CN101129433A - Method for extracting active component of edible burdock - Google Patents
Method for extracting active component of edible burdock Download PDFInfo
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- CN101129433A CN101129433A CNA2007101128712A CN200710112871A CN101129433A CN 101129433 A CN101129433 A CN 101129433A CN A2007101128712 A CNA2007101128712 A CN A2007101128712A CN 200710112871 A CN200710112871 A CN 200710112871A CN 101129433 A CN101129433 A CN 101129433A
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Abstract
The invention discloses an extracting method of active component of arctium lappa to prepare essential oil, soluble component and oligosaccharide, which is characterized by the following: using steam to distill the arctium lappa cut disposed by ultrasonic wave; obtaining the essential oil of arctium lappa; leaching the raw material through hot water step by step; extracting under negative pressure; pressing; decompressing and concentrating the extract; spraying to dry; obtaining the soluble component product; or using alcohol to sediment oligofructosan from the condensate; obtaining the product with content at 95%; simplifying the operation effectively as functional food, fodder additive and plant disease-resistant inducer.
Description
Affiliated technical field
The present invention relates to a kind of extracting method of natural plant functional components, specifically, be a kind of be raw material with wild and/or cultivation Radix Arctii, the industrialized producing technology of water-soluble component, quintessence oil and oligofructose in the preparation Radix Arctii.
Background technology
Fructus Arctii (Arctium lappa L.), it is Compositae biennial herb plant, be distributed widely in the Eurasia, China introduces cultigen the eighties from Japan, the present Cangshan, Pingdu, highly dense, Anqiu in Shandong, the Feng County in Jiangsu, Pei County, plant throughout the year on ground such as the Xiao Xian in Anhui, Dangshan, and area reaches 300,000 mu.The edible part of Fructus Arctii is its root, domestic cultivation Fructus Arctii main exit Japan.The international market is higher to outlet Fructus Arctii specification requirement, causes to wait outer root bulk deposition and discard.
The Fructus Arctii sweet in the mouth is flat nontoxic, have be good for the stomach, the effect of QI invigorating, YIN nourishing and YANG strengthening, heat-clearing and toxic substances removing; Can resist spiritual atrophy, prevent the sedimentation of fat, keep vessel wall elasticity, prevent hypertension, arteriosclerosis, and various types of rheumatic arthritis is had curative effect.It is main active as follows: (1) improves immunity, has antiviral, anticancer and press down function of tumor; (2) strengthen animal endurance, have significant strong effect; (3) animal pattern is had improve and the effect of maintenance ability; (4) hypoglycemic activity; (5) remove free radical, anti-aging effect; (6) antibacterial action, Fructus Arctii water decoction or water-leach liquor have certain antibacterial action, to staphylococcus aureus, homocentricity trichobacteria, microsporon audouini, epidermophyton inguinale, star-like slave blocks bacterium etc. all inhibitory action, can control not suppuration person of headache due to pathogenic wind-heat, laryngopharynx swelling and pain, Common Cold, furuncle toxic swelling, tinea pedis, eczema, scabies and subclinical mastitis; (7) prevention scarlet fever, tonsillitis, measles effect; (8) other effect is the paralysis effect to mobile nerve and skeletal muscle, has slight diuresis, discharge function etc.As seen, Fructus Arctii is a medicinal and edible plant preferably.
Burdock is abundant, is rich in outside saccharide, protein, aminoacid, multivitamin and the mineral, also contains abundant secondary metabolism active substance.The root of Fructus Arctii is rich in inulin class oligofructose, is a kind of oligosaccharide that is polymerized with β (2 → 1) glycosidic bond by the D-fructose of furan configuration, and its terminal connects a glucose molecule with α (1 → 2) glycosidic bond, and the degree of polymerization is 10-17, is no more than 18.Burdock fructooligosaccharidcan is a kind of functional food, can be used for developing the health food and the natural drug of anti-curing oncoma, coronary heart disease, diabetes, colon cancer, constipation etc.; Also might be as the plant disease-resistant elicitor, the self-defence immune system of activated plant is resisted disease and pest, is used for efficient, nontoxic, the non-harmful biological pesticide of development of new.
The secondary metabolism active substance of Radix Arctii, as alkaloid, aldehydes matter and phytosterol etc., may be relevant with the tonifying YANG and the antifatigue effect of Fructus Arctii.
