CN100339489C - Teaching kit of DNA fingerprint map analyzing - Google Patents

Abstract

The present invention relates to a teaching kit for DNA fingerprint map analysis, which comprises a TAE electrophoresis buffer concentrated solution, agarose, a six-color subpackage centrifuge tube, a blue sample adding centrifuge tube, a DNA sample adding buffer solution, ethidium bromide, six DNA samples and a kit service manual, wherein the six-color subpackage centrifuge tube can be used for the subpackage of the DNA samples; the blue sample adding centrifuge tube can be used for the subpackage of the DNA sample adding buffer solution; the ethidium bromide is circular filter paper which is adsorbed with the concentrated ethidium bromide solution and dried, and is stored in the centrifuge tube and wrapped by tin foil for protecting from light; the DNA samples are six prepared DNA samples which have different enzyme cutting electrophoretic bands. Reagents and material can be carried out optimizing configuration according to ten groups with one experiment teaching standard class. The present invention can be applied to bioscience experiment introduction teaching, and can largely reduce the fussy preparation work before an experiment is carried out, and reduce the technology difficulty of experiment preparation. The experiment can be quickly completed in high efficiency, and favorable effect can be obtained.

Description

The teaching kit that dna fingerprinting is analyzed

Technical field

The present invention relates to the teaching kit of a kind of dna fingerprinting analysis (DNA fingerprinting analysis), be used for biological scientific experiment introduction teaching dna fingerprinting analysis experiment.

Background technology

At present, China's biological scientific experiment teaching faces to have high input, produce imitates low awkward situation, and general laboratory all needs to dispose simultaneously experiment teacher and laboratory technician.Before experiment, need to do a large amount of preparation work, often will buy earlier and test relevant plurality of reagents and material, the preparation of dividing into groups of the number situation of experimental then requirement, experiment.Experimenter's configuration that the finishing of these work needs some amount, reagent of Gou Maiing and material be often all greater than the consumption of actual needs simultaneously, thereby existence waste in various degree, improved experimental cost.The technical difficulty of testing preparation work simultaneously is also very high, and to the usage quantity of each experiment group, to the accurate tolerance and the distribution of every kind of reagent, every kind of material usage, it is careful accurate all to require, to guarantee normally carrying out of experiment.This shows that preparing experiment need consume and waste great amount of manpower and material resources, financial resources, be badly in need of a kind of mode of new preparing experiment.Though it is very general that domestic and imported scientific research test kit is used in China's bio-science research, have convenient, fast, efficient and result's characteristics accurately and reliably, but do not see the test kit patented product that is applicable to the teaching of biological scientific experiment introduction so far at home as yet.Because the scientific research test kit generally all at a certain particular experiment link, has very strong technical characterictic, its content is difficult to the education experiment on the adaptive surface simultaneously.Thereby, invent a kind of teaching kit of biological scientific experiment teaching that is applicable to and seem particularly necessary and urgent.The dna fingerprinting analytical technology be modern age life science just produced soon emerging technology, this technology is introduced experimental teaching can make student's forward position development of learning and mastering life science timely, student's cultivation is significant.

Summary of the invention

The objective of the invention is at the deficiencies in the prior art and actual needs, a kind of teaching kit that is applicable to that dna fingerprinting is analyzed is provided, can directly be used for finishing education experiment simply and easily, guarantee the high-efficiency and low-cost of experimental teaching.

For realizing such purpose, the present invention is directed to the dna fingerprinting experiment, reagent and material are contained in the test kit, simplify experiment teacher preparation work before the class as far as possible, and be optimized configuration by 10 groups one experimental teaching standard class.Material in the test kit is according to the usage quantity design of 10 experiment groups, comprise six the DNA samples, DNA sample pipetting volume damping fluid, ethidium bromide (the ethidium bromide that have prepared, EB), Trisaminomethane ethylenediamine tetraacetic acid (EDTA) acetic acid electrophoretic buffer (tris-acetate EDTA, TAE), the 1.5ml centrifuge tube of agarose (Agarose), seven kinds of different colours etc.Be equipped with service manual in each test kit, can carry out experiment for a standard class.

