CN100410651C - Teaching kit for water contamination indication of horsebean micronucleus - Google Patents
Teaching kit for water contamination indication of horsebean micronucleus Download PDFInfo
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- CN100410651C CN100410651C CNB2006100232447A CN200610023244A CN100410651C CN 100410651 C CN100410651 C CN 100410651C CN B2006100232447 A CNB2006100232447 A CN B2006100232447A CN 200610023244 A CN200610023244 A CN 200610023244A CN 100410651 C CN100410651 C CN 100410651C
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Abstract
The present invention relates to a teaching kit for the indication of horsebean micronucleus to environmental pollution. Aiming at the application experiment of the horsebean micronucleus experiment in environment monitoring, reagents and material can be respectively filled in the kit in a packet mode by dividing groups, and the optimizing configuration can be carried out according to ten groups with one experiment teaching standard class. Each group of packets comprises glass slide, water absorption filter paper, cover glass, a 5 ml four-color centrifuge tube, an improved phenol fuchsine staining solution, etc. In addition, the packets are also provided with fixing liquid needing to be prepared and divided by experiment teachers for packing before an experiment, hydrolyzing separating liquid and distilled water processing horsebean root tips, cyclophosphamide processing horsebean root tips and a service manual. The present invention can be applied to the introduction teaching of a bioscience experiment, and can save the experiment preparation time for teachers and reduce the configuration of experimenters and the technical difficulty of experiment preparation. The application experiment of the horsebean micronucleus experiment in environment monitoring can be quickly completed in high efficiency, and favorable experiment effect can be obtained.
Description
Technical field
The present invention relates to a kind of teaching kit of indication of broad bean micronucleus environmental pollution, be used for the application experiment of biological scientific experiment introduction teaching broad bean micronucleus test in environmental monitoring.
Background technology
At present, China's biological scientific experiment teaching faces to have high input, produce imitates low awkward situation, and general laboratory all needs to dispose simultaneously experiment teacher and laboratory technician.Before experiment, need to do a large amount of preliminary work, often will buy earlier and test relevant plurality of reagents and material, the preparation of dividing into groups of the number situation of experimental then requirement, experiment.Experimenter's configuration that the finishing of these work needs some, reagent of Gou Maiing and material be often all greater than the consumption of actual needs simultaneously, thereby existence waste in various degree, improved experimental cost.The technical difficulty of testing preliminary work simultaneously is also very high, and to the use amount of each experiment group, to the accurate tolerance and the distribution of every kind of reagent, every kind of material usage, it is careful accurate all to require, to guarantee normally carrying out of experiment.This shows that preparing experiment need consume and waste great amount of manpower and material resources, financial resources, be badly in need of a kind of mode of new preparing experiment.Though it is very general that domestic and imported scientific research kit is used in China's bio-science research, have convenient, fast, efficient and result's characteristics accurately and reliably, but do not see the kit patented product that is applicable to the teaching of biological scientific experiment introduction at home so far as yet.Because the scientific research kit generally all at a certain particular experiment link, has very strong technical characterictic, its content is difficult to the education experiment on the adaptive surface simultaneously.Thereby, invent a kind of teaching kit of biological scientific experiment teaching that is applicable to and seem particularly necessary and urgent.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art and actual needs, a kind of teaching kit that is applicable to the indication of broad bean micronucleus environmental pollution is provided, can directly be used for finishing education experiment easily, guarantee the high-level efficiency of experiment, save experimental cost.
For realizing such purpose, the present invention is directed to the application experiment of broad bean micronucleus test in environmental monitoring, a part of reagent and material are assembled in the kit with inner wrapping form branch respectively, and be optimized configuration by 10 groups one experimental teaching standard class, material in every group in each inner wrapping is according to the use amount design of an experiment group, every group of inner wrapping comprises microslide, absorbent filter, cover glass, four look 5ml centrifuge tubes, improve the pinkish red dye liquor of phenol etc., also having needs the experiment teacher to test the immobile liquid of preceding matching while using and packing, the hydrolysis parting liquid and the broad bean tip of a root.Ten groups of inner wrappings are housed in each kit, and are equipped with service manual, can carry out experiment for a standard class.
