CN107727629A - Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification - Google Patents
Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification Download PDFInfo
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- CN107727629A CN107727629A CN201610655345.XA CN201610655345A CN107727629A CN 107727629 A CN107727629 A CN 107727629A CN 201610655345 A CN201610655345 A CN 201610655345A CN 107727629 A CN107727629 A CN 107727629A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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Abstract
The present invention relates to a kind of Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification：It is cleaned and dried after solid core fibres are carried out into surface cleaning with absolute ethyl alcohol in ultrasonic cleaner；The solid core fibres for obtaining cleaning are placed in the hydrofluoric acid solution configured, cleans up and spontaneously dries after carrying out surface modification treatment；Handled using the aurosol of nano particle, surface-modified solid core fibres are placed wherein, at the uniform velocity lift fiber upwards；The fiber of acquisition is cleaned with deionized water, and spontaneously dried, that is, obtains the Raman-enhanced regenerative skill fiber on gold nano particle modification solid core fibres surface.The preparation method is based on electrochemical principle, and selected reaction reagent, concentration and the time can carry out more preferable modification to solid core fibres surface, while so that nano-particle and fiber combine more firm, it is possible to increase the suction-operated to probe molecule, strengthen Raman detection signal.Enhancing intensity and the stability and repeatability of fiber can be greatly improved.
Description
Technical field
The invention belongs to Raman reinforcing material surface modification technology field, and in particular to a kind of preparation method of gold nano particle modification solid core fibres surface Raman enhancement type biological detection fiber.
Background technology
In recent years, surface modification nano material preparation, it is scattered and it is modified etc. receive extensive attention, the species of the coating material of application is also more and more, and the synthetic technology of dressing agent has also obtained very big development.The nano-particle of specific coating material modification often has specific function, is conventional lube oil additive such as with the DDP nano-particles modified.Therefore, exploitation novel surface dressing agent is expected to assign nano composite material new function.
On the one hand the surface modification of nano-particle protects nano-particle, improve the dispersiveness and compatibility of nano-particle, is laid a solid foundation for the preparation of High performance nanometer composite material；On the other hand the surface-active of nano-particle is changed, such as forms absorption or cladding in particle surface, the probability that the surface of particle and environment contact reduces, the activity decrease of nano-particle.Chemical modification method makes the surface of particle be totally different from initial material, also changes the original activity of nano-particle.
The emphasis studied from now on is the application field for expanding coating material, novel surface dressing agent is developed, to increase substantially the physical and chemical performance of nano composite material.Research in terms of Efforts To Develop self-assembly, using novel surface dressing agent and packaging technology, specific, the two dimension and three-dimensional structure of long-range order are obtained, obtains the characteristics such as required optics and electromagnetism.Study the cladding sex modification of magnetic nano-particle, improve the compatibility between magnetic nano-particle and biological tissue, further carry out application study of the magnetic nano-particle as targeted drug, biology sensor and biochip etc., paved the way for the extensive use of novel nano-material.
The content of the invention
For overcome the deficiencies in the prior art, the invention provides a kind of Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification, the preparation method is based on electrochemical principle, and selected reaction reagent, concentration and the time can carry out best modification to solid core fibres surface, while so that nano-particle and fiber combine more firm, the suction-operated to probe molecule can be improved, strengthens the Raman detection signal of biological information.Enhancing intensity and the stability and repeatability of fiber can be greatly improved.
A kind of Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification, it is characterised in that comprise the following steps：
（1）It is cleaned and dried after solid core fibres are carried out into surface cleaning with absolute ethyl alcohol in ultrasonic cleaner；
（2）By step（1）The cleaning optical fiber of acquisition is placed in the hydrofluoric acid solution configured, carries out that surface is modified cleans up and spontaneously dry；
（3）Handled using the aurosol of nano particle, by step（2）In surface-modified optical fiber place wherein, at the uniform velocity lifting solid core fibres upwards；
（4）By step（3）The fiber of middle acquisition is cleaned with deionized water, and is spontaneously dried, that is, obtains the Raman-enhanced regenerative skill biological detection fiber of gold nano particle modification optical fiber surface.
Step（1）In cleaning process need to be completed in ultrasonic cleaner to cleaning 20 min under the conditions of 70 ~ 80 DEG C, cleaning rate is 100 ~ 120 r/min.
