CN101050228A - Method for constructing complicated Nano form by suing DNA molecule - Google Patents
Method for constructing complicated Nano form by suing DNA molecule Download PDFInfo
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- CN101050228A CN101050228A CNA2007100382970A CN200710038297A CN101050228A CN 101050228 A CN101050228 A CN 101050228A CN A2007100382970 A CNA2007100382970 A CN A2007100382970A CN 200710038297 A CN200710038297 A CN 200710038297A CN 101050228 A CN101050228 A CN 101050228A
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Abstract
This invention relates to a method for constructing complex nanostructure with DNA molecules. The method comprises: selecting a single-stranded DNA as a scaffold chain, horizontally folding the DNA to obtain targeted structure, designing several short single-stranded DNAs as staple chains according to the folded scaffold chain, mixing the staple chains and the scaffold chain in solution, annealing, and self-assembling to obtain the target structure. This invention also discloses a method for metallization of DNA nanostructure with electronic device shape in the solution, and transferring it into air to obtain nanoscale electronic device. The method has such advantages as easy operation, and can construct symmetric or asymmetric highly complex DNA nanostructure that can be used in electronic industry.
Description
Technical field
The present invention relates to a kind of method, thereby and the complicated nanometer shape of the DNA in the solution further is converted into other materials such as metal and is transferred to and realize the purposes of this method in the air at aspects such as electron device manufacturings with dna molecular complex structure nanometer shape (two-dimensional symmetric nanostructure, two-dimentional asymmetric nanostructure).The graphical manufacture field that belongs to nanotechnology.
Background technology
Than traditional top-down shape building method, self-assembling method from bottom to top is undoubtedly important novel texture (particularly nanoscale structures) manufacture method.The knowledge and technology accumulation of DNA and the code capacity of DNA self make the self-assembly building method based on DNA become the most potential self-assembling method.
The achievement in research of constructing nano-scale pattern with the DNA self-assembly emerges in an endless stream always.1989, Seeman has proposed elementary cell (the Seeman NC.Nanoscaleassembly and manipulation of branched DNA:A biological starting pointfor nanotechnology.Lewis J of a kind of cruciform as the DNA self-assembly first, Quel JL, eds.Nanocon Proceedings, NANOCON, P.O.Box 40176, Bellevue, WA 98004,1989.101-107).Afterwards, people such as Yan are further perfect with this cruciform, be self-assembled into grid, and observe grid structure (Yan H clearly with AFM, Reif JH, LaBean TH.DNA-templated self-assembly of protein arraysand highly conductive nanowires.Science, 2003,301:1882-1884).1998, people such as Winfree construct the self-assembly unit that the DNA chain is wound in two rows, be called the DX module, each DX module all has 4 cohesive terminuss, can be self-assembled into two-dimensional array (Winfree E, Liu F, SeemanNC.et al.Design and self-assembly of two-dimensional DNA crystals.Nature, 1998,394:539-544).The DX module can be self-assembled into trilateral (Rothemund PW by design, Papadakis N, Winfree E.Algorithmic self-assembly of DNASierpinski triangles.PLoS Biol, 2004,2:2041-2053) and tubular structure (Rothemund PW, Ekani-Nkodo A, Winfree E, et al.Design andcharacterization of programmable DNA nanotubes.J Am Chem Soc, 2004,126:16344-16352).2000, people such as LaBean constructed the self-assembly unit that the DNA chain is wound in three rows again, are called the TX module, each TX module all has 6 cohesive terminuss, can form more stable two-dimensional array (LaBean TH, Reif JH, Seeman NC, et al.Construction, analysis, ligation, and self-assembly of DNA triple crossover complexes.J Am ChemSoc, 2000,122:1848-1860).The common thinking of these DNA self-assemblies is to be spliced into big figure by the Watson-Click complementarity of little elementary cell by cohesive terminus, and based on such characteristics, they have also formed the common limitation, promptly are difficult to construct the irregular complex figure.2006, the thinking of a kind of brand-new DNA self-assembly that Rothemund proposes first, promptly DNA " paper folding art " has pulled through this limitation, has constructed the nano level shape and the pattern of various relative complex, has obtained the important breakthrough in DNA self-assembly field.At present, existing report by the folding multiple two-dimensional nano shape of DNA " paper folding art ", as square, rectangle, pentagram, smiling face etc. (Rothemund PW.Folding DNA to create nanoscaleshapes and patterns.Nature, 2006,440:297-302).But, these nanometer shapes all are symmetric figures, and, these nanometer shapes do not change into other materials such as metal after being self-assembled into by DNA, from solution, be not transferred in the air simultaneously yet, thereby limited complicated nanometer shape greatly in purposes such as aspects such as electron device manufacturings.
