CN101147751A

CN101147751A - Method for extracting biologically active component from momordica grosvenori plant

Info

Publication number: CN101147751A
Application number: CNA2006100512185A
Authority: CN
Inventors: 周英; 黄赤夫
Original assignee: 周英
Current assignee: Department of Hubei special biomedical Technology Co., Ltd.
Priority date: 2006-09-19
Filing date: 2006-09-19
Publication date: 2008-03-26

Abstract

The present invention discloses a method for extracting bio-active components from Grosvenor's momordica plant. Said method includes the following steps: mechanically pulverizing dried Grosvenor's momordica leaf, stem, root and fruit to obtain the Gosvenor's momordica crude powder or crushing the fresh Gosvenor's momordica plant to obtain wet broken blocks, soaking the above-mentioned crude powder or broken blocks in clear water or ethyl alcohol liquor or methyl alcohol liquor and heating to make extraction, filtering the obtained extract mixture, then making the filtrate be passed through macroporous adsorbing resin to make chromatographic separation, making elation and collecting eluents respectively so as to obtain the required active components.

Description

Extract the method for biological active component from Momordica grosvenori plant

Technical field

The present invention relates to the bioactive ingredients of natural materials, in particular to extracting method with the Momordica grosvenori plant biological active component.

Background technology

Momordica grosvenori plant is that the perennial perennial root of Cucurbitaceae is climbed up by holding on to liana Fructus Momordicae (Momordicagrosvenori), Fructus Momordicae is mainly cultivated in Guangxi of China the north, in south China, all there is distribution in Guizhou, Jiangxi and other many areas, plantation, the applicating history in existing more than 300 year, it really has the title of " Fructus Momordicae ".Chinese medicine and pharmacy is thought, Fructus Momordicae is sweet, sour, cool in nature, clearing away heat and cooling blood is arranged, effects such as the cough-relieving of promoting the production of body fluid, laxation toxin expelling, skin care benefit face, moistening the lung and resolving phlegm, can be used for life prolonging, preserve youthful looks Yue Se and treatment phlegm-heat cough, laryngopharynx swelling and pain, constipation, the irritated all diseases of quenching one's thirst.Since the fifties in last century, the fruit of Fructus Momordicae has been carried out the research of a lot of active component, by these research, separate and identified very strong sweet ingredient in the fruit of Fructus Momordicae, i.e. Momordia grosvenori aglycone (I is to VI).Momordia grosvenori aglycone is a triterpenoid compound, and its glucose ring links to each other with the mogrol base.One piece of patent to relevant Momordia grosvenori aglycone in the bioactivity research report of Lo Han Guo fruit is pointed out Momordia grosvenori aglycone, and particularly mogroside has the activity (U.S. Pat Patent 6 319 523) of anti-oral microorganism.Yet, but do not have the report of related science document to confirm for the antimicrobial acivity of this Momordia grosvenori aglycone.

The fruit of Fructus Momordicae is normally plucked in autumn end, and meanwhile, the rattan of Momordica grosvenori plant is just cut down, and leaf and rattan are dropped.Its root still is embedded in the soil, and it passes the winter soil protection.At present people are to the almost few of research of non-fruit part of Momordica grosvenori plant, and its leaf and rattan also never are used, for the biological activity of the non-fruit part of its plant and utilize people to know little about it, and also any activated extract not from wherein obtaining.

Summary of the invention

The object of the present invention is to provide a kind of method of extracting biological active component from Momordica grosvenori plant.

The inventor has discovered the effective ingredient with antimicrobial acivity in Momordica grosvenori plant, and this effective ingredient is not known Momordia grosvenori aglycones that are present in the Lo Han Guo fruit of people; The antimicrobial acivity composition of being found is present in Folium Momordicae, rattan, root, stem and the fruit, and the strongest antimicrobial acivity position is the nonpolar part of Folium Momordicae, rattan, root, stem and fruit extract.These antimicrobial acivities mainly show the activity of anti-oral microorganism, such as cause the streptococcus of dental caries, porphyromonas gingivalis and candida mycoderma etc.The antimicrobial acivity that the inventor predicts Momordica grosvenori plant can be used to prepare antimicrobial extract and medicine (such as cough syrup, the medicine of treatment laryngopharynx swelling and pain, toothpaste and collutory etc.), and this will have very big practical value and economic worth.

For this discovery is brought benefit to the mankind, the inventor explores the technology of extracting the antimicrobial acivity effective ingredient from Momordica grosvenori plant.Through studying repeatedly, testing, invented extraction from Folium Momordicae, rattan, root, stem and fruit, separated, the method for purification antimicrobial bioactive substance.The method of being invented is that the step of passing through column chromatography of laboratory from Momordica grosvenori plant and industrially scalable is extracted and had the antimicrobial bioactive substance.Its concrete grammar comprises:

1. be that leaf, rattan, root, stem and fruit carry out mechanical activation comminution and obtain coarse powder with the various piece of prior drying in the sun Momordica grosvenori plant, or the fragment that the leaf of fresh Momordica grosvenori plant, rattan, root, stem and fruit crushing are obtained wetting, soak described coarse powder and fragment with the ethanol water of clear water or 50% or 50% methanol aqueous solution, and be heated at least 50 ℃, carry out the above extraction of 30min, obtain extract liquid;

2. will extract mixture filters, filtrate is at room temperature separated with chromatography method by macroporous adsorbent resin, and macroporous adsorptive resins carries out gradient elution or methanol aqueous solution gradient elution with the ethanol of clear water, reuse 10%% to 100% behind the material that goes not adsorb through washing; Collect 30% and 60% to 100% eluent respectively, promptly obtain required active part; Maybe will extract mixture and filter, filtrate is separated by silica gel column chromatography, carries out the gradient elution collection by chloroform/ethanol/water gradient elution or chloroform/methanol/water and obtains active part;

3. the filtrate drying is obtained the crude extract of active Momordica grosvenori plant, this crude extract contains the active component of antimicrobial acivity.

