CN101238800B - Method for marking tilapia individual - Google Patents
Method for marking tilapia individual Download PDFInfo
- Publication number
- CN101238800B CN101238800B CN2008100186679A CN200810018667A CN101238800B CN 101238800 B CN101238800 B CN 101238800B CN 2008100186679 A CN2008100186679 A CN 2008100186679A CN 200810018667 A CN200810018667 A CN 200810018667A CN 101238800 B CN101238800 B CN 101238800B
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- China
- Prior art keywords
- tilapia
- syringe needle
- mark
- tilapia mossambica
- syringe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belonging to the field of aquaculture technology relates to a labelling method of tilapia individual, particularly to injecting PIT marker into tilapia body for labelling individual. The invention is characterized in that: at first dip the marker and the injector thereof into disinfection solution; anaesthetize tilapia with anesthesia; put the disinfected marker into injector, puncture the abdominal wall from the abdominal rib side which is next to the front part of the tilapia anus with the syringe needle, push the syringe needle into abdominal cavity along abdominal wall; when the syringe needle is in the abdominal cavity, push the syringe slightly, when the marker is in the abdominal cavity of tilapia, extract the syringe needle. Labelling the tilapia individual in vivo according to the invention can improve the marker retention rate and valid identification rate, for the marker is not shed; the fish body damage degree during the labelling process is little, which does not affect normal activities of labelling fish and has very little effect on tilapia culture.
Description
Technical field
The present invention relates to a kind of method of marking tilapia individual, specifically the PIT mark is injected into and carries out individual mark in the Tilapia mossambica body, belong to technical field of aquaculture.
Background technology
In prior art, when carrying out the individual mark of Tilapia mossambica, adopt the external hanging type mark usually, promptly be hung on the Tilapia mossambica fish body with numbered label.External hanging type label commonly used has Floy mark and T phenotypic marker, and the former passes the Tilapia mossambica back by pin and line label is hung over the Tilapia mossambica body surface, and the latter passes the Tilapia mossambica afterbody with the mark rifle with mark and carries out mark.
These two kinds of external hanging type marks all need penetrate Tilapia mossambica fish body, thereby bigger to the Tilapia mossambica damage; This two kinds of marks or one section fine rule is arranged in addition; there is one section thin plastics stub to stay on the fish body; in breeding process; these fine rules or stub can twine mutually or other things in the pond on; not only can cause mark to come off; also can influence the normal activity of Tilapia mossambica even cause the damage of fish body, have a negative impact culturing the result.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, thereby a kind of method of marking tilapia individual is provided, can alleviates damage, can improve the retention rate of mark again Tilapia mossambica fish body, the effective discrimination height of mark, and minimum to the breeding process influence of Tilapia mossambica.
Main solution of the present invention is achieved in that
The method of a kind of marking tilapia individual of the present invention, adopt following processing step:
At first mark and syringe thereof were soaked in thimerosal 1～15 minute; With anaesthetic Tilapia mossambica is anaesthetized; The mark that to sterilize places in the syringe needle again, and syringe needle pierces through stomach wall from abdomen rib one side near Tilapia mossambica anus front portion, pushes intraperitoneal along stomach wall; Treat that syringe needle enters the about 0.5～1.0cm in abdominal cavity, touch injector syringe, mark is pushed the Tilapia mossambica intraperitoneal after, extract syringe needle out.
Described thimerosal is: 70～75% alcohol;
Described anaesthetic is caryophyllus oil solution, and caryophyllus oil solution percentage concentration is: 0.01%～0.02%.
Described Tilapia mossambica body weight is＞6g;
The length that described syringe needle enters the Tilapia mossambica abdominal cavity is 0.5～1.0cm.
Compared with the prior art the present invention has the following advantages:
The present invention carries out the body internal labeling of tilapia individual, can improve the retention rate of mark, and mark can not come off, effectively the discrimination height; Labeling process is less to the degree of injury of fish body, can not influence the normal activity of Tilapia mossambica, and mark is minimum to the breed influence of Tilapia mossambica.
Embodiment
Following the present invention will be further described in conjunction with the embodiments:
Embodiment one:
The method of a kind of marking tilapia individual of the present invention, adopt following processing step:
At first in 70～75% alcohol, soak more than 1 minute PIT mark and syringe thereof; Get in the 2mL caryophyllus oil adding 20L water and be mixed with anaesthetic caryophyllus oil solution, caryophyllus oil solution percentage concentration is: 0.01%～0.02%.With anaesthetic caryophyllus oil solution Tilapia mossambica is anaesthetized.The PIT mark of sterilizing is placed in the syringe needle, getting the Tilapia mossambica of the heavy 10.5g of a urosome holds in left hand, the right hand is held syringe syringe needle is pierced through stomach wall from abdomen rib one side near Tilapia mossambica anus front portion with 45 degree, after treating that syringe needle enters stomach wall, be about to syringe needle and push intraperitoneal with the direction parallel with stomach wall.Syringe needle touches injector syringe after entering the about 0.6cm in abdominal cavity, the PIT mark is pushed the Tilapia mossambica intraperitoneal after, extract syringe needle out.The described PIT mark that places in the Tilapia mossambica body that is labeled as is the Trovan ID 100A that Trovan company produces, and it is a kind of microchip of being discerned by radio frequency, outsourcing glass, be corynebacterium, size is 11.50mm * 2.12mm, and the identification frequency is 128kHz;
Embodiment two:
The method of a kind of marking tilapia individual of the present invention, adopt following processing step:
At first PIT mark and syringe thereof were soaked 5 minutes in thimerosal 70～75% alcohol; Get in the 2mL caryophyllus oil adding 20L water and be mixed with anaesthetic caryophyllus oil solution, anaesthetic caryophyllus oil solution concentration is: 0.01%.With anaesthetic caryophyllus oil solution Tilapia mossambica is anaesthetized.The PIT mark of sterilizing is placed in the syringe needle, getting the Tilapia mossambica of the heavy 40.6g of a urosome holds in left hand, the right hand is held syringe syringe needle is pierced through stomach wall from abdomen rib one side near Tilapia mossambica anus front portion with 45 degree, after treating that syringe needle enters stomach wall, be about to syringe needle and push intraperitoneal with the direction parallel with stomach wall.Syringe needle touches injector syringe after entering the about 0.9cm in abdominal cavity, the PIT mark is pushed the Tilapia mossambica intraperitoneal after, extract syringe needle out.
