CN100495031C - Method for culturing pollen for detecting organic fertilizer decomposing degree - Google Patents
Method for culturing pollen for detecting organic fertilizer decomposing degree Download PDFInfo
- Publication number
- CN100495031C CN100495031C CNB2006100133564A CN200610013356A CN100495031C CN 100495031 C CN100495031 C CN 100495031C CN B2006100133564 A CNB2006100133564 A CN B2006100133564A CN 200610013356 A CN200610013356 A CN 200610013356A CN 100495031 C CN100495031 C CN 100495031C
- Authority
- CN
- China
- Prior art keywords
- pollen
- fertilizer
- culture media
- nutrient culture
- pollen tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Fertilizers (AREA)
Abstract
A method for detecting rotted degree of organic fertilizer by means of pollen - culturing includes culturing eggplant pollen to let it germinate and grow to be pollen tube, comparing elongation length of pollen tube with that of blank pollen tube for judging rotted degree of organic fertilizer and enabling to obtain detection result within 20 - 24 hours.
Description
Technical field
The present invention relates to a kind of biological detecting method of fertilizer rotten degree, the pollen that particularly relates to a kind of fertilizer rotten degree is cultivated detection method.
Background technology
Organic fertilizer is a kind of be mainly derived from natural plants and/or animal, and imposing on soil is the material of its major function so that plant nutrient to be provided.This fertilizer needs to be handled by fermentation and just can be applied to plant after reaching certain rotten degree by composting material, otherwise its inner objectionable impurities can have a negative impact to the growth of plant, thereby the trace routine of rotten degree is an important key link in the compost process.Present fertilizer rotten degree detection method mainly is divided into physics, chemistry, instrument and biological method, wherein physics, chemistry and instrument detecting method generally can only be carried out qualitative or quantitative test to a certain index of organic fertilizer, and the suitable kind of composting material is restricted, and effort, time-consuming, cost is high.Biological detecting method then has advantages such as the comparatively reliable and suitable composting material scope of comprehensive detection result is wide comparatively speaking.It is the seed germination experiment method that at present external organic fertilizer production producer uses more fertilizer rotten degree biological detecting method.Though this test method(s) all is suitable for for the fertilizer rotten degree detection of compostings such as animal waste, municipal refuse, mud and the broken branches and fallen leaves, and it is to utilize plant itself to come that each plant growth in the organic fertilizer production process is encumbered material to carry out comprehensive detection, thereby testing result is comparatively reliable.But there is following point in the germination detection method of this fertilizer rotten degree: the one, be subjected to the influence of full degree of seed and individual size, and there are certain difference in the germination speed of different seeds and the elongation of root; The 2nd, its judgement to the high fertilizer rotten degree of salt content is relatively more difficult, and the high organic fertilizer of indivedual salt content can be influential for the testing result of rotten degree; The 3rd, it is longer to detect the required time, needs 3～7 days usually, promptly 3 days survey germination percentages, 7 days long indexes of survey root.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide the pollen of a kind of applied widely, method of operating simple and fast, the result is accurate and cost is low fertilizer rotten degree to cultivate detection method.
In order to achieve the above object, the pollen of fertilizer rotten degree provided by the invention cultivation detection method is to carry out according to the following step order:
(1) gathers the eggplant just opened and the pollen of camellia respectively, it is screened by 100 mesh sieves, put into plastic bottle with template parcel back with drying agent then, preserve standby down in-15 ℃;
(2) fertilizer sample and the distilled water weight ratio with 1: 2 is mixed, and vibration 30 minutes in 60 ℃ water-bath, separated 30 minutes with hydro-extractor then, get supernatant as the fertilizer leaching liquor;
(3) respectively with agar, sucrose, boron, distilled water and fertilizer leaching liquor mix according to the ratio of 0.8g: 10g: 16mg/l: 82.9g: 6.3g and 0.8g: 10g: 10mg/l: 82.9g: 6.3g, with the HCl of 0.1N or NaOH the pH value of preceding a kind of potpourri is transferred to 6.0 then, and the pH value of a kind of potpourri in back is transferred to 7.0, and being lower than heating makes its fusing under 60 ℃ the temperature, draw the liquid medium after the 20ml fusing and inject the double dish of diameter 12cm, cooling back and make eggplant pollen nutrient culture media and the camellia pollen nutrient culture media that is added with the fertilizer leaching liquor respectively;
(4) make eggplant pollen or camellia pollen be distributed on the template even, thinly, the section of cover glass is moved gently along the template surface of spreading above-mentioned pollen, so that the section of cover glass is stained with pollen on a small quantity, equably, the cover glass section that will speckle with eggplant or camellia pollen is then planted a barriness chalk line being stained with gently on above-mentioned chilled eggplant pollen nutrient culture media and the camellia pollen media surface respectively on corresponding media surface;
(5) the above-mentioned nutrient culture media that is implanted with the pollen line is placed under 25 ℃ the temperature and cultivated 20 hours so that pollen tube from the surface of nutrient culture media along growing to both sides with the rectangular direction of the pollen line of implanting;
(6) with the blank pollen tube length on the nutrient culture media that does not add the fertilizer leaching liquor under the same terms as benchmark, if above-mentioned pollen tube length is less than blank pollen tube length, show that this fertilizer does not become thoroughly decomposed as yet fully, and if above-mentioned pollen tube length more than or equal to blank pollen tube length, shows that then this fertilizer becomes thoroughly decomposed.
