CN101091444A - Apparatus capable of controlling density of CO2 for culturing growth of plant - Google Patents

Apparatus capable of controlling density of CO2 for culturing growth of plant Download PDF

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Publication number
CN101091444A
CN101091444A CNA2007100701001A CN200710070100A CN101091444A CN 101091444 A CN101091444 A CN 101091444A CN A2007100701001 A CNA2007100701001 A CN A2007100701001A CN 200710070100 A CN200710070100 A CN 200710070100A CN 101091444 A CN101091444 A CN 101091444A
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concentration
plant growing
pipe
gas
control
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CNA2007100701001A
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CN100558227C (en
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都韶婷
章永松
樊琳
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

The present invention discloses a plant growth culture equipment capable of controlling CO2 concentration. It includes a sealed plant growth chamber component with opening device. It is made of transparent material, one end of its outet tube is communicated with the internal cavity of said plant growth chamber component, and its another end is connected with CO2 gas balance component by successively means of three-way switching valve I, tube I, air pump and air outlet tube, the CO2 gas balance component is connected with internal cavity of said plant growth chamber component by successively means of tube II, air buffer tank, tube IV, CO2 detection component, tube III, three-way switching valve II and inlet tube; two ends of connecting tube are respectively connected with three-way switching valve I and three-way switching valve II. The application of said invention can raise accurate control of CO2 concentration.

Description

A kind ofly can control CO 2The plant growing culture apparatus of concentration
Technical field
The present invention relates to a kind ofly can control CO 2The plant growing culture apparatus of concentration.
Background technology
Now, Atmospheric CO 2Concentration raise and to cause Global climate change to become the focus that people pay close attention to; Therefore, relevant CO 2The concentration rising also becomes present research focus to the influence of plant growing, to the CO of growing process 2It is the key of carrying out this respect research that concentration is accurately controlled.Yet, the existing in the market CO that is applicable to plant growing 2The incubator kind seldom, not only cost an arm and a leg (quality every price preferably reaches hundreds thousand of units), and CO 2The concentration control accuracy not high (± 75ppm), be difficult to satisfy some to test processing accuracy high requirement.And, CO in system 2Concentration requirement is lower than Atmospheric CO 2During concentration, existing C O 2Incubator can't be realized.In addition, existing C O 2Incubator also often occurs causing phenomenons such as plant undergrowth because of the intensity of illumination deficiency.
Summary of the invention
The technical problem to be solved in the present invention provides a kind ofly can control CO 2The plant growing culture apparatus of concentration uses this device to improve CO 2The accurate control of concentration can also solve the problem of natural lighting deficiency.
In order to solve the problems of the technologies described above, to the invention provides and a kind ofly can control CO 2The plant growing culture apparatus of concentration comprises plant growing chamber component sealing and that be provided with opening device, also comprises threeway changeover valve I, the air pump that has escape pipe, CO 2Gas balance assembly, gas buffer jar, CO 2Detection components and threeway changeover valve II, the plant growing chamber component is made by transparent material; An end that goes out pipe communicates with the inner chamber of plant growing chamber component, the other end successively by threeway changeover valve I, the pipe I, air pump and escape pipe and CO 2The gas balance assembly links to each other, CO 2The gas balance assembly is successively by pipe II, gas buffer jar, pipe IV, CO 2Detection components, pipe III, threeway changeover valve II link to each other with the inner chamber of inlet pipe with the plant growing chamber component; The two ends of tube connector link to each other with threeway changeover valve II with threeway changeover valve I respectively.
Can control CO as of the present invention 2The improvement of the plant growing culture apparatus of concentration: CO 2The gas balance assembly comprises gas balance groove, pH meter, accent alkali bottle and the acid adjustment bottle of sealing, is provided with pneumatic head and pH electrode in the gas balance groove; Transfer alkali bottle and acid adjustment bottle respectively with the intracavity inter-connection of gas balance groove, pH meter links to each other with the pH electrode; Escape pipe links to each other with pneumatic head, the intracavity inter-connection of pipe II and gas balance groove.
