting composition that is used for refuse landfill, to overcome the above-mentioned defective that prior art exists.
The bacteria inhibiting composition that is used for refuse landfill of the present invention is a kind of aqueous solution, and its component and content comprise:
Yeast total number of bacterial colony 1～3 * 10
3Cfu/ml
Milk-acid bacteria total number of bacterial colony 2～4 * 10
5Cfu/ml
Subtilis total number of bacterial colony 1～3 * 10
3Cfu/ml
Preferably:
Yeast total number of bacterial colony 2 * 10
3Cfu/ml
Milk-acid bacteria total number of bacterial colony 3 * 10
5Cfu/ml
Subtilis total number of bacterial colony 2 * 10
3Cfu/ml
The preparation method who is used for the bacteria inhibiting composition of refuse landfill of the present invention comprises the steps:
(1) yeast, milk-acid bacteria and Bacillus subtilis strain are inoculated in respectively in separately the activation medium, activation culture, incubation time is 2～3 days, culture temperature is 30～40 ℃;
(2) then with the bacterial classification after each activation and the mixture of activation medium, mix, be inoculated in the mother liquor substratum, carry out the cultivation of mother liquor anaerobism under airtight, incubation time is 10～20 days, and culture temperature is 30～45 ℃;
The ratio of the combined amount of each bacterial classification is:
Yeast total number of bacterial colony: milk-acid bacteria total number of bacterial colony: subtilis total number of bacterial colony
＝1～3×10
3cfu∶2～4×10
5cfu∶1～3×10
3cfu?cfu；
The total add-on of the bacterial classification after the activation and the mixture of activation medium is 8～12% of a mother liquor substratum weight;
(3) again the mother liquor of step (2) is added in the fermention medium based on 1～5% of the gross weight of fermention medium, under airtight, carry out the anaerobically fermenting cultivation, incubation time is 30～40 days, culture temperature is 30～40 ℃, be preferably in preceding 10～12 days, stirred every day 3～4 hours, stirred 2～3 hours middle 10～12 day every day, stirred 1～2 hour 10～12 day every day the back, at last 20～30 ℃ of static cultivations down, the PH numerical value of the gas that produces in culturing process is 3.0～5.0, promptly stops to cultivate, and the culture that is obtained is the bacteria inhibiting composition that is used for refuse landfill of the present invention.
The component of saccharomycetic activation medium and parts by weight concentration are:
2～4 parts of maltose
2～4 parts of yeast extract pastes
4～6 parts of peptones
8～12 parts of glucose
80～120 parts in water
The component of the activation medium of milk-acid bacteria and parts by weight concentration are:
25～35 parts of skimmed milks
4～6 parts of yeast extract pastes
8～12 parts of glucose
80～120 parts of Tomato juice
800～1200 parts in water
The component of the activation medium of subtilis and parts by weight concentration are:
15～25 parts of sucrose
KNO
30.1～0.3 part
MgSO
47H
20.05～0.1 part of O
0.07～0.1 part of KCl
KH
2PO
40.1～0.3 part
Ca (NO
3)
20.25～0.4 part
800～1200 parts in water
The component and the parts by weight concentration of said mother liquor substratum and fermention medium are:
The close 5-8 part of sugar
1～3 part of yeast extract paste or fish sauce
Bean sprouts juice 4-6 part
The 3-5 of Tomato juice part
80～120 parts in water
The bacteria inhibiting composition that is used for refuse landfill of the present invention, can be used for suppressing the growth of the fly of rubbish, restrain the harmful microbe breeding in the rubbish, make refuse treatment can reach innoxious, lower environmental pollution, can reduce simultaneously the accumulating amount of rubbish, save land resources, concrete using method is as follows:
With the bacteria inhibiting composition that is used for refuse landfill that is obtained, dilute with water, preparation becomes the aqueous solution that content is 50～100mg/ml, with 1～2 kilogram/meter
2Consumption be sprayed on the rubbish surface, can reach the purpose that suppresses fly growth in the rubbish and suppress harmful microbe breeding in the rubbish, inhibiting rate can reach more than 80%.
Animal experiment proves, the bacteria inhibiting composition that is used for refuse landfill of the present invention is nontoxic.
By above-mentioned disclosed technical scheme as seen, bacterium fluid composition of the present invention avoids using chemicals, can effectively suppress fly growth and harmful microbe breeding in the rubbish, has very remarkable social benefit.
Embodiment
Described bacterial classification is all chosen in Chinese microbial preservation center.
