CN101190238B - Method for using shrimp shell mud fermentation to produce biological calcium product - Google Patents
Method for using shrimp shell mud fermentation to produce biological calcium product Download PDFInfo
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- CN101190238B CN101190238B CN2006101283037A CN200610128303A CN101190238B CN 101190238 B CN101190238 B CN 101190238B CN 2006101283037 A CN2006101283037 A CN 2006101283037A CN 200610128303 A CN200610128303 A CN 200610128303A CN 101190238 B CN101190238 B CN 101190238B
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- lactobacillus bulgaricus
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Abstract
The invention discloses a method used for producing bio-calcium products through shrimp shell mud fermentation. The method comprises the following steps: washing, grinding, chylosis, sterilizing, inoculating strains, fermenting, separating, drying, detecting and preparing. The bio-calcium produced by the invention has the advantages of improving the solubility of the calcium and the absorptivity of the calcium after the calcium enters the human bodies, enhancing the bioavailability of the calcium in the shrimp shell, using plenty of waste shrimp shells and solving the problem of environmental pollution caused by the waste shrimp shells at the same time.
Description
Technical field
The present invention relates to a kind of method of producing calcium product, particularly a kind of method of utilizing Crusta Penaeus seu Panulirus mud microbial fermentation to produce biological calcium product.
Background technology
Calcium is the necessary element of a kind of life, also is the abundantest magnanimity metallic element of content in the human body.Calcium is the important component that constitutes human body, accounts for the 1.5-2% of body weight for humans.The major function of calcium is to constitute skeleton and tooth, and calcium accounts for 39.9% of its mineral in the skeleton, and calcium is to the normal development of skeleton and keep bone health crucial effects is arranged.
The a lot of countries in the whole world have entered aged tendency of population, and osteoporosis is one of first three big disease of infringement senior health and fitness, and osteoporosis causes just because of calcium deficiency.With China is example, one 999 year China entered aging society, at present person in middle and old age's population has broken through 1.43 hundred million, the Shanghai latest survey show China more than 60 years old the senile osteoporosis sickness rate be 56%, concurrent fracture person is up to 12%, China's population is owing to reasons such as diet structures, the intake wretched insufficiency of calcium shows according to national nutriture investigation for the third time, in China's population dietary intake, for the most obvious, China's intake of calcium per capita only is 50% of recommended amounts with the shortage of calcium.China's sufferers of osteoporosis face is about 10,000 ten thousand people, accounts for 7% of total number of persons.Existing calcium preparation mostly is organic salt calcium and inorganic salt calcium, and these calcium preparation have calcium hydrogen phosphate, calcium carbonate, 2-Butenedioic acid (E)-, calcium salt, Radix Asparagi calcium chlorate, calcium citrate, calcium acetate, calcium lactate, calcium gluconate, threonic acid calcium etc.
Calcium mainly enters human body cell by three kinds of modes: 1) calcium ion depends mainly on calcium ion concentration; 2) nonionic diffusion calcium (molecular calcium), cell membrane is selected to absorb to its biological nature; 3) calcium is in conjunction with plasma protein, mainly is that albumin and globulin combine with calcium and absorb.
The commercially available product of replenishing the calcium is many because of the factor that influences absorption of human body, poor effect.Be rich in calcium and several mineral materials in the Crusta Penaeus seu Panulirus, it is particularly necessary to go out to be easy to the microorganism calcium that organism-absorbing tires high as development of raw materials.
With the Xinyang is example, and its water resource is abundant, and fish and shrimp product quantity is big, and is wide in variety, is one of main place of production of Procambarus clarkii and astacus, about 10000 tons of annual production.With local maximum Aquatic product export enterprise is example, ton surplus year processing freshwater crayfish and the astacus 7000, ton surplus the discarded Crusta Penaeus seu Panulirus 5000, these Crusta Penaeus seu Panulirus garbages have become the environmental protection public hazards, utilize the Crusta Penaeus seu Panulirus processing fent, adopt bionic microbial enrichment method, develop microorganism calcium efficiently, meet the requirement of twice laid, environment-protecting clean production.
The literature search relevant with the application is as follows:
The file of patent No. CN1084028 discloses a kind of manufacture method of high nutrition shrimp calcium tablet, is the Macrobrachium nipponensis oven dry is pulverized; Crusta Penaeus seu Panulirus is pulverized with water extraction, filtration, vacuum concentration, oven dry; Fresh undrawn Crusta Penaeus seu Panulirus oven dry is pulverized; These three kinds of powders are mixed the tabletting that sieves with sodium chloride, micropowder silica gel, magnesium stearate and starch to form.
