CN100441165C - Cosmetics containing biological active collagen - Google Patents
Cosmetics containing biological active collagen Download PDFInfo
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- CN100441165C CN100441165C CNB2006100211008A CN200610021100A CN100441165C CN 100441165 C CN100441165 C CN 100441165C CN B2006100211008 A CNB2006100211008 A CN B2006100211008A CN 200610021100 A CN200610021100 A CN 200610021100A CN 100441165 C CN100441165 C CN 100441165C
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Abstract
A cosmetic containing bioactive collagen with high effect to beatify and protect skin contains proportionally bioactive collagen, vegetative extract with beautifying and whitening function, and water-soluble moistening and nourishing agent.
Description
Technical field
The present invention relates to a kind of cosmetics that contain biological active collagen.
Background technology
Collagen (collagen protein) is a kind of white, opaque, unbranched fibrous type hmw protein.It is an important protein matter in the animal connective tissue, and the major physiological function is to do the adhesion substance of connective tissue, has the function that supports organ and protection human body, can make skin keep solid and elasticity.Collagen has accounted for 30% of total amount in the interior all proteins of vertebrates body, is main structural protein; Then to have more than 50% be collagen protein to albumen in the skin.
With age, the intravital collagen content of people can run off gradually, the also thickening hardening gradually of mesh-supported body, follow the string, elasticity and the reduction of water conservation degree when skin corium, skin just can follow the string and attenuation and aging occur, reduces but also lipoatrophy, sweat gland and smegma can occur.This can make skin a series of catabiosis such as mottle, wrinkle occur, shows stereotype.
The filamentary structure of collagen protein uniqueness and excellent biological compatibility thereof and low antigenicity make it become the popular research topic of the cosmetics of super quality, functional health product and field of biomedical materials.Collagen protein can be repaired epiderm skin and dermis effectively, promotes the regeneration of collagen in the skin; It is applied to mainly have trophism in the cosmetics, reparation property, moisture retention, compatibility, and characteristics such as affinity.
The epidermis of intact skin is the very fine and close organizational structure of one deck, can only allow water, electrolyte and minority small-molecule substance simple in structure pass through.And the molecular weight of natural collagen is very big, is about 300KD (300,000 dalton), and molecular structure is very complicated, by 3 peptide chain combinations and interwind into right hand triple helix structure.Therefore it has been generally acknowledged that directly adding macromolecular natural collagen in cosmetics is difficult to by skin absorbs.As Wang Xuechuan etc. in " progress of collagen protein and the application in cosmetics thereof " (daily chemical industry, 2005,35 (6): 388-392) in the literary composition, and Li Yunzai " application of collagen protein in foods and cosmetics " (TanJin Agricultural College journal, 2005,12 (2): require molecular weight usually below 2KD when 54-57) all mentioning in the literary composition, think that micromolecular hydrolytic collagen just can penetrate in the skin as the skin type raw material.Therefore, when in the prior art cosmetics, being used as the skin type raw material, all adopt the less hydrolytic collagen of molecular weight, it is joined in the cosmetics such as established cream, frost, emulsion and stir, its consumption in entire product is also little, and general addition is 1-5%.
Up to the present, also not having on the market to occur with the biological active collagen is the cosmetics of primary raw material; More be not that biological activity (natural) collagen about 300KD is the skin-care cosmetics of raw material with the molecular weight.
Summary of the invention
The purpose of this invention is to provide a kind of new cosmetics that contain biological active collagen, these cosmetics are primary raw material with the biological active collagen, have good beauty and skin care effect, and have no adverse reaction.
The technical solution adopted for the present invention to solve the technical problems is: a kind of cosmetics that contain biological active collagen, and it is made up of following components in weight percentage: the plant extract 0-4% of biological active collagen 76-100%, Pear Power effect, water solublity moisturizing agent 0-20%; Wherein, the molecular weight of biological active collagen is 280-300KD, and collagen concentration is 0.05-0.55%.
