CN101120641A - Seedling budding method of micro-bud strips of rubber tree - Google Patents
Seedling budding method of micro-bud strips of rubber tree Download PDFInfo
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- CN101120641A CN101120641A CNA2006101152473A CN200610115247A CN101120641A CN 101120641 A CN101120641 A CN 101120641A CN A2006101152473 A CNA2006101152473 A CN A2006101152473A CN 200610115247 A CN200610115247 A CN 200610115247A CN 101120641 A CN101120641 A CN 101120641A
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- 238000000034 method Methods 0.000 title claims abstract description 28
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- 229920001971 elastomer Polymers 0.000 claims abstract description 15
- 229930006000 Sucrose Natural products 0.000 claims description 12
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 11
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 9
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- 229960001669 kinetin Drugs 0.000 claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 210000001161 mammalian embryo Anatomy 0.000 claims description 6
- 230000000392 somatic effect Effects 0.000 claims description 6
- 210000002242 embryoid body Anatomy 0.000 claims description 4
- 229930191978 Gibberellin Natural products 0.000 claims description 3
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 3
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 3
- 239000003448 gibberellin Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 claims description 2
- 239000004576 sand Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 235000020415 coconut juice Nutrition 0.000 claims 2
- 230000035784 germination Effects 0.000 claims 1
- 230000000366 juvenile effect Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000004904 shortening Methods 0.000 abstract 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 6
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 5
- 235000017491 Bambusa tulda Nutrition 0.000 description 5
- 241001330002 Bambuseae Species 0.000 description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 5
- 239000011425 bamboo Substances 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 210000001015 abdomen Anatomy 0.000 description 4
- 239000002775 capsule Substances 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000005266 casting Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
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- 238000009395 breeding Methods 0.000 description 2
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- 230000000763 evoking effect Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
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- 239000002689 soil Substances 0.000 description 2
- 239000005972 6-Benzyladenine Substances 0.000 description 1
- 241000221020 Hevea Species 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 229920003211 cis-1,4-polyisoprene Polymers 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明涉及一种用于培育橡胶芽接树的优良芽条、籽苗砧木及其嫁接技术，特别涉及一种橡胶树微型芽条的籽苗芽接方法，本发明所通过花药培养结合离体微繁技术培育的橡胶微型芽条，芽条直径只有0.2厘米，芽眼小，侧芽密集，芽的利用率高。芽接时，芽片的木质部不需剥除，用之与展叶期前橡胶籽苗为砧木进行的芽接，芽接速度快，用这种芽条芽接培育出来的是幼态芽接树，生长更快、产量更高。本发明橡胶树微型芽条的籽苗芽接方法提供的微型芽条进行籽苗芽接所培育的是幼态无性系，不仅达到了缩短橡胶树芽接苗的培育周期的目的，而且比现有橡胶树籽苗芽接培育的老态无性系提高干胶产量10－30％、生长速度加快5％左右，可提早半年开割投产。The present invention relates to an excellent bud strip for cultivating rubber bud grafting trees, seedling rootstock and grafting technology thereof, in particular to a method for seedling bud grafting of rubber tree micro-bud strips, the invention adopts anther culture combined with in vitro micropropagation technology The cultivated rubber micro-bud strips have a diameter of only 0.2 cm, small bud eyes, dense side buds, and high bud utilization. When budding, the xylem of the bud sheet does not need to be stripped off, and it is used for budding with rubber seedlings before the leaf-expansion stage as the rootstock, and the budding speed is fast. The juvenile budding tree grown by budding with this kind of bud strips grows faster , Higher output. The seedling budding method provided by the micro-bud strips of the rubber tree of the present invention is a juvenile clone cultivated by the micro-bud strips for the seedling bud-grafting, which not only achieves the purpose of shortening the cultivation period of the rubber tree bud-grafting seedlings, but also has a better effect than the existing rubber tree seedling budding. The cultivated old clone can increase the dry rubber output by 10-30%, and the growth speed can be accelerated by about 5%, and can be cut and put into production half a year earlier.
Description
Technical field
The present invention relates to a kind of good budling, seedling stock and graft technology thereof that is used to cultivate rubber bud grafting tree, the budding method of particularly a kind of rubber tree minitype budling.
