CN100482690C - Method of coagulating natural rubber fresh latex with fungus culture medium - Google Patents
Method of coagulating natural rubber fresh latex with fungus culture medium Download PDFInfo
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- CN100482690C CN100482690C CNB2005100863030A CN200510086303A CN100482690C CN 100482690 C CN100482690 C CN 100482690C CN B2005100863030 A CNB2005100863030 A CN B2005100863030A CN 200510086303 A CN200510086303 A CN 200510086303A CN 100482690 C CN100482690 C CN 100482690C
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Abstract
The invention discloses a method to screen selecting fungi Trichoriella ornithopoda Oorschot et de Hoog culture water and calcia chloridum mixed freezing natural rubber latex from waste disposal basin of producing natural rubber; which is characterized by the following parts: shortening time of biological freezing; the time of biological freezing shorten from 6 hours to 3min- 2h and rationalize property of product is better than acid freezing. The invention is fit for rubber-clear freezing and freezing of low ammonia latex with ammonia quantity less than 0.04%.
Description
Invention field
The present invention relates to the processing of the dried glue of natural rubber, particularly the natural rubber fresh latex method of solidifying.
Background technology
By the latex of microbial contamination, because of of the decomposition utilization of microorganism growth reproductive process, destroyed the stability of latex to non-rubber component, cause latex go bad, solidify, smelly, this form of solidifying of latex is called natural coagulation.Latex coagulation in the existing production all adopts acid coagulation, promptly adds tart chemical agent (formic acid or acetate) in fresh latex, reduces the pH value of latex, destroy the stability of latex, rubber particles in the latex is assembled mutually, be frozen into piece, it is solid that this form of solidifying is called acid cure.Solidifying of this dual mode, its relative merits is separately arranged, the natural coagulation cost is low, the product of producing has good physicochemical performance and vulcanization characteristics, but setting time is long, and because of microbiological degradation utilizes the glue protein of milk, discharges a kind of unpleasant stench of death flavor, seriously pollute atmosphere, caused abominable production environment.The time weak point that acid cure is solid solidifies fully, but the cost height, the product physicochemical property deviation of production, vulcanization rate is slower.In order to save the solid cost of acid cure, it is slow to overcome natural coagulation speed, the shortcoming that the time is long, smell is smelly, and the research worker of various countries' natural rubber has done a large amount of biological coagulation research work, in the hope of obtaining high-quality rubber product.As far back as the sixties, Malay C.K.JOHN just utilizes molasses and pineapple juice to add to add in the whey that contains microorganism and gives cultivation, be used to solidify natural rubber latex [1.C.K.John, .Biological Coagulation of Hevea Latex Using WasteCarbohydrate Substrates.Joumal of the Rubber Research Institute ofMalaya.1966, Vol19 (5): 286-289], this research work has just utilized the microorganism that exists naturally in the whey, does not have the bacterial classification that autotelic screening helps solidifying fresh latex; The beginning of the eighties at the end of the seventies, the bacterial strain of acid is produced in people such as the Wang Zhongmin of Huanan Tropical Crops Prods. processing Design Inst. screening, separation, utilize isolating bacterial classification at plantation, receive the biological coagulation research of having carried out more detailed system in the glue station (Wang Zhongmin, analogy Meng Jun. the latex microorganism disperses to solidify research. tropical processing of farm products 1998, No2:1-5) this research work focuses on coagulum, and neglects in culture of strains.But, no matter be people's such as C.K.JOHN or Wang Zhongmin research, setting time is at least more than 6 hours, and effect is unsatisfactory.
Summary of the invention
(title: Trichoriella ornithopoda Oorschot et de Hoog) the test tube slant culture was kept at China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) to fungi of the present invention on August 10th, 2005, this centre address is No. 13 Institute of Microorganism, Academia Sinica in north, ZhongGuancun, Haidian District, BeiJing, and preserving number is CGMCC 1435.
