CN114561292A - Cell culture case with from drum-type carbon dioxide preparation facilities - Google Patents

Cell culture case with from drum-type carbon dioxide preparation facilities Download PDF

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Publication number
CN114561292A
CN114561292A CN202210450800.8A CN202210450800A CN114561292A CN 114561292 A CN114561292 A CN 114561292A CN 202210450800 A CN202210450800 A CN 202210450800A CN 114561292 A CN114561292 A CN 114561292A
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Prior art keywords
sleeve
matching
rod
circulating
sliding
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Granted
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CN202210450800.8A
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CN114561292B (en
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王秀娟
牟春琳
倪琳
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Tianjin Changhe Biological Technology Co ltd
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Tianjin Changhe Biological Technology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00Combinations of bioreactors or fermenters with other apparatus
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

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Abstract

The invention belongs to the technical field of chemistry and metallurgy, in particular to a cell culture box with a self-drum carbon dioxide preparation device, which solves the technical problem that the calcium oxide generated after the existing industrial calcium carbonate is calcined at high temperature can influence the heating of the calcium carbonate and cause the low generation efficiency without any method, and also solves the problem that a carbon dioxide gas cylinder needs to be additionally arranged on the carbon dioxide culture box; meanwhile, the particles in the gas are removed through the filter medium in the communication assembly, and the particles are rewashed and discharged back to continue the reaction.

Description

Cell culture case with from drum-type carbon dioxide preparation facilities
Technical Field
The invention belongs to the technical field of chemistry and metallurgy, and particularly relates to a cell culture box with a self-drum carbon dioxide preparation device.
Background
Carbon dioxide has high civil and industrial value, is widely applied in a plurality of fields, and is a valuable resource. The method is not only widely applied to the aspects of petroleum exploitation, metallurgy, welding, low-temperature refrigerant, mechanical manufacturing, artificial rainfall, fire protection, chemical engineering, papermaking, agriculture, food industry, medical health and the like, but also can be applied to the advanced scientific and technological fields of supercritical solvents, bioengineering, laser counting, nuclear industry and the like. Of all these applications, food grade carbon dioxide is most widely used, and carbon dioxide used in advanced technology is also obtained by repurifying food grade carbon dioxide; therefore, large-scale production of food grade carbon dioxide is essential;
for example, the patent No. CN201721666100.3 discloses a preparation apparatus for food-grade carbon dioxide, which comprises a carbon dioxide preparation apparatus, a carbon dioxide detection apparatus, and a carbon dioxide purification apparatus, wherein the carbon dioxide preparation apparatus comprises a reaction cylinder, a sealed plastic, a reaction cylinder cover, a material reaction plate, a stirring apparatus, a liquid level sensor, an air outlet, and a gas switch valve, the purification apparatus comprises a washing tower, a compressor, an adsorption tower, a drying tower, a condenser, a distiller, and a collection tank, the carbon dioxide detection apparatus comprises an online carbon dioxide detection apparatus and a carbon dioxide sampling port, the sealed plastic is located on the reaction cylinder cover, the stirring apparatus is installed on and under the material reaction plate, the liquid level sensor is placed inside the reaction cylinder, the air outlet is connected to the carbon dioxide detection apparatus and the carbon dioxide purification apparatus respectively, but the technical solution has the disadvantage that no solution is available for solving the problem after the industrial calcium carbonate is calcined at high temperature, the generated calcium oxide can affect the heating of calcium carbonate, and the generation efficiency is low.
Disclosure of Invention
The invention aims to provide a cell culture box with a self-drum carbon dioxide preparation device, which solves the technical problem that calcium oxide generated after the existing industrial calcium carbonate is calcined at high temperature can influence the heating of the calcium carbonate to cause low generation efficiency without any method, and also solves the problem that a carbon dioxide culture box needs to be additionally provided with a carbon dioxide gas cylinder.
In order to achieve the purpose, the specific technical scheme of the cell culture box with the self-drum carbon dioxide preparation device is as follows:
a cell culture box with a self-drum carbon dioxide preparation device comprises a high-temperature sleeve component, a base, a cell culture box component, a sealing sleeve, a three-way sleeve, external connecting pipes and a high-temperature sleeve component controller, wherein the high-temperature sleeve component is arranged at the inner end of the sealing sleeve, the sealing sleeve is arranged on the base, the sealing sleeve is provided with the three-way sleeve and is communicated with the three-way sleeve, the three-way sleeve is provided with two external connecting pipes which are communicated with each other, the cell culture box component is provided with two cell culture box components which are stacked on the base, the cell culture box component is connected and communicated with the corresponding external connecting pipes, the cell culture box further comprises a communicating component, a circulating component, a mounting sleeve A, a mounting sleeve connecting rod and an adjusting component, the high-temperature sleeve component is connected with the communicating component, the adjusting component is connected with the high-temperature sleeve component, and the communicating component is connected with the circulating component, the circulation assembly is installed on the adjusting assembly through an installation sleeve A and an installation sleeve connecting rod, the installation sleeve A is fixedly connected with the installation sleeve connecting rod, and the communicating assembly is communicated with the three-way sleeve.
Further, cell culture case subassembly includes box, hand power box, cultivates casket and outer end stop screw, it has air vent one and air vent two to open on the box, air vent one and one of them external connecting tube intercommunication, air vent two and other external connecting tube intercommunication among them, hand power box slidable mounting is in the box inner, it has a plurality of rectangle spouts to open on the hand power box, cultivates and rotates a plurality of rollers of installation on the casket, cultivates the casket and passes through a plurality of roller slidable mounting in the rectangle spout, it has the mounting hole to open on the hand power box, outer end stop screw thread fit installs in the mounting hole and spacing locking hand power box, rectangle spout and the mounting hole intercommunication that corresponds.
Furthermore, a display screen and a handle which is convenient for pulling the hand pulling box body are arranged on the hand pulling box body.
Furthermore, the culture box is provided with a groove with a convex edge, and the culture box is provided with a rectangular vent hole for ventilation and a placing groove for placing a cell culture dish.
Further, the high temperature sleeve subassembly includes outer sleeve, lower extreme cover, inner rotating sleeve, link, puddler, turbofan blade, blade axle, awl tooth one, awl tooth two and spacing cyclic annular boss, the lower extreme of outer sleeve is installed, and the inner of outer sleeve is provided with spacing cyclic annular boss, rotates on the spacing cyclic annular boss and installs inner rotating sleeve, and the link is installed to inner rotating sleeve the inner, installs a plurality of puddlers on the link, installs awl tooth two on the inner rotating sleeve, and awl tooth two and awl tooth one mesh transmission, the one end at the blade axle is installed to awl tooth one, and the turbofan blade is installed to the other end of blade axle, and the blade axle rotates the installation with the outer sleeve, the outer sleeve is connected with the intercommunication subassembly, and the adjusting part is connected with the outer sleeve.
Furthermore, a feeding opening is formed in the outer sleeve, and a plug is connected to the inner thread of the feeding opening.
Furthermore, a plurality of stirring rods are circumferentially arranged on the connecting frame along the central axis of the outer sleeve in an array mode.
Further, a high-temperature heating device is arranged on the lower end cover.
Further, the communicating component comprises a communicating sleeve, a first connecting pipe, a first filtering sleeve, a second filtering sleeve, a first sealing end cover, a second connecting pipe, a third connecting pipe, a second sealing end cover, a fourth connecting pipe, an external connecting pipe and a connecting sleeve, one end of the communicating sleeve is arranged on the outer sleeve, the outer sleeve is provided with a plurality of fan-shaped through holes, the communicating sleeve is communicated with the fan-shaped through holes, the other end of the communicating sleeve is arranged on the first connecting pipe, the first filtering sleeve is arranged at one end of the second filtering sleeve, the other end of the first filtering sleeve is provided with the first sealing end cover, one end of the second connecting pipe is arranged on the first sealing end cover, the other end of the second connecting pipe is arranged on the outer sleeve, the first connecting pipe is communicated with the first through hole, the first sealing end cover is connected with the circulating component, one end of the third connecting pipe is arranged on the outer sleeve, the second through hole is arranged on the outer sleeve, and the second through hole is communicated with the third connecting pipe, the other end of the connecting pipe III is installed on a sealing end cover II, the sealing end cover II is connected with the circulating assembly, one end of the connecting pipe IV is installed on the sealing end cover II, the other end of the connecting pipe IV is installed on the connecting sleeve, and the connecting sleeve is provided with an external connecting pipe through thread matching.
Furthermore, a sealing ring is arranged in the connecting sleeve, and the sealing ring is connected with the connecting sleeve through a spring I.