It is the method that raw material extracts the calcium hydroxide activated calcium with eggshell, Crusta Penaeus seu Panulirus or shell that the file of patent No. CN1082003 discloses a kind of, with the material after the cleaning sorting, carbonization 3-12 hour, carry out activation burning at 800-1700 ℃ of high temperature again, add distilled water, hydrolytic swelling in 200-300 ℃ of environment, oven dry, micronization can get 200 order Pi calcium hydroxide powder, and calcium content is the 48-57% activated calcium.
The file of patent No. CN78488 discloses a kind of calcium tablet with calcium extracted from shrimp shell and head, mainly form by following composition and percentage by weight, biological calcium carbonate 35%-45%, defatted milk powder 20%-30%, ferrous lactate 1%-3%, vitamin D 0.001%-0.005%, sweeting agent 31%-40%, additive 2%-6%, sweeting agent are (by the percentage ratio of gross weight) glucose 10%-13%, mannitol 10%-12%, cyclamate 0.5%-1%, citric acid 0.5%-2%, additive are (by the percentage ratio of gross weight) magnesium stearate 1%-2%, microcrystalline Cellulose 1%-4%.
More than the calcium product made of disclosed several method because wherein calcium exists with a kind of natural formation, so the utilization that is difficult for being absorbed by the body.
The file of patent No. CN1715255 discloses a kind of method of utilizing Crusta Penaeus seu Panulirus to produce chitin, astaxanthin, protein, calcium powder and bio-feritlizer, processing step is: Crusta Penaeus seu Panulirus is pulverized back adding compound enzyme carry out enzymolysis, after adding organic solvent extraction, abundant reaction then, be separated into shrimp liquid and shrimp solid, shrimp liquid carries out separatory, be divided into hydrolyzed solution and organic liquor, the organic liquor vacuum concentration of astaxanthin-containing is extracted thick astaxanthin, after hydrolyzed solution is removed organic solvent, carry out vacuum concentration, spray drying gets protein powder then; In the hydrochloric acid with shrimp solid immersion 3%, obtain the chitin finished product after the flushing, acid pickle is by COsub2/sub gas, obtain white precipitate, filtering drying gets activated calcium powder, and crude product astaxanthin liquid enters the ultrafilter ultrafiltration, obtain astaxanthin, waste water after the production adds phytic acid and active oligosaccharide, obtains organic nutrient solution for cultivating, makes bio-feritlizer.Though this technology has to prepared using fully, advantage such as good product quality owing to adopted organic solvent in the technology, can cause to a certain degree pollution to environment, control requires height to technical process simultaneously.
So above-mentioned various be that the calcium product of raw material exists shortcomings such as the molten rate of calcium is low, assimilation effect difference more with shrimp and Crusta Penaeus seu Panulirus, and the not enough environmental protection of production process etc.
Summary of the invention
In order to overcome the above problems, the invention provides the rich calcium of a kind of employing microbial method, by the fermentation of protein promotion probiotic bacteria, soluble amino acid and peptide content are improved, and then promote the dissolving of calcium, raising enters the absorbance behind the human body.
To achieve these goals, the invention provides a kind of method of shrimp shell mud fermentation to produce biological calcium product, may further comprise the steps:
Step 1, pulverizing: Crusta Penaeus seu Panulirus is cleaned up, with half machine of cutting with the Crusta Penaeus seu Panulirus micronizing;
Step 2, chylosis: colloid mill chylification homogenizing;
Step 3, sterilization: steam sterilization;
Step 4, inoculation strain: inoculating lactic acid bacterium and yeast;
Step 5, fermentation: control temperature at 35 ℃-38 ℃, pH6.2-7.0, fermentation time 30-45 hour;
Step 6, separation: adopt the fermentation liquid after membrance separation ferments, keep supernatant;
Step 7, drying.
Lactobacillus is a Lactobacillus bulgaricus in the described step 4.
Yeast is a cereuisiae fermentum in the described step 4.
Described Lactobacillus bulgaricus is preferably Lactobacillus bulgaricus.lb (AS.1.1480) and Lactobacillus Fermentum Beijerinck (AS.1.122).
Described cereuisiae fermentum is preferably Saccharomyces cerevisiae Hansen (AS.2.14) and Saccharomyces carlsbergensis Hansen (AS.2.441).
