CN216039533U - Novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument - Google Patents
Novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument Download PDFInfo
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- CN216039533U CN216039533U CN202122664455.1U CN202122664455U CN216039533U CN 216039533 U CN216039533 U CN 216039533U CN 202122664455 U CN202122664455 U CN 202122664455U CN 216039533 U CN216039533 U CN 216039533U
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Abstract
The utility model discloses a novel 96-pore plate magnetic labeling biological component enrichment, separation and extraction instrument which comprises a supporting plate, an equipment base, a first limiting block and a culture plate, wherein the top end of the supporting plate is provided with the culture plate, the bottom end of the culture plate is fixedly connected with the supporting plate, the top end of the supporting plate at one side of the culture plate is provided with a movable chamber cover, the top end of the movable chamber cover is provided with ninety-six culture plate single holes at equal intervals, a magnetic head handle is arranged inside each single hole of the culture plate, and the bottom end of the magnetic head handle extends to the inside of the culture plate. The utility model not only realizes the enrichment separation of the novel 96-pore plate magnetic labeling biological components and the uniform and complete adsorption of the magnetic particles by the extraction instrument, facilitates the direct cell staining identification of the magnetic particles in the culture plate, but also improves the cell staining identification efficiency.
Description
Technical Field
The utility model relates to the technical field of cell separation and staining equipment, in particular to a novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument.
Background
With the development of science, the research on chromosome is an indispensable link in the biological industry, the rapid acquisition of a large number of ginseng cell chromosomes with complete structures, no cytoplasm and nucleus and single purer structure is the basis of a plurality of research works, such as the electron microscope structural analysis of chromosome, the biochemical analysis and the preliminary purification of chromosome structural protein, the preparation of a whole chromosome probe pool, gene localization, gene cloning, library construction, the realization of germplasm resource innovation by utilizing specific chromosome for distant hybridization and the like, the traditional cell sorting is mostly carried out under the macro scale, such as centrifugal sorting, chemical dissolution, antibody labeling sorting based on fluorescence or magnetic bead technology, etc., which are mature and reliable, but have complex system, complicated operation, large sample amount, the target cells are easily lost in the links of cell transfer, container replacement, sample chemical treatment and the like.
The cell separation and staining equipment on the market is various and can basically meet the use requirements of people, but certain problems still exist, when the existing magnetic head adsorption device absorbs magnetic particles in a six-hole culture plate, usually, small rod-shaped magnets or electromagnets are used for continuously performing covering scanning in single holes of the six-hole culture plate, the scanning area of the magnetic rods or the electromagnets is difficult to uniformly cover the whole single hole of the six-hole culture plate, the scanning time is relatively long, staining solution is wasted more, and the staining time is long.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a novel 96-well plate magnetic labeling biological component enrichment separation and extraction instrument, which aims to solve the problems that cell separation and staining equipment is inconvenient to uniformly and completely adsorb magnetic particles and cell staining identification is directly carried out in a culture plate in the background technology.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a novel 96 orifice plate magnetic marking biological component enrichment separation and extraction apparatus, includes backup pad, equipment base, first stopper and cultivation board, the cultivation board is installed on the top of backup pad, and the bottom and the backup pad fixed connection of cultivation board, the backup pad top of cultivation board one side is equipped with movable chamber cover, ninety six holes of equidistant cultivation board haplopore are installed on the top of activity chamber cover, the inside of cultivation board haplopore is equipped with the magnetic head handle, and the bottom of magnetic head handle extends to the inside of cultivation board, the inside of cultivation board is equipped with equidistant twelve collecting tanks, the inside twelve sink that is equipped with equidistant of cultivation board of collecting tank one side, the inside twelve fixed solution tanks that are equipped with equidistant of cultivation board of sink one side.
Preferably, twelve deep color liquid tanks are arranged at equal intervals in the culture plate on one side of the fixed solution tank, and twelve first washing tanks are arranged at equal intervals in the culture plate on one side of the deep color liquid tanks.
