CN100553495C - Disperse the method for phytosterol and the beverage that contains nano level phytosterol - Google Patents

Disperse the method for phytosterol and the beverage that contains nano level phytosterol Download PDF

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Publication number
CN100553495C
CN100553495C CNB018165923A CN01816592A CN100553495C CN 100553495 C CN100553495 C CN 100553495C CN B018165923 A CNB018165923 A CN B018165923A CN 01816592 A CN01816592 A CN 01816592A CN 100553495 C CN100553495 C CN 100553495C
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phytosterol
beverage
water
emulsifying agent
mixture
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CN1468066A (en
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尹源泰
金甲植
金甫泉
韩政希
洪亨杓
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Eugene Science Inc
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Eugene Science Inc
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A23L33/11—Plant sterols or derivatives thereof, e.g. phytosterols
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y99/00—Subject matter not provided for in other groups of this subclass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/24—Heat, thermal treatment
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00—Processes
    • A23V2300/26—Homogenisation

Abstract

The invention discloses the method for the phytosterol that disperses to be used for beverage and the beverage that phytosterol disperses, the granular size of phytosterol is a nanoscale in dispersed beverage.The dispersion of phytosterol is at first with phytosterol and at least a emulsifier that is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester, then by making mixture melt 60-200 ℃ of heating.Then, with the material and the water-based beverage of fusing, or under the state of himself fusing type or powder-type, mix with the water-based beverage that contains emulsifying agent.Thereby the mixture high-speed stirred of this gained is generated the dispersion of phytosterol in water-based beverage.This beverage is excellent aspect bioavailability, has good mouthfeel, transparent appearance, and peculiar taste, fragrance and the color of beverage do not influenced.

Description

Disperse the method for phytosterol and the beverage that contains nano level phytosterol
Technical field
The present invention relates to phytosterol is disperseed to be used for the method for beverage and the beverage that phytosterol disperses.More specifically, the present invention relates to prepare the method for phytosterol dispersion, wherein the phytosterol micelle size of Xing Chenging is hundreds of nanometers or littler, and relates to the beverage that contains this dispersion.
Background technology
Recently, the excessive more and more absorption cholesterol of people, its result causes the disease relevant with cholesterol to become big social concern just day by day.Especially Asians, their life style imitation westization comprises diet, owing to heat is loyal to rapid-result food and fast food, like this they consume high cholesterol food chance than more in the past.In case take in, the cholesterol in these food has just increased the cholesterol level in the blood, and may become angiocardiopathy, comprises the main inducing of hyperlipidemia, artery sclerosis, cardiac arrhythmia, heart infarct etc.
Metabolic process by the research cholesterol has shown that the cholesterol in Nei Sheng and the diet all enters small intestine, and about 50% cholesterol is by intestinal absorption (Bosner, M.S., Ostlund, R.E., Jr., Osofisan, O., Grosklos, J., Fritschle, C., Lange, L.G.1993).Based on this fact, stop cholesterol by the mechanism of action of intestinal absorption for those be devoted to seek clue is used to prevent and the people of treatment and cholesterol related diseases have special significance.
Phytosterol or phytosterol can be divided into sitosterol, campesterol and stigmasterol, and phytosterol or phytosterol comprise sitosterol and campesterol, for simplicity, they are referred to as phytosterol here.
Because the structure of phytosterol and the structure fairly similar of cholesterol, the known plants sterol suppresses the intestinal absorption cholesterol, thereby reduces the content of serum cholesterol, as U.S. Patent number 5,578, and 334 announcements.As naturally occurring material, phytosterol is nontoxic, and can find in vast plant spectrum, as soybean, corn, trees, Tall oil (tall oil) etc.Utilize phytosterol to suppress the function of intestinal absorption cholesterol, clinical testing is used for treating angiocardiopathy, coronary artery disease and hyperlipidemia (Atherosclerosis28:325-338) with phytosterol as therapeutic agent.
Although this useful function is arranged, phytosterol is difficult to food, this be since its physical characteristic promptly the dissolubility in water and oil is too poor causes.Thereby general public can only absorb phytosterol limitedly.
In order to increase the solubility of phytosterol, some research workers have synthesized the derivative of each plant sterols.For example, developed the ester type compound of phytosterol, they have fabulous solubility (Mattson F.H., R.A.Volpenhein and B.A.Erickson, 1997) in oil phase.U.S. Patent number 5,502,045 discloses sitosterol fatty ester, and it carries out esterification by sitosterol and aliphatic acid and prepares.When patent report in view of the above, this sitosterol fatty ester use with useful form in oil phase (margarine), the content that can reduce LVL-C nearly 16%.
Water-soluble and the oil-soluble phytosterol derivative that WO 99/15546 and WO 99/15547 describes is connected water or oil-soluble molecule by ester bond and synthesizes with phytosterol or plant stanol.
But some results of study show, the synthesizing phytosterol derivative that dissolubility improves is to the strong (Mattson etc. of the too late natural phytosterin of the inhibitory action of intestinal absorption cholesterol, TheAmerican Journal of Clinical Nutrition 35:April 1982, the 697-700 pages or leaves).Particularly the shortcoming of this oil-soluble derivative is also to absorb many edible oils simultaneously.
Except the derivative by synthesizing phytosterol improves the dissolubility of phytosterol with effort, carried out and continued the bioavailability that further investigation improves phytosterol.
A large amount of work has been done in the research of relevant this respect.For example, develop oral medicinal sitosterol and can disperse powder, it is that sitosterol, glucidtemns, silica and polyoxygenated dimethylbenzene (polyoxylene) sorbitan monostearate are mixed by a certain percentage, make by homogenate, the degassing, pasteurization and evaporation, as U.S. Patent number 3,881,005 announcements.
U.S. Patent number 5,932,562 disclose the water homogeneous phase micelle mixture of a plant sterols, lecithin and lysolecithin, and it is dried to the water-soluble powder of segmentation.Its preparation method is that phytosterol, lecithin and lysolecithin are mixed in chloroform together with fixing mol ratio, removes chloroform then.But this patent has also been brought some problems.Wherein the total amount of used emulsifying agent is far longer than the consumption of phytosterol.The emulsifying agent lysolecithin is very expensive.The organic solvent that is used for forming micelle worse makes that water-soluble powder is unsuitable for absorbing.
Other water-soluble plant sterols also can be at U.S. Patent number 6,054, finds in 144 and 6,110,502.According to these patents, the production method of water dispersible phytosterol is that rice sitosterol or phytosterol, simple function group surfactant and polyfunctional group surfactant are mixed in water by a certain percentage, then drying composite.The feature of this production method is to remove homogenate and degassing step from, and adopts polyoxygenated dimethylbenzene sorbitan monopalmitate and sorbitan monopalmitate respectively as simple function group surfactant and polyfunctional group surfactant.