Through domestic and foreign literature retrieval to prior art, so far do not see the structure research report of complicated asymmetric nanometer shape is more arranged, and the complicated nanometer shape of the DNA in the solution is not transferred to airborne research report, not with the complicated nanometer shape metallization of DNA and be applied to the report of electron device manufacture view yet.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of method with dna molecular complex structure nanometer shape is provided, can use dna molecular complex structure two-dimensional symmetric and asymmetric nanostructure, and can and be transferred in the air, thereby realize the purposes of this method at aspects such as electron device manufacturings with the complicated nanometer shape metallization of the DNA in the solution.
For achieving the above object, the present invention is according to needed target shape (as complicated nanometer shapes such as electron devices) in the practical application, select a dna single chain as the scaffolding chain, fill up the profile of target shape by folding this scaffolding chain in the horizontal direction, and the programming dna single chain that obtains some weak points is that the sequence of staple chain makes them fix the trend of scaffolding chain according to the principle of base complementrity, and the self-assembly in solution obtains needed target shape by scaffolding ssdna molecule and staple ssdna molecule then.The present invention also further with the shape of electron device as target shape, with the DNA nanostructured metalization of the electron device shape in the solution that obtains and be transferred in the air, obtain the electron device of nanoscale.
The present invention includes following steps:
(1) select a dna single chain as the scaffolding chain, at needed target shape, with folding fill up this target shape profile of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.
(2) according to the sequence and the resulting broken line graph of scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, and wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks; The staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart.
(3) according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, self-assembly by between the two is folded into needed target shape with dna molecular, and this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid; Perhaps, DNA nanometer shape is used the atomic force microscope imaging in air again with the solution displacement damping fluid that contains magnesium ion and after drying up.
In the resulting solution that obtains of the present invention or airborne DNA nanometer shape have actual application value, can be used to make the electron device of nanoscale.At this moment, described target shape is the shape of electron device, if with in the solution that obtains or airborne DNA nanometer shape handle with metal-nitrate solutions, and metal ion is reduced to 0 valency with reductive agent, dry up with pure water cleaning, nitrogen then, can obtain the electron device of nanoscale.
Utilize method provided by the present invention to construct the complicated nanostructure of DNA, have the characteristic that operation is simple and feasible, and can construct the DNA nanostructure of symmetry or asymmetrical high complexity.Simultaneously, the present invention is with the complicated nanostructured metalization of DNA and be transferred in the air, obtain the electron device of nanoscale, be expected to break through the industrialization technology minimum process characteristic line breadth of present unicircuit, in the vast potential for future development that has aspect the nano patterning processing of integrated electronic circuit industry.
Description of drawings
Fig. 1 is the broken line graph according to the contour design scaffolding chain of map of China.
Among Fig. 1, a is the map of China synoptic diagram, and b is the scaffolding chain broken line graph according to the contour design of map of China.
Fig. 2 is for fixing the trend of scaffolding chain with the staple chain.
Among Fig. 2, a is 8 kinds of staple chains, and what b was the staple chain between horizontal line is fixing, and what c was the staple chain between vertical slit is fixing.
Fig. 3 is the atomic force microscope image.
Among Fig. 3, a is a figure that meets original design, and b is the distinguishable formation figure of a group.
Fig. 4 is the scaffolding chain broken line graph according to the shapes of inductors design.
Embodiment
Below in conjunction with drawings and Examples technical scheme of the present invention is further described.Should be understood that following examples only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1
Construct the imitative map of China shape of nanoscale in the present embodiment with dna molecular.Basic mentality of designing is the shape that is folded into map of China with a long dna single chain (being called the scaffolding chain), uses the dna single chain (being called the staple chain) of some weak points to fix this shape by the complementation with the scaffolding chain then.
Step 1: according to the draw broken line graph of expression scaffolding chain of the profile of map of China.
