CN206052021U - A kind of PCR buffer kits - Google Patents

Abstract

The utility model discloses a kind of PCR buffer kits, which includes box body and covers the lid on the box body；Upper cartridge body that the box body includes sequentially arranging from top to bottom, middle box body, lower box body；The sample cell of at least four Reagent Tubes for filling reagent and a testing sample is embedded with the middle box body, the bottom of the every Reagent Tube and sample cell is all respectively equipped with the passage connected with lower box body, and check valve is designed with each passage, the control unit of each check valve is respectively positioned on the outer wall of middle box body；The drive mechanism matched with the Reagent Tube and sample cell on middle box body is provided with the upper cartridge body；The lower box body is provided with gene chip layer, and the gene chip layer is connected with aforementioned agents pipe and aforementioned sample pipe respectively by aforementioned channels.This utility model can reduce reagent consumption, it is easy to accomplish automatization and portability, beneficial to the reduction of testing cost.

Description

A kind of PCR buffer kits

Technical field

This utility model is related to DNA detection analysis technical field, more particularly to one kind can reduce reagent consumption, it is easy to real Existing automatization and portability, beneficial to the PCR buffer kits of the reduction of testing cost.

Background technology

When DNA in biological sample is analyzed, often needs to carry out DNA extraction, DNA cloning (PCR amplifications) and expand Increase the steps such as detection (electrophoresis detection or hybridization check).Laboratory routine DNA extraction method is also mainly manual test kit at present, Which needs the equipment such as centrifuge, and operating procedure is relatively time-consuming.

DNA extraction kit is welcomed by the people using starting in recent years, and which includes using solid phase adsorption (solid-phase adsorption), protein and DNA phase postprecipitations (sequential protein and DNA Precipitation), the method such as micro- magnetic bead absorption (magnetic bead adsorption).Although also occurring in that very at present Many automatic modes, but large-scale automation equipment is required to, and it is not good for the corpse or other object for laboratory examination and chemical testing extraction effect of small volume.Additionally, will After DNA extraction is complete, the detection of its back segment, such as polymerase chain reaction (polymerase chain reaction, PCR), more It is time and effort consuming.

Therefore traditional method or test kit at this stage all consume more to reagent, rely on high to equipment, detect into This is also higher；Especially infectious disease is detected, due to higher pathogenic and infectiousness so that disposable detection becomes must Will, and the method cost of routine is all higher, and equipment cannot single use so that pollution and the chance for infecting increase.

The content of the invention

In order to solve the problems of the prior art, the purpose of this utility model is to provide one kind and can reduce reagent consumption, Easy to automate and portability, beneficial to the PCR buffer kits that testing cost is reduced.

To achieve these goals, the technical solution adopted in the utility model is：

A kind of PCR buffer kits include box body and cover the lid on the box body；The box body is included from upper And under sequentially arrange upper cartridge body, middle box body, lower box body；Be embedded with the middle box body at least four Reagent Tubes for filling reagent and The bottom of the sample cell of a piece testing sample, the every Reagent Tube and sample cell is all respectively equipped with connect with lower box body logical Check valve is designed with road, and each passage, the control unit of each check valve is respectively positioned on the outer wall of middle box body；The upper box The drive mechanism matched with the Reagent Tube and sample cell on middle box body is provided with body；The lower box body is provided with gene chip Layer, the gene chip layer are connected with aforementioned agents pipe and aforementioned sample pipe respectively by aforementioned channels.

Preferred technical scheme, the Reagent Tube are four, and are respectively the PCR buffering agents for filling PCR buffer Manage, fill the dNTPs Reagent Tubes of dNTPs liquid (dideoxyribonucleotide triphosphate), fill the polymerase Reagent Tube of polymerase, fill The primed probe mixed liquor Reagent Tube of primed probe mixed liquor；Four Reagent Tubes and sample cell are " one " word linear array.

Preferred technical scheme, the drive mechanism are cylinder of the cylinder diameter less than six millimeters.

Preferred technical scheme, the corresponding PCR buffering agents pipe of the gene chip layer, dNTPs Reagent Tubes, polymerization Enzymatic reagent pipe, primed probe mixed liquor Reagent Tube, sample cell be provided with matching receiving PCR buffer PCR buffering liquid zone, The dNTPs liquid zones for accommodating dNTPs liquid, the polymerase area for accommodating polymerase, the primed probe mixing for accommodating primed probe mixed liquor Liquid zone, sample liquid zone；The PCR bufferings liquid zone, dNTPs liquid zones, polymerase area, primed probe mixing liquid zone, sample liquid zone lead to Cross aforementioned channels respectively with PCR buffering agents pipes, dNTPs Reagent Tubes, polymerase Reagent Tube, primed probe mixed liquor reagent The bottom connection of pipe, sample cell；Fluid channel is provided with the gene chip layer, primed probe mixing liquid zone passes through fluid channel After connecting with polymerase area, connected with dNTPs liquid zones by fluid channel, then liquid zone is buffered with PCR by fluid channel and connected, finally Connected with sample liquid zone by fluid channel.

