CN101085310B - Traditional Chinese medicine ointment for treating cow mastitis and method for preparing same - Google Patents

Abstract

The invention relates to a Chinese medicinal ointment for treating cow's mammitis through transdermal administration and process for preparation, wherein the matrix of the grease comprises the following raw materials (by weight ratio): coptis root 62.5-71.45%, oil of aromatic turmeric 9.5-18.75%, borneol 18.75-19.05%. The preparation consists of subjecting coptis root to water grilling and alcohol dissolving coptis root extract, dissolving borneol and oil of aromatic turmeric in 95% ethanol, heating wool grease, charging glycerol, charging alcoholic solution of borneol and oil of aromatic turmeric, coptis root extract into wool grease miscible liquid in sequence, stirring and split charging.

Description

Traditional Chinese herb medicine of treatment mammitis of cow and preparation method thereof

Technical field

The present invention relates to Chinese medicine composition preparation capable of permeating skin field, more particularly, relate to a kind of traditional Chinese herb medicine that is applicable to the treatment of milch cow clinic mastitis and preparation method thereof.

Background technology

Mastitis is one of important diseases of milch cow, often causes milk yield to descend even stops, and economic loss is serious.Antibiotic is the choice drug of treatment mammitis of cow, because the residue problem of antibiotic drug resistance and medicine becomes increasingly conspicuous, domestic in recent years some Chinese herbal and crude drugs preparationses of also having developed have curative effect preferably to mammitis of cow.Administrations such as that the treatment of mammitis of cow is taked more is oral, intramuscular injection, the perfusion of newborn chamber.Oral administration dosage is big, and medicine is easily destroyed at gastrointestinal tract.Intramuscular injection has " first pass effect of hepar ", and bioavailability is low, and some drugs is difficult to influence drug effect by " blood breast barrier ".Breast chamber perfusion administration is insoluble problem to the stimulation of mammary gland tissue, medicine to the influence of fresh milk quality etc. to the mechanical damage of nipple, medicine.By percutaneous drug delivery, can effectively avoid the defective of above-mentioned administering mode, give full play to the advantage that herbal toxic effect is little, be difficult for producing drug resistance, drug residue free, and administration number of times is few, clinical easy to use, be a kind of ideal administering mode.

Summary of the invention

Technical problem to be solved by this invention is, overcomes the deficiencies in the prior art, and a kind of traditional Chinese herb medicine through the transdermal medicine for treating mammitis of cow and preparation method thereof is provided.

The present invention treats the traditional Chinese herb medicine of mammitis of cow, contains the following weight proportion raw material in the mastic substrate: Rhizoma Coptidis 62.5-71.45%, Oleum Curcumae 9.5-18.75%, Borneolum Syntheticum 18.75-19.05%.

Substrate of the present invention is lanoline, glycerol, ethanol.

The present invention treats the preparation method of the traditional Chinese herb medicine of mammitis of cow, carries out according to following steps:

(1) Rhizoma Coptidis with proportioning described in the above-mentioned prescription breaks into coarse powder, adds water 3000ml and soaks 4h, decocts 2h, filters; Filtering residue adds water 2000ml, decocts 1.5h, filters; Filtering residue boils 1.5h with the 2000ml decocting again, filters; Merge 3 times filtrate, be concentrated into 600ml; Add 3 times of amount 95% ethanol, staticly settle 24h, filter; Filtrate recycling ethanol is concentrated into 500ml, gets Rhizoma Coptidis extract, and is standby.

(2) Borneolum Syntheticum and the Oleum Curcumae with proportioning described in the above-mentioned prescription is dissolved in 220ml 95% ethanol, gets Borneolum Syntheticum-Oleum Curcumae alcoholic solution, and be standby.

(3) lanoline 1800g is heated to 100 ℃ of 60min sterilizations, waits temperature, add glycerol 300ml to 40-50 ℃, mixing, insulation, standby.

(4) in step (3) lanoline mixed liquor, add the alcoholic solution of the Borneolum Syntheticum-Oleum Curcumae described in the step (2), stir 10min and make evenly, add the Rhizoma Coptidis extract described in the step (1) again, stir 15min and make emulsifying complete, become to spare the matter emulsifiable paste; Packing, promptly.

