CN201586570U

CN201586570U - Plastic centrifugal filtration tube

Info

Publication number: CN201586570U
Application number: CN2010201276406U
Authority: CN
Inventors: 席杰军; 王锁民
Original assignee: Lanzhou University
Current assignee: Lanzhou University
Priority date: 2010-02-03
Filing date: 2010-02-03
Publication date: 2010-09-22
Anticipated expiration: 2020-02-03

Abstract

The utility model involves a centrifugal filtration tube used in analytical chemistry and molecular biology. A plastic centrifugal filtration tube includes a screw tube cover(1) provided on the centrifuge tube body (2), a centrifuge tube internal sleeve (3) provided in the centrifuge tube body (2), and a boss arranged on the upper end of the centrifuge tube internal sleeve (3) and supported on the centrifuge tube body (2), which is characterized in that a netted framework (3-1) is provided at the bottom of the centrifuge tube internal sleeve (3), a filter sheet (5) provided on the netted framework (3-1), and a filter sheet turgor pressure ring (6) compacts the filter sheet (5). The utility model has the advantage that only one time of low speed (2000-5000rpm) temporary (2-3 minutes) centrifugation is required, the utility model has simple, fast, efficient and low-cost merits, and can save a lot of manpower and time especially for scientific research work with large quantities of samples.

Description

Plastics centrifugal filtration pipe

Technical field:

The utility model relates to the centrifugal filtration pipe of using in a kind of analytical chemistry and the molecular biology.

Background technology:

In the modern analysis chemical field, high performance liquid chromatograph, liquid chromatography mass combined instrument and flow injection analyzer etc. are widely used in the detection and the research of environment and food.According to research purpose, after taking various technical research schemes that liquefaction such as sample such as water body, soil, meat, milk product, animal vegetable tissue are handled, sample after must handling liquefaction carries out " cleaning " to be handled, and just can analyze in above-mentioned large-scale high-precision instrument; Otherwise the last all product that contain a large amount of microparticles can stop up sample intake passage or its accurate pumping system of accelerated wear test of these large-scale precision instruments, and then these expensive large-scale instruments are often broken down or the life-span die-offs.And existing technical solution to this problem is: after liquefaction being handled back sample high speed centrifugation (8000-12000rpm) supernatant is taken out, manually just can go up sample with syringe and micron-sized filtering head after to the supernatant filtration again.Because the molecule that contains a large amount of different-diameters in the sample of back is handled in liquefaction, so high speed centrifugation makes its clarification to a certain degree need 10-20 minute, then also needs manually to refilter.Its shortcoming is: for the mass disposal sample number, and inefficiency, the consumption people is time-consuming.

In addition, with the plasmid of SDS alkaline lysis extracting heavy dose from bacterium, be experiment common in the modern molecular biology research work.Its roughly process be: after the sex change of bacterium alkaline lysis, bacterioprotein, cell membrane and the bacterial genomes DNA etc. of sex change are thoroughly removed from alkaline lysis solution, from " cleaning " supernatant, reclaim DNA then; Otherwise, the DNA of extracting can contain foreign protein and bacterial genomes, this will have a strong impact on the progress of downstream experiment, as cause the decline of expensive DNA endonuclease activity thereby need a large amount of restriction endonuclease preparations or because the failure that the genomic DNA pollution directly causes the downstream to test.And existing technical solution to this problem is: by to alkaline lysis solution repeatedly high speed centrifugation to make supernatant " cleaning " as far as possible.Its shortcoming is: since contain in the alkaline lysis solution a large amount of different-diameters microparticle and centrifugal repeatedly also be difficult to remove to the greatest extent and molecular biology experiment itself to polluting and responsive, so usually cause downstream the failure of an experiment.In the scientific research field of modern forward position, each scientific research project all comprises numerous experimental procedures, and finishing of each experimental procedure all needs to expend plenty of time and painstaking effort; Therefore, to cause whole scientific research project period planning to baffle or make whole previous experiments all that has been achieved is spoiled be unacceptable because of some annelet goes wrong.

The content of utility model:

The purpose of this utility model is to avoid the deficiencies in the prior art part and a kind of plastics centrifugal filtration pipe is provided, and it is efficient more, quick, convenient that feasible solution to the problems referred to above becomes.

The purpose of this utility model can be by realizing by the following technical solutions: a kind of plastics centrifugal filtration pipe, include and on centrifugal body (2), be provided with screw socket pipe lid (1), in centrifugal body (2), be provided with cover (3) in the centrifuge tube, being provided with boss on the cover (3) in the centrifuge tube is supported on the centrifugal body (2), its main feature is also to include and is provided with mesh skeleton (3-1) in the centrifuge tube bottom the cover (3), mesh skeleton (3-1) is provided with filter disc (5), and filter disc turgescence circle (6) compresses filter disc (5).