Preferably, twelve DAPI staining solution tanks are arranged in the culture plate on one side of the first cleaning tank at equal intervals, twelve second cleaning tanks are arranged in the culture plate on one side of the DAPI staining solution tanks at equal intervals, and twelve PBS solution tanks are arranged in the culture plate on one side of the second cleaning tanks at equal intervals.
Preferably, a first limiting block is installed at the top end of the supporting plate on one side of the culture plate, and the bottom end of the first limiting block is fixedly connected with the supporting plate.
Preferably, a second limiting block is installed at one end, far away from the first limiting block, of the top of the supporting plate, and the bottom end of the second limiting block is fixedly connected with the supporting plate.
Preferably, the outer wall of the culture plate is provided with a second sealing ring, and the outer wall of the culture plate below the second sealing ring is provided with a first sealing ring.
Preferably, four groups of bolt sleeves are arranged at the bottom end of the equipment base, and the outer walls of the bolt sleeves are fixedly connected with the equipment base.
Preferably, the bolt sleeve is internally provided with an elongated bolt, and the top end of the elongated bolt extends to the inside of the support plate.
Compared with the prior art, the utility model has the beneficial effects that: the cell separation and staining device not only realizes the enrichment and separation of the novel 96-pore plate magnetic labeling biological components and the uniform and complete adsorption of the magnetic particles by the extraction instrument, facilitates the direct cell staining identification of the magnetic particles in the culture plate, but also improves the cell staining identification efficiency;
(1) when the magnetic particles are adsorbed by the magnetic head handle, the round permanent magnet is rotated for one circle or N circles, so that the magnetic particles in the culture plate can be uniformly and completely adsorbed, and cell staining identification is directly carried out in the culture plate, so that the novel 96-pore plate magnetic labeling biological component enrichment separation and extraction apparatus uniform and complete adsorption of the magnetic particles are realized, the magnetic particles can be conveniently and directly subjected to cell staining identification in the culture plate, and the cell staining identification efficiency is improved;
(2) the first limiting block and the supporting plate are fixed together, the second limiting block and the supporting plate are fixed together, and the culture plate is limited and supported under the cooperation of the first limiting block and the second limiting block, so that the limiting support of the cell separation and dyeing equipment with a convenient structure is realized, and the assembling speed of the cell separation and dyeing equipment is increased;
(3) when the movable chamber cover is installed together with the supporting plate, the culture plate is supported and connected with the supporting plate through the first sealing ring and the second sealing ring, the lengthened bolt is inserted into the bolt sleeve, the equipment base is fixed with the supporting plate through the lengthened bolt, the cell separation and dyeing equipment is convenient to install and fix, and the cell separation and dyeing equipment is convenient to disassemble and maintain at the later stage.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional explosive structure according to the present invention;
FIG. 2 is a schematic bottom view of the present invention;
FIG. 3 is a schematic three-dimensional structure of the movable chamber cover of the present invention;
FIG. 4 is a schematic three-dimensional structure of a plate according to the present invention;
fig. 5 is a schematic three-dimensional structure diagram of the apparatus base of the present invention.
