Summary of the invention
Based on the above-mentioned problems in the prior art, the present invention is according to the selection of chlorophyllous absorption spectrum and light emitting diode, a kind of method of led light source coordinate plant growth and technical scheme of device thereof utilized is provided, this method can improve photosynthesis of plants, night, indoor or cloudyly all can carry out light filling irradiation to plant.
The described method of utilizing the led light source coordinate plant growth is characterized in that selecting to utilize the mixed light of different LED light source that plant is shone, and to promote plant growing, following method is pressed in the mixed light combination of the selection of led light source and different LED light source:
1) according to the plant chlorophyll absorption spectrum, spectral wavelength that select to determine plant growing is had facilitation is 660 ± 30nm, 460 ± 20nm, three wave bands of 400 ± 20nm, thereby determines to utilize red LED, blue led and purple LED to be radiation source;
2) according to the absorption energy ratio of plant chlorophyll to three kinds of light, determine to form a mixed light pixel cell by 2 red LED, 1 blue led, 1 purple LED, blue led and purple LED are arranged on cornerwise two summits of pixel cell, 2 red LED are arranged on another cornerwise two summits of pixel cell, and determine the operating current of redness, blueness and purple LED;
3) with step 2) pixel cell combination arrange, constitute the mixed light combination of different LED light source, by the mixed light of different LED plant is shone.
The corollary apparatus of described method according to claim 1, it is characterized in that the pixel cell of being arranged by combination connects by circuit and overall package constitutes, comprise constant voltage source device, reference voltage device, constant-current source device, transformer T in the described constant voltage source device
1Output and rectifier bridge D
1Connect rectifier bridge D
1Dc output end is through capacitor C
1Filtering is connected with the input of adjustable linear voltage stabilization chip U1, and the output of adjustable linear voltage stabilization chip U1 is connected with 2 pin of voltage stabilizing chip U3, and 6 pin of voltage stabilizing chip U3 are connected with the input of constant-current source device.Transformer T in the described reference voltage device
2Output and rectifier bridge D
2Connect rectifier bridge D
2Dc output end is through capacitor C
3Filtering is connected with the input of adjustable linear voltage stabilization chip U2,1 pin of constant current chip U4 in the output of adjustable linear voltage stabilization chip U2 and the constant-current source device is connected, described constant-current source device is by one group of constant current chip U4 and connect and constitute, and the input of constant current chip U4 connects resistance R
12With adjustable resistance R
13, be connected on the output pin of corresponding constant current chip U4 according to the operating current of redness, blueness and purple LED.
Described corollary apparatus is characterized in that described adjustable linear voltage stabilization chip U1, adjustable linear voltage stabilization chip U2 adopt the LM317 chip, and voltage stabilizing chip U3 adopts the MAX6175 chip, and constant current chip U4 adopts the MAX1916 chip.
Described corollary apparatus is characterized in that described adjustable linear voltage stabilization chip U1, adjustable linear voltage stabilization chip U2 output connect filter capacitor C respectively
2, C
4
Described corollary apparatus is characterized in that described voltage stabilizing chip U3 input connects filter capacitor C
5
The above-mentioned method of utilizing the led light source coordinate plant growth, novel in design, design uniqueness, utilize LED as radiation source, according to plant chlorophyll absorption spectrum and luminous flux, simulating plant optimum growh photoenvironment, improve the illumination condition of crops, promote photosynthesis of plant, make plant not be subjected to time, season and natural climate condition restriction to be in the optimum growh state all the time.Described corollary apparatus provides red, blue, purple three color contamination light for plant continuously by the illuminator of self, match with the photosynthesis of plant absorption spectrum, illumination brightness is suitable, satisfy the needs of photosynthesis of plant, personnel bring great convenience for cultivation, and low cost of manufacture, energy consumption are little, stable working state, reliability height, long service life, help market and popularize, apply.
