CN101138315A

CN101138315A - Barley recovery system breeding method

Info

Publication number: CN101138315A
Application number: CNA200710133986XA
Authority: CN
Inventors: 张明生; 张彦平
Original assignee: Jiangsu Institute of Coastal Area Agricultural Sciences
Current assignee: Jiangsu Institute of Coastal Area Agricultural Sciences
Priority date: 2007-10-26
Filing date: 2007-10-26
Publication date: 2008-03-12

Abstract

The present invention relates to a restoring line breeding method of the barley combination hybridized by robust and high-quality ones; the method belongs to the technical field of agricultural crop breeding. The method comprises that the two-rowed barley restoring line is taken as the maternal plant and multi-rowed barley as the paternal plant; individual plants with scattered fringe and good flowering habit in the F2 generation to inbreed for 3 to 5 generations and get the restoring line 238; the restoring line 238 is taken as the maternal plant to hybridize with the multi-rowed barley 35 as the paternal plant; the offspring is backcrossed with the 35 paternal plant for 3 to 5 generations and get the flowering restoring line 03-358 with excellent agriculture features. The fringes of the restoring line 03-358 are scattered which is beneficial to long-term loose powder; the blossom is 2 to 3 days which is beneficial to adjusting the parent plants blossoms to meet; the anther is hung outside in blossom which is beneficial to hybridization. The parent 270A is taken as the maternal plant and the restoring line 03-358 is taken as the paternal plant; the combination is hybrid one of strong advantages; the hybrid F1 generation is two-rowed one; the largest fringe has 33 grains and the dry grain weight is about 45 grams; the stem is hard which is lodging resistant and the advantage of the hybrid is strong.

Description

A kind of selection of barley recovery system

One, technical field

The present invention relates to a kind of recovery system selective breeding method of strong excellent hembar combination, belong to the crop breeding technical field, it is exclusively used in the production of high-yield hybrid barley seed in the hybrid seeds of barley use of advantage.

Two, background technology

At present, also do not solve the technological difficulties in the hybrid seeds of barley use of advantage both at home and abroad well, as the selection of male sterile line, maintenance line and recovery system, the screening of strong excellent hybrid combination, the high yield technique of hybrid seeding etc.We find the barley male sterile material in nineteen eighty-three, bred the stable barley male sterile line of sterile proterties " close 270A " in 1993, and realization three series mating, filter out " salt 94-258 " strong excellent hembar combination in 1997～1999 years and participate in Jiangsu Province's barley regional test, the conventional check variety volume increase about 10% of its production ratio, filter out " salt 97-11 " strong excellent hembar combination to calendar year 2001 in 1999 and participate in Jiangsu Province's barley regional test, filter out " salt 99-256 " strong excellent hembar combination in 2001 to 2003 and participate in Jiangsu Province's barley regional test, the conventional check variety volume increase about 10% of production ratio, our research work is taken its place in the front ranks of the world, but still does not reach the level of application.How to utilize barley male sterile line " close 270A " to filter out strong excellent hembar combination, key is to select good recovery system, and wants its flowering habit good, hybrid yield heterosis height, crossbreed strong stress resistance.We select the strong excellent hembar combination of 05-378 through research for many years, and it recovers system is 03-358, and through preliminary test, this combination yield heterosis is obvious, first generation of hybrid good stress resistance.

Three, summary of the invention

1, technical problem

It is not strong to The present invention be directed in the hybrid seeds of barley use of advantage hybrid vigour, problems such as cross breeding seed output is not high propose a kind of selection of being of recovering, and the recovery cording that is brought out has first generation of hybrid output height, recovery is that flowering habit is good, helps hybrid seed yield and improves.

2, technical scheme

The breeding method of a kind of barley recovery system provided by the present invention comprises:

1) recovering with two-rowed barley is 777 be female parent, and two-rowed barley parent 270 be a paternal hybrid, its F2 in the thin type fringe of selection, flowering habit be out the individual plant that grain husk is bloomed, allow its 3-5 for selfing, the offspring selects flowering habit to be out the material that grain husk is bloomed, and obtains two-rowed barley and recovers material 238: dredge the type fringe, the rib type is two ribs, and awns is arranged, and skin is arranged, plant height 70cm, spike length 9cm, number of grain per ear 28, thousand kernel weight 45g, open grain husk and bloom, the plant type compactness, economical character is with parent 270;

2) carry out group gas-mixing hybridization with the recovery material 238 of above-mentioned acquisition and multi-rowed barley 35 male parents of reflecting, offspring and multi-rowed barley 35 male parents of reflecting are backcrossed the 3-5 of selfing afterwards generation, select many ribs fringe individual plant, the plant performance of selected individual plant is stable, dredges the type fringe, four ribs, awns is arranged, skin is arranged, plant height 70cm, spike length 9cm, number of grain per ear 60, thousand kernel weight 29g, flowering habit is bloomed for opening grain husk, economical character is with mirror 35, and it is 03-358 that the self progeny is the recovery that the present invention obtains.

