CN100526472C - Calf serum 'double resistant' culture medium - Google Patents
Calf serum 'double resistant' culture medium Download PDFInfo
- Publication number
- CN100526472C CN100526472C CNB2007100193502A CN200710019350A CN100526472C CN 100526472 C CN100526472 C CN 100526472C CN B2007100193502 A CNB2007100193502 A CN B2007100193502A CN 200710019350 A CN200710019350 A CN 200710019350A CN 100526472 C CN100526472 C CN 100526472C
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- Prior art keywords
- calf serum
- culture medium
- bacterium
- double resistant
- milliliters
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Abstract
The invention discloses a liquid culture medium, which is allocated by calf serum, glucose peptone water, vancocin and polymyxin B to prevent non-target bacterial fertility.
Description
Technical field
The present invention relates to the substratum of " Micro biological Tests " aspect in a kind of being applied to " medical microbiology " " pathogenic bacteria (Neisseria meningitidis) " check.
Background technology
Neisseria meningitidis is very high to the requirement of environmental factors, after leaving human body, very easily deadly when environment is not suitable for (be lower than 22 ℃ as temperature, dead in 2 hours), but the epidemic season of the popular meninges that is caused by Neisseria meningitidis is a winter-spring season, thereby the winter-spring season in cold is had in the investigation work of carrying disease germs.Before this technological invention, the check of Neisseria meningitidis is a throat swab of gathering patient, patient suspected or carrier, with throat swab direct inoculation blood agar plate or chocolate blood agar plate substratum.Also having seldom, some people inoculates above-mentioned substratum after with " the two anti-salt solution of yolk " sample being transported to the laboratory earlier.There are some shortcomings in these substratum: the one, and the preparation of " yolk two anti-salt solution " is very loaded down with trivial details, needs 75% alcohol-pickled sterilization egg, removes egg white, collects yolk, operation such as smashes, and the substratum of making can't be sterilized, and can not guarantee aseptic; The 2nd, to transport a large amount of plate culture mediums during to spot sampling and need heat-preserving equipment, glass dish not only can damage in the transportation, and can cause the pollution to sample, and is concerning work, very inconvenient; The 3rd, above-mentioned substratum must be cultivated behind seed sample immediately, has cultivated with regard to inconvenience when the laboratory is far away far from the sampling location, and at this moment the bacterium in the sample will be dead gradually, and detect the possibility of purpose bacterium after having reduced; The 4th, above-mentioned plate culture medium is a solid medium, only produces single bacterium colony during their culturing bacterium, do not have the bacterium of increasing effect, so bacteria containing amount more just can't detect in the sample.
Summary of the invention
For overcoming the deficiencies in the prior art part, the object of the present invention is to provide a kind of make simple, easy to use, easily obtain result's novel culture medium.
For achieving the above object, the technical scheme that the present invention takes is: the albumen of utilization in the calf serum prevents the death of purpose bacterium and a large amount of breedings that nutrition dual function, vancomycin and PXB prevent non-purpose bacterium, makes liquid nutrient medium into, make it have the bacterium of guarantor, increase the bacterium dual function, before cultivation, increase bacterium earlier, carry out separation and Culture again after increasing bacterium.Through experimental study, we have determined that the volume percent of calf serum ' double resistant ' culture medium prescription and each composition is: calf serum 50%, 1% glucose and 1% peptone water 49.5%, every milliliter 660 unit vancomycin and 5000 unit polymyxin solution 0.5%.
Because this substratum has the effect of protecting bacterium, increasing bacterium simultaneously, obtained some beneficial effects: the one, can guarantee that in the time of 4 ℃ A and C group meningitis Neisseria are not dead in 96 hours, the 2nd, when remote sampling, can avoid the death of purpose bacterium, before formal check, make the amplification in advance of purpose bacterium, increased the bacteria containing amount of purpose bacterium in the sample, can improve the recall rate of purpose bacterium, pairing to 310 parts of effective samples detects, and detects positive rate and rises to 1.94% by 0%.Solved transportation problem after away from breadboard spot sampling, can prevent the death of purpose bacterium, the breakage of the equipment that prevents to sample, when the purpose bacterial content is very low in sample, can improve the purpose bacterium recall rate, reduced the labour intensity when great amount of samples is checked.
Embodiment
Preparation glucose peptone water solution: take by weighing glucose 2 grams, peptone 2g is dissolved in 200 ml distilled waters, 121 ℃ of sterilizations in 15 minutes, and the cooling back is standby.
Preparation " two anti-" aqueous solution: take by weighing pulvis vancomycin 0.0948 and restrain in the 100ml saline bottle of the sterilization of packing into, add 9.48 milliliters of sterile purified waters; Taking by weighing pulvis AEROSPORIN 0.0931 restrains in the 100ml saline bottle of the sterilization of packing into, add 9.31 milliliters of sterile purified waters, after it is fully dissolved, draw 8.33 milliliters of 6.6 milliliters of vancomycin solution, AEROSPORIN solution respectively in another sterile saline bottle, replenish sterile purified water to 100 milliliter, make the final concentration of vancomycin, AEROSPORIN be respectively 660U/ml and 5000U/ml.
Purchase the some milliliters of aseptic commodity calf serum.
Preparation calf serum ' double resistant ' culture medium: 198 milliliters of glucose peptone water solution, " two anti-" 2 milliliters of aqueous solution, 200 milliliters of the aseptic commodity calf serums of getting above-mentioned preparation, fully behind the mixing, be sub-packed in the aseptic test tube with 10 milliliters of aseptic suction pipes, 1 milliliter of every pipe, filled in can take spot sampling behind the test tube to and cultivate before increase bacterium.
Claims (1)
1. calf serum ' double resistant ' culture medium, described substratum is a kind of liquid nutrient medium, and the volume percent of its prescription and each composition is: calf serum 50%, 1% glucose and 1% peptone water 49.5%, every milliliter 660 unit vancomycin and 5000 unit polymyxin solution 0.5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100193502A CN100526472C (en) | 2007-01-17 | 2007-01-17 | Calf serum 'double resistant' culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100193502A CN100526472C (en) | 2007-01-17 | 2007-01-17 | Calf serum 'double resistant' culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101012476A CN101012476A (en) | 2007-08-08 |
| CN100526472C true CN100526472C (en) | 2009-08-12 |
Family
ID=38700204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100193502A Expired - Fee Related CN100526472C (en) | 2007-01-17 | 2007-01-17 | Calf serum 'double resistant' culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100526472C (en) |
-
2007
- 2007-01-17 CN CNB2007100193502A patent/CN100526472C/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 自制解脲与人型支原体培养基的实验研究. 陈芳等.中国微生态学杂志，第16卷第6期. 2004 |
| 自制解脲与人型支原体培养基的实验研究. 陈芳等.中国微生态学杂志，第16卷第6期. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101012476A (en) | 2007-08-08 |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090812 Termination date: 20120117 |