CN100581377C - Apocytochrome porcine hemoglobin hydrolyzates and protoheme peptide preparing method - Google Patents
Apocytochrome porcine hemoglobin hydrolyzates and protoheme peptide preparing method Download PDFInfo
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- CN100581377C CN100581377C CN200710031826A CN200710031826A CN100581377C CN 100581377 C CN100581377 C CN 100581377C CN 200710031826 A CN200710031826 A CN 200710031826A CN 200710031826 A CN200710031826 A CN 200710031826A CN 100581377 C CN100581377 C CN 100581377C
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Abstract
The invention discloses a method for preparing doff heme pig blood hemoglobin hydrolysate and heme peptide, wherein the method takes porcine erythrocytes as raw materials, and the doff heme pig blood hemoglobin hydrolysate and the heme peptide are prepared through hemolysis, enzymatic hydrolysis of compound protease and isoelectric centrifugal separation. The reaction conditions of the invention is mild, the recovery rate of the protein of the doff heme hydrolysate is 80-85 percent, the recovery rate of the heme in the heme peptide is 90-93 percent, and the content of heme is 25-30 percent. The two products do not have bad flavor, and are capable of being added in foods.
Description
Technical field
The present invention relates to the preparation method of a kind of collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte and heme peptide, be specifically related to the pig erythrocyte is raw material, by haemolysis, control protease hydrolyzed, etc. the ionization heart separate preparation collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte and heme peptide, belong to technical field of food biotechnology.
Background technology
Owing to historical and traditional reason, China delivers for sale with the quantity of slaughter pig extremely huge every year: according to the data of State Statistics Bureau, the amount of delivering for sale of China pig in 2004 is 6.4 hundred million, and except 2,000,000 live hog outlets more, the pig that the overwhelming majority delivers for sale all kills domestic butchering.Collect 2.2 kilograms of calculating of pig blood with every pig, then just there are 1,400,000 tons of pig blood output in China in 2004.Porcine hemoglobin is a part important in the blood, accounts for 65% of porcine haemoglobin total amount.Be rich in ferroheme in the hemoglobin, the 100g porcine hemoglobin contains heme iron 40mg, as ferroheme not being separated with hemoglobin, easily causes the bad change of Food Oxidation in the food and directly hemoglobin is added to, and product colour deepens, and this has seriously restricted the development and use of pig blood resource.Removing at present has the fraction hemoglobin to be used for the lower product of production added value, as blood bean curd, blood sausage and feeding blood meal, but other most of hemoglobin all directly discharging is discarded, caused the serious wasting of resources and environmental pollution.The method of separating ferroheme and albumen from hemoglobin is to adopt organic solvent method mostly, and this method causes organic solvent residual, and albumen is difficult to recycle, and environmental pollution is bigger.Also had scholar (Houlier, Synowiecki, Ockerman) to adopt enzyme hydrolysis method to extract hemoglobin in the erythrocyte afterwards, the product behind the enzymolysis adopts active carbon adsorption collapse due to massive hemorrhage red pigment.The protein recovery that adopts this method erythrocyte only is 60-70%, and enzymolysis product has heavier bitter taste, and ferroheme is difficult to recycle, and this has influenced applying of this method.After have the scholar to adopt the CMC absorption method from the hemoglobin enzymolysis product, to extract ferroheme, though avoided the interpolation organic solvent, in leaching process, need to add a large amount of water (9 times), the energy consumption of Protein Recovery increases, practical application is restricted.
The formula of measuring protein recovery, the ferroheme rate of recovery and degree of hydrolysis is as follows:
Total protein content * 100% in protein content/pig blood erythrocyte in protein recovery=collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte
Ferroheme total amount * 100% in content of hemachrome in the ferroheme rate of recovery=heme peptide/pig blood erythrocyte
Total nitrogen content in ammonia nitrogen content/supernatant * 100% in degree of hydrolysis=supernatant
Summary of the invention
The objective of the invention is to overcome that the above-mentioned energy consumption that prior art exists is big, the collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte rate of recovery is low or the heme peptide rate of recovery is low, environmental pollution is big and enzymolysis product has heavier defectives such as bitter taste, the preparation method of a kind of collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte and heme peptide is provided, make the protein recovery of collapse due to massive hemorrhage red pigment hemoglobin enzymolysis thing reach 80%～85%, the ferroheme rate of recovery of heme peptide reaches 90%-93%, and content of hemachrome reaches 25%～30%.The present invention adopts green production process, the raw material availability height.
