CN101121945A - Special RAPD molecule marker for wheat 6VS - Google Patents
Special RAPD molecule marker for wheat 6VS Download PDFInfo
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- CN101121945A CN101121945A CNA2007100577790A CN200710057779A CN101121945A CN 101121945 A CN101121945 A CN 101121945A CN A2007100577790 A CNA2007100577790 A CN A2007100577790A CN 200710057779 A CN200710057779 A CN 200710057779A CN 101121945 A CN101121945 A CN 101121945A
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Abstract
The invention discloses a wheat 6VS specific RAPD molecular marker OPK08910 and the nucleotide base sequence of the marker. The marker adopts RAPD molecular labeling technique, phenol-chloroform method is adopted to extract the DNAs of substitution line (6A/6V), translocation line (6AL/6VS), translocation line (6DL/6VS) and Dasypyrum (VV) bearing Dasypyrum V-chromosome and common wheat (AABBDD) and durum wheat (AABB) seedlings without Dasypyrum V-chromosome; 100 10-base random primers are used to screen the obligate and specific segments of 6VS chromosome, and a specific band with a molecular weight about 910bp is expanded by primer OPK08, existing in all the 4 materials containing 6V chromosome and 6VS. Because the specific band has concurrence in two 6VS translocation lines, OPK08910 is the specific molecular marker on 6VS and can be used as an auxiliary option for breeding of wheat resisting powdery mildew.
Description
Technical field
The invention belongs to the agro-biological engineering technical field, particularly wheat 6VS specificity RAPD molecule marker primer, it is as the assisted Selection of wheat powdery mildew breeding for disease resistance molecule marker.The present invention also relates to the preparation method of this specificity RAPD molecule marker primer simultaneously.
Background technology
The importing of allogeneic heredity material particularly has the importing of external source goal gene karyomit(e) or chromosome segment, and the improvement of crop is had crucial effect.The requirement of aspects such as at first human output to raise crop, quality, resistance is more and more higher, next is that original genetic resources can not satisfy the needs of species self or reuses for a long time and cause the height of proterties to degenerate in the species, and wherein wheat is exactly a typical example to the resistance of Powdery Mildew.
Liu Dajun, Chen Peidu etc. import cultivated wheat with the 6V karyomit(e) of cluster hair wheat, breed the 6V substitution line, select 6VS translocation line (Qi Lili, Chen Peidu, Liu Dajun from 6V substitution line and agronomy parent's hybridization and radiation offspring.Acta Agronomica Sinica, 1995,21:257-262).Studies show that, and the Pm21 gene that the strongest, the anti-spectrum of resistance of the last existence of 6VS is the widest (Liu Jinyuan, inscription on pottery is quiet, Liu Dajun. Botany Gazette, 1999,41, (10): 1058-1060; Qi Lili, Chen Peidu, Liu Dajun.Acta Agronomica Sinica, 1995,21:257-262; Chen P D, Qi L L, Zhou B, Zhang Z S, Liu D J.Theor Appl Genet, 1995,91:11115-1128).During reduction division, because the 6AS of 6VS and wheat is unpaired, can not exchange phenomenon, thereby the Pm21 gene follow always the 6AL/6VS translocation chromosome transmit (Liu Dajun, Qi Lili, Chen Peidu. Acta Genetica Sinica, 1996,23 (1): 18-23), therefore seek the 6VS molecule marker, promote that wheat anti-powdery mildew breeding work meaning is very great to making full use of the anti-source of Pm21.
Summary of the invention
The object of the present invention is to provide wheat 6VS obligate molecule marker primer OPK08
910And the nucleotide sequence of this labeled primer.
Another object of the present invention is to provide PCR-based, special RAPD molecule marker for wheat 6 VS primer OPK08
910Preparation method.
