CN109959540A - The microbody sample of deep-sea foraminifer quantitatively purifies and production method - Google Patents
The microbody sample of deep-sea foraminifer quantitatively purifies and production method Download PDFInfo
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- CN109959540A CN109959540A CN201711430803.0A CN201711430803A CN109959540A CN 109959540 A CN109959540 A CN 109959540A CN 201711430803 A CN201711430803 A CN 201711430803A CN 109959540 A CN109959540 A CN 109959540A
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- 241001147665 Foraminifera Species 0.000 title claims abstract description 34
- 210000002500 microbody Anatomy 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 239000004576 sand Substances 0.000 claims abstract description 26
- 238000000746 purification Methods 0.000 claims abstract description 10
- 239000013049 sediment Substances 0.000 claims abstract description 8
- 238000007873 sieving Methods 0.000 claims abstract description 7
- 238000005188 flotation Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 12
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 claims description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 241000282376 Panthera tigris Species 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 238000004043 dyeing Methods 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 2
- 238000005485 electric heating Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 238000007605 air drying Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 16
- 238000002474 experimental method Methods 0.000 abstract description 10
- 238000011160 research Methods 0.000 abstract description 7
- 238000004458 analytical method Methods 0.000 abstract description 5
- 238000011084 recovery Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 73
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 238000007598 dipping method Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/368—Mounting multiple samples in one block, e.g. TMA [Tissue Microarrays]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to biology community structures to compare analysis, different types of identification research, and specifically a kind of microbody sample of deep-sea foraminifer quantitatively purifies and production method.Acquisition halmeic deposit is dyed through fixed, then takes sediment sample to dry, is then impregnated and dispersed, sample is transferred in the bolting silk that aperture is 300 mesh, " sand hill " is made；It rinses " sand hill " and dries again, the sample in " sand hill " is subjected to flotation concentration, sieving, microsection can be obtained the microbody sample of deep-sea foraminifer after purification.The method of the present invention can be extracted effectively and purify the foraminifer sample in deposit, and Saving specimen is completely indeformable, and experimental implementation process is succinct, compare existing experimental method, recovery rate and purifying rate are high, sample integrality is good, program time shortens.
Description
Technical field
The present invention relates to biology community structures to compare analysis, different types of identification research, specifically a kind of deep-sea
The microbody sample of foraminifer quantitatively purifies and production method.
Background technique
Since halmeic deposit is in low temperature, high pressure and close to conditions such as carbonate compensation depths, so that absmal deposit
The most exquisite in texture of object, stickiness is high, and seepage of water is small, few containing the grains of sand, very hard when dry, very viscous after chance water, so tradition is extracted
Experiment lacks the quantitative purifying for being suitable for deep-sea sample and flaking method, and used stainless steel mesh is to deep-sea foraminifer shell wall
It destroys seriously, causes post analysis operational data that can not compare and wait technology barriers.In addition, commonsense method has halmeic deposit
The recovery rate of hole worm is low, impurity is more, quality of experiments is poor causes purification effect bad, and during experiment process cryogenic high pressure with
And carbonate oxidation etc., greatly reduce the accuracy of quality of research and result of study.
Summary of the invention
It is an object of that present invention to provide the methods that a kind of microbody sample of deep-sea foraminifer is quantitatively purified and made.
To achieve the above object, the technical solution adopted by the present invention are as follows:
A kind of microbody sample of deep-sea foraminifer quantitatively purifies and production method, and acquisition halmeic deposit is contaminated through fixed
Color then takes sediment sample to dry, and is then impregnated and is dispersed, and sample is transferred in the bolting silk that aperture is 300 mesh and is made
At " sand hill "；It rinses " sand hill " and dries again, the sample in " sand hill " is subjected to flotation concentration, sieving, microsection
Obtain the microbody sample of deep-sea foraminifