CN101167497A - Milk containing thymus polypeptide extraction and preparation method thereof - Google Patents
Milk containing thymus polypeptide extraction and preparation method thereof Download PDFInfo
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- CN101167497A CN101167497A CNA2007101786045A CN200710178604A CN101167497A CN 101167497 A CN101167497 A CN 101167497A CN A2007101786045 A CNA2007101786045 A CN A2007101786045A CN 200710178604 A CN200710178604 A CN 200710178604A CN 101167497 A CN101167497 A CN 101167497A
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Abstract
The invention discloses a peptide-containing milk and process for preparation. The milk of the invention is processed through charging thymus polypeptide extract extracted from fetal bovine thymus tissue into common milk, and then processing through homogeneous process. During preparing the thymus polypeptide extract, steps of homogenate, freezing and thawing, interception, filtering and the like are employed, thereby processing thymus polypeptide extract with molecular weight < 30000 Daltons. A lot of experiments prove that the peptide-containing milk of the invention has the functions of enhancing immunity and stimulating stem cells to proliferate.
Description
Technical field
The present invention relates to a kind of peptide breast and preparation technology thereof, particularly a kind of milk and preparation technology thereof who contains thymus polypeptide extraction.
Background technology
On Chinese traditional ancient medicine dietotherapy and herbal cuisine are just arranged, continue into the present, existing thousands of years.Flourishing industry has brought negative effect when promoting socioeconomic progress, the deterioration of living environment, the aggravation of air and pollution of waterhead, nervous allegro modernized life style, invisible has promoted malignant tumour, hyperlipidemia, hypertension, artery sclerosis, diabetes, the existence of the generation of obesity etc. and a large amount of sub-health populations.Stimulating people to pay close attention to self health and existence more, needing some special functional food to regulate body function, increasing resistance disease.Health food is a kind of novel food, and at home and abroad rising is nearly 20 years new things.Yet in short 20 years, the development of health food is very swift and violent, and both nutrition can also disease resistance, also becomes the focus of people's growing interest.Study effective health product, can reduce the huge medical expenses of society; And the energy prevent disease, mediator's body immunity function is the effective way of benefiting the nation and the people.
Milk is one of the most frequently used food of the most nutritious and people, is again the best natural emulsion simultaneously, can be used as desirable emulsion of many nutriments, medicine and other bioactivator or fortification carrier.Though the mankind have been developed many milk class functional foods, the relevant research that adds tire ox thymus polypeptide extraction in milk yet there are no the research report.
Summary of the invention
The object of the invention is to disclose a kind of method for preparing thymus polypeptide extraction; Another purpose of the present invention is to disclose a kind of milk that contains the said extracted thing and preparation method thereof.
The present invention seeks to be achieved through the following technical solutions:
The raw material of peptide breast of the present invention consists of:
Milk 100 parts by volume, thymus polypeptide extraction 0.1～2 parts by volume.
The raw material composition of peptide breast of the present invention is preferably:
Milk 100 parts by volume, thymus polypeptide extraction 0.5 parts by volume.
The raw material composition of peptide breast of the present invention is preferably:
Milk 100 parts by volume, thymus polypeptide extraction 0.25 parts by volume.
The raw material composition of peptide breast of the present invention is preferably:
Milk 100 parts by volume, thymus polypeptide extraction 1.5 parts by volume.
Wherein the preparation method of thymus polypeptide extraction is as follows:
Collect tire ox thymic tissue; Fresh or freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3～5 times of aseptic ultra-pure waters, make homogenate; The aseptic ultra-pure water that adds 3～8 times of volumes, mixing is put-10 ℃～-30 ℃ multigelations 2～3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 10～30 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, get the daltonian thymus polypeptide extraction in molecular weight＜30000; Aseptic filtration; Promptly.
Wherein the preparation method of thymus polypeptide extraction is preferably:
Collect tire ox thymic tissue; Fresh or freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3～5 times of aseptic ultra-pure waters, make homogenate; The aseptic ultra-pure water that adds 5 times of volumes, mixing is put-20 ℃ of multigelations 2～3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 20 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, get the daltonian part in molecular weight＜30000, promptly get thymus polypeptide extraction; Carry out aseptic filtration; Promptly.
