DE60335144D1 - Nukleinsäureamplifikation - Google Patents

Nukleinsäureamplifikation

Info

Publication number
DE60335144D1
DE60335144D1 DE60335144T DE60335144T DE60335144D1 DE 60335144 D1 DE60335144 D1 DE 60335144D1 DE 60335144 T DE60335144 T DE 60335144T DE 60335144 T DE60335144 T DE 60335144T DE 60335144 D1 DE60335144 D1 DE 60335144D1
Authority
DE
Germany
Prior art keywords
nucleic acid
terminal
phosphate
nucleotides
detectable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335144T
Other languages
English (en)
Inventor
Anup Sood
Shiv Kumar
John Nelson
Carl Fuller
Anuradha Sekher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Global Life Sciences Solutions USA LLC
Original Assignee
GE Healthcare Bio Sciences AB
GE Healthcare Bio Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB, GE Healthcare Bio Sciences Corp filed Critical GE Healthcare Bio Sciences AB
Application granted granted Critical
Publication of DE60335144D1 publication Critical patent/DE60335144D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
DE60335144T 2003-02-05 2003-08-29 Nukleinsäureamplifikation Expired - Lifetime DE60335144D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44527403P 2003-02-05 2003-02-05
PCT/US2003/027287 WO2004072304A1 (en) 2003-02-05 2003-08-29 Nucleic acid amplification

Publications (1)

Publication Number Publication Date
DE60335144D1 true DE60335144D1 (de) 2011-01-05

Family

ID=32869330

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60335144T Expired - Lifetime DE60335144D1 (de) 2003-02-05 2003-08-29 Nukleinsäureamplifikation

Country Status (8)

Country Link
US (1) US7125671B2 (de)
EP (1) EP1590479B1 (de)
JP (1) JP2006513705A (de)
AT (1) ATE489479T1 (de)
AU (1) AU2003265857A1 (de)
DE (1) DE60335144D1 (de)
ES (1) ES2355555T3 (de)
WO (1) WO2004072304A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
WO2002004680A2 (en) * 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7727722B2 (en) * 2001-08-29 2010-06-01 General Electric Company Ligation amplification
AU2004211920B2 (en) * 2003-02-05 2009-05-14 Global Life Sciences Solutions Usa Llc Solid phase sequencing
US7256292B2 (en) 2004-09-16 2007-08-14 Applera Corporation Fluorescent dye compounds, conjugates and uses thereof
WO2007069717A1 (ja) * 2005-12-15 2007-06-21 Katsuya Honda Ｄｎａ断片を増幅するためのｄｎａの増幅方法及び鋳型ｄｎａの活性化方法
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US7563574B2 (en) * 2006-03-31 2009-07-21 Pacific Biosciences Of California, Inc. Methods, systems and compositions for monitoring enzyme activity and applications thereof
FR2906532B1 (fr) * 2006-09-28 2008-12-12 Biomerieux Sa Nouvel oligonucleotide marque
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
ES2351294B8 (es) * 2009-07-02 2012-06-06 Consejo Superior De Investigaciones Científicas (Csic) Metodo para la replicación, amplificación, o secuenciación de un adn molde.
KR101190792B1 (ko) * 2010-08-16 2012-10-12 한국과학기술연구원 변형 뉴클레오티드 및 이를 이용한 실시간 중합효소 반응
US20120171684A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3665274A1 (de) 2017-08-07 2020-06-17 DNA Script Varianten der familie-a-dna-polymerase und verwendungen davon
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10435676B2 (en) 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
EP3924500A1 (de) 2019-02-12 2021-12-22 DNA Script Effiziente produktspaltung in der template-freien enzymatischen synthese von polynukleotiden

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849478A (en) * 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
AU2180200A (en) * 1998-12-14 2000-07-03 Li-Cor Inc. A heterogeneous assay for pyrophosphate detection
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
CA2412567A1 (en) * 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
WO2002004680A2 (en) * 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1354064A2 (de) * 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use

Also Published As

Publication number Publication date
JP2006513705A (ja) 2006-04-27
US7125671B2 (en) 2006-10-24
ES2355555T3 (es) 2011-03-28
EP1590479B1 (de) 2010-11-24
EP1590479A1 (de) 2005-11-02
AU2003265857A1 (en) 2004-09-06
US20040152104A1 (en) 2004-08-05
EP1590479A4 (de) 2006-04-05
ATE489479T1 (de) 2010-12-15
WO2004072304A1 (en) 2004-08-26

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