Further, the circulating component comprises a circulating sleeve I, a first matching bevel gear, a connecting mounting sleeve, a mounting block sliding rod, a second circulating sleeve, a second matching bevel gear, a first circulating cavity, a first circulating push plate, a first push plate sliding rod, a first sliding rod matching hemisphere, a first push plate sliding rod push spring, a first matching disc sliding groove, a first matching block sliding rod, a second circulating cavity, a second circulating push plate, a second push plate sliding rod, a second sliding rod matching hemisphere, a second push plate sliding rod push spring, a second matching disc sliding groove, a second matching block and a second matching block sliding rod, the first sealing end cover is rotatably mounted on the first circulating sleeve, a first sleeve convex edge is arranged on the first circulating sleeve, the first circulating sleeve is rotatably limited on the connecting mounting sleeve through the first sleeve convex edge, the first matching bevel gear is mounted on the first circulating sleeve, and the first circulating sleeve is provided with a plurality of circulating cavities, a first circulating push plate is slidably mounted at the inner end of the first circulating cavity, the first circulating push plate is mounted at one end of a first push plate slide bar, a first slide bar matching hemisphere is mounted at the other end of the first push plate slide bar, the first push plate slide bar is in sliding fit with the first circulating sleeve, a first push plate slide bar push spring is sleeved on the first push plate slide bar, the first push plate slide bar push spring is arranged between the first slide bar matching hemisphere and the first circulating sleeve, the first slide bar matching hemisphere is attached to a first matching plate, the first matching plate is rotatably mounted with a connecting mounting sleeve, a first matching plate slide groove is formed in the first matching plate, a first matching block is connected to the inner end of the first matching plate slide groove in sliding fit, the first matching block is slidably mounted on the first matching block slide bar, a first top spring is arranged between the first matching block slide bar and the first matching block slide bar, the first matching block slide bar is slidably mounted on the first mounting block slide bar, a mounting block is mounted on the slide bar, and the lower end of the first matching block slide bar is connected with the adjusting component, and the connecting installation sleeve is fixedly installed with the installation sleeve A.
Furthermore, a circulating sleeve pipe convex edge is arranged on the circulating sleeve pipe II, the circulating sleeve pipe II is rotatably limited and arranged on the connecting and mounting sleeve through the circulating sleeve pipe convex edge, the sealing end cover II is rotatably arranged on the circulating sleeve pipe II, a plurality of circulating cavities II are arranged on the circulating sleeve pipe II, the inner ends of the circulating cavities II slide to form a circulating push plate II, the circulating push plate II is arranged at one end of the push plate slide rod II, the other end of the push plate slide rod II is provided with a slide rod matching hemisphere II, a push plate slide rod push spring II is sleeved on the push plate slide rod II, the push plate slide rod push spring II is arranged between the slide rod matching hemisphere II and the circulating sleeve pipe II, the push plate slide rod II is slidably connected with the circulating sleeve pipe II, a matching disc II is rotatably arranged on the circulating sleeve pipe II, the slide rod matching hemisphere II is in contact with the matching disc II, a matching disc slide groove II is arranged on the matching disc II, and a matching block II is slidably arranged at the inner end of the matching disc slide groove II, the lower end of the matching block II is provided with a matching block sliding rod II in a sliding mode, the matching block II and the matching block sliding rod II are provided with a top spring II, the matching block sliding rod II is connected with the mounting block sliding rod in a sliding mode, the matching block sliding rod II is connected with the adjusting assembly, and the matching bevel gear II is fixedly connected with the circulating sleeve II.
Furthermore, a plurality of first circulation chambers are circumferentially arranged along a central axis of the first circulation sleeve, and a plurality of second circulation chambers are circumferentially arranged along a central axis of the second circulation sleeve.
Further, the adjusting component comprises an adjusting bottom plate, a bottom plate bracket I, a bottom plate bracket II, a motor, an input bevel gear, a driving bevel gear, an adjusting disc I, an adjusting disc II, a middle end matching column, a waist groove I, a waist groove II, a connecting rod I, a connecting rod II, a driving slider I, a driving slider II, a manual rotating rod I, a manual rotating rod II, a positioning clamping groove I, a positioning clamping groove II, a positioning clamping rod I, a positioning clamping rod push spring, a positioning clamping rod sliding cavity and a positioning clamping rod II, wherein the mounting sleeve connecting rod is fixedly mounted with the adjusting bottom plate, the bottom plate bracket I and the bottom plate bracket II are mounted on the adjusting bottom plate, the bracket I is rotatably connected with the circulating sleeve pipe I, the bottom plate bracket II is rotatably connected with the circulating sleeve pipe II, the motor is mounted on the adjusting bottom plate, the input bevel gear is mounted on an output shaft of the motor, the input bevel gear is meshed with the driving bevel gear, the middle end matching column is mounted on the adjusting bottom plate, and the adjusting disc I and the adjusting disc II are rotatably matched with the middle end matching column, the driving bevel gear is sleeved on a first adjusting disk and a second adjusting disk, a first waist groove is arranged on the first adjusting disk, a first matching block sliding rod is slidably arranged at the inner end of the first waist groove, a second waist groove is arranged on the second adjusting disk, a second matching block sliding rod is slidably arranged in the second waist groove, a first adjusting disk hinge column is arranged on the first adjusting disk, one end of a first connecting rod is hingedly arranged on the first adjusting disk hinge column, the other end of the first connecting rod is hingedly arranged on a first driving sliding block, a first sliding groove is formed in an adjusting bottom plate, the first driving sliding block is slidably arranged in the first sliding groove, the first driving sliding block is in threaded fit connection with a first manual rotating rod, the manual rotating rod is rotatably arranged on the adjusting bottom plate, a first positioning clamping rod sliding cavity is formed in the adjusting bottom plate, a first positioning clamping rod is slidably arranged at the inner end of the first positioning clamping rod sliding cavity, a plurality of first positioning clamping grooves are formed in the first manual rotating rod, the first positioning clamping rod is matched with one of the plurality of positioning clamping grooves, the adjusting disk II is provided with an adjusting disk hinge post II, the adjusting disk hinge post II is hinged to one end of a connecting rod II, the other end of the connecting rod II is hinged to a driving sliding block II, a sliding groove II is formed in the adjusting bottom plate, the driving sliding block II is slidably mounted in the sliding groove II, the driving sliding block II is in threaded fit with a manual rotating rod II, the manual rotating rod II is rotatably mounted on the adjusting bottom plate, the inner end of a positioning clamping rod sliding cavity is slidably mounted with a positioning clamping rod II, the manual rotating rod II is provided with a plurality of positioning clamping grooves II, the positioning clamping rod II is matched with a plurality of positioning clamping grooves II, and driving bevel teeth are meshed with the matching bevel teeth I and the matching bevel teeth II for transmission.
The invention has the advantages that:
the circulating assembly drives the generated carbon dioxide in the high-temperature sleeve assembly to circulate, so that part of the carbon dioxide circularly drives the turbofan blades, the turbofan blades drive the blade shaft and drive the first bevel gear to move, the first bevel gear drives the second bevel gear and the inner end rotating sleeve to rotate, the inner end rotating sleeve drives the stirring rod to move through the connecting frame, and the stirring rod stirs calcium carbonate and calcium oxide to uniformly heat the calcium carbonate and the calcium oxide; meanwhile, the particles in the gas are removed through the filter medium in the communicating component, and the particles are rewashed and discharged back to continue to react; the communication assembly is used for blowing the generated carbon dioxide into the three-way sleeve, and the three-way sleeve is used for leading the carbon dioxide to enter the two stacked cell culture box assemblies through the first vent hole and the second vent hole through the external connecting pipe, so that the cells in the cell culture dish in the cell culture box assemblies can conveniently breathe; the technical problem of the problem of low generation efficiency caused by the fact that calcium oxide generated after industrial calcium carbonate is calcined at high temperature at present can influence heating of calcium carbonate is solved, and the problem that a carbon dioxide incubator needs to be additionally provided with a carbon dioxide gas cylinder is also solved.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is a schematic view of the overall structure of the present invention;
FIG. 4 is a schematic cross-sectional line position of FIG. 3;
FIG. 5 is a cross-sectional view of FIG. 3;
FIG. 6 is a schematic view of a high temperature sleeve assembly of the present invention;
FIG. 7 is a schematic view of the position of the cut line of FIG. 6;
FIG. 8 is a cross-sectional view J-J of FIG. 6;
FIG. 9 is a cross-sectional view K-K of FIG. 6;
FIG. 10 is a first schematic view of a communication assembly of the present invention;
FIG. 11 is a cross-sectional view of FIG. 10;
FIG. 12 is a second schematic view of a communication assembly according to the present invention;
FIG. 13 is a cross-sectional view of FIG. 12;
FIG. 14 is a partial enlarged view A of FIG. 13;
FIG. 15 is a schematic view of the construction of the circulation assembly of the present invention;