The object of the present invention is to provide a kind of method of biological coagulation natural rubber fresh latex.This method is easy and simple to handle, save the energy, reduce and solidify cost, shorten the biological coagulation time, uses this clotting method, and the biological coagulation time shortens to 3 minutes from minimum 6 hours of forefathers' research～2 hours, and its product physicochemical property is than solid good of acid cure.
Specifically, the invention provides a kind of from the mud in natural rubber glue-preparing exhausted water pond, screen identify the cultural method of fungus culture medium of Trichoriella ornithopodaOorschot et de Hoog by name and the method for using this nutrient solution coagulating natural rubber fresh latex through Institute of Microorganism, Academia Sinica, this method is that the test tube slant culture with fungi Trichoriella ornithopodaOorschot et de Hoog inserts in the liquid nutrient medium, after one or more levels liquid culture, again nutrient solution and calcium chloride are mixed into solidification liquid, pour latex into, stir, leave standstill certain hour, latex coagulation becomes piece.The grumeleuse slaking is more than 4 hours, whey in desirable this grumeleuse adds glucide and cultivates, its nutrient solution again with calcium chloride mixing after coagulation fresh latex, so: get that whey → adding carbohydrate material cultivation → nutrient solution mixes → solidify fresh latex with calcium chloride, carry out repeatedly again and again.
Specific embodiments of the present invention is as follows:
The test tube slant culture of fungi Trichoriella ornithopoda Oorschot et de Hoog is connected in the liquid nutrient medium of the present invention, enlarge step by step according to the use scale, the level liquid substratum needs scalding, thereafter what need not boil, insert the upper level nutrient solution by 5%～10% inoculum size, cultivated 1～10 day according to temperature the inoculation back, standby.Before the coagulum, nutrient solution and calcium chloride are mixed into solidification liquid, pour solidification liquid into latex, stir, leave standstill and solidify.The consumption of nutrient solution is 0.5%～5% calculating of dried glue weight in the latex by the sugar degree in the nutrient solution, and the calcium chloride consumption is pressed 0.01%～0.2% calculating of thousand glue weight in the coagulum.
Grumeleuse after solidifying with bio-culture solution, slaking is more than 4 hours, get its whey, press the aforesaid liquid media components and add glucide, cultivated 1～10 day according to temperature, drop into once more and solidify use, the usage quantity of nutrient solution usage quantity and calcium chloride is same as described above, so: get that whey → adding carbohydrate material cultivation → nutrient solution mixes → solidify fresh latex with calcium chloride, carry out repeatedly again and again, sustainable two weeks is until the longer time, when treating that coagulation result does not reach original effect, and the renewed vaccination liquid culture.
The liquid nutrient medium of nutrient solution of the present invention contains following component (in weight part, down together):
Whey, water: 100 parts
Glucide: 1～15 part
The liquid nutrient medium component of nutrient solution of the present invention, as whey or water, preferably one or more of whey and water are consolidated in biological coagulation whey, acid cure, biological coagulation whey most preferably, glucide as biological self reproducing growth nutriment, preferably one or more in molasses, brown sugar, the white sugar, most preferably molasses.
Therefore, most preferably, the liquid nutrient medium of nutrient solution of the present invention contains following component:
Biological coagulation whey: 100 parts
Molasses: 3.5～6 parts
The inventive method can be solidified fresh latex fully at 3 minutes～2 hours, possesses following unusual effect.
---setting time from the biological coagulation of forefathers research shortened to 3 minutes in minimum 6 hours～2 hours, reduced setting time.
---actually operating is easy, save the energy, solidify expense be acid cure solid 1/2nd, reduced production cost, the product physicochemical property has improved the rubber product quality than solid good of acid cure.
---this clotting method is adapted to standard rubbers and disperses to solidify concentrated producing process, provides technical guarantee for caoutchouc standard glue maximizes to produce.
The inventive method also is applicable to rubber-clear freezing.
The inventive method is applicable to that also ammonia quantity is lower than solidifying of 0.04% low-ammonia latex.