Background technology
Present bamboo grows budding method, the cultivation of budling is to carry out in the field, need bigger land area, in the production practices of bamboo grows budding, in the rubber seedling before the first fluffy true leaf leaf stretching period, bud grafting mouth position is selected on the flat horizontal surface of hypocotylar Lower Half, opening width with budding knife or scalpel is about 1/2 of stem week, be about 3-4 centimetre bud grafting mouth, about 2 centimetres on bud grafting mouth lower end ion leaf keeps about 1.5-2 centimetre abdomen capsule skin, in order to folder holder bud sheet, it is big that the budding method of existing budling is opened the bud grafting mouth, is unfavorable for the healing of bud grafting mouth.When getting the bud sheet, from bottom to up,, do not cut the bud sheet, remove bud sheet xylem, to finishing around the bud sheet, make the bud sheet be equal to or less than the bud grafting mouth then with pushing top process with the bud sheet of petiole.The bud sheet of band petiole is respectively carved a cutter with budding knife around eye, reach xylem deeply, takes out the bud sheet, to finishing around the bud sheet, makes the bud sheet be equal to or less than the bud grafting mouth then, also will repair petiole simultaneously, gets trouble of bud sheet and finishing bud sheet.Because budling is unbecoming with stock, the diameter of seedling stock is about 0.3 centimetre, the budling diameter is 0.5～1.2 centimetre, from diameter is that 0.5～1.2 centimetre of budling is got the bud sheet to receive diameter be on the seedling about 0.3 centimetre, but about experienced operator bud grafting 120 strains in a day, in the bud grafting operating process, there is the problem of getting bud, repairing many inconveniences such as bud.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the budding method of a kind of rubber tree minitype budling is provided, it is little to solve the seedling diameter, and the budling diameter is big, has promptly solved the unbecoming technical barrier that is difficult to bud grafting of budling and stock.
1, in order to achieve the above object, the object of the present invention is achieved like this: the budding method of rubber tree minitype budling, be used to cultivate budling, seedling stock and the graft technology thereof of rubber bud grafting tree, its step comprises: the cultivation of (1), stock, (2), the cultivation of miniature budling, (3), seedling is opened the bud grafting mouth, (4), miniature budling cuts the bud sheet, (5), bud grafting, (6), plant seedling, (7), separate and tie up and chipping, it is characterized in that: the budding method of rubber tree minitype budling, its step:
(1), in the nurturing period of stock, select fresh seeds and on casting bed vernalization 18-22 days, choose the about 20-30 of height of seedling centimetre seedling, in order to bud grafting;
(2), in the nurturing period of miniature budling, be explant with rubber flower pesticide, at the additional 2.4-D 0.5-1mg/l of MS minimal medium, kinetin 0.5-1mg/l, methyl 1-2mg/l, Sucus Cocois 5%, sucrose concentration are evoked callus on the medium of 70-100g/l; At the additional kinetin 0.2-1mg/l of MS minimal medium, methyl 0.1-0.2mg/l, Sucus Cocois 5%, active carbon 1g/l, sucrose concentration are inducing embryoid body on the medium of 70g/l; At the MS60-80% macroelement, double the additional kinetin 0.3-0.5mg/l of trace element, gibberellin 2mg/l, active carbon 1g/l, sucrose concentration induce flower pesticide somatic embryo plant on the medium of 50g/l; Cut lateral bud the flower pesticide somatic embryo plant and terminal bud is an explant from aseptic young age, at the additional 6-benzyl aminoadenine 2-3mg/l of MS minimal medium, methyl 0.1-0.2mg/l, sucrose concentration breed budling on the medium of 50-60g/l;
When (3), seedling is opened bud grafting mouth operation, be selected in before the first fluffy true leaf leaf stretching period of rubber seedling, hypocotylar Lower Half is flat, bud grafting mouth position is selected on the flat horizontal surface of hypocotylar Lower Half, opening width with medical surgical knife is about 1/3 of stem week, is about 1.3-1.5 centimetre bud grafting mouth, 1.5-2.0 centimetre on bud grafting mouth lower end ion leaf, keep about 0.5 centimetre abdomen capsule skin, in order to folder holder bud sheet;
When (4), miniature budling cuts bud sheet operation, 5 centimetres to 10 centimetres high of taking-ups, stem slightly are 0.15-0.25 centimetre from test tube, the budling that leaf is in growth stationary phase or has fallen leaves, cut the bud sheet with the medical operation blade from bottom to up with pushing top process, the xylem of bud sheet needn't be removed, in case of necessity the two ends of bud sheet are repaired a little and get final product, the length of bud sheet is 0.5-0.8 centimetre, whenever cuts a bud sheet and connects a strain immediately.