Teaching kit of the present invention contains a complete set of material and the reagent of the application experiment of broad bean micronucleus test in environmental monitoring.10 groups of inner wrapping materials are laid in grouping in the kit, and every group comprises one bag of microslide, a bag absorbent filter, and a pkt. flap slide, 5ml centrifuge tube (four kinds of colors), one bottle of pinkish red dye liquor of improvement phenol.The consumption of every group of inner wrapping reagent and material is that wherein microslide is 4 every bag, 5 every bag of cover glasses, every bottle of 4ml of the pinkish red dye liquor of improvement phenol, about 10 of the every bag of absorbent filter through the experimental teaching optimal value groping to obtain repeatedly over the years.In the described four look 5ml centrifuge tubes, each one of dichromatism 5ml centrifuge tube is used for packing distilled water and handles the broad bean tip of a root and the endoxan processing broad bean tip of a root; Dichromatism 5ml centrifuge tube is each two in addition, is used for packing immobile liquid and hydrolysis parting liquid.Be equipped with immobile liquid component glacial acetic acid 20ml, immobile liquid component absolute ethyl alcohol 60ml and hydrolysis parting liquid 80ml in the kit in addition, distilled water handles the broad bean tip of a root and endoxan is handled the broad bean tip of a root, and the kit service manual, wherein handle every kind each 10 at the broad bean tip of a root for two kinds.
One group of material can use for one group of student (2-3 people) in the kit of the present invention, and ten groups of materials can be carried out experiment for a standard class (30 people).Can also be equipped with corresponding experiment instruction teaching material and CD according to different needs.
Half an hour before the experiment,, experiment teacher or preparation personnel need prepare immobile liquid and experiment material packing.Immobile liquid component glacial acetic acid is poured in the immobile liquid component absolute ethyl alcohol bottle, and mixing is prepared into fresh immobile liquid; Distilled water is handled root and endoxan handle the root branch and install in the dichromatism centrifuge tube, each adds the 4ml immobile liquid, is distributed to each experimental group; Immobile liquid and hydrolysis parting liquid branch are installed in other the dichromatism centrifuge tube, two of every kind of homochromy centrifuge tubes of reagent packing, every pipe 4ml is distributed to each experimental group.During experiment, only need allow the student take out of microscope, can begin experiment: distilled water will be housed handle the immobile liquid that the broad bean tip of a root and endoxan handle in the centrifuge tube of the broad bean tip of a root, and add the hydrolysis parting liquid; After about 15 minutes, anhydrate and separate parting liquid, add immobile liquid; Remove immobile liquid after 5 minutes, with the weep tap water flushing tip of a root; Cut tip of a root separate living tissue on microslide, be cut into several sections in length and breadth, cross method compressing tablet fully disperses cell then, and separately two slides dye.During dyeing, the pinkish red dye liquor of improvement phenol need cover all cells, with abundant dyeing.After dying lechery, add cover glass, inhale with thieving paper and remove unnecessary dye liquor.Note in the experiment, can obtain two compressing tablets behind the sharp one by one compressing tablet, so every group of experiment needs 4 of microslides.The consumption of reagent and material is distributed rationally in the kit, can satisfy the experiment needs, guarantees the carrying out of experiment, can accomplish on the other hand to save.
Teaching kit of the present invention provides distilled water to handle the broad bean tip of a root and endoxan is handled the broad bean tip of a root, save the Seed Treatment step of complicated and time consumption, the micronuclear rates that has guaranteed the tip of a root again accurately and reliably, can make the experiment teacher only need very simple experiment to prepare just can finish the student experimenting teaching, this has reduced experimenter's configuration on the one hand, also make experimental teaching quick, efficient on the other hand, the student can obtain standard of comparison, reliable experimental result.
The present invention provides a kind of economical and practical teaching kit for the teaching of biological scientific experiment introduction, experiment reagent and material are adopted inner wrapping and be optimized configuration by 10 groups one experimental teaching standard class, avoid unnecessary waste, saved reagent and material.During experiment, after the teacher reads the kit service manual, need only according to explanation every group of required material and reagent are distributed to each group in conjunction with explaining the teaching that can experimentize, saved loaded down with trivial details experiment and prepared the time and efforts of having saved the teacher.Simultaneously, the raising of work efficiency makes experimenter's configuration that certain minimizing can be arranged.The service manual that the student has according to teacher's classroom explanation and kit can have good grasp and finish experiment smoothly experiment content in the short period of time.Kit of the present invention makes the application experiment of broad bean micronucleus test in environmental monitoring can finish and obtain good experiment effect efficiently, fast, and financial resources, material resources, the manpower that experiment can be prepared required consumption drop to minimum.
Description of drawings
Fig. 1 is the reagent that kit of the present invention comprised and the displaying of medicine.
Among the figure, 1. kit, 2. immobile liquid component glacial acetic acid 20ml, 3. immobile liquid component absolute ethyl alcohol 60ml, 4. hydrolysis parting liquid, 5. distilled water is handled the broad bean tip of a root, and 6. endoxan is handled the broad bean tip of a root, 7. microslide, 8. absorbent filter, 9. cover glass, 10. four look 5ml centrifuge tubes are 6,11. the pinkish red dye liquor of improvement phenol, 12. kit service manuals
Embodiment
Below in conjunction with accompanying drawing technical scheme of the present invention is further described.
