CN101089191A - Enriched medium for blood sampler typhoid bacillus - Google Patents
Enriched medium for blood sampler typhoid bacillus Download PDFInfo
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- CN101089191A CN101089191A CN 200610044870 CN200610044870A CN101089191A CN 101089191 A CN101089191 A CN 101089191A CN 200610044870 CN200610044870 CN 200610044870 CN 200610044870 A CN200610044870 A CN 200610044870A CN 101089191 A CN101089191 A CN 101089191A
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- sodium
- cholate
- peptone
- calf serum
- chlor
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Abstract
This invention relates to enrichment medium of blood sample of salmonella typhi and salmonella paratyphi. It contains: (1) sodium citrate and cholate for inhibiting the bulk of gram-negative bacterium, but having no or less inhibiting effect to salmonella typhi, (2), potassium aluminum salfate for activating the activity of salmonella typhi to increase the growth; (3), sodium chloride for regulating osmotic pressure; (4), beef extract, peptone, calf serum and glucose used as nutrition and pracing constituents.
Description
Technical field the present invention relates to a kind of enriched medium for blood sampler typhoid bacillus, and its main component is that extractum carnis, peptone, cholate, potassium aluminium sulfate, sodium-chlor, calf serum, glucose, Sodium Citrate are made through certain technology.Blood supply liquid increases bacterium and cultivates typhoid fever, and paratyphosum Bacterium is used.Utilize culture experiment to differentiate the kind of bacterium, it is accurate, quick, easy that its key is.And the bacterial load size can directly influence detected result.Often need that sample is increased bacterium by different methods and cultivate, carry out the discriminating of bacterial species, this is the most frequently used means of present Microbiological Lab.Because at present the range of application of culture condition is limit, blood supply fluid samples Corynebacterium diphtheriae increases the substratum that bacterium cultivates does not have specific, has that required inoculum size is big, incubation time is grown, poor specificity, is prone to problems such as false positive or false negative.Along with the progress of biological study, the enrichment medium that bacterium is differentiated is studied also in constantly developing both at home and abroad.Biological worker wishes to have always and a kind ofly can overcome above-mentioned insufficient enrichment medium----enriched medium for blood sampler typhoid bacillus.
Background technology the object of the present invention is to provide and a kind ofly can overcome the existing insufficient enriched medium for blood sampler typhoid bacillus of substratum.Similar substratum with other is compared possesses following characteristics: required inoculum size is little, and a small amount of bacterium can breed rapidly, under room temperature usually or 37 ℃ cultivate and got final product observations in 7 days; High specificity is not prone to false positive and false negative.
Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of enriched medium for blood sampler typhoid bacillus.
The prescription following (component and weight percent % thereof) that the present invention selects: extractum carnis (0.4-0.6), calf serum (0.4-0.6), peptone (0.8-1.0), sodium-chlor (0.4-0.6), cholate (0.2-0.4), potassium aluminium sulfate (0.2-0.3), glucose (1.8-2.0), 20% Sodium Citrate 2.0-3.0ml, distilled water add to 100ml.
Basal culture medium contains Sodium Citrate, cholate can suppress most of gram negative bacilluses, and very little to Corynebacterium diphtheriae unrestraint effect or restraint; Potassium aluminium sulfate can activate the Corynebacterium diphtheriae activity, and growth population is increased greatly; Sodium-chlor is used to adjust osmotic pressure; Extractum carnis, peptone, calf serum, glucose are the nutrition in the substratum and support composition.
The preparation method of product of the present invention is:
1), extractum carnis, peptone, cholate, potassium aluminium sulfate, sodium-chlor are mixed in the water, heating for dissolving is corrected pH to 7.8, is sub-packed in the saline bottle wrapping bottleneck, autoclaving 1.05kg30 minute;
2), get supernatant liquor with siphonage, its total amount adds glucose and Sodium Citrate according to the above ratio according to quantity, corrects pH to 7.6;
3), behind the mentioned component mixed dissolution, autoclaving 0.70kg20 minute;
4), under the aseptic condition, add aseptic calf serum, packing ampere bottle, every bottle of about 5ml is stored in the refrigerator standby.
Aseptic technique takes venous patient blood to inject enriched medium for blood sampler typhoid bacillus immediately, increasing bacterium cultivates, in room temperature or 37 ℃ of incubators, to increase the fungus culture transferring every day on blood agar plate and discriminating blood agar plate substratum, and proceed to cultivate and identify, increase the bacterium cultivation and report the result after 7 days.
The present invention has the following advantages: (1) required inoculum size is little, and a small amount of bacterium can breed rapidly; (2) high specificity is not prone to false positive or false negative.