Select M13mp18 DNA as the scaffolding chain, at the map of China shape, with the folding profile that fill up map of China shape of scaffolding chain by horizontal direction.As shown in Figure 1, is two zones of thing with the main part of map of China with the center in the Hainan Island vertically partition that is as the criterion, northeast part and northwest part also respectively vertically partition be two zones, broken line is a starting point with Hainan Island mid point southernmost, horizontal folding from south to north, finish the zone in west side, arrive the northwestward northernmost the time then folding by north orientation south, finish the folding of northeast in the same way, further finish the zone in main part east side, get back to Hainan Island mid point southernmost at last.The line segment that connects between Taiwan and the continent is not counted in horizontally, and promptly this two segment DNAs chain only plays the effect in traction Taiwan on composition.Thus, obtain the broken line graph of scaffolding chain.
On the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.A pitch of double-stranded DNA approximates 10.67 bases, and the length of each section double-stranded DNA must be half pitch integral multiple (for example: 16 bases of 10.67 * 0.5 * 3 ≈), could guarantee the torsion minimum of DNA chain like this in burst.The length of a pitch approximates 3.6 nanometers, width approximates 2 nanometers, distance between the staple chain that distance between the two horizontal segment DNA two strandss depends on and scaffolding chain complementary is short, the spacing of staple chain is set at 1.5 pitch, and the spacing of then horizontal dna double chain is about 1 nanometer.So when drawing broken line graph, the distance between the horizontal line section also will satisfy this ratio.Also to consider simultaneously select length be the strand M13mp18 DNA of more than 7,000 base as the scaffolding chain, corresponding base sum can not surpass but should be as far as possible near this number on the broken line graph.
Therefore, one group of data that broken line graph shown in Figure 1 can be expressed as, each number is represented on the broken line graph ratio between the length of each horizontal line section of origin-to-destination successively, also represent on the scaffolding chain from 5 ' hold the base number of the DNA that each section of 3 ' end is horizontal, and this group data input of next step program just: (16 16 66 32 32 112 112 107 107 112 112 208 208 230 230 256 256,267 267 272 112 112 112 80 80 48 48 32 48 16 16 16 48 48 64 64 256 8,080 64 64 32 32 48 48 32 32 16 32 16 48 48 80 80 64 64 64 64 32 32 16 11,264 64 80 80 96 96 112 112 112 112 96 96 128 16 16 128 48 48 66 16 16).These group data are to obtain on the basis of hand dipping map of China.In that their satisfy to be measured under the situation of ratio between gained line segment length as much as possible, data are adjusted into 16 integral multiple, be 6 or 11 perhaps divided by 16 remainder.When the spacing that can make the staple chain like this was 1.5 pitch, all DNA chains were in the torsion minimum of burst.
Step 2: programming obtains the sequence of staple chain.
Can imagine, after a long straight line is folded into the shape of imitative map of China as shown in Figure 1, just need in the ranks and between vertical slit fixing in level.This is to realize by the reactive force between the complementary base of DNA.According to the Watson-Crick principle, A on the DNA chain and T complementation, C and G complementation, therefore can be according to the sequence and the resulting broken line graph of scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, makes the staple chain fix the trend of scaffolding chain by the complementation between the base during programming.The staple chain can be divided into eight kinds of a-h, and shown in Fig. 2 (a), thick black line is represented the scaffolding chain, and thick blue line is represented staple chain (arrow direction be 5 ' → 3 '), and thin black line is represented the complementation between the base.These eight kinds of staple chains can be divided into two types again, and wherein four kinds of staple chains of a-d are used for fixing of level on the doubling line chart in the ranks, and shown in Fig. 2 (b), their repeated combination can be finished fixing between various horizontal line.The staple chain that wherein lacks certain type of some bases still be can be regarded as the type, a or the b class staple chain of 8 bases of 3 ' end for example can occur lacking at the upper limb of figure, the left and right edges of figure also can occur lacking polybase base more situation.The staple chain of four kinds of e-h is used for fixing between vertical slit on the doubling line chart in addition, and shown in Fig. 2 (c), their repeated combination can be finished fixing between vertical slit.Except some the staple chain that appears at the pattern edge place, the length of most staple chains is 32 bases.The spacing of all staple chains is 1.5 pitch (16 bases).