In the present invention prescription with Rhizoma Coptidis heat clearing and damp drying, anti-inflammation; The circulation of qi promoting of Oleum Curcumae removing blood stasis, blood stasis-eliminating and stagnation-dissipating; The Borneolum Syntheticum refreshment of having one's ideas straightened out, promoting flow of QI and blood, the stagnated fire of loosing, reducing swelling and alleviating pain, antimicrobial antiphlogistic promotes drug transdermal to absorb.Full side's function heat-clearing and toxic substances removing, anti-inflammation, circulation of qi promoting removing blood stasis with potent drugs, eliminating stagnation, pain relieving.Be applicable to the treatment of milch cow clinic mastitis.

Modern pharmacology studies show that Rhizoma Coptidis has extensively and significant resisting pathogenic microbes effect.The experiment of the Rhizoma Coptidis decocting liquid Plating of 100% concentration to 5 kinds of 7 kinds of gram negative bacterias such as dysentery bacterium, Bacillus typhi, Salmonella paratyphi, vibrio cholera, escherichia coli, Bacillus proteus and staphylococcus, Streptococcus hemolyticus, Diplococcus pneumoniae, bordetella pertussis etc. altogether the He Lanshi positive bacterias stronger bacteriostasis is all arranged.The decocting liquid of 8-35% concentration has inhibitory action to multiple town bacterium such as the little sporeformer of Canis familiaris L., Candida albicans.The Rhizoma Coptidis decoct has inhibitory action to first, second, influenza virus C, hepatitis B virus, trachoma virus.

1: 3200 concentration of the effective ingredient berberine of Rhizoma Coptidis has inhibitory action to Hemolytic streptococcus, Diplococcus pneumoniae, anthrax bacillus, vibrio cholera, concentration had inhibitory action to staphylococcus aureus, Staphylococcus albus in 1: 16000,1: 8000 pair of Staphylococcus citreus, viridans streptococci, diphtheria corynebacterium, bacillus subtilis, dysentery bacterium, shigella flexneri have inhibitory action, 1: 4000 pair of Bacillus typhi has inhibitory action, and 1: 1000 pair of Bacillus typhi has inhibitory action.

Rhizoma Coptidis decoct and berberine be external anti-ameba effect, in the body ameba is had killing action.

Rhizoma Coptidis has antiinflammatory action.The methanolic extract of Rhizoma Coptidis can suppress granulation tissue hyperplasia, and berberine hydrochloride also shows antiinflammatory action to rat Fructus Crotonis oil sac method, cotton pellet method.

Rhizoma Coptidis also has refrigeration function.Heat pyrexia has refrigeration function to the Rhizoma Coptidis injection to the rabbit leukocytic pyrogen, and its refrigeration function generates relevant with maincenter CAMP.

Rhizoma Coptidis extract all has good antibacterial action to mammitis of cow pathogen such as staphylococcus aureus, streptococcus, escherichia coli and yeast class fungus etc. among the present invention.The water extract-alcohol precipitation liquid of 1g crude drug/ml concentration is 20mm to golden Portugal bacterium antibacterial circle diameter, and streptococcus is 18.5mm, and yeast class fungus is 31mm.MIC and MBC are respectively: escherichia coli 1.56% and 6.25%, streptococcus 0.05% and 0.1%, golden Portugal bacterium 3.13% and 6.25%, yeast class fungus 0.024% and 0.1%.

Oleum Curcumae has antiinflammatory action, can suppress animal acetic acid peritonitis, and mice local edema, inflammation and rat granuloma are swollen the obvious treatment effect.

Curcumenol has function of promoting blood circulation to disperse blood clots to blood stasis model rat, normal mouse, and blood stasis model rat whole blood viscosity height, middle shearing are significantly reduced, and can also obviously prolong the mice blood clotting time.

One of Oleum Curcumae effective ingredient curcumenol can suppress the growth of staphylococcus aureus, beta hemolytic streptococcus, escherichia coli, Bacillus typhi etc. in vitro.(RSV) has direct repression to respiratory syncytial virus, to influenza virus A ₁, A ₃Type has direct deactivation.In addition, still can directly act on as rheumatism virus, chickenpox virus other virus.

The Oleum Curcumae prolonged application does not have harm to main organs, has no drug resistance, and untoward reaction is few, is low toxicity, safe drugs.