The preparation method of collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte of the present invention and heme peptide comprises the steps:
(1) porcine hemoglobin adds water to adjust its protein content is 12.4%～17.5%, regulator solution pH value to 7.1～7.8, the mixing protease that adds pancreatin and Protamex or papain is hydrolyzed cessation reaction when degree of hydrolysis reaches 25%～31%;
(2) hydrolysate that step (1) is obtained centrifugal 10min～20min under 3000g～4000g condition obtains supernatant and precipitation respectively;
(3) the centrifugal supernatant that obtains of step (2) is transferred pH to 3.5～5.2,30 ℃～50 ℃ water bath heat preservation 10～60min after, the centrifugal 10min～20min of 3000g～4000g obtains supernatant and precipitation respectively;
(4) step (2) and (3) centrifugal precipitation that obtains are merged, the distilled water washing with 0.2～0.5 times of weight at the centrifugal 10min～20min of 3000g～4000g, obtains supernatant and precipitation respectively;
(5) be 7.0 with step (3) and (4) centrifugal supernatant accent pH that obtains, concentrate that spray-drying obtains faint yellow powdery collapse due to massive hemorrhage red pigment hemoglobin enzymolysis thing.The protein recovery of porcine hemoglobin can reach 80%～85%, and product does not have bitter taste, and its Protein content is 90%～93%;
(6) the centrifugal precipitation that obtains of step (4) is placed cold storage 12h～24h under-5 ℃～-20 ℃ conditions, 25 ℃ of distilled water washings that add 0.5 times of weight after thawing, centrifugal 10min～20min under 3000g～4000g condition, abandoning supernatant, obtain precipitation, to be deposited in cold storage under the similarity condition, washing, centrifugal, repeat 2～4 times, will precipitate the dry heme peptide that gets.
In step (1), the pH value of solution value is adjusted to 7.1～7.8 with 2M NaOH.
In step (1), with 95 ℃～100 ℃ heating 5min～10min enzymolysis reaction.
In step (3), the pH value of solution value is adjusted to 3.5～5.2 with 2M HCl.
In step (5), supernatant pH value is adjusted to 7.0 with 2M NaOH.
In step (6), adopt freeze-drying will precipitate the dry black powder that is, this moment, content of hemachrome reached 25%～30%, peptide or Protein content 70%～75%.
Described porcine hemoglobin solution can adopt prior art to obtain, the present invention adopts following method to prepare: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% natrium citricum and mix anti-freezing, pass through 3000g～4000g, 5 ℃～10 ℃ centrifugal 10min～20min then, lower sediment is an erythrocyte.The water that erythrocyte adds 1.0～1.5 times of volumes is that the ultrasonic processing of 1000W～1500W 2min～5min obtains porcine hemoglobin solution through 20Mpa～60Mpa homogeneous or ultrasonic power again.
Described protease is the food-grade albumen enzyme, and the concrete ratio of its mixing protease (U/U) is as follows:
Pancreatin: Protamex=1～2: 9;
Pancreatin: papain=1～2: 8.
The present invention compared with prior art, have following advantage: the present invention carries out haemolysis by high-pressure homogeneous or ultrasonic processing to erythrocyte and handles, adopt compound protease that hemoglobin is carried out efficient controlled hydrolysis, employing isoelectric point precipitation realization protein digestion product efficiently separates with heme peptide, has prepared and has met collapse due to massive hemorrhage red pigment enzymolysis hemoglobin powder and the heme peptide that food industry applications requires.This method reaction condition gentleness, non-secondary pollution, protein recovery reaches as high as 85%, has improved 10%～15% than the best level of present bibliographical information.The ferroheme rate of recovery reaches about 93%, and content of hemachrome reaches 25%～30% in the heme peptide, and two kinds of products all do not have bad local flavor.
The specific embodiment
Embodiment 1
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% natrium citricum and mix anti-freezing, 3000g, 5 ℃ of centrifugal 20min obtain the lower sediment erythrocyte, after erythrocyte added the water of 1.5 times of volumes, 20MPa homogeneous haemolysis obtained porcine hemoglobin solution.
