CN100549695C - A kind of measuring instrument of real-time human body blood viscosity - Google Patents
A kind of measuring instrument of real-time human body blood viscosity Download PDFInfo
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- CN100549695C CN100549695C CNB2005100642061A CN200510064206A CN100549695C CN 100549695 C CN100549695 C CN 100549695C CN B2005100642061 A CNB2005100642061 A CN B2005100642061A CN 200510064206 A CN200510064206 A CN 200510064206A CN 100549695 C CN100549695 C CN 100549695C
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Abstract
The present invention relates to a kind of measuring instrument of real-time human body blood viscosity.This measuring instrument comprises pressure source, woven hose, micron tube, transparent capillary, demarcation liquid container, temperature conditioning unit, DATA REASONING and processing unit, display module, the control module of demarcating liquid is housed; Transparent capillary is in DATA REASONING and processing unit inside, and relative measurement method is adopted in viscosity measurement.The measuring instrument energy clear operation and the reading error of real-time human body blood viscosity of the present invention, accuracy of detection and automaticity height; Blood using amount is less, only can measure with about 20ul blood; Woven hose, micron tube and transparent capillary are disposable use, avoid infection; Simple to operate, be easy to carry, be adapted at hospital clinical or average family and use the human body blood viscosity.
Description
Technical field
The present invention relates to a kind of measuring instrument of real-time human body blood viscosity.
Background technology
At medical domain, the viscosity of measuring blood and other physiological fluid is the diagnostic means that recent development is got up, and the diagnostic application to difficult and complicated illness such as angiocardiopathy and cancer, knurls is more extensive especially.But the instrumentation blood sampling volume complicated and needs that has medical detection blood viscosity now is bigger.
Existing blood viscosity measuring method mainly contains capillary tube technique, rotary process, falling bodies method, vibratory drilling method, flat band method etc.The simplest measuring method is a capillary tube technique, i.e. the Pinkevitch viscosity meter.For example: China Patent No. is 98239308.3, denomination of invention is in the file of " intelligent capillary blood viscosity meter ", the technical scheme of putting down in writing this viscosity meter comprises: a glass vertical tube is arranged, glass capillary, vertical tube and the glass capillary of the horizontal positioned that is connected with the lower port of vertical tube all place constant water bath box, being positioned at glass capillary outlet below has a load measuring sensor, and this sensor transmits signal by the A/D capture card to computing machine.Glass vertical tube inlet is communicated with a thicker plastic cement pipe.This pipe is more respectively with thinner sample introduction pipe, testing tube and dry up pipe and be communicated with.Sampling pump and solenoid valve are installed on sample introduction pipe and the purge pipe, have the test solenoid valve on the testing tube, the termination is the test blow vent, dries up to be equipped with successively on the pipe to dry up solenoid valve, dry up pump, and the termination is to dry up blow vent.The whole system operation principle is: in the test process, the liquid level of the liquid in the vertical tube constantly reduces, and the required driving pressure differential of flow of liquid also constantly reduces in the kapillary, just can obtain changing from high to low shear rate in view of the above, at capillary end a highly sensitive load measuring sensor is installed, can measure the discharge signal of moment continuously, again signal is input in the computing machine through the A/D capture card then and handles, so just can draw the whole blood surface viscosity on any point in the shear rate scope of regulation.
But this measuring method and the used internal diameter capillaceous of device are generally a millimeter magnitude, because internal diameter capillaceous is thick, just need to extract a large amount of blood during clinical use concerning patient, general desired blood liquid is more than 10ml, so this instrument is unsuitable for using at hospital clinical.The experimental temperature that second defective is this instrument is to control by constant water bath box; If the scope of measuring temperature is than broad, between 5～50 ℃, the cost height of calibration cell, secondly volume is bigger, also is unsuitable for using at hospital clinical.
Summary of the invention
The objective of the invention is to: it is bigger to overcome existing blood viscosity measuring instrument blood using amount, and common micro liquid device for measuring viscosity is not integrated as yet, is only applicable to be not suitable for being operated by the layman in surroundings by professional's use in the laboratory limitation of human body blood viscosity; Provide a kind of and be easy to carry, simple to operate, blood using amount is few, the measuring instrument of real-time human body blood viscosity.
