CN100434901C

CN100434901C - Teaching kit for hemoglobin gel chromatography

Info

Publication number: CN100434901C
Application number: CNB2006100232466A
Authority: CN
Inventors: 曹阳; 张霞; 祝新德; 陈熹兮; 张承康; 熊顺子
Original assignee: Shanghai Jiaotong University
Current assignee: Shanghai Jiaotong University
Priority date: 2006-01-12
Filing date: 2006-01-12
Publication date: 2008-11-19
Anticipated expiration: 2026-01-12
Also published as: CN1804600A

Abstract

The present invention relates to a teaching kit for hemoglobin gel chromatography. Aiming at a hemoglobin gel chromatography experiment, reagents and material are carried out with optimizing configuration according to ten groups with one experiment teaching standard class. The present invention comprises 0.02 M of phosphate buffer with pH7.0, sampling phosphate buffer, reduction type ferrous salt, potassium ferricyanide phosphoric acid buffer, sterile water, an EDTA solution, sampling centrifuge tubes with different colors, sampling dropping pipettes, filter paper sheets, liquid division dropping pipettes for teachers, etc. A service manual is prepared in each kit which can be used for a standard class to experiment. The present invention can be applied to bioscience experiment introduction teaching, and can save experiment preparation time for teachers and reduce the configuration of experimenters and the technology difficulty of experiment preparation. A hemoglobin gel chromatography experiment can be quickly completed with high efficiency, and good experiment effect can be obtained.

Description

The teaching kit of hemoglobin gel chromatography

Technical field

The present invention relates to a kind of teaching kit of hemoglobin gel chromatography, be used for the experiment of biological scientific experiment introduction teaching hemoglobin gel chromatography.

Background technology

At present, China's biological scientific experiment teaching faces to have high input, produce imitates low awkward situation, and general laboratory all needs to dispose simultaneously experiment teacher and laboratory technician.Before experiment, need to do a large amount of preliminary work, often will buy earlier and test relevant plurality of reagents and material, the preparation of dividing into groups of the number situation of experimental then requirement, experiment.Experimenter's configuration that the finishing of these work needs some, reagent of Gou Maiing and material be often all greater than the consumption of actual needs simultaneously, thereby existence waste in various degree, improved experimental cost.The technical difficulty of testing preliminary work simultaneously is also very high, and to the use amount of each experiment group, to the accurate tolerance and the distribution of every kind of reagent, every kind of material usage, it is careful accurate all to require, to guarantee normally carrying out of experiment.This shows that preparing experiment need consume and waste great amount of manpower and material resources, financial resources, be badly in need of a kind of mode of new preparing experiment.Though it is very general that domestic and imported scientific research kit is used in China's bio-science research, have convenient, fast, efficient and result's characteristics accurately and reliably, but do not see the kit patented product that is applicable to the teaching of biological scientific experiment introduction so far at home as yet.Because the scientific research kit generally all at a certain particular experiment link, has very strong technical characterictic, its content is difficult to the education experiment on the adaptive surface simultaneously.Thereby, invent a kind of teaching kit of biological scientific experiment teaching that is applicable to and seem particularly necessary and urgent.

Summary of the invention

The objective of the invention is at the deficiencies in the prior art and actual needs, a kind of teaching kit that is applicable to hemoglobin gel chromatography is provided, can directly be used for finishing education experiment easily, guarantee the high-level efficiency of experiment, save experimental cost.

For realizing such purpose, the present invention is directed to the hemoglobin gel chromatography experiment, reagent and material are contained in the kit, and be optimized configuration by 10 groups one experimental teaching standard class, material in the kit comprises phosphate buffer, goes up sample phosphate buffer, reduced form ferrous salt, potassium ferricyanide phosphate buffer solution, sterilized water, EDTA (ethylenediamine tetraacetic acid) sodium salt solution (0.2M Na according to the use amount design of 10 experiment groups ₂HPO ₄, 80mM Na ₂H ₂EDTA), sample centrifuge tube, last sample dropper, filter paper and teacher use separatory dropper etc. on three looks.Be equipped with service manual in each kit, can carry out experiment for a standard class.