Fig. 1 is an embodiment of teaching kit provided by the invention, has comprised required a complete set of material and the reagent of the application experiment of broad bean micronucleus test in environmental monitoring in the kit.As shown in Figure 1, part experiment reagent and material adopt inner wrapping and are optimized configuration by 10 groups one experimental teaching standard class, 10 groups of inner wrapping materials are laid in grouping in the kit 1, material in every group is the use amount of an experiment group, every group comprises one bag of 4 microslide 7,10 absorbent filters 8 of one bag, one bag of 5 cover glass 9, four look 5ml centrifuge tubes 10, one bottle of pinkish red dye liquor 11 of 4ml improvement phenol, in the wherein said four look 5ml centrifuge tubes (10), the dichromatism centrifuge tube each one, in addition the dichromatism centrifuge tube is each two; Kit is equipped with 20ml immobile liquid component glacial acetic acid 2 in addition, 60ml immobile liquid component absolute ethyl alcohol 3, and 4,10 distilled water of 80ml hydrolysis parting liquid are handled 5,10 endoxan of the broad bean tip of a root and are handled the broad bean tip of a root 6 and a kit service manual 12.
In the kit in above-mentioned each inner wrapping the consumption of material be the optimal value that experimental teaching over the years obtains, one group of material uses for one group of student (2-3 people), ten groups of materials can be carried out experiment for a standard class (30 people).
During experiment, each experiment group is assigned in every group of material taking-up in the kit, according to the explanation of service manual, the teacher explains and can finish experiment by instruction of papil.In addition, can also dispose corresponding experiments supporting in the kit according to different needs and instruct teaching material and CD, combining with teaching experimentizes, better effects if.
Claims (2)
1. the teaching kit of the indication of a broad bean micronucleus environmental pollution, it is characterized in that part reagent that the application experiment of broad bean micronucleus test in environmental monitoring is required and material are assembled in the kit with inner wrapping form branch respectively, and be optimized configuration by 10 groups one experimental teaching standard class; Material in every group comprises one bag 4 microslides (7), one bag 10 absorbent filters (8), one bag 5 cover glasses (9), four look 5ml centrifuge tubes (10), one bottle of pinkish red dye liquor (11) of 4ml improvement phenol, in the wherein said four look 5ml centrifuge tubes (10), each one of dichromatism centrifuge tube, the dichromatism centrifuge tube is each two in addition; Kit is equipped with 20ml immobile liquid component glacial acetic acid (2) in addition, 60ml immobile liquid component absolute ethyl alcohol (3), 80ml hydrolysis parting liquid (4), 10 distilled water are handled the broad bean tip of a root (5), and 10 endoxan are handled the broad bean tip of a root (6) and a kit service manual (12).
2. according to the teaching kit of the indication of the broad bean micronucleus environmental pollution of claim 1, it is characterized in that also being equipped with experiment instruction teaching material and CD in the kit (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100232447A CN100410651C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for water contamination indication of horsebean micronucleus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100232447A CN100410651C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for water contamination indication of horsebean micronucleus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1804598A CN1804598A (en) | 2006-07-19 |
| CN100410651C true CN100410651C (en) | 2008-08-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100232447A Expired - Fee Related CN100410651C (en) | 2006-01-12 | 2006-01-12 | Teaching kit for water contamination indication of horsebean micronucleus |
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| Country | Link |
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| CN (1) | CN100410651C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101131385B (en) * | 2007-09-17 | 2010-11-10 | 淮海工学院 | Rotifer Brachionus plicatilis microkernel preparation technology |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624638A (en) * | 1993-05-05 | 1997-04-29 | Davcotech, Inc. | Modular laboratory equipment and coupling system |
| US20030207456A1 (en) * | 1998-09-18 | 2003-11-06 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| CN1181330C (en) * | 1999-04-23 | 2004-12-22 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic dyeing equipment for dyeing micro sample |
-
2006
- 2006-01-12 CN CNB2006100232447A patent/CN100410651C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5624638A (en) * | 1993-05-05 | 1997-04-29 | Davcotech, Inc. | Modular laboratory equipment and coupling system |
| US20030207456A1 (en) * | 1998-09-18 | 2003-11-06 | Cytyc Corporation | Method and apparatus for preparing cytological specimens |
| CN1181330C (en) * | 1999-04-23 | 2004-12-22 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic dyeing equipment for dyeing micro sample |
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| Publication number | Publication date |
|---|---|
| CN1804598A (en) | 2006-07-19 |
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Granted publication date: 20080813 Termination date: 20110112 |