For achieving the above object, the technical solution used in the present invention is as follows:
As shown in Figure 1, this measuring instrument comprises: a power supply 20 is connected with a pressure source 1, pressure source 1 is connected by first end of a threeway 2 with a woven hose 4, one is equipped with between the demarcation liquid container 5 of demarcating liquid and this threeway 2 and is connected by a valve 6, and this valve 6 is used for controlling demarcation liquid and enters woven hose 4; Second end of described woven hose 4 is connected with first end of a micron tube 7, and second end of this micron tube 7 is connected with first end of a transparent capillary 8, and second end of this transparent capillary 8 leads directly to atmosphere; One temperature conditioning unit 10 is electrically connected with power supply 20, and described micron tube 7 is in this temperature conditioning unit 10 inside, reaches the wherein temperature of liquid by temperature conditioning unit 10 control micron tube 7; One display module 11 is electrically connected with DATA REASONING and processing unit 9, is used to show measurement result; Described transparent capillary 8 is in described DATA REASONING and processing unit 9 inside, and this DATA REASONING and processing unit 9 are used for DATA REASONING and calculate blood viscosity, and result of calculation is shown by display module 11; One control module 13 is electrically connected with described valve 6, described DATA REASONING and processing unit 9, pressure source 1, is used for the work of control data measurement, processing and pressure source.
In the technique scheme, described temperature conditioning unit 10 is quoted the patent of the inventor in application on November 15th, 2002, and application number is 02148679.4, and name is called " method and the isolated plant that are suitable for temperature adjustment temperature control in the microscale experiment section "; Micron tube 7 of the present invention is placed in the experimental section runner of this temperature conditioning unit 10.
In the technique scheme, described DATA REASONING and processing unit 9 are made up of measurement module 21 and computing module 22; Comprise first photoelectric subassembly 23, second photoelectric subassembly 24 and timing circuit 25 in the measurement module 21, described first photoelectric subassembly 23 and second photoelectric subassembly 24 all adopt an optical transmitting set and an optical receiver to be placed on described transparent capillary 8 both sides and close described transparent capillary 8 respectively, be used for detecting transparent capillary 8 liquid levels and arrive, and send the liquid level arriving signal to DATA REASONING and processing unit 9; In measuring process, blood can flow in micron tube 7 and transparent capillary 8 under the effect of pressure source; Described measurement module 21 is set to: when first group of photoelectric subassembly 23 detects blood end face in the transparent capillary 8 through out-of-date, timing circuit 25 picks up counting, the blood end face is through out-of-date in the kapillary when second group of photoelectric subassembly 24 detects, and timing circuit 25 stops timing and the time data that writes down is input in the computing module 22; Described computing module 22 is designed to calculate according to relative measurement method the viscosity coefficient of blood.
The relative measurement method that the present invention adopts is: the amount relevant with the geometric parameter of micron tube 7 in the surveying instrument is made as constant C, and the liquid viscosity coefficient is expressed as follows,
Constant C is expressed as:
The viscosity coefficient of μ express liquid wherein, Q represents the flow of liquid in the micron tube 7, Δ P represents the pressure differential at liquid two ends in the micron tube 7; The present invention passes through known viscosity coefficient μ
MarkDemarcation liquid under the effect of pressure source 1, flow through micron tube 7, in transparent capillary 8, measure and demarcate flow of liquid and cross the used time t of two groups of photoelectric subassemblys 23,24 by measurement module 21
Mark, and store this time data; When blood flow is crossed two groups of photoelectric subassemblys 23,24, measure used time t in the same way
SurveyThe viscosity coefficient μ of blood then
SurveyFor:
Wherein, Q
Mark, Q
SurveyBe respectively the flow of demarcating liquid and blood to be measured; L is the distance between two groups of photoelectric subassemblys 23,24, and D is the interior diameter of transparent capillary 8.
The present invention designs and adopts relative measurement method is in order to check the state of instrument, to the more important thing is the physical dimension error of eliminating micron tube 7; Because the woven hose of instrument of the present invention 4, micron tube 7 and transparent capillary 8 are disposable use, each mismachining tolerance of replacing pipe is not necessarily identical, and according to following Hagen-Poiseuille formula:
Flow
(wherein, d is the interior diameter of micron tube 7, and k is the length of micron tube 7)
As seen, flow is directly proportional with the biquadratic of pipe diameter d, and mismachining tolerance is the main error that influences measuring error, so the present invention adopts relative measurement method that this factor is eliminated.
