CN100542544C - The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof - Google Patents
The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof Download PDFInfo
- Publication number
- CN100542544C CN100542544C CNB2007100200277A CN200710020027A CN100542544C CN 100542544 C CN100542544 C CN 100542544C CN B2007100200277 A CNB2007100200277 A CN B2007100200277A CN 200710020027 A CN200710020027 A CN 200710020027A CN 100542544 C CN100542544 C CN 100542544C
- Authority
- CN
- China
- Prior art keywords
- stomach
- gastric
- hours
- extract
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to a kind of mammiferous stomach extract, is raw material with the stomach or the mucosa of monogastric mammal, rubs, and adds pure water or normal saline, adds acidic materials and antiseptic and regulates serosity to pH 2～5, and 20～50 ℃ are stirred down and extracted 2～6 hours; Add edible alkaline material and be adjusted to pH6～7, cool to 8～12 ℃ gradually under continuing to stir, cross leaching filtrate; Filtrate decompression concentrates, and concentrate is put in the lyophilization machine enclosure, is solid through lyophilizing in 20～36 hours; Pulverize, be screened into powder and promptly get product.The enrichment of this extract the contained many biological activities of simple stomach type mammal gastric mucosa, these active ingredients have fabulous " homology " and " biocompatibility " with human stomach, can be used for adjusting the stomach function and treat multiple gastric disease.
Description
Technical field
The present invention relates to a kind of mammiferous stomach extract, the particularly simple stomach type mammal stomach extract of (promptly getting rid of the mammal contain a plurality of stomaches), and this extract is as the purposes in the gastrotherapy medicine.
Background technology
China has 200,000,000 people to suffer from chronic gastric disease approximately, at present commonly used in, not following tens kinds of western stomach medicine.Many " old gastropathy " take medicine countless in decades, still constantly have an attack of one's old illness.Study carefully its because of, this " gastropathy " and " stomach medicine " are interdependent for a long time, the origin of the difficult situation of " peaceful coexistence " is numerous stomach medicines " to the ill not to diseases ", due to " curing the symptoms, not the disease ".And treatment back relapse rate height, it is very fast to get damp again.Clinically even the just case report of recurrence of drug withdrawal three days arranged.
Atrophic gastritis (CAG) and gastric ulcer (GU) are common comparatively serious gastric disease.Their sickness rate is all very high, and different is their pathogenesis and symptom have very big difference even opposite.As CAG is uncomfortable after meal, achlorhydria; And GU is a pain and hyperchlorhydria ante cibum.Therefore medicine for treatment often has difference even opposite.But the patient who is no lack of chronic gastritis and peptic ulcer clinically and deposits makes the doctor in charge quite feel thorny.
The peptic ulcer agglutination is slow, is a kind of chronic and refractory ulcer, the pathogenesis complexity, the patient is subjected to ailing the torment for a long time, general health is on the weak side, so to the requirement of stomach medicine also than higher.Ideal stomach medicine wishes it is many components, the many target drugs that benefit is mended property, has the two-ways regulation function, easily is patient's acceptance, good effect and the minimum medicine of side effect.
Summary of the invention
The purpose of this invention is to provide a kind of mammiferous stomach extract and this extract medicinal usage as treatment gastropathy.
The mammiferous stomach extract of the present invention is made by following method:
(1) stomach or the mucosa with monogastric mammal (promptly getting rid of as many stomaches mammals such as cattle, camel, sheep) is raw material, after the raw material rubbing, the pure water or the normal saline that add 0.6～1 times of raw material weight, acidic materials and antiseptic with hydrochloric acid or pharmaceutically permission, regulate serosity and reach 2～5 (the best is 2.5～4) to pH, 20～50 ℃ are stirred extraction 2～6 hours (the best is 30～40 ℃ and stirred 3～5 hours down) down;
(2) slowly add edible alkaline material, adjust pH6～7, continue stirring and cool to 8～12 ℃ gradually down, filter
Get filtrate;
(3) filtrate decompression concentrates, and concentrate is put in the lyophilization machine enclosure, is solid through lyophilizing in 20～36 hours;
(4) solid is pulverized, is sieved to such an extent that powder promptly gets product (being called for short " defending stomach " down).