Go up the specificity molecule marker in order to obtain wheat 6VS, the present invention is to be contrast not have the chromosomal common wheat of V capital 411 (AABBDD) and a durum wheat (AABB), contain the chromosomal cluster hair wheat of 6V (VV), substitution line (6A/6V), translocation line (6AL/6VS, 6DL/6VS) be material, with randomly amplified polymorphic DNA (RAPD) analytical procedure, above-mentioned materials is carried out primer screening, primer OPK08 shows the polymorphism of amplified production in above-mentioned materials, obtain the RAPD mark OPK08 relevant with 6VS
910This special band is present in all 4 parts of materials that contain 6V karyomit(e) and 6VS, also be present in the translocation line of 2 6VS simultaneously, therefore be 6VS specificity molecule marker, can be used for the wheat powdery mildew assisted selection, this is operated in wheat breeding practice and disease-resistant very high researching value is arranged in theory, and the inventor has finished the present invention just under the guidance of above-mentioned thought.
Technical scheme of the present invention is as follows:
A kind of special RAPD molecule marker for wheat 6 VS is characterized in that described molecule marker OPK08
910Primer adopts following method to obtain with the RAPD molecular marking technique: have the chromosomal substitution line of cluster hair wheat V (6A/6V) with the extraction of phenol one chloroform method, translocation line (6AL/6VS), translocation line (6DL/6VS), cluster hair wheat (VV) and be not with the chromosomal common cultivation wheat of cluster hair wheat V capital 411 (AABBDD) and durum wheat (AABB) seedling DNA, adopt the RAPD molecular marking technique, with 100 10 base random primers, carry out screening with wheat 6VS karyomit(e) obligate specific fragment, find that primer OPK08 amplifies the special band that a molecular weight is about 910bp, be present in all 4 parts of materials that contain 6V karyomit(e) and 6VS, because it is present in two 6VS translocation lines simultaneously, so OPK08
910It is the specificity molecule marker primer on the 6VS.
RAPD primer sequence of the present invention is: 5 ' GAACACTGGG3 ', give birth to worker bio-engineering corporation by Shanghai and synthesize.
Special RAPD molecule marker for wheat 6 VS of the present invention, wherein said OPK08
910The pcr amplification reaction system be: 20ng/ μ l wheat cdna group DNA1 μ l, 10 * PCR Buffer, 2.5 μ l, 25mM MgCl
22.5 μ l, 10mM dNTP 0.5 μ l, 50 μ M primers, 0.5 μ l, 5U/ μ l Taq archaeal dna polymerase 0.25 μ l, ddH
2O 17.75 μ l, total system 25 μ l, response procedures: 1 minute, 35 ℃ annealing of 96 ℃ of sex change are extended 2 minutes, 4 circulations for 1 minute, 72 ℃, and 45 seconds, 36 ℃ annealing of 94 ℃ of sex change are extended 90 seconds, 45 circulations for 1 minute, 72 ℃, 72 ℃ of polishings 10 minutes; Product separates at 1.0% the agarose gel electrophoresis that contains 0.5 μ g/ μ l EB, and ultraviolet lamp is observed down and the photographic recording result.
Wheat 6VS obligate molecule marker OPK08 of the present invention
910, further can obtain by following preparation method:
(1) be contrast not have chromosomal common wheat of V (AABBDD) and a durum wheat (AABB), with contain the chromosomal cluster hair wheat of 6V (VV), substitution line (6A/6V), (6AL/6VS 6DL/6VS) is material to translocation line;
(2) extract above-mentioned wheat parent seedling and filial generation seedling DNA with phenol-chloroform method; Detect the concentration of DNA sample respectively with ultraviolet spectrophotometry, 0.7% agarose gel electrophoresis detects the integrity of DNA.
(3) adopt the RAPD molecule marking method to carry out the screening of special RAPD molecule marker for wheat 6 VS; Filter out 1 RAPD molecule marker OPK08
910, the primer base sequence that wherein obtains this mark is: GAACACTGGG;
(4) with OPK08
910Clone and order-checking.Entrust Shanghai to give birth to the order-checking of worker bio-engineering corporation, find OPK08
910Actual (tube) length 910bp.