Described plastics centrifugal filtration pipe, also including in the described centrifuge tube is hollow bottom the cover (3), and its bottom margin is the boss (3-2) that has groove, and the boss at skeleton (4) edge is decided in riveting and the groove of centrifuge tube interior cover (3) boss (3-2) is combined closely; Decide to be provided with mesh skeleton (4-1) on the skeleton (4) in riveting, mesh skeleton (4-1) is provided with filter disc (5), and filter disc turgescence circle (6) compresses filter disc (5); Riveting network framework turgescence circle (7) compresses riveting and decides skeleton (4).

The beneficial effects of the utility model: only need low speed (2000-5000rpm), the once centrifugal of of short duration (2-3 minute) to get final product, has easy, quick, advantage efficiently and cheaply, especially for the research work of batch samples number, can save great amount of manpower and time.

Description of drawings:

Fig. 1 is the overall structure schematic diagram of embodiment 1 of the present utility model.

Fig. 2 is the enlarged diagram of Figure 1A.

Fig. 3 is the overall structure schematic diagram of embodiment 2 of the present utility model.

Fig. 4 is the enlarged diagram of Fig. 3 B.

The specific embodiment:

Be described in further detail below in conjunction with the most preferred embodiment shown in the accompanying drawing:

Embodiment 1: see Fig. 1, Fig. 2, a kind of plastics centrifugal filtration pipe, include and on centrifugal body 2, be provided with screw socket pipe lid 1, be provided with in centrifugal body 2 and overlap 3 in the centrifuge tube, the interior cover of centrifuge tube 3 upper ends are provided with boss and are supported on the centrifugal body 2, and cover 3 bottoms are provided with mesh skeleton 3-1 in the centrifuge tube, mesh skeleton 3-1 is provided with filter disc 5, and filter disc turgescence circle 6 compresses filter disc 5.

Embodiment 2: see Fig. 3, Fig. 4, a kind of plastics centrifugal filtration pipe, include and on centrifugal body 2, be provided with screw socket pipe lid 1, be provided with in centrifugal body 2 and overlap 3 in the centrifuge tube, the interior cover of centrifuge tube 3 upper ends are provided with boss and are supported on the centrifugal body 2, and cover 3 bottoms are hollow in the described centrifuge tube, its bottom margin is the boss 3-2 that has groove, and the boss at skeleton 4 edges is decided in riveting and the groove of the interior cover of centrifuge tube 3 boss 3-2 is combined closely; Decide to be provided with mesh skeleton 4-1 on the skeleton 4 in riveting, mesh skeleton 4-1 is provided with filter disc 5, and filter disc turgescence circle 6 compresses filter disc 5; Riveting network framework turgescence circle 7 compresses riveting and decides skeleton 4.

Two kinds of wherein above-mentioned plastics centrifugal filtration pipes, in its centrifuge tube the height of cover 3 be centrifugal body 2 half.About the material of filter disc, can be the filter paper of different model, as quantitative filter paper or qualitative filter paper, the number of plies of its filter paper can be at the 3-8 layer; Also can be the acetate fiber filter paper of 0.2 μ m, 0.45 μ m, 0.5 μ m; It also can be glass fiber filter paper etc.The material of other parts is the plastics that can reach experiment purpose intensity.

Operation principle of the present utility model and using method are as follows:

To treat that " cleaning " fluid sample adds cover in the centrifuge tube, and tighten and after the screw socket pipe covers whole centrifugal filtration pipe is put into the of short duration low-speed centrifugal of centrifuge, and make and treat " cleaning " fluid sample, and then make the microparticle of a large amount of different-diameters obtain filtering by filter disc; " cleaning " filtered fluid of centrifuge tube bottom can directly carry out downstream process, as directly entering flow injection analyzer or direct plasmid DNA purification from filtered fluid.This process only needs of short duration (2-3 minute) once centrifugal getting final product of low speed (2000-5000rpm), has easy, quick, advantage efficiently and cheaply, especially for the research work of batch samples number, can save great amount of manpower and time.

The above only is preferred embodiment of the present utility model, and is in order to restriction the utility model, not all within spirit of the present utility model and principle, any modification of being done, is equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.

Claims

1. plastics centrifugal filtration pipe, include and on centrifugal body (2), be provided with screw socket pipe lid (1), in centrifugal body (2), be provided with cover (3) in the centrifuge tube, being provided with boss on the cover (3) in the centrifuge tube is supported on the centrifugal body (2), it is characterized in that also including and be provided with mesh skeleton (3-1) in the centrifuge tube bottom the cover (3), mesh skeleton (3-1) is provided with filter disc (5), and filter disc turgescence circle (6) compresses filter disc (5).

2. plastics centrifugal filtration pipe as claimed in claim 1, it is characterized in that also including and be hollow in the described centrifuge tube bottom the cover (3), its bottom margin is the boss (3-2) that has groove, and the boss at skeleton (4) edge is decided in riveting and the groove of centrifuge tube interior cover (3) boss (3-2) is combined closely; Decide to be provided with mesh skeleton (4-1) on the skeleton (4) in riveting, mesh skeleton (4-1) is provided with filter disc (5), and filter disc turgescence circle (6) compresses filter disc (5); Riveting network framework turgescence circle (7) compresses riveting and decides skeleton (4).