Embodiment
The invention will be further described below in conjunction with Figure of description:
Figure 1 shows that the plant chlorophyll abosrption spectrogram that botanists record, transverse axis is a wavelength, the longitudinal axis is an absorbance, as seen from the figure, chlorophyll mainly concentrates on the ruddiness about the 660nm of visible light to the absorption of sunshine, near the blue light and the purple light about 400nm about 460nm, and the ratio that estimates the peak energy of plant chlorophyll absorption spectrum redness (about 660nm), blue (about 460nm), purple (about 400nm) is 60: 39: 18.
Utilize the method for led light source coordinate plant growth, it is manual simulation plant optimum growh photoenvironment, selection utilizes the mixed light of different LED light source that plant is shone, improve the illumination condition of crops, to promote plant growing, following method is pressed in the mixed light combination of the selection of led light source and different LED light source:
1) according to the plant chlorophyll absorption spectrum, spectral wavelength that select to determine plant growing is had facilitation is 660 ± 30nm, 460 ± 20nm, three wave bands of 400 ± 20nm, thereby determines to utilize red LED, blue led and purple LED to be radiation source;
2) according to the absorption energy ratio of plant chlorophyll to three kinds of light, determine by 2 red LED, 1 blue led, 1 purple LED forms a mixed light pixel cell, as shown in Figure 2, blue led and purple LED are arranged on cornerwise two summits of pixel cell, 2 red LED are arranged on another cornerwise two summits of pixel cell, and definite red LED operating current is 20mA, operating current blue and purple LED is 12mA, make redness (660 ± 30nm), blue (460 ± 20nm) with purple (ratio of the peak energy of 400 ± 20nm) LED is 60: 39: 18, meet the absorption energy ratio of plant chlorophyll to three kinds of light, the mixed light pixel cell also can be by 2 red LED, 2 blue leds constitute;
3) with step 2) pixel cell combination arrange, constitute the mixed light combination of different LED light source, by the mixed light of different LED plant is shone.
The above-mentioned corollary apparatus that utilizes the method for led light source coordinate plant growth, the pixel cell of being arranged by combination constitutes by circuit connection and overall package, comprises constant voltage source device 1, reference voltage device 2, constant-current source device 3.As shown in Figure 3, the 220V alternating current of input passes through transformer T in the constant voltage source device 1
1After obtain alternating voltage to human body safety, as the 36V alternating current, transformer T
1Output and rectifier bridge D
1Connect rectifier bridge D
1Dc output end is through capacitor C
1Filtering is connected with the input of adjustable linear voltage stabilization chip U1, and adjustable linear voltage stabilization chip U1 adopts the LM317 chip, by changing R
1And R
2Ratio relation obtain regulated output voltage 36V at output, its output is connected with 2 pin of voltage stabilizing chip U3, U3 provides 36V operating voltage for the voltage stabilizing chip, at adjustable linear voltage stabilization chip U1 output filter capacitor C in parallel
2, voltage stabilizing chip U3 input filter capacitor C in parallel
5Can improve the stability of voltage stabilizing chip U3 input voltage more, voltage stabilizing chip U3 adopts the MAX6175 chip, and its 6 pin is connected with the input of constant-current source device 3, for follow-up constant-current source device 3 provides reference voltage 5V.The 220V alternating current of input is by transformer T in the reference voltage device 2
2After obtain the 9V alternating current, transformer T
2Output and rectifier bridge D
2Connect rectifier bridge D
2Dc output end is through capacitor C
3Filtering is connected with the input of adjustable linear voltage stabilization chip U2, and adjustable linear voltage stabilization chip U2 adopts the LM317 chip, by changing R
3And R
4Ratio relation obtain the 5V regulated output voltage at output, 1 pin of the constant current chip U4 in its output and the constant-current source device 3 is connected, U4 provides 5V operating voltage for the constant current chip, at adjustable linear voltage stabilization chip U2 output filter capacitor C in parallel
4Can improve the stability of its output voltage more.Constant-current source device 3 is by one group of constant current chip U4 and connect and constitute, and constant current chip U4 adopts the MAX1916 chip, and its input connects resistance R
12With adjustable resistance R
13, require to regulate adjustable resistance R according to the control of output current
13Carry out dividing potential drop to adjust the voltage V of constant current chip U4
Set, make constant current chip U4 require to regulate the electric current that is fit to redness, blueness and purple LED work according to the control of output current.Redness, blueness and purple LED are connected on the output pin of corresponding constant current chip U4 according to the operating current of setting, and the red LED operating current is 20mA, and operating current blue and purple LED is 12mA.The spectral curve of apparatus of the present invention as shown in Figure 4.