Preferred technical scheme, the fluid channel are provided with " S " bend.The bend of setting is conducive to the flat of flow rate of liquid Surely.

Preferred technical scheme, buffers in the PCR and sets between the connectivity part and the connectivity part of the sample liquid zone of liquid zone There is PCR reagent buffer area.The PCR reagent buffer area of setting is conducive to going again to participate in after the mix homogeneously of PCR reagent liquid is abundant The detection reaction of sample liquid.

Preferred technical scheme, the fluid channel end after the connectivity part of the sample liquid zone is provided with sample detection zone.

By using above technical scheme, a kind of PCR buffer kits of this utility model compared with prior art, its because It is to adopt biochip technology, and is monoblock type scheme, therefore be convenient for carrying, while reagent consumption can be reduced, it is easy to accomplish Automatization, is conducive to the reduction of testing cost.

Description of the drawings

Fig. 1 is a kind of schematic perspective view of PCR buffer kits of this utility model；

Fig. 2 is a kind of cross-sectional schematic of PCR buffer kits of this utility model；

Fig. 3 is a kind of structural representation of the gene chip layer of PCR buffer kits of this utility model.

Specific embodiment

To make the purpose of this utility model, technical scheme and advantage of greater clarity, with reference to instantiation, to this Utility model is further described.It should be understood that these descriptions are simply exemplary, and it is not intended to limit of the present utility model Scope.Additionally, in the following description, the description to known features and technology is eliminated, it is practical to avoid unnecessarily obscuring New concept.

As shown in Figure 1 to Figure 3, a kind of PCR buffer kits of the invention include box body 1 and cover on the box body 1 Lid 2；Upper cartridge body 11 that the box body 1 includes sequentially arranging from top to bottom, middle box body 12, lower box body 13；The middle box body At least four Reagent Tubes 3 and the sample cell 4 of a testing sample for filling reagent, the every Reagent Tube 3 and sample are embedded with 12 The bottom of QC 4 is all respectively equipped with the passage 5 connected with lower box body 13, and each passage 5 and is designed with check valve 6, each list It is respectively positioned on the outer wall of middle box body 12 to the control unit 7 of valve 6；It is provided with the upper cartridge body 11 and the reagent on middle box body 12 The drive mechanism 8 that pipe 3 and sample cell 4 match；The lower box body 13 is provided with gene chip layer 9, and the gene chip layer 9 passes through Aforementioned channels 5 are connected with aforementioned agents pipe 3 and aforementioned sample pipe 4 respectively.

The Reagent Tube 3 is four, and respectively fills the PCR buffering agents pipe 31 of PCR buffer, fills dNTPs The dNTPs Reagent Tubes 32 of liquid, the polymerase Reagent Tube 33 for filling polymerase, the primed probe mixing for filling primed probe mixed liquor Liquid Reagent Tube 34；Four Reagent Tubes (31,32,33,34) and sample cell 4 are " one " word linear array.

The drive mechanism 8 is cylinder of the cylinder diameter less than six millimeters.

The corresponding PCR buffering agents pipe 31, dNTPs Reagent Tubes 32 of the gene chip layer 9, polymerase Reagent Tube 33rd, primed probe mixed liquor Reagent Tube 34, sample cell 4 be provided with matching receiving PCR buffer PCR buffering liquid zone 91, The dNTPs liquid zones 92 for accommodating dNTPs liquid, the polymerase area 93 for accommodating polymerase, the primed probe for accommodating primed probe mixed liquor Mixing liquid zone 94, sample liquid zone 95；The PCR bufferings liquid zone 91, dNTPs liquid zones 92, polymerase area 93, primed probe mixed liquor Area 94, sample liquid zone 95 are by aforementioned channels 5 respectively with PCR buffering agents pipe 31, dNTPs Reagent Tubes 32, be polymerized enzymatic reagent The bottom connection of pipe 33, primed probe mixed liquor Reagent Tube 34, sample cell 4；Fluid channel is provided with the gene chip layer 9 10, primed probe mixing liquid zone 94 after fluid channel 10 is connected with polymerase area 93, by fluid channel 10 and dNTPs liquid zones 92 Connection, then connected with PCR bufferings liquid zone 91 by fluid channel 10, connect with sample liquid zone 95 finally by fluid channel 10.

The fluid channel 10 is provided with " S " bend.

PCR reagent is provided between the connectivity part and the connectivity part of the sample liquid zone 95 of PCR bufferings liquid zone 91 to delay Deposit area 96.

10 end of fluid channel after the connectivity part of the sample liquid zone 95 is provided with sample detection zone 97.