(title: Trichoriella ornithopoda Oorschot et de Hoog) the test tube slant culture was kept at China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) to fungi of the present invention on August 10th, 2005, this centre address is No. 13 Institute of Microorganism, Academia Sinica in north, ZhongGuancun, Haidian District, BeiJing, and preserving number is CGMCC 1435.
Embodiment
Embodiment 1
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of biological coagulation wheys, 3.5 parts of molasses, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution is inserted 900ml have been added in the fresh biological coagulation whey of 31.5g molasses, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 20% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 2
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml and have boiled in the liquid nutrient medium that cooling back component is the solid whey of 100 parts of acid cures, 5 parts of molasses, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution access 900ml has been added in the solid whey of fresh acid cure of 45g molasses, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1.25g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 25% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 3
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of water, 6 parts of molasses, under 20 ℃～25 ℃ temperature, cultivated 5 days, this nutrient solution is inserted 900ml have been added in the water of 54g molasses, under 20 ℃～25 ℃ temperature, cultivate 5 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 3g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 30% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 4
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of biological coagulation wheys, 3.5 portions of brown sugar, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution is inserted 900ml have been added in the fresh biological coagulation whey of 31.5g brown sugar, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1200ml cultivates as stated above, add 1g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 20% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 5
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml and have boiled in the liquid nutrient medium that cooling back component is the solid whey of 100 parts of acid cures, 5 portions of brown sugar, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution access 900ml has been added in the solid whey of fresh acid cure of 45g brown sugar, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1.25g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 25% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 6
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of water, 6 portions of brown sugar, under 20 ℃～25 ℃ temperature, cultivated 5 days, this nutrient solution is inserted 900ml have been added in the water of 54g brown sugar, under 20 ℃～25 ℃ temperature, cultivate 5 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 3g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 30% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 7
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of biological coagulation wheys, 6 parts of white sugar, under 20 ℃～25 ℃ temperature, cultivated 5 days, this nutrient solution is inserted 900ml have been added in the fresh biological coagulation whey of 54g white sugar, under 20 ℃～25 ℃ temperature, cultivate 5 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 3g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 30% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 8
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml and have boiled mixture (brown sugar, the molasses ratio: in liquid nutrient medium 2:3) that cooling back component is 100 parts of biological coagulation wheys, 5 portions of brown sugar, molasses, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution is inserted in the fresh biological coagulation whey of mixture that 900ml added 45g brown sugar, molasses, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1.25g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 25% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 9
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml and have boiled in the liquid nutrient medium that cooling back component is 50 parts of biological coagulation wheys, 30 parts of solid wheys of acid cures, 20 parts of water, 5 portions of brown sugar, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution is inserted in the mixing liquid of solid whey of biological coagulation whey, acid cure that 900ml added 45g brown sugar and water (the ratio 5:3:2 of biological coagulation whey, the solid whey of acid cure and water), under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1.25g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 25% fresh latex, stirs, and leaves standstill and solidifies.
Embodiment 10
A, the test tube slant culture of getting a fungi Trichoriella ornithopoda Oorschot et de Hoog insert 100ml, and to have boiled cooling back component be in the liquid nutrient medium of 100 parts of biological coagulation wheys, 2 parts of molasses, 2 portions of brown sugar, 1 part of white sugar, under 28 ℃～31 ℃ temperature, cultivated 2 days, this nutrient solution is inserted in the fresh biological coagulation whey that 900ml added 18g molasses, 18g brown sugar, 9g white sugar, under 28 ℃～31 ℃ temperature, cultivate 2 days again, standby.
B, get the nutrient solution that 1000ml cultivates as stated above, add 1.25g calcium chloride (be made into 10%～20% the aqueous solution) mixing, pouring the 10kg drc into is in 25% fresh latex, stirs, and leaves standstill and solidifies.