Wherein, the computing formula of collagen concentration is:
Col in the formula represents collagen concentration in the sample, and A is the content (μ g/ml can adopt spectrophotography to record) of hydroxyproline in the sample, and 7.54 are the computational constant when hydroxyproline is scaled collagen.
The preferred weight percent of above-mentioned each component is: collagen concentration is the biological active collagen 88-99.49% of 0.05-0.55%, plant extract 0.01-2%, the water solublity moisturizing agent 0.5-10% of Pear Power effect.
Wherein, the collagen concentration of biological active collagen is preferably 0.3-0.5%.
The plant extract of Pear Power effect such as Laticauda semifasciata seed extract, Aloe extract, Radix Glycyrrhizae extract, mulberry extract, Semen Vitis viniferae extract etc., preferred Laticauda semifasciata seed extract.
Water solublity moisturizing agent such as glycerol, butylene glycol, polyethylene glycol, glycerol olein etc., preferably glycerine.
In addition, according to the hobby of different consumers, can also contain the used for cosmetic essence of 0.01-0.1% in the above-mentioned cosmetics that contain biological active collagen.
Raw material bioactivity collagen used among the present invention can make from animal skins such as Corii Sus domestica or Corii Bovis seu Bubali by the method that may further comprise the steps:
(1) extraction of solubility in acid natural collagen: get fresh porcine skin/Corii Bovis seu Bubali or salted Corii Sus domestica/Corii Bovis seu Bubali 100 weight portions, wash fleshing with water after, add degreasing agent 2-3 part and handled 3-5 hour, coprocessing 2 times, pull out a mao back split, obtain skin corium, soaked 1 day with 10% saline solution, washing cooling back chopping, get onal piece 10 weight portions of chopping, to 2-4, add proteinase-10 .1-1 part with sour adjust pH, in temperature 4-6 ℃ stir process 1-3 days, obtain not degeneration natural collagen solution of solubility in acid.
(2) purification of solubility in acid natural collagen: above-mentioned solubility in acid collagen solution is separated with the 14000-16000rpm high speed centrifuge, remove filtering residue, adding 3-5M NaCl in the supernatant saltouts, gained precipitation with the dissolving of 0.5-1M acetic acid, obtains concentration and is not degeneration natural collagen solution of 1% solubility in acid after centrifugalize.
(3) preparation of water-soluble natural collagen: solubility in acid natural collagen solution 200 weight portions of getting above-mentioned purification; add acylating agent 1-10 weight portion, reaction is 3-8 hour under the room temperature, measures the acylation reaction degree; the acidylate rate is 92-95%, obtains the not thick product of degeneration natural collagen of water solublity.
(4) purification of water-soluble natural collagen: with the not thick product centrifugalize of degeneration natural collagen of above-mentioned water solublity, collecting precipitation, reuse 0.01-0.1M diluted acid water washing precipitation 3 times, the precipitation that obtains makes not degeneration natural collagen of water solublity after lyophilization, promptly can be used as raw material biological active collagen of the present invention, its molecular weight can record by electrophoresis analytical method.
Compared with prior art, the invention has the beneficial effects as follows: it is primary raw material that cosmetics of the present invention adopt biological active collagen, have following characteristics: (1) biological active collagen has the right hand triple helix structure identical with the collagen structure of human body skin, fabulous with the human skin compatibility, under the zymolysis of collagenase in human body (collagen hydrolysate enzyme), make it fully by skin absorbs by two approach in extracellular and the cell, nutritional labeling can not run off, and can reach good beauty and skin care effect.(2) by consumer's trial edition invention series of products surplus 100, it is good to show that cosmetics that the present invention contains biological active collagen have a moisture retention, effects such as wrinkle removal, slow down aging, reparation skin, and scalable skin matter, the desalination mottle, promote effects such as Skin Cell growth and breeding, have tangible biological activity; And collagen is considered to the low-down protein of antigenicity usually, has proved also that by on probation cosmetics of the present invention have extremely low antigenicity, and anaphylaxis does not take place, and does not have and repels, and is fit to the crowd of various skin matter, and safety is good.