Embodiment three:
The method of a kind of marking tilapia individual of the present invention, adopt following processing step:
At first PIT mark and syringe thereof were soaked 10 minutes in thimerosal 70～75% alcohol; Get in the 2mL caryophyllus oil adding 10L water and be mixed with anaesthetic caryophyllus oil solution, caryophyllus oil solution anaesthetic concentration is: 0.02%.With anaesthetic caryophyllus oil solution Tilapia mossambica is anaesthetized.The PIT mark of sterilizing is placed in the syringe needle, getting the Tilapia mossambica of the heavy 60.8g of a urosome holds in left hand, the right hand is held syringe syringe needle is pierced through stomach wall from abdomen rib one side near Tilapia mossambica anus front portion with 45 degree, after treating that syringe needle enters stomach wall, be about to syringe needle and push intraperitoneal with the direction parallel with stomach wall.Syringe needle touches injector syringe after entering the about 1.0cm in abdominal cavity, the PIT mark is pushed the Tilapia mossambica intraperitoneal after, extract syringe needle out.
Through implementing common mark 8147 tail Tilapia mossambicas, the survival rate in a week behind the mark reaches more than 99%, excludes the influence of the lethality in breeding process, and the effective discrimination of PIT mark after 1 year reaches more than 97%.
Claims (1)
1. the method for a marking tilapia individual is characterized in that: adopt following processing step:
Earlier mark and syringe thereof were soaked in thimerosal 1～15 minute, described thimerosal is: 70～75% alcohol; With anaesthetic Tilapia mossambica is anaesthetized, described anaesthetic caryophyllus oil solution percentage concentration is: 0.01%～0.02%; The mark that to sterilize places in the syringe needle again, and syringe needle pierces through stomach wall from abdomen rib one side near Tilapia mossambica anus front portion with 45 degree, pushes intraperitoneal along stomach wall, and the length that described syringe needle enters the Tilapia mossambica abdominal cavity is 0.5～1.0cm; Treat that syringe needle enters the abdominal cavity, touch injector syringe, mark is pushed the Tilapia mossambica intraperitoneal after, extract syringe needle out;
The described PIT mark that is labeled as, the PIT mark is a kind of microchip of being discerned by radio frequency, and outsourcing glass is corynebacterium, and size is 11.50mm X 2.12mm, and the identification frequency is 128kHz.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100186679A CN101238800B (en) | 2008-03-05 | 2008-03-05 | Method for marking tilapia individual |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100186679A CN101238800B (en) | 2008-03-05 | 2008-03-05 | Method for marking tilapia individual |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101238800A CN101238800A (en) | 2008-08-13 |
| CN101238800B true CN101238800B (en) | 2011-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100186679A Expired - Fee Related CN101238800B (en) | 2008-03-05 | 2008-03-05 | Method for marking tilapia individual |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102077794A (en) * | 2010-12-10 | 2011-06-01 | 上海海洋大学 | Individual marking method for use in selective breeding of grass carps |
| CN102657117A (en) * | 2012-04-28 | 2012-09-12 | 江苏省淡水水产研究所 | Family building and good family breeding method for channel catfish |
| CN102657118B (en) * | 2012-05-04 | 2013-12-04 | 中国水产科学研究院淡水渔业研究中心 | Method for researching compensatory growth in process of fish-family seed selection |
| CN103222433A (en) * | 2013-01-28 | 2013-07-31 | 中国水产科学研究院淡水渔业研究中心 | Individual marking method of takifagu obscurus |
| CN104335934A (en) * | 2013-12-30 | 2015-02-11 | 中国科学院海洋研究所 | Method for marking abalone individual |
| CN105213484A (en) * | 2015-11-09 | 2016-01-06 | 阮少康 | The anesthetis of Fish wholefood composition |
| CN106962254A (en) * | 2017-03-17 | 2017-07-21 | 广东海洋大学 | A kind of sparid class batch denotation approach preferred based on mark effect quantitatively |
| CN107318739A (en) * | 2017-07-04 | 2017-11-07 | 浙江海洋大学 | A kind of abyssal fishes metal magnetic line mark |
| CN107251857A (en) * | 2017-07-10 | 2017-10-17 | 广东海洋大学 | A kind of ultrasonic transmitter denotation approach of fish |
-
2008
- 2008-03-05 CN CN2008100186679A patent/CN101238800B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 洪波等.标志放流技术在渔业中的应用现状及发展前景.《水产科技情报》.2006,第33卷(第2期),73-76. * |
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|---|---|
| CN101238800A (en) | 2008-08-13 |
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