European patent publication numbers 289,636 have described the method for the sterol of a kind of production stable type emulsification or solubilising, it is that phytosterol is mixed by a certain percentage with the polyol liquid that contains sucrose fatty ester and/or polyglyceryl fatty acid ester, again this mixture of dilute with water.When this was used for beverage, its size of the micelle particle of the phytosterol that is produced was big to tens of microns, and its mouthfeel is as the bristle.In addition, the shortcoming of this micelle particle is that beverage is opaque.
The food ingredients that can be used for reducing cholesterol level are at U.S. Patent number 6,190, and are open in 720.This patent has also been introduced the method for preparing food ingredients, and it is that the phytosterol of one or more fusings is become homogeney with one or more fat with one or more emulsifier, under agitation cools off this homogenate mixture to about 60 ℃ and must pastel.This food ingredients can be used for oil base (oil-based) food such as salad flavor enhancement, margarine etc.As expected, this food ingredients can not be used for water-based beverage in fact, and this is because its dispersion stabilization can only obtain in fat.
European patent 0897671A1 relates to the aqueous dispersion of the phytosterol that is used for tablespread, flavouring, milk, cheese etc., and relate to the preparation method, it comprises that the emulsifying agent of the high-melting-point lipid that will melt, non-sterol and water are mixed together, and is characterized in that dystectic lipid mean size is 15 microns or littler under shearing.Water-borne dispersions has makes saturated fat and trans-fatty acid is minimum or the advantage eliminated.But, must carry out the micronizing of high-melting-point lipid such as phytosterol.In addition, because its dispersion stabilization is low in water, this dispersion can not be used for water-based beverage.
The edible product that reduces cholesterol can find in WO 00/33669.According to the method for this patent, phytosterol is dissolved in or is blended in the melt of food emulsifying agent, mix with protein-contg food such as milk or sour milk, homogenate adds in the food then.The edible product of this reduction cholesterol is only containing the stability that could keep its dispersion in the presence of the protein substance, does not contain protein substance and just can not keep its stability if do not exist.So the beverage that the edible product of this reduction cholesterol is used for not containing protein substance is very difficult.
U.S. Patent number 6,267,963 relate to the compound of one plant sterols-emulsifying agent, and its fusing point hangs down 30 ℃ at least than the fusing point of phytosterol, it is characterized in that, because its fusing point reduces, in food processing process or afterwards, the unlikely crystallization of phytosterol-emulsifying agent, and can effective dose be incorporated in the food to reduce the content of serum cholesterol, thereby people are when eating these products, need not to modify on the contrary the structure of food.Compound by phytosterol, emulsifying agent, neutral lipid such as triglycerides preparation can be used in the oil base food.If there is not neutral lipid, can be with hard ester acyl lactylate as emulsifying agent.But be to use hard ester acyl lactylate to be subjected to the restriction of law.In addition, when this compound was used for beverage, its peculiar niff also needed to cover technology.
Disclosure of the Invention
Consider the problem of above existence, after the soluble form to phytosterol gos deep into and comprehensively studies, the inventor finds when not having other compositions, phytosterol is heated with emulsifying agent, make their even contact each other, fusion, big slight under high-speed stirred and homogenate effect, to form to nano level tiny micelle, cause the present invention thus.Also find in beverage, nano level micelle is excellent aspect bioavailability, peculiar taste and not influence of fragrance to beverage, except being used for nearly all beverage, do not consider the matrix and the pH value of beverage, and the life cycle that improves the stably dispersing performance prolongation beverage of phytosterol micelle, guarantee that product has long-term stability.
Therefore, the purpose of this invention is to provide a kind of method that makes things convenient for form that in aqueous matrix, phytosterol is dispersed into, described form is except to the peculiar taste of application beverage and fragrance not the influence, also is applicable to beverage and improves the bioavailability of phytosterol.
Another object of the present invention provides a kind of beverage that contains the phytosterol dispersion, and this beverage mouthfeel is not bristly.
Another object of the present invention provides a kind of additive that is applicable to beverage, and this additive can prepare with the inventive method.
According to one embodiment of the invention, a kind of method of disperseing phytosterol is provided, it comprises the steps: under 60-200 ℃ of temperature, with the mixture heat fusing of phytosterol and at least a emulsifying agent, described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester; Mixture and the water-based beverage of fusing or the water-based beverage that contains emulsifying agent are mixed; The high-speed stirred mixture is to obtain the beverage of phytosterol dispersion, and phytosterol can be dispersed into the particle of hundreds of nanometer sizes thus.
According to another embodiment of the invention, a kind of method of disperseing phytosterol is provided, it comprises the steps: under 60-200 ℃ of temperature, mixture heat fusing with phytosterol and emulsifying agent, described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester; Mixture and the water-based beverage of fusing or the water-based beverage that contains emulsifying agent are mixed; And high-speed stirred and homogenate mixture to be to obtain the beverage that phytosterol disperses, and phytosterol can be dispersed into the particle of hundreds of nanometer sizes thus.
According to another embodiment of the invention, a kind of method of disperseing phytosterol is provided, it comprises the steps: under 60-200 ℃ of temperature, with the mixture heat fusing of phytosterol and at least a emulsifying agent, described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester; The cooling melt solidifies, and abrasive solid gets powder, and powder and water-based beverage or the water-based beverage that contains emulsifying agent are mixed; And the high-speed stirred mixture to be to obtain the beverage that phytosterol disperses, and phytosterol can be dispersed into the particle of hundreds of nanometer sizes thus.
According to another embodiment of the invention, a kind of method of disperseing phytosterol is provided, it comprises the steps: under 60-200 ℃ of temperature, with the mixture heat fusing of phytosterol and at least a emulsifying agent, described emulsifying agent is selected from sucrose fatty ester, sorbitan fat acid esters and polyglyceryl fatty acid ester; The cooling melt solidifies, and abrasive solid gets powder, and powder and water-based beverage or the water-based beverage that contains emulsifying agent are mixed; And high-speed stirred and homogenate mixture to be to obtain the beverage that phytosterol disperses, and phytosterol can be dispersed into the particle of hundreds of nanometer sizes thus.
According to another embodiment of the invention, provide a kind of beverage that disperses with the phytosterol of one of said method preparation.