FIG. 16 is a schematic view of the position of the cut line of FIG. 15;
FIG. 17 is a cross-sectional view B-B of FIG. 15;
FIG. 18 is a cross-sectional view C-C of FIG. 15;
FIG. 19 is a schematic view of the adjustment assembly of the present invention;
FIG. 20 is a first sectional view of the first section of FIG. 19;
FIG. 21 is a cross-sectional view D-D of FIG. 19;
FIG. 22 is a cross-sectional view E-E of FIG. 19;
FIG. 23 is a cross-sectional view F-F of FIG. 19;
FIG. 24 is a sectional view G-G of FIG. 19;
FIG. 25 is a partial enlarged view A of FIG. 24;
FIG. 26 is a schematic view of a structure of an adjusting disk of the present invention;
FIG. 27 is a schematic structural view of a second adjustment plate of the present invention;
FIG. 28 is a second sectional view of FIG. 19;
FIG. 29 is a cross-sectional view H-H of FIG. 19;
FIG. 30 is a partial enlarged view B of FIG. 17;
FIG. 31 is a schematic view of the cell culture chamber assembly of the present invention;
FIG. 32 is a first cross-sectional view of FIG. 31;
FIG. 33 is a second sectional view of FIG. 31;
FIG. 34 is a cross-sectional view B-B of FIG. 32;
FIG. 35 is a cross-sectional view C-C of FIG. 33;
FIG. 36 is a schematic view of the structure of the case of the present invention;
FIG. 37 is a first schematic structural view of a hand-pulling case of the present invention;
FIG. 38 is a second schematic structural view of the hand-pulling case of the present invention;
FIG. 39 is a schematic view of a cassette according to the present invention;
the notation in the figure is: a high temperature sleeve assembly 1; an outer sleeve 1-1; 1-2 parts of a lower end cover; 1-3 of a plug; 1-4 through holes I; 1-5 through holes II; the inner end rotates the sleeve 1-6; connecting frames 1-7; 1-8 parts of a stirring rod; 1-9 of turbofan blades; 1-10 parts of a blade shaft; 1-11 parts of a first bevel gear; 1-12 of fan-shaped through holes; 1-13 parts of a second bevel gear; 1-14 of a limiting annular boss; a communication assembly 2; 2-1 of a communication sleeve; 2-2 of a connecting pipe I; 2-3 parts of a filtering sleeve; 2-4 parts of a second filtering sleeve; sealing end covers I2-5; 2-6 of a connecting pipe II; connecting pipe III 2-7; 2-8 parts of a second sealing end cover; connecting pipe IV 2-9; 2-10 parts of external connecting pipe; 2-11 parts of a connecting sleeve; 2-12 parts of a first spring; 2-13 parts of a sealing ring; a circulation component 3; a circulating sleeve I3-1; the convex edge of the sleeve is 3-1-1; matching with the first bevel gear 3-2; connecting the mounting sleeve 3-3; 3-4 of a mounting block; mounting a block sliding rod 3-5; 3-6 parts of a circulating sleeve II; the convex edge II of the sleeve is 3-6-1; matching with a second bevel gear 3-7; 3-8 parts of a first circulating chamber; 3-9 parts of a first circulating push plate; 3-10 parts of a first push plate sliding rod; the sliding rod is matched with the hemisphere I3-11; a push spring I of the push plate sliding rod is 3-12; 3-13 of a matching disc I; a first matching disc sliding groove 3-14; 3-15 parts of a matching block I; matching block sliding rods I are 3-16; a second circulating chamber 3-17; 3-18 parts of a circulating push plate II; 3-19 parts of a second push plate slide bar; the sliding rod is matched with the hemisphere II by 3-20; a second push spring 3-21 of the push plate slide rod; a second matching disc 3-22; a second matching disc sliding groove 3-23; 3-24 parts of a matching block II; a second matching block sliding rod 3-25; 3-26 of a first top spring; a second top spring 3-27; an installation sleeve A4; a sleeve connecting rod 5 is installed; an adjustment assembly 6; adjusting the bottom plate 6-1; the first chute is 6-1-1; the second chute is 6-1-2; a first bottom plate bracket 6-2; 6-3 of a second bottom plate bracket; 6-4 of a motor; inputting the bevel gear 6-5; driving bevel gears 6-6; 6-7 of a first adjusting disc; a first adjusting disc hinge column 6-7-1; 6-8 parts of a second adjusting plate; a second adjusting disc hinged column 6-8-1; 6-9 of a middle-end matching column; 6-10 parts of a first waist groove; 6-11 parts of a second waist groove; 6-12 parts of a first connecting rod; 6-13 parts of a second connecting rod; driving the first slide block 6-14; 6-15 parts of a second driving slide block; 6-16 parts of a manual rotating rod; a second manual rotating rod 6-17; 6-18 of a positioning clamping groove I; 6-19 of a positioning clamping groove II; 6-20 parts of a first positioning clamping rod; 6-21 of a positioning clamp rod push spring; 6-22 positioning clamping rod sliding cavities; 6-23 of a second positioning clamping rod; a base 7; a cell culture chamber assembly 8; a box body 8-1; a first vent hole 8-1-1; 8-1-2 of a vent hole II; pulling the box body 8-2 by hand; a display screen 8-2-1; a handle 8-2-2; mounting holes 8-2-3; 8-2-4 of a rectangular chute; 8-3 of a culture box; a groove 8-3-1; 8-3-2 rectangular vent holes; placing the groove 8-3-3; 8-3-4 of rollers; an outer end limit screw 8-4; a sealing sleeve 9; a three-way sleeve 10; an external connecting pipe 11; a high temperature sleeve assembly controller 12.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
As shown in fig. 1-2, a cell culture box with a self-drum carbon dioxide preparation device comprises a high temperature sleeve assembly 1, a base 7, a cell culture box assembly 8, a sealing sleeve 9, a three-way sleeve 10, two external connecting pipes 11 and a high temperature sleeve assembly controller 12, wherein the high temperature sleeve assembly 1 is arranged at the inner end of the sealing sleeve 9, the sealing sleeve 9 is arranged on the base 7, the sealing sleeve 9 is provided with the three-way sleeve 10 and is communicated with the three-way sleeve 9, the three-way sleeve 10 is provided with the two external connecting pipes 11 and is communicated with the three-way sleeve, the cell culture box assembly 8 is provided with two, the two cell culture box assemblies 8 are stacked and arranged on the base 7, the cell culture box assemblies 8 are connected and communicated with the corresponding external connecting pipes 11, the cell culture box comprises a communication assembly 2, a circulation assembly 3, an installation sleeve a4, an installation sleeve connecting rod 5 and an adjusting assembly 6, the high temperature sleeve assembly 1 is connected with the communication assembly 2, adjusting part 6 is connected with high temperature sleeve subassembly 1, intercommunication subassembly 2 links to each other with circulation subassembly 3, and circulation subassembly 3 is installed on adjusting part 6 through installation cover A4, installation cover connecting rod 5, and installation cover A4 links to each other with installation cover connecting rod 5 is fixed, and intercommunication subassembly 2 communicates with each other with tee bend sleeve pipe 10, so sets up, and the velocity of flow and the flow of the steerable tee bend sleeve pipe 10 carbon dioxide of high temperature sleeve subassembly controller 12.
Wherein, as shown in fig. 31-39, the cell culture box component 8 comprises a box body 8-1, a hand-pulled box body 8-2, a culture box 8-3 and an outer end limiting screw 8-4, the box body 8-1 is provided with a first vent hole 8-1-1 and a second vent hole 8-1-2, the first vent hole 8-1-1 is communicated with one external connecting pipe 11, the second vent hole 8-1-2 is communicated with the other external connecting pipe 11, the hand-pulled box body 8-2 is slidably arranged at the inner end of the box body 8-1, the hand-pulled box body 8-2 is provided with a plurality of rectangular chutes 8-2-4, the culture box 8-3 is rotatably provided with a plurality of rollers 8-3-4, the culture box 8-3 is slidably arranged in the rectangular chutes 8-2-4 through the plurality of rollers 8-3-4, the hand-pulling box body 8-2 is provided with a mounting hole 8-2-3, an outer end limiting screw 8-4 is arranged in the mounting hole 8-2-3 in a threaded fit manner and used for limiting and locking the hand-pulling box body 8-2, and the rectangular chute 8-2-4 is communicated with the corresponding mounting hole 8-2-3, so that the box body 8-1 can keep tightness; the cell culture dish can be conveniently taken and put by pulling the box body 8-2 by hand; the culture box 8-3 can be used for conveniently placing a culture dish.
As shown in fig. 31-37, a display screen 8-2-1 and a handle 8-2-2 for conveniently pulling the hand pulling case 8-2 are disposed on the hand pulling case 8-2.
As shown in FIGS. 31 to 37, the culture cassette 8-3 is provided with a groove 8-3-1 having a convex edge, and the culture cassette 8-3 is provided with a rectangular vent hole 8-3-2 for ventilation and a placing groove 8-3-3 for placing a cell culture dish.
As shown in fig. 3-5, further, the high temperature sleeve assembly 1 includes an outer sleeve 1-1, a lower end cap 1-2, an inner end rotating sleeve 1-6, a connecting frame 1-7, a stirring rod 1-8, a turbofan blade 1-9, a blade shaft 1-10, a first bevel gear 1-11, a second bevel gear 1-13 and a limiting annular boss 1-14, the lower end of the outer sleeve 1-1 is provided with the lower end cap 1-2, the inner end of the outer sleeve 1-1 is provided with the limiting annular boss 1-14, the limiting annular boss 1-14 is rotatably provided with the inner end rotating sleeve 1-6, the inner end of the inner end rotating sleeve 1-6 is provided with the connecting frame 1-7, the connecting frame 1-7 is provided with a plurality of stirring rods 1-8, the inner end rotating sleeve 1-6 is provided with the second bevel gear 1-13, the conical teeth II 1-13 are in meshed transmission with the conical teeth I1-11, the conical teeth I1-11 are installed at one ends of the blade shafts 1-10, the turbofan blades 1-9 are installed at the other ends of the blade shafts 1-10, the blade shafts 1-10 are rotatably installed with the outer sleeve 1-1, the outer sleeve 1-1 is connected with the communicating component 2, the adjusting component 6 is connected with the outer sleeve 1-1, the arrangement is such that carbon dioxide generated in the high-temperature sleeve component 1 is driven to circulate by the circulating component 3, a part of carbon dioxide circularly drives the turbofan blades 1-9, the blade shafts 1-10 are driven by the turbofan blades 1-9, the conical teeth I1-11 are driven to move, and the conical teeth II 1-13 and the inner end rotating sleeve 1-6 are driven to rotate by the conical teeth I1-11, the inner end rotating sleeve 1-6 drives the stirring rod 1-8 to move through the connecting frame 1-7, and the calcium carbonate and the calcium oxide are stirred through the stirring rod 1-8 to be heated uniformly; meanwhile, the particles in the gas are removed through the filter medium in the communication assembly 2, and the particles are rewashed and discharged back to continue the reaction.