In the technique scheme, described pressure source 1 adopts the micro pressure source.
In the technique scheme, described control module 13 comprises at least three buttons and the corresponding circuit of demarcating button, zero clearing button and test button.
In the technique scheme, described control module (13) also comprises a load module, is electrically connected with temperature conditioning unit (10), is used to set the working temperature of temperature conditioning unit.
The measuring instrument course of work with real-time human body blood viscosity provided by the invention is as follows:
Calibration process:
1. opening power 1, opens temperature conditioning unit 10, and design temperature is treated its steady operation.
2. control module 13 is opened the valve 6 of demarcating liquid container 5, makes demarcation liquid enter woven hose 4 and micron tube 7, shut-off valve 6.
3. press the demarcation key of control module 13, pressure source 1 is started working, and demarcates liquid and flows through micron tube 7 and transparent capillary 8 under pressure, and DATA REASONING and processing unit 9 are measured and demarcated flow of liquid and cross the used time t of two groups of photoelectric subassemblys 23,24
Mark, and store this time data, calibration process finishes; Quiet back break-off is all blown with the liquid in woven hose 4, micron tube 7 and the transparent capillary 8 in control module 13 controlled pressure sources 1.
Measuring process:
4. woven hose 4, micron tube 7 and transparent capillary 8 integral body are taken off, rely on the capillary action absorption blood sample to be measured of woven hose 4 and micron tube 7 with first end of woven hose 4;
5. woven hose 4, micron tube 7 and transparent capillary 8 integral installations that will comprise blood sample to be measured are to instrument;
6. press the zero clearing button of control module 13 data are measured and processing unit 9 zero clearings, press the measurement button of control module 13 then, this moment, pressure source 1 was started working;
7. blood sample is under the effect of pressure source 1, and micron tube of flowing through 7 and transparent capillary 8 are measured blood flow by DATA REASONING and processing unit 9 and crossed the used time t of two groups of photoelectric subassemblys 23,24
SurveyWith the calculating viscosity, mu
Survey, result of calculation is input to display module 11;
8. display module 11 shows the viscosity coefficient of blood sample.
Compared with prior art, the invention has the beneficial effects as follows: clear operation and reading error, accuracy of detection and automaticity height; Blood using amount is less, only can measure with about 20ul blood; Woven hose 4, micron tube 7 and transparent capillary 8 are disposable use, avoid infection etc.; Simple to operate, be easy to carry, be adapted at hospital clinical or average family and use the human body blood viscosity.
Description of drawings
Fig. 1 is a structured flowchart of the present invention;
Fig. 2 is DATA REASONING and processing unit structured flowchart;
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail:
Embodiment 1
With reference to figure 1, make a measuring instrument with real-time human body blood viscosity of the present invention, comprising: power supply 20, pressure source 1, threeway 2, woven hose 4, valve 6, the demarcation liquid container 5 of demarcating liquid is housed, micron tube 7, transparent capillary 8, temperature conditioning unit 10, DATA REASONING and processing unit 9, display module 11 and control module 13.
The portable micro pressure pump that pressure source 1 adopts market to sell is such as the micropump in the EW3110 of the Panasonic sphygmomanometer.
Demarcating liquid container 5 volumes is 2ml, and interior dress is demarcated liquid, and this liquid is 10
-5The BAS of mol concentration, its viscosity coefficient are 8.9 * 10 in the time of 25 degrees centigrade
-4PaS.