The above-mentioned method of utilizing the led light source coordinate plant growth and device thereof can be widely used in the crops booth and comprise that fruit, melon, leaf vegetables, flowers, fruit tree, group train, grow seedlings etc., play the purpose of quickening plant growth, promote the crop precocity, the average maturing stage can shift to an earlier date 10~15 days, output improves 20~30%, the sugar content of fruit on average improves more than 10%, also can be used as the additional light source that interior decoration gardens, the healthy growth that helps flowers and trees also has the effect of interior decoration lamp simultaneously concurrently.Compare with the logical sodium plant growth lamp of the high light that adopts on the market, though luminescent spectrum all matches with the photosynthesis of plant absorption spectrum, illumination brightness is suitable, satisfy the needs of photosynthesis of plant, but device of the present invention obviously has advantage such as low cost of manufacture, energy consumption are little, stable working state, reliability height, long service life.
Below for adopting the logical sodium vapor lamp of device of the present invention and high light respectively paddy rice, arabidopsis to be compared the record of exposure experiment:
Test one: paddy rice exposure experiment
1, experiment material
Rice varieties: japonica rice 941 (Japonica), by the training of basin pot culture, basin alms bowl diameter 27cm needs A, B two basins.The about 15kg of every basin dress soil is livid purple mud for examination soil, measures content of organic matter 47.3g/kg, full nitrogen 2.3g/kg, alkali-hydrolyzable nitrogen 162.4g/kg, rapid available phosphorus 16.61g/kg, available potassium 135.5g/kg, pH6.45 according to the soil basic fertility.
2, experimental procedure
In the equal transplantation of seedlings engagement of the paddy rice seedling alms bowl with 28 days length of time rice seedlings grow, placing temperature is 26-28 ℃, and relative air humidity is in about 70% the culturing room, adopts apparatus of the present invention, the logical sodium vapor lamp of high light to carry out the dark alternately illumination cultivation of 14h illumination/8h respectively.Carry out the water and fertilizer management and the extermination of disease and insect pest according to the paddy growth characteristics.
3, the mensuration of rice plant physiologically active
3.1 the mensuration of chlorophyll content in leaf blades
Adopt measuring chlorophyll content to adopt spectrophotometric method.Get fresh rice leaf, 3 in each sample is parallel, use the absorbent cotton wiped clean, shred mixing, take by weighing about 0.2g, grind to form pasty state with 95% ethanol, washing and filtering bleaches fully until blade then, constant volume is in the scale test tube of 30ml, leaching liquor immediately 665,649nm place colorimetric, record absorbance (OD) value.Computing formula: CA=13.95OD665-6.88OD649, CB=24.96OD649-7.32OD665, in the formula, CA, CB are respectively the concentration of chlorophyll a and b, and unit is mg L-1, chlorophyll content (mg g-1)=(C * V)/(W * 1000), in the formula, C i.e. (CA+CB) is chlorophyll total concentration (mg L-1), and W is fresh weight (g), and test data sees Table 1.
3.2 the mensuration of plant fresh weight and plant height
Rice shoot after the transplanting was surveyed rice plant fresh weight and plant height (height on the ground) every 10 days, test data sees Table 2 respectively, table 3.