The rotating speed of described hydro-extractor is 6000 rev/mins.
It is to make its germination, grow into pollen tube by the cultivation to eggplant or camellia pollen that the pollen of fertilizer rotten degree provided by the invention is cultivated detection method, compare the rotten degree of judging fertilizer with the extended length of pollen tube and the extended length of blank pollen tube then, it is not only applicable to all materials of producing fertilizer, and only needed 20～24 hours just can obtain testing result, thereby the method for operating simple and fast, the batch production that therefore is particularly suitable for discarded object is handled.In addition, the individual difference coefficient of alteration of pollen is little, and is not subjected to the influence of full degree, individual size differences.Particularly the physiological property of pollen tube and root hair are quite similar, compare its germination to crop with the seed germination experiment method, the growth objectionable impurities is more responsive, thereby test result accurately and reliably, thereby can guarantee the safe handling of fertilizer.
Embodiment
The pollen that describes fertilizer rotten degree provided by the invention below in conjunction with specific embodiment in detail is cultivated detection method.
Embodiment 1:
At first 300g is made up of chicken manure, cow dung and sawdust, and pile in the rotten fermentation tank the 1st fermentation 7 days at aeration type, stack then 6 months with carry out the 2nd time the fermentation fertilizer sample and 600g distilled water mix, and in 60 ℃ water-bath, vibrated 30 minutes, separated 30 minutes under 6000 rev/mins rotating speed with hydro-extractor then, therefrom take out the 6.3g supernatant as this fertilizer leaching liquor, add 0.8g agar, 10g sucrose, 16mg/l boron and 82.9g distilled water then and mix.Use the HCl of 0.1N or NaOH that it is adjusted to 6.0 according to the pH value of potpourri then, and being lower than heating makes its fusing under 60 ℃ the temperature, draw the liquid medium after the 20ml fusing and inject the double dish of diameter 12cm, cooling back and make the eggplant pollen nutrient culture media that is added with the fertilizer leaching liquor.Be distributed in the eggplant pollen of gathering that just opened, that screen and under-15 ℃, preserve by 100 mesh sieves on the template even, thinly, the section of cover glass is moved gently along the template surface of spreading above-mentioned eggplant pollen, so that the section of cover glass is stained with pollen on a small quantity, equably, the cover glass section that will speckle with eggplant pollen then is stained with gently on above-mentioned chilled eggplant pollen media surface and is being planted a barriness chalk line on this surface; The above-mentioned nutrient culture media that is implanted with the pollen line placed under 25 ℃ the temperature and cultivated 20 hours, because pollen tube growth has taxis, so pollen tube can be from the surface of nutrient culture media along growing to both sides with the rectangular direction of the pollen line of implanting, the length of cultivating the back pollen tube is 3.76mm; Do not add under the same conditions simultaneously on the nutrient culture media of fertilizer leaching liquor and repeat above-mentioned process of the test, and with the pollen tube that grows as blank pollen tube, the length that this moment should the blank pollen tube is 3.08mm.Because the pollen tube length on the nutrient culture media of interpolation fertilizer leaching liquor shows that greater than blank pollen tube length this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 2:
Fertilizer sample among the embodiment 1 become by the bark of chicken manure and pulverizing form, the raw material that processing mode and fermentation time are identical, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is 4.20mm, and the length of blank pollen tube is 3.08mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 3:
Fertilizer sample among the embodiment 1 is become through 6 months rotten paper mill sludges of heap, and nutrient culture media and the incubation used are identical, and the length of cultivating the back pollen tube is 3.32mm, and the length of blank pollen tube is 2.89mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 4:
Fertilizer sample among the embodiment 1 is become through 3 months rotten straw that ferment of heap, and nutrient culture media and the incubation used are identical, and the length of cultivating the back pollen tube is 3.43mm, and the length of blank pollen tube is 3.12mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 5:
Fertilizer sample among the embodiment 1 is become the fresh pig dung of taking from the feace storing pool on pig farm, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is 2.43mm, and the length of blank pollen tube is 3.08mm.Owing to add pollen tube length on the nutrient culture media of this fertilizer leaching liquor less than blank pollen tube length, show that this fertilizer does not become thoroughly decomposed as yet fully, if use can to crop emerge and the generation of grow encumbers.