Embodiment 1
(1) yeast, milk-acid bacteria and Bacillus subtilis strain are inoculated in respectively in separately the activation medium, activation culture, incubation time is 3 days, culture temperature is 35 ℃;
(2) then with the bacterial classification after each activation and the mixture of activation medium, be inoculated in after the mixing in the mother liquor substratum, carry out the cultivation of mother liquor anaerobism under airtight, incubation time is 15 days, and culture temperature is 40 ℃;
The ratio of the combined amount of each bacterial classification is:
Yeast total number of bacterial colony: milk-acid bacteria total number of bacterial colony: subtilis total number of bacterial colony
＝2×10
3cfu∶3×10
5cfu∶2×10
3cfu；
The total add-on of the bacterial classification after the activation and the mixture of activation medium is 10% of a mother liquor substratum weight;
(3) again the mother liquor of step (2) is added in the fermention medium based on 3% of the gross weight of fermention medium, under airtight, carry out the anaerobically fermenting cultivation, incubation time is 35 days, culture temperature is 35 ℃, at preceding 11 days, stirred every day 3 hours, stirred 2 hours middle 12 day every day, stirred 2 hours 12 day every day the back, at last 25 ℃ of static cultivations down, the PH numerical value of the gas that produces in culturing process is 4, promptly stops to cultivate, and the culture that is obtained is the bacteria inhibiting composition that is used for refuse landfill of the present invention.
The component of saccharomycetic activation medium and parts by weight concentration are:
3 parts of maltose
3 parts of yeast extract pastes
5 parts of peptones
10 parts of glucose
100 parts in water
The component of the activation medium of milk-acid bacteria and parts by weight concentration are:
30 parts of skimmed milks
5 parts of yeast extract pastes
10 parts of glucose
100 parts of Tomato juice
1000 parts in water
The component of the activation medium of subtilis and parts by weight concentration are:
20 parts of sucrose
KNO
30.2 part
MgSO
47H
20.05 part of O
0.07 part of KCl
KH
2PO
40.1 part
Ca (NO
3)
20.25 part
1000 parts in water
The component and the parts by weight concentration of said mother liquor substratum and fermention medium are:
Close 6 parts of sugar
2 parts of yeast extract paste or fish sauces
5 parts in bean sprouts juice
4 parts of Tomato juice
100 parts in water
Embodiment 2
Toxicity test, the animal test method that adopts GB15670-1995 to stipulate is tested the product of embodiment 1, and the result is as follows:
Male and female chmice acute per os LD
50Value is all greater than 5000mg/kg.b.wt.
Embodiment 3
Bacteriostatic test:
With the bacteria inhibiting composition that is used for refuse landfill that embodiment 1 obtains, thin up, preparation becomes the aqueous solution that content is 50mg/ml, with 1 kilogram/meter
2Consumption be sprayed on the rubbish surface, adopt (2001) amount to recognize (Soviet Union) word (z0188) standard and detect, the streptococcus aureus inhibiting rate can reach more than 81%, the fly growth inhibition ratio can reach more than 80%.
Embodiment 4
With the bacteria inhibiting composition that is used for refuse landfill that embodiment 1 obtains, thin up, preparation becomes the aqueous solution that content is 80mg/ml, with 1.5 kilograms/meter
2Consumption be sprayed on the rubbish surface, the volume of organic matter rubbish 7 days descends 30%.
Embodiment 5
With the bacteria inhibiting composition that is used for refuse landfill that embodiment 1 obtains, thin up, preparation becomes the aqueous solution that content is 1000mg/ml, with 2 kilograms/meter
3Consumption add in the sewage, descended 80% in BOD3 days, descended 80% in COD3 days.
Claims (4)
1. preparation is used for the method for the bacteria inhibiting composition of refuse landfill, it is characterized in that, comprises the steps:
(1) yeast, milk-acid bacteria and Bacillus subtilis strain are inoculated in respectively in separately the activation medium, activation culture, incubation time is 2～3 days, culture temperature is 30～40 ℃;
(2) bacterial classification after each will activate then and the mixture of activation medium are inoculated in after the mixing in the mother liquor substratum, carry out the cultivation of mother liquor anaerobism under airtight, and incubation time is 10～20 days, and culture temperature is 30～45 ℃;
(3) again the mother liquor of step (2) is added in the fermention medium based on 1～5% of the gross weight of fermention medium, under airtight, carry out the anaerobically fermenting cultivation, incubation time is 30～40 days, culture temperature is 30～40 ℃, at preceding 10～12 days, stirred every day 3～4 hours, stirred 2～3 hours middle 10～12 day every day, stirred 1～2 hour 10～12 day every day the back, 20～30 ℃ of static cultivations down, the PH numerical value of the gas that produces in culturing process is 3.0～5.0 at last, promptly stops to cultivate, the culture that is obtained is the bacteria inhibiting composition that is used for refuse landfill, and its component and content comprise: yeast total number of bacterial colony 1～3 * 10
3Cfu/ml, milk-acid bacteria total number of bacterial colony 2～4 * 10
5Cfu/ml, subtilis total number of bacterial colony 1～3 * 10
3Cfu/ml;
The component and the parts by weight concentration of said mother liquor substratum and fermention medium are:
Molasses 5-8 part, 1～3 part of yeast extract paste or fish sauce, bean sprouts juice 4-6 part, the 3-5 of Tomato juice part, 80～120 parts in water.