Volatile ingredient in the Fructus Arctii quintessence oil extraction separation Fructus Arctii comprises kinds more than 20 such as aldehyde compound, terpenoid, alkane, alkynes vinyl compound, organic acid, ester compounds, has hair growth promoting, antiinflammatory, the anti-ageing effect of waiting for a long time.
The patent of extracting inulin from Radix Arctii is authorized, and the patent of invention that is called " edible burdock inulin and preparation method thereof " (patent No. is ZL03156256,6) as name has just disclosed a kind of preparation method of extracting edible burdock inulin from Fructus Arctii.But the processing step that this method adopts is comparatively loaded down with trivial details, and does not provide the means of extracting other active component in the Fructus Arctii, does not satisfy the needs that Fructus Arctii is carried out comprehensive process, also causes the waste to available resources in the Fructus Arctii, even causes environmental pollution because of the wasting of resources.Therefore, how could be in the course of processing of Fructus Arctii, the problem of the effective active composition of Fructus Arctii being carried out comprehensive process, classification extraction and application has caused people's attention.
Summary of the invention
At the deficiency of art methods, it is the method for the full component of its water solublity of feedstock production, quintessence oil and oligofructose with cultivation and/or wild Radix Arctii that the technical problem to be solved in the present invention provides a kind of.
A kind of technical scheme of extracting the method for Fructus Arctii active component of the present invention comprises the following steps:
(1) the raw material Radix Arctii is cleaned peeling, cut into slices, dry, use 10W ultrasonic Treatment 10 minutes.
(2) above-mentioned raw materials is added in the reactor, reactor fills N
2, and add an amount of protective agent and hot water, and the water flowing steam distills, and distillation time is 3 to 12 hours, makes the Fructus Arctii quintessence oil.
(3) component of removing quintessence oil that will go up in the step filters with hot water gradation lixiviate, to remove the liquid filtering in the component of quintessence oil exactly, the a certain proportion of 50-90 of solid reuse ℃ of hot water decompression repeats to extract the 1h after-filtration, and filtering lixiviating solution is merged, and carries out concentrating under reduced pressure.
(4) with step (3) gained concentrated solution spray drying, make the full component extract of Fructus Arctii water solublity; Perhaps step (3) gained concentrated solution being added edible ethanol to concentration of alcohol is 60-80%, standing over night, filter pressing, after the gained solid matter adds 60-90 ℃ an amount of hot water and redissolves, add edible ethanol to concentration of alcohol 60-80%, filter pressing, with the solids vacuum drying, obtain burdock fructooligosaccharidcan.
The described steam of step (2) is the steam that high-pressure boiler produces, 110 ℃ of temperature.
The described protective agent of step (2) is selected from the antioxidant content vitamin C, chlorogenic acid, and one or more in the citric acid, additional proportion are 1-10/100000.Add protective agent and can prevent the oxidized destruction of Fructus Arctii active component, the described N that fills
2Be to use N
2Air in the replacement extraction jar also is to destroy for the oxidation that reduces active component.
The described hot water of step (3) is to keep homothermic drinking public water supply; The hot water gradation is extracted 1--2 time; The weight ratio of described raw material and hot water consumption is: fresh burdock root/hot water=1/3-6.
The pressure of the described decompression of step (3) maintains-0.5--0.7mPa, and extracting solution is concentrated into the 1/3-1/5 of original volume.
The described filtration of step (3) is to carry out filter pressing with plate type pressure filter, collects filtrate.
The described spray drying condition of step (4) is that concentrated solution is carried out spray drying at 100 ℃-120 ℃.
The used edible ethanol concentration of the described ethanol precipitation of step (4) is 95%; It is distilled water that described hot water redissolves solids institute water, and temperature is 70-80 ℃, consumption be the solid matter volume 3-5 doubly.
The Fructus Arctii quintessence oil is a contained volatile ingredient in the Fructus Arctii that goes out of extraction separation, identifies 23 kinds of materials altogether, and wherein aldehyde compound is 4 kinds, 3 kinds of terpenoids, 7 kinds of alkane, alkynes vinyl compounds, 8 kinds of organic acid, ester compounds have hair growth promoting, antiinflammatory, the anti-ageing effect of waiting for a long time.Extract oligofructose content reaches 95%, can be used as functional food, feed additive and plant disease resistance inductor.The full component extract of Fructus Arctii water solublity can be used as functional food.Market prospect is wide.
The present invention carries out ultrasonic Treatment to raw material can make the better stripping of active component in the tissue.The present invention provides a kind of rapid and simple method for the contained active component of extraction separation Fructus Arctii, and technology is simple.