Teaching kit of the present invention contains a complete set of material and the reagent of hemoglobin gel chromatography experiment.Be equipped with material by 10 experiment group use amounts in the kit, the consumption of reagent and material is through the experimental teaching optimal value groping to obtain repeatedly over the years, comprise that one bottle of phosphoric acid buffer liquid formulation (is used to prepare pH7.0, phosphoric acid concentration is the 2000ml phosphate buffer of 0.02M, every set of dispense 200ml is used for the abundant wash-out of sample), go up sample phosphate buffer (pH7.0, concentration 0.02M for one bottle, 20ml), one bottle of reduced form ferrous salt (solid FeSO ₄7H ₂O, 44.5mg dress within it, during experiment now with now being dissolved into solution in order to avoid oxidation), one bottle of potassium ferricyanide phosphate buffer solution (4ml), one bottle of sterilized water (4ml) (being used for dissolving and reducing type ferrous salt), one bottle of EDTA sodium salt solution (concentration 0.2M, 4ml), one wraps sample centrifuge tube (specification 1.5ml, respectively 10 of pink, white, yellow centrifuge tubes), and one wraps sample dropper (10), one bag filter paper (10), a teacher uses separatory dropper and a kit service manual.

One group of material can use for one group of student (2-3 people) in the kit of the present invention, and ten groups of materials can be carried out experiment for a standard class (30 people).Can also be equipped with corresponding experiment instruction teaching material and CD according to different needs.

Before the experiment, experiment teacher or preparation personnel only need do simple preliminary work: use the teacher will go up the sample phosphate buffer and be sub-packed in the sample centrifuge tube white pipe with the separatory dropper, and every pipe 0.7ml, totally 10 pipes are managed for every group 1.Open the ferrous saltcellar of reduced form, take out the ferrous salt cartridge bag, ferrous salt is poured in the former bottle, with one bottle of sterilized water being furnished with in the kit all to going into to dissolve molysite in the bottle, treat molysite dissolve fully after with another bottle EDTA sodium salt solution in the kit all to going into mixing in the bottle, be mixed with sample reduced iron solution.Be sub-packed in the yellow pipe of sample centrifuge tube with the reduced iron solution of separatory dropper with the teacher preparation, every pipe 0.4ml, totally 10 pipes are managed for every group 1.1ml anti-freezing blood sample is dissolved in the 4ml potassium ferricyanide phosphate buffer solution, is made into the upper prop blood sample, with the separatory dropper upper prop blood sample is sub-packed in the red pipe of sample centrifuge tube with the teacher, every pipe 0.5ml, totally 10 pipes are managed for every group 1.

During experiment, the student only needs chromatographic column to be assembled (also can be installed by teacher in advance) by teacher's explanation and put filter paper on the post bed surface, washed the post bed 20 minutes with the prepared phosphoric acid damping fluid, then with every component to three kinds of color centrifuge tubes use the sample dropper and carefully be added drop-wise to the sample in the centrifuge tube in the post by yellow, white, red order, use the phosphate buffer wash-out again, promptly can be observed tangible experimental phenomena.Treat at last to continue to use the phosphate buffer wash-out after coloring matter flows out pillar fully, can finish experiment when this damping fluid is about to use up.

The principle that teaching kit of the present invention has adopted gel that biomacromolecule is separated, also utilized haemoglobin oxidation reduction change in color experimental phenomena intuitively interesting, experimental result is cheer and bright, the experiment teacher no longer needs the weighing of medicine, these loaded down with trivial details experiments of measuring of solution to prepare just can finish the student experimenting teaching, reduced experimenter's configuration so on the one hand, also make experimental teaching quick, efficient on the other hand, the student can obtain standard of comparison, reliable experimental result.

The present invention is optimized configuration with experiment reagent and material by 10 groups one experimental teaching standard class for the teaching of biological scientific experiment introduction provides a kind of economical and practical teaching kit, has avoided unnecessary waste, has saved reagent and material.During experiment, after the teacher reads the kit service manual, need only according to explanation every group of required material and reagent are distributed to each group in conjunction with explaining the teaching that can experimentize, saved loaded down with trivial details experiment and prepared the time and efforts of having saved the teacher.Simultaneously, the raising of work efficiency makes experimenter's configuration that certain minimizing can be arranged.The service manual that the student has according to teacher's classroom explanation and kit can have good grasp and finish experiment smoothly experiment content in the short period of time.Kit of the present invention makes the hemoglobin gel chromatography experiment can finish and obtain good experiment effect efficiently, fast, and financial resources, material resources, the manpower that experiment can be prepared required consumption drop to minimum.

