CN107446986A - Culture medium of microorganism and preparation method thereof in a kind of detection blood of human body - Google Patents
Culture medium of microorganism and preparation method thereof in a kind of detection blood of human body Download PDFInfo
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- CN107446986A CN107446986A CN201710605692.6A CN201710605692A CN107446986A CN 107446986 A CN107446986 A CN 107446986A CN 201710605692 A CN201710605692 A CN 201710605692A CN 107446986 A CN107446986 A CN 107446986A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Abstract
The present invention relates to technical field of biological, more particularly to culture medium of microorganism and preparation method thereof, the culture medium for detecting microorganism in blood of human body, including basal medium and additive in a kind of detection blood of human body, in terms of mass fraction, the additive includes following material：5 15 parts of peptone, 2.5 7.5 parts of sodium chloride, 13 parts of glucose, 0.5~4 part of lactose, 1.5 3.5 parts of phosphorus source, 0.01~0.08 part of Pig cholate, 0.5~6 part of mannitol, 13 parts of Sodium Pyruvate, amino acid/11~6 part, 1~8 part of β ribosides zymolyte, 1~3 part of mango extract solution, 0.02~0.58 part of metal salt, 0.01~0.05 part of surfactant, pH value is 7.2 7.5, 1000 parts of distilled water, the real content for being directly inferred to different microorganisms in blood can be counted by bacterium colony with the solid medium of the present invention, being accurately judged to blood from quantitative angle is in carrier state or has reached pathogenic concentration, so that the accuracy of testing result is further enhanced.
Description
Technical field
The present invention relates to technical field of biological, more particularly to it is a kind of detect in blood of human body the culture medium of microorganism and
Its preparation method.
Background technology
The blood of human normal be do not have it is germy.After human body is by bacterium infection, according to by bacterium infection degree
Difference, blood simply can be divided into bacteremia, septicemia, septicopyemia etc. by illness caused by bacterium infection.Resisting
Bacterium infection human body typically first causes certain a part of inflammation, such as furuncle, carbuncle, pneumonia, encephalitis in the case that power reduces.Medically
Because bacterium by local inflammation position enter blood circulation illness call bacteremia.At this moment a small amount of bacterium intrusion blood is often had
Liquid, if the systemic conditions of people are fine, this small amount of bacterium can be by caused various antibody, phagocyte and blood in body
Leucocyte eliminate.And if angioinvasive germ is a lot, toxicity is very strong, or due to human body overwork, malnutritive, serious
Wound or have the reasons such as other diseases and during extremely low resistance, bacterium can amount reproduction simultaneously produce poisonous and harmful substance, with
Flow propagation is to whole body.At this moment patient shows as the serious poisoning symptom such as high fever, shiver with cold, headache, and body temperature is often up to more than 40 DEG C.
Meanwhile bacterium can invade each internal organs and cause the inflammation of internal organs, suppurate.If sending out skin, various fash are may occur in which.Medical science
On bacterium enter blood after in blood illness caused by growth and breeding call septicemia.If suppurative bacterium enters blood
After liquid, not only breed in blood, cause some internal organs to form abscess even organ dysfunction, perfused tissue bad or low
Blood pressure, this illness are then referred to as septicopyemia.Pyemia case fatality rate is higher, and purulence can occur for about 9% sepsis patient
Toxicogenic shock and more device pipe insufficiencies, the death of more than half is by septic shock and multiple organ dysfunction in intensive care unit
Caused by incomplete.Research shows, occur internal organs organ failure, shock, multiple infection, serious potential disease patient's prognosis compared with
Difference.Above bacteremia, septicemia, a common ground of septicopyemia these three illnesss are that have bacterium in blood samples of patients.If
Cultivated with blood samples of patients, you can to detect pathogenic bacteria.Therefore, diagnosis of the result to disease of blood Bacteria Culture and identification with
Treat significant.
In general, the sample of blood Bacteria Culture is acquired, is incubated, culture transferring, is tentatively judged, then further according to each
The Identification of Biological Characteristics of kind pathogen sends report.But in actual clinical inspection, usually occur some specific pathogenic
Bacterium fails the situation of detection.Especially in recent years, in rising trend, many preferable hospital's bases of appointed condition occur for blood infection
This carries out blood Bacteria Culture using the equipment of automatic blood Bacteria Culture, and the positive rate that blood culture generally be present is inadequate
, easily there is the problems such as pollution causes error result in height, cultivation cycle length, and this is to selecting medication, timely and effectively in clinical treatment
Infection control and last Case treatment effect all have very tremendous influence.