Inoculum concentration is 3%-5% in the described step 4.
Fermentation temperature is preferably 37 ℃ in the described step 5.
Fermentation time is preferred 36 hours in the described step 5.
Drying means is vacuum drying or spray drying in the described step 7.
Further comprising the steps of when forming preparation: step 8, detection: as to detect content of beary metal and microbiological indicator; Step 9, to make type be liquid, tablet or capsular preparation.
Compare former product, the present invention adopts the calcium absorptivity after the rich calcium of microbial method has improved the molten rate of the calcium in the goods and entered human body, has promoted the bioavailability of calcium in the Crusta Penaeus seu Panulirus.Promote the fermentation of probiotic bacteria soluble amino acid and peptide content to be improved by protein, promote the dissolving of calcium, improved the absorbance that enters behind the human body, making the calcium particle average diameter by the probiotics fermention effect is below the 2.5 μ m, helps the Digestive system effect.
Simultaneously, the high-effective microorganism calcium product that the present invention obtains also has following advantage, and the one, calcium content height, calcium content are greater than 35%, and the calcium dissolution rate is greater than 80%; The 2nd, absorbance and bioavailability height utilize greater than 90%, and absorbance is greater than 45%; The 3rd, safe without toxic side effect, content of beary metal does not exceed standard, and every index meets the GB14880 nutrition enhancer and uses standard, is a kind of comparatively ideal calcium-nutrition intensifying agent.
Also have, the invention solves depleted a large amount of Crusta Penaeus seu Panulirus of year, the traditional processing method of these Crusta Penaeus seu Panulirus is the raw materials for production as chitin, and the production of chitin needs the processing of strong acid and strong base, the serious pollution of ecological environment of emission will be prohibited, and buries facture, time-consuming taking a lot of work, occupy cultivated land and may produce stench, the Crusta Penaeus seu Panulirus garbage has become the environmental protection public hazards.Utilize the leftover bits and pieces Crusta Penaeus seu Panulirus of Procambarus clarkii processing, adopt the biological concentration method, develop microorganism calcium efficiently, meet twice laid, the requirement of cleaner production.
Description of drawings
Fig. 1 is the flow chart of the method for the invention
The specific embodiment
Embodiment 1:
Procambarus clarkii is clean with flushing with clean water, flush away impurity, excrement dirt, to reduce contamination by micro, with half machine of cutting with the Crusta Penaeus seu Panulirus micronizing, colloid mill chylification homogenizing, the base material of rotten refinement is inserted in the TBS biological fermentation tank, and rotten thin purpose is to be beneficial to microbial growth and fermentation and raising calcium to hold rate; Behind steam sterilization, kill the assorted bacterium in the Crusta Penaeus seu Panulirus culture medium, in order to the enrichment of the growth and breeding and the calcium of probiotic bacteria; The base material cooling below 40 ℃, is added the necessary nutrient substance of probiotic bacteria growth, and regulating PH with sodium hydrogen phosphate, sodium dihydrogen phosphate or phosphoric acid is 6.5-7.0.By TSB fermentation tank inoculation mouth, inoculate Lactobacillus bulgaricus (as: Lactobacillus bulgaricus.lb (AS.1.1480) and Lactobacillus Fermentum Beijerinck (AS.1.122)) and cereuisiae fermentum (as: Saccharomyces cerevisiae Hansen (AS.2.14) and Saccharomycescarlsbergensis Hansen (AS.2.441)) after the activated amplification, inoculum concentration is the 3-5% of (W/W) of cultivation material, control 35-38 ℃ of its fermentation temperature, stir speed (S.S.) is 150-180r/min, keep suitable ventilation, making and cultivating material is aerobic environment.Fermenting added appropriate amount of defoamer with control foam after 10 hours, after fermentation in 30-45 hour is finished,
Earlier use the liquid object filtering separation device, remove the fermentation filtering residue, the microfiltration assembly operation pressure 0.04Mpa-0.1Mpa membrane technology separating and filtering that reuse 2.5um is following, the fermentation filtrate is made the microorganism calcium powder with vacuum drying or spray drying.Detect the microorganism calcium content greater than 35%, the calcium dissolution rate is greater than 80%, and absorbance is greater than 45%, and detects heavy metal physicochemical property and microbiological indicators such as arsenic, lead, hydrargyrum, makes it to meet the sanitary standard of GB14880 nutrition enhancer.Follow-uply carry out local flavor debugging and make dosage forms such as microorganism Ca oral liquid, tablet or capsule.