In the breeding method that a kind of good recovery provided by the present invention is, being female parent with close 270A, is that 03-358 is a male parent with the recovery that obtains, and is the strong combination of hybrid vigour.

3, beneficial effect

The method of the present invention by two or four ribs hybridization selects that barley male sterile line parent 270A is had the recovery of restitution is 03-358, has the following advantages and good effect:

1) dredges four ribs.In the fringe type of barley, the rib type has the branch of two ribs and many ribs (four ribs and six ribs), and the fringe type has the branch of dense cluster and thin fringe.For the ease of the production of hybrid seeds, recovery system should preferentially select to dredge the type fringe and be four rib fringes, and dredging the recovery of type fringe is that flowering habit is good, and four rib tassel blossom phases are long, and pollen amount is big, helps improving cross breeding seed output like this.It is 03-358 that the present invention breeds recovery, and the fringe type is to dredge four ribs, and flower pesticide is plug-in when blooming, plant height 70cm, anti-elimination of barley's yellow mosaic.Recovery is that 03-358 fringe type is dredged, and little on the same tassel the flowers are in blossom florescence difference helps the long period loose powder, in 2-3 days flowering stage, helps regulating this flower synchronization of father and mother; Flowering habit is good, and flower pesticide is plug-in when blooming, and helps hybrid seeding; About plant height 70cm, about 60 of every fringes are about thousand kernel weight 29 grams.

2) advantage is strong.Barley two rib fringes belong to two different subspecies with many ribs fringe (four ribs, six ribs) in classification, barley male sterile line parent 270A is two rib fringes, recovery is that 03-358 is many ribs fringe, is that the 03-358 combo is an inter-subspecies hybrid with male sterile line parent 270A with recovery, and its first generation of hybrid shows advantages such as big panicle many grains per panicle, lodging resistance are strong.With close 270A is female parent, to have good economical character and the present invention that bloom breeds that to recover be that 03-358 recovers be that male parent is carried out combo, detects the yield heterosis of its F1, and hybrid vigour is strong.First generation of hybrid 05-378 is two rib fringes, plant height 80.5cm, and 33 of maximum fringes, about thousand kernel weight 45 grams, stalk is hard, and is resistant to lodging, and hybrid vigour is strong.

Four, description of drawings

Fig. 1 is breeding pedigree and the seed selection process of 03-358 for recovering

Five, embodiment

(1) 1992 year field combo, female parent be recover to be 777 (public material, document sees reference: Zhang Mingsheng etc., seed selection and the value of barley male sterile line parent 270A, Scientia Agricultura Sinica, 1999 1 phases, 96-98), male parent is parent's 270 (public materials, document sees reference: Zhang Mingsheng etc., seed selection and the value of barley male sterile line parent 270A, Scientia Agricultura Sinica, 1999 1 phases, 96-98).21 crossbreed of kind in 1993.

Select thin type fringe, flowering habit to be out that grain husk is bloomed, the good offspring with parent 270 of economical character: to select 3 strains, summer nineteen ninety-five to select 11 strains in summer in 1994.Select 12 strains in summer in 1996, selected 9 strains in summer in 1997; Flowering habit is bloomed for opening grain husk, and obtaining the two-rowed barley recovery is 238: the rib type is two ribs, and awns has perverse, and skin is arranged, plant height 70cm, spike length 9cm, 28 of every fringes, thousand kernel weight 45g, flowering habit is bloomed for opening grain husk, the plant type compactness, blade length is moderate, and economical character is good, with parent 270.

Recovery is 777: two ribs, the skin wheat, and the awns spinosity, 70 centimetres of plant heights, 10.2 centimetres of spike lengths, about 25 of every fringes, thousand kernel weight 44 grams, blade is more roomy, opens grain husk and blooms, and with male sterile line parent 270A hybridization, its first-filial generation can be educated, and is the recovery system of male sterile line parent 270A.

270: two ribs of parent, the skin wheat, the awns spinosity, 60.5 centimetres of plant heights, 7.8 centimetres of spike lengths, about 20 of every fringes, thousand kernel weight 45 grams are opened grain husk and are bloomed, and with male sterile line parent 270A hybridization, its first-filial generation flower pesticide abortion is the maintenance line of male sterile line parent 270A.

(2) 1998 year spring the field combo, recovering with two-rowed barley is 238 to be female parent, with multi-rowed barley mirror 35 (public materials, document sees reference: dagger-axe and waiting quietly, the excavation and the evaluation in the anti-source of barley head blight, wheat crops journal 2007,27 (1): 153-157) be male parent, carry out artificial hybridization, autumn sowing field numbering W11 in 1998.Spring W11 was maternal in 1999, was male parent with multi-rowed barley mirror 35, backcrossed autumn sowing in 1999.