The internal diameter of transparent capillary 8 is D=1mm, and the uniformity coefficient of internal diameter is ± 1%, and its length is greater than the distance between two groups of photoelectric subassemblys 23,24 in the measurement module 21 in DATA REASONING and the processing unit 9.Two groups of photoelectric subassemblys 23,24 in the measurement module 21 all adopt a light emitting diode and a photodiode, be placed on transparent capillary 8 both sides, and the outer wall of close transparent capillary 8, the liquid end face arrives situation in the transparent capillary 8 in order to measure, as shown in Figure 2.When the end face of liquid arrives first pair of photoelectric subassembly 23, the liquid barrier light path, trigger pip triggering timing circuit 25 of these group photoelectric subassembly 23 outputs picks up counting, when the liquid end face arrives second photoelectric subassembly 24, the liquid barrier light path, trigger pip triggering timing circuit 25 of these group photoelectric subassembly 24 outputs stops timing.In the present embodiment, the distance along transparent capillary 8 directions between two groups of photoelectric subassemblys 23,24 is 3mm.
The temperature conditioning unit 10 use inventor applied on November 15th, 2002, application number is " being suitable for the method and the isolated plant of temperature adjustment temperature control in the microscale experiment section " of 02148679.4, this device can satisfy the requirement of the present invention for the temperature control device miniaturization, the control temperature of temperature conditioning unit is set to fix in this enforcement 25 degrees centigrade.
The interior diameter of micron tube 7 is d=200um, and uniformity coefficient is ± 5%, length 50mm; First end of micron tube 7 is communicated with woven hose 4 by fluid sealant, and second end is communicated with by the transparent capillary 8 of fluid sealant with metering usefulness; The hermetic unit at micron tube 7 and two ends is placed in the experimental section runner of temperature conditioning unit 10 fully, makes that the liquid in the micron tube 7 flows through micron tube 7 under temperature constant state.
The LCM12232B that display unit 11 adopts Beijing high official position Creative Company to produce, operating voltage 5V, maximum operating currenbt 1mA is not with character library, and the master controller chip is the graphical dots configuration Liquid Crystal Module of SED1520.
The course of work of this embodiment is as follows:
Calibration process:
1. opening power 20, open temperature conditioning unit 10, treat its steady operation.
2. the valve of control module 13 unlatching demarcation liquid containers 5 was demarcated liquid and is entered woven hose 4 and micron tube 7 after 6,30 seconds, closed the valve 6 of demarcating liquid container 5.
3. press the demarcation key of control module 13, this moment, pressure source 1 was started working, and demarcated liquid and flow through micron tube 7 and transparent capillary 8 under pressure; 9 pairs of flows of DATA REASONING and processing unit are measured, process is as follows: the liquid end face successively passes through two groups of photoelectric subassemblys 23,24 of measurement module 21, and timing circuit 25 is noted the liquid end face and moved through the used time t of distance between two groups of photoelectric subassemblys 23,24
Mark, and store this data;
4. pressure source 1 worked on 30 seconds, and the residual demarcation liquid in woven hose 4, micron tube 7 and the transparent capillary 8 is all blown out from second end of transparent capillary 8, and calibration process finishes, pressure source 1 break-off.
Measuring process:
5. woven hose 4, micron tube 7 and transparent capillary 8 integral body are taken off, rely on the capillary action absorption blood sample to be measured of woven hose 4 and micron tube 7 with first end of woven hose 4;
6. will comprise treat the side blood sample woven hose 4, micron tube 7 and transparent capillary 8 integral installations to instrument;
7. press the zero clearing button of control module 13 data are measured and processing unit 9 zero clearings, press the measurement button of control module 13 then, this moment, pressure source 1 was started working;
8. blood sample is under the effect of pressure source 1, and micron tube of flowing through 7 and transparent capillary 8 are measured blood flow through the used time t of two groups of photoelectric subassemblys 23,24 by DATA REASONING and processing unit 9
Survey, calculate the viscosity of blood according to following formula:
9. DATA REASONING and processing unit 9 are input to display module 11 to blood viscosity value, show viscosity number.
With reference to shown in Figure 1, the woven hose 4 among the embodiment 1, micron tube 7 and transparent capillary 8 can also be made the pipeline of an integral body, and wherein the internal diameter parameter of each section is with embodiment 1, and the benefit of making an integral tube like this is to be convenient to change.