Can control CO as of the present invention 2The further improvement of the plant growing culture apparatus of concentration: CO 2Detection components comprises the CO that links to each other successively 2Detector, CO 2Inductor and CO 2Concentration sensing chamber, CO 2Concentration sensing chamber is connected with pipe III and pipe IV respectively.
Can control CO as of the present invention 2The further improvement of the plant growing culture apparatus of concentration: the plant growing chamber component comprises that growth room's base and bottom are provided with the phytotron of opening, is provided with the water sealed tank of " returning " type on growth room's base, and the opening and the water sealed tank of phytotron match.
Can control CO as of the present invention 2The further improvement of the plant growing culture apparatus of concentration: the indoor thermometer that is provided with of plant growing.
Can control CO as of the present invention 2The further improvement of the plant growing culture apparatus of concentration: be provided with gas blender in the gas buffer jar.
Can control CO as of the present invention 2The further improvement of the plant growing culture apparatus of concentration: the gas buffer jar compares 〉=10 with the cavity volume of phytotron.
Of the present inventionly can control CO 2The plant growing culture apparatus of concentration is according under Stationary pH and temperature condition, CO in sodium bicarbonate solution and the air 2There is the principle of balanced relation in dividing potential drop; And adopt the method for buffer unit, through development test gained repeatedly repeatedly.It is required CO in the controlling plant process of growth effectively 2Concentration makes CO 2The control accuracy of concentration obviously improves (in ± 50ppm).And because the plant growing chamber component is made by transparent material, so the growth of plant can directly adopt lamp, promptly solved the not enough problem that influences plant growing of intensity of illumination.Advantage of the present invention is specific as follows:
1), as long as the pH by sodium bicarbonate solution in the adjustments of gas compensating groove can reach CO in the control system 2The purpose of concentration is a kind of brand-new CO 2Concentration control method.
CO among the present invention 2The design of gas balance assembly is a kind of brand-new CO 2Concentration control method.In the prior art, regulate CO in the control growing case 2The method of concentration is mainly by directly applying CO 2Gas realizes that this method is CO not only 2Concentration wayward (fluctuation is big), and can't realize as test requirements document CO 2Concentration is lower than Atmospheric CO 2The requirement of concentration.And control method of the present invention has adopted CO 2The pH control method of gas balance liquid, not only simple, the CO of control method 2Fluctuation of concentration is little, and can satisfy CO in the realization system 2Concentration is lower than Atmospheric CO 2The requirement that the concentration test is handled.
2), the present invention is provided with and is used to store CO 2The gas buffer jar because the volume of the volume ratio phytotron of gas buffer jar is much bigger, to the CO of whole system 2Change in concentration can play cushioning effect preferably, thereby can improve greatly whole system CO 2The control accuracy of concentration.
Because CO of the present invention 2It is little that gas balance is regulated fluctuation of concentration, adds the setting of gas buffer jar, thereby make CO 2The control accuracy of concentration significantly improves, at present commercialization CO 2The control accuracy of growth case is ± 75ppm, and control accuracy of the present invention can reach ± 50ppm in.
3), phytotron adopts transparent material to make, therefore can adopt lamp to carry out the plant cultivation, both solved plant undergrowth often appears in climatic cabinate because of the intensity of illumination deficiency defective, can make condition of culture more near the field actual conditions again.The water sealed tank of designed " returning " type had both guaranteed the sealing of whole system, can very conveniently open again the plant growing chamber component to plant water, take a sample, operation such as mensuration.
Phytotron of the present invention can arbitrarily design its size according to test requirements document, can satisfy the cultivation requirement of the very big different plants of individual difference.And existing commercialization CO 2The inner bag space of growth case is fixed, and can't satisfy above-mentioned requirements.
4), cost of manufacture of the present invention is cheap, package unit only needs 5000 yuans; And existing quality commercialization CO preferably 2Every price of growth case is up to hundreds thousand of yuans.
Description of drawings
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in further detail.
Fig. 1 of the present inventionly can control CO 2The structural representation of the plant growing culture apparatus of concentration.