Special RAPD molecule marker for wheat 6 VS OPK08 of the present invention
910Base sequence be:
gaacactgggacctcgtggcacagcaagtcctctggcacttgggactccaaagaccccctcatggcggctta
taggcgagaggtggatgctatcgcagctcacttcaatggagatcatgtggaccggaggaagaaggcagctg
atgctttaagccggctgggatctcaaacaacgcctcagatgcaggtacgacagagtttggataatcacgctgg
ggacgctcaggagctgcagtagtatgacgaggaggccgtttctatgttacctatccggacgatcagtagttgg
gattctaggctgacaagggagatccgggtgcagttggtggttatctttctagatccggagtcggataactatgag
attgtaataattattgctataatgctatattatattctggggcatgtgtttgcctggctttgtacttctgtatcctttcttat
gttgtatgcgtgtatgattatcccctctgattgatcgaaccgtttgaacttgctttactgttgactgcaggtggttgat
gctttcgtgccggagttctagaaaagttttgagtccttagtttcacgtcactcttatatcatctcagttccgaatacca
cggccgactgaggatcgaaaataggaatcacgcgggtattttcgatgatctggtatgatgtttatgtgacggga
cctctgtctcaaaattagccccagtagggcgtggtggtccacgcctgtaatcccagtgactcaggaggctgag
gcaggagaatcacctgaatccgggaggcagatgcagtgagccaagactgcgctactgcattccagcctggg
agacagagtgcagactccgtccagacaacatagcagaggccacagctgcagaaggacccaggtgaggaca
ggagtac
cccagtgttc。
(6AL/6VS 6DL/6VS) has cluster hair wheat 6V karyomit(e) or the short arm of a chromosome 6VS for wheat cluster dirty wheat chromosome substitution system (6A/6V), translocation line.Cytogenetical study shows that wheat powdery mildew resistant gene Pm21 is positioned on the cluster hair wheat 6VS chromosome arm.During reduction division, because the 6AS of 6VS and wheat is unpaired, can not exchange phenomenon, thereby the Pm21 gene is followed the transmission of 6AL/6VS translocation chromosome always, the screening of 6V obligate molecule marker is to effective tracking Pm21 mildew-resistance gene, thereby it is significant to be used for the disease-resistant wheat assisted selection.
Special RAPD molecule marker for wheat 6 VS (OPK08 of the present invention
910) compare its advantage and beneficial effect is with underlined:
(1) experiment material of the present invention has salient feature: common wheat capital 411 (AABBDD) and durum wheat (AABB) are not had V karyomit(e), and other is for containing the chromosomal cluster hair wheat of 6V (VV), contain the chromosomal substitution line of 6V (6A/6V), containing the chromosomal translocation line of 6VS (6AL/6VS) and another contains the chromosomal translocation line of 6VS (6DL/6VS).Do not have in the chromosomal material of V but in the chromosomal material of tool V, all have and containing the special band that has in the chromosomal material of 6VS as long as we find not having in the experimentation, just can judge that this special band is a 6VS obligate mark.
(2) the used experimental technique of the present invention is simple, carries out the screening of specific mark with the RAPD method, has that material usage is few, the result detects easily, characteristics easy and simple to handle.
(3) the present invention obtains 6VS specificity molecule marker OPK08 with the RAPD molecular marking technique
910Primer is effectively to follow the tracks of the chromosomal transmission of 6VS in the breeding process, and then follows the tracks of powdery mildew resistance gene Pm 21 reliable basis is provided, and more likely is used for production practice.
Description of drawings
Fig. 1: genome dna electrophoresis figure;
Fig. 2: RAPD screens figure;
Fig. 1 wherein, the symbol that is occurred in 2 is expressed as respectively:
1: common wheat capital 411 (AABBDD);
2: durum wheat (AABB);
3: cluster hair wheat (VV);
4: substitution line (6A/6V);
5: translocation line (6AL/6VS);
6: translocation line (6DL/6VS)
M：λDNA?Hind?III/EcoR?I?Marker。
Embodiment: (specifying in conjunction with the accompanying drawings)
In order to explain enforcement of the present invention more fully, provide following special RAPD molecule marker for wheat 6 VS (OPK08
910) the preparation embodiment.These embodiments only are to explain rather than limit the scope of the invention.