Embodiment 6:
Fertilizer sample among the embodiment 1 is become respectively through 6 days rotten pig manure, chicken manure and cow dungs of fermenting of heap, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is respectively 1.17,1.45 and 2.40mm, and the length of blank pollen tube then is respectively 2.67,2.42 and 2.73mm.Because the pollen tube length on the nutrient culture media of above-mentioned interpolation fertilizer leaching liquor shows that respectively less than corresponding blank pollen tube length these fertilizers do not become thoroughly decomposed as yet fully, if use can to crop emerge and the generation of grow encumbers.
Embodiment 7:
At first 300g is made up of chicken manure, cow dung and sawdust, and pile in the rotten fermentation tank the 1st fermentation 7 days at aeration type, stack then 6 months with carry out the 2nd time the fermentation fertilizer sample and 600g distilled water mix, and in 60 ℃ water-bath, vibrated 30 minutes, separated 30 minutes under 6000 rev/mins rotating speed with hydro-extractor then, therefrom take out the 6.3g supernatant as this fertilizer leaching liquor, add 0.8g agar, 10g sucrose, 10mg/l boron and 82.9g distilled water then and mix.Use the HCl of 0.1N or NaOH that it is adjusted to 7.0 according to the pH value of potpourri then, and being lower than heating makes its fusing under 60 ℃ the temperature, draw the liquid medium after the 20ml fusing and inject the double dish of diameter 12cm, cooling back and make the camellia pollen nutrient culture media that is added with the fertilizer leaching liquor.Be distributed in the camellia pollen of gathering that just opened, that screen and under-15 ℃, preserve by 100 mesh sieves on the template even, thinly, the section of cover glass is moved gently along the template surface of spreading above-mentioned camellia pollen, so that the section of cover glass is stained with pollen on a small quantity, equably, the cover glass section that will speckle with camellia pollen then is stained with gently on above-mentioned chilled camellia pollen media surface and is being planted a barriness chalk line on this surface; The above-mentioned nutrient culture media that is implanted with the pollen line placed under 25 ℃ the temperature and cultivated 20 hours, because pollen tube growth has taxis, so pollen tube can be from the surface of nutrient culture media along growing to both sides with the rectangular direction of the pollen line of implanting, the length of cultivating the back pollen tube is 2.60mm; Do not add under the same conditions simultaneously on the nutrient culture media of fertilizer leaching liquor and repeat above-mentioned process of the test, and with the pollen tube that grows as blank pollen tube, the length that this moment should the blank pollen tube is 2.56mm.Because the pollen tube length on the nutrient culture media of this fertilizer leaching liquor of interpolation shows that greater than blank pollen tube length this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 8:
Fertilizer sample among the embodiment 7 become by the bark of chicken manure and pulverizing form, the raw material that processing mode and fermentation time are identical, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is 2.62mm, and the length of blank pollen tube is 2.56mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 9:
Fertilizer sample among the embodiment 7 is become through 6 months rotten paper mill sludges of heap, and nutrient culture media and the incubation used are identical, and the length of cultivating the back pollen tube is 2.82mm, and the length of blank pollen tube is 2.63mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 10:
Fertilizer sample among the embodiment 7 is become through 3 months rotten straw that ferment of heap, and nutrient culture media and the incubation used are identical, and the length of cultivating the back pollen tube is 2.91mm, and the length of blank pollen tube is 2.68mm, show that this fertilizer becomes thoroughly decomposed, thereby can safely use.