To be lower than 90 daltonian content of peptides be 49.4%～58.3% to molecular weight in the thymus polypeptide extraction, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian content of peptides are 7.1～12.9%.
According to a conventional method above-mentioned thymus polypeptide extraction is joined in the fresh milk, make the peptide breast.
Peptide breast of the present invention is a kind of health, safety and the new type functional food with certain medical function, drinks peptide breast of the present invention, can improve the intestinal microecology environment, and the toxin of pathogen and spoilage organisms and generation thereof promotes body health in the removing enteron aisle.Peptide breast of the present invention is safe and harmless to human body, can not exert an influence to some beneficial microbes in the enteron aisle, the many side effects that do not have antibiotic to have can not make normal flora unbalance, cause the bigger endurance strain of toxigenicity etc., have good economic benefits and social benefit.The present invention is a raw material with tire ox thymic tissue, extracts thymus polypeptide extraction and joins in the milk, is prepared into the immune peptide breast, and adopts effective function assessment assay method it to be strengthened functions such as immunity, stimulation stem cells hyperplasia and estimated.Good application prospects is arranged on market, will contribute for the mankind's public health work.
Following experimental example is used to further specify but is not limited to the present invention:
Experimental example 1 thymus polypeptide extraction of the present invention is to stem cell and the experiment of lymphocytic proliferation function, result of the test such as table 1, the lymphocytic proliferation rate of 1: 100,1: 200,1: 400,1: 800,1: 1,600 five diluted concentration groups of thymus polypeptide extraction all is significantly higher than blank group, can effectively promote lymphopoiesis.
Table 1 thymus polypeptide extraction is to the influence (external) of lymphopoiesis vigor
| Extension rate | The OD value | The rate of increase |
| Dilution in 1: 100 dilution in 1: 200 dilution in 1: 400 dilution in 1: 800 dilution in 1: 1600 is blank | 1.388±O.136 1，2 1.197±O.108 1，2 1.216±0.07 1，2 1.237±O.166 1，2 1.316±0.091 1，2 O.847±O.036 | 63.9％ 41.3％ 43.6％ 46.O％ 55.4％ - |
Annotate: 1.P＜0.05, to compare with the blank group, difference all has statistical significance; Person's appreciation rate height that contains the thymus polypeptide extraction; 2.P＞0.05, difference not statistically significant between the different diluted concentrations.
The process and the result of the experiment of experimental example 2 peptides breast Function Identification
90 of SPF level kunming mices, in 4～6 ages in week, male and female half and half are divided into 3 groups at random, 30 every group.The A group gives the peptide breast and irritated stomach in 0.5ml/ days; The B group gives plain chocolate 0.5ml/ days filling stomaches, and the C group gives physiological saline 0.5ml/ days filling stomaches.Behind the continuous irrigation stomach 15 days, adjust initial cell number about 0.7 * 10
6/ ml observes the mouse lymphocyte conversion test with cell counting; Behind the continuous irrigation stomach 30 days, observe the mouse lymphocyte conversion test with mtt assay; Behind the continuous irrigation stomach 90 days, observe mouse lymphocyte conversion test, result such as table 2 with mtt assay.
Table 2 peptide breast is to the influence of mouse lymphocyte proliferation activity
| Group | The cell incubation time | 15 days (cell count * 10 6) | 30 days (mtt assay OD value) | 90 days (mtt assay OD value) |
| A group B group C group | 72h 72h 72h | 3.726±0.722 1，21.246±0.239 30.837±0.319 | 0.179±O.021，2 0.158±0.007 3 O.142±O.022 | 0.274±0.053 1，2 O.187±0.0039 3 0.158±O.053 |
Annotate: 1.A group and B group be P＜O.05, compare with common plain chocolate group relatively, and difference has statistical significance, and the present invention contains peptide breast appreciation rate height; 2.A group compares P＜0.05 with the C group, compares with the physiological saline group, difference has statistical significance, and the present invention contains peptide breast rate of increase height; 3.B group compares P＞0.05 with the C group, compares with the physiological saline group, the difference not statistically significant can not think that ordinary milk and physiological saline appreciation rate have difference.
Following embodiment all can realize the described effect of above-mentioned experimental example.