Borneolum Syntheticum all has antibacterial action external to staphylococcus aureus, beta hemolysis streptococcus, streptococcus pneumoniae, viridans streptococci and escherichia coli, and antibacterial mode is antibacterial for contact, and bacteriostasis is arranged when low concentration, and bactericidal action is arranged during high concentration; Antibacterial strength strengthens with prolonging time of contact.The active ingredient isoborneol Emulsion of Borneolum Syntheticum has remarkable inhibitory action to the mouse knoting oil auricle edema, and simultaneously, swelling has equal significant inhibitory effect to rat Ovum Gallus domesticus album toes for Borneolum Syntheticum and isoborneol Emulsion.

Borneolum Syntheticum can promote the interior absorption of the body of medicine.Its effective ingredient Borneolum Syntheticum and isoborneol are irritated the absorption that stomach can promote the main effective ingredient tetramethylpyazine of Chinese medicine Rhizoma Chuanxiong, improve its blood drug level.After share, Borneolum Syntheticum and nitrendipine can promote that the latter absorbs at gastrointestinal.Borneolum Syntheticum can significantly improve the intravital bioavailability of rifampicin rat, obviously improves the absorption of medicine.

Borneolum Syntheticum has short Transdermal absorption effect.Borneolum Syntheticum can promote the Transdermal absorption of medicines such as external 17-hydroxy-11-dehydrocorticosterone (triamcinolone acetonide), diclofenac, metronidazole, fluorouracil, salicylic acid, ligustrazine, triamcinolone acetonide acetate.The facilitation of discovering Borneolum Syntheticum may be because Borneolum Syntheticum changes the arrangement of lipid molecular and increases its flowability mainly at horny layer.Borneolum Syntheticum can also increase the storage storehouse effect of intact skin and destratum corneum skin thereof in addition.In stripped Periostracum Serpentis Transdermal absorption test, the Transdermal absorption of Borneolum Syntheticum increase metronidazole, fluorouracil urgees to ooze intensity and concentration is proportionate.Rabbit in body test, Borneolum Syntheticum can obviously increase the transdermal amount of salicylic acid and diclofenac, in the skin heart shrinkage test of human body, Borneolum Syntheticum itself does not cause the palor reaction, but can obviously strengthen the palor reaction of triamcinolone acetonide acetate, this effect of Borneolum Syntheticum can be used for improving the curative effect of many medicine for external use.Aspect the Transdermal absorption of Chinese medicine, after the HPLC detection method was found Radix Angelicae Sinensis, Borneolum Syntheticum compatibility, the infiltration coefficient of the contained ferulic acid of Radix Angelicae Sinensis increased.Adopt the increase along with Borneolum Syntheticum concentration of studies confirm that of two Room diffusion cell experiment in vitro devices, the infiltration coefficient of ligustrazine hydrochloride increases gradually, and after removing horny layer, Borneolum Syntheticum can not be urged.Think the short transdermal effect of Borneolum Syntheticum mainly at horny layer, possible mechanism is the Borneolum Syntheticum arrangement that can change lipid molecular, increases its flowability.

Borneolum Syntheticum can promote the nasal absorption of ligustrazine, and Borneolum Syntheticum concentration is at 1.0gL ^-1The time, the absorption rate of ligustrazine is the highest.The Borneolum Syntheticum of 0.12% concentration can significantly promote the absorption of the plain saturating oral mucosa in film island.

Borneolum Syntheticum to the Transdermal absorption facilitation of diclofenac and azone relatively, its transdermal enhancing effect a little less than, but lag time is shorter than azone, needs the short time just can reach active drug concentration.

Borneolum Syntheticum has good antiinflammatory and analgesic effect.Cavia porcellus is caused animal model of burn with laser, use Borneolum Syntheticum, JINGWANHONG, normal saline respectively, found that Borneolum Syntheticum treated animal pain threshold is high 6.24 and 2.78 times respectively than matched group and JINGWANHONG group; Vascular reaction, edema and inflammatory reaction are light than other two groups all.

The Borneolum Syntheticum safety in utilization is better.Experimental result shows, the oral Borneolum Syntheticum LD of rat ₅₀Be 2000mg/kg.Borneolum Syntheticum active ingredient Borneolum Syntheticum, isoborneol and composite are given the oral LD of mice ₅₀Be respectively 2879,2269,2507mg/kg.Genetic toxicology studies show that natural Broneolum Syntheticum and synthetic borneol Ames experimental result are all negative, points out two kinds of Borneolum Syntheticums all not have obvious mutagenic action.Mammal cultured cell (CHL) the chromosomal aberration experimental result of natural Broneolum Syntheticum and synthetic borneol is all negative, does not all have the DNA damage effect.