The beneficial effect of the budding method of rubber tree minitype budling of the present invention is: the miniature budling diameter that 1) the present invention used is littler than stock, carries out the budding service speed with miniature budling and can improve 30-50% than carrying out the budding service speed with existing budling.2) eye of Yun Yong miniature budling is many, budling availability height.3) under isolated condition, the speed of breeding miniature budling is for conventional more than 5 times of budling of breeding of producing, and is bigger to the rapid popularizing action of new cultivation or the new rubber new varieties of introducing.4) present technique provides the miniature budling that a kind of developmental stage is the juvenile form type, what bud grafting produced is the juvenile form clone, not only reached the purpose that shortens the cultivation period of bamboo grows budling, and with carry out the stereotype clone that budding cultivates with existing budling and compare, improve dried glue output 10-30%, growth rate and accelerate about 5%, can do sth. in advance half a year and open to cut operation.
Embodiment
For example the present invention is described in further detail below:
The nurturing period of stock: in the hevea seed mature period, collect fresh seeds and on casting bed vernalization 18-22 days, choose the about 20-30 of height of seedling centimetre and be in the seedling that is stretched to leaf stretching period, seedling is pulled up from casting bed, the sand of seedling basal part of stem and root is removed in the water flushing, and the seedling root is immersed in the container that fills shallow water, in order to bud grafting.
The nurturing period of miniature budling: is explant with rubber flower pesticide earlier, at the additional 2.4-D0.5-1mg/l of MS minimal medium, kinetin 0.5-1mg/l, methyl 1-2mg/l, Sucus Cocois 5%, sucrose concentration are evoked callus on the medium of 70-100g/l; At the additional kinetin 0.2-1mg/l of MS minimal medium, methyl 0.1-0.2mg/l, Sucus Cocois 5%, active carbon 1g/l, sucrose concentration are inducing embryoid body on the medium of 70g/l; At the MS60-80% macroelement, double trace element, additional kinetin 0.3-0.5mg/l, gibberellin 2mg/l, active carbon 1g/l, sucrose concentration are that inducing embryoid body is sprouted into the somatic embryo plant on the medium of 50g/l; Cut lateral bud the flower pesticide somatic embryo plant and terminal bud is an explant from aseptic young age again, at the additional 6-benzyladenine 2-3mg/l of MS minimal medium, methyl 0.1-0.2mg/l, sucrose concentration increase numerous budling on the medium of 50-60g/l.The cultivation of miniature budling is to carry out indoor, be batch production production, just can cultivate a large amount of miniature budlings with less space, miniature budling reproduction speed is for having the used budling reproduction speed of seeds of trees seedling bud grafting method now more than 5 times, this budling eye is many, budling availability height, but the technology that miniature budling is cultivated is complicated.
When seedling is opened bud grafting mouth operation; Before the first fluffy true leaf leaf stretching period of rubber seedling, hypocotylar Lower Half is flat, bud grafting mouth position is selected on the flat horizontal surface of hypocotylar Lower Half, opening width with medical surgical knife is about 1/3 of stem week, be about 1.3-1.5 centimetre bud grafting mouth, 1.5-2.0 centimetre on bud grafting mouth lower end ion leaf keeps about 0.5 centimetre abdomen capsule skin, in order to folder holder bud sheet; Because the budding bud grafting that method is opened of miniature budling is young, helps the healing of bud grafting mouth.
When miniature budling cut bud sheet operation: 5-10 centimetre high of taking-up, stem slightly were 0.15-0.25 centimetre from test tube, the budling that leaf is in growth stationary phase or has fallen leaves, cut the bud sheet with the medical operation blade from bottom to up with pushing top process, the xylem of bud sheet needn't be removed, in case of necessity the two ends of bud sheet are repaired a little and get final product, the length of bud sheet is 0.5-0.8 centimetre, whenever cuts a bud sheet and connects a strain immediately, and is very convenient.
Bud grafting: it is the material material that the plastic film food fresh keeping bag of a piece of wood serving as a brake to halt a carriage is arranged with commercially available softness, is cut into wide 0.8-1.0 centimetre, and the bundling belt that is about 15-20 centimetre is standby.The bud sheet is inserted on the bud grafting mouth, and the lower end of bud sheet by the holder of abdomen capsule leather wallet, bundlees from lower to upper with bundling belt.The seedling that bud grafting is good is re-applied in another container that fills shallow water, in order to planting seedling.The budding method difference of budding method of miniature budling and existing budling is: the former is because the bud sheet is less, and the bud sheet is easy to be attached on the bud grafting mouth, thereby binding is also easy; And the latter is thicker owing to the bud sheet, and the bud sheet is not easy to be attached on the bud grafting mouth, and binding must be very careful, otherwise can cause the bud sheet to drop in the binding process, and get the bud sheet, and finishing bud sheet is difficult.