According to another embodiment of the invention, a kind of additive that is applicable to beverage is provided, it is characterized in that, this additive prepares as follows: with the mixture of phytosterol and at least a emulsifying agent at 60-200 ℃ of following heat fusing, form size in the time of in being scattered in water-based beverage and be hundreds of nanometers or littler particle, described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester.
Implement best mode of the present invention
On the naturally occurring phytosterol structure similar in appearance to cholesterol.At occurring in nature, found the phytosterol of numerous species, wherein sitosterol (sitosterol), campesterol (campesterol), stigmasterol (stigmasterol) and sitostamol (sitostanol) are more than other sterols.By common picked-up level, phytosterol is very little to the influence of serum cholesterol content, but a large amount of phytosterols can suppress the absorption of intestines inner cholesterol and biliary cholesterol, and this is owing to their structure and the structural similarity of cholesterol, thereby reduces the cholesterol level of serum.
Phytosterol has carried out research for a long time to the influence that reduces serum cholesterol.These studies show that phytosterol can reduce total serum cholesterol level 0.5-26%, particularly reduce LDL-C content 2-33%, and its reduction level depends on the diet of the order of severity and the regulation of dosage, patient symptom.The effect that cholesterol level reduces also is subjected to patient's sex, age and the health status and the influence of form of medication (suspension, crystalline solid, micelle etc.).
Now, generally accepted hypothesis is, phytosterol is assembled the cholesterol in the diet effectively, and these cholesterol exist and sedimentation with oil phase in intestines, thereby suppresses the absorption of intestines to dietary cholesterol exposure.Therefore, phytosterol it is believed that the cholesterol to existing with the micelle form is invalid.In fact, the report dietary cholesterol exposure is arranged than the be absorbed with more multiresistance (Mattson of interior living cholesterol to phytosterol, F.H., Volpenhein, R.A. and Erickson, B.A., Effect of PlantSterol Esters on the Absorption of Dietary Cholesterol, J.Nutr.1977; 107:1139-1146).But U.S. Patent number 5,932,562 adopt phytosterol to carry out once experiment, use on a small quantity even it is thought, and the micelle mixture of phytosterol also is very effective to the content that reduces serum cholesterol.Thereby the micelle mutual-assistance cholesterol that it is believed that phytosterol is deposited in the absorption that micelle effectively suppresses intestines in mutually well.
Up to now, utilize phytosterol to reduce in the blood especially LDL-content of cholesterol of content of cholesterol, developed many commercially produced products, these products are sold in many countries such as Finland, Britain, the U.S. and big Leah difficult to understand.They are mainly as tablespread or flavouring, and wherein phytosterol is by ester bond and aliphatic acid bonding.It is reported that these products and statin drug regimen use better to patient's curative effect of hypercholesterolemia.But this product contains a large amount of fat components, so that also is absorbed simultaneously.
Provide phytosterol to carry out many trials with drink form.But because phytosterol is slightly soluble in water, most of drink forms of being developed so far have following shortcoming: the biological utilisation rate variance of phytosterol, to lack dosage harmonious and need with a large amount of solubilizer.In addition, reach the absorption that suppresses cholesterol with the form of the conventional aqueous matrix of phytosterol, need the significantly long time, this is because their utilization rates in vivo are very slow.Further, the dispersion stabilization of phytosterol is very poor, so that the solubilizer that also needs drying process to remove to be used to disperse phytosterol water for example, thereby obtains the phytosterol of powdery.For fear of these problems, advised using various solutions, comprise with phytosterol grind to form tiny powder, add at least two kinds of different additives and use high pressure homogenizer (5,000psig).
As mentioned above, various solubilizer and emulsifying agent have been used for dissolving phytosterol, and according to U.S. Patent number 5,932,562 reports by form micelle from the organic solution of sitosterol, can easily disperse sitosterol.Generally speaking, because very big to the taste and the fragrance influence of beverage, the micelle mixture of this technology and gained is not suitable for beverage.For example, a large amount of solubilizer or emulsifying agent have bad influence to the taste of final consumable products.In addition, usually in food inapplicable organic solvent may stay in the micelle.
Therefore the present invention relates to the method for phytosterol being disperseed to become the micelle of hundreds of nanometer sizes, this method improvement the bioavailability of slightly soluble phytosterol, have suitable dosage harmony, and make the dispersion stabilization maximization.
According to the present invention, the bioavailability of phytosterol is greatly improved, and causes effective dose to reduce.In addition, acquisition be the phytosterol dispersion of clarifying fully, to the taste of purpose beverage and not influence of fragrance.Because micelle particle of the present invention is very little so that use the back is not bristly to mouthfeel.Another advantage of the present invention is that the beverage for preparing this reduction cholesterol need not be considered their pH value and composition.
Useful phytosterol is selected from sitosterol, campesterol, stigmasterol, sitostamol, campestanol (campestanol) and composition thereof among the present invention.In addition, the other plant sterol also can be used among the present invention.
According to process for dispersing of the present invention, can size be that hundreds of nanometers or littler micelle form disperse the example of the emulsifying agent of phytosterol to comprise sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester.Other emulsifying agents except sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester find that the size of generation significant quantity is 1 micron or bigger particle, as being measured by various experiment.In fact, other emulsifying agents show so low dispersion stabilization so that phytosterol are disperseed just to produce in back three days precipitation.Therefore, other emulsifying agents except that sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester emulsifying agent are not suitable for the preparation beverage on substantially.Although stearoyl lactate is optimum aspect dispersion stabilization, because its security can not get ensureing that use or the consumption to it has strict restriction legally.In addition, this emulsifying agent has distinctive unpleasant taste.
In contrast, the used emulsifying agent of the present invention makes phytosterol disperse with hundreds of nanometers or littler size.And this dispersion stabilization is fine, so that the dispersion physical efficiency of 1% phytosterol was preserved 1 year or the longer time.In the sucrose fatty ester preferably hydrophilic-lipophilic balance value (HLB) be 7 or bigger those.Their HLB value is more preferably between 10 to 16.Sorbitan aliphatic ester preferably has the HLB value between 5 to 10, more preferably between 7 to 10.As for polyglyceryl fatty acid ester, its HLB value is preferably between 10 to 20, and more preferably between 12 to 15.Littler than other emulsifying agent sucrose fatty esters and polyglyceryl fatty acid ester gained particle with sorbitan aliphatic ester as emulsifying agent gained particle, and particle size distribution is more even.In addition, these esters distribute some unpleasant tastes.In a word, sucrose fatty ester is most preferred.