Above-mentionedly be used for carrying out isolating macroporous adsorbent resin and silica gel comprises the commercially available various apertures and the resin and the silica gel of model, as macroporous adsorbent resin of the DM series of Amberlite XAD series and Lukang Medical Co., Ltd., Shandong etc.

The present invention finds first and provides from Folium Momordicae, rattan, root, extraction in stem and the fruit, separate, method that purification has antimicrobial active substance, because leaf, stem, rattan, the root past of Fructus Momordicae are depleted, therefore utilize the plant of Fructus Momordicae to develop the antimicrobial extract of preparation, and make it to be applied to medicine and functional food, article of everyday use and food additive etc. will have very big practical value and economic worth.

The specific embodiment

In 500ml water, boil 30min after embodiment 1100g Folium Momordicae grinds and extract, extract and repeat 3 times.Extracting liquid filtering is obtained the about 1200ml of filtrate.The filtrate drying obtains the active Folium Momordicae crude extract of 7g, and this crude extract contains certain antimicrobial active active component.

In 500ml water, boil 30min after embodiment 2100g Folium Momordicae grinds and extract, extract and repeat 3 times; Extracting liquid filtering is obtained the about 1200ml of filtrate.Filtrate had been carried out isorrheic nonpolar Amberlite XAD post by 100ml; Wash to remove the not material of absorption with 1000ml.Each 100ml of ethanol of reuse 10% to 100% carries out gradient elution; Contain our needed active substance in 30% and 60% to 100% the eluent, collect 30% and 60% to 100% eluent respectively, the eluent drying with 60% to 100% obtains the antimicrobial activity extract of having of 2.5g.

In 500ml water, boil 30min after embodiment 3100g Lo Han Guo fruit grinds and extract, extract and repeat 3 times; Extracting liquid filtering is obtained the about 1200ml of filtrate; Filtrate had been carried out isorrheic nonpolar Amberlite XAD post by 100ml; Wash to remove the not material of absorption with 1000ml; Each 100ml of ethanol of reuse 10% to 100% carries out gradient elution, contain our needed active substance in 30% and 60% to 100% the eluent, collect 30% and 60% to 100% eluent respectively, the eluent drying with 60% to 100% obtains the antimicrobial activity extract of having of 2.1g.

In 250ml water, boil 30min after embodiment 450g arhat stem end grinds and extract, extract and repeat 3 times; Extracting liquid filtering is obtained the about 600ml of filtrate; Filtrate had been carried out isorrheic nonpolar Amberlite XAD post by 50ml.Wash to remove the not material of absorption with 500ml; Each 50ml of ethanol of reuse 10% to 100% carries out gradient elution; Contain our needed active substance in 30% and 60% to 100% the eluent, collect 30% and 60% to 100% eluent, the eluent drying with 60% to 100% obtains the antimicrobial activity extract of having of 1.25g.

In 250ml water, boil 30min after embodiment 550g Momordica grosvenori root grinds and extract, extract and repeat 3 times.Extracting liquid filtering is obtained the about 600ml of filtrate; Filtrate had been carried out isorrheic nonpolar Amberlite XAD ion exchange column by 50ml; Wash to remove the not material of absorption with 500ml; Each 50ml of ethanol of reuse 10% to 100% carries out gradient elution, contain our needed active substance in 30% and 60% to 100% the eluent, collect 30% and 60% to 100% eluent, the eluent drying with 60% to 100% obtains the antimicrobial activity extract of having of 1.25g.

In 250ml water, boil 30min after embodiment 650g Folium Momordicae grinds and extract, extract and repeat 3 times; Extracting liquid filtering is obtained the about 600ml of filtrate; Concentrated filtrate, sample on dry method or the wet method.Carry out 50ml silica gel column chromatography post, extract and carry out gradient elution with chloroform/ethanol/water, wherein the ratio of water increases gradually from 1, concrete eluant ratio is: 40: 5: 1 (50ml), 35: 5: 1 (50ml), 30: 8: 1 (50ml), 25: 10: 5 (50ml), 20: 10: 5 (50ml), 10: 10: 20 (50ml) collects the 300ml eluent; Obtain the antimicrobial activity extract of having of 1.25g with obtaining the eluent drying.

Claims

1. one kind is extracted the method for biological active component from Momordica grosvenori plant, and its feature comprises:

1. be that leaf, rattan, root, stem and fruit carry out mechanical activation comminution and obtain coarse powder with the various piece of prior drying in the sun Momordica grosvenori plant, or the fragment that the leaf of fresh Momordica grosvenori plant, rattan, root, stem and fruit crushing are obtained wetting, soak described coarse powder and fragment with the ethanol water of clear water or 50% or 50% methanol aqueous solution, and be heated at least 50 ℃, carry out the extraction more than 30 minutes, obtain crude extract;

2. will extract mixture and filter, filtrate is at room temperature separated with chromatography method by macroporous adsorbent resin, and macroporous adsorptive resins is behind the material that goes not adsorb through washing; The ethanol of reuse 10% to 100%/or methanol carry out eluting, collect 30% and 70% to 100% eluent respectively, promptly obtain required active part; Maybe will extract mixture and filter, filtrate is separated by silica gel column chromatography, collects by chloroform/ethanol/water gradient elution and obtains active part;

2. extract the method for biological active component according to claim 1 is described from Momordica grosvenori plant, it is characterized in that describedly being used for carrying out isolating macroporous adsorbent resin and silica gel comprises the commercially available various apertures and the resin or the silica gel of model.