Wherein common wheat capital 411 (AABBDD), durum wheat (AABB) are public, are externally provided by China Agricultural University's agronomy and biotechnology institute.Wheat-haynaldia villosa (VV), substitution line (6A/6V) and contain the chromosomal translocation line of 6VS (6AL/6VS) and another contains the chromosomal translocation line of 6VS (6DL/6VS) and is externally provided by China Agricultural University's agronomy and biotechnology institute for public.The RAPD primer sequence is: 5 ' GAACACTGGG3 ', give birth to worker bio-engineering corporation by Shanghai and synthesize.
The separation of genomic dna:
Get 0.2g etiolated seedling blade, in liquid nitrogen, grind, be powder as far as possible, add rapidly 2 milliliters extract Buffer (50mM Tris-HCl, pH 8.0; 20mM EDTA; 50mM NaCl), mix gently, 65 ℃ of temperature are bathed 15min, put upside down mixing 2-3 time therebetween.0 ℃ of ice bath 10 minutes, 0-4 ℃, 3000 rev/mins centrifugal 15 minutes, beat easily supernatant liquor, join in the new pipe, add the saturated phenol of equal-volume Tris-HCl (pH 8.0), mix gently.0-4 ℃, 3000 rev/mins centrifugal 10 minutes, get supernatant liquor, add equal-volume phenol, chloroform-primary isoamyl alcohol (25: 24: 1) extracting, mixing gently.0-4 ℃, 3000 rev/mins centrifugal 10 minutes, get supernatant liquor, add equal-volume chloroform-primary isoamyl alcohol (25: 24: 1) extracting, gently mixing.0-4 ℃, 3000 rev/mins, centrifugal 10 minutes, get supernatant liquor, add 2 times of cold ethanol of volume ,-20 ℃ of precipitations are more than 1 hour.0-4 ℃, 12000 rev/mins, centrifugal 20 minutes, abandon supernatant liquor, 75% ethanol 0-4 ℃ is of short duration centrifugal, washes precipitation 2 times, dries, and is dissolved in 100 μ l TE (pH8.0).Add 1 μ l RNase (10mg/ml), 37 ℃ are incubated 1 hour, to remove the RNA in the sample.Add equal-volume phenol, chloroform, extracting once, 0-4 ℃, 3000 rev/mins are centrifugal 10 minutes.Get supernatant liquor, add the extracting of equal-volume chloroform once, 0-4 ℃, 3000 rev/mins centrifugal 10 minutes.Get supernatant liquor, add the cold ethanol of two volumes ,-20 ℃ of precipitations are more than 2 hours.0-4 ℃, 12000 rev/mins, centrifugal 20 minutes.Abandon supernatant liquor, 75% ethanol 0-4 ℃ of short duration centrifugal, washes precipitation 2 times, dries, and is dissolved among the 50 μ l TE (pH8.0).Detect the concentration of DNA sample respectively with ultraviolet spectrophotometry, 0.7% agarose gel electrophoresis detects the integrity (see figure 1) of DNA.
The screening of molecule marker:
(1) utilize 100 random primers (10bp) at common wheat capital 411 (AABBDD), durum wheat (AABB), cluster hair wheat (VV), substitution line (6A/6V) with contain the chromosomal translocation line of 6VS (6AL/6VS) and another contains between the chromosomal translocation line of 6VS (6DL/6VS) and carries out the dna polymorphism analysis.