In the figure: 1. a support plate; 2. an equipment base; 3. a first stopper; 4. a first seal ring; 5. a second seal ring; 6. culturing the plate; 7. a movable chamber cover; 8. single well of culture plate; 9. a magnetic head handle; 10. a second limiting block; 11. a bolt sleeve; 12. lengthening the bolt; 13. collecting tank; 14. a washing tank; 15. a fixed solution tank; 16. a dark liquid tank; 17. a first cleaning tank; 18. a DAPI staining fluid bath; 19. a second cleaning tank; 20. PBS solution tank.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, an embodiment of the present invention is shown: a novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument comprises a supporting plate 1, an equipment base 2, a first limiting block 3 and a culture plate 6, wherein the culture plate 6 is installed at the top end of the supporting plate 1, the bottom end of the culture plate 6 is fixedly connected with the supporting plate 1, a movable chamber cover 7 is arranged at the top end of the supporting plate 1 on one side of the culture plate 6, ninety-six culture plate single holes 8 with equal intervals are installed at the top end of the movable chamber cover 7, a magnetic head handle 9 is arranged inside each culture plate single hole 8, the bottom end of the magnetic head handle 9 extends into the culture plate 6, twelve collecting tanks 13 with equal intervals are arranged inside the culture plate 6 on one side of each collecting tank 13, twelve washing tanks 14 with equal intervals are arranged inside the culture plate 6 on one side of each washing tank 14, twelve deep color liquid tanks 16 with equal intervals are arranged inside the culture plate 6 on one side of each fixing solution tank 15, twelve equally-spaced first cleaning tanks 17 are arranged in the culture plate 6 on one side of the deep color liquid tank 16, twelve equally-spaced DAPI staining liquid tanks 18 are arranged in the culture plate 6 on one side of the first cleaning tanks 17, twelve equally-spaced second cleaning tanks 19 are arranged in the culture plate 6 on one side of the DAPI staining liquid tanks 18, and twelve equally-spaced PBS solution tanks 20 are arranged in the culture plate 6 on one side of the second cleaning tanks 19;
when the culture plate is used, the movable chamber cover 7 is sleeved with the support plate 1, the culture plate 6 is arranged on the surface of the support plate 1, the magnetic head handle 9 adopts a circular permanent magnet, the size of the magnetic head handle 9 is slightly smaller than the single hole 8 of the culture plate, when the magnetic head handle 9 adsorbs magnetic particles, the circular permanent magnet only needs to ensure that the distance height between the bottom surface of the magnet and the bottom of the culture plate 6 is consistent all the time, the center of the circular permanent magnet is aligned with the single hole 8 of the culture plate, the magnetic particles in the culture plate 6 can be uniformly and completely adsorbed by rotating the circular permanent magnet for one circle or N circles, cell staining identification is directly carried out in the culture plate, when the magnetic head handle 9 is used, the magnetic head is rotated to adsorb the magnetic particles in a sample collecting tank 13, cells wrapped by the magnetic particles are adhered to the magnetic head cap, and the magnetic head with the magnetic particles is horizontally moved to a second row of washing tanks 14 by the magnetic head handle 9, cleaning the magnetic head cap by moving the magnetic head handle 9 up and down, left and right to remove impurities and some magnetic particles, translating the magnetic head cleaned with the magnetic particles into a third row of fixed solution tanks 15 by the magnetic head handle 9, directly soaking the magnetic head cap with the magnetic particle-wrapped cells in the solution, translating the magnetic head into a fourth row of dark color liquid tanks 16 by the magnetic head handle 9, soaking the magnetic head cap into the dark color liquid, dyeing the cells in red and blue, wherein the dyeing time needs thirty minutes, translating the magnetic head into a fifth row of first cleaning tanks 17 of the culture plate by the magnetic head handle 9 after thirty minutes to clean residual dyeing liquid, translating the magnetic head into a sixth row of DAPI dyeing liquid tanks 18 of the culture plate by the magnetic head handle 9, wherein the dyeing time needs five minutes, the dyeing is completed after five minutes, translating the magnetic head into a seventh row of second cleaning tanks 19 of the culture plate by the magnetic head handle 9, the magnetic head is moved to the eighth row of PBS solution tank 20 of the culture plate through the magnetic head handle 9, the cells wrapped by the dyed magnetic particles are released in the PBS solution, so that the cell separation and dyeing work is finished, the dyed cells can be directly observed and analyzed under a microscope, the novel 96-hole plate magnetic labeling biological component enrichment separation and uniform and complete magnetic particle adsorption of an extraction instrument are realized, the magnetic particles can be conveniently and directly subjected to cell dyeing identification in the culture plate, and the cell dyeing identification efficiency is improved;
a first limiting block 3 is installed at the top end of the supporting plate 1 on one side of the culture plate 6, the first limiting block 3 plays a role of limiting and supporting, the bottom end of the first limiting block 3 is fixedly connected with the supporting plate 1, a second limiting block 10 is installed at one end, far away from the first limiting block 3, of the top of the supporting plate 1, the bottom end of the second limiting block 10 is fixedly connected with the supporting plate 1, and the second limiting block 10 plays a role of limiting and supporting;