According to above mentality of designing, programme with PERL.The scaffolding chain-ordering of program input is the genomic dna sequence of Orbivirus M13mp18, and total length is 7249 bases.Finding out one of them stem length is the hairpin structure of 20 bases, and simulation BsrBI enzyme cuts the scaffolding chain DNA is become wire, does not consider near 73 bases the hairpin structure, and the dna sequence dna that only stays length and be 7176 bases is as the scaffolding sequence.And then this sequence obtains constituting 219 staple chain-orderings of map of China, with 8 length of residue scaffolding chain complementary DNA chain-ordering that is 23 bases, and with 2 length of scaffolding chain complementary that are connected Taiwan DNA chain-ordering that is 32 bases.
Step 3: according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, the self-assembly by between the two is folded into dna molecular imitates the map of China shape.
Cut strand M13mp18 DNA (NEB company) with the BsrBI enzyme.Earlier M13mp18 DNA and one section complementary sequence that comprises the BsrBI restriction enzyme site are mixed, and add damping fluid and hatched 15 minutes for 37 ℃, add the BsrBI enzyme then and cut 2 hours.Enzyme is cut product phenol chloroform extracting, alcohol precipitation.
The M13mp18DNA that obtains behind 229 short dna chains of synthetic (Invitrogen company) and the alcohol precipitation is mixed.Every staple chain concentration is 160nM, and M13mp18 DNA concentration is 1.6nM, and cumulative volume is 100 μ l.
Mixed solution is annealed to 4 ℃ from 94 ℃ of degree on the PCR instrument, reduces by 0.01 ℃ p.s..
The imitative map of China nanometer shape of the DNA that self-assembly is good can be used the atomic force microscope imaging in damping fluid.
The sample atomic force microscope imaging that self-assembly is good.The good sample drop of one 5 μ l self-assembly is added on the mica of new cleavage, mica is bonded at double faced adhesive tape be placed on the iron plate on atomic force microscope (NanoscopeIIIa Multimode AFM, the Veeco company) sample table.The liquid tank that needle point (NP-S, resonant frequency～9.4KHz, force constant～0.38N/m, Veeco company) will be housed places the sample top, injects 1 * TAE/Mg of 30 μ l in groove
2+Can begin imaging after the damping fluid.For making imaging clear and avoid sample to be subjected to the damage of needle point and sample room interaction force, should reduce to be applied to the power on the needle point as far as possible.For reducing the interference that bubble brings in the damping fluid in the imaging process, can be before injection earlier to the damping fluid processing of bleeding.
The atomic force microscope imaging results shows about 150 nanometers of strong point of resulting imitative map of China, and about 120 nanometers of the widest part highly are 2 nanometers, shown in Fig. 3 (a), meet original design.The ratio that we will meet intrinsic structure (as a and the b among Fig. 3 (b)) and all distinguishable structures (as the c among Fig. 3 (b)) is defined as the productive rate of self-assembly, weighs the success or not of a design synthetic roughly with it.In our design, productive rate is 59% (sample number is 125), and the major cause that influences productive rate has more serious deformation of self-assembly figure (as c among Fig. 3 (b)) and the accumulation (as the bright spot of d in Fig. 3 (b)) of staple chain on the self-assembly figure.Simultaneously, there are some figures accumulative situation (as b and the d among Fig. 3 (b)) to occur, other figure do not link to each other (as a and the c among Fig. 3 (b)) with other figures.
Perhaps, the imitative map of China nanometer shape of DNA is used the atomic force microscope imaging in air again with the solution displacement damping fluid that contains magnesium ion and after drying up.
After observing imitative map of China shape DNA nanostructure, stop imaging.In liquid tank, inject Mg (AC) repeatedly
2Solution displacement 1 * TAE/Mg
2+Damping fluid.Take out liquid tank, take off from sample table after the iron plate of sticking sheet mica with Mg (AC)
2Solution washes several times gently, places on the sample table again after drying up with rubber pipette bulb again.Change airborne needle point piece and carry out imaging.
Embodiment 2
Construct the shapes of inductors of nanoscale in the present embodiment with dna molecular, and the DNA nanostructure is silver metallized and be transferred in the air in solution, construct the silver-colored inductance of nanoscale.