(2) on the PE-9700PCR instrument, be that primer sequence carries out pcr amplification with 5 ' GAACACTGGG3 ' PCR (10bp).Reaction system is: 20ng/ μ l wheat cdna group DNA1 μ l, 10 * PCRBuffer2.5 μ l, 25mM MgCl
22.5 μ l, 10mM dNTP0.5 μ l, 50 μ M primers, 0.5 μ l, 5U/ μ l Taq archaeal dna polymerase 0.25 μ l, ddH2O 17.75 μ l, total system 25 μ l,
(3) response procedures: 1 minute, 35 ℃ annealing of 96 ℃ of sex change are extended 2 minutes, 4 circulations for 1 minute, 72 ℃, and 45 seconds, 36 ℃ annealing of 94 ℃ of sex change are extended 90 seconds, 45 circulations for 1 minute, 72 ℃, 72 ℃ of polishings 10 minutes.
(4) product detects: containing 1.0% the agarose gel electrophoresis of 0.5 μ g/ μ l EB, ultraviolet lamp is observed down and the photographic recording result.
OPK08
910The acquisition of mark:
In above-mentioned 100 random primers, primer OPK08 shows the polymorphism of amplified production, be the primer OPK08 band that molecular weight is 910bp that increases in containing the chromosomal wheat of V karyomit(e) and 6VS, this specific band (see figure 2) does not increase in not containing the chromosomal common wheat of V capital 411 and durum wheat.This special band is present in all 4 parts of materials that contain 6V karyomit(e) and 6VS, also is present in the translocation line of 2 6VS simultaneously.Therefore, experimental result shows: OPK08
910It is 6VS specificity molecule marker.
OPK08
910Clone and order-checking:
Mainly consult pGEM-T easy Vector System (Promega company) specification sheets, add following composition reaction: 2 * Buffer 5 μ l that connect in the following order on ice, pGEM-T easyvector (50mg/ml) 1 μ l, the PCR product 2 μ l that add the A tail, T4 ligase enzyme (3U/ μ l) 1 μ l, aseptic two H that steam
2O polishing 10 μ l; Pressure-vaccum mixing gently, 4 ℃ of connections are spent the night.To connect product 2 μ l and be transformed among the 100 μ l competent cell DH5 α, filter out positive colony, and entrust Shanghai to give birth to the order-checking of worker bio-engineering corporation, result: OPK08
910Actual (tube) length 910bp sees OPK08
910The nucleotides sequence tabulation.
After the preferred embodiment that describes in detail, the personage who is familiar with this technical field can be well understood to, can carry out various forms of variations and modification not breaking away under above-mentioned claim and the spirit, all foundations technical spirit of the present invention all belongs to the scope of technical solution of the present invention to any simple modification, equivalent variations and modification that above embodiment did.And the embodiment that the present invention also is not subject in the specification sheets to be given an actual example.
SEQUENCE?LISTING
＜110〉Tianjin Normal University
＜120〉special RAPD molecule marker for wheat 6 VS
<130>000
<160>2