when the cell separation and dyeing device is used, the first limiting block 3 is fixed with the supporting plate 1, the second limiting block 10 is fixed with the supporting plate 1, and the culture plate 6 is supported in a limiting manner under the matching of the first limiting block 3 and the second limiting block 10, so that the culture plate 6 and the supporting plate 1 can be conveniently installed and fixed, the limiting support of the cell separation and dyeing device with a convenient structure is realized, and the assembling speed of the cell separation and dyeing device is increased;
a second sealing ring 5 is arranged on the outer wall of the culture plate 6, a first sealing ring 4 is arranged on the outer wall of the culture plate 6 below the second sealing ring 5, four groups of bolt sleeves 11 are arranged at the bottom end of the equipment base 2, the outer walls of the bolt sleeves 11 are fixedly connected with the equipment base 2, lengthening bolts 12 are arranged inside the bolt sleeves 11, the top ends of the lengthening bolts 12 extend into the supporting plate 1, and the lengthening bolts 12 play a role in connection and fixation;
during the use through installing second sealing washer 5 on the surface of culture plate 6, install first sealing washer 4 on the surface of culture plate 6, when activity chamber cover 7 is installed together with backup pad 1, culture plate 6 supports with backup pad 1 through first sealing washer 4 and second sealing washer 5 and is connected, play the supporting role once more to culture plate 6, insert the inside of extension bolt 12 in bolt cover 11, equipment base 2 is fixed through extension bolt 12 and backup pad 1, make things convenient for backup pad 1 and equipment base 2 to break away from, it is fixed to have realized that cell separation dyeing is equipped convenient installation, the dismantlement maintenance in cell separation dyeing equipment later stage has been made things convenient for.
When the embodiment of the application is used: firstly, the movable chamber cover 7 is sleeved with the support plate 1, the culture plate 6 is arranged on the surface of the support plate 1, the magnetic head handle 9 adopts a circular permanent magnet, the size of the magnetic head handle 9 is slightly smaller than the single hole 8 of the culture plate, when the magnetic head handle 9 adsorbs magnetic particles, the circular permanent magnet only needs to ensure that the distance height between the bottom surface of the magnet and the bottom of the culture plate 6 is always consistent, the center of the circular permanent magnet is aligned with the single hole 8 of the culture plate, the magnetic particles in the culture plate 6 can be uniformly and completely adsorbed by rotating the circular permanent magnet for one circle or N circles, cell staining identification is directly carried out in the culture plate, when in use, the magnetic head is rotated by the magnetic head handle 9 to adsorb the magnetic particles in a sample 13, cells wrapped by the magnetic particles are adhered to the magnetic head cap, and the magnetic head with the magnetic particles is horizontally moved to the second row of washing tanks 14 by the magnetic head handle 9, cleaning the magnetic head cap by moving the magnetic head handle 9 up and down, left and right to remove impurities and some magnetic particles, translating the magnetic head cleaned with the magnetic particles into a third row of fixed solution tanks 15 by the magnetic head handle 9, directly soaking the magnetic head cap with the magnetic particle-wrapped cells in the solution, translating the magnetic head into a fourth row of dark color liquid tanks 16 by the magnetic head handle 9, soaking the magnetic head cap into the dark color liquid, dyeing the cells in red and blue, wherein the dyeing time needs thirty minutes, translating the magnetic head into a fifth row of first cleaning tanks 17 of the culture plate by the magnetic head handle 9 after thirty minutes to clean residual dyeing liquid, translating the magnetic head into a sixth row of DAPI dyeing liquid tanks 18 of the culture plate by the magnetic head handle 9, wherein the dyeing time needs five minutes, the dyeing is completed after five minutes, translating the magnetic head into a seventh row of second cleaning tanks 19 of the culture plate by the magnetic head handle 9, used for washing away residual staining solution, the magnetic head is translated to the eighth row PBS solution tank 20 of the culture plate through the magnetic head handle 9, the cells wrapped by the stained magnetic particles are released in the PBS solution, so that the cell separation and staining work is finished, the stained cells can be directly observed and analyzed under a microscope, then the culture plate 6 and the support plate 1 are conveniently installed and fixed through fixing the first limit block 3 and the support plate 1 and fixing the second limit block 10 and the support plate 1, the culture plate 6 is supported under the matching of the first limit block 3 and the second limit block 10, the first seal ring 4 is installed on the surface of the culture plate 6 through installing the second seal ring 5 on the surface of the culture plate 6, when the movable chamber cover 7 is installed with the support plate 1, the culture plate 6 is supported and connected with the support plate 1 through the first seal ring 4 and the second seal ring 5, play the supporting role again to culture plate 6, insert extension bolt 12 in the inside of bolt cover 11, equipment base 2 is fixed through extension bolt 12 and backup pad 1, makes things convenient for backup pad 1 and equipment base 2 to break away from, accomplishes the use work that cell separation dyeing was equipped.