The available following method of above-mentioned steps (3) replaces: add organic solvent methanol, ethanol, acetone or isopropyl alcohol in the filtrate and make the generation precipitation, after aging, filter, with precipitate and place vacuum drying oven or ebullated dryer,＜40 ℃ got solid in dry 10～24 hours down, are ground into powder-product again.
Can add monogastric mammal other internal organs except that gallbladder, bladder, large intestine in the raw material of step (1), the ratio of interpolation is 0～1 times of whole stomach weight, perhaps 2.5～5 of gastric mucosa weight times.
Through protein detection (qualitative and quantitative), health examination can restrain or the 0.25 specification filling capsule that restrains by every 0.5 after the pharmacodynamics check, or make other oral formulations.
It is that raw material extracts that the present invention " defends stomach " and be with pig, Canis familiaris L., the stomach of monogastric mammal such as exempting from, and Main Ingredients and Appearance is a protein.Nonruminant is different with four stomach animals such as cattle, sheep, and the structure of their stomach and human stomach and function are very approaching.The present invention adopt first acidity, the back neutral extraction process, guaranteed that the extraction of bioactive substance is more complete, do not lose, enrichment that the extract of gained " is defended stomach " the contained many biological activities of these gastric mucosa of animal.These active ingredients have fabulous " homology " and " biocompatibility " with the mankind's stomach, can be used for adjusting stomach function and the multiple gastric disease of treatment.According to Biological Principles, select for use the growing animal stomach to make raw material, then contain higher biological activity in the extract of gained.
After testing, contain various active albumen, polypeptide, polysaccharide, enzyme and cell growth factor (as EGF), bifidus factor isoreactivity composition during the stomach extract of monogastric mammal of the present invention " is defended stomach ", have the medicinal usage of treatment gastric disease.
Show that through animal test results the present invention " defends stomach " and have the effect of following three aspects:
(1) recent effect: promote gastrointestinal peristalsis, improve digestive function; Increase appetite, the alcoholism that disappears, relieving constipation antidiarrheal; Be applicable to various anorexia, food stagnation, dyspepsia and baby's spitting milk etc.All-ages, without any side effects.
(2) mid-term effect: protection stomach, intestinal mucosa promote mucosal tissue new life.Can be used for chronic gastritis, chronic enteritis and chronic hepatitis or other functional gastrointestinal disorder that takes a disease and cause, repair and alleviate the injury of ethanol stomach, intestinal and liver.
(3) effect at a specified future date: contain abundant two qi factors, can promote a large amount of propagation of beneficial microbe " bifidus bacillus " in the intestinal, be dominant growth.The materials such as lactic acid that produced suppress assorted bacterium of enteral and pathogenic bacterium, and reduce the generation of organic amine, indole, nitrosamine materials such as (strong carcinogens), eliminate the chronic murder by poisoning of enterotoxin to human body, thus life lengthening.Two qi factors are regulated the very capable of intestinal microecology, the metabolite of bifidus bacillus not only can increase human body to trace element, absorption as calcium and ferrum, the immune disease-resistance ability of enhancing body also, suppress the growth of various cancerous cell (as mice S-180, Meth-A tumor cell), so have building body widely, the effect of anti-cancer and slow down aging.
The stomach extract of monogastric mammal of the present invention " is defended stomach " as gastric disease curative or stomach health care medication, dosage is for once-a-day, and each 0.6～1.5 gram (according to age, body weight and different) is taken before sleeping every night, can guarantee the abundant effect of medicine and coat of the stomach like this, the performance curative effect.Usually need every day and take medicine to compare for 2～3 times with existing stomach medicine, " defending stomach " taking convenience, curative effect is better.