The specific embodiment
The method of the invention concrete operations are as follows:
The raw material Radix Arctii is cleaned peeling, cut into slices, dry, use 10W ultrasonic Treatment 10 minutes.The Fructus Arctii sheet that supersound process is crossed adds in the reactor, and reactor fills N
2, and add protective agent, add an amount of hot water in reactor with 110 ℃ of vapor distillations 3 to 12 hours, the acquisition volatile essential oil.Remove the liquid filtering in the component of quintessence oil, a certain proportion of 50-90 of solid reuse ℃ of hot water decompression repeats to extract the 1h after-filtration, filtering lixiviating solution merged carry out concentrating under reduced pressure, and negative pressure maintains-0.5--0.7mPa, is concentrated into the 1/3-1/5 of original volume.
Above-mentioned concentrated solution carries out spray drying at 100 ℃-120 ℃, gets finished product.After measured, oligofructose content about 93% in the finished product, protein about 4%, and alkaloid about 0.05%, aldehydes matter about 0.5%, solvent components about 1%.
It is 65-80% that above-mentioned concentrated solution is regulated concentration of alcohol with 95% edible ethanol, make the burdock fructooligosaccharidcan precipitation, standing over night, remove supernatant, filter pressing, 3-5 times of 70-80 ℃ of distilled water of solid matter reuse redissolves, add ethanol and obtain precipitation, remove supernatant, the filter pressing deposit, vacuum drying obtains burdock fructooligosaccharidcan.Analyze through HP-GPC, UV, IR, SephedexG-50 chromatography, show that above-mentioned Fructus Arctii water solubility extract main component is an oligofructose, the degree of polymerization is 10～17, molar mass average is about 2500, and outward appearance is the loose shape solid of canescence, easily moisture absorption, easily caking is little sweet, odorless.Has peat-reek.Can be dissolved in the cold water, can very fast dissolving in hot water.
Embodiment 1:
As stated above, fresh Radix Arctii is cleaned, drench and do, section.With 10W ultrasonic Treatment 10 minutes.To Fructus Arctii sheet vapor distillation 4h, obtain volatile oil with the hot water of 5 times of volumes.The liquid part is then carried out filter pressing.Raw material repeats to extract once, extraction time 1h, and negative pressure maintains-0.5--0.7mPa, and extracting temperature is 70 ℃, after the filter pressing, merge extractive liquid,, concentrating under reduced pressure, 120 ℃ of spray dryinges get finished product.It is 65% that above-mentioned concentrate is regulated concentration of alcohol with 95% edible ethanol, makes the burdock fructooligosaccharidcan precipitation, and standing over night is removed supernatant, filter pressing, 3 times of 80 ℃ of distilled water of solid matter reuse redissolve ethanol precipitation, remove supernatant, filter pressing, drying obtains burdock fructooligosaccharidcan.
Analyze and standard control through HP-GPC, UV, IR, SephedexG-100 chromatography, show that above-mentioned Fructus Arctii extract is an inulin type oligosaccharide, content 96%, molar mass average are about 2500, and outward appearance is the loose shape solid of canescence, easily moisture absorption, easily caking.Precipitate with ethanol obtains burdock fructooligosaccharidcan content 95%, molecular weight about 2500, no sweet taste.
Embodiment 2:
As stated above, fresh Radix Arctii is cleaned, drench and do, section.With 10W ultrasonic Treatment 10 minutes.To Fructus Arctii sheet vapor distillation 3h, obtain volatile oil with the hot water of 4 times of volumes.The liquid part is then carried out filter pressing.Raw material repeats to extract once, extraction time 1h, and negative pressure maintains-0.5--0.7mPa, and extracting temperature is 80 ℃.After the filter pressing, merge extractive liquid,, concentrating under reduced pressure, 120 ℃ of spray dryinges get finished product.It is 75% that above-mentioned concentrate is regulated concentration of alcohol with 95% edible ethanol, makes the burdock fructooligosaccharidcan precipitation, and standing over night is removed supernatant, filter pressing, 4 times of 75 ℃ of distilled water of solid matter reuse redissolve ethanol precipitation, remove supernatant, filter pressing, drying obtains burdock fructooligosaccharidcan.