The specific embodiment

Below in conjunction with embodiment the present invention is described further.

The present invention carries out proportioning according to each embodiment in the following table:

	Rhizoma Coptidis (Rhizoma Coptidis extract)	Oleum Curcumae	Borneolum Syntheticum
				Embodiment 1	300g(500ml)	90g	90g
Embodiment 2	350g(500ml)	80g	100g
				Embodiment 3	400g(500ml)	70g	110g
Embodiment 4	450g(500ml)	60g	120g

With embodiment 1 in detail preparation method is described in detail:

(1) Rhizoma Coptidis 300g is broken into coarse powder, add water 3000ml and soak 4h, decoct 2h, filter; Filtering residue adds water 2000ml, decocts 1.5h, filters.Filtering residue boils 1.5h with the 2000ml decocting again, filters; Merge 3 times filtrate, be concentrated into 600ml; Add 3 times of amount 95% ethanol, staticly settle 24h, filter.Filtrate recycling ethanol is concentrated into 500ml, gets Rhizoma Coptidis extract, and is standby.

(2) Borneolum Syntheticum 90g, Oleum Curcumae 90g are dissolved in 220ml 95% ethanol, get Borneolum Syntheticum-Oleum Curcumae alcoholic solution, standby.

(3) lanoline 1800g is heated to 100 ℃ of 60min sterilizations, waits temperature, add glycerol 300ml to 40-50 ℃, mixing, insulation, standby.

(4) in above-mentioned lanoline mixed liquor, add the alcoholic solution of Borneolum Syntheticum-Oleum Curcumae, stir 10min and make evenly, add Rhizoma Coptidis extract again, stir 15min and make emulsifying complete, become to spare the matter emulsifiable paste; Packing, promptly.

Clinical efficacy of the present invention: the peaceful ointment of mastitis every day is applied to cow breast skin 2 times, and the about 1-2mm of thickness covers whole breast district degree of being, medication 5 days with ointment.Treat 31 37 ill breasts of ill milch cow district altogether, wherein acute mastitis breast district is 24, cures 14,5 of produce effects, effective 2, invalid 3, total effective rate 87.5%; 8 in subacute mastitis breast district cures 2,3 of produce effects, effective 1, invalid 2, total effective rate 75%; 5 in chronic mammitis breast district, 1 of produce effects, effective 2, invalid 2, total effective rate 60%.

Claims (3)

1. a traditional Chinese herb medicine for the treatment of mammitis of cow is characterized in that, the active ingredient in the described mastic substrate is by the following weight proportioning: Rhizoma Coptidis 62.5-71.45%, Oleum Curcumae 9.5-18.75%, Borneolum Syntheticum 18.75-19.05% make.

2. the traditional Chinese herb medicine of treatment mammitis of cow according to claim 1 is characterized in that, described mastic substrate is lanoline, glycerol, ethanol.

3. a preparation method for the treatment of the traditional Chinese herb medicine of mammitis of cow is characterized in that, carries out according to following steps:

(1) Rhizoma Coptidis with proportioning described in the claim 1 breaks into coarse powder, adds water 3000ml and soaks 4h, decocts 2h, filters; Filtering residue adds water 2000ml, decocts 1.5h, filters.Filtering residue boils 1.5h with the 2000ml decocting again, filters; Merge 3 times filtrate, be concentrated into 600ml; Add 3 times of amount 95% ethanol, staticly settle 24h, filter; Filtrate recycling ethanol is concentrated into 500ml, gets Rhizoma Coptidis extract, and is standby;

(2) Borneolum Syntheticum and the Oleum Curcumae with proportioning described in the claim 1 is dissolved in 220ml 95% ethanol, gets Borneolum Syntheticum-Oleum Curcumae alcoholic solution, and be standby;

(3) lanoline 1800g is heated to 100 ℃ of 60min sterilizations, waits temperature, add glycerol 300ml to 40-50 ℃, mixing, insulation, standby;

(4) in step (3) lanoline mixed liquor, add the alcoholic solution of the Borneolum Syntheticum-Oleum Curcumae described in the step (2), stir 10min and make evenly, add the Rhizoma Coptidis extract described in the step (1) again, stir 15min and make emulsifying complete, become to spare the matter emulsifiable paste; Packing, promptly.