The content of the invention
In view of the shortcomings of the prior art, the main object of the present invention is to provide a kind of training for detecting microorganism in blood of human body
Base and preparation method thereof is supported, he can promote microorganism to grow and be enriched with, and improve the concentration of microorganism, accurate so as to improve detection
Rate.
To solve the above problems, the invention provides a kind of culture medium for detecting microorganism in blood of human body, including basis
Culture medium and additive, in terms of mass fraction, the additive includes following material：Peptone 5-15 parts, sodium chloride 2.5-7.5
Part, glucose 1-3 parts, 0.5~4 part of lactose, phosphorus source 1.5-3.5 parts, 0.01~0.08 part of Pig cholate, 0.5~6 part of mannitol,
Sodium Pyruvate 1-3 parts, amino acid/11~6 part, β -1~8 part of riboside zymolyte, 1~3 part of mango extract solution, metal salt 0.02~
0.58 part, 0.01~0.05 part of surfactant, 1000 parts of distilled water.
Preferably, it is described detection blood of human body in microorganism culture medium, in terms of mass fraction, the additive include with
Lower material：6~12 parts of peptone, 3~5.8 parts of sodium chloride, 1.2~2.8 parts of glucose, 0.8~3.5 part of lactose, phosphorus source 1.8~
3.2 parts, 0.02~0.06 part of Pig cholate, 1.5~4.2 parts of mannitol, 1.5~2.2 parts of Sodium Pyruvate, amino acid/11 .9~4.3
Part, β -2~6 parts of riboside zymolyte, 1.2~2.3 parts of mango extract solution, 0.05~0.36 part of metal salt, surfactant
0.02~0.03 part, 1000 parts of distilled water.
Preferably, it is described detection blood of human body in microorganism culture medium, in terms of mass fraction, the additive include with
Lower material：10 parts of peptone, 4.8 parts of sodium chloride, 2.3 parts of glucose, 3.2 parts of lactose, 2.4 parts of phosphorus source, 0.05 part of Pig cholate are sweet
Reveal 3.6 parts of alcohol, Sodium Pyruvate 1-3 parts, 2.6 parts of amino acid, β -4 parts of riboside zymolyte, 1.8 parts of mango extract solution, metal salt
0.16 part, 0.02 part of surfactant, 1000 parts of distilled water.
The present invention does not have special requirement to the species of basal medium, can often know for those skilled in the art, example
Such as, the culture medium is selected from MEM culture mediums, DMEM culture mediums, DMEM-F12 culture mediums (1:1), HB culture mediums, BEM culture mediums
In one kind.
In the present invention, peptone provides nitrogen source and carbon source for the growth of bacterium, is an important factor for maintaining cell growth, originally
In invention, the peptone can be phytone or animal protein peptone, it is preferred that the peptone be soy peptone,
At least one of blood peptone, tryptone, meat peptone.
Phosphate can both provide enough P elements for microorganism, can also pH value in buffer culture medium, it is preferred that institute
Phosphorus source is stated as at least one of sodium dihydrogen phosphate, disodium hydrogen phosphate, dipotassium hydrogen phosphate and potassium dihydrogen phosphate.
Amino acid is one kind of growth factor, the growth factor amount very little required for microorganism growth, and microorganism sheet
Body can not synthesize or synthetic quantity deficiency, it is impossible to which required for meeting body growth, the growth of microorganism or the synthesis of enzyme need amino
Acid, but microorganism lacks the ability of synthesizing amino acid in itself, it is necessary to amino acid is added in the medium or contains amino acid
Polypeptide, the normal growth of bacterium could be maintained.Preferably, the amino acid is glycine, ALANINE, Pidolidone, L- paddy
Glutamine, L-arginine, L-Histidine, L-Leu, ILE, Serine, L-threonine, TYR, L- rely
In propylhomoserin, L-Methionine, L-phenylalanine, L-PROLINE, L-Trp, Valine, L-Aspartic acid and altheine
At least one.