In dispersion of the present invention, the weight ratio of phytosterol and whole emulsifying agents is 1: the 0.01-10 scope, and preferably 1: 0.2-2.0 (w/w) scope.For example, if the weight ratio of emulsifying agent and phytosterol is lower than 0.01, just emulsification and produce precipitation fully, even if form the emulsus particle, its granular size also has tens of microns big.On the other hand, if its weight ratio surpasses 10, the taste of gained beverage is the taste of emulsifying agent, and mouthfeel is too poor.Compare with the emulsifying agent that is mixed with phytosterol as for this emulsifying agent that is mixed with water-based beverage, its weight ratio be 0.8 or littler (promptly be 80% or littler by weight, weight based on the emulsifying agent that is mixed with phytosterol), preferred weight ratio is 0.5 or littler (promptly being 80% or littler by weight).Weight ratio makes it be difficult to form nano particle greater than 0.8 (w/w) (being 80% by weight), and this is because the amount that is mixed with the emulsifying agent of phytosterol is low relatively.
Various water-based beverages can be used among the present invention, for example water, fruit drink, carbonated drink, milk, soymilk, cereal beverage arranged, other popular beverages such as coffee, green tea, liliaceous plant radix polygonati officinalis variant (polygonatum odoratum var.) tea etc., and alcoholic beverage.
According to the present invention, at 60-200 ℃ with phytosterol and emulsifier.The heating-up temperature of mixture is preferably in 120-150 ℃ of scope.When being blended in when carrying out when being lower than 60 ℃, the micelle granular size is tens of to hundreds of microns, and this is unfavorable for mouthfeel and bioavailability.On the other hand, mixing temperature is higher than 200 ℃, although phytosterol is still stable in the time of 250 ℃, emulsifying agent is sex change.
Substantially, having in the presence of the emulsifying agent, the emulsification of difference during emulsification, only takes place in the material phytosterol that is slightly soluble in water in water, cause phytosterol to be deposited to size for tens of to hundreds of microns particle.In the present invention, deep research relates to the emulsification maximization that makes phytosterol, is hundreds of nanometers or littler micelle particle thereby obtain size.Found that of further investigation, when the mixture of phytosterol and emulsifying agent such as sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester or these emulsifying agents was mixed into homogeney, emulsification just can maximize.In order to make phytosterol and emulsifier even, with near phytosterol (sitosterol: about 140 ℃ its fusing point; Campesterol: about 157 ℃; Stigmasterol: about 170 ℃) heating becomes after the liquid state these two kinds of compositions and mixes.
Under high-speed stirred, water-based beverage or the water-based beverage that contains emulsifying agent are joined in the hot mixt of phytosterol and emulsifying agent.The emulsifying agent that adds in the water-based beverage is preferably the same with the emulsifying agent that is mixed with phytosterol.But,, also can use different emulsifying agents if two kinds of different emulsifying agents are compatibility each other.The weight ratio of phytosterol and water-based beverage exists, and 1: 10 to 1: 10, between 000 (w/w) scope, and preferably between 1: 10 to 1: 100 (w/w) scope.
The example of available water-based beverage comprises water, fruit drink, carbonated drink, milk, soymilk, cereal beverage, other popular beverages such as coffee, tea etc. among the present invention, and alcoholic beverage, and more is partial to water.Make under the situation of water, by further use beverage such as water, fruit drink, carbonated drink, milk, soymilk, cereal beverage, other popular beverages and the alcoholic beverage dilution of dispersion of the phytosterol of subsequently high-speed stirred and homogenate process gained, thereby obtain the required beverage that contains phytosterol.
For guaranteeing constant product quality, obtain the size distribution homogeneous granules, industrial, high-speed stirred and homogenate technology are important.
Perhaps, also phytosterol and emulsifier can be heated near its fusing point, the cooling melt gets solid, then solid abrasive is become powder.By simply powder being added water-based beverage or containing in the beverage of emulsifying agent, just made the beverage that contains phytosterol.With respect to liquid form, the advantage that the additive of powder type has is that they are convenient to operate, are difficult for by microbial contamination and safer and have low logistics costs and be easy to transportation in when transportation.
When with water when the water-based substrate, after the mixture of phytosterol and emulsifying agent was dispersed in the water, the dispersion of gained was through pervaporation, freeze-drying or spray-drying, thereby made water-based phytosterol powder.Then this powder is used for water-based beverage so that the beverage that contains phytosterol to be provided.
Phytosterol and sucrose fatty ester are added in the entry by the mixture of heating gained before mixing, carry out stirring technique and high-pressure homogenization technology to obtain transparent phytosterol dispersion with predefined procedure then.When the consumption of phytosterol was 1%, conventional emulsion process just can not guarantee to cause the sedimentation of phytosterol by the dispersion stabilization of gained solution.The conventional dispersion that emulsion process produced is low to moderate 0.16% at 700nm place light transmittance, and according to method of the present invention, the light transmittance at the 700nm place is 80.0% or higher.
About the mixture of phytosterol and emulsifying agent and mixing of water-based beverage, the mixture of phytosterol and emulsifying agent can be joined in the water-based beverage with the liquid phase of heat or the solid phase form that is chilled to room temperature.Under the former situation, water-based beverage is heated to 60-140 ℃, preferably is heated to 70-90 ℃, to strengthen its emulsifying power.The optimum temperature that liquid phase mixture directly adds in the beverage is 80 ℃.Particularly, in order to obtain little micelle particle, the heating-up temperature of water-based beverage is preferably set to similar to the temperature of the mixture of phytosterol and emulsifying agent.In water, after the emulsification, need pressurization to make temperature rise to 100 ℃.For example, make the 140 ℃ of following emulsifications in water of phytosterol mixture just need about 5 atmospheric pressure.
Mixture has just formed nano particle after stirring.In this, stir with 5,000-10,000rpm is preferably 6, and 500-7,500rpm carried out about 10 minutes.Behind the stirring technique micelle of gained after tested, wherein 90% or the size of more particles be 300nm or littler.In contrast, except the mixture of phytosterol and emulsifying agent is not heated, under same condition the gained micelle after measured, its granular size is tens of scopes to hundreds of microns.Therefore, these measurement results relatively show, are crucial with phytosterol and emulsifying agent fusing and the technology of mixing them to forming nano particle.In addition, as hereinafter describing, high-speed stirred or homogenate technology also are important to forming particle of uniform size.
Do not having in the presence of other compositions, after heating, can make phytosterol and emulsifying agent even contact each other, fusing, so that the micelle granular size of gained is hundreds of nanometers after the emulsification.Opposite with routine techniques, the present invention can just can make the nano particle that is suitable for using not adopting phytosterol wherein still under the situation of soluble organic solvent in beverage.