Embodiment 3
With reference to shown in Figure 1, on the basis of embodiment 1 or embodiment 2, control module 13 also comprises a load module (not shown), is connected with temperature conditioning unit 10, is used for setting the control temperature; According to environment temperature, can be by this module input one Temperature numerical near environment temperature, set the working temperature of temperature conditioning unit 10, like this can be so that temperature conditioning unit 10 reach the working temperature of setting soon under different environment temperatures, and steady operation.The span that the load module of present embodiment can be set the control temperature is 18 degrees centigrade, 20 degrees centigrade, 22 degrees centigrade, 24 degrees centigrade, 26 degrees centigrade, 28 degrees centigrade, 30 degrees centigrade, 32 degrees centigrade; DATA REASONING and processing unit 9 are designed to: the viscosity number tabulation of liquid under above-mentioned different temperatures demarcated in storage in advance, when setting the working temperature of temperature conditioning unit 10, DATA REASONING and processing unit 9 can be used for the calculating of blood viscosity according to the viscosity number of the temperature of setting extraction relevant temperature from the demarcation liquid viscosity value list of storage.
Claims (5)
1. the measuring instrument of a real-time human body blood viscosity, it is characterized in that, comprise: a power supply (20) is connected with a pressure source (1), pressure source (1) is connected by first end of a threeway (2) with a woven hose (4), one is equipped with between the demarcation liquid container (5) of demarcating liquid and this threeway (2) and is connected by a valve (6), and this valve (6) is used for controlling demarcation liquid and enters woven hose (4); Second end of described woven hose (4) is connected with first end of a micron tube (7), and second end of this micron tube (7) is connected with first end of a transparent capillary (8), and second end of this transparent capillary (8) leads directly to atmosphere; One temperature conditioning unit (10) is electrically connected with power supply (20), and described micron tube (7) is in the inside of this temperature conditioning unit (10), reaches the wherein temperature of liquid by temperature conditioning unit (10) control micron tube (7); One display module (11) is electrically connected with DATA REASONING and processing unit (9); Described transparent capillary (8) is in described DATA REASONING and processing unit (9) inside, is used for DATA REASONING and calculates blood viscosity, and result of calculation is shown by display module (11); One control module (13) is electrically connected with described valve (6), described DATA REASONING and processing unit (9), pressure source (1);
Described DATA REASONING and processing unit (9) are made up of a measurement module (21) and a computing module (22); Comprise first photoelectric subassembly (23), second photoelectric subassembly (24), timing circuit (25) and described transparent capillary (8) in the wherein said measurement module (21); Described first photoelectric subassembly (23) and second photoelectric subassembly (24) adopt an optical transmitting set and an optical receiver to be placed on described transparent capillary (8) both sides and close described transparent capillary (8) respectively.
2. the measuring instrument of real-time human body blood viscosity according to claim 1, it is characterized in that, described measurement module (21) is set to: when first group of photoelectric subassembly (23) detects liquid end face in the transparent capillary (8) through out-of-date, timing circuit (25) picks up counting, the liquid end face is through out-of-date in the kapillary when second group of photoelectric subassembly (24) detects, and timing circuit (25) stops timing and the time data that writes down is input in the described computing module (22).
3. the measuring instrument of real-time human body blood viscosity according to claim 1 is characterized in that, described control module (13) comprises that demarcating button, zero clearing button and at least three buttons and the corresponding custom circuit of test button is connected.
4. the measuring instrument of real-time human body blood viscosity according to claim 1 is characterized in that, described control module (13) comprises a load module, and this load module is electrically connected with temperature conditioning unit (10), is used to set the working temperature of temperature conditioning unit (10).
5. the measuring instrument of real-time human body blood viscosity according to claim 1 is characterized in that, described optical transmitting set is a light emitting diode; Described optical receiver is a photodiode.
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| CNB2005100642061A CN100549695C (en) | 2004-04-13 | 2005-04-12 | A kind of measuring instrument of real-time human body blood viscosity |
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Cited By (1)
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| TWI447375B (en) * | 2010-07-26 | 2014-08-01 | 私立中原大學 | Apparatus for measuring fluid viscosity and method thereof |
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| US8307697B2 (en) * | 2010-04-14 | 2012-11-13 | Ortho-Clinical Diagnostics, Inc. | Method for estimating viscosity |
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| CN108742562B (en) * | 2018-06-20 | 2021-04-20 | 博动医学影像科技（上海）有限公司 | Method and device for acquiring blood vessel pressure difference based on hyperlipidemia information |
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