2. method according to claim 1 is characterized in that, the component of saccharomycetic activation medium and parts by weight concentration are:
3. method according to claim 1 is characterized in that, the component of the activation medium of milk-acid bacteria and parts by weight concentration are:
4. method according to claim 1 is characterized in that, the component of the activation medium of subtilis and parts by weight concentration are:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100382862A CN101268785B (en) | 2007-03-21 | 2007-03-21 | Bacteriostasis composition for garbage burying field and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100382862A CN101268785B (en) | 2007-03-21 | 2007-03-21 | Bacteriostasis composition for garbage burying field and preparation thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101268785A CN101268785A (en) | 2008-09-24 |
| CN101268785B true CN101268785B (en) | 2011-11-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100382862A Expired - Fee Related CN101268785B (en) | 2007-03-21 | 2007-03-21 | Bacteriostasis composition for garbage burying field and preparation thereof |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525894A (en) * | 2012-07-03 | 2014-01-22 | 天津市中敖饲料有限公司 | Preparation method and application of bacillus and lactobacillus composite antimicrobial peptides |
| CN105191989A (en) * | 2014-06-16 | 2015-12-30 | 张世峰 | Preparation method for brown sugar fermentation broth biopesticide |
| CN105112332A (en) * | 2015-08-29 | 2015-12-02 | 湖南普泰尔微科环保科技有限公司 | Culture medium for preparing microorganism deodorizing complexing agent and preparation method of culture medium |
| CN109221268A (en) * | 2018-10-24 | 2019-01-18 | 贵州欧瑞欣合环保股份有限公司 | A kind of fly eradication complexing agent and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1385384A (en) * | 2002-06-18 | 2002-12-18 | 潘金环 | Methd for industrilized organic refuse treatment and fly maggot and worm lizard cultivation |
| CN1470481A (en) * | 2002-07-23 | 2004-01-28 | 四川国策环保实业工程股份有限公司 | Municipal house refuse termenting method |
| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
| CN1850965A (en) * | 2006-05-31 | 2006-10-25 | 北京嘉博文生物科技有限公司 | Method for resource circulating treatment of dining-kitchen organic waste using compound bacteria |
| CN1861199A (en) * | 2005-05-12 | 2006-11-15 | 中国农业大学 | Biological deodorization and purification agent, and its use |
-
2007
- 2007-03-21 CN CN2007100382862A patent/CN101268785B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1385384A (en) * | 2002-06-18 | 2002-12-18 | 潘金环 | Methd for industrilized organic refuse treatment and fly maggot and worm lizard cultivation |
| CN1470481A (en) * | 2002-07-23 | 2004-01-28 | 四川国策环保实业工程股份有限公司 | Municipal house refuse termenting method |
| CN1682599A (en) * | 2004-04-14 | 2005-10-19 | 中国农业大学 | Microbial fodder additive and its preparing method and use |
| CN1861199A (en) * | 2005-05-12 | 2006-11-15 | 中国农业大学 | Biological deodorization and purification agent, and its use |
| CN1850965A (en) * | 2006-05-31 | 2006-10-25 | 北京嘉博文生物科技有限公司 | Method for resource circulating treatment of dining-kitchen organic waste using compound bacteria |
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|---|---|
| CN101268785A (en) | 2008-09-24 |
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Owner name: DAI ZHINAN Free format text: FORMER OWNER: SHANGHAI CHONGKE BUSINESS TRADING CO., LTD. Effective date: 20100409 |
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Free format text: CORRECT: ADDRESS; FROM: 200433 D160, NO.48, JINQIU ROAD, BAOSHAN DISTRICT, SHANGHAI CITY TO: 200442ROOM 101, NO.36, LANE 881, HUALING ROAD, SHANGHAI CITY |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20100409 Address after: 200442 Shanghai City Hua Ling Road No. 36 Lane 881, room 101 Applicant after: Dai Zhinan Address before: 200433 Shanghai city Baoshan District Jinqiu Road No. 48 D160 Applicant before: Shanghai Chongke Commerce and Trade Co., Ltd. |
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Granted publication date: 20111109 Termination date: 20140321 |