CN101258220A - 荧光染料化合物，共轭物及其应用 - Google Patents

荧光染料化合物，共轭物及其应用 Download PDF

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Publication number
CN101258220A
CN101258220A CNA2005800310384A CN200580031038A CN101258220A CN 101258220 A CN101258220 A CN 101258220A CN A2005800310384 A CNA2005800310384 A CN A2005800310384A CN 200580031038 A CN200580031038 A CN 200580031038A CN 101258220 A CN101258220 A CN 101258220A
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CN
China
Prior art keywords
compound
substituted
dye
nhr
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CNA2005800310384A
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English (en)
Chinese (zh)
Inventor
R·格雷厄
R·邹
K·G·尤帕德亚
S·C·本森
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Applied Biosystems Inc
Original Assignee
Applera Corp
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Filing date
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Application filed by Applera Corp filed Critical Applera Corp
Publication of CN101258220A publication Critical patent/CN101258220A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
    • C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
    • C07D219/06—Oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B15/00—Acridine dyes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1003—Carbocyclic compounds
    • C09K2211/1011—Condensed systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
    • C09K2211/10—Non-macromolecular compounds
    • C09K2211/1018—Heterocyclic compounds
    • C09K2211/1025—Heterocyclic compounds characterised by ligands
    • C09K2211/1029—Heterocyclic compounds characterised by ligands containing one nitrogen atom as the heteroatom
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrane Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Steroid Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other In-Based Heterocyclic Compounds (AREA)
CNA2005800310384A 2004-09-16 2005-09-16 荧光染料化合物，共轭物及其应用 Pending CN101258220A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61111904P 2004-09-16 2004-09-16
US60/611,119 2004-09-16

Publications (1)

Publication Number Publication Date
CN101258220A true CN101258220A (zh) 2008-09-03

Family

ID=35455743

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2005800310384A Pending CN101258220A (zh) 2004-09-16 2005-09-16 荧光染料化合物，共轭物及其应用

Country Status (8)

Country Link
US (4) US7256292B2 (enExample)
EP (2) EP2371927B1 (enExample)
JP (2) JP2008513579A (enExample)
CN (1) CN101258220A (enExample)
AU (1) AU2005287078A1 (enExample)
CA (1) CA2581174A1 (enExample)
SG (1) SG155923A1 (enExample)
WO (1) WO2006034036A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
JP2008513579A (ja) 2004-09-16 2008-05-01 アプレラ コーポレイション 蛍光染料化合物、結合体およびそれらの使用
EP2092327A2 (en) * 2006-09-18 2009-08-26 Applied Biosystems, LLC Methods, systems and apparatus for light concentrating mechanisms
US20080293127A1 (en) * 2007-05-22 2008-11-27 Applera Corporation Method to Render Pressure Sensitive Adhesive Compatible with Polymerase Chain Reaction Systems
US8652442B2 (en) 2008-11-03 2014-02-18 Washington University Bioluminescence imaging of myeloperoxidase activity in vivo, methods, compositions and apparatuses therefor
CN102232118B (zh) * 2008-12-01 2015-07-08 恰根有限公司 用于核酸检测的新型荧光染料组合物
GB201012417D0 (en) * 2010-07-23 2010-09-08 Cambridge Entpr Ltd Capilary electrophoresis of carbohydrates
EP2913408A1 (en) 2010-12-29 2015-09-02 Life Technologies Corporation Ddao compounds as fluorescent reference standards
TWI485145B (zh) 2012-10-26 2015-05-21 Ind Tech Res Inst Ｐ型有機半導體材料與光電元件
EP3802526A1 (en) 2018-05-29 2021-04-14 ELITechGroup, Inc. Carborhodamine compounds and methods of preparation thereof
US12385039B2 (en) 2020-07-02 2025-08-12 Life Technologies Corporation Trinucleotide cap analogs, preparation and uses thereof