Step 1: according to the draw broken line graph of expression scaffolding chain of the profile of inductance.
With with embodiment 1 in the method for step 1 all fours, select M13mp18 DNA as the scaffolding chain, at shapes of inductors, with the folding profile that fill up shapes of inductors of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain as shown in Figure 4, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.
Step 2: programming obtains the sequence of staple chain.
With with embodiment 1 in the method for step 2 all fours, sequence and resulting broken line graph according to the scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, and wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks; The staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart.
Step 3: according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, the self-assembly by between the two is folded into shapes of inductors with dna molecular.
With with embodiment 1 in the method for step 3 all fours, obtain the DNA nanostructure of shapes of inductors, this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid.
Step 4: the DNA nanostructure of the shapes of inductors in the solution is silver metallized.
With the DNA nanostructure of the shapes of inductors in the solution at ammoniacal liquor (concentration 10%-15%) and AgNO
3Handled 30 minutes in the mixing solutions of (concentration is 0.5mM to 1mM);
Adding in formaldehyde (concentration 10%-15%) solution and handled 5 minutes, is 0 valency with silver ion reduction;
The about 10 μ l of the solution that absorption obtains clean 3 times with pure water on the mica of new cleavage then, and nitrogen dries up, and promptly obtains the silver-colored inductance of nanoscale.
Embodiment 3
Construct the shapes of inductors of nanoscale in the present embodiment with dna molecular, and, construct the copper inductance of nanoscale DNA nanostructure copper metallization and being transferred in the air in solution.
Step 1: according to the draw broken line graph of expression scaffolding chain of the profile of inductance.
With with embodiment 1 in the method for step 1 all fours, select M13mp18 DNA as the scaffolding chain, at shapes of inductors, with the folding profile that fill up shapes of inductors of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain as shown in Figure 4, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.
Step 2: programming obtains the sequence of staple chain.
With with embodiment 1 in the method for step 2 all fours, sequence and resulting broken line graph according to the scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, and wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks; The staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart.
Step 3: according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, the self-assembly by between the two is folded into shapes of inductors with dna molecular.
With with embodiment 1 in the method for step 3 all fours, obtain the DNA nanostructure of shapes of inductors, this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid.
Step 4: with the DNA nanostructure copper metallization of the shapes of inductors in the solution.
Nanostructure and Cu (NO with the shapes of inductors in the solution
3)
2In the darkroom, handled 5 minutes after (concentration is 0.1M) aqueous solution;
In above-mentioned mixing solutions, add the ascorbic acid usp/bp solution of 0.1M, cupric ion is reduced to 0 valency;
The about 10 μ l solution of the solution that absorption obtains were handled 5 minutes on the mica of new cleavage, cleaned 3 times with pure water then, and nitrogen dries up again, promptly obtains the copper inductance of nanoscale.
Embodiment 4
Construct the shapes of inductors of nanoscale in the present embodiment with dna molecular, and the DNA nanostructure is silver metallized in air, construct the silver-colored inductance of nanoscale.
Step 1: according to the draw broken line graph of expression scaffolding chain of the profile of inductance.
With with embodiment 1 in the method for step 1 all fours, select M13mp18 DNA as the scaffolding chain, at shapes of inductors, with the folding profile that fill up shapes of inductors of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain as shown in Figure 4, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.
Step 2: programming obtains the sequence of staple chain.
With with embodiment 1 in the method for step 2 all fours, sequence and resulting broken line graph according to the scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, and wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks; The staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart.
Step 3: according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, the self-assembly by between the two is folded into shapes of inductors with dna molecular.
With with embodiment 1 in the method for step 3 all fours, obtain the DNA nanostructure of shapes of inductors, this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid, with the solution displacement damping fluid that contains magnesium ion and after drying up, DNA nanometer shape is used the atomic force microscope imaging in air again.
Step 4: the DNA nanostructure of airborne shapes of inductors is silver metallized.
Draw ammoniacal liquor (concentration 10%-15%) and AgNO
3The about 20 μ l of the mixing solutions of (concentration is 0.5mM to 1mM) drip the nanostructured surface in the DNA of shapes of inductors, handle 30 minutes;
Adding in the formaldehyde solution (concentration 10%-15%) and handled 5 minutes, is 0 valency with silver ion reduction;
Clean 3 times with pure water then, nitrogen dries up again, promptly obtains the silver-colored inductance of nanoscale.