From the mice pharmacological model, no matter be the gastric mucosa injury that causes by reserpine, hydrochloric acid, ethanol, " defending stomach " can both obviously suppress or alleviate it.Its effect is suitable with classical Western medicine " ranitidine ", it serves to show that " defending stomach " is at antiacid, anti-ethanol, protection gastric mucosa with promote that the function aspect its reparation is very remarkable.But there is bad side effect in ranitidine to human body, must strict control dose, and " defending stomach " action temperature and.From the rat pharmacological model, " defend stomach " to the secretion of the gastrointestinal hormone (as gastrin) of atrophic gastritis (CAG) or gastric ulcer (GU) rat model, has dual regulation, all can be to the stomach increased functionality, the direction that favourable reparation and minimizing are cancerated develops, and is a kind of medicine of many target spots, has the two-ways regulation effect, both can be used for CAG, also can be used for GU, in addition the present invention's the test of pesticide effectiveness also confirmed " defending stomach " but gastric injury that alleviation of alcohol causes.
In sum, " defend stomach " and derive from stomach, it is the natural drug of treatment chronic gastritis, gastric ulcer, alcoholic gastritis, it is the therapeutic agent of comparatively ideal multiple gastric disease, can releive or eliminate the painful of vast patients with gastric disease to greatest extent and improve its prognosis, avoid the further deterioration canceration of atrophic gastritis (CAG) and gastric ulcer (GU), action temperature with, be free from side effects, demonstrated fully " food stomach tonify deficiency ", " with dirty foster dirty " this Traditional Chinese medical theory.
Description of drawings
Accompanying drawing represents that the ultraviolet light scanning that animal stomach extract of the present invention " is defended stomach " absorbs collection of illustrative plates.
The specific embodiment
It is that feedstock production " is defended stomach " that embodiment 1 usefulness Gaster Sus domestica mucosa adds Pancreas Sus domestica
Get 2 kilograms of 1 kilogram of Gaster Sus domestica mucosa and Pancreas Sus domesticas, rub respectively and make raw material.Add 2 liters in water in the container, drop into raw material in the stirring at twice and make homogenate.Transfer to pH4 with Sal, hydrochloric acid, under 35 ℃ of temperature, stir and extracted 5 hours.Adjust feed liquid to pH6～7 with edible base then, and under agitation be cooled to about 10 ℃ with psychrolusia.Filter cloth filters removes filtering residue, and 3 liters of filtrates of gained are concentrated into 1.8 liters with thin film concentrator at 30 ℃.Sabot, with freeze dryer lyophilizing 22 hours, the gained solid through pulverize, sieve 108 gram " defending stomach " powder (sample A).
Example 2 usefulness Gaster Sus domestica are that feedstock production " is defended stomach "
Get 1 kilogram in piglet stomach, rub and add 0.6 liter in water in the rearmounted reactor, Sal and acetic acid a little, slip is transferred to pH3.5,38 ℃ are stirred down and extract after 4 hours, adjust feed liquid to pH6-7 with edible base.Continue stirring reuse ice bath or water-bath and be cooled to 12 ℃.Centrifugal filtration gets 1.1 liters of filtrates; Filtrate adds 4 liters of edible ethanols under stirring, and leaves standstill, and treats centrifugal collecting precipitation after the layering.After dewatering, pressing dry, moved into vacuum drying oven dry 10 hours, and got drying solid, pulverize, sieve to such an extent that " defending stomach " pale powder 28 restrains (sample B) again.
More than " defending stomach " sample A and sample B scan qualitative detection through ultraviolet light, the gained collection of illustrative plates is as shown in drawings.Visible maximal ultraviolet photoscanning absorption collection of illustrative plates shows its maximum absorption band at 257.7 ± 2.5nm place among the figure, and pointing out its Main Ingredients and Appearance is protein and peptide matters.
More than " defending stomach " sample A and sample B as follows through UV Absorption (quantitatively) test result:
Embodiment 3: " defending stomach " is to the therapeutic effect test of mice reserpine type gastric ulcer
60 of mices are divided into following four groups, 1.～3. organize 10 every group, and the 4. totally 30 of the groups are divided into three groups again, 10 of every groups.Wherein 2.～4. every mice of each group is irritated stomach (by 10mg/kg dosage) with reserpine and makes gastric injury cause the gastric ulcer modeling.
1. blank is organized (10 mices): give the equivalent distilled water and irritate stomach.