Step（2）Middle surface modification process needs the concentration of dilute hydrofluoric acid solution, and diluted concentration is 10 %, and the reaction time is 10 ~ 15 min.
Step（3）The aurosol processing time of middle nano particle should be controlled in 40 s, and gold nano grain thickness control is in 20 ~ 30 nm.Compared with the conventional method compared with：The beneficial effects of the invention are as follows：
The present invention uses electrochemical method, and method is simple, effectively prevent particle agglomeration problem；Used surface modified concentration and time are as optimal optimal conditions, it is possible to increase to the absorption property of probe molecule；Prepared Raman-enhanced regenerative skill biological detection fiber has good stability and repeatability.
Accompanying drawing, table explanation
Fig. 1 is that Raman-enhanced regenerative skill fiber prepared by case study on implementation 4 of the present invention is 10 to concentration-6The Raman enhanced spectrum of M methylene blue；
Fig. 2 is the scanning electron microscope (SEM) photograph of the Raman-enhanced regenerative skill fiber prepared in case study on implementation 4 of the present invention；
Fig. 3 is the formation schematic diagram that the present invention prepares Raman-enhanced regenerative skill fiber.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is implemented under premised on technical solution of the present invention, gives detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following embodiments.
Embodiment 1:
20 ml absolute ethyl alcohols are added in totally dry beaker, the solid core fibres cut are soaked into interior；20 min are cleaned under the conditions of 70 ~ 80 DEG C in ultrasonic cleaner, and cleaning rate is 100 ~ 120 r/min, and naturally dry after being cleaned repeatedly with alcohol and deionized water is cooled to after room temperature；The distilled water that 10 ml 40% hydrofluoric acid solution is dissolved in 30 ml is added in another plastic beaker totally dried, is dipped a little with totally dry writing brush, coated in the solid core fibres surface cut, cleans simultaneously naturally dry after 15 min repeatedly with deionized water；The solid core fibres of naturally dry are put into the glass tubule equipped with aurosol, at the uniform velocity lift solid core fibres upwards（Such as Fig. 3）, control time dried after taking-up with deionized water rinsing in 40 s；The Raman-enhanced regenerative skill biological detection fiber on the gold nano particle modification solid core fibres surface that thickness is 20 nm can be obtained.
Embodiment 2:
20 ml absolute ethyl alcohols are added in totally dry beaker, the solid core fibres cut are soaked into interior；20 min are cleaned under the conditions of 70 ~ 80 DEG C in ultrasonic cleaner, and cleaning rate is 100 ~ 120 r/min, and naturally dry after being cleaned repeatedly with alcohol and deionized water is cooled to after room temperature；The distilled water that 10 ml 40% hydrofluoric acid solution is dissolved in 70 ml is added in another plastic beaker totally dried, is dipped a little with totally dry writing brush, coated in the solid core fibres surface cut, cleans simultaneously naturally dry after 15 min repeatedly with deionized water；The solid core fibres of naturally dry are put into the glass tubule equipped with aurosol, at the uniform velocity lift solid core fibres upwards（Such as Fig. 3）, control time dried after taking-up with deionized water rinsing in 40 s；The Raman-enhanced regenerative skill biological detection fiber on the gold nano particle modification solid core fibres surface that thickness is 20 nm can be obtained.
Embodiment 3:
20 ml absolute ethyl alcohols are added in totally dry beaker, the solid core fibres cut are soaked into interior；20 min are cleaned under the conditions of 70 ~ 80 DEG C in ultrasonic cleaner, and cleaning rate is 100 ~ 120 r/min, and naturally dry after being cleaned repeatedly with alcohol and deionized water is cooled to after room temperature；The distilled water that 10 ml 40% hydrofluoric acid solution is dissolved in 30 ml is added in another plastic beaker totally dried, is dipped a little with totally dry writing brush, coated in the solid core fibres surface cut, cleans simultaneously naturally dry after 30 min repeatedly with deionized water；The solid core fibres of naturally dry are put into the glass tubule equipped with aurosol, at the uniform velocity lift solid core fibres upwards（Such as Fig. 3）, control time dried after taking-up with deionized water rinsing in 30 s；The Raman-enhanced regenerative skill biological detection fiber on the gold nano particle modification solid core fibres surface that thickness is 20 nm can be obtained.