After the stirring, need homogenate technology that the micelle of assembling is pulverized.This homogenate technology can be undertaken by the wherein preferred high pressure homogenizer of high pressure homogenizer, rubber mill or ultrasonoscope.According to the present invention, with 2,000-25, the pressure of 000psi be preferably with 7 in Syrup-homogenizing instrument for micelle, 000-10, and the pressure of 000psi carries out homogenate.Through this step process, measure the gained micelle, 95% or more micelle granular size at 300nm or littler.
According to the present invention, by heat fusing phytosterol and emulsifying agent, mix, stir the mixture in water and processing under high pressure, dispersions obtained beverage or other popular beverages made from fruit drink, carbonated drink, milk, soymilk, cereal diluted the beverage that contains phytosterol that has just obtained wanting.In these beverages, the micelle granular size is little of nanometer, has big surface area and particle curvature, and excellent bioavailability does not also influence the peculiar taste and the fragrance of beverage.
In addition, even also can not carry out layering when storing in refrigerator according to the made beverage of the present invention, this is because improved the dispersion stabilization of phytosterol micelle.Main is that this phytosterol micelle keeps fabulous dispersion stabilization in the time of 90 ℃, and this just guarantees the product long-term stability.
Just can understand the present invention better according to following embodiment, described embodiment is intended to explanation and can not be construed as limiting the invention.Among the embodiment, (MalvernInstrument LTD. UK) analyzes particle size distribution with Mastersizer below.
Comparative example 1
In 1 liter of container, add 500 gram water, be heated to about 80 ℃, then 5 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and 4.25 gram sucrose stearates (HLB 11) are added in the hot water, then with 6,800-7, the speed of 000rpm stirred this mixture 10 minutes.Analyze the gained granular size, it the results are shown in the following table 1.
Table 1
Granular size (μ m) Accumulation %
0.985 0.07
1.89 12.41
2.50 32.52
3.31 53.23
4.38 66.82
5.27 77.28
6.35 85.32
11.11 95.69
21.32 98.96
78.56 100.00
Comparative example 2
The dispersion that makes by comparative example 1 with as by Microfluidics make, model for the high pressure homogenizer of " Microfluidizer M110EHI " with 7, the pressure of 000psi is handled through a passage.Analyze the gained granular size, it the results are shown in the following table 2.Mensuration is dispersions obtained at the 700nm place, and its light transmittance is 0.16%.
Table 2
Granular size (μ m) Accumulation %
0.985 0.03
1.89 11.25
2.50 30.43
3.31 54.47
4.38 66.55
5.27 79.74
6.35 88.45
11.11 96.21
21.32 99.46
94.65 100.00
Comparative example 3
The 40 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol), 36 of packing in 1 liter of container restrain vegetable oil and 4 gram polysolvate 60 (HLB 14.9), make it be melted to clarification under 130-140 ℃ of stirring.After fusing is finished, 320 grams are maintained at about 80 ℃ water add in this limpid melt, then with about 10, the speed stir about of 000rpm 10 minutes.As if observing floatingly on the phytosterol dispersion of gained has the phytosterol particle, and a large amount of phytosterol particle is attached on the chamber wall.Particle size distribution (volume) to dispersion is analyzed, and the result is 1 μ m or bigger for 91% or more its granular sizes of sum, and approximately sum 81% or more mostly be 100 μ m or bigger.In addition, also observe and occur sedimentation and lamination at room temperature 1 hour, be sure of that this phytosterol dispersion can not be used for beverage.
Comparative example 4
The 15 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and 30 of packing in 1 liter of container restrain citric acid monoglycerides (HLB 8.0), make it be melted to clarification under 130-140 ℃ of stirring.The water that will be maintained at about 80 ℃ subsequently adds in this limpid melt, then with about 6, and the speed stir about of 800rpm 10 minutes.Gained solution with as by Microfluidics make, model be the high pressure homogenizer of " Microfluidizer M110EHI " with 10, the pressure of 000psi is handled through a passage.In the phytosterol that so obtains, can be clear that a large amount of phytosterol particles is attached on the chamber wall, this be since the phytosterol dispersion not with protein combination but combine with water.When the concentration of phytosterol was 1%, the viscosity of this dispersion was up to 58cps.Particle size distribution (volume) to this dispersion is analyzed, and the measurement result of dispersion is for being 68% or when higher, granular size is 1 μ m or bigger when accumulative perception; When accumulative perception is 21% or when higher, granular size is 10 μ m or bigger, so big particle is enough to produce bristly mouthfeel.In addition, its dispersion stabilization is very poor, causes this dispersion to be difficult to use in the preparation beverage.
Comparative example 5
The 15 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and 15 of packing in 1 liter of container restrain stearoyl lactates (SSL), make it be melted to clarification under 130-140 ℃ of stirring.Subsequently 300 grams are maintained at about 80 ℃ water and add in this limpid melt, then with about 6, the speed stir about of 800rpm 10 minutes.Gained solution with as by Microfluidics make, model be the high pressure homogenizer of " Microfluidizer M110EHI " with 10, the pressure of 000psi is handled through a passage.Observation of plant sterol dispersion, it precipitated in one day, finished curing in three days, and these results cause this dispersion to be difficult to use in the preparation beverage.Particle size distribution (volume) to this dispersion is analyzed, and the measurement result of dispersion is for being 57% or when higher, granular size is 1 μ m or bigger when accumulative perception; When accumulative perception is 17% or when higher, granular size is 10 μ m or bigger.So poor dispersion stabilization causes this dispersion to be difficult to use in the preparation beverage.
Embodiment 1
Pack in a container phytosterol (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and sucrose stearate (HLB 11) and/or sorbitan bay alcohol ester (HLB 8.6) make its fusing under 130-140 ℃ of stirring.After fusing was finished, solution stirred 1 minute again, join in the water that is maintained at about 80 ℃, and then with 6,800-7, the speed stir about of 000rpm 10 minutes.Gained solution with as by Microfluidics make, model be the high pressure homogenizer of " Microfluidizer M110EHI " with 7, the pressure of 000psi is handled through a passage.Table 3 has been listed the consumption of phytosterol, sucrose stearate and sorbitan bay alcohol ester, specifies and whether carries out high-pressure homogenization technology.
Table 3
Numbering Phytosterol Sucrose stearate Sorbitan bay alcohol ester Water High-pressure homogenization
1 5g 4.25g - 500g Not
2 5g 4.25g - 500g Be
3 5g 3.036g 1.214g 500g Not
4 5g 3.036g 1.214g 500g Be
5 25g 2.5g 1.75g 500g Not
6 25g 2.5g 1.75g 500g Be
7 25g 2.126g 2.124g 500g Not
8 25g 2.126g 2.124g 500g Be
The solution of numbering 1 carries out the particle size distribution analysis in the his-and-hers watches 3, and it the results are shown in the following table 4.