Embodiment
Fig. 1 has provided and a kind ofly can control CO 2The plant growing culture apparatus of concentration comprises plant growing chamber component 2 sealing and that be provided with opening device, threeway changeover valve I 5, has air pump 7, the CO of escape pipe 71 2Gas balance assembly 8, gas buffer jar 10, CO 2Detection components 12 and threeway changeover valve II13.
Plant growing chamber component 2 sealing and that be provided with opening device comprises growth room's base 21 and phytotron 22.Phytotron 22 is that the cuboid box of (promptly not having the bottom) is opened wide in a bottom surface, so unlimited place, bottom surface promptly forms opening 221.Phytotron 22 can be made of the 3mm transparent plexiglass plate, and size can be determined according to the size of test plant.One end of thermometer 3 passes the end face of phytotron 22, is positioned at the inner chamber of phytotron 22; Be used to detect the temperature of phytotron 22 inner chambers.In order to guarantee the sealing of whole device, should keep seamlessly contacting between the end face of thermometer 3 and phytotron 22.Growth room's base 21 can be made of the thick PVC plate of 5mm, and it is long and wide respectively than length and wide each big 15mm of phytotron 22 cross sections.Two of welding are big square frame and the little square frame that the thick PVC plate of 5mm is made and highly equated on growth room's base 21, and little square frame is enclosed within the big square frame, and the center overlaid of little square frame and big square frame; Therefore formed the water sealed tank 23 of " a returning " type, the degree of depth of water sealed tank 23 is 8cm (height that is big square frame and little square frame is 8cm).
The opening 221 of phytotron 22 can insert in this water sealed tank 23 freely; At this moment, leave the space of 1-2mm between the inner surface of the outer surface of phytotron 22 and big square frame, also leave the gap of 1-2mm between the inner surface of phytotron 22 and the outer surface of little square frame.
Set out gas port at the right flank of phytotron 22 near the height place of end face 5cm, an end that goes out pipe 4 gas outlet sealing therewith links to each other; Therefore, go out the intracavity inter-connection of pipe 4 by this gas outlet and phytotron 22.Set into gas port at the left surface of phytotron 22 near the height place of bottom 10cm, this air intake is positioned at the diagonally opposing corner direction of gas outlet, and an end of inlet pipe 1 air intake sealing therewith links to each other; Therefore, inlet pipe 1 is by the intracavity inter-connection of this air intake and phytotron 22.
CO 2Gas balance assembly 8 comprises gas balance groove 82, pH meter 84, accent alkali bottle 85 and the acid adjustment bottle 86 of sealing, the pH meter 84 optional common pH meters of putting two place readings decimally.Gas balance groove 82 can adopt the resealable container (as vial, barrelled water bucket etc.) about 10 liters to add as the rubber stopper of sealing-plug to form.In gas balance groove 82, be provided with pneumatic head 81 and pH electrode 83; The line of pH electrode 83 links to each other with the pH meter 84 that is positioned at gas balance groove 82 outsides after passing rubber stopper.Transfer an end of the woven hose of the end of woven hose of alkali bottle 85 and acid adjustment bottle 86 to pass the inner chamber that is positioned at gas balance groove 82 behind the rubber stopper respectively.Be gapless the contact between the woven hose of the line of pH electrode 83, the woven hose of transferring alkali bottle 85 and acid adjustment bottle 86 and the rubber stopper.
CO 2Detection components 12 comprises continuous CO 2Detector 121 and CO 2Inductor 122; CO 2One end of inductor 122 is positioned at CO 2In the concentration sensing chamber 123.CO 2 Detector 121 can be selected conventional portable CO for use 2Detector.
The other end that goes out pipe 4 successively by threeway changeover valve I5, manage I6, air pump 7 links to each other with escape pipe 71, the other end of escape pipe 71 links to each other with pneumatic head 81 after passing rubber stopper on the gas balance groove 82.The end of pipe II9 passes the inner chamber that is positioned at gas balance groove 82 behind the rubber stopper on the gas balance groove 82, and the other end is connected with the top of the inner chamber of gas buffer jar 10.Equally, be gapless the contact between escape pipe 71 and pipe II9 and the rubber stopper.