The course of work of the present utility model is：

When needing to detect analyte sample fluid, lid 2 is opened, upper cartridge body 11 is removed, in the sample cell 4 of middle box body 12 Add analyte sample fluid.Upper cartridge body 11 is installed again, check valve 6 is all opened, start drive mechanism 8, by treating in sample cell 4 PCR buffer, the dNTPs liquid in dNTPs Reagent Tubes 32 in survey sample liquid, PCR buffering agents pipe 31, polymerase Reagent Tube Primed probe mixed liquor in 33 polymerase, primed probe mixed liquor Reagent Tube 34 is driven to gene chip layer 9 respectively In sample liquid zone 95, in PCR bufferings liquid zone 91, in dNTPs liquid zones 92, in polymerase area 93, in primed probe mixing liquid zone 94； Primed probe mixed liquor Jing fluid channels 10 on gene chip floor 9 in primed probe mixing liquid zone 94 are first and in polymerase area 93 Polymerase mixes, then Jing fluid channels 10 mix with the dNTPs liquid in dNTPs liquid zones 92, most after Jing fluid channels 10 and PCR buffer PCR buffer in area 91 is mixed to form PCR reagent, and PCR reagent Jing fluid channel 10 flows into PCR reagent buffer area 96, and in PCR Stable PCR reagent is formed in reagent buffer area 96.PCR reagent Jing fluid channel 10 is mixed with the sample liquid in sample liquid zone 95, And Jing fluid channels 10 flow into sample detection zone 97 and are reacted.Sample detection zone 97 is arranged on the end of fluid channel 10, question response After fully, check valve 6 is closed, lower box body 13 is removed, you can the reaction result of immediate observation sample detection zone 97.Entirely reacted Journey is carried out in confined space, has been prevented the pollutant in the external world, has been also beneficial to the healthy of testing staff.

Above-mentioned specific embodiment is exemplary, is to preferably make skilled artisans appreciate that originally Patent, it is impossible to be not understood as including this patent the restriction of scope；As long as according to disclosed in this patent spirit made appoint How with change or modification, each fall within the scope that this patent includes.

Claims (7)

1. a kind of PCR buffer kits, which includes box body and covers the lid on the box body；Characterized in that, described Upper cartridge body that box body includes sequentially arranging from top to bottom, middle box body, lower box body；At least four are embedded with the middle box body and fill examination The bottom of the sample cell of the Reagent Tube of agent and a testing sample, the every Reagent Tube and sample cell is all respectively equipped with and lower box The passage of body connection, and check valve is designed with each passage, the control unit of each check valve is respectively positioned on the outer wall of middle box body On；The drive mechanism matched with the Reagent Tube and sample cell on middle box body is provided with the upper cartridge body；On the lower box body Gene chip layer is provided with, the gene chip layer is connected with aforementioned agents pipe and aforementioned sample pipe respectively by aforementioned channels.

2. a kind of PCR buffer kits according to claim 1, it is characterised in that the Reagent Tube is four, and point The PCR buffering agents pipes of PCR buffer Wei not be filled, the dNTPs Reagent Tubes of dNTPs liquid is filled, is filled the polymerization of polymerase Enzymatic reagent pipe, the primed probe mixed liquor Reagent Tube for filling primed probe mixed liquor；Four Reagent Tubes and sample cell are " one " Word linear array.

3. a kind of PCR buffer kits according to claim 2, it is characterised in that the drive mechanism is that cylinder diameter is little In six millimeters of cylinder.

4. a kind of PCR buffer kits according to claim 3, it is characterised in that the gene chip layer correspondence institute State PCR buffering agents pipes, dNTPs Reagent Tubes, polymerase Reagent Tube, primed probe mixed liquor Reagent Tube, sample cell be provided with Matching receiving PCR buffer PCR buffering liquid zone, accommodate dNTPs liquid dNTPs liquid zones, accommodate polymerase polymerase Area, the primed probe mixing liquid zone for accommodating primed probe mixed liquor, sample liquid zone；The PCR buffers liquid zone, dNTPs liquid zones, gathers Synthase area, primed probe mixing liquid zone, sample liquid zone by aforementioned channels respectively with PCR buffering agents pipes, dNTPs reagents Pipe, polymerase Reagent Tube, primed probe mixed liquor Reagent Tube, the bottom connection of sample cell；Arrange on the gene chip layer There is fluid channel, primed probe mixing liquid zone is connected with dNTPs liquid zones by fluid channel after fluid channel is connected with polymerase area It is logical, then connected with PCR bufferings liquid zone by fluid channel, connect with sample liquid zone finally by fluid channel.

5. a kind of PCR buffer kits according to claim 4, it is characterised in that it is curved that the fluid channel is provided with " S " Road.

6. a kind of PCR buffer kits according to claim 5, it is characterised in that buffer the company of liquid zone in the PCR PCR reagent buffer area is provided between the connectivity part of logical place and the sample liquid zone.

7. a kind of PCR buffer kits according to claim 6, it is characterised in that in the connection of the sample liquid zone Fluid channel end after place is provided with sample detection zone.