Second step: porcine hemoglobin solution is added water adjust its protein content to 12.4%, mix the back and with 2MNaOH the pH value of solution value is adjusted to 7.1, add pancreatin and Protamex and be hydrolyzed, ratio (U/U) is pancreatin: Protamex=1: 9.When degree of hydrolysis reaches 30%, 100 ℃ of heating 5min enzymolysis reaction.
The 3rd step:, obtain precipitation and supernatant respectively with hydrolysate centrifugal 20min under the 3000g condition.
The 4th step: the 3rd step was obtained supernatant transfer pH to 3.5, behind 30 ℃ of water-bath 30min,, obtain supernatant and precipitation respectively at the centrifugal 20min of 3000g.
The 5th step: the precipitation in the 3rd step and the 4th step is merged, add the distilled water washing of 0.2 times of weight,, obtain supernatant and precipitation respectively at the centrifugal 20min of 3000g.
The 6th step: the supernatant in the 4th step and the 5th step is merged, adjust pH to 7.0, concentrated, spray-drying obtains faint yellow collapse due to massive hemorrhage red pigment zymolyte.Its protein content is 91.5%, and protein recovery is 80.6%.
The 7th step: the 5th step gained precipitation is placed-5 ℃ of cold storage 12h, add the distilled water washing of 0.5 times of weight after thawing, centrifugal 20min under the 3000g condition, abandoning supernatant, obtain precipitation, should precipitate, get heme peptide by aforesaid operations reprocessing in this step 2 times.
The 8th step: adopting freeze-drying is black powder with the 7th step gained heme peptide drying, and the rate of recovery of ferroheme reaches 90.6%, and content of hemachrome is 26.8% in the heme peptide.
Embodiment 2
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% natrium citricum and mix anti-freezing, it is centrifugal 10 minutes to pass through 4000g, 10 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.4 times of volumes obtains porcine hemoglobin solution through 60Mpa homogeneous haemolysis again.
Second step: porcine hemoglobin solution is added water, and to adjust its protein content be 13.5%, mix the back and the pH value of solution value is adjusted to 7.3 with 2MNaOH, add pancreatin and Protamex, ratio is pancreatin: Protamex=2: 9, when degree of hydrolysis reaches 28%, 95 ℃ of heating 10min enzymolysis reaction.
The 3rd step: hydrolysate at the centrifugal 15min of 3500g, is obtained precipitation and supernatant respectively.
The 4th step: the supernatant in the 3rd step is transferred pH to 4.0, and behind 40 ℃ of water-bath 30min, centrifugal 15min under the 3500g condition obtains supernatant and precipitation respectively.
The 5th step: the precipitation in the 3rd step and the 4th step is merged, add the distilled water washing of 0.3 times of weight, centrifugal 10min under the 4000g condition obtains supernatant and precipitation respectively.
The 6th step: the supernatant in the 4th step and the 5th step is merged, and adjust pH is 7.0, concentrates, and spray-drying obtains faint yellow product.Its protein content is 90.2%, and protein recovery is 82.1%.
The 7th step: the 5th step gained precipitation is placed-10 ℃ of cold storage 12h, add the distilled water washing of 0.5 times of weight after thawing, centrifugal 20min under the 3000g condition, abandoning supernatant, obtain precipitation, should precipitate, get the heme peptide product by aforesaid operations reprocessing in this step 3 times.
The 8th step: the gained heme peptide freeze drying of the 7th step is got black powder, and content of hemachrome is 25.9%, and the rate of recovery of ferroheme reaches 91.2%.
Embodiment 3
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, adds 0.33% natrium citricum and mixes anti-freezing, and centrifugal 15 minutes of 3500g, 5 ℃ obtain the lower sediment erythrocyte.The water that erythrocyte adds 1.3 times of volumes is that the ultrasonic 3min haemolysis of 1100W obtains porcine hemoglobin solution through ultrasonic power.
Second step: porcine hemoglobin solution adds water, and to adjust its protein content be 16.5%, with 2M NaOH the pH value of solution value is adjusted to 7.4, add pancreatin and papain, ratio is a pancreatin: papain=2: 8 (U/U), when degree of hydrolysis reaches 26%, 100 ℃ of heating 10min enzymolysis reaction.
The 3rd step: enzymolysis product at the centrifugal 10min of 4000g, is obtained precipitation and supernatant respectively.