Plant seedling: plant Miao Qianxian with wide 15 centimetres, it is standby that dark 30 centimetres plastic sack installs nutrition soil, to be a working cell per half a day, seedling plantation before each working cell time will finish that bud grafting is good is in plastic sack nutrition soil, drenched normal root water, later on every day trickle once, and sunshade is about 2 months.
Separate and tie up and chipping: separate after bud grafting 25-30 days and tie up, the seedling that bud grafting survives keeps 2 compound leaves of the first fluffy leaf, and with tender stem or terminal bud excision, after about 2 weeks, chipping below the first fluffy leaf can be sprouted about 15 to 20 days after the chipping above blade.2 compound leaves that also can keep the first fluffy leaf are until the stable chippings not of the back young leaves that sprouts, and the benefit of this method is that the blade of stock can continue as newly to sprout provides nutrient, and it is withered to prevent from newly to sprout back, but the reservation compound leaf, it is slower to sprout.
What the miniature budling that the budding method of rubber tree minitype budling of the present invention provides carried out that budding cultivates is the juvenile form clone, not only reached the purpose that shortens the cultivation period of bamboo grows budling, and than the stereotype clone that existing bamboo grows budding is cultivated improve dried glue output 10-30%, growth rate accelerates about 5%, can do sth. in advance half a year and open to cut operation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006101152473A CN101120641B (en) | 2006-08-11 | 2006-08-11 | Seedling budding method for rubber tree minitype bud sticks |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2006101152473A CN101120641B (en) | 2006-08-11 | 2006-08-11 | Seedling budding method for rubber tree minitype bud sticks |
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| CN101120641A true CN101120641A (en) | 2008-02-13 |
| CN101120641B CN101120641B (en) | 2011-08-10 |
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| CN2006101152473A Expired - Fee Related CN101120641B (en) | 2006-08-11 | 2006-08-11 | Seedling budding method for rubber tree minitype bud sticks |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388801A (en) * | 2011-07-27 | 2012-03-28 | 青岛农业大学 | Sterile grafting method of peanut tissue culture seedlings |
| CN102388735A (en) * | 2011-07-28 | 2012-03-28 | 广东农垦热带作物科学研究所 | Small-sized green budling batch multiplication method for rubber trees |
| CN102696390A (en) * | 2012-07-03 | 2012-10-03 | 广东农垦热带作物科学研究所 | Method for promoting budding of seedling budlings of Brazil rubber trees |
| CN102792856A (en) * | 2012-07-11 | 2012-11-28 | 云南省红河热带农业科学研究所 | Jackfruit juvenile bud grafting method |
| CN102860221A (en) * | 2012-07-14 | 2013-01-09 | 中国热带农业科学院橡胶研究所 | Rubber tree seedling cultivation method |
| CN103125382A (en) * | 2011-12-02 | 2013-06-05 | 云南省热带作物科学研究所 | Micropropagation method for rubber tree good variety somatic embryo plant |
| CN103548582A (en) * | 2013-11-01 | 2014-02-05 | 中国热带农业科学院橡胶研究所 | A kind of seedling cultivation method of bare root bud grafting of rubber seedlings |
| CN103743915A (en) * | 2014-01-20 | 2014-04-23 | 云南省热带作物科学研究所 | Identification method for high-quality seedling of rubber tree |
| CN103947448A (en) * | 2014-05-20 | 2014-07-30 | 中国热带农业科学院橡胶研究所 | Method for increasing budding and growing consistency of bud grafting seedlings of rubber seeds |
| CN104429628A (en) * | 2014-10-27 | 2015-03-25 | 中国热带农业科学院橡胶研究所 | Method for shortening flowering stage of rubber tree hybridization parents |
| CN105613062A (en) * | 2016-03-22 | 2016-06-01 | 中国热带农业科学院橡胶研究所 | Rubber superior tree tender shoot grafting, rejuvenizing and seedling growing method |
| CN107347557A (en) * | 2017-08-01 | 2017-11-17 | 西双版纳丽欣农业科技有限责任公司 | Rubber tree budling nursery is bred and rejuvenation thereafter and method for changing |