Surfactant can change the permeability of cell, and so as to be advantageous in cell building-up process, the release of enzyme is excellent
Choosing, the surfactant is SDS and/or CTAB.
Metal salt be also maintain cell growth another key factor, it is preferred that the metal salt be molysite, mantoquita,
At least one of zinc salt, cobalt salt, manganese salt, chromic salts, selenium salt, nickel salt, molybdenum salt, vanadic salts, pink salt, strontium salt.
Preferably, the β-riboside zymolyte is 2- hydroxyphenyls-β-D-RIBOSE glycosides (catechol-β-D-ribose
Glycosides), magenta-β-D-ribose glycosides (the chloro- 3- indoxyls-β of the bromo- 6- of 5--D-ribose glycosides), dihydroxyflavone-β-D-ribose glycosides；X-
β-D-ribose glycosides (the chloro- 3- indoxyls-β of the bromo- 4- of 5--D-ribose glycosides), pink-β-D-ribose glycosides (the chloro- 3- indoxyls of 6--
β-D-ribose glycosides), the bromo- 3- indoxyls-β of 6--D-ribose glycosides, the bromo- 3- indoxyls-β of 5--D-ribose glycosides, the fluoro- 3- indoles of 6-
Epoxide-β-D-ribose glycosides；Alizarin-β-D-ribose glycosides, (P)-nitrobenzophenone-β-D-ribose glycosides, 4-methyl umbelliferone base-β-D- cores
At least one of glucosides, naphthols-β-D-ribose glycosides, dichloro aminophenyl-β-D-ribose glycosides.
The preparation method of the culture medium of microorganism, comprises the following steps in a kind of detection blood of human body：
(1) by peptone, sodium chloride, glucose, lactose, phosphorus source, Pig cholate, mannitol, Sodium Pyruvate, amino acid, β-
Glucoside, mango extract solution, metal salt, surfactant are well mixed in water, after being well mixed, regulation pH most 7.2~
7.5；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 1.~3 × 105Sterilized at Pa, 121 DEG C ± 1 DEG C of temperature
20-30 minutes, 45~60 DEG C are then naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Compared with prior art, the present invention has following technique effect：
Culture medium of the present invention provides beneficial conditions for the growth and breeding of microorganism, can promote procreation and the richness of microorganism
Collection, improve the concentration of microorganism.It can be counted with the solid medium of the present invention by bacterium colony and directly be inferred to difference in blood
The real content of microorganism, being accurately judged to blood from quantitative angle is in carrier state or has reached pathogenic dense
Degree so that the accuracy of testing result is further enhanced.The incubation time of microorganism is short in the present invention, at present solid culture
The result interpretation time of base is 24 hours, and only needs 10~15 hours using the culture medium sentence read result of the present invention, is contracted significantly
Short detection time, viral quick detection.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, following examples, the present invention is carried out
It is further described.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit
The present invention.
Embodiment 1
The culture medium of microorganism in a kind of detection blood of human body, including basal medium and additive, in terms of mass fraction,
The additive includes following material：
10 parts of soy peptone, 4.8 parts of sodium chloride, 2.3 parts of glucose, 3.2 parts of lactose, 2.4 parts of sodium dihydrogen phosphate, pig gall
0.05 part of salt, 3.6 parts of mannitol, 2 parts of Sodium Pyruvate, 2.6 parts of glycine, 2- hydroxyphenyls-β-D-RIBOSE glycosides (catechol-
β-D-ribose glycosides) 4 parts, 1.8 parts of mango extract solution, 0.16 part of molysite, 0.02 part of surfactant, pH value 7.3, distilled water
1000 parts；
The present embodiment additionally provides the preparation method of the culture medium of microorganism in the detection blood of human body, including following step
Suddenly：
(1) by soy peptone, sodium chloride, glucose, lactose, sodium dihydrogen phosphate, Pig cholate, mannitol, Sodium Pyruvate,
Glycine, 2- hydroxyphenyls-β-D-RIBOSE glycosides (catechol-β-D-ribose glycosides), mango extract solution, molysite, surfactant