The 4th step: the supernatant in the 3rd step is transferred pH to 4.5, behind 50 ℃ of water-bath 10min,, obtain supernatant and precipitation respectively at the centrifugal 15min of 3500g.
The 5th step: the precipitation in the 3rd step and the 4th step is merged, add the distilled water washing of 0.5 times of weight, centrifugal 10min under the 4000g condition obtains supernatant and precipitation respectively.
The 6th step: the supernatant in the 4th step and the 5th step is merged, and adjust pH is 7.0, concentrates, and spray-drying obtains faint yellow product.Its protein content is 90.5%, and protein recovery is 84.3%.
The 7th step: the 5th step gained precipitation is placed-20 ℃ of cold storage 12h, add the distilled water washing of 0.5 times of weight after thawing, centrifugal 15min under the 3500g condition, abandoning supernatant obtains precipitation.Should precipitate by aforesaid operations reprocessing in this step 4 times, get the hemoglobin peptide.
The 8th step: with the gained hemoglobin peptide freeze drying of the 7th step is black powder.The content of hemachrome of black powder is 26.4%, and the rate of recovery of ferroheme reaches 92.7%.
Embodiment 4
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, adds 0.33% natrium citricum and mixes anti-freezing, and centrifugal 15 minutes of 3500g, 5 ℃ obtain the lower sediment erythrocyte.The water that erythrocyte adds 1.1 times of volumes is that the ultrasonic 2min haemolysis of 1500W obtains porcine hemoglobin solution through ultrasonic power.
Second step: porcine hemoglobin solution adds water, and to adjust its protein content be 17.5%, mix the back and the pH value of solution value is adjusted to 7.7 with 2MNaOH, add pancreatin and papain, ratio is a pancreatin: papain=1: 8 (U/U), when degree of hydrolysis is 25%, 100 ℃ of heating 8min enzymolysis reaction.
The 3rd step: enzymolysis product at the centrifugal 20min of 4000g, is obtained precipitation and supernatant respectively.
The 4th step: the supernatant in the 3rd step is transferred pH to 5.0, and behind 40 ℃ of water-bath 40min, the centrifugal 10min of 4000g obtains supernatant and precipitation respectively.
The 5th step: the precipitation in the 3rd step and the 4th step is merged, add the distilled water washing of 0.2 times of weight,, obtain supernatant and precipitation respectively at the centrifugal 15min of 4000g.
The 6th step: the supernatant in the 4th step and the 5th step is merged, and adjust pH is 7.0, concentrates, and spray-drying obtains faint yellow product.The protein content of faint yellow product is 91.4%, and protein recovery is 84.1%.
The 7th step: the 5th step gained precipitation is placed-20 ℃ of cold storage 24h, add the distilled water washing of 0.5 times of weight after thawing, at the centrifugal 18min of 3000g, abandoning supernatant, obtain precipitation, should precipitate, get the hemoglobin peptide by aforesaid operations reprocessing in this step 3 times.
The 8th step: with the gained hemoglobin peptide freeze drying of the 7th step is black powder.Content of hemachrome is 29.9% in the black powder, and the rate of recovery of ferroheme reaches 90.4%.
Claims (6)
1, the preparation method of a kind of collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte and heme peptide is characterized in that comprising the steps:
(1) porcine hemoglobin solution adds water to adjust its protein content is 12.4%～17.5%, regulator solution pH value to 7.1～7.8, the mixing protease that adds pancreatin and Protamex or papain carries out enzymolysis, enzymolysis reaction when degree of hydrolysis reaches 25%～31%, the ratio of described mixing protease (U/U) are pancreatin: Protamex=1～2: 9 or pancreatin: papain=1～2: 8;
(2) enzymolysis product that step (1) is obtained centrifugal 10min～20min under 3000g～4000g condition obtains supernatant and precipitation respectively;
(3) the centrifugal supernatant that obtains of step (2) is transferred pH to 3.5～5.2,30 ℃～50 ℃ water-bath 10min～60min after, centrifugal 10min～20min under 3000g～4000g condition obtains supernatant and precipitation respectively;
(4) step (2) and (3) centrifugal precipitation that obtains are merged, the distilled water washing with 0.2～0.5 times of weight at the centrifugal 10min～20min of 3000g～4000g, obtains supernatant and precipitation respectively;
(5) step (3) and (4) centrifugal supernatant that obtains are merged, transfer pH to 7.0, concentrate, spray-drying obtains faint yellow powdery collapse due to massive hemorrhage red pigment porcine hemoglobin zymolyte;
(6) the centrifugal precipitation that obtains of step (4) is placed cold storage 12h～24h under-5 ℃～-20 ℃ conditions, add the distilled water washing of 0.5 times of weight after thawing, centrifugal 10min～20min under 3000g～4000g condition, abandoning supernatant, obtain precipitation, should precipitate set by step (6) middle aforesaid operations reprocessing 2～4 times, freeze drying gets heme peptide.