<170>PatentIn?version?3.2
<210>1
<211>910
<212>DNA
＜213〉cluster hair wheat＜Haynaldia villosal 〉
<220>
<221>gene
<222>(1)..(910)
<400>1
gaacactggg?acctcgtggc?acagcaagtc?ctctggcact?tgggactcca?aagaccccct 60
catggcggct?tataggcgag?aggtggatgc?tatcgcagct?cacttcaatg?gagatcatgt 120
ggaccggagg?aagaaggcag?ctgatgcttt?aagccggctg?ggatctcaaa?caacgcctca 180
gatgcaggta?cgacagagtt?tggataatca?cgctggggac?gctcaggagc?tgcagtagta 240
tgacgaggag?gccgtttcta?tgttacctat?ccggacgatc?agtagttggg?attctaggct 300
gacaagggag?atccgggtgc?agttggtggt?tatctttcta?gatccggagt?cggataacta 360
tgagattgta?ataattattg?ctataatgct?atattatatt?ctggggcatg?tgtttgcctg 420
gctttgtact?tctgtatcct?ttcttatgtt?gtatgcgtgt?atgattatcc?cctctgattg 480
atcgaaccgt?ttgaacttgc?tttactgttg?actgcaggtg?gttgatgctt?tcgtgccgga 540
gttctagaaa?agttttgagt?ccttagtttc?acgtcactct?tatatcatct?cagttccgaa 600
taccacggcc?gactgaggat?cgaaaatagg?aatcacgcgg?gtattttcga?tgatctggta 660
tgatgtttat?gtgacgggac?ctctgtctca?aaattagccc?cagtagggcg?tggtggtcca 720
cgcctgtaat?cccagtgact?caggaggctg?aggcaggaga?atcacctgaa?tccgggaggc 780
agatgcagtg?agccaagact?gcgctactgc?attccagcct?gggagacaga?gtgcagactc 840
cgtccagaca?acatagcaga?ggccacagct?gcagaaggac?ccaggtgagg?acaggagtac
900
cccagtgttc
910
<210>2
<211>10
<212>DNA
＜213〉artificial sequence
<400>2
gaacataggg
10
Claims (3)
1. special RAPD molecule marker for wheat 6 VS is characterized in that described molecule marker OPK08
910Adopt the RAPD molecular marking technique to obtain: at first to have the chromosomal substitution line of cluster hair wheat V (6A/6V) with phenol-chloroform method extraction, translocation line (6AL/6VS), translocation line (6DL/6VS), cluster hair wheat (VV) and be not with the chromosomal common cultivation wheat of cluster hair wheat V (AABBDD) and durum wheat (AABB) seedling DNA, carry out screening with wheat 6VS karyomit(e) obligate specific fragment with 100 10 base random primers then, find that primer OPK08 amplifier molecule amount is about the special band of 910bp, this special band is present in all 4 parts of materials that contain 6V karyomit(e) and 6VS; Wherein used RAPD primer sequence is: 5 ' GAACACTGGG3 '.
2. special RAPD molecule marker for wheat 6 VS as claimed in claim 1, wherein said OPK08
910The pcr amplification reaction system be: 20ng/ μ l wheat cdna group DNA1 μ l, 10 * PCR Buffer2.5 μ l, 25mM MgCl
22.5 μ l, 10mM dNTP0.5 μ l, 50 μ M primers, 0.5 μ l, 5U/ μ l TaqDNA polysaccharase 0.25 μ l, ddH
2O 17.75 μ l, total system 25 μ l, response procedures: 1 minute, 35 ℃ annealing of 96 ℃ of sex change are extended 2 minutes, 4 circulations for 1 minute, 72 ℃, and 45 seconds, 36 ℃ annealing of 94 ℃ of sex change are extended 90 seconds, 45 circulations for 1 minute, 72 ℃, 72 ℃ of polishings 10 minutes; Product separates at 1.0% the agarose gel electrophoresis that contains 0.5% μ g/ μ l EB, and ultraviolet lamp is observed down and the photographic recording result.