The specific embodiment
Embodiment 1:
Collect tire ox thymic tissue; Fresh tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 4 times of aseptic ultra-pure waters of volume, make homogenate; Add the aseptic ultra-pure water of 5 times of volumes, mixing is put-20 ℃ of multigelations 2 times; The homogenate of freeze thawing under 4～8 ℃ of temperature, is left the heart 20 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, the daltonian thymus polypeptide extraction in molecular weight＜30000; To be lower than 90 daltonian content of peptides be 49.4%～58.3% to molecular weight in the thymus polypeptide extraction, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian content of peptides are 7.1～12.9%; Aseptic filtration, promptly; The 1ml thymus polypeptide extraction is joined in the 200ml sweet milk, the packing, the peptide breast.
Embodiment 2:
Collect tire ox thymic tissue; Freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 5 times of aseptic ultra-pure waters of volume, make homogenate; The aseptic ultra-pure water that adds 4 volumes, mixing is put-15 ℃ of multigelations 3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 20 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, the daltonian thymus polypeptide extraction in molecular weight＜30000; To be lower than 90 daltonian content of peptides be 49.4%～58.3% to molecular weight in the thymus polypeptide extraction, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian content of peptides are 7.1～12.9%; Aseptic filtration; Promptly; The 0.5ml thymus polypeptide extraction is joined in the 200ml sweet milk, the packing, the peptide breast.
Embodiment 3:
Collect tire ox thymic tissue; Fresh tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3 times of aseptic ultra-pure waters of volume, make homogenate; The aseptic ultra-pure water that adds 7 volumes, mixing is put-25 ℃ of multigelations 2 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 20 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, the daltonian thymus polypeptide extraction in molecular weight＜30000; To be lower than 90 daltonian content of peptides be 49.4%～58.3% to molecular weight in the thymus polypeptide extraction, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian content of peptides are 7.1～12.9%; Aseptic filtration; Promptly; The 3ml thymus polypeptide extraction is joined in the 200ml sweet milk, the packing, the peptide breast.
Claims (7)
1. a milk that contains thymus polypeptide extraction is characterized in that it is by containing thymus polypeptide extraction 0.1～2 parts by volume in the 100 parts by volume milk; Wherein thymus polypeptide extraction is prepared by following method: collect tire ox thymic tissue; Fresh or freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3～5 times of aseptic ultra-pure waters, make homogenate; The aseptic ultra-pure water that adds 3～8 times of volumes, mixing is put-10 ℃～-30 ℃ multigelations 2～3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 10～30 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, get the daltonian thymus polypeptide extraction in molecular weight＜30000; Aseptic filtration; Promptly.
2. milk as claimed in claim 1 is characterized in that containing in the 100 parts by volume milk thymus polypeptide extraction 0.25,0.5 or 1.5 parts by volume.
3. the preparation method of milk as claimed in claim 1 or 2 is characterized in that according to a conventional method thymus polypeptide extraction being joined in the fresh milk, makes the peptide breast.
4. the preparation method of milk as claimed in claim 3 is characterized in that may further comprise the steps: collect tire ox thymic tissue; Fresh or freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3～5 times of aseptic ultra-pure waters, make homogenate; The aseptic ultra-pure water that adds 3～8 times of volumes, mixing is put-10 ℃～-30 ℃ multigelations 2～3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 10～30 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, get the daltonian thymus polypeptide extraction in molecular weight＜30000; Aseptic filtration; Promptly; Thymus polypeptide extraction is joined in the fresh milk, make the peptide breast.
5. the preparation method of milk as claimed in claim 4 is characterized in that wherein the preparation of thymus polypeptide extraction may further comprise the steps: fresh or freezing tire ox thymic tissue is at room temperature cleaned with ultra-pure water; Add 3～5 times of aseptic ultra-pure waters, make homogenate; The aseptic ultra-pure water that adds 5 times of volumes, mixing is put-20 ℃ of multigelations 2～3 times; The homogenate of freeze thawing 4～8 ℃ of temperature, is left the heart 20 minutes with per minute 8000, remove precipitation; Hold back supernatant with cross-flow ultrafiltration equipment, get the daltonian part in molecular weight＜30000, promptly get thymus polypeptide extraction; Carry out aseptic filtration; Promptly.