Table 4
Granular size (μ m) Accumulation %
0.096 20.35
0.127 52.19
0.153 68.49
0.184 75.29
0.222 85.33
0.294 91.52
0.985 99.21
5.27 100.0
Be reference purpose, the analysis result that is numbered 3,5,7 particle in the table 3 is similar to the result of the numbering 1 shown in the table 4.
Under the situation of numbering 2, the analysis result of particle is listed in the following table 5 in table 3.
Table 5
Granular size (μ m) Accumulation %
0.096 13.67
0.127 49.40
0.153 69.39
0.184 77.61
0.222 89.07
0.294 95.22
0.985 99.89
2.08 100.0
Be reference purpose, it is similar to the result of numbering 2 shown in the table 5 to number 4,6,8 analysis result in the table 3.
The phytosterol dispersion (numbering 2,4,6,8) of embodiment 1 preparation is measured its light transmittance at the 700nm place, scope is 80.0% to 80.5%.
Embodiment 2
Pack in a container phytosterol (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and sucrose stearate (HLB 11) and/or sorbitan bay alcohol ester (HLB 8.6) make its fusing under 130-140 ℃ of stirring.After fusing was finished, solution stirred 1 minute again, add in the water (80-90 ℃) that contains 1 gram sucrose stearate, and then with 6,800-7, the speed stir about of 000rpm 10 minutes.(Microfluidizer M110EHI, Microfluidics) with 7, handle through a passage by the pressure of 000psi with high pressure homogenizer for gained solution.Table 6 has been listed the consumption of phytosterol, sucrose stearate and sorbitan bay alcohol ester, specifies and whether carries out high-pressure homogenization technology.
Table 6
Numbering Phytosterol Sucrose stearate Sorbitan bay alcohol ester Water High-pressure homogenization
9 5g 4.25g - 500g Not
10 5g 4.25g - 500g Be
11 5g 3.036g 1.214g 500g Not
12 5g 3.036g 1.214g 500g Be
13 25g 2.5g 1.75g 500g Not
14 25g 2.5g 1.75g 500g Be
15 25g 2.126g 2.124g 500g Not
16 25g 2.126g 2.124g 500g Be
The solution of numbering 9 carries out the granular size analysis in the his-and-hers watches 6, and it the results are shown in the following table 7.
Table 7
Granular size (μ m) Accumulation %
0.096 19.21
0.127 52.30
0.153 68.72
0.184 76.41
0.222 85.95
0.294 92.05
0.985 99.35
4.80 100.0
Be reference purpose, number 11,13 similar to the result of numbering 9 shown in the table 7 in the table 6 with the analysis result of 15 particle.
Under the situation of numbering 10, grading analysis the results are shown in the following table 8 in table 6.
Table 8
Granular size (μ m) Accumulation %
0.096 14.50
0.127 48.24
0.153 70.68
0.184 77.92
0.222 90.61
0.294 96.74
0.985 99.85
1.89 100.0
Be reference purpose, number 12,14 similar to the result of the numbering 10 shown in the table 8 in the table 6 with the analysis result of 16 particle.
Measure the light transmittance of the phytosterol dispersion (numbering 10,12,14 and 16) of embodiment 2 preparations at the 700nm place, its scope is 80.5% to 82.5%.
Embodiment 3
The 5 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and 4.25 of packing in a container restrain polyglycereol monostearates (HLB 12), make its fusing under 130-140 ℃ of stirring.After fusing was finished, melt stirred 1 minute again, and it is joined 490.75 grams was heated in 80 ℃ the water, then with 6, and 800-7, the speed stir about of 000rpm 10 minutes.(Microfluidizer M110EHI, Microfluidics) with 7, handle through a passage by the pressure of 000psi with high pressure homogenizer for gained solution.
Before the high-pressure homogenization, in the experimental error that allows, the analysis result of granular size is identical with table 4.
After the high-pressure homogenization, in the experimental error that allows, the analysis result of granular size is identical with table 5.
After the high-pressure homogenization, measure the light transmittance of phytosterol dispersion at the 700nm place, its scope is 80.0% to 80.5%.
Embodiment 4
The 5 gram phytosterols (75% sitosterol, 10% campesterol, 15% stigmasterol sitostamol) and 3.25 of packing in a container restrain polyglycereol monostearates (HLB 12), make its fusing under 130-140 ℃ of stirring.After fusing is finished, melt thing and stir 1 minute again, and join 491.25 grams and contain in the water (80-90 ℃) of the hard acyl ester of 1 gram polyglycereol list, then with 6,800-7, the speed stir about of 000rpm 10 minutes.(Microfluidizer M110EHI, Microfluidics) with 7, handle through a passage by the pressure of 000psi with high pressure homogenizer for gained solution.
Before the high-pressure homogenization, in the experimental error that allows, the analysis result of granular size is identical with table 7.
After the high-pressure homogenization, in the experimental error that allows, the analysis result of granular size is identical with table 8.
After the high-pressure homogenization, measure the light transmittance of phytosterol dispersion at the 700nm place, its scope is 80.0% to 82.5%.
Embodiment 5
Thereby the dispersion for preparing among the embodiment 1-4 is carried out spray-drying obtain water-based phytosterol powder.
Embodiment 6
The 100 gram dispersions that prepare among each embodiment 1-4 are diluted with 400 gram water-based beverages, and described water-based beverage is selected from beverage and other popular beverages that water, fruit drink, carbonated drink, milk, soymilk, cereal are made.9.25 gram powder of preparation among the embodiment 5 are added in the 490.75 gram water-based beverages, and described water-based beverage is selected from beverage and other popular beverages that water, fruit drink, carbonated drink, milk, soymilk, cereal are made.50 professional technique experimenters (20 ages are the male sex of one's late 30s and more than 40 year old, and 20 ages are in the women of one's late 30s and more than 40 year old, 10 end wedding women) take the dilution beverage of gained, to feel to test its performance.With fruit juice (orange juice) beverage as a comparison.It the results are shown in following table 9 and the table 10.
Table 9
Integration test
Be better than conventional beverage The same with conventional beverage Not as good as conventional beverage
Number 9 40 1
Percentage 18％ 80％ 2％
Table 10
Flavor tests
Sensation Very approval Approval Neutral Do not admit Very do not admit
Acid 2 6 30 11 1
Sweet 1 3 43 2 1
Bitter 0 1 2 5 42
The sense of tool bristle 0 0 0 3 47
When the test Other Drinks, the gained result is similar with table 10 to table 9.