Description of drawings

Fig. 1 is the reagent that kit of the present invention comprised and the displaying of medicine.

Among Fig. 1,1. kit, 2. phosphoric acid buffer liquid formulation, 3. go up the sample phosphate buffer, 4. reduced form ferrous salt, 5. potassium ferricyanide phosphate buffer solution, 6. sterilized water, 7.EDTA sodium salt solution, 8. go up the sample centrifuge tube, 9. go up the sample dropper, 10. the teacher uses the separatory dropper, 11. filter paper, 12. kit service manuals.

Embodiment

Below in conjunction with accompanying drawing technical scheme of the present invention is further described.

Fig. 1 is an embodiment of teaching kit provided by the invention, has comprised required a complete set of material and the reagent of hemoglobin gel chromatography experiment in the kit.As shown in Figure 1, experiment reagent and material are optimized configuration by 10 groups one experimental teaching standard class, comprise in the kit 1 that one bottle of phosphoric acid buffer liquid formulation 2, one bottle go up the EDTA sodium salt solution 7, of sample phosphate buffer 3, one bottle of reduced form ferrous salt 4, one bottle of 4ml potassium ferricyanide phosphate buffer solution 5, one bottle of 4ml sterilized water 6 (being used for dissolving and reducing type ferrous salt), one bottle of 4ml concentration 0.2M and wrap sample centrifuge tube 8,10 of bags and go up sample dropper 9, teacher with separatory dropper 1O, 10 filter papers 11 of a bag and kit service manual 12.Wherein said phosphoric acid buffer liquid formulation 2 can be prepared 2000ml for solid mixing medicine, and pH7.0, phosphoric acid concentration are the phosphate buffer of 0.02M, and every set of dispense 200ml is used for the abundant wash-out of sample.Described upward sample phosphate buffer 3 is pH7.0, concentration 0.02M, 20ml, and described reduced form ferrous salt 4 is solid FeSO ₄7H ₂O, 44.5mg adorns within it, during experiment now with now being dissolved into solution in order to avoid oxidation.Described upward sample centrifuge tube 8 specifications are 1.5ml, respectively 10 of pink, white, yellow centrifuge tubes.

In the kit in above-mentioned each inner wrapping the consumption of material be the optimal value that experimental teaching over the years obtains, one group of material uses for one group of student (2-3 people), ten groups of materials can be carried out experiment for a standard class (30 people).

During experiment, each experiment group is assigned in every group of material taking-up in the kit, according to the explanation of service manual, the teacher explains and can finish experiment by instruction of papil.In addition, can also dispose corresponding experiments supporting in the kit according to different needs and instruct teaching material and CD, combining with teaching experimentizes, better effects if.

Claims

1, a kind of teaching kit of hemoglobin gel chromatography, it is characterized in that hemoglobin gel chromatography is tested required reagent and material is optimized configuration by 10 groups one experimental teaching standard class, comprise one bottle of phosphoric acid buffer liquid formulation (2), go up sample phosphate buffer (3) for one bottle, one bottle of reduced form ferrous salt (4), one bottle of 4ml potassium ferricyanide phosphate buffer solution (5), one bottle of 4ml sterilized water (6), the disodium edta solution (7) of one bottle of 4ml concentration 0.2M, one wraps sample centrifuge tube (8), 10 of one bags are gone up sample dropper (9), a teacher uses separatory dropper (10), one bag 10 filter papers (11) and kit service manual (12); Wherein said phosphoric acid buffer liquid formulation (2) is used to prepare 2000ml, pH7.0, phosphoric acid concentration is the phosphate buffer of 0.02M; Described upward sample phosphate buffer (3) is pH7.0, concentration 0.02M, 20ml; Described reduced form ferrous salt (4) is solid FeSO ₄7H ₂O, 44.5mg; Described upward sample centrifuge tube (8) specification is 1.5ml, respectively 10 of pink, white, yellow centrifuge tubes.

2, according to the teaching kit of the hemoglobin gel chromatography of claim 1, it is characterized in that also being equipped with experiment instruction teaching material and CD in the kit (1).