Table 1: paddy rice exposure experiment measuring chlorophyll content record (mg g-1)
Test period (my god) |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
1.82±0.22 |
1.91±0.35 |
2.56±0.40 |
2.68±0.45 |
High light leads to sodium vapor lamp |
1.61±0.25 |
1.65±0.25 |
2.11±0.30 |
2.13±0.42 |
Table 2: paddy rice exposure experiment fresh weight is measured record (gram/strain)
Test period (my god) |
0 |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
50.0±5.26 |
68.0±6.20 |
112.0±9.55 |
205.0±16.0 |
252.0±17.0 |
High light leads to sodium vapor lamp |
50.0±5.20 |
61.0±6.00 |
95.0±8.50 |
181.0±11.5 |
221.0±15.0 |
Table 3: paddy rice exposure experiment fresh weight is measured record plant height (cm)
Test period (my god) |
0 |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
15.0±2.10 |
19.0±2.50 |
28.0±2.50 |
45.0±3.00 |
47.0±3.50 |
High light leads to sodium vapor lamp |
13.0±1.20 |
18.0±1.60 |
22.0±2.50 |
39.0±3.00 |
42.0±3.50 |
4, data analysing method and experimental result
Data statistics utilizes SAS software, carries out variance analysis and Fisher ' s significance test (LSD), relatively between different disposal in the significance of p<0.05.
Gross energy, press rating of set * light application time and calculate:
Apparatus of the present invention: W2=18*14*3600*40,
High light leads to sodium vapor lamp: W1=500*14*3600*40;
Effective energy, approximate think equal the plant growing amount, adopt the last fresh weight of rice shoot as effective energy:
Apparatus of the present invention: 252.00 ± 17.00,
High light leads to sodium vapor lamp: 221.00 ± 15.00;
Light efficiency, press effective energy/gross energy and calculate:
Apparatus of the present invention light efficiency: (252.00 ± 17.00/18*14*3600*40) * 100%,
High light leads to the sodium vapor lamp light efficiency: (221.00 ± 15.00/500*14*3600*40) * 100%;
Light efficiency ratio: apparatus of the present invention light efficiency: high light lead to the sodium vapor lamp light efficiency=
[(252.00±17.00/18*14*3600*40)*100%]∶
[(221.00±15.00/500*14*3600*40)*100%]≈31
That is: for monocotyledon, apparatus of the present invention light efficiency is approximately 33 times of the logical sodium vapor lamp light efficiency of high light.
Test two: arabidopsis exposure experiment
1, experiment material:
The environmental arabidopsis seed of WS capacity, vegetable garden soil, ash, wood sawdust capacity, two on pallet, 12 of culture plates.
2, experimental procedure:
The indoor simple and easy cultivation of arabidopsis is adopted in this experiment, preparation vegetable garden soil: ash: the culture matrix of wood sawdust=4: 2: 1, the culture matrix that mixes is packed in the Culture basin, Culture basin is positioned on the pallet, a pallet is put 6 Culture basins, is divided into A, two pallets of B.Adding water in the sowing forward direction pallet treats to sow behind the culture matrix surface wettability.After the seed fumigation, place low temperature treatment in 4 ℃ of refrigerators, sow behind the 2-4d with the sterile water immersion.For making seed broadcast evenly and improve speed of drilling, with seed with the aqueous suspension of 1gL-1 agar, with dropper the seed program request in stromal surface, the Culture basin of diameter 10cm is broadcast the 5-6 point, every program request 2-3 grain seed is broadcast the back with pricking the plastic film covering that ommatidium is arranged.Placing 21-23 ℃ and relative air humidity is about 70% culturing room, carries out artificial lighting and cultivates.The A pallet places under the logical sodium vapor lamp of high light and cultivates, and 14h illumination/8h is alternately dark.The B pallet places under apparatus of the present invention and cultivates, and 14h illumination/8h is alternately dark.Carry out the water and fertilizer management and the extermination of disease and insect pest according to the arabidopsis growth characteristic.