6. as the preparation method of claim 4 or 5 described milk, it is characterized in that wherein in the thymus polypeptide extraction that it is 49.4%～58.3% that molecular weight is lower than 90 daltonian content of peptides, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian polypeptide and little molecular regulation protein content are 7.1～12.9%.
7. milk as claimed in claim 1 or 2, it is characterized in that wherein in the thymus polypeptide extraction that it is 49.4%～58.3% that molecular weight is lower than 90 daltonian content of peptides, 90～130 daltonian content of peptides are 10.6%～12.9%, 130～270 daltonian content of peptides are that 21.7%～27.1%, 270～30000 daltonian content of peptides are 7.1～12.9%.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101786045A CN101167497A (en) | 2007-12-03 | 2007-12-03 | Milk containing thymus polypeptide extraction and preparation method thereof |
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| CNA2007101786045A CN101167497A (en) | 2007-12-03 | 2007-12-03 | Milk containing thymus polypeptide extraction and preparation method thereof |
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| CN101167497A true CN101167497A (en) | 2008-04-30 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450356A (en) * | 2013-09-23 | 2013-12-18 | 河南牧翔动物药业有限公司 | Preparing method of high-purity thymosin |
| CN105541996A (en) * | 2015-12-31 | 2016-05-04 | 天津泰创生物科技有限公司 | Method for extracting thymosin, medium molecular thymus protein and DNA from thymus tissue |
| CN108125140A (en) * | 2017-12-11 | 2018-06-08 | 长春三真实业有限公司 | A kind of calf internal organs extract frozen dried food that can improve the elderly's body's immunity |
| CN108125139A (en) * | 2017-12-11 | 2018-06-08 | 长春三真实业有限公司 | A kind of thymic extract frozen dried food that can improve the elderly's body's immunity |
| CN108633998A (en) * | 2018-05-09 | 2018-10-12 | 安徽勤蜂堂蜂业有限公司 | A kind of preparation method of honey milk |
| CN109568345A (en) * | 2019-01-04 | 2019-04-05 | 湖南海济药业有限公司 | A kind of strengthen immunity, oral solution of anti-aging and preparation method thereof |
-
2007
- 2007-12-03 CN CNA2007101786045A patent/CN101167497A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103450356A (en) * | 2013-09-23 | 2013-12-18 | 河南牧翔动物药业有限公司 | Preparing method of high-purity thymosin |
| CN105541996A (en) * | 2015-12-31 | 2016-05-04 | 天津泰创生物科技有限公司 | Method for extracting thymosin, medium molecular thymus protein and DNA from thymus tissue |
| CN108125140A (en) * | 2017-12-11 | 2018-06-08 | 长春三真实业有限公司 | A kind of calf internal organs extract frozen dried food that can improve the elderly's body's immunity |
| CN108125139A (en) * | 2017-12-11 | 2018-06-08 | 长春三真实业有限公司 | A kind of thymic extract frozen dried food that can improve the elderly's body's immunity |
| CN108633998A (en) * | 2018-05-09 | 2018-10-12 | 安徽勤蜂堂蜂业有限公司 | A kind of preparation method of honey milk |
| CN109568345A (en) * | 2019-01-04 | 2019-04-05 | 湖南海济药业有限公司 | A kind of strengthen immunity, oral solution of anti-aging and preparation method thereof |
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| C41 | Transfer of patent application or patent right or utility model | ||
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| TA01 | Transfer of patent application right |
Effective date of registration: 20080314 Address after: Shandong province Caoxian Yinxiang wulidun Albert company Applicant after: Wang Yinxiang Address before: Shandong province Caoxian Yinxiang wulidun Albert company Applicant before: Yu Zhongbo |
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| C41 | Transfer of patent application or patent right or utility model | ||
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Effective date of registration: 20080718 Address after: Wuli Dun Yin Xiang Weiye company, Caoxian, Shandong, zip code: 274400 Applicant after: Shandong silvery earth and dairy industry Co., Ltd. Co-applicant after: Wang Yinxiang Address before: Shandong Province Caoxian Wulidun silver incense Albert CHAN company Applicant before: Wang Yinxiang |
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Open date: 20080430 |