The present invention is described in further detail below in conjunction with embodiment for embodiment:
Be configured according to following table institute column data (weight percent %) and described step thereof:
Prescription one:
Extractum carnis 0.4-0.6
Calf serum 0.4-0.6
Peptone 0.8-1.0
Sodium-chlor 0.4-0.6
Cholate 0.2-0.4
Potassium aluminium sulfate 0.2-0.3
Glucose 1.8-2.0
20% Sodium Citrate 2.0-3.0ml
Distilled water adds to 100ml
Prescription two.
Extractum carnis 0.5-0.6
Calf serum 0.5-0.6
Peptone 0.9-1.0
Sodium-chlor 0.5-0.6
Cholate 0.3-0.4
Potassium aluminium sulfate 0.25-0.3
Glucose 1.9-2.0
20% Sodium Citrate 2.5-3.0ml
Distilled water adds to 100ml
Prescription three:
Extractum carnis 0.4-0.5
Calf serum 0.4-0.5
Peptone 0.8-0.9
Sodium-chlor 0.4-0.5
Cholate 0.2-0.3
Potassium aluminium sulfate 0.2-0.28
Glucose 1.8-1.9
20% Sodium Citrate 2.0-2.5ml
Distilled water adds to 100ml
Prescription four:
Extractum carnis 0.45-0.55
Calf serum 0.45-0.55
Peptone 0.85-0.95
Sodium-chlor 0.45-0.55
Cholate 0.25-0.35
Potassium aluminium sulfate 0.22-0.28
Glucose 1.85-1.9
20% Sodium Citrate 2.2-2.28ml
Distilled water adds to 100ml
Claims (2)
1, a kind of enriched medium for blood sampler typhoid bacillus is to be main component with extractum carnis, peptone, cholate, potassium aluminium sulfate, sodium-chlor, calf serum, glucose, Sodium Citrate; It is characterized in that having following prescription (component and weight percent % thereof): extractum carnis (0.4-0.6), calf serum (0.4-0.6), peptone (0.8-1.0), sodium-chlor (0.4-0.6), cholate (0.2-0.4), potassium aluminium sulfate (0.2-0.3), glucose (1.8-2.0), 20% Sodium Citrate 2.0-3.0ml, distilled water add to 100ml.
2, the preparation method of the described enriched medium for blood sampler typhoid bacillus of a kind of claim 1 is characterized in that having following step:
1), extractum carnis, peptone, cholate, potassium aluminium sulfate, sodium-chlor are mixed in the water, heating for dissolving is corrected pH to 7.8, is sub-packed in the saline bottle wrapping bottleneck, autoclaving 1.05kg30 minute;
2), get supernatant liquor with siphonage, its total amount adds glucose and Sodium Citrate according to the above ratio according to quantity, corrects pH to 7.6;
3), behind the mentioned component mixed dissolution, autoclaving 0.70kg20 minute;
4), under the aseptic condition, add aseptic calf serum, packing ampere bottle, every bottle of about 5ml is stored in the refrigerator standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610044870 CN101089191A (en) | 2006-06-16 | 2006-06-16 | Enriched medium for blood sampler typhoid bacillus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610044870 CN101089191A (en) | 2006-06-16 | 2006-06-16 | Enriched medium for blood sampler typhoid bacillus |
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| Publication Number | Publication Date |
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| CN101089191A true CN101089191A (en) | 2007-12-19 |
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| CN 200610044870 Pending CN101089191A (en) | 2006-06-16 | 2006-06-16 | Enriched medium for blood sampler typhoid bacillus |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824460A (en) * | 2009-03-04 | 2010-09-08 | 王洪林 | Enrichment culture medium for detection, selection of pathogenic bacteria of manure in health examination |
| CN113150995A (en) * | 2021-04-22 | 2021-07-23 | 贵州安康医学检验中心有限公司 | Bacterium transferring and preserving culture medium used in transferring process and preparation method thereof |
| CN113684152A (en) * | 2021-08-30 | 2021-11-23 | 贵州安康医学检验中心有限公司 | Bacteria preservation culture medium and preparation method thereof |
-
2006
- 2006-06-16 CN CN 200610044870 patent/CN101089191A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824460A (en) * | 2009-03-04 | 2010-09-08 | 王洪林 | Enrichment culture medium for detection, selection of pathogenic bacteria of manure in health examination |
| CN113150995A (en) * | 2021-04-22 | 2021-07-23 | 贵州安康医学检验中心有限公司 | Bacterium transferring and preserving culture medium used in transferring process and preparation method thereof |
| CN113684152A (en) * | 2021-08-30 | 2021-11-23 | 贵州安康医学检验中心有限公司 | Bacteria preservation culture medium and preparation method thereof |
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Addressee: Han Fangqi Document name: Notification of Publication of the Application for Invention |
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