Embodiment 11:
Fertilizer sample among the embodiment 7 is become the fresh pig dung of taking from the feace storing pool on pig farm, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is 1.42mm, and the length of blank pollen tube is 2.44mm.Owing to add pollen tube length on the nutrient culture media of this fertilizer leaching liquor less than blank pollen tube length, show that this fertilizer does not become thoroughly decomposed as yet fully, if use can to crop emerge and the generation of grow encumbers.
Embodiment 12:
Fertilizer sample among the embodiment 7 is become respectively through 6 days rotten pig manure, chicken manure and cow dungs of fermenting of heap, and nutrient culture media and the incubation used are identical, the length of cultivating the back pollen tube is respectively 2.23,2.00 and 2.06mm, and the length of blank pollen tube then is respectively 2.70,2.52 and 2.55mm.Because the pollen tube length on the nutrient culture media of above-mentioned interpolation fertilizer leaching liquor shows that respectively less than corresponding blank pollen tube length these fertilizers do not become thoroughly decomposed as yet fully, if use can to crop emerge and the generation of grow encumbers.
Claims (2)
1, a kind of pollen of fertilizer rotten degree is cultivated detection method, it is characterized in that: it is to carry out according to the following step order that the pollen of described fertilizer rotten degree is cultivated detection method:
(1) gathers the eggplant just opened and the pollen of camellia respectively, it is screened by 100 mesh sieves, put into plastic bottle with template parcel back with drying agent then, preserve standby down in-15 ℃;
(2) fertilizer sample and the distilled water weight ratio with 1: 2 is mixed, and vibration 30 minutes in 60 ℃ water-bath, separated 30 minutes with hydro-extractor then, get supernatant as the fertilizer leaching liquor;
(3) respectively with agar, sucrose, boron, distilled water and fertilizer leaching liquor mix according to the ratio of 0.8g: 10g: 16mg/l: 82.9g: 6.3g and 0.8g: 10g: 10mg/l: 82.9g: 6.3g, with the HCl of 0.1N or NaOH the pH value of preceding a kind of potpourri is transferred to 6.0 then, and the pH value of a kind of potpourri in back is transferred to 7.0, and being lower than heating makes its fusing under 60 ℃ the temperature, draw the liquid medium after the 20ml fusing and inject the double dish of diameter 12cm, cooling back and make eggplant pollen nutrient culture media and the camellia pollen nutrient culture media that is added with the fertilizer leaching liquor respectively;
(4) make eggplant pollen or camellia pollen be distributed on the template even, thinly, the section of cover glass is moved gently along the template surface of spreading above-mentioned pollen, so that the section of cover glass is stained with pollen on a small quantity, equably, the cover glass section that will speckle with eggplant or camellia pollen is then planted a barriness chalk line being stained with gently on above-mentioned chilled eggplant pollen nutrient culture media and the camellia pollen media surface respectively on corresponding media surface;
(5) the above-mentioned nutrient culture media that is implanted with the pollen line is placed under 25 ℃ the temperature and cultivated 20 hours so that pollen tube from the surface of nutrient culture media along growing to both sides with the rectangular direction of the pollen line of implanting;
(6) with the blank pollen tube length on the nutrient culture media that does not add the fertilizer leaching liquor under the same terms as benchmark, if above-mentioned pollen tube length is less than blank pollen tube length, show that this fertilizer does not become thoroughly decomposed as yet fully, and if above-mentioned pollen tube length more than or equal to blank pollen tube length, shows that then this fertilizer becomes thoroughly decomposed.