2, method according to claim 1 is characterized in that in the step (1), with 2M NaOH regulator solution pH to 7.1～7.8.
3, method according to claim 2 is characterized in that in the step (1), enzymolysis reaction after heating 5min～10min under 95 ℃～100 ℃ conditions.
4, method according to claim 3 is characterized in that step (3), with 2M HCl the pH value of solution value is adjusted to 3.5～5.2.
5, method according to claim 4 is characterized in that step (5), with 2M NaOH supernatant pH value is adjusted to 7.0.
6, method according to claim 5, it is characterized in that described porcine hemoglobin adopts following method to prepare: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% natrium citricum and mix anti-freezing, through 3000g～4000g, 5 ℃～10 ℃ centrifugal 10min～20min, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.0～1.5 times of volumes is the ultrasonic processing of 1000W～1500W 2min～3min through 20Mpa～60Mpa homogeneous or ultrasonic power again, and haemolysis obtains porcine hemoglobin solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791039A (en) * | 2010-03-23 | 2010-08-04 | 湖南农业大学 | Method for preparing high-F value pig blood oligopeptide |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101353686B (en) * | 2008-09-09 | 2011-09-21 | 浙江大学 | Production method of haemachrome and decolorizing blood plasma albumen powder |
| CN101375702B (en) * | 2008-09-12 | 2011-05-18 | 中国农业大学 | Hemoglobin polypeptide powder and preparation method thereof |
| CN101766251A (en) * | 2008-12-30 | 2010-07-07 | 天津市食品工业生产力促进中心 | Method for extracting modified plasma protein powder and bioactive peptide for enriching blood from pig blood |
| CN102187937A (en) * | 2011-03-22 | 2011-09-21 | 湖北宝迪农业科技有限公司 | Method for producing globulin zymolytes of porcine blood |
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| CN104256199A (en) * | 2014-10-16 | 2015-01-07 | 浙江索纳克生物科技有限公司 | Loach feed added with porcine hemoglobin powder |
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-
2007
- 2007-11-30 CN CN200710031826A patent/CN100581377C/en not_active Expired - Fee Related
Non-Patent Citations (10)
| Title |
|---|
| 富血红素多肽生产初探. 李华等.食品工业科技，第2期. 2007 |
| 富血红素多肽生产初探. 李华等.食品工业科技，第2期. 2007 * |
| 生化工艺学. 陈来同编著，25-27,258-267,440-447，科学出版社. 2004 |
| 生化工艺学. 陈来同编著，25-27,258-267,440-447，科学出版社. 2004 * |
| 脱色猪血红蛋白酶解产物品质及营养特性研究. 郭善广等.食品与发酵工业，第33卷第1(Total 229)期. 2007 |
| 脱色猪血红蛋白酶解产物品质及营养特性研究. 郭善广等.食品与发酵工业，第33卷第1(Total 229)期. 2007 * |
| 血红素的应用与生产技术研究进展. 李志钊等.食品研究与开发，第21卷第5期. 2000 |
| 血红素的应用与生产技术研究进展. 李志钊等.食品研究与开发，第21卷第5期. 2000 * |
| 诸血红蛋白酶解液脱色技术研究. 于美娟等.肉类工业，第8(总第304期)期. 2006 |
| 诸血红蛋白酶解液脱色技术研究. 于美娟等.肉类工业，第8(总第304期)期. 2006 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791039A (en) * | 2010-03-23 | 2010-08-04 | 湖南农业大学 | Method for preparing high-F value pig blood oligopeptide |
| CN101791039B (en) * | 2010-03-23 | 2011-04-20 | 湖南农业大学 | Method for preparing high-F value pig blood oligopeptide |
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