It is well mixed in water, after being well mixed, adjusts pH most 7.3；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 2 × 105Sterilized 20 minutes at Pa, 121 DEG C of temperature, then
50 DEG C are naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Embodiment 2
The culture medium of microorganism in a kind of detection blood of human body, including basal medium and additive, in terms of mass fraction,
The additive includes following material：
6 parts of blood peptone, 3 parts of sodium chloride, 1.2 parts of glucose, 0.8 part of lactose, 1.8 parts of disodium hydrogen phosphate, Pig cholate
0.02 part, 1.5 parts of mannitol, 1.5 parts of Sodium Pyruvate, 1.9 parts of Pidolidone, magenta-β-D-ribose glycosides (the chloro- 3- Yin of the bromo- 6- of 5-
Diindyl epoxide-β-D-ribose glycosides) 2 parts, 1.2 parts of mango extract solution, 0.05 part of zinc salt, 0.02 part of surfactant, pH value 7.2,
1000 parts of distilled water；
The present embodiment additionally provides the preparation method of the culture medium of microorganism in the detection blood of human body, including following step
Suddenly：
(1) by blood peptone, sodium chloride, glucose, lactose, disodium hydrogen phosphate, Pig cholate, mannitol, Sodium Pyruvate,
Pidolidone, magenta-β-D-ribose glycosides (the chloro- 3- indoxyls-β of the bromo- 6- of 5--D-ribose glycosides), mango extract solution, zinc salt, table
Face activating agent is well mixed in water, after being well mixed, adjusts pH most 7.2；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 2 × 105Sterilized 25 minutes at Pa, 121 DEG C of temperature, then
50 DEG C are naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Embodiment 3
The culture medium of microorganism in a kind of detection blood of human body, including basal medium and additive, in terms of mass fraction,
The additive includes following material：
12 parts of tryptone, 5.8 parts of sodium chloride, 2.8 parts of glucose, 3.5 parts of lactose, 3.2 parts of dipotassium hydrogen phosphate, Pig cholate
0.06 part, 4.2 parts of mannitol, 2.2 parts of Sodium Pyruvate, 4.3 parts of Glu, dihydroxyflavone-β -6 parts of D-ribose glycosides, awns
2.3 parts of fruit extract solution, 0.36 part of cobalt salt, 0.03 part of surfactant, pH value 7.5,1000 parts of distilled water；
The present embodiment additionally provides the preparation method of the culture medium of microorganism in the detection blood of human body, including following step
Suddenly：
(1) by tryptone, sodium chloride, glucose, lactose, dipotassium hydrogen phosphate, Pig cholate, mannitol, Sodium Pyruvate, L-
Glutamine, dihydroxyflavone-β-D-ribose glycosides, mango extract solution, cobalt salt, surfactant are well mixed in water, mixing
After uniformly, pH most 7.5 is adjusted；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 1 × 105Sterilized 30 minutes at Pa, 122 DEG C of temperature, then
60 DEG C are naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Embodiment 4
The culture medium of microorganism in a kind of detection blood of human body, including basal medium and additive, in terms of mass fraction,
The additive includes following material：
5 parts of meat peptone, 2.5 parts of sodium chloride, 1 part of glucose, 0.5 part of lactose, 1.5 parts of phosphorus source, 0.01 part of Pig cholate are sweet
Reveal 0.5 part of alcohol, 1 part of Sodium Pyruvate, 1 part of TYR, X- β-D-ribose glycosides (the chloro- 3- indoxyls-β-D-riboses of the bromo- 4- of 5-
Glycosides) 1 part, 1 part of mango extract solution, 0.02 part of cobalt salt, 0.01 part of surfactant, pH value 7.4,1000 parts of distilled water；
The present embodiment additionally provides the preparation method of the culture medium of microorganism in the detection blood of human body, including following step
Suddenly：
(1) by meat peptone, sodium chloride, glucose, lactose, phosphorus source, Pig cholate, mannitol, Sodium Pyruvate, L- junket ammonia
Acid, X- β-D-ribose glycosides (the chloro- 3- indoxyls-β of the bromo- 4- of 5--D-ribose glycosides), mango extract solution, cobalt salt, surfactant exist
It is well mixed in water, after being well mixed, adjusts pH most 7.4；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 3 × 105Sterilized 23 minutes at Pa, 121 DEG C of temperature, then