Claims (3)
1, a kind of method of coagulating natural rubber fresh latex with fungus culture medium, it is characterized in that the fungi Trichoriellaornithopoda Oorschot et de Hoog test tube slant culture that will screen is connected in the liquid nutrient medium from the mud in natural rubber glue-preparing exhausted water pond, after one or more levels liquid culture, with nutrient solution with become solidification liquid after calcium chloride mixes, pour latex into, stir, leave standstill certain hour, latex coagulation becomes piece; Get the whey in this coagulated mass, add in molasses, brown sugar, the white sugar one or more and cultivate, the nutrient solution that obtains mixes with calcium chloride and solidifies fresh latex; So: get in molasses, brown sugar, the white sugar one or more of whey → add to cultivate → nutrient solution mixes → solidifies fresh latex, carry out repeatedly again and again with calcium chloride.
2, the method for coagulating natural rubber fresh latex with fungus culture medium according to claim 1 is characterized in that described liquid nutrient medium contains following parts by weight of component:
One or more of biological coagulation whey, the solid whey of acid cure and water: 100 parts,
In molasses, brown sugar, the white sugar one or more: 1～15 part.
3, the method for coagulating natural rubber fresh latex with fungus culture medium according to claim 1, the incubation time that it is characterized in that described one or more levels liquid culture are 1～10 day.
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| CN100482690C true CN100482690C (en) | 2009-04-29 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102093492B (en) * | 2009-12-10 | 2014-08-27 | 蒋盛军 | Method for solidifying natural rubber latex by using microbe culture solution |
| CN102485664A (en) * | 2009-12-10 | 2012-06-06 | 蒋盛军 | Method for treating rubber latex solidified waste liquid through microbial fermentation |
| CN102504051B (en) * | 2011-11-10 | 2013-07-03 | 中国热带农业科学院农产品加工研究所 | Method for solidifying nature rubber fresh latex through double parallel-flow and solidification liquid |
| CN102558395B (en) * | 2012-01-06 | 2013-11-06 | 中国热带农业科学院农产品加工研究所 | Biological deodorization method of natural rubber fresh latex biogel block |
| CN102584462B (en) * | 2012-02-26 | 2016-04-20 | 海南医学院 | The glue clean plasm liquid be separated with natural latex coargulation prepares the method for plant nutrition liquid for raw material |
| CN105936882A (en) * | 2016-06-06 | 2016-09-14 | 云南珩森生物科技有限公司 | Microbiota for rapid coagulation of natural latex and use method thereof |
| CN114230690B (en) * | 2021-12-31 | 2023-04-14 | 中国热带农业科学院橡胶研究所 | Preparation method of low-heat-generation high-performance natural rubber |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1222528A (en) * | 1998-11-18 | 1999-07-14 | 中国科学院昆明植物研究所 | Biological solidification technology of natural latex |
| CN1241576A (en) * | 1999-07-23 | 2000-01-19 | 卢创波 | Improved natural latex coagulant |
| JP2001310902A (en) * | 2000-04-28 | 2001-11-06 | Sumitomo Rubber Ind Ltd | Deproteinization agent, deproteinized natural rubber latex using the same and its production |
| CN1392161A (en) * | 2001-06-14 | 2003-01-22 | 上海东化机电工程有限公司 | Natural rubber producing method with lactic acid circulation |
| CN1554673A (en) * | 2003-12-23 | 2004-12-15 | 海南省农恳中心测试站 | Crude rubber latex auxiliary biological solidifying producing method |
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- 2005-08-26 CN CNB2005100863030A patent/CN100482690C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1222528A (en) * | 1998-11-18 | 1999-07-14 | 中国科学院昆明植物研究所 | Biological solidification technology of natural latex |
| CN1241576A (en) * | 1999-07-23 | 2000-01-19 | 卢创波 | Improved natural latex coagulant |
| JP2001310902A (en) * | 2000-04-28 | 2001-11-06 | Sumitomo Rubber Ind Ltd | Deproteinization agent, deproteinized natural rubber latex using the same and its production |
| CN1392161A (en) * | 2001-06-14 | 2003-01-22 | 上海东化机电工程有限公司 | Natural rubber producing method with lactic acid circulation |
| CN1554673A (en) * | 2003-12-23 | 2004-12-15 | 海南省农恳中心测试站 | Crude rubber latex auxiliary biological solidifying producing method |
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