As shown in fig. 6-9, a feeding port is formed in the outer sleeve 1-1, and a plug 1-3 is connected to the feeding port in a threaded manner, so that the air tightness of the outer sleeve 1-1 can be maintained conveniently through the plug 1-3; a plurality of stirring rods 1-8 are circumferentially arranged on the connecting frame 1-7 along the central axis of the outer sleeve 1-1 in an array manner; the high-temperature heating device is arranged on the lower end cover 1-2 and is suitable for all heating devices, and the high-temperature heating device arranged on the lower end cover 1-2 can be used for high-temperature calcination of the industrial calcium carbonate in the outer sleeve 1-1.
As shown in fig. 10-14, the communication component 2 includes a communication sleeve 2-1, a first connecting pipe 2-2, a first filtering sleeve 2-3, a second filtering sleeve 2-4, a first sealing end cap 2-5, a second connecting pipe 2-6, a third connecting pipe 2-7, a second sealing end cap 2-8, a fourth connecting pipe 2-9, an external connecting pipe 2-10, and a connecting sleeve 2-11, wherein one end of the communication sleeve 2-1 is mounted on the outer sleeve 1-1, the outer sleeve 1-1 is provided with a plurality of fan-shaped through holes 1-12, the communication sleeve 2-1 is communicated with the plurality of fan-shaped through holes 1-12, the other end of the communication sleeve 2-1 is mounted on the communication sleeve 2-1, the first filtering sleeve 2-3 is mounted on one end of the second filtering sleeve 2-4, the other end of the second filtering sleeve 2-4 is provided with the first sealing end cap 2-5, one end of a second connecting pipe 2-6 is arranged on a first sealing end cover 2-5, the other end of the second connecting pipe 2-6 is arranged on an outer sleeve 1-1, a first through hole 1-4 is formed in the outer sleeve 1-1, the second connecting pipe 2-6 is communicated with the first through hole 1-4, a first sealing end cover 2-5 is connected with a circulating component 3, one end of a third connecting pipe 2-7 is arranged on the outer sleeve 1-1, a second through hole 1-5 is formed in the outer sleeve 1-1, the second through hole 1-5 is communicated with a third connecting pipe 2-7, the other end of the third connecting pipe 2-7 is arranged on a second sealing end cover 2-8, the second sealing end cover 2-8 is connected with the circulating component 3, one end of a fourth connecting pipe 2-9 is arranged on the second sealing end cover 2-8, the other end of the fourth connecting pipe 2-9 is arranged on a second connecting sleeve 2-11, the connecting sleeve 2-11 is provided with an external connecting pipe 2-10 in a threaded fit manner, and the arrangement is such that a part of carbon dioxide flows into the connecting pipe II 2-6 through the through hole I1-4 and flows through the circulating component 3 under the circulating action of the circulating component 3, when passing through the filtering sleeve I2-3 and the filtering sleeve II 2-4, the particles in the carbon dioxide gas flow are adsorbed and recovered through the filtering medium between the filtering sleeve I2-3 and the filtering sleeve II 2-4, and meanwhile, the filtering sleeve I2-3 and the filtering sleeve II 2-4 are of a two-body structure, so that the filtering medium can be conveniently detached and replaced; meanwhile, carbon dioxide gas flows into the communication sleeve 2-1 through the connecting pipe I2-2 and flows into the outer sleeve 1-1 through the fan-shaped through holes 1-12, and simultaneously, the turbofan blades 1-9 are driven to move by the carbon dioxide gas.
As shown in figures 10-14, a sealing ring 2-13 is arranged in the connecting sleeve 2-11, and the sealing ring 2-13 is connected with the connecting sleeve 2-11 through a spring I2-12, so that the air tightness of the connecting sleeve 2-11 can be maintained through the sealing ring 2-13.
As shown in fig. 15-18 and 30, the circulating component 3 comprises a circulating sleeve I3-1, a matching bevel gear I3-2, a connecting and mounting sleeve 3-3, a mounting block 3-4, a mounting block sliding rod 3-5, a circulating sleeve II 3-6, a matching bevel gear II 3-7, a circulating chamber I3-8, a circulating push plate I3-9, a push plate sliding rod I3-10, a sliding rod matching hemisphere I3-11, a push plate sliding rod push spring I3-12, a matching disk I3-13, a matching disk sliding groove I3-14, a matching block I3-15, a matching block sliding rod I3-16, a circulating chamber II 3-17, a circulating push plate II 3-18, a push plate sliding rod II 3-19, a sliding rod matching hemisphere II 3-20, a push plate sliding rod push spring II 3-21, a circulating plate sliding rod push spring II 3-21, A second matching disc 3-22, a second matching disc sliding groove 3-23, a second matching block 3-24 and a second matching block sliding rod 3-25, wherein a first sealing end cover 2-5 is rotatably arranged on a first circulating sleeve 3-1, a first sleeve convex edge 3-1-1 is arranged on the first circulating sleeve 3-1, the first circulating sleeve 3-1 is rotatably limited and arranged on the first connecting and mounting sleeve 3-3 through the first sleeve convex edge 3-1-1, a first matching conical tooth 3-2 is arranged on the first circulating sleeve 3-1, a plurality of first circulating chambers 3-8 are arranged on the first circulating sleeve 3-1, a first circulating push plate 3-9 is slidably arranged at the inner end of the first circulating chamber 3-8, the first circulating push plate 3-9 is arranged at one end of the first sliding rod push plate 3-10, a first sliding rod matching hemisphere 3-11 is arranged at the other end of the first sliding rod 3-10, a push plate slide bar I3-10 is in sliding fit with a circulating sleeve I3-1, a push plate slide bar push spring I3-12 is sleeved on the push plate slide bar I3-10, a push plate slide bar push spring I3-12 is arranged between a slide bar matching hemisphere I3-11 and a circulating sleeve I3-1, a slide bar matching hemisphere I3-11 is attached to a matching disc I3-13, a matching disc I3-13 is rotatably installed with a connecting installation sleeve 3-3, a matching disc sliding groove I3-14 is formed on the matching disc I3-13, a matching block I3-15 is connected at the inner end of the matching disc sliding groove I3-14 in a sliding fit manner, as shown in figure 25, the matching block I3-15 is slidably installed on a matching block slide bar I3-16, and a top spring I3-26 is arranged between the matching block I3-15 and the matching block I3-16, the first matching block sliding rod 3-16 is slidably mounted on the second mounting block sliding rod 3-5, the mounting block 3-4 is mounted on the mounting block sliding rod 3-5, the lower end of the first matching block sliding rod 3-16 is connected with the adjusting assembly 6, and the connecting mounting sleeve 3-3 is fixedly mounted with a mounting sleeve A4; a circulating ring sleeve convex edge 3-6-1 is arranged on the circulating ring sleeve II 3-6, the circulating ring sleeve II 3-6 is rotatably limited and installed on the connecting and installing sleeve 3-3 through the circulating ring sleeve convex edge 3-6-1, a sealing end cover II 2-8 is rotatably installed on the circulating ring sleeve II 3-6, a plurality of circulating chambers II 3-17 are formed on the circulating sleeve II 3-6, a circulating push plate II 3-18 slides at the inner end of the circulating chamber II 3-17, the circulating push plate II 3-18 is installed at one end of a push plate slide rod II 3-19, the other end of the push plate slide rod II 3-19 is provided with a slide rod matching hemisphere II 3-20, a push plate slide rod push spring II 3-21 is sleeved on the push plate slide rod II 3-19, and the push plate slide rod push spring II 3-21 is arranged between the slide rod matching hemisphere II 3-20 and the circulating ring sleeve II 3-6, a second push plate sliding rod 3-19 is connected with a second circulating sleeve 3-6 in a sliding manner, a second matching disk 3-22 is rotatably arranged on the second circulating sleeve 3-6, a second sliding rod matching hemisphere 3-20 is contacted with the second matching disk 3-22, a second matching disk sliding groove 3-23 is arranged on the second matching disk 3-22, a second matching block 3-24 is slidably arranged at the inner end of the second matching disk sliding groove 3-23, as shown in figure 25, the lower end of the second matching block 3-24 is slidably arranged on the second matching block sliding rod 3-25, the second matching block 3-24 and the second matching block sliding rod 3-25 are provided with a second top spring 3-27, the second matching block sliding rod 3-25 is connected with a second mounting block sliding rod 3-5 in a sliding manner, the second matching block sliding rod 3-25 is connected with an adjusting component 6, and the second matching cone tooth 3-7 is fixedly connected with the second circulating sleeve 3-6, according to the arrangement, the first matching bevel gear 3-2 and the second matching bevel gear 3-7 are driven to rotate simultaneously by the output power of the adjusting component 6, the first circulating sleeve 3-1 is driven to move by the first matching bevel gear 3-2, the first circulating chamber 3-8 is driven to circulate by the first circulating sleeve 3-1, the first circulating push plate 3-9, the first push plate slide rod 3-10 and the first sliding rod matching hemisphere 3-11 are driven to simultaneously circulate by the first circulating chamber 3-8, and the first sliding rod matching hemisphere 3-11 slides along the first matching disk 3-13 under the pushing of the first push plate slide rod push spring 3-12, so that the first matching disk 3-13 is crossed with the central axis of the first connecting and mounting sleeve 3-3, and the first sliding rod matching hemisphere 3-11 performs circulating motion, the first circulation chamber 3-8 at the lower side and the first circulation push plate 3-9 therein form the largest space, the first circulation chamber 3-8 at the