49 DEG C are naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Embodiment 5
The culture medium of microorganism in a kind of detection blood of human body, including basal medium and additive, in terms of mass fraction,
The additive includes following material：
15 parts of soy peptone, 7.5 parts of sodium chloride, 13 parts of glucose, 4 parts of lactose, 3.5 parts of potassium dihydrogen phosphate, Pig cholate
0.08 part, 6 parts of mannitol, 3 parts of Sodium Pyruvate, 6 parts of 1B, pink-β-D-ribose glycosides (the chloro- 3- indoxyl-β-D- of 6-
Riboside) 8 parts, 3 parts of mango extract solution, 0.58 part of chromic salts, 0.05 part of surfactant, pH value 7.5,1000 parts of distilled water；
The present embodiment additionally provides the preparation method of the culture medium of microorganism in the detection blood of human body, including following step
Suddenly：
(1) by soy peptone, sodium chloride, glucose, lactose, potassium dihydrogen phosphate, Pig cholate, mannitol, Sodium Pyruvate,
1B, pink-β-D-ribose glycosides (the chloro- 3- indoxyls-β of 6--D-ribose glycosides), mango extract solution, chromic salts, surface-active
Agent is well mixed in water, after being well mixed, adjusts pH most 7.5；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 3 × 105Sterilized 30 minutes at Pa, 120 DEG C of temperature, then
60 DEG C are naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
Foregoing description is only the description to section Example of the present invention, not to any restriction of the scope of the invention, one's own profession
The those of ordinary skill of industry can make improvement or modification according to the present invention to above-described embodiment, but belong to present invention protection model
Enclose.
Claims (9)
1. a kind of culture medium for detecting microorganism in blood of human body, it is characterised in that including basal medium and additive, with matter
Number meter is measured, the additive includes following material：Peptone 5-15 parts, sodium chloride 2.5-7.5 parts, glucose 1-3 parts, lactose
0.5~4 part, phosphorus source 1.5-3.5 parts, 0.01~0.08 part of Pig cholate, 0.5~6 part of mannitol, Sodium Pyruvate 1-3 parts, amino acid
1~6 part, β -1~8 part of riboside zymolyte, 1~3 part of mango extract solution, 0.02~0.58 part of metal salt, surfactant
0.01~0.05 part, 1000 parts of distilled water.
2. the culture medium of microorganism in detection blood of human body according to claim 1, it is characterised in that with mass fraction
Meter, the additive include following material：6~12 parts of peptone, 3~5.8 parts of sodium chloride, 1.2~2.8 parts of glucose, lactose
0.8~3.5 part, 1.8~3.2 parts of phosphorus source, 0.02~0.06 part of Pig cholate, 1.5~4.2 parts of mannitol, Sodium Pyruvate 1.5~
2.2 parts, amino acid/11 .9~4.3 part, β -2~6 parts of riboside zymolyte, 1.2~2.3 parts of mango extract solution, metal salt 0.05~
0.36 part, 0.02~0.03 part of surfactant, 1000 parts of distilled water.
3. the culture medium of microorganism in detection blood of human body according to claim 2, it is characterised in that with mass fraction
Meter, the additive include following material：10 parts of peptone, 4.8 parts of sodium chloride, 2.3 parts of glucose, 3.2 parts of lactose, phosphorus source
2.4 parts, 0.05 part of Pig cholate, 3.6 parts of mannitol, Sodium Pyruvate 1-3 parts, 2.6 parts of amino acid, β -4 parts of riboside zymolyte, awns
1.8 parts of fruit extract solution, 0.16 part of metal salt, 0.02 part of surfactant, 1000 parts of distilled water.
4. the culture medium of microorganism in the detection blood of human body according to claims 1 to 3 any one, it is characterised in that
The peptone is at least one of soy peptone, blood peptone, tryptone, meat peptone.
5. the culture medium of microorganism in detection blood of human body according to claim 4, it is characterised in that phosphorus source is phosphorus
At least one of acid dihydride sodium, disodium hydrogen phosphate, dipotassium hydrogen phosphate and potassium dihydrogen phosphate.