Teaching kit of the present invention contains a complete set of material and the reagent of dna fingerprinting analysis experiment.Lay material by 10 experiment group usage quantitys in the test kit, the consumption of reagent and material is through the experimental teaching optimal value groping to obtain repeatedly over the years, wherein comprise each the 120 μ l of six DNA samples that prepared, comprise 5 suspect S with different restriction enzyme digestion and electrophoresis bands of a spectrum ₁, S ₂, S ₃, S ₄, S ₅Sample and crime scene CS (crime scene, the crime scene) sample, DNA sample pipetting volume damping fluid 200 μ l (6 times of concentrated solutions), TAE electrophoretic buffer 60ml (50 times of concentrated solutions), agarose 3.2g (calculating by every clotting glue 40ml amount), seven looks are used for being distributed to each 10 of each 1.5ml centrifuge tubes of organizing behind packing sample and the sample loading buffer; Consider that the toxicity ethidium bromide carries the inconvenience of storage, the present invention uses disk filter paper to adsorb spissated ethidium bromide solution, be stored in after the drying in the white 5ml centrifuge tube, and with masking foil parcel with lucifuge, reduced the potentially dangerous when this strong carcinogenic medicine is prepared the experiment teacher with former medicinal powder end or solution state as far as possible.This test kit needs-20 ℃ of freezing preservations in addition, to prevent the degraded of sample and reagent, guarantees experiment effect; Be equipped with the test kit service manual in the agent box in addition.

One group of material can use for one group of student (2-3 people) in the test kit of the present invention, and ten groups of materials can be carried out experiment for a standard class (30 people).Can also be equipped with corresponding experiment instruction teaching material and CD according to different needs.

Before the experiment, experiment teacher or preparation personnel only need do simple preparation work by the experiment instruction handbook: the DNA sample in six kinds of different colours centrifuge tubes is installed to (every kind of color 10 pipes of each 10 μ l in the six look centrifuge tubes of being furnished with in the test kit by the color branch of correspondence, totally 60 manage), the sample loading buffer branch installs to every pipe 20 μ l in 10 blue centrifuge tubes, 50 * TAE electrophoretic buffer is become 1 * TAE electrophoretic buffer with distilled water diluting, in 2500ml to 10 250ml reagent bottle of packing, more than divide the material and the medicine that install to be distributed to 10 groups; In indicating the white 5ml centrifuge tube of ethidium bromide, add 0.5ml distilled water, fully be prepared into ethidium bromide solution after the dissolving; Other gets and adds the 3.2g agarose in 400ml1 * TAE electrophoretic buffer, add the ethidium bromide solution that has prepared during heat fused postcooling to the 60 ℃ left and right sides, mixing is poured 10 off-the-shelf glue holders into, put comb well, treat that it solidifies the every component in back and sends out a clotting glue and promptly finished experiment and prepare.

During experiment, the student at first carefully removes the comb and the scotch tape of gel, and gel is put into electrophoresis chamber, adds electrophoretic buffer; Get enzyme and cut the DNA sample, add 1/5 volume (2 μ l) sample buffer, fully mixing; Get 10 μ l samples and add the point sample hole carefully; Opening power and regulating voltage carry out electrophoresis; After electrophoresis was good, powered-down took out gel, observed the migration position of DNA under ultraviolet lamp, analyzes collection of illustrative plates experimental result is discussed.Judge that CS sample and which suspect DNA sample are same samples, find out criminal.The teacher also can allow student oneself prepare sepharose according to practical situation.

Teaching kit of the present invention provides 5 suspicion of crime human sample and crime scene samples with different restriction enzyme digestion and electrophoresis bands of a spectrum that prepared, experimental result is cheer and bright, make to greatest extent that the experiment teacher has saved conversion in the DNA specimen preparation, cultivation, DNA extraction, enzyme is cut and medicine weighing etc. and loaded down with trivial details preparation work thereof, make the experiment preparation simple, quick, efficient, and can the high-quality experimental result that guarantees the student.

The present invention is optimized configuration with experiment reagent and material by 10 groups one experimental teaching standard class for the teaching of biological scientific experiment introduction provides a kind of economical and practical teaching kit, has avoided unnecessary waste, has saved reagent and material.During experiment, after the teacher reads the test kit service manual, as long as carry out preparation simply before the class and every group of required material and reagent be distributed to each group in conjunction with explaining the teaching that can experimentize according to explanation, saved loaded down with trivial details experiment preparation, the time and efforts of having saved the teacher.Simultaneously, the raising of working efficiency makes experimenter's configuration that certain minimizing can be arranged.The service manual that the student has according to teacher's classroom explanation and test kit can have good grasp to experiment content and finish experiment smoothly to obtain the ideal experimental result in the short period of time.Test kit of the present invention makes the dna fingerprinting experiment can finish and obtain good experiment effect efficiently, fast, and financial resources, material resources, the manpower that experiment can be prepared required consumption drop to minimum.