2, the pollen of fertilizer rotten degree according to claim 1 is cultivated detection method, and it is characterized in that: the rotating speed of described hydro-extractor is 6000 rev/mins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100133564A CN100495031C (en) | 2006-03-23 | 2006-03-23 | Method for culturing pollen for detecting organic fertilizer decomposing degree |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100133564A CN100495031C (en) | 2006-03-23 | 2006-03-23 | Method for culturing pollen for detecting organic fertilizer decomposing degree |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1831534A CN1831534A (en) | 2006-09-13 |
| CN100495031C true CN100495031C (en) | 2009-06-03 |
Family
ID=36993948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100133564A Expired - Fee Related CN100495031C (en) | 2006-03-23 | 2006-03-23 | Method for culturing pollen for detecting organic fertilizer decomposing degree |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100495031C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165865A (en) * | 2010-12-30 | 2011-08-31 | 华南农业大学 | Method for determining decomposition degree of organic fertilizers in municipal sludge by utilizing mainland vegetable seeds |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617619B (en) * | 2009-07-31 | 2011-11-30 | 中国农业大学 | Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof |
| CN101806738B (en) * | 2010-04-27 | 2011-05-25 | 南京农业大学 | Method for fast characterizing compost maturity |
-
2006
- 2006-03-23 CN CNB2006100133564A patent/CN100495031C/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 堆肥腐熟度的研究进展. 李承强，魏源送，樊耀波，王敏健.环境科学进展，第7卷第6期. 1999 |
| 堆肥腐熟度的研究进展. 李承强，魏源送，樊耀波，王敏健.环境科学进展，第7卷第6期. 1999 * |
| 畜禽废弃物堆肥化过程中的腐熟度评价方法. 任顺荣，邵玉翠.天津农业科学，第11卷第3期. 2005 |
| 畜禽废弃物堆肥化过程中的腐熟度评价方法. 任顺荣，邵玉翠.天津农业科学，第11卷第3期. 2005 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102165865A (en) * | 2010-12-30 | 2011-08-31 | 华南农业大学 | Method for determining decomposition degree of organic fertilizers in municipal sludge by utilizing mainland vegetable seeds |
| CN102165865B (en) * | 2010-12-30 | 2012-10-03 | 华南农业大学 | Method for determining decomposition degree of organic fertilizers in municipal sludge by utilizing mainland vegetable seeds |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1831534A (en) | 2006-09-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Meng et al. | Co-composting of the biogas residues and spent mushroom substrate: Physicochemical properties and maturity assessment | |
| CN107244961A (en) | The method that rice seedling culture medium is produced as raw material using Jasmine slag, tea dust | |
| CN110622824A (en) | Vegetable seedling culture medium prepared from agricultural and forestry wastes | |
| CN103396182B (en) | Method for producing solanaceous vegetable seedling growing substrate by utilizing salvaged material of cassava processing | |
| CN105218183A (en) | The production technique of rice seedling raising ground substance | |
| CN104744182A (en) | Tobacco water retention biological organic fertilizer and preparation method thereof | |
| CN108552013A (en) | A kind of preparation method of cultivation matrix and the fermentation determination method of cultivation matrix | |
| CN104261958B (en) | Leaf vegetable cultivation matrix and preparation method thereof | |
| CN105906387A (en) | Paddy rice seedling raising substrate from mushroom residue of flammulina velutipes and preparation method thereof | |
| CN104756843A (en) | Organic seedling substrate for tomato and preparation method thereof | |
| CN100495031C (en) | Method for culturing pollen for detecting organic fertilizer decomposing degree | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN102875207B (en) | Method for preparing light matrix by using ageratina adenophora | |
| CN106631434A (en) | Tomato artificial seedling culture substrate and preparation method thereof | |
| CN110903120A (en) | Production method of organic-inorganic compound fertilizer taking plant straws as raw materials | |
| Cheruiyot et al. | Environmental and economic impacts of biodegradable plastic film mulching on rainfed maize: Evaluations on sustainability and productivity | |
| CN109809947A (en) | A kind of beach saline land rice organic fertilizer | |
| CN115160085A (en) | Organic fertilizer for improving ginseng fruit continuous cropping soil | |
| CN111886960B (en) | Rice straw returning method | |
| CN106416651A (en) | Method for increasing seed germination rate in medium seedling culture | |
| CN111533609A (en) | Saline-alkali soil modifier carbon-based fertilizer and preparation method and application thereof | |
| CN104756842A (en) | Tomato seedling cultivation substrate and production method thereof | |
| Lupwayi et al. | Soil microbial response to wood ash or lime applied to annual crops and perennial grass in an acid soil of northwestern Alberta | |
| CN109627080A (en) | A kind of pocket type fast fermentation method | |
| CN104230417A (en) | Method for rapidly preparing organic fertilizer additive used in low-temperature areas by using cow dung and straw and applications of organic fertilizer additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090603 Termination date: 20130323 |