CN100558411C - 腱生蛋白-c核酸配体 - Google Patents

腱生蛋白-c核酸配体 Download PDF

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CN100558411C
CN100558411C CNB2004100629077A CN200410062907A CN100558411C CN 100558411 C CN100558411 C CN 100558411C CN B2004100629077 A CNB2004100629077 A CN B2004100629077A CN 200410062907 A CN200410062907 A CN 200410062907A CN 100558411 C CN100558411 C CN 100558411C
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nucleic acid
tenascin
rna
selex
nucleic acids
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CN1589908A (zh
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布赖恩·希克
史蒂芬·沃伦
戴维·帕马
拉里·戈尔德
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Gilead Sciences Inc
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Gilead Sciences Inc
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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6232071B1 (en) * 1990-06-11 2001-05-15 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US7005260B1 (en) * 2000-01-28 2006-02-28 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US7645743B2 (en) * 1999-12-22 2010-01-12 Altermune, Llc Chemically programmable immunity
CA2328356A1 (en) 1999-12-22 2001-06-22 Itty Atcravi Recreational vehicles
US7300922B2 (en) 2001-05-25 2007-11-27 Duke University Modulators of pharmacological agents
US20040249130A1 (en) * 2002-06-18 2004-12-09 Martin Stanton Aptamer-toxin molecules and methods for using same
JP2005533794A (ja) * 2002-06-18 2005-11-10 アーケミックス コーポレイション アプタマー−毒素分子およびこれを使用する方法
US8853376B2 (en) 2002-11-21 2014-10-07 Archemix Llc Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics
US8039443B2 (en) * 2002-11-21 2011-10-18 Archemix Corporation Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics
US10100316B2 (en) 2002-11-21 2018-10-16 Archemix Llc Aptamers comprising CPG motifs
US7727969B2 (en) * 2003-06-06 2010-06-01 Massachusetts Institute Of Technology Controlled release nanoparticle having bound oligonucleotide for targeted delivery
US20080268478A1 (en) * 2005-03-01 2008-10-30 Cedars-Sinai Medical Center Use of Eotaxin as a Diagnostic Indicator For Atherosclerosis and Vascular Inflammation
AR052741A1 (es) * 2005-04-08 2007-03-28 Noxxon Pharma Ag Acidos nucleicos de union a ghrelin
JP5603074B2 (ja) 2006-09-08 2014-10-08 ピラマル イメージング ソシエテ アノニム 18f標識物質のための化合物と方法
EP1897562A1 (en) * 2006-09-08 2008-03-12 Bayer Schering Pharma Aktiengesellschaft Aptamers labelled with Gallium-68
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
DK2172566T4 (da) * 2007-07-17 2022-06-13 Somalogic Inc Fremgangsmåde til generering af aptamerer med forbedrede off-rates
EP2036981A1 (en) * 2007-09-12 2009-03-18 Bayer Schering Pharma Aktiengesellschaft Aptamers labeled with 18F
BRPI1011269A2 (pt) 2009-05-05 2016-09-27 Altermune Technologies Llc imunidade quimicamente programável
US8841429B2 (en) 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
GB201114662D0 (en) 2011-08-24 2011-10-12 Altermune Technologies Llc Chemically programmable immunity
WO2013088140A2 (en) * 2011-12-12 2013-06-20 Isis Innovation Limited Biomarker and use thereof
CN102533772B (zh) * 2011-12-12 2013-10-09 中国科学院武汉病毒研究所 一种抗hbv诱饵适体及其筛选方法
JP6453212B2 (ja) 2012-07-13 2019-01-16 ウェイブ ライフ サイエンシズ リミテッドWave Life Sciences Ltd. キラル制御
CN107058596A (zh) * 2017-06-19 2017-08-18 上海市第十人民医院 一种与恶性胶质瘤诊断相关的标志物及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2183661B (en) 1985-03-30 1989-06-28 Marc Ballivet Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5789157A (en) * 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5496938A (en) * 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US6610841B1 (en) * 1997-12-18 2003-08-26 Gilead Sciences, Inc. Nucleotide-based prodrugs
US6127119A (en) * 1990-06-11 2000-10-03 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
US6232071B1 (en) * 1990-06-11 2001-05-15 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
EP0786469B1 (en) * 1990-06-11 2006-03-01 Gilead Sciences, Inc. Methods of use of nucleic acid ligands
CA2104698A1 (en) 1991-02-21 1992-08-22 John J. Toole Aptamers specific for biomolecules and methods of making
US5683874A (en) * 1991-03-27 1997-11-04 Research Corporation Technologies, Inc. Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence
US5582981A (en) * 1991-08-14 1996-12-10 Gilead Sciences, Inc. Method for identifying an oligonucleotide aptamer specific for a target
US5436132A (en) * 1993-02-13 1995-07-25 Amano Pharmaceutical Co., Ltd. Quantitative determination of tenascin as glioma marker
US5859228A (en) * 1995-05-04 1999-01-12 Nexstar Pharmaceuticals, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6229002B1 (en) * 1995-06-07 2001-05-08 Nexstar Pharmaceuticlas, Inc. Platelet derived growth factor (PDGF) nucleic acid ligand complexes
DE69735292T2 (de) * 1996-10-25 2006-10-19 Gilead Sciences, Inc., Foster City Gefaesszellen wachstumsfaktor (vegf) nukleinsäureligand-komplexe
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ALL YOU WANTED TO KNOW ABOUT SELEX. kKLUG S J ET AL.MOLECULAR BIOLOGY REPORT, KLUWER ACADEMIC PUBLISHERS, DORDRECHT,Vol.20 No.2. 1994
ALL YOU WANTED TO KNOW ABOUT SELEX. kKLUG S J ET AL.MOLECULAR BIOLOGY REPORT, KLUWER ACADEMIC PUBLISHERS, DORDRECHT,Vol.20 No.2. 1994 *
Biodistribution of 111IN-labelled SCN-bz-dtpa-sc-2 MAbfollwoing loco-regional injection info gliblastomas. MERLO A ET AL.International Journal of Cancer,Vol.71 . 1997
Biodistribution of 111IN-labelled SCN-bz-dtpa-sc-2 MAbfollwoing loco-regional injection info gliblastomas. MERLO A ET AL.International Journal of Cancer,Vol.71. 1997 *
Inhibition of human angiogenin by DNA aptamers, Nuclearcolocalization of an angiogenin-inhibitor complex. NOBILE VALENTINA et al.BIOCHEMISTRY , AMERICAN CHEMICAL SOCIETY EASTON,Vol.37 No.19. 1998
Inhibition of human angiogenin by DNA aptamers, Nuclearcolocalization of an angiogenin-inhibitor complex. NOBILE VALENTINA et al.BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY EASTON,Vol.37 No.19. 1998 *
Progress towards therapeutic application of Selex-DERIVEDAPTAMERS. POLISKY B.THE MANY FACES OF RNA. 1997

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