Description of drawings

Fig. 1 is the reagent that test kit of the present invention comprised and the displaying of medicine.

Among the figure, 1 is test kit, and 2 is the TAE electrophoretic buffer, and 3 is agarose, and 4 is six look packing centrifuge tubes, and 5 is blue application of sample centrifuge tube, and 6 is DNA sample pipetting volume damping fluid, and 7 is ethidium bromide, and 8 is six kinds of DNA samples, and 9 is the test kit service manual.

Embodiment

Below in conjunction with accompanying drawing technical scheme of the present invention is further described.

Fig. 1 is an embodiment of teaching kit provided by the invention, has comprised required a complete set of material and the reagent of dna fingerprinting experiment in the test kit.As shown in Figure 1, experiment reagent and material are optimized configuration by 10 groups one experimental teaching standard class, comprise TAE electrophoretic buffer 2 in the test kit 1, agarose 3, six look packing centrifuge tubes 4, blue application of sample centrifuge tube 5, DNA sample pipetting volume damping fluid 6,7, six DNA samples 8 of ethidium bromide, and test kit service manual 9.Wherein, TAE electrophoretic buffer 2 is 50 times of concentrated solution 60ml, and agarose 3 is 3.2g; Six look packing centrifuge tubes 4, every kind of color adopts the pipe of 10 1.5ml, is used for six DNA samples of packing; The blue application of sample centrifuge tube 5 of 10 1.5ml is used for packing DNA sample pipetting volume damping fluid 6, DNA sample pipetting volume damping fluid 6 is 6 times of concentrated solutions, 200 μ l, ethidium bromide 7 is for being adsorbed with spissated ethidium bromide solution and exsiccant disk filter paper, is stored in the white 5ml centrifuge tube and adopts masking foil parcel lucifuge; Six DNA sample 8 each 120 μ l comprise 5 suspect S with different restriction enzyme digestion and electrophoresis bands of a spectrum ₁, S ₂, S ₃, S ₄, S ₅The sample of sample and crime scene CS.

In the test kit in above-mentioned each small packages the consumption of material be the optimal value that experimental teaching over the years obtains, one group of material uses for one group of student (2-3 people), ten groups of materials can be carried out experiment for a standard class (30 people).

During experiment, each experiment group is assigned in every group of material taking-up in the test kit, according to the explanation of service manual, the teacher explains and can finish experiment by instruction of papil.In addition, can also dispose corresponding experiments supporting in the test kit according to different needs and instruct teaching material and CD, combining with teaching experimentizes, better effects if.

Claims (2)

1, the teaching kit that a kind of dna fingerprinting is analyzed, it is characterized in that comprising in the test kit (1) TAE electrophoretic buffer (2), agarose (3), six look packing centrifuge tubes (4), blue application of sample centrifuge tube (5), DNA sample pipetting volume damping fluid (6), ethidium bromide (7), six DNA samples (8) and test kit service manual (9), wherein, TAE electrophoretic buffer (2) is 50 times of concentrated solution 60ml, agarose (3) is 3.2g, and six look packing centrifuge tubes (4) are used for six DNA samples of packing, and every kind of color adopts the pipe of 10 1.5ml, the blue application of sample centrifuge tube (5) of 10 1.5ml is used for packing DNA sample pipetting volume damping fluid (6), and DNA sample pipetting volume damping fluid (6) is 6 times of concentrated solutions, 200 μ l; Described ethidium bromide (7) is for being adsorbed with spissated ethidium bromide solution and exsiccant disk filter paper, is stored in the white 5ml centrifuge tube and adopts masking foil parcel lucifuge; Described six DNA samples (8) respectively are 120 μ l, comprise 5 suspects' with different restriction enzyme digestion and electrophoresis bands of a spectrum the sample and the sample of a crime scene.

2, the teaching kit of analyzing according to the dna fingerprinting of claim 1 is characterized in that also being equipped with experiment instruction teaching material and CD in the test kit (1).

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