Top at gas buffer jar 10 inner chambers is fixed with gas blender 15, and this gas blender 15 is specially a little ceiling fan, and the subsidiary electric wire of this little ceiling fan passes the outside that is positioned at gas buffer jar 10 behind the top of gas buffer jar 10; In order to keep sealing, this electric wire must contact with the top of gas buffer jar 10 is gapless.Little ceiling fan plays gas blender, with the gas in the full and uniform mist surge tank 10.Gas buffer jar 10 can be selected civilian roof stainless steel water jar for use.Under the conditions permit situation, the volume of gas buffer jar 10 is big more, control CO 2The precision of concentration is just high more; Therefore gas buffer jar 10 and the cavity volume of phytotron 22 are than best 〉=10.
The end of pipe IV16 is connected with the bottom of the inner chamber of gas buffer jar 10, and the other end then passes through CO successively 2 Concentration sensing chamber 123, pipe III11 are connected with threeway changeover valve II13.Threeway changeover valve II13 is connected with inlet pipe 1, and threeway changeover valve II 13 also is connected with threeway changeover valve I 5 by tube connector 14.
Of the present inventionly can control CO 2The plant growing culture apparatus of concentration, the concrete course of work is as follows:
1, in transferring alkali bottle 85, pack into the NaOH solution of 1mol, the HCl solution of the 1mol that in acid adjustment bottle 86, packs into.
2, the 5 liters of 0.5mol NaHCO that in gas balance groove 82, pack into 3Solution makes the electrode tip of pH electrode 83 just immerse NaHCO 3Solution.Make pneumatic head 81 can sink to NaHCO in the gas balance groove 82 3The bottom of solution.
3, test plant is put into little square frame on growth room's base 21, the opening 221 with phytotron 22 inserts in the water sealed tanks 23 then; Fill water sealed tank 23 with 0.01mol HCl solution.Because 0.01mol HCl solution can prevent that water-soluble separating from absorbing CO 2, therefore can make whole device form an airtight gas system capable of circulation.
4, CO 2The debugging of concentration:
Earlier rotate threeway changeover valve I5 and threeway changeover valve II13 respectively, pipe I6, tube connector 14 and pipe III11 are communicated with; At this moment, threeway changeover valve I5 does not communicate with going out pipe 4, and threeway changeover valve II13 does not communicate with inlet pipe 1.
Open pH meter 84 (this pH meter 84 needs to demarcate with the pH titer in advance), little ceiling fan (being gas blender 15) and CO more respectively 2The power supply of detector 121; And open air pump 7, the gas flow of control air pump 7 is about 20L/ minute.
At this moment, gas will be successively enters NaHCO in the gas balance groove 82 by escape pipe 71 and pneumatic head 81 3Solution carries out CO 2Balance; PH meter 84 can be by the NaHCO in 83 pairs of gas balance grooves of pH electrode 82 3Solution detects.Gas in the gas balance groove 82 enters gas buffer jar 10 through pipe II 9 again, and under the mixed uniformly effect of little ceiling fan, gas is then by pipe IV16, CO 2 Concentration sensing chamber 123 and pipe III11 enter threeway changeover valve II13, successively by tube connector 14, threeway changeover valve I5, pipe I6, return at last into air pump 7 again, form an airtight gas return path.IV16 enters CO from pipe 2The gas of concentration sensing chamber 123 is by CO 2Inductor 122 inductions can be by CO 2Detector 121 detects its CO 2Concentration.
Work as CO 2When detector 121 and pH meter 84 stable reading, illustrate that the concentration in the whole system has reached balance.Handle required CO according to plant growing then 2Set concentration and carry out the CO of system 2The adjusting of concentration.CO as if system's this moment 2Concentration is lower than setting concentration, can open the drip switch of acid adjustment bottle 86, makes HCl solution in the acid adjustment bottle 86 slowly splash into NaHCO in the gas balance groove 82 3In the solution; CO in the system like this 2Concentration begins to rise gradually, as the CO that reaches setting soon 2Slow down during concentration or intermittently add acid, until the CO of system 2Concentration arrives to be set till the concentration.CO as if system's this moment 2Concentration is higher than when setting concentration, then enables and transfers alkali bottle 85, method to be analogous to use acid adjustment bottle 86; Purpose is to make CO 2Concentration descends gradually.Whole debug process preferably can recording system at different CO 2CO when concentration conditions is issued to balance 2Concentration, pH value and temperature data can utilize these data to make NaHCO under this temperature 3PH value of solution and the CO of system 2The relation curve of concentration can be found system from this curve and reach a certain CO 2Pairing NaHCO during concentration value 3The pH value of solution value can be used as CO 2The reference of concentration set point debugging.