Embodiment 7
The beverage of preparation among the embodiment 6 is placed on storage 1 year or longer time in the refrigerator (4 ℃).Individually, same beverage was handled 4 hours down at 90 ℃.Not only observe this beverage and keep normal dispersed, and do not find other difference.
Embodiment 8
The 5 gram phytosterols and 4.25 of packing in a container restrain sucrose stearates (HLB11), melt under 130-140 ℃ of stirring.After fusing is finished, stirred again 1-2 minute.Add 490.75 gram water-based beverages in gained solution, described water-based beverage is selected from beverage and other popular beverages that water, fruit drink, carbonated drink, milk, soymilk, cereal are made.50 professional technique experimenters (20 ages are the male sex of one's late 30s and more than 40 year old, and 20 ages are in the women of one's late 30s and more than 40 year old, 10 end wedding women) take this dilution beverage, to feel to test its performance.With fruit juice (orange juice) as a kind of contrast beverage.It the results are shown in following table 11 and the table 12.
Table 11
Integration test
Be better than conventional beverage The same with conventional beverage Not as good as conventional beverage
Number 6 42 2
Percentage 12％ 84％ 4％
Table 12
Flavor tests
Percentage Very approval Approval Neutral Do not admit Very do not admit
Acid 3 5 31 10 1
Sweet 1 3 43 2 1
Bitter 0 1 3 4 42
The sense of tool bristle 0 0 1 4 45
When the test Other Drinks, the gained result is similar with table 12 to table 11.
Embodiment 9
The 5 gram phytosterols (fusing point: 143 ℃) and 4.25 of packing in a container restrain sucrose stearates (HLB 11), make its fusing under 130-140 ℃ of stirring.After the fusing, stirred again 1-2 minute fully.The even melt of gained is chilled to room temperature, gets solid, then solid abrasive is become powder.
Embodiment 10
Such as melting point detector (Electrothermal 9200) mensuration, among the embodiment 9 preparation its fusing point of powder be 125 ℃.
Embodiment 11
9.25 powders that restrain preparation in embodiment 5 or 9 are added to 90.75 restrain in the water that is heated to 80-90 ℃, with 6,800-7, the speed of 000rpm stirred 10 minutes.
Embodiment 12
(MicrofluidizerM110EHI, Microfluidics) with 7, handle through a passage by the pressure of 000psi with high pressure homogenizer for the solution that will make in embodiment 11.
Embodiment 13
100 gram dispersions of preparation in embodiment 11 or 12 are diluted with 400 gram water-based beverages, and described water-based beverage is selected from beverage and other popular beverages that water, fruit drink, carbonated drink, milk, soymilk, cereal are made.
Embodiment 14
9.25 gram dispersions of preparation in embodiment 5 or 9 are added in the 490.75 gram water-based beverages, described water-based beverage is selected from beverage and other popular beverages that water, fruit drink, carbonated drink, milk, soymilk, cereal are made, and with 6,800-7, the speed stir about of 000rpm 10 minutes.
Embodiment 15
The beverage of preparation among the embodiment 14 is used high pressure homogenizer, and (MicrofluidizerM110EHI, Microfluidics) with 7, the pressure of 000psi is handled through a passage.
Embodiment 16
Store 1 year under the beverage room temperature with preparation in embodiment 14 and 15 or store 1 year in longer time, refrigerator (4 ℃) or longer time and 90 ℃ were placed 4 hours down, monitor its physical state simultaneously.Not only observe beverage and keep normal dispersed, and do not find other difference.
Embodiment 17
Clinical testing
According to the method for embodiment 4, numbering 2 dispersion among the embodiment 1 mixed with coffee, milk or green tea and must beverage be used for clinical testing.
1. clinical experimenter: 45 people suffer from slight hyperlipidemia.
Table 13
Experimenter's situation before clinical testing
Surname is (man: the woman) not 15∶30
Age (year) 56.53±10.28
Constitutional index (BMI) (kg/m 2) 25.14±2.51
Ideal body weight percentage (PIBW) 119.20±12.60
Total serum cholesterol (mg/L) 246.12±27.61
Neutral lipid (mg/L) 145.81±57.43
LDL-cholesterol (mg/L) 161.90±19.35
HDL-cholesterol (mg/L) 52.89±12.54
2. clinical experimenter's diet control: no special diet; Provide low-cholesterol diet to the patient who suffers from slight hyperlipidemia.
Table 14
Clinical experimenter's eating condition
Total amount of heat (Cal) 1705.9±330.49
Albumen (g) 64.26±18.53
Total lipid (g) 40.12±17.26
Saturated lipid (g) 11.12±6.13
Cholesterol (mg) 135.82±80.13
3. write out a prescription
Be used for clinical testing with every bottle of green tea or coffee that contains 1.6 gram phytosterols.
3-1 test group 1
Step 1: the from the 0th to the 4th week, every day 1 bottle of beverage.
Step 2: the from the 5th to the 8th week, every day 2 bottles of beverages.
3-2 test group 2
Step 1: the from the 0th to the 4th week, every day 1 bottle of placebo.
Step 2: the from the 5th to the 8th week, every day 1 bottle of beverage.
Step 3: the from the 9th to the 12nd week, every day 2 bottles of beverages.
4. result
45 people (men: woman=15: 30, mean age: 56) carry out according to the explanation and the condition of clinical testing fully.Incipient stage, measure clinical experimenter and take in 11.12 gram saturated fat and 135.8 milligrams of cholesterol with per day diet.In taking in placebo test group and 8 weeks absorption phytosterol test group, observe result of treatment with total serum cholesterol (p=0.039) and LDL-cholesterol (p=0.036) content.In these groups, the cycle of serum lipid content (T-CHOL, neutral lipid, HDL-cholesterol, LDL-cholesterol) is not significant on the statistics with leaving over effect.Taking in phytosterol after 8 weeks, measure patient, its total cholesterol level reduces by 4.38% (p=0.039), and the LDL-cholesterol level reduces by 8.28% (p=0.036).In clinical experimenter, 33 people's total cholesterol level on average reduces by 9.2%, and 31 people's LDL-cholesterol level on average reduces by 14.1%.
5. conclusion
Hyperlipidemia patient just can reduce total serum cholesterol and LDL-content of cholesterol taking in the beverage that contains phytosterol of the present invention after 8 weeks.This reduction effect can obtain from the patient who takes in low dietary cholesterol exposure.