Multi-rowed barley mirror 35: rib type six ribs, the awns spinosity, naked property, 73 centimetres of plant heights, 6 centimetres of spike lengths, number of grain per ear 60, thousand kernel weight 35 grams close grain husk and bloom.Hybridize with male sterile line parent 270A, the first generation of hybrid is sterile, is that the hybridization first generation of hybrid can be educated with recovering, and can import in the recovery system spending more many graininesses shape.

Selected many ribs fringe, the economical character individual plant of mirror 35 together in (3) 2000 to 2002 in the self progeny: summer harvest b79 obtained to be numbered the b43 individual plant in 2002, the plant performance of b43 individual plant is stable, dredges the type fringe, four ribs, awns is arranged, skin is arranged, plant height 70cm, spike length 9cm, number of grain per ear 60, thousand kernel weight 29g, flowering habit is bloomed for opening grain husk, and economical character is with mirror 35.

(4) 2003 year spring, the field combo was a female parent with male sterile line parent 270A, was that male parent is carried out assembly hybrid combination with b43, and the summer in 2004 mixed and receives fringe b43.Field numbering b43 is named and is 03-358 during autumn sowing in 2003, its crossbreed field numbering W228.Spring W228 showed big fringe in 2004, and rib type two ribs have awns, the skin wheat, and flowering habit is out that grain husk blooms, plant height 80.5cm, spike length 10cm, average 28 of every fringe, thousand kernel weight 45g, advantage is stronger.

(male sterile line parent 270A sees reference document: Zhang Mingsheng etc., the feature and the utilization of barley male sterile line " close 270A ", Jiangxi agricultural journal, 2000 1 phases, 28-32): rib type two ribs, awns is arranged, skin wheat, plant height 57cm, spike length 8cm, number of grain per ear 20, thousand kernel weight 46g, flowering habit are out that grain husk blooms.Resistant to lodging, Chinese People's Anti-Japanese Military and Political College's wheat yellow mosaic.

(5) continuing with male sterile line parent 270A simultaneously is female parent, is that male parent is carried out assembly hybrid combination with 03-358, autumn sowing field numbering W05-378 in 2005, and it shows big fringe, 33 of maximum fringes, average 28, plant height 80.5cm, output height.Small size is amounted to and is reached 990 gram/m ²,, i.e. 660 kilograms of per mu yields are than other combination amount of increase in production big (table 1).

Table 12006 year summer harvest crossbreed is surveyed and produces

The field numbering	Male parent numbering last year	Male parent rib type	Plant height/cm	Output gram/0.15m ²	Amount to output/(kilogram/mu)
The field numbering	Male parent numbering last year	Male parent rib type	Plant height/cm	Output gram/0.15m ²	Amount to output/(kilogram/mu)	05-257	150	Two	70.5	82.8	368
05-306	171	Many	81	101.3	450	05-257	150	Two	70.5	82.8	368
05-306	171	Many	81	101.3	450	05-330	210	Two	80	124.6	554
05-351	290	Two	83	110.9	493	05-330	210	Two	80	124.6	554
05-351	290	Two	83	110.9	493	05-354	291	Two	89.5	73.7	328
05-364	307	Two	77.5	92.8	412	05-354	291	Two	89.5	73.7	328
05-364	307	Two	77.5	92.8	412	05-378	358	Many	80.5	148.5	660

Claims

1. the breeding method of a barley recovery system comprises:

1) recovering with two-rowed barley is 777 be female parent, and two-rowed barley parent 270 be a paternal hybrid, its F2 in the thin type fringe of selection, flowering habit be out the individual plant that grain husk is bloomed, allow its 3-5 for selfing, the offspring selects flowering habit to be out the material that grain husk is bloomed, and obtains two-rowed barley and recovers material 238: dredge the type fringe, the rib type is two ribs, and awns is arranged, and skin is arranged, plant height 70cm, spike length 9cm, number of grain per ear 28, thousand kernel weight 45g, flowering habit is good, the plant type compactness, and economical character is with parent 270;

2) carry out group gas-mixing hybridization with the recovery material 238 of above-mentioned acquisition and multi-rowed barley 35 male parents of reflecting, offspring and multi-rowed barley 35 male parents of reflecting are backcrossed back selfing 3-5 generation many ribs of selection fringe individual plant among the self progeny, the plant performance of selected individual plant is stable, dredges the type fringe, four ribs, awns is arranged, skin is arranged, plant height 70cm, spike length 9cm, number of grain per ear 60, thousand kernel weight 29g, flowering habit is bloomed for opening grain husk, economical character is with mirror 35, and being the recovery that the present invention obtains is 03-358.

2. the described barley recovery system 03-358 of claim 1 is the hybrid combination of female parent as male parent, with close 270A.