upper side and the first circulation push plate 3-9 therein form the smallest space, and the first circulation chamber 3-8 at the lower side and the first circulation push plate 3-9 therein form the smallest space, so that the first circulation chamber 3-8 and the first circulation push plate 3-9 therein form the space when the first circulation push plate 3-9 moves from bottom to top along with the circulation of the first circulation sleeve 3-1, the carbon dioxide gas flows, and then a part of the carbon dioxide flows into the second connection sleeve 2-6 through the first through hole 1-4, thereby completing the circulation of the gas; meanwhile, the circulating sleeve II 3-6 is driven to rotate along with the rotation of the matching bevel gear II 3-7, the circulating chamber II 3-17 is driven to move, the circulating chamber II 3-17 drives the circulating push plate II 3-18, the push plate slide bar II 3-19 and the slide bar matching hemisphere II 3-20 to perform circulating motion, the slide bar matching hemisphere II 3-20 slides along the matching disk II 3-22 under the pushing of the push plate slide bar push spring II 3-21, the matching disk II 3-22 is crossed with the central axis of the circulating sleeve I3-1, the matching disk II 3-22 and the matching disk I3-13 are inclined in the same direction, and the circulating push plate II 3-18 performs circulating motion and reciprocates along the axial direction of the circulating chamber II 3-17, meanwhile, the space formed by the second circulating chamber 3-17 positioned on the upper side of the device and the second circulating push plate 3-18 therein is the largest, and the space formed by the second circulating chamber 3-17 positioned on the lower side of the device and the second circulating push plate 3-18 therein is the smallest, so that the carbon dioxide in the outer sleeve 1-1 is pumped into the second circulating chamber 3-17 through the third connecting pipe 2-7, and the carbon dioxide is pushed out into the fourth connecting pipe 2-9 through the second circulating chamber 3-17 and flows out through the external connecting pipe 2-10, and the discharge of the carbon dioxide is completed; meanwhile, the inclination degree of the second matching discs 3-22 and the first matching discs 3-13 can be adjusted through the adjusting assembly 6, so that the difference value between the maximum value of the space formed by the first circulation chambers 3-8 at the lower side and the first circulation push plates 3-9 therein and the minimum value of the space formed by the first circulation chambers 3-8 at the upper side and the first circulation push plates 3-9 therein is changed, the difference value between the maximum value of the space formed by the second circulation chambers 3-17 at the upper side of the device and the second circulation push plates 3-18 therein and the minimum value of the space formed by the second circulation chambers 3-17 at the lower side of the device and the second circulation push plates 3-18 therein is changed, the single circulation amount of carbon dioxide is changed through the change of the space difference value, and the time length of the rotation of the turbofan blades 1-9 due to the airflow is changed, so that the stirring time of the stirring rods 1-8 for stirring the calcium carbonate and the calcium oxide is changed, and the stirring effect of the calcium carbonate and the calcium oxide is further adjusted; meanwhile, the amount of carbon dioxide flowing out through the external connecting pipes 2-10 in a single time is changed.
As shown in fig. 15-18, a plurality of the first circulation chambers 3-8 are circumferentially arranged along the central axis of the first circulation sleeve 3-1, and a plurality of the second circulation chambers 3-17 are circumferentially arranged along the central axis of the second circulation sleeve 3-6.
Wherein, as shown in fig. 19-29, the adjusting component 6 comprises an adjusting bottom plate 6-1, a bottom plate bracket I6-2, a bottom plate bracket II 6-3, a motor 6-4, an input bevel gear 6-5, a driving bevel gear 6-6, an adjusting disk I6-7, an adjusting disk II 6-8, a middle end matching column 6-9, a waist groove I6-10, a waist groove II 6-11, a connecting rod I6-12, a connecting rod II 6-13, a driving slider I6-14, a driving slider II 6-15, a manual rotating rod I6-16, a manual rotating rod II 6-17, a positioning clamping groove I6-18, a positioning clamping groove II 6-19, a positioning clamping rod I6-20, a positioning clamping rod push spring 6-21, a positioning clamping rod sliding cavity 6-22 and a positioning clamping rod II 6-23, the mounting sleeve connecting rod 5 is fixedly mounted with the adjusting bottom plate 6-1, the adjusting bottom plate 6-1 is provided with a bottom plate support I6-2 and a bottom plate support II 6-3, the support I6-2 is rotatably connected with the circulating sleeve I3-1, the bottom plate support II 6-3 is rotatably connected with the circulating sleeve II 3-6, the motor 6-4 is mounted on the adjusting bottom plate 6-1, the output shaft of the motor 6-4 is provided with an input bevel gear 6-5, the input bevel gear 6-5 is meshed with the driving bevel gear 6-6, the adjusting bottom plate 6-1 is provided with a middle end matching column 6-9, the adjusting plate I6-7 and the adjusting plate II 6-8 are rotatably matched with the middle end matching column 6-9, and the driving bevel gear 6-6 is sleeved on the adjusting plate I6-7 and the adjusting plate II 6-8, a first waist groove 6-10 is arranged on the first adjusting disk 6-7, a first matching block sliding rod 3-16 is slidably arranged at the inner end of the first waist groove 6-10, a second waist groove 6-11 is arranged on the second adjusting disk 6-8, a second matching block sliding rod 3-25 is slidably arranged in the second waist groove 6-11, a first adjusting disk hinge column 6-7-1 is arranged on the first adjusting disk 6-7, one end of a first connecting rod 6-12 is hingedly arranged on the first adjusting disk hinge column 6-7-1, the other end of the first connecting rod 6-12 is hingedly arranged on a first driving slide block 6-14, a first sliding groove 6-1-1 is arranged on the adjusting bottom plate 6-1, a first driving slide block 6-14 is slidably arranged in the first sliding groove 6-1-1, the first driving slide block 6-14 is in threaded fit connection with a first manual rotating rod 6-16, a first manual rotating rod 6-16 is rotatably arranged on an adjusting bottom plate 6-1, a first positioning clamping rod sliding cavity 6-22 is arranged on the adjusting bottom plate 6-1, a first positioning clamping rod 6-20 is slidably arranged at the inner end of the first positioning clamping rod sliding cavity 6-22, a plurality of first positioning clamping grooves 6-18 are arranged on the first manual rotating rod 6-16, the first positioning clamping rod 6-20 is matched with one of the first positioning clamping grooves 6-18, a second adjusting disk 6-8 is provided with a second adjusting disk hinge column 6-8-1, the second adjusting disk hinge column 6-8-1 is hinged and arranged at one end of a second connecting rod 6-13, the other end of the second connecting rod 6-13 is hinged and arranged on a second driving sliding block 6-15, a second sliding groove 6-1-2 is arranged on the adjusting bottom plate 6-1, the second driving sliding block 6-15 is slidably arranged in the second sliding groove 6-1-2, the driving sliding block II 6-15 is in threaded fit with the manual rotating rod II 6-17, the manual rotating rod II 6-17 is rotatably installed on the adjusting bottom plate 6-1, the inner end of the positioning clamping rod sliding cavity 6-22 is slidably provided with the positioning clamping rod II 6-23, the manual rotating rod II 6-17 is provided with a plurality of positioning clamping grooves II 6-19, the positioning clamping rod II 6-23 is matched with one of the positioning clamping grooves II 6-19, the driving bevel gear 6-6 is in meshing transmission with the matching bevel gear I3-2 and the matching bevel gear II 3-7, and the motor 6-4 is started and drives the input bevel gear 6-5 to rotate, and the matching bevel gear I3-2 and the matching bevel gear II 3-7 are driven by the driving bevel gear 6-6 to drive the matching bevel gear I3-2 and the matching bevel gear II 3-7 to output power; meanwhile, manually rotating a manual rotating rod I6-16 and driving a driving slide block I6-1 to slide in a sliding groove I6-1-1, so that the driving slide block I6-1 drives a connecting rod I6-12 and drives a regulating disc hinge column I6-7-1 to move, further drives a regulating disc I6-7 to move through the regulating disc hinge column I6-7-1, further drives a matching block I3-16 to slide along a mounting block slide rod 3-5 and drives a matching block I3-15 to move through the connection between a waist groove I6-10 and the matching block slide rod I3-16, so that the matching block I3-15 slides along a matching disc sliding groove I3-14 and further drives the matching disc I3-13 to move through the matching block I3-15, so that the inclination degree of the first matching disc 3-13 is changed; meanwhile, the second manual rotating rod 6-17 is manually rotated and drives the second driving slide block 6-15 to slide in the second sliding groove 6-1-2, the second connecting rod 6-13 is driven to move by the second driving sliding block 6-15, the second adjusting disk hinge column 6-8-1 is driven to move by the second connecting rod 6-13, and the second adjusting disk 6-8 is driven to move, thereby passing through the connection between the waist grooves two 6-11 and the matching block sliding bars two 3-25, so that the second matching block sliding rod 3-25 slides along the second mounting block sliding rod 3-5 and drives the second matching block 3-24 to move, so that the two matching blocks 3-24 move along the two matching disc sliding grooves 3-23, so that the inclination degree of the second matching discs 3-22 is changed, thereby completing the adjustment.