Attention: need there be enough height acid adjustment bottle 86 and the position of transferring alkali bottle 85 to hang, otherwise can cause in the bottle acid or alkali to ooze because of there is a small pressure difference in system, even acid, alkali lye take place from acid adjustment bottle 86 with transfer the danger of the corresponding air inlet tubule ejection of alkali bottle 85.
5, plant growing is cultivated and CO 2Concentration control:
Rotate threeway changeover valve I5, use pipe 4 and be communicated with pipe I6; At this moment, threeway changeover valve I5 does not communicate with tube connector 14.Rotate threeway changeover valve II13, pipe III11 is communicated with inlet pipe 1; At this moment, threeway changeover valve II13 does not communicate with tube connector 14.Good gas circulates by phytotron 22 to make debugging, realizes the CO to phytotron 22 2Concentration is controlled.Gas flow is specific as follows:
Gas in the gas buffer jar 10 is successively by pipe IV16, CO 2 Concentration sensing chamber 123, pipe III11, threeway changeover valve II13 and inlet pipe 1 enter in the phytotron 22; And then enter NaHCO in the gas balance groove 82 by going out pipe 4, threeway changeover valve I5, pipe I6, air pump 7, escape pipe 71 and pneumatic head 81 successively 3Solution carries out CO 2Balance, the gas in the gas balance groove 82 enter gas buffer jar 10 by pipe II9 again, thereby form an airtight gas return path.
6, in incubation, need the CO of system every morning 2Concentration is carried out an adjustment, promptly carries out the work of above-mentioned steps 4.The volume of gas buffer jar 10 hour preferably can also carry out the adjustment work of a step 4 at noon if the plant growing amount is big.
NaHCO in the gas balance groove 82 3Solution needs to change once weekly.
If will transfer the drip switch of alkali bottle 85 and acid adjustment bottle 86 all to be set at automatic titration device, again with above-mentioned 2 automatic titration devices by holding wire respectively with CO 2Detector 121 or pH meter 84 link to each other, and then can realize the purpose of control fully automatically of the present invention.
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.

Claims (7)

1, a kind ofly can control CO 2The plant growing culture apparatus of concentration comprises plant growing chamber component sealing and that be provided with opening device (2), it is characterized in that: also comprise threeway changeover valve I (5), have air pump (7), the CO of escape pipe (71) 2Gas balance assembly (8), gas buffer jar (10), CO 2Detection components (12) and threeway changeover valve II (13), described plant growing chamber component (2) is made by transparent material; An end that goes out pipe (4) communicates with the inner chamber of plant growing chamber component (2), the other end successively by threeway changeover valve I (5), manage I (6), air pump (7) and escape pipe (71) and CO 2Gas balance assembly (8) links to each other, described CO 2Gas balance assembly (8) is successively by pipe II (9), gas buffer jar (10), pipe IV (16), CO 2Detection components (12), pipe III (11), threeway changeover valve II (13) link to each other with the inner chamber of inlet pipe (1) with plant growing chamber component (2); The two ends of tube connector (14) link to each other with threeway changeover valve II (13) with threeway changeover valve I (5) respectively.
2, according to claim 1ly can control CO 2The plant growing culture apparatus of concentration is characterized in that: described CO 2Gas balance assembly (8) comprises gas balance groove (82), pH meter (84), accent alkali bottle (85) and the acid adjustment bottle (86) of sealing, is provided with pneumatic head (81) and pH electrode (83) in the described gas balance groove (82); Transfer alkali bottle (85) and acid adjustment bottle (86) respectively with the intracavity inter-connection of gas balance groove (82), pH meter (84) links to each other with pH electrode (83); Escape pipe (71) links to each other with pneumatic head (81), the intracavity inter-connection of pipe II (9) and gas balance groove (82).