2. matched group (10 mices): give the equivalent distilled water and irritate stomach.
3. ranitidine group (10 mices): give ranitidine 20mg/kg and irritate stomach.
4. the group (30 mices) of " defending stomach ": per 10 one groups, three groups give 0.3,0.1 respectively, " defending stomach " sample A of 0.03g/kg various dose irritates stomach.
After the administration hour, put to death mice, get stomach, inject the formaldehyde fixed of 2mL 1%.
The gastric mucosa injury index of mice is respectively organized in test, and the test assessment method is as follows: not damaged mucosa 0 minute; Point-like damage 〉=1mm, 1 o'clock is 0.2 minute, 2 o'clock is 0.4 minute; 1 of strip damage is 1 minute, and 2 is 2 minutes, and the like; Gastric mucosa is completely without being 5 minutes; The part mucosa is arranged, look serious situation and be decided to be 4,3,2 fens.Evaluation result such as table 1.
Subordinate list 1 " is defended stomach ", ranitidine compares the action effect of mice reserpine type gastric ulcer
Embodiment 4 " defends stomach " to the therapeutic effect test of mice salt acid type gastric ulcer
1. the method identical with embodiment 3 be divided into 60 mices～and 4. four groups, mice of 2.～4. each group that different is is irritated stomach (0.10ml/) with 0.6mol/L hydrochloric acid makes gastric injury cause gastric ulcer.All the other processs of the test are identical with embodiment 3.Use the gastric mucosa injury index of respectively organizing mice with the quadrat method test then, result such as table 2.
Subordinate list 2 " is defended stomach ", ranitidine compares the action effect of mice salt acid type gastric ulcer
The generation of reserpine type ulcer increases relevant with vagal excitation and gastric acid secretion.From the test result (table 1) of embodiment 3 as seen, " defending stomach " can obviously dwindle the ulcer area that reserpine causes, illustrates that it has the effect that promotes ulcer healing.The test result (table 2) of embodiment 4 shows, " defending stomach " can not only prevent because gastric acid secretion increases the gastric ulcer (table 1) that (reserpine type) causes, can also be to adding the inhibitory action that plays of ulcer due to the acid stimulus object (hydrochloric acid).
Embodiment 5 " defends stomach " to the therapeutic effect test of mice dehydrated alcohol type gastric ulcer
1. mice is divided into～4. group by last two embodiment methods, wherein 3.～4. every mice of each group is with the modeling of 0.15ml dehydrated alcohol and measure damage index. and 1. blank group reaches matched group and 2. gives the equivalent distilled water, and it is routine constant by last two that 3. other organize and 4. organize dosage.With the gastric mucosa injury index of respectively organizing mice with the quadrat method test, result such as table 3.
Table 3 " is defended stomach " and the action effect of ranitidine to mice dehydrated alcohol type gastric ulcer
Embodiment 6
60 mices are divided into 6 groups, and 10 every group, wherein 1 group is matched group, gives distilled water, and all the other 5 groups are " defending stomach " group, give " defending stomach " 0.3g/kg and irritate stomach.The group mice of " defending stomach " is in after the administration 0.5,1,2,3, irritate stomach with the 0.15ml dehydrated alcohol behind the 4h.0.5 the same method test gastric mucosa injury index (being the ulcer scoring) sees Table 4 after hour.
Table 4 " is defended stomach " to the lasting inhibitory action of mice dehydrated alcohol type gastric ulcer
Table 4 shows, the gastric mucosal protection effect with " defending stomach " administration after time length and difference, ulcer inhibition rate is the highest behind the administration 0.5h, but behind administration 3h, still keeps certain effect.As seen " defend stomach " keeping the mucosa protective effect time is 3h, greater than the time of general medicine through the mice gastric emptying.
At present still be in developmental stage for the cause of disease of gastric ulcer, the understanding of pathogenesis.The pathomechanism of generally acknowledging is like attack factor and defense factor dysequilibrium theory, promptly multifactor pathogenesis.Experimental selection of the present invention reserpine, hydrochloric acid, dehydrated alcohol induced mice gastric ulcer model, reflected directly that the present invention " defends stomach " antiacid and increase the ability of mucosa protective effect.