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5118800A (en) 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4855225A (en) 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
EP0459536B1 (en) 1986-12-09 1996-09-04 Bayer Corporation Chromogenic acridinone enzyme substrates
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
US5231191A (en) 1987-12-24 1993-07-27 Applied Biosystems, Inc. Rhodamine phosphoramidite compounds
DE3912046B4 (de) * 1988-09-02 2004-03-25 Carnegie Mellon University Verfahren zum Markieren einer Komponente einer wäßrigen Flüssigkeit und lumineszenzphotostabiles Reaktionsprodukt
US5141813A (en) 1989-08-28 1992-08-25 Clontech Laboratories, Inc. Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
US5393615A (en) 1994-02-03 1995-02-28 Miles Inc. Mediators suitable for the electrochemical regeneration of NADH, NADPH or analogs thereof
ATE198511T1 (de) 1994-04-29 2001-01-15 Perkin Elmer Corp Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5728528A (en) 1995-09-20 1998-03-17 The Regents Of The University Of California Universal spacer/energy transfer dyes
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5853992A (en) 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
AU9063398A (en) 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
US6583168B1 (en) * 1997-11-25 2003-06-24 Applera Corporation Sulfonated diarylrhodamine dyes
GB2341189B (en) 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
CA2352631A1 (en) * 1998-12-14 2000-06-22 Aurora Biosciences Corporation Optical molecular sensors for cytochrome p450 activity
AU7494100A (en) 1999-09-17 2001-04-17 Amersham Pharmacia Biotech Inc. Charge-modified nucleic acid terminators
US6465644B1 (en) * 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
JP2003064083A (ja) * 2001-08-23 2003-03-05 Fuji Photo Film Co Ltd ２，３，３−トリメチル−３Ｈ−ピロロ［２，３−ｂ］ピリジン−５−スルホン酸およびその誘導体
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
JP4318296B2 (ja) * 2001-08-29 2009-08-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 標識ヌクレオシドポリホスフェート
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
WO2003020603A1 (en) * 2001-09-06 2003-03-13 Robert John Galway Collapsible packing boxes
AU2002326950A1 (en) * 2001-09-19 2003-04-01 Pe Corporation (Ny) Membrane transportable fluorescent substrates
CA2433473A1 (en) 2002-07-23 2004-01-23 F. Hoffmann-La Roche Ag Fluorescent hybridization probes with reduced background
DE60332468D1 (de) * 2002-08-29 2010-06-17 Ge Healthcare Bio Sciences Analytnachweis
CA2496462C (en) * 2002-08-29 2014-01-28 Amersham Biosciences Corp Allele specific primer extension
US6855503B2 (en) 2002-12-20 2005-02-15 Amersham Biosciences Corp Heterocyclic FRETdye cassettes for labeling biological molecules and their use in DNA sequencing
DE60335144D1 (de) 2003-02-05 2011-01-05 Ge Healthcare Bio Sciences Nukleinsäureamplifikation
JP2008513579A (ja) * 2004-09-16 2008-05-01 アプレラ コーポレイション 蛍光染料化合物、結合体およびそれらの使用
US20100227327A1 (en) 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
CN102648415A (zh) 2009-08-25 2012-08-22 生命技术公司 定量荧光蛋白标准品
EP2913408A1 (en) 2010-12-29 2015-09-02 Life Technologies Corporation Ddao compounds as fluorescent reference standards

Also Published As

Publication number Publication date
CA2581174A1 (en) 2006-03-30
US20140303003A1 (en) 2014-10-09
EP2371927A1 (en) 2011-10-05
WO2006034036A1 (en) 2006-03-30
EP1802727B1 (en) 2018-04-04
SG155923A1 (en) 2009-10-29
US20060167257A1 (en) 2006-07-27
US9745336B2 (en) 2017-08-29
US20110250699A1 (en) 2011-10-13
EP1802727A1 (en) 2007-07-04
US20090093062A1 (en) 2009-04-09
US7985602B2 (en) 2011-07-26
EP2371927B1 (en) 2020-03-11
JP2009041027A (ja) 2009-02-26
JP2008513579A (ja) 2008-05-01
US8729267B2 (en) 2014-05-20
US7256292B2 (en) 2007-08-14
AU2005287078A1 (en) 2006-03-30

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Application publication date: 20080903