Industrial applicibility
As mentioned above, according to the present invention, the formation method of phytosterol nano particle is that phytosterol and at least a emulsifying agent are carried out heat fusing, described emulsifying agent is selected from S-1670, sorbitan aliphatic ester and polyglyceryl fatty acid ester, and the mixture of this fusing is dispersed in the water-based substrate. The beverage that contains phytosterol of the present invention can suppress in the intestines and the absorption of biliary cholesterol, thereby reduces the content of serum cholesterol. According to the present invention, need not organic solvent, just to make granular size be hundreds of nanometers or less phytosterol micelle, and therefore can be used in the water-based beverage. Because phytosterol micelle of the present invention is that hundreds of nanometers or less particle form by size, this micelle has higher bioavailability. In addition, the dispersion stabilization of raising phytosterol micelle can prolong the beverage life cycle, guarantee that product is stable for a long time. Further, this micelle can needn't be considered for nearly all beverage matrix and the pH value of beverage. Because this micelle does not affect the distinctive taste of beverage and fragrance, and good mouthfeel, therefore can be enough to be used in the various water-based beverages.

Claims (38)

1. method of disperseing phytosterol, it comprises the following steps:
Mixture with phytosterol and at least a emulsifying agent under 60-200 ℃ carries out heat fusing, and described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester;
The mixture that makes fusing and water-based beverage or the water-based beverage that contains emulsifying agent mix; And
With 5,000 to 10, the speed of 000rpm stirs the dispersion of this mixture with generation phytosterol in beverage,
Thus phytosterol can be dispersed into 90% or more particles size be the particle of 300nm.
2. the method for claim 1 further comprises step:
With 5,000 to 10, the speed of 000rpm stirs after the step of this mixture 2,000～25, this mixture of homogenate under the pressure of 000psi.
3. the method for claim 1 is characterized in that described method comprises the following steps:
Between heat fusing step and the step that stirs the mixture, make melt be cooled to solid,, and powder and water-based beverage or the water-based beverage that contains emulsifying agent mixed the solid pulverize.
4. the method for claim 3 further comprises the following steps:
With 5,000 to 10, the speed of 000rpm stirs after the step of this mixture 2,000 to 25, this mixture of homogenate under the pressure of 000psi.
5. as the method for claim 1-4 described in each, wherein 95.0% or the size of more particles be 300 nanometers or littler.
6. the method described in claim 1 or 2, it comprises that further dry dispersion is to produce the step of water-based phytosterol powder.
7. the method described in claim 6, wherein drying steps comprises evaporation or freeze drying or spray-drying.
8. as the method for claim 1-4 described in each, wherein phytosterol is to be selected from least a in sitosterol, campesterol, stigmasterol, sitostamol and the campestanol.
9. the method described in claim 8, wherein phytosterol is a sitosterol.
10. as the method for claim 1-4 described in each, wherein emulsifying agent is a sucrose fatty ester.
11. the method described in claim 10, wherein the hydrophilic-lipophilic balance value of sucrose fatty ester is 7 or higher.
12. the method described in claim 11, wherein the hydrophilic-lipophilic balance value of sucrose fatty ester is 10-16.
13. as the method for claim 1-4 described in each, wherein whipping step is with 6,500-7, and the speed of 500rpm is carried out.
14. as the method for claim 1-4 described in each, wherein water-based beverage is the beverage made of water, fruit drink, carbonated drink, milk, soymilk, cereal, beverage or the alcoholic beverage that other people liked.
15. the method described in claim 14, wherein water-based beverage is a water.
16., wherein melt step and under 120-150 ℃, carry out as the method for claim 1-4 described in each.
17. as the method for claim 1-4 described in each, wherein with phytosterol and total emulsifying agent with weight ratio 1: 0.01-10 mixes.
18. the method described in claim 17, wherein with phytosterol and total emulsifying agent with weight ratio 1: 0.2-2.0 mixes.
19. as the method for claim 1-4 described in each, wherein the consumption of the emulsifying agent that mixes mutually with water-based beverage is 80% or still less according to the weight that is mixed with the emulsifying agent of phytosterol by weight.
20. as the method for claim 1-4 described in each, wherein with phytosterol and water-based beverage with weight ratio 1: 10-10,000 (w/w) mixes.
21. the method described in claim 20 is wherein mixed with water-based beverage phytosterol with weight ratio 1: 10-100 (w/w).
22., wherein the mixture of phytosterol and emulsifying agent and the composition of water-based beverage are maintained under 60-140 ℃ as the method for claim 1-4 described in each.
23. the method described in claim 22 wherein maintains described composition under 70-90 ℃.
24. as the method for claim 1-4 described in each, wherein the light transmittance of dispersion at the 700nm place is 80.0% or higher.
25. the method described in claim 2 or 4, wherein homogenate step adopt high pressure homogenizer, rubber mill or ultrasonoscope to carry out.
26. the method described in claim 25, wherein homogenate step adopt high pressure homogenizer to carry out.
27. the method described in claim 2 or 4, wherein 7,000-10, the homogenate of 000psi operate high pressure.
28. the beverage that a plant sterols is disperseed, it adopts each described method preparation of claim 1-4.
29. the beverage that the phytosterol described in claim 28 disperses, wherein beverage is based on beverage, other popular beverages or the alcoholic beverage that water, fruit drink, carbonated drink, milk, soymilk, cereal are made.
30. additive that is applicable to beverage, it is characterized in that it prepares by phytosterol and at least a emulsifying agent are carried out heat fusing under 60-200 ℃, and work as it with 5,000-10,000rpm stirs, then with 2,000-25, the pressure of 000psi carry out homogenate and form size when being dispersed in the water-based beverage is 300 nanometers or littler particle, and described emulsifying agent is selected from sucrose fatty ester, sorbitan aliphatic ester and polyglyceryl fatty acid ester.
31. the additive described in claim 30, wherein 90% or the size of more particles be 300 nanometers or littler.
32. the additive described in claim 31, wherein 95% or the size of more particles be 300 nanometers or littler.
33. the additive described in claim 30, wherein the form of additive is a powder.
34. the additive described in claim 30 wherein heats mixture down at 130-140 ℃.
35. the additive described in claim 30, wherein phytosterol is to be selected from least a in sitosterol, campesterol, stigmasterol, sitostamol and the campestanol.
36. the additive described in claim 30, wherein emulsifying agent is a sucrose fatty ester.
37. the additive described in claim 36, wherein the hydrophilic-lipophilic balance value of sucrose fatty ester is 7 or higher.
38. the additive described in claim 37, wherein the hydrophilic-lipophilic balance value of sucrose fatty ester is 10-16.
CNB018165923A 2000-09-30 2001-09-28 Disperse the method for phytosterol and the beverage that contains nano level phytosterol Expired - Fee Related CN100553495C (en)

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