Confirm by a plurality of experimental ulcer scale-model investigations, " defend stomach " and can not only prevent by gastric acid secretion and increase (reserpine type) and add the generation of ulcer due to the acid stimulus object (hydrochloric acid), can also play inhibitory action to the formation of ulcer (ulcer due to the dehydrated alcohol) under the non-gastric acid secretion incentive condition, the effect comparison is better according to the medicine ranitidine.Preliminary pharmacodynamic experiment ulcer model has comprised that the gastric mucosa attack factor strengthens and defense factor weakens two aspects.Contrast " defending stomach " all presents tangible drug effect, illustrates that this medicine is a kind of medicament for resisting peptic ulcer of many target spots preferably.
Example 7 " is defended stomach " to the therapeutic effect of experimental rat chronic atrophic gastritis (CAG)
The intravital motilin of people all has intense influence to gastrointestinal motion and electrical activity, can stimulate pepsic secretion, and it shrinks the esophagus hypomere by bringing out duodenum generation interdigestive myoelectric complex III phase, and the stomach motion increases, but the emptying of the stomach that slows down.In addition, the main effect of the gastrin of human body is a gastric acid secretion, also can promote the division growth of cell simultaneously, and DNA and RNA synthetic ratio are increased, and mucosal blood flow increases, stomach and duodenal mucosa plumpness, and the parietal cell number increases.So the gastrin secretion reduces and even lacks, and can cause the gastric mucosa malnutrition, or increase the weight of the atrophy of body of gland.And the gastrin level is too high, makes the gastric acid excessive secretion, can destroy the gastric mucosal protection barrier, causes the ulcer or the ulcer healing speed that slows down.
Present embodiment with in four groups of rats three groups irritate the stomach modeling with 2% sodium salicylate aqueous solution respectively, the 9th week rose, group gives " defending stomach " sample B (0.03g/kg) filling stomach " to defend stomach ", and the positive drug group gives bismuth potassium citrate 2ml/ and only irritates stomach, and blank group and matched group give the equivalent distilled water and irritate stomach.After 12 weeks, put to death, get blood, adopt radio immunoassay (RIA method) to detect motilin (MTL), gastrin (GAS).Result such as table 5.
Table 5 " is defended stomach " to the influence (pg/ml) of CAG rat plasma MTL level
*: compare P＜0.05 with the blank group; ##: compare P＜0.05 with natural recovering group.
Table 6 " is defended stomach " to the influence (Pg/ml) of CAG rat blood serum Gas level
*: compare P＜0.05 with the blank group; ##: compare P＜0.05 with natural recovering group.
Table 5 result shows that " defending stomach " can significantly reduce plasma motilin, improves level of serum gastrin, and effect is better than the positive drug bismuth potassium citrate." defending stomach " can accelerate the emptying of stomach by reducing the plasma motilin of CAG rat model, reduces the stop at gastric of food and gastric juice, promotes digesting and assimilating of food, avoids gastric acid to cause ulcer at the gastric overstand.Table 6 shows " defending stomach " can raise level of serum gastrin of CAG rat, promotes the secretion of gastric acid, promotes the synthetic of DNA and RNA, increases mucosal blood flow, brings into play its Nutrition to gastric mucosa, promotes to repair.
Claims (6)
1. the stomach extract of animal is characterized in that being made by following method:
(1) stomach or the gastric mucosa with monogastric mammal is raw material, after the raw material rubbing, add the pure water or the normal saline of 0.6～1 times of raw material weight, the acidic materials and the antiseptic that add hydrochloric acid or pharmaceutically allow, regulate serosity and reach 2～5 to pH, 20～50 ℃ are stirred extraction 2～6 hours down;
(2) slowly add edible alkaline material and be adjusted into pH6～7, cool to 8～12 ℃ gradually under continuing to stir, cross leaching filtrate;
(3) filtrate decompression concentrates, and concentrate is put in the lyophilization machine enclosure, is solid through lyophilizing in 20～36 hours;
(4) solid is pulverized, is sieved to such an extent that powder is the stomach extract product of monogastric mammal.