As the step among the embodiment 1, its fermentation parameter can be selected following arbitrary combination:
| Inoculum concentration | Fermentation temperature ℃ | Fermentation time (hour) | ?pH | |
| Combination 1 | ?3％ | 35 | ?45 | ?6.2 |
| Combination 2 | ?5％ | 38 | ?37 | ?7.0 |
| Combination 3 | ?4％ | 37 | ?36 | ?6.9 |
It should be noted last that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to the foregoing description, will be understood by those skilled in the art that: still can make amendment or be equal to replacement the present invention, and do not break away from any modification or partial replacement of the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (8)
1. the method for a shrimp shell mud fermentation to produce biological calcium product is characterized in that may further comprise the steps:
Step 1, pulverizing: with the Crusta Penaeus seu Panulirus micronizing;
Step 2, chylosis: colloid mill chylification homogenizing;
Step 3, sterilization: steam sterilization;
Step 4, inoculation strain: inoculation Lactobacillus bulgaricus and cereuisiae fermentum;
Step 5, fermentation: control temperature at 35 ℃-38 ℃, pH6.2-7.0, fermentation time 30-45 hour;
Step 6, separation: adopt the fermentation liquid after membrance separation ferments, keep supernatant;
Step 7, drying.
2. method according to claim 1, wherein said Lactobacillus bulgaricus are Lactobacillus bulgaricus.1b and Lactobacillus Fermentum Beijerinck.
3. method according to claim 1, wherein said cereuisiae fermentum are Saccharomycescerevisiae Hansen and Saccharomyces carlsbergensis Hansen.
4. method according to claim 1, inoculum concentration is 3%-5% in the wherein said step 4.
5. method according to claim 1, fermentation temperature is 37 ℃ in the wherein said step 5.
6. method according to claim 1, fermentation time is 36 hours in the wherein said step 5.
7. method according to claim 1, drying means is vacuum drying or spray drying in the wherein said step 7.
8. method according to claim 1, further comprising the steps of when wherein forming preparation:
Step 8, detection: detect content of beary metal and microbiological indicator;
Step 9, to make type be liquid, tablet or capsular preparation.
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| CN2006101283037A CN101190238B (en) | 2006-11-30 | 2006-11-30 | Method for using shrimp shell mud fermentation to produce biological calcium product |
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| CN2006101283037A CN101190238B (en) | 2006-11-30 | 2006-11-30 | Method for using shrimp shell mud fermentation to produce biological calcium product |
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| CN101190238B true CN101190238B (en) | 2011-04-13 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101731478B (en) * | 2010-01-20 | 2012-05-09 | 中国水产科学研究院淡水渔业研究中心 | Production method of fermented shrimp shell meal added Cambarus proclarkii special feed |
| CN105249464B (en) * | 2015-11-18 | 2018-02-23 | 江南大学 | The one chelated calcium preparation method of seed shrimp peptide |
| CN108634188A (en) * | 2018-06-28 | 2018-10-12 | 山东仁迪生物工程集团有限公司 | A kind of calcium nutritional supplement, high calcium flour and wheaten food |
| CN109527440A (en) * | 2018-11-15 | 2019-03-29 | 韶关市华工高新技术产业研究院 | It is a kind of to be starched using the method and manufactured fish-bone of extruding and puffing technology production high active calcium fish-bone slurry |
| CN112957375B (en) * | 2021-04-07 | 2023-04-07 | 江西师范大学 | High-affinity shrimp shell calcium powder, preparation method thereof and application thereof in preparing medicines for treating calcium deficiency symptoms and/or diseases |
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| CN1032732C (en) * | 1992-09-14 | 1996-09-11 | 贾文成 | Method for producing high nutritious prawn calcium tablet |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1032732C (en) * | 1992-09-14 | 1996-09-11 | 贾文成 | Method for producing high nutritious prawn calcium tablet |
Non-Patent Citations (6)
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| 刘文宗 等.酵母菌、乳酸菌复合发酵奶的开发研究.食品科学22 3.2001,22(3),62-64. |
| 刘文宗等.酵母菌、乳酸菌复合发酵奶的开发研究.食品科学22 3.2001,22(3),62-64. * |
| 赵玉红 等.乳酸菌和酵母菌共生发酵生产面包的研究.食品工业科技24 3.2003,24(3),61-62. |
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