Analyze and standard control through HP-GPC, UV, IR, SephedexG-50 chromatography, show that above-mentioned Fructus Arctii extract is an inulin type oligosaccharide, content 93%, molar mass average are about 2500, and outward appearance is the loose shape solid of white light yellow complexion, easily moisture absorption, easily caking.Precipitate with ethanol obtains burdock fructooligosaccharidcan content 95%, molecular weight about 2500, no sweet taste.
Embodiment 3:
As stated above, fresh Radix Arctii is cleaned, drench and do, section.With 10W ultrasonic Treatment 10 minutes.To Fructus Arctii sheet vapor distillation 5h, obtain volatile oil with the hot water of 3 times of volumes.Obtain the liquid part and then carry out filter pressing.Raw material repeats to extract once, extraction time 1h, and negative pressure maintains-0.5--0.7mPa, and extracting temperature is 80 ℃.After the filter pressing, merge extractive liquid,, concentrating under reduced pressure, 120 ℃ of spray dryinges get finished product.It is 70% that above-mentioned concentrate is regulated concentration of alcohol with 95% edible ethanol, makes the burdock fructooligosaccharidcan precipitation, and standing over night is removed supernatant, filter pressing, 5 times of 70 ℃ of distilled water of solid matter reuse redissolve ethanol precipitation, remove supernatant, filter pressing, drying obtains burdock fructooligosaccharidcan.
Analyze and standard control through HP-GPC, UV, IR, SephedexG-100 chromatography, show that above-mentioned Fructus Arctii extract is an inulin type oligosaccharide, content 93%, molar mass average are about 2500, and outward appearance is the loose shape solid of canescence, easily moisture absorption, easily caking.Precipitate with ethanol obtains burdock fructooligosaccharidcan content 95%, molecular weight about 2500, no sweet taste.
Embodiment 4:
As stated above, fresh Radix Arctii is cleaned, drench and do, section.With 10W ultrasonic Treatment 10 minutes.To Fructus Arctii sheet vapor distillation 12h, obtain volatile oil with the hot water of 4 times of volumes.The liquid part is then carried out filter pressing.Raw material repeats to extract once, extraction time 1h, and negative pressure maintains-0.5--0.7mPa, and extracting temperature is 80 ℃.After the filter pressing, merge extractive liquid,, concentrating under reduced pressure, 105 ℃ of spray dryinges get finished product.
Analyze and standard control through HP-GPC, UV, IR, SephedexG-100 chromatography, show that above-mentioned Fructus Arctii extract is an inulin type oligosaccharide, content 95%, molar mass average are about 2500, and outward appearance is the loose shape solid of canescence, easily moisture absorption, easily caking.
Claims (8)
1. method of extracting the Fructus Arctii active component, it comprises the following steps:
(1) the raw material Radix Arctii is cleaned peeling, cut into slices, dry, use 10W ultrasonic Treatment 10 minutes;
(2) above-mentioned raw materials is added in the reactor, reactor fills N
2, and add an amount of protective agent and hot water, and the water flowing steam distills, and distillation time is 3 to 12 hours, makes the Fructus Arctii quintessence oil;
(3) component of removing quintessence oil that will go up in the step filters with hot water gradation lixiviate, be about to remove the liquid filtering in the component of quintessence oil, the a certain proportion of 50-90 of solid reuse ℃ of hot water decompression repeats to extract the 1h after-filtration, and filtering lixiviating solution is merged, and carries out concentrating under reduced pressure;
(4) with step (3) gained concentrated solution spray drying, make the full component extract of Fructus Arctii water solublity; Perhaps step (3) gained concentrated solution being added edible ethanol adjustment concentration of alcohol is 60-80%, standing over night, filter pressing, after the gained solid matter adds 60-90 ℃ an amount of hot water and redissolves, adding edible ethanol to concentration of alcohol is 60-80%, filter pressing with the solids vacuum drying, makes burdock fructooligosaccharidcan.
2. method according to claim 1 is characterized in that: the described steam of step (2) is the steam that high-pressure boiler produces, 110 ℃ of temperature.
3. method according to claim 2 is characterized in that: the described protective agent of step (2) is selected from the antioxidant content vitamin C, chlorogenic acid, and one or more in the citric acid, additional proportion are 1-10/100000.
4. method according to claim 3 is characterized in that: the described hot water of step (3) is to keep homothermic drinking public water supply; It is to use hot water extraction 1--2 time that described hot water gradation is extracted; The weight ratio of described raw material and hot water consumption is: fresh burdock root/hot water=1/3-6.
5. method according to claim 4 is characterized in that: the pressure of the described decompression of step (3) maintains-0.5--0.7mPa, and extracting solution is concentrated into the 1/3-1/5 of original volume.
6. method according to claim 5 is characterized in that: the described filtration of step (3) is to carry out filter pressing with plate type pressure filter, collects filtrate.
7. method according to claim 6 is characterized in that: the described spray drying condition of step (4) is that concentrated solution is carried out spray drying at 100 ℃-120 ℃.
8. method according to claim 7 is characterized in that: the concentration of the described edible ethanol of step (4) is 95%; Described redissolution solids water is a distilled water, and temperature is 70-80 ℃, and consumption is 3-5 a times of solid matter volume.
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| CNA2007101128712A CN101129433A (en) | 2007-09-12 | 2007-09-12 | Method for extracting active component of edible burdock |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526276A (en) * | 2012-01-11 | 2012-07-04 | 张继述 | Anti-hypertension medicinal composition and preparation method for same |
| CN103859578A (en) * | 2014-03-04 | 2014-06-18 | 江苏中烟工业有限责任公司 | Extracting method of burdock crude polysaccharide and application of burdock crude polysaccharide to tobacco |
| CN104172192A (en) * | 2014-08-20 | 2014-12-03 | 博德生物技术（北京）有限公司 | Burdock-oligosaccharide-containing health food with function of cleaning blood |
| CN106176468A (en) * | 2016-08-31 | 2016-12-07 | 百朗德生物化学(海门)有限公司 | A kind of antipollution cosmetic composition and preparation method thereof |
| CN106306118A (en) * | 2015-06-15 | 2017-01-11 | 财团法人食品工业发展研究所 | Preparation method of blend oil containing fat-soluble functional component |
| CN107129541A (en) * | 2017-06-13 | 2017-09-05 | 博德生物技术（德州）有限公司 | A kind of preparation method and applications of burdock polysaccharide pyrogen |
| CN107699353A (en) * | 2017-09-29 | 2018-02-16 | 晨光生物科技集团股份有限公司 | A kind of continuous distillation process |
| CN107987974A (en) * | 2017-12-20 | 2018-05-04 | 安徽省旌德博仕达农业科技有限公司 | A kind of extracting method of sealwort volatile oil |
| CN114307218A (en) * | 2022-01-05 | 2022-04-12 | 吉安市中香天然植物有限公司 | Continuous rectification production control system for spices |
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- 2007-09-12 CN CNA2007101128712A patent/CN101129433A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526276A (en) * | 2012-01-11 | 2012-07-04 | 张继述 | Anti-hypertension medicinal composition and preparation method for same |
| CN103859578A (en) * | 2014-03-04 | 2014-06-18 | 江苏中烟工业有限责任公司 | Extracting method of burdock crude polysaccharide and application of burdock crude polysaccharide to tobacco |
| CN103859578B (en) * | 2014-03-04 | 2016-05-18 | 江苏中烟工业有限责任公司 | The extracting method of the thick polysaccharide of a kind of burdock and the application in tobacco thereof |
| CN104172192A (en) * | 2014-08-20 | 2014-12-03 | 博德生物技术（北京）有限公司 | Burdock-oligosaccharide-containing health food with function of cleaning blood |
| CN106306118A (en) * | 2015-06-15 | 2017-01-11 | 财团法人食品工业发展研究所 | Preparation method of blend oil containing fat-soluble functional component |
| CN106176468A (en) * | 2016-08-31 | 2016-12-07 | 百朗德生物化学(海门)有限公司 | A kind of antipollution cosmetic composition and preparation method thereof |
| CN107129541A (en) * | 2017-06-13 | 2017-09-05 | 博德生物技术（德州）有限公司 | A kind of preparation method and applications of burdock polysaccharide pyrogen |
| CN107699353A (en) * | 2017-09-29 | 2018-02-16 | 晨光生物科技集团股份有限公司 | A kind of continuous distillation process |
| CN107699353B (en) * | 2017-09-29 | 2020-07-03 | 晨光生物科技集团股份有限公司 | Continuous distillation process |
| CN107987974A (en) * | 2017-12-20 | 2018-05-04 | 安徽省旌德博仕达农业科技有限公司 | A kind of extracting method of sealwort volatile oil |
| CN114307218A (en) * | 2022-01-05 | 2022-04-12 | 吉安市中香天然植物有限公司 | Continuous rectification production control system for spices |
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