Embodiment 4:
20 ml absolute ethyl alcohols are added in totally dry beaker, the solid core fibres cut are soaked into interior；20 min are cleaned under the conditions of 70 ~ 80 DEG C in ultrasonic cleaner, and cleaning rate is 100 ~ 120 r/min, and naturally dry after being cleaned repeatedly with alcohol and deionized water is cooled to after room temperature；The distilled water that 10 ml 40% hydrofluoric acid solution is dissolved in 30 ml is added in another plastic beaker totally dried, is dipped a little with totally dry writing brush, coated in the solid core fibres surface cut, cleans simultaneously naturally dry after 15 min repeatedly with deionized water；The solid core fibres of naturally dry are put into the glass tubule equipped with aurosol, at the uniform velocity lift solid core fibres upwards（Such as Fig. 3）, control time dried after taking-up with deionized water rinsing in 80 s；The Raman-enhanced regenerative skill biological detection fiber on the gold nano particle modification solid core fibres surface that thickness is 40 nm can be obtained.
Raman enhanced spectrum test is carried out to case study on implementation sample, accompanying drawing 1 is case study on implementation（4）The fiber of middle acquisition is 10 to concentration-6 The Raman enhanced spectrum of M methylene blue probe molecule.Accompanying drawing 2 is the scanning electron microscope (SEM) photograph of the Raman-enhanced regenerative skill fiber.
Claims (4)
1. a kind of Raman-enhanced regenerative skill biological detection fiber producing processes based on gold nano particle modification, it is characterised in that comprise the following steps：
（1）It is cleaned and dried after solid core fibres are carried out into surface cleaning with absolute ethyl alcohol in ultrasonic cleaner；
（2）By step（1）The cleaning optical fiber of acquisition is placed in the hydrofluoric acid solution configured, carries out that surface is modified cleans up and spontaneously dry；
（3）Handled using the aurosol of nano particle, by step（2）In surface-modified optical fiber place wherein, at the uniform velocity lifting solid core fibres upwards；
（4）By step（3）The fiber of middle acquisition is cleaned with deionized water, and is spontaneously dried, that is, obtains the Raman-enhanced regenerative skill biological detection fiber of gold nano particle modification optical fiber surface.
A kind of 2. preparation method of the Raman-enhanced regenerative skill biological detection fiber based on gold nano particle modification according to claim 1, it is characterised in that step（1）In cleaning process need to be completed in ultrasonic cleaner to cleaning 20 min under the conditions of 70 ~ 80 DEG C, cleaning rate is 100 ~ 120 r/min.
A kind of 3. preparation method of the Raman-enhanced regenerative skill biological detection fiber based on gold nano particle modification according to claim 1, it is characterised in that step（2）Middle surface modification process needs the concentration of dilute hydrofluoric acid solution, and diluted concentration is 10 %, and the reaction time is 10 ~ 15
min。
A kind of 4. preparation method of the Raman-enhanced regenerative skill biological detection fiber based on gold nano particle modification according to claim 1, it is characterised in that step（3）The aurosol processing time of middle nano particle should be controlled in 40 s, and gold nano grain thickness control is 20 ~ 30
nm。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108020538A (en) * | 2018-01-02 | 2018-05-11 | 天津大学 | Human body IgG Protein Detection SERS fibers based on gold nano Sol-Modified |
| CN110373708A (en) * | 2019-07-31 | 2019-10-25 | 东南大学 | Adjustable concentration realizes that the nanometer pinpoint of needle point cone angle control prepares platform and method |
-
2016
- 2016-08-11 CN CN201610655345.XA patent/CN107727629A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108020538A (en) * | 2018-01-02 | 2018-05-11 | 天津大学 | Human body IgG Protein Detection SERS fibers based on gold nano Sol-Modified |
| CN110373708A (en) * | 2019-07-31 | 2019-10-25 | 东南大学 | Adjustable concentration realizes that the nanometer pinpoint of needle point cone angle control prepares platform and method |
| CN110373708B (en) * | 2019-07-31 | 2021-04-30 | 东南大学 | Nano needle tip preparation platform and method for adjusting concentration to realize needle tip cone angle control |
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