Embodiment 5
Construct the shapes of inductors of nanoscale in the present embodiment with dna molecular, and, construct the copper inductance of nanoscale DNA nanostructure copper metallization in air.
Step 1: according to the draw broken line graph of expression scaffolding chain of the profile of inductance.
With with embodiment 1 in the method for step 1 all fours, select M13mp18 DNA as the scaffolding chain, at shapes of inductors, with the folding profile that fill up shapes of inductors of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain as shown in Figure 4, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding.
Step 2: programming obtains the sequence of staple chain.
With with embodiment 1 in the method for step 2 all fours, sequence and resulting broken line graph according to the scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, and wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks; The staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart.
Step 3: according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, the self-assembly by between the two is folded into shapes of inductors with dna molecular.
With with embodiment 1 in the method for step 3 all fours, obtain the DNA nanostructure of shapes of inductors, this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid, with the solution displacement damping fluid that contains magnesium ion and after drying up, DNA nanometer shape is used the atomic force microscope imaging in air again.
Step 4: with the DNA nanostructure copper metallization of airborne shapes of inductors.
Draw Cu (NO
3)
2The about 40 μ l of (concentration is 0.1M) aqueous solution drip the nanostructured surface in the DNA of shapes of inductors, handle 4-6 minute in the darkroom;
Add 40 μ l ascorbic acid usp/bp solution (concentration 0.1M), cupric ion is reduced to 0 valency;
After 5-8 minute, clean with pure water, nitrogen dries up, and promptly obtains the copper inductance of nanoscale.
Claims (2)
1, a kind of method with dna molecular complex structure nanometer shape is characterized in that may further comprise the steps:
(1) select a dna single chain as the scaffolding chain, at needed target shape, with folding fill up this target shape profile of scaffolding chain by horizontal direction, obtain the broken line graph of scaffolding chain, on the broken line graph length of each horizontal line section respectively with folding after each section scaffolding chain on base number corresponding;
(2) according to the sequence and the resulting broken line graph of scaffolding chain, the dna single chain that programming obtains some weak points is the sequence of staple chain, make the staple chain fix the trend of scaffolding chain by the complementation between the base during programming, described staple chain is divided into two types, wherein one type staple chain is used for fixing of level on the doubling line chart in the ranks, and the staple chain of another kind of type is used for fixing between vertical slit on the doubling line chart;
(3) according to the synthetic corresponding dna molecular of the sequence of staple chain, scaffolding ssdna molecule and staple ssdna molecule are mixed in solution and anneal, self-assembly by between the two is folded into needed target shape with dna molecular, and this DNA nanometer shape can be used the atomic force microscope imaging in damping fluid; Perhaps, DNA nanometer shape is used the atomic force microscope imaging in air again with the solution displacement damping fluid that contains magnesium ion and after drying up.
2, according to the method with dna molecular complex structure nanometer shape of claim 1, it is characterized in that described target shape is the shape of electron device, with in the solution that obtains or airborne DNA nanometer shape handle with metal-nitrate solutions, and metal ion is reduced to 0 valency with reductive agent, dry up with pure water cleaning, nitrogen then, obtain the electron device of nanoscale.
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| CN103014146A (en) * | 2010-10-12 | 2013-04-03 | 上海交通大学 | Controllable distribution method of gold nanoparticles on DNA origami chip |
| CN103014145A (en) * | 2010-10-12 | 2013-04-03 | 上海交通大学 | Controllable distribution method of gold nanoparticles on DNA origami chip |
| CN103014145B (en) * | 2010-10-12 | 2014-06-11 | 上海交通大学 | Controllable distribution method of gold nanoparticles on DNA origami chip |
| CN109536489A (en) * | 2017-09-21 | 2019-03-29 | 南京大学 | A kind of DNA molecular watt or its nucleic acid nano structure and its application |
| CN108796012A (en) * | 2018-06-15 | 2018-11-13 | 南京工程学院 | The method for building photoresponse DNA nanostructure |
| CN114045283A (en) * | 2021-11-02 | 2022-02-15 | 四川大学 | DNA nano structure, construction method and application |
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