Description of drawings
Fig. 1 is the primary raw material biological active collagen in the cosmetics of the present invention and the electrophoretic analysis figure of gelatin and hydrolytic collagen.Wherein, a is the standard relative molecular weight, and b is a biological active collagen, and c is a gelatin, and d is a hydrolytic collagen.
The specific embodiment
The present invention is described in further detail below in conjunction with the specific embodiment.
Embodiment 1
The present embodiment cosmetics that the component of following percentage composition is formed of serving as reasons:
Collagen concentration is 0.5% biological active collagen 100%.
Embodiment 2
The present embodiment cosmetics that the component of following percentage composition is formed of serving as reasons:
Collagen concentration is 0.55% biological active collagen 76, Laticauda semifasciata seed extract 4%.
Embodiment 3
The present embodiment cosmetics that the component of following percentage composition is formed of serving as reasons:
Collagen concentration is 0.3% biological active collagen 99.49%, Laticauda semifasciata seed extract 0.01%, glycerol 0.5%.
Embodiment 4
The present embodiment cosmetics that the component of following percentage composition is formed of serving as reasons:
Collagen concentration is 0.5% biological active collagen 88%, Laticauda semifasciata seed extract 2%, glycerol 10%.
Embodiment 5
The present embodiment cosmetics that the component of following percentage composition is formed of serving as reasons:
Collagen concentration is 0.05% biological active collagen 79.9%, glycerol 20%, Estee Lauder essence 0.1%.
More than each embodiment product only need each component is mixed in proportion, gets rid of gas, sterilization promptly according to the conventional method of preparation cosmetics.
Consumer's trial edition invention cosmetics situation is as follows:
Routine number on probation: 20-30 year crowd, women 30 people, male 10 people; 30-45 year crowd, women 30 people, male 10 people; Crowd more than 45 years old, women 30 people, male 10 people.Totally 120 people.
Result on trial: all are on probation to have no adverse reaction per capita, and has following effect: (1) moisture retention is good: product of the present invention uses on skin proves that its dispersibility, absorbability are good, can make the epidermis of skin obtain competent moisture, makes skin have gloss.(2) effect of wrinkle removal, slow down aging, reparation skin is obvious: all triers become tiny at use product rear face wrinkle of the present invention, even disappear; It is delicate, smooth, flexible that skin becomes.(3) scalable skin matter, desalination mottle: after a period of time on probation, all triers' the colour of skin all has improvement in various degree.
Consumer's trial state has fully proved beauty and skin care effect and the safety that cosmetics of the present invention are good.
Claims (6)
1. cosmetics that contain biological active collagen, it contains following components in weight percentage: the plant extract 0-4% of biological active collagen 76-100%, Pear Power effect, water solublity moisturizing agent 0-20%;
Wherein, the material of described biological active collagen for making by the method that may further comprise the steps:
(1) extraction of solubility in acid natural collagen: get fresh porcine skin/Corii Bovis seu Bubali or salted Corii Sus domestica/Corii Bovis seu Bubali 100 weight portions, wash fleshing with water after, add degreasing agent 2-3 part and handled 3-5 hour, coprocessing 2 times, pull out a mao back split, obtain skin corium, soaked 1 day with 10% saline solution, washing cooling back chopping, get onal piece 10 weight portions of chopping, to 2-4, add proteinase-10 .1-1 part with sour adjust pH, in temperature 4-6 ℃ stir process 1-3 days, obtain not degeneration natural collagen solution of solubility in acid;
(2) purification of solubility in acid natural collagen: above-mentioned solubility in acid collagen solution is separated with the 14000-16000rpm high speed centrifuge, remove filtering residue, adding 3-5M NaCl in the supernatant saltouts, gained precipitation with the dissolving of 0.5-1M acetic acid, obtains concentration and is not degeneration natural collagen solution of 1% solubility in acid after centrifugalize;
(3) preparation of water-soluble natural collagen: solubility in acid natural collagen solution 200 weight portions of getting above-mentioned purification, add acylating agent 1-10 weight portion, reaction is 3-8 hour under the room temperature, measures the acylation reaction degree, the acidylate rate is 92-95%, obtains the not thick product of degeneration natural collagen of water solublity;
(4) purification of water-soluble natural collagen: with the not thick product centrifugalize of degeneration natural collagen of above-mentioned water solublity, collecting precipitation, reuse 0.01-0.1M diluted acid water washing precipitation 3 times, the precipitation that obtains makes not degeneration natural collagen of water solublity after lyophilization, promptly can be used as raw material biological active collagen of the present invention;
The molecular weight of biological active collagen is 280-300KD, and collagen concentration is 0.05-0.55%.