3, according to claim 2ly can control CO 2The plant growing culture apparatus of concentration is characterized in that: described CO 2Detection components (12) comprises the CO that links to each other successively 2Detector (121), CO 2Inductor (122) and CO 2Concentration sensing chamber (123), described CO 2Concentration sensing chamber (123) is connected with pipe III (11) and pipe IV (16) respectively.
4, according to claim 3ly can control CO 2The plant growing culture apparatus of concentration, it is characterized in that: described plant growing chamber component (2) comprises that growth room's base (21) and bottom are provided with the phytotron (22) of opening (221), be provided with the water sealed tank (23) of " returning " type on growth room's base (21), the opening (221) of phytotron (22) matches with water sealed tank (22).
5, according to claim 4ly can control CO 2The plant growing culture apparatus of concentration is characterized in that: be provided with thermometer (3) in the described phytotron (22).
6, according to claim 5ly can control CO 2The plant growing culture apparatus of concentration is characterized in that: be provided with gas blender (15) in the described gas buffer jar (10).
7, according to claim 6ly can control CO 2The plant growing culture apparatus of concentration is characterized in that: the cavity volume of described gas buffer jar (10) and phytotron (22) is than 〉=10.
CNB2007100701001A 2007-07-20 2007-07-20 A kind ofly can control CO 2The plant growing culture apparatus of concentration Expired - Fee Related CN100558227C (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102144502A (en) * 2010-12-17 2011-08-10 青岛农业大学 Device for continuously supplying CO2 for open top type plant growth assimilation box
CN102349436A (en) * 2011-08-26 2012-02-15 中国航天员科研训练中心 Plant cultivation system under environmental condition of controlled ecological life support system
CN102960200A (en) * 2012-10-26 2013-03-13 西安金诺光电科技有限公司 Automatic adjusting device and method for concentration of CO2 in greenhouse environment
CN104255342A (en) * 2014-06-13 2015-01-07 张家港永联天天鲜配送有限公司 Garden house ventilation and air change equipment
CN108031238A (en) * 2018-01-18 2018-05-15 上海孙桥农业科技股份有限公司 Capture device and application method that carbon dioxide in air is used for industrialized agriculture
CN111802129A (en) * 2020-07-20 2020-10-23 湖州灵粮生态农业有限公司 Plant seedling raising device and seedling raising method
CN114561292A (en) * 2022-04-27 2022-05-31 天津长和生物技术有限公司 Cell culture case with from drum-type carbon dioxide preparation facilities

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102144502A (en) * 2010-12-17 2011-08-10 青岛农业大学 Device for continuously supplying CO2 for open top type plant growth assimilation box
CN102144502B (en) * 2010-12-17 2012-12-12 青岛农业大学 Device for continuously supplying CO2 for open top type plant growth assimilation box
CN102349436A (en) * 2011-08-26 2012-02-15 中国航天员科研训练中心 Plant cultivation system under environmental condition of controlled ecological life support system
CN102349436B (en) * 2011-08-26 2013-11-06 中国航天员科研训练中心 Plant cultivation system under environmental condition of controlled ecological life support system
CN102960200A (en) * 2012-10-26 2013-03-13 西安金诺光电科技有限公司 Automatic adjusting device and method for concentration of CO2 in greenhouse environment
CN104255342A (en) * 2014-06-13 2015-01-07 张家港永联天天鲜配送有限公司 Garden house ventilation and air change equipment
CN108031238A (en) * 2018-01-18 2018-05-15 上海孙桥农业科技股份有限公司 Capture device and application method that carbon dioxide in air is used for industrialized agriculture
CN108031238B (en) * 2018-01-18 2024-03-29 上海孙桥农业科技股份有限公司 Device for capturing carbon dioxide in atmosphere for facility agriculture and using method
CN111802129A (en) * 2020-07-20 2020-10-23 湖州灵粮生态农业有限公司 Plant seedling raising device and seedling raising method
CN114561292A (en) * 2022-04-27 2022-05-31 天津长和生物技术有限公司 Cell culture case with from drum-type carbon dioxide preparation facilities

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