2. animal stomach extract according to claim 1 is characterized in that the pH in the said step (1) is 2.5～4
3. mammiferous stomach extract according to claim 1 is characterized in that the extraction temperature in the said step (1) is 30～40 ℃, stirs 3～5 hours.
4. animal stomach extract according to claim 1, it is characterized in that step (3) replaces in order to following method: add organic solvent methanol, ethanol, acetone or isopropyl alcohol in the filtrate and make the generation precipitation, after aging, filter, got the pressed powder product down in dry 10～24 hours with precipitate and at＜40 ℃.
5. according to claim 1 or 2 or 3 or 4 described animal stomach extracts, it is characterized in that adding in the raw material of step (1) monogastric mammal other internal organs except that gallbladder, bladder, large intestine, the ratio of adding is 0～1 times of whole stomach weight, perhaps 2.5～5 of gastric mucosa weight times.
6. the application of the animal stomach extract of claim 1 in the medicine of preparation treatment atrophic gastritis, gastric ulcer or alcoholic gastritis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100200277A CN100542544C (en) | 2007-02-07 | 2007-02-07 | The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100200277A CN100542544C (en) | 2007-02-07 | 2007-02-07 | The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101011416A CN101011416A (en) | 2007-08-08 |
| CN100542544C true CN100542544C (en) | 2009-09-23 |
Family
ID=38699251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100200277A Expired - Fee Related CN100542544C (en) | 2007-02-07 | 2007-02-07 | The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100542544C (en) |
-
2007
- 2007-02-07 CN CNB2007100200277A patent/CN100542544C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101011416A (en) | 2007-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103652361B (en) | Feed for postpartum sow in lactation period of 1-20 days and preparation method thereof | |
| CN103689296B (en) | Feed for ewe at later pregnancy and lactation period and preparation method thereof | |
| CN103608025B (en) | For preventing or treat the compositions comprising herb extracts of neurodegenerative diseases | |
| CN102754754A (en) | Whole oat type piglet compound feed and preparation method thereof | |
| CN102362627A (en) | Healthcare coffee containing cordyceps | |
| CN103652360A (en) | Feed for curing pig paratyphus and preparation method thereof | |
| JP4132635B2 (en) | Uninactivated enzyme-enhanced composition | |
| CN101861903A (en) | Coffee with weight reducing and antifatigue functions | |
| CN104256165A (en) | Functional feed for preventing and treating chronic swine plague and preparation method of functional feed | |
| JP3691685B2 (en) | Blood sugar level rise inhibitor | |
| CN109699890A (en) | A kind of herbal formulation that can alleviate gout and high lithemia | |
| CN102973829A (en) | Feed for treating diarrhea of dairy cow | |
| CN113413454A (en) | Composition for promoting gastric mucosa injury repair and preparation method thereof | |
| CN102776102B (en) | Health wine containing bee products | |
| CN103719997B (en) | A kind of guava solid beverage | |
| CN110075280A (en) | A kind of compound oral agents of laminin peptide and preparation method thereof | |
| CN100542544C (en) | The medicinal usage of animal stomach extract and conduct treatment gastropathy thereof | |
| CN100506085C (en) | Brown sugar health product capable of raising immunity of puerpera | |
| CN1205944C (en) | Composition of Chinese medicine | |
| CN106490268A (en) | One kind is for deinebriating tea-drinking and preparation method thereof | |
| JP2001103933A (en) | Mixed food and feed additive each containing spore of bracket fungus of genus fomes | |
| TWI517853B (en) | A composition for treating a vascular injury or a disease-related disease | |
| CN104042879B (en) | Traditional Chinese medicine granules for reversing precancerous lesions of chronic atrophic gastritis and preparation method thereof | |
| CN111643641B (en) | Food therapy formula for clearing heat, invigorating spleen and tonifying kidney and preparation method and application thereof | |
| CN104825379A (en) | Whitening peeling night cream capable of reducing melanin deposition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090923 Termination date: 20150207 |
|
| EXPY | Termination of patent right or utility model |