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| CN105746282B (en) * | 2016-03-22 | 2018-07-27 | 中国热带农业科学院橡胶研究所 | Rubber tree budling nursery proliferation and rejuvenation thereafter and method for changing |
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| CN118318622A (en) * | 2024-05-17 | 2024-07-12 | 中国热带农业科学院橡胶研究所 | A method for cultivating rubber tree rootstock seedlings using seed axillary buds |
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| CN1312974C (en) * | 2005-03-30 | 2007-05-02 | 中国热带农业科学院热带生物技术研究所 | A clone micropropagation method for rubber tree |
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2006
- 2006-08-11 CN CN2006101152473A patent/CN101120641B/en not_active Expired - Fee Related
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388801B (en) * | 2011-07-27 | 2013-07-24 | 青岛农业大学 | Sterile grafting method of peanut tissue culture seedlings |
| CN102388801A (en) * | 2011-07-27 | 2012-03-28 | 青岛农业大学 | Sterile grafting method of peanut tissue culture seedlings |
| CN102388735A (en) * | 2011-07-28 | 2012-03-28 | 广东农垦热带作物科学研究所 | Small-sized green budling batch multiplication method for rubber trees |
| CN102388735B (en) * | 2011-07-28 | 2013-01-02 | 广东农垦热带作物科学研究所 | Small-sized green budling batch multiplication method for rubber trees |
| CN103125382A (en) * | 2011-12-02 | 2013-06-05 | 云南省热带作物科学研究所 | Micropropagation method for rubber tree good variety somatic embryo plant |
| CN102696390A (en) * | 2012-07-03 | 2012-10-03 | 广东农垦热带作物科学研究所 | Method for promoting budding of seedling budlings of Brazil rubber trees |
| CN102696390B (en) * | 2012-07-03 | 2013-10-23 | 广东农垦热带作物科学研究所 | Method for promoting budding of seedling budlings of Brazil rubber trees |
| CN102792856A (en) * | 2012-07-11 | 2012-11-28 | 云南省红河热带农业科学研究所 | Jackfruit juvenile bud grafting method |
| CN102860221B (en) * | 2012-07-14 | 2014-06-18 | 中国热带农业科学院橡胶研究所 | Rubber tree grafting seedling method |
| CN102860221A (en) * | 2012-07-14 | 2013-01-09 | 中国热带农业科学院橡胶研究所 | Rubber tree seedling cultivation method |
| CN103548582A (en) * | 2013-11-01 | 2014-02-05 | 中国热带农业科学院橡胶研究所 | A kind of seedling cultivation method of bare root bud grafting of rubber seedlings |
| CN103743915A (en) * | 2014-01-20 | 2014-04-23 | 云南省热带作物科学研究所 | Identification method for high-quality seedling of rubber tree |
| CN103947448A (en) * | 2014-05-20 | 2014-07-30 | 中国热带农业科学院橡胶研究所 | Method for increasing budding and growing consistency of bud grafting seedlings of rubber seeds |
| CN104429628A (en) * | 2014-10-27 | 2015-03-25 | 中国热带农业科学院橡胶研究所 | Method for shortening flowering stage of rubber tree hybridization parents |
| CN104429628B (en) * | 2014-10-27 | 2017-06-16 | 中国热带农业科学院橡胶研究所 | The method for shortening rubber tree hybrid strain florescence |
| CN105613062A (en) * | 2016-03-22 | 2016-06-01 | 中国热带农业科学院橡胶研究所 | Rubber superior tree tender shoot grafting, rejuvenizing and seedling growing method |
| CN105746282B (en) * | 2016-03-22 | 2018-07-27 | 中国热带农业科学院橡胶研究所 | Rubber tree budling nursery proliferation and rejuvenation thereafter and method for changing |
| CN107347557A (en) * | 2017-08-01 | 2017-11-17 | 西双版纳丽欣农业科技有限责任公司 | Rubber tree budling nursery is bred and rejuvenation thereafter and method for changing |
| CN107567848A (en) * | 2017-10-19 | 2018-01-12 | 四川省林业科学研究院 | A kind of walnut summer budding method |
| CN113383654A (en) * | 2021-07-01 | 2021-09-14 | 云南省热带作物科学研究所 | Passion flower micro bud seedling grafting method |
| CN118318622A (en) * | 2024-05-17 | 2024-07-12 | 中国热带农业科学院橡胶研究所 | A method for cultivating rubber tree rootstock seedlings using seed axillary buds |
| CN118318622B (en) * | 2024-05-17 | 2024-10-29 | 中国热带农业科学院橡胶研究所 | Method for cultivating rubber tree stock seedlings by adopting seed axillary buds |
| CN119836946A (en) * | 2025-03-07 | 2025-04-18 | 云南省热带作物科学研究所 | Method for improving survival rate of bud grafting of big rubber tree |
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