As shown in fig. 19 to 29, the driving bevel gear 6-6 and the middle-end fitting column 6-9 are coaxially arranged, a plurality of the first positioning clamping grooves 6-18 are circumferentially arrayed along the central axis of the first manual rotating rod 6-16, and a plurality of the second positioning clamping grooves 6-19 are circumferentially arrayed along the central axis of the second manual rotating rod 6-17.
The working principle is as follows:
the communication component 2 blows the generated carbon dioxide into the three-way sleeve 10, the three-way sleeve 10 leads the carbon dioxide into the two stacked cell culture box components 8 through the vent hole I8-1-1 and the vent hole II 8-1-2 through the external connecting pipe 11, and the cells in the cell culture dishes in the cell culture box components 8 can breathe conveniently;
the external connecting pipe 2-10 is externally connected with the three-way sleeve 10, the concentration of carbon dioxide in the air is low, and the cell culture medium needs carbon dioxide to adjust the pH value, so that the carbon dioxide generated by the external connecting pipe 2-10 of the incubator is maintained at 5 percent, the incubator is provided with a carbon dioxide preparation device, and a gas cylinder and a large number of external pipelines can be omitted; the circulating component 3 drives the carbon dioxide generated in the high-temperature sleeve component 1 to circulate, so that a part of the carbon dioxide circularly drives the turbofan blades 1-9, the turbofan blades 1-9 drive the blade shafts 1-10, the first bevel gear 1-11 is driven to move, the first bevel gear 1-11 drives the second bevel gear 1-13 and the inner end rotating sleeve 1-6 to rotate, the inner end rotating sleeve 1-6 drives the stirring rod 1-8 to move through the connecting frame 1-7, and the stirring rod 1-8 is used for stirring calcium carbonate and calcium oxide to uniformly heat the calcium carbonate and calcium oxide; meanwhile, the particles in the gas are removed through the filter medium in the communicating component 2, and the particles are rewashed and discharged back to continue the reaction; through the circulation effect of the circulation component 3, a part of carbon dioxide flows into the connecting pipe II 2-6 through the through hole I1-4 and flows through the circulation component 3, when the carbon dioxide passes through the filtering sleeve I2-3 and the filtering sleeve II 2-4, the particles in the carbon dioxide gas flow are adsorbed and recovered through the filtering medium between the filtering sleeve I2-3 and the filtering sleeve II 2-4, and meanwhile, the filtering sleeve I2-3 and the filtering sleeve II 2-4 are of a two-body structure, so that the filtering medium can be conveniently detached and replaced; meanwhile, carbon dioxide gas flows into the communication sleeve 2-1 through the first connecting pipe 2-2 and flows into the outer sleeve 1-1 through the fan-shaped through holes 1-12, and simultaneously, the turbofan blades 1-9 are driven to move by the carbon dioxide gas; the technical problem of low generation efficiency caused by the fact that calcium oxide generated after industrial calcium carbonate is calcined at high temperature can affect heating of calcium carbonate is solved; the first matching bevel gear 3-2 and the second matching bevel gear 3-7 are driven to rotate simultaneously by the output power of the adjusting component 6, the first matching bevel gear 3-2 drives the first circulating sleeve 3-1 to move, the first circulating sleeve 3-1 drives the first circulating chamber 3-8 to circulate, the first circulating chamber 3-8 drives the first circulating push plate 3-9, the first push plate slide bar 3-10 and the first sliding bar matching hemisphere 3-11 to simultaneously perform circulating motion, and simultaneously, under the pushing action of the first push plate slide bar push spring 3-12, the first sliding bar matching hemisphere 3-11 slides along the first matching disc 3-13, because the first matching disc 3-13 is crossed with the central axis of the connecting and mounting sleeve 3-3, the first sliding bar matching hemisphere 3-11 performs circulating motion, the first circulation chamber 3-8 at the lower side and the first circulation push plate 3-9 therein form the largest space, the first circulation chamber 3-8 at the upper side and the first circulation push plate 3-9 therein form the smallest space, and the first circulation chamber 3-8 at the lower side and the first circulation push plate 3-9 therein form the smallest space, so that the first circulation chamber 3-8 and the first circulation push plate 3-9 therein form the space when the first circulation push plate 3-9 moves from bottom to top along with the circulation of the first circulation sleeve 3-1, the carbon dioxide gas flows, and then a part of the carbon dioxide flows into the second connection sleeve 2-6 through the first through hole 1-4, thereby completing the circulation of the gas; meanwhile, the circulating sleeve II 3-6 is driven to rotate along with the rotation of the matching bevel gear II 3-7, the circulating chamber II 3-17 is driven to move, the circulating chamber II 3-17 drives the circulating push plate II 3-18, the push plate slide bar II 3-19 and the slide bar matching hemisphere II 3-20 to perform circulating motion, the slide bar matching hemisphere II 3-20 slides along the matching disk II 3-22 under the pushing of the push plate slide bar push spring II 3-21, the matching disk II 3-22 is crossed with the central axis of the circulating sleeve I3-1, the matching disk II 3-22 and the matching disk I3-13 are inclined in the same direction, and the circulating push plate II 3-18 performs circulating motion and reciprocates along the axial direction of the circulating chamber II 3-17, meanwhile, the space formed by the second circulating chamber 3-17 positioned on the upper side of the device and the second circulating push plate 3-18 therein is the largest, and the space formed by the second circulating chamber 3-17 positioned on the lower side of the device and the second circulating push plate 3-18 therein is the smallest, so that the carbon dioxide in the outer sleeve 1-1 is pumped into the second circulating chamber 3-17 through the third connecting pipe 2-7, and the carbon dioxide is pushed out into the fourth connecting pipe 2-9 through the second circulating chamber 3-17 and flows out through the external connecting pipe 2-10, and the discharge of the carbon dioxide is completed; meanwhile, the inclination degree of the second matching discs 3-22 and the first matching discs 3-13 can be adjusted through the adjusting assembly 6, so that the difference value between the maximum value of the space formed by the first circulation chambers 3-8 at the lower side and the first circulation push plates 3-9 therein and the minimum value of the space formed by the first circulation chambers 3-8 at the upper side and the first circulation push plates 3-9 therein is changed, the difference value between the maximum value of the space formed by the second circulation chambers 3-17 at the upper side of the device and the second circulation push plates 3-18 therein and the minimum value of the space formed by the second circulation chambers 3-17 at the lower side of the device and the second circulation push plates 3-18 therein is changed, the single circulation amount of carbon dioxide is changed through the change of the space difference value, and the time length of the rotation of the turbofan blades 1-9 due to the airflow is changed, so that the stirring time of the stirring rods 1-8 for stirring the calcium carbonate and the calcium oxide is changed, and the stirring effect of the calcium carbonate and the calcium oxide is further adjusted; meanwhile, the amount of carbon dioxide flowing out through the external connecting pipe 2-10 in a single time is changed; starting a motor 6-4, driving an input bevel gear 6-5 to rotate, driving a matching bevel gear I3-2 and a matching bevel gear II 3-7 through a driving bevel gear 6-6, and further outputting power; meanwhile, manually rotating a manual rotating rod I6-16 and driving a driving slide block I6-1 to slide in a sliding groove I6-1-1, so that the driving slide block I6-1 drives a connecting rod I6-12 and drives a regulating disc hinge column I6-7-1 to move, further drives a regulating disc I6-7 to move through the regulating disc hinge column I6-7-1, further drives a matching block I3-16 to slide along a mounting block slide rod 3-5 and drives a matching block I3-15 to move through the connection between a waist groove I6-10 and the matching block slide rod I3-16, so that the matching block I3-15 slides along a matching disc sliding groove I3-14 and further drives the matching disc I3-13 to move through the matching block I3-15, so that the inclination degree of the first matching disc 3-13 is changed; meanwhile, the second manual rotating rod 6-17 is manually rotated and drives the second driving slide block 6-15 to slide in the second sliding groove 6-1-2, the second connecting rod 6-13 is driven to move by the second driving sliding block 6-15, the second adjusting disk hinge column 6-8-1 is driven to move by the second connecting rod 6-13, and the second adjusting disk 6-8 is driven to move, and then the waist grooves 6-11 are connected with the matching block sliding bars 3-25, so that the second matching block sliding rod 3-25 slides along the second mounting block sliding rod 3-5 and drives the second matching block 3-24 to move, so that the two matching blocks 3-24 move along the two matching disc sliding grooves 3-23, so that the inclination degree of the second matching discs 3-22 is changed, thereby completing the adjustment.