2. the cosmetics that contain biological active collagen according to claim 1 is characterized in that: the percentage by weight of described each component is: collagen concentration is the biological active collagen 88-99.49% of 0.05-0.55%, plant extract 0.01-2%, the water solublity moisturizing agent 0.5-10% of Pear Power effect.
3. the cosmetics that contain biological active collagen according to claim 1 is characterized in that: the collagen concentration of described biological active collagen is 0.3-0.5%.
4. the cosmetics that contain biological active collagen according to claim 1 is characterized in that: the plant extract of described Pear Power effect is the Laticauda semifasciata seed extract.
5. the cosmetics that contain biological active collagen according to claim 1 is characterized in that: described water solublity moisturizing agent is a glycerol.
6. the cosmetics that contain biological active collagen according to claim 1 is characterized in that: also contain the used for cosmetic essence of 0.01-0.1% in the described cosmetics, and each component sum is 100% in these cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100211008A CN100441165C (en) | 2006-06-05 | 2006-06-05 | Cosmetics containing biological active collagen |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100211008A CN100441165C (en) | 2006-06-05 | 2006-06-05 | Cosmetics containing biological active collagen |
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| CN1895205A CN1895205A (en) | 2007-01-17 |
| CN100441165C true CN100441165C (en) | 2008-12-10 |
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| CNB2006100211008A Active CN100441165C (en) | 2006-06-05 | 2006-06-05 | Cosmetics containing biological active collagen |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1351083A (en) * | 2000-11-01 | 2002-05-29 | 四川大学 | Process for preparing collagen |
| CN1470640A (en) * | 2003-06-25 | 2004-01-28 | 暨南大学 | Collagenic protein powder, and its preparing method and use |
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2006
- 2006-06-05 CN CNB2006100211008A patent/CN100441165C/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1351083A (en) * | 2000-11-01 | 2002-05-29 | 四川大学 | Process for preparing collagen |
| CN1470640A (en) * | 2003-06-25 | 2004-01-28 | 暨南大学 | Collagenic protein powder, and its preparing method and use |
Non-Patent Citations (2)
| Title |
|---|
| 酶法提取生猪皮胶原工艺条件的研究. 施辉阳.食品工业科技，第7期. 2004 |
| 酶法提取生猪皮胶原工艺条件的研究. 施辉阳.食品工业科技，第7期. 2004 * |
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| CN1895205A (en) | 2007-01-17 |
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Owner name: CHENGDU KELUO RUISHI COSMETICS CO., LTD. Free format text: FORMER OWNER: YANG XINCHENG Effective date: 20070323 |
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Effective date of registration: 20070323 Address after: High tech Zone Chengdu city Sichuan province 610041 Gaopeng Road No. 2 Building 2 Conte incubator Applicant after: Chengdu Ke Shi Rui Shi Cosmetics Co., Ltd. Address before: 615000 Sichuan city of Chengdu province Wuhou District Shaoling Street No. 1 Building 2 unit 13 No. 4 Applicant before: Yang Xincheng |
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Effective date of registration: 20211103 Address after: No. 1239, middle section of Yizhou Avenue, Chengdu hi tech Zone, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan 610041 Patentee after: CHENGDU QINGKE BIO-TECHNOLOGY Co.,Ltd. Address before: High tech Zone Chengdu city Sichuan province 610041 Gaopeng Road No. 2 Building 2 Conte incubator Patentee before: CHENGDU CORRINE COSMETICS CO.,LTD. |