Claims (8)
1. A novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument is characterized in that: comprises a supporting plate (1), an equipment base (2), a first limiting block (3) and a culture plate (6), wherein the culture plate (6) is arranged at the top end of the supporting plate (1), the bottom end of the culture plate (6) is fixedly connected with the supporting plate (1), a movable chamber cover (7) is arranged at the top end of the supporting plate (1) at one side of the culture plate (6), ninety-six culture plate single holes (8) with equal intervals are arranged at the top end of the movable chamber cover (7), a magnetic head handle (9) is arranged inside each culture plate single hole (8), the bottom end of the magnetic head handle (9) extends to the interior of the culture plate (6), twelve collecting grooves (13) with equal intervals are arranged in the interior of the culture plate (6), twelve washing tanks (14) which are arranged at equal intervals are arranged in the culture plate (6) at one side of the collecting tank (13), twelve fixed solution tanks (15) are arranged in the culture plate (6) at one side of the washing tank (14) at equal intervals.
2. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 1, characterized in that: twelve deep color liquid tanks (16) which are equally spaced are arranged in the culture plate (6) at one side of the fixed solution tank (15), and twelve first cleaning tanks (17) which are equally spaced are arranged in the culture plate (6) at one side of the deep color liquid tanks (16).
3. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 2, characterized in that: twelve DAPI staining liquid tanks (18) with equal intervals are arranged inside the culture plate (6) at one side of the first cleaning tank (17), twelve second cleaning tanks (19) with equal intervals are arranged inside the culture plate (6) at one side of the DAPI staining liquid tanks (18), and twelve PBS solution tanks (20) with equal intervals are arranged inside the culture plate (6) at one side of the second cleaning tanks (19).
4. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 1, characterized in that: first stopper (3) are installed on backup pad (1) top of cultivateing board (6) one side, and the bottom and backup pad (1) fixed connection of first stopper (3).
5. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 1, characterized in that: the second limiting block (10) is installed at one end, far away from the first limiting block (3), of the top of the supporting plate (1), and the bottom end of the second limiting block (10) is fixedly connected with the supporting plate (1).
6. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 1, characterized in that: the outer wall of the culture plate (6) is provided with a second sealing ring (5), and the outer wall of the culture plate (6) below the second sealing ring (5) is provided with a first sealing ring (4).
7. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 1, characterized in that: four groups of bolt sleeves (11) are arranged at the bottom end of the equipment base (2), and the outer walls of the bolt sleeves (11) are fixedly connected with the equipment base (2).
8. The novel 96-well plate magnetic labeling biological component enrichment, separation and extraction instrument of claim 7, characterized in that: the bolt sleeve (11) is internally provided with a lengthened bolt (12), and the top end of the lengthened bolt (12) extends to the inside of the support plate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122664455.1U CN216039533U (en) | 2021-11-02 | 2021-11-02 | Novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122664455.1U CN216039533U (en) | 2021-11-02 | 2021-11-02 | Novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument |
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| CN216039533U true CN216039533U (en) | 2022-03-15 |
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| CN202122664455.1U Active CN216039533U (en) | 2021-11-02 | 2021-11-02 | Novel 96-pore plate magnetic labeling biological component enrichment separation and extraction instrument |
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