It is to be understood that the present invention has been described with reference to certain embodiments, and that various changes in the features and embodiments, or equivalent substitutions may be made therein by those skilled in the art without departing from the spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation or material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the invention not be limited to the particular embodiment disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims (10)

1. The utility model provides a cell culture case with from drum-type carbon dioxide preparation facilities, includes high temperature sleeve subassembly (1), base (7), cell culture case subassembly (8), sealing sleeve pipe (9), tee bend sleeve pipe (10), external connecting pipe (11) and high temperature sleeve subassembly controller (12), install in sealing sleeve pipe (9) inner high temperature sleeve subassembly (1), and sealing sleeve pipe (9) are installed on base (7), install tee bend sleeve pipe (10) and intercommunication on sealing sleeve pipe (9), install two external connecting pipe (11) and intercommunication on tee bend sleeve pipe (10), cell culture case subassembly (8) are provided with two, two cell culture case subassembly (8) pile up and install on base (7), cell culture case subassembly (8) are connected and are linked through with corresponding external connecting pipe (11), its characterized in that: still include intercommunication subassembly (2), circulation subassembly (3), installation cover A (4), installation cover connecting rod (5) and adjusting part (6), high temperature sleeve subassembly (1) is connected with intercommunication subassembly (2), and adjusting part (6) are connected with high temperature sleeve subassembly (1), intercommunication subassembly (2) link to each other with circulation subassembly (3), and circulation subassembly (3) are installed on adjusting part (6) through installation cover A (4), installation cover connecting rod (5), and installation cover A (4) link to each other with installation cover connecting rod (5) is fixed, and intercommunication subassembly (2) communicate with each other with tee bend sleeve pipe (10).
2. A cell culture chamber with a self-drum carbon dioxide production device according to claim 1, characterized in that: the cell culture box assembly (8) comprises a box body (8-1), a hand-pulling box body (8-2), a culture box (8-3) and an outer end limiting screw rod (8-4), wherein the box body (8-1) is provided with a first vent hole (8-1-1) and a second vent hole (8-1-2), the first vent hole (8-1-1) is communicated with one external connecting pipe (11), the second vent hole (8-1-2) is communicated with the other external connecting pipe (11), the hand-pulling box body (8-2) is slidably arranged at the inner end of the box body (8-1), the hand-pulling box body (8-2) is provided with a plurality of rectangular sliding grooves (8-2-4), and a plurality of rollers (8-3-4) are rotatably arranged on the culture box (8-3), the culture box (8-3) is slidably mounted in a rectangular sliding groove (8-2-4) through a plurality of rollers (8-3-4), a mounting hole (8-2-3) is formed in the hand-pulling box body (8-2), an outer-end limiting screw rod (8-4) is mounted in the mounting hole (8-2-3) in a threaded fit mode and is used for limiting and locking the hand-pulling box body (8-2), and the rectangular sliding groove (8-2-4) is communicated with the corresponding mounting hole (8-2-3).
3. A cell culture chamber with a self-drum carbon dioxide production device according to claim 1, characterized in that: the high-temperature sleeve component (1) comprises an outer sleeve (1-1), a lower end cover (1-2), an inner end rotating sleeve (1-6), a connecting frame (1-7), stirring rods (1-8), turbofan blades (1-9), a blade shaft (1-10), a first bevel gear (1-11), a second bevel gear (1-13) and a limiting annular boss (1-14), wherein the lower end cover (1-2) is installed at the lower end of the outer sleeve (1-1), the limiting annular boss (1-14) is arranged at the inner end of the outer sleeve (1-1), the inner end rotating sleeve (1-6) is rotatably installed on the limiting annular boss (1-14), the connecting frame (1-7) is installed at the inner end rotating sleeve (1-6), and the plurality of stirring rods (1-8) are installed on the connecting frame (1-7), the inner end rotating sleeve (1-6) is provided with a second bevel gear (1-13), the second bevel gear (1-13) is in meshing transmission with the first bevel gear (1-11), the first bevel gear (1-11) is installed at one end of the blade shaft (1-10), the other end of the blade shaft (1-10) is provided with a turbofan blade (1-9), the blade shaft (1-10) is rotatably installed with the outer sleeve (1-1), the outer sleeve (1-1) is connected with the communicating component (2), and the adjusting component (6) is connected with the outer sleeve (1-1).
4. A cell culture chamber with a self-drum carbon dioxide production device according to claim 3, characterized in that the lower end cover (1-2) is provided with a high temperature heating device.
5. The cell culture box with the self-drum type carbon dioxide preparation device as claimed in claim 1 or 3, wherein the communication assembly (2) comprises a communication sleeve (2-1), a connecting pipe I (2-2), a filtering sleeve I (2-3), a filtering sleeve II (2-4), a sealing end cover I (2-5), a connecting pipe II (2-6), a connecting pipe III (2-7), a sealing end cover II (2-8), a connecting pipe IV (2-9), an external connecting pipe (2-10) and a connecting sleeve (2-11), one end of the communication sleeve (2-1) is arranged on the outer sleeve (1-1), the outer sleeve (1-1) is provided with a plurality of fan-shaped through holes (1-12), and the communication sleeve (2-1) is communicated with the plurality of fan-shaped through holes (1-12), the other end of the connecting sleeve (2-1) is arranged on the connecting pipe I (2-2), the filtering sleeve I (2-3) is arranged at one end of the filtering sleeve II (2-4), the other end of the filtering sleeve II (2-4) is provided with a sealing end cover I (2-5), one end of the connecting pipe II (2-6) is arranged on the sealing end cover I (2-5), the other end of the connecting pipe II (2-6) is arranged on the outer sleeve (1-1), the outer sleeve (1-1) is provided with a through hole I (1-4), the connecting pipe II (2-6) is communicated with the through hole I (1-4), the sealing end cover I (2-5) is connected with the circulating component (3), one end of the connecting pipe III (2-7) is arranged on the outer sleeve (1-1), the outer sleeve (1-1) is provided with a through hole II (1-5), the through hole II (1-5) is communicated with the connecting pipe III (2-7), the other end of the connecting pipe III (2-7) is installed on the sealing end cover II (2-8), the sealing end cover II (2-8) is connected with the circulating component (3), one end of the connecting pipe IV (2-9) is installed on the sealing end cover II (2-8), the other end of the connecting pipe IV (2-9) is installed on the connecting sleeve (2-11), and the connecting sleeve (2-11) is installed with an external connecting pipe (2-10) in a threaded fit mode.
6. The cell culture box with the self-drum type carbon dioxide preparation device as claimed in claim 5, wherein the connecting sleeve (2-11) is internally provided with a sealing ring (2-13), and the sealing ring (2-13) is connected with the connecting sleeve (2-11) through a spring I (2-12).
7. The cell culture box with the self-drum carbon dioxide preparation device as claimed in claim 1, wherein the circulating assembly (3) comprises a circulating sleeve I (3-1), a matching bevel gear I (3-2), a connecting and mounting sleeve (3-3), a mounting block (3-4), a mounting block sliding rod (3-5), a circulating sleeve II (3-6), a matching bevel gear II (3-7), a circulating chamber I (3-8), a circulating push plate I (3-9), a push plate sliding rod I (3-10), a sliding rod matching hemisphere I (3-11), a push plate sliding rod push spring I (3-12), a matching disc I (3-13), a matching disc sliding groove I (3-14), a matching block I (3-15), a matching block sliding rod I (3-16), A second circulating chamber (3-17), a second circulating push plate (3-18), a second push plate slide bar (3-19), a second slide bar matching hemisphere (3-20), a second push plate slide bar push spring (3-21), a second matching disc (3-22), a second matching disc slide groove (3-23), a second matching block (3-24) and a second matching block slide bar (3-25), wherein a first sealing end cover (2-5) is rotatably arranged on a first circulating sleeve (3-1), a first sleeve convex edge (3-1-1) is arranged on the first circulating sleeve (3-1), the first circulating sleeve (3-1) is rotatably limited and arranged on a first connecting and mounting sleeve (3-3) through a first sleeve convex edge (3-1-1), and a first matching conical tooth (3-2) is arranged on the first circulating sleeve (3-1), a plurality of first circulation chambers (3-8) are formed in the first circulation sleeve (3-1), a first circulation push plate (3-9) is slidably mounted at the inner end of the first circulation chamber (3-8), the first circulation push plate (3-9) is mounted at one end of the first push plate slide rod (3-10), a first slide rod matching hemisphere (3-11) is mounted at the other end of the first push plate slide rod (3-10), the first push plate slide rod (3-10) is slidably matched with the first circulation sleeve (3-1), a first push plate slide rod push spring (3-12) is sleeved on the first push plate slide rod (3-10), a first push plate slide rod push spring (3-12) is arranged between the first slide rod matching hemisphere (3-11) and the first circulation sleeve (3-1), and the first slide rod matching hemisphere (3-11) is attached to the first matching disc (3-13), the first matching disc (3-13) and the connecting and installing sleeve (3-3) are rotatably installed, a first matching disc sliding groove (3-14) is formed in the first matching disc (3-13), the inner end of the first matching disc sliding groove (3-14) is connected with a first matching block (3-15) in a sliding and matching mode, the first matching block (3-15) is installed on a first matching block sliding rod (3-16) in a sliding mode, and a first top spring (3-26) is arranged between the first matching block (3-15) and the first matching block sliding rod (3-16), the first matching block sliding rod (3-16) is slidably mounted on the first mounting block sliding rod (3-5), the mounting block (3-4) is mounted on the mounting block sliding rod (3-5), the lower end of the first matching block sliding rod (3-16) is connected with the adjusting component (6), and the mounting sleeve (3-3) and the mounting sleeve A (4) are fixedly mounted.