6. the culture medium of microorganism in detection blood of human body according to claim 5, it is characterised in that the amino acid is
Glycine, ALANINE, Pidolidone, Glu, L-arginine, L-Histidine, L-Leu, ILE, L-
Serine, L-threonine, TYR, 1B, L-Methionine, L-phenylalanine, L-PROLINE, L-Trp, L- figured silk fabrics
At least one of propylhomoserin, L-Aspartic acid and altheine.
7. the culture medium of microorganism in detection blood of human body according to claim 1, it is characterised in that the metal salt is
At least one of molysite, mantoquita, zinc salt, cobalt salt, manganese salt, chromic salts, selenium salt, nickel salt, molybdenum salt, vanadic salts, pink salt, strontium salt.
8. the culture medium of microorganism in the detection blood of human body according to claim 1 or 7, it is characterised in that the β-core
Glucosides zymolyte is 2- hydroxyphenyls-β-D-RIBOSE glycosides (catechol-β-D-ribose glycosides), magenta-β-(5- is bromo- for D-ribose glycosides
The chloro- 3- indoxyls-β of 6--D-ribose glycosides), dihydroxyflavone-β-D-ribose glycosides；X- β-D-ribose glycosides (the chloro- 3- Yin of the bromo- 4- of 5-
Diindyl epoxide-β-D-ribose glycosides), pink-β-D-ribose glycosides (the chloro- 3- indoxyls-β of 6--D-ribose glycosides), the bromo- 3- indoles oxygen of 6-
Base-β-D-ribose glycosides, 5- bromo- 3- indoxyls-β-D-ribose glycosides, the fluoro- 3- indoxyls-β of 6--D-ribose glycosides；Alizarin-β-D-
Riboside, (P)-nitrobenzophenone-β-D-ribose glycosides, 4-methyl umbelliferone base-β-D-ribose glycosides, naphthols-β-D-ribose glycosides, dichloro
At least one of aminophenyl-β-D-ribose glycosides.
A kind of 9. preparation side of the culture medium of microorganism in detection blood of human body according to claim 1~8 any one
Method, it is characterised in that comprise the following steps：
(1) by peptone, sodium chloride, glucose, lactose, phosphorus source, Pig cholate, mannitol, Sodium Pyruvate, amino acid, β-glucose
Glycosides, mango extract solution, metal salt, surfactant are well mixed in water, after being well mixed, adjust pH most 7.2~7.5；
(2) culture medium is put into high-pressure sterilizing pot, in pressure 1~3 × 105Sterilized at Pa, 121 DEG C ± 1 DEG C of temperature 20-30 points
Clock, 45~60 DEG C are then naturally cooled to, obtains nutrient solution；
(3) above-mentioned nutrient solution is well mixed with basal medium, obtains detecting the culture medium of microorganism in blood of human body.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108314193A (en) * | 2018-01-27 | 2018-07-24 | 中国科学院成都生物研究所 | A kind of activation accelerating agent improving cleaning agent waste water psychrophile treatment effect |
| CN112481349A (en) * | 2020-12-18 | 2021-03-12 | 江苏中盛医学诊断试剂有限公司 | Reagent for blood culture bottle production and preparation method |
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| CN102703565A (en) * | 2012-05-21 | 2012-10-03 | 广东环凯微生物科技有限公司 | Chromogenic culture medium for separating and detecting shigella |
| CN104388521A (en) * | 2014-11-24 | 2015-03-04 | 苏州嘉禧萝生物科技有限公司 | Chromogenic medium used for detecting escherichia coli O157:H7 |
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| CN102703565A (en) * | 2012-05-21 | 2012-10-03 | 广东环凯微生物科技有限公司 | Chromogenic culture medium for separating and detecting shigella |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108314193A (en) * | 2018-01-27 | 2018-07-24 | 中国科学院成都生物研究所 | A kind of activation accelerating agent improving cleaning agent waste water psychrophile treatment effect |
| CN108314193B (en) * | 2018-01-27 | 2020-06-09 | 中国科学院成都生物研究所 | Activation promoter for improving low-temperature microbial treatment effect of cleaning agent wastewater |
| CN112481349A (en) * | 2020-12-18 | 2021-03-12 | 江苏中盛医学诊断试剂有限公司 | Reagent for blood culture bottle production and preparation method |
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