3, the mensuration of arabidopsis plant physiologically active
3.1 measuring chlorophyll content:
Measuring chlorophyll content employing spectrophotometric method [Wang Lei, Bai Youlu. different nitrogen are handled the correlative study of spring corn blade spectral reflectivities and full nitrogen of blade and chlorophyll content. Scientia Agricultura Sinica, 2005,38 (11)].Get fresh Arabidopsis leaf, 3 in each sample is parallel, use the absorbent cotton wiped clean, shred mixing, take by weighing about 0.2g, grind to form pasty state with 95% ethanol, washing and filtering bleaches fully until blade then, constant volume is in the scale test tube of 30ml, leaching liquor immediately 665,649nm place colorimetric, record absorbance (OD) value.Computing formula: CA=13.95OD665-6.88OD649, CB=24.96OD649-7.32OD665, in the formula, CA, CB are respectively the concentration of chlorophyll a and b, and unit is mg L-1, chlorophyll content (mg g-1)=(C * V)/(W * 1000), in the formula, C i.e. (CA+CB) is chlorophyll total concentration (mg L-1), and W is fresh weight (g), and test data sees Table 4.
Table 4: arabidopsis exposure experiment measuring chlorophyll content record (mg g-1)
Test period (my god) |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
1.22±0.11 |
2.4±0.35 |
3.0±0.40 |
3.56±0.10 |
High light leads to sodium vapor lamp |
1.02±0.20 |
1.95±0.25 |
2.99±0.30 |
3.30±0.40 |
3.2 the mensuration of plant fresh weight and plant height
After planting surveyed arabidopsis plant fresh weight and plant height (height on the ground) every 10 days, test data sees Table 5 respectively, table 6.
Table 5 arabidopsis exposure experiment fresh weight is measured record (gram/strain)
Test period (my god) |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
5.00±0.44 |
8.56±0.51 |
13.20±1.22 |
17.33±1.51 |
High light leads to sodium vapor lamp |
4.20±0.30 |
7.36±0.24 |
11.30±1.56 |
15.25±1.16 |
Table 6 arabidopsis exposure experiment plant height is measured record (cm)
Test period (my god) |
10 |
20 |
30 |
40 |
Apparatus of the present invention |
8.00±0.40 |
13.10±1.50 |
16.10±1.10 |
22.20±2.22 |
High light leads to sodium vapor lamp |
6.10±0.60 |
10.55±0.55 |
15.10±1.20 |
20.10±2.15 |
4 data analysing methods and result of the test
Data statistics utilizes SAS software, carries out variance analysis and Fisher ' s significance test (LSD), relatively between different disposal in the significance of p<0.05.
Gross energy, press rating of set * light application time and calculate:
Apparatus of the present invention: W2=18*14*3600*40,
High light leads to sodium vapor lamp: W1=500*14*3600*40;
Effective energy, approximate think equal the plant growing amount, adopt the last fresh weight of rice shoot as effective energy:
Apparatus of the present invention: 17.33 ± 0.51,
High light leads to sodium vapor lamp: 15.25 ± 0.16;
Light efficiency, press effective energy/gross energy and calculate:
Apparatus of the present invention light efficiency: (17.33 ± 0.51/18*14*3600*40) * 100%,
High light leads to the sodium vapor lamp light efficiency: (15.25 ± 0.16/500*14*3600*40) * 100%;
Light efficiency ratio: apparatus of the present invention light efficiency: high light lead to the sodium vapor lamp light efficiency=
[(17.33±0.51/18*14*3600*40)*100%]∶
[(15.25±0.16/500*14*3600*40)*100%]≈33
That is: for dicotyledon, apparatus of the present invention light efficiency is approximately 33 times of the logical sodium vapor lamp light efficiency of high light.
By above result of the test as can be seen: adopt the method for led light source coordinate plant growth and the device thereof of utilizing of the present invention can satisfy the normal photosynthetic light filling requirement of plant growing, and has a good energy-saving effect, and be cold light source, be not easy contingencies such as breaking out of fire in actual applications, therefore than traditional be that the plant growth lamp of light source has application prospect widely with the sodium vapor lamp.