8. The cell culture box with the self-drum carbon dioxide preparation device as claimed in claim 7, wherein the second circulating sleeve (3-6) is provided with a second circulating sleeve convex edge (3-6-1), the second circulating sleeve (3-6) is rotatably and limitedly mounted on the connecting and mounting sleeve (3-3) through the second circulating sleeve convex edge (3-6-1), the second sealing end cap (2-8) is rotatably mounted on the second circulating sleeve (3-6), the second circulating sleeve (3-6) is provided with a plurality of second circulating chambers (3-17), the inner end of the second circulating chamber (3-17) slides on the second circulating push plate (3-18), the second circulating push plate (3-18) is mounted at one end of the second push plate slide bar (3-19), the other end of the second push plate slide bar (3-19) is provided with a second slide bar mating hemisphere (3-20), a second push plate slide bar (3-21) is sleeved on the second push plate slide bar (3-19), the second push plate slide bar push spring (3-21) is arranged between the second slide bar matching hemisphere (3-20) and the second circulating sleeve (3-6), the second push plate slide bar (3-19) is in sliding connection with the second circulating sleeve (3-6), a second matching disc (3-22) is rotatably installed on the second circulating sleeve (3-6), the second slide bar matching hemisphere (3-20) is in contact with the second matching disc (3-22), the second matching disc (3-22) is provided with a second matching disc slide groove (3-23), a second matching block (3-24) is slidably installed at the inner end of the second matching disc slide groove (3-23), and a second matching block (3-25) is slidably installed at the lower end of the second matching block (3-24), and the matching block II (3-24) and the matching block sliding rod II (3-25) are provided with a top spring II (3-27), the matching block sliding rod II (3-25) is connected with the mounting block sliding rod (3-5) in a sliding manner, the matching block sliding rod II (3-25) is connected with the adjusting component (6), and the matching bevel gear II (3-7) is fixedly connected with the circulating sleeve pipe II (3-6).
9. The cell incubator with the self-drum type carbon dioxide production apparatus according to claim 8, wherein a plurality of said circulation chambers one (3-8) are circumferentially arranged along a central axis of said circulation sleeve one (3-1), and a plurality of said circulation chambers two (3-17) are circumferentially arranged along a central axis of said circulation sleeve two (3-6).
10. The cell culture box with the self-drum carbon dioxide preparation device as claimed in claim 1, wherein the adjusting assembly (6) comprises an adjusting bottom plate (6-1), a first bottom plate support (6-2), a second bottom plate support (6-3), a motor (6-4), input bevel teeth (6-5), driving bevel teeth (6-6), a first adjusting disc (6-7), a second adjusting disc (6-8), a middle end matching column (6-9), a first waist groove (6-10), a second waist groove (6-11), a first connecting rod (6-12), a second connecting rod (6-13), a first driving slide block (6-14), a second driving slide block (6-15), a first manual rotating rod (6-16), a second manual rotating rod (6-17), a first positioning clamping groove (6-18), A second positioning clamping groove (6-19), a first positioning clamping rod (6-20), a second positioning clamping rod (6-21), a first positioning clamping rod push spring (6-21), a second positioning clamping rod sliding cavity (6-22) and a second positioning clamping rod (6-23), wherein the mounting sleeve connecting rod (5) is fixedly mounted with an adjusting bottom plate (6-1), a first bottom plate bracket (6-2) and a second bottom plate bracket (6-3) are mounted on the adjusting bottom plate (6-1), the first bracket (6-2) is rotatably connected with a first circulating sleeve (3-1), the second bottom plate bracket (6-3) is rotatably connected with a second circulating sleeve (3-6), a motor (6-4) is mounted on the adjusting bottom plate (6-1), an input bevel gear (6-5) is mounted on an output shaft of the motor (6-4), the input bevel gear (6-5) is meshed with the driving bevel gear (6-6), a middle-end matching column (6-9) is arranged on the adjusting bottom plate (6-1), the first adjusting plate (6-7) and the second adjusting plate (6-8) are in running fit with the middle-end matching column (6-9), a driving bevel gear (6-6) is sleeved on the first adjusting plate (6-7) and the second adjusting plate (6-8), a first waist groove (6-10) is arranged on the first adjusting plate (6-7), a first matching block sliding rod (3-16) is slidably arranged at the inner end of the first waist groove (6-10), a second waist groove (6-11) is arranged on the second adjusting plate (6-8), a second matching block sliding rod (3-25) is slidably arranged in the second waist groove (6-11), a first adjusting plate hinge column (6-7-1) is arranged on the first adjusting plate (6-7), one end of a first connecting rod (6-12) is hinged on a hinge column (6-7-1) of an adjusting disc, the other end of the first connecting rod (6-12) is hinged on a first driving sliding block (6-14), a first sliding groove (6-1-1) is formed in an adjusting bottom plate (6-1), the first driving sliding block (6-14) is slidably arranged in the first sliding groove (6-1-1), the first driving sliding block (6-14) is in threaded fit connection with a first manual rotating rod (6-16), the first manual rotating rod (6-16) is rotatably arranged on the adjusting bottom plate (6-1), a first positioning clamping rod sliding cavity (6-22) is formed in the adjusting bottom plate (6-1), and a first positioning clamping rod (6-20) is slidably arranged at the inner end of the first positioning clamping rod sliding cavity (6-22), a plurality of first positioning clamping grooves (6-18) are formed in the first manual rotating rod (6-16), the first positioning clamping rods (6-20) are matched with one of the first positioning clamping grooves (6-18), a second adjusting disk hinge column (6-8-1) is arranged on the second adjusting disk (6-8), the second adjusting disk hinge column (6-8-1) is hinged to one end of the second connecting rod (6-13), the other end of the second connecting rod (6-13) is hinged to the second driving sliding block (6-15), a second sliding groove (6-1-2) is formed in the adjusting bottom plate (6-1), the second driving sliding block (6-15) is slidably arranged in the second sliding groove (6-1-2), and the second driving sliding block (6-15) is in threaded fit with the second manual rotating rod (6-17), the manual rotating rod II (6-17) is rotatably installed on the adjusting bottom plate (6-1), the inner end of the positioning clamping rod sliding cavity (6-22) is provided with the positioning clamping rod II (6-23) in a sliding mode, the manual rotating rod II (6-17) is provided with a plurality of positioning clamping grooves II (6-19), the positioning clamping rod II (6-23) is matched with one of the positioning clamping grooves II (6-19), and the driving bevel gear (6-6) is in meshing transmission with the matching bevel gear I (3-2) and the matching bevel gear II (3-7).
CN202210450800.8A 2022-04-27 2022-04-27 Cell culture case with from drum-type carbon dioxide preparation facilities Active CN114561292B (en)

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1600685A (en) * 1998-03-13 2005-03-30 财团法人地球环境产业技术研究机构 Carbon producing apparatus utilizing biomass
CN101091444A (en) * 2007-07-20 2007-12-26 浙江大学 Apparatus capable of controlling density of CO2 for culturing growth of plant
US20110223582A1 (en) * 2008-11-13 2011-09-15 Castillo Jose Cell culture device and method of culturing cells
CN107721218A (en) * 2017-10-18 2018-02-23 中国科学院过程工程研究所 One kind utilizes magnesite coproduction binder materials and CO2Preparation method and equipment
CN207566889U (en) * 2017-12-05 2018-07-03 天津永利食用添加剂有限公司 A kind of preparation facilities of food-grade carbon-dioxide
CN108394901A (en) * 2018-07-09 2018-08-14 天津长和生物技术有限公司 The double set supply equipments of carbon dioxide
CN208454834U (en) * 2018-07-04 2019-02-01 浙江钙科机械设备有限公司 A kind of industrial calcium oxide equipment of high de-agglomeration rate high activity degree
US20190331364A1 (en) * 2016-12-09 2019-10-31 Nanjing Tech University Method and system of high-temperature calcium looping thermochemical energy storage
CN111772021A (en) * 2020-08-25 2020-10-16 广东汕泰食品有限公司 Carbon dioxide feeding device is used in jumping candy production
CN214327755U (en) * 2021-01-26 2021-10-01 安徽九川生物科技有限公司 Carbon dioxide incubator of PK cell culture

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1600685A (en) * 1998-03-13 2005-03-30 财团法人地球环境产业技术研究机构 Carbon producing apparatus utilizing biomass
CN101091444A (en) * 2007-07-20 2007-12-26 浙江大学 Apparatus capable of controlling density of CO2 for culturing growth of plant
US20110223582A1 (en) * 2008-11-13 2011-09-15 Castillo Jose Cell culture device and method of culturing cells
US20190331364A1 (en) * 2016-12-09 2019-10-31 Nanjing Tech University Method and system of high-temperature calcium looping thermochemical energy storage
CN107721218A (en) * 2017-10-18 2018-02-23 中国科学院过程工程研究所 One kind utilizes magnesite coproduction binder materials and CO2Preparation method and equipment
CN207566889U (en) * 2017-12-05 2018-07-03 天津永利食用添加剂有限公司 A kind of preparation facilities of food-grade carbon-dioxide
CN208454834U (en) * 2018-07-04 2019-02-01 浙江钙科机械设备有限公司 A kind of industrial calcium oxide equipment of high de-agglomeration rate high activity degree
CN108394901A (en) * 2018-07-09 2018-08-14 天津长和生物技术有限公司 The double set supply equipments of carbon dioxide
CN111772021A (en) * 2020-08-25 2020-10-16 广东汕泰食品有限公司 Carbon dioxide feeding device is used in jumping candy production
CN214327755U (en) * 2021-01-26 2021-10-01 安徽九川生物科技有限公司 Carbon dioxide incubator of PK cell culture

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