3. special RAPD molecule marker for wheat 6 VS OPK08 according to claim 1
910Base sequence be:
gaacactgggacctcgtggcacagcaagtcctctggcacttgggactccaaagaccccctcatggcggctta
taggcgagaggtggatgctatcgcagctcacttcaatggagatcatgtggaccggaggaagaaggcagctg
atgctttaagccggctgggatctcaaacaacgcctcagatgcaggtacgacagagtttggataatcacgctgg
ggacgctcaggagctgcagtagtatgacgaggaggccgtttctatgttacctatccggacgatcagtagttgg
gattctaggctgacaagggagatccgggtgcagttggtggttatctttctagatccggagtcggataactatgag
attgtaataattattgctataatgctatattatattctggggcatgtgtttgcctggctttgtacttctgtatcctttcttat
gttgtatgcgtgtatgattatcccctctgattgatcgaaccgtttgaacttgctttactgttgactgcaggtggttgat
gctttcgtgccggagttctagaaaagttttgagtccttagtttcacgtcactcttatatcatctcagttccgaatacca
cggccgactgaggatcgaaaataggaatcacgcgggtattttcgatgatctggtatgatgtttatgtgacggga
cctctgtctcaaaattagccccagtagggcgtggtggtccacgcctgtaatcccagtgactcaggaggctgag
gcaggagaatcacctgaatccgggaggcagatgcagtgagccaagactgcgctactgcattccagcctggg
agacagagtgcagactccgtccagacaacatagcagaggccacagctgcagaaggacccaggtgaggaca
ggagtac
cccagtgttc。
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|---|---|---|---|
| CNA2007100577790A CN101121945A (en) | 2007-06-29 | 2007-06-29 | Special RAPD molecule marker for wheat 6VS |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100577790A CN101121945A (en) | 2007-06-29 | 2007-06-29 | Special RAPD molecule marker for wheat 6VS |
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| CN101121945A true CN101121945A (en) | 2008-02-13 |
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ID=39084443
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103233006A (en) * | 2013-05-10 | 2013-08-07 | 南京农业大学 | Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus |
| CN103421776A (en) * | 2013-08-19 | 2013-12-04 | 中国科学院成都生物研究所 | Genome-specific molecular marker primer of annual diploid dasypyrum villosum and application of primer |
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN105087760A (en) * | 2014-05-07 | 2015-11-25 | 江苏省农业科学院 | Labeled primer of wheat powdery mildew resistance gene Stpk-V and application of labeled primer |
| CN105695589A (en) * | 2016-03-18 | 2016-06-22 | 中国农业科学院作物科学研究所 | Complete set of reagent and molecular marker for detecting containing of 6V#4S chromosome arm of dasypyrum villosum in wheat |
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2007
- 2007-06-29 CN CNA2007100577790A patent/CN101121945A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103233006A (en) * | 2013-05-10 | 2013-08-07 | 南京农业大学 | Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus |
| CN103233006B (en) * | 2013-05-10 | 2014-10-29 | 南京农业大学 | Specific molecular markers of haynaldia villosa 4VS chromosome capable of resisting wheat yellow mosaic virus |
| CN103421776A (en) * | 2013-08-19 | 2013-12-04 | 中国科学院成都生物研究所 | Genome-specific molecular marker primer of annual diploid dasypyrum villosum and application of primer |
| CN103421776B (en) * | 2013-08-19 | 2015-07-15 | 中国科学院成都生物研究所 | Genome-specific molecular marker primer of annual diploid dasypyrum villosum and application of primer |
| CN105087760A (en) * | 2014-05-07 | 2015-11-25 | 江苏省农业科学院 | Labeled primer of wheat powdery mildew resistance gene Stpk-V and application of labeled primer |
| CN105087760B (en) * | 2014-05-07 | 2018-05-01 | 江苏省农业科学院 | The labeled primer of powdery mildew resistance gene in wheat Stpk-V a kind of and application |
| CN104278028A (en) * | 2014-09-16 | 2015-01-14 | 天津师范大学 | Sequence of dasypyrum villosum 6VS DNA permeating into powdery mildew resistant near-isogenic line of wheat and application |
| CN104365471A (en) * | 2014-11-06 | 2015-02-25 | 南京农业大学 | Breeding and identifying method of soft and powdery mildew resistant triticum aestivum-Dasypyrum villosum translocation line |
| CN104365471B (en) * | 2014-11-06 | 2016-06-15 | 南京农业大学 | One soft, the selection-breeding of mildew-resistance Triticum aestivum-Haynaldia villosa translocation line, authentication method |
| CN105695589A (en) * | 2016-03-18 | 2016-06-22 | 中国农业科学院作物科学研究所 | Complete set of reagent and molecular marker for detecting containing of 6V#4S chromosome arm of dasypyrum villosum in wheat |
| CN105695589B (en) * | 2016-03-18 | 2019-03-15 | 中国农业科学院作物科学研究所 | Detect wheat whether reagent set and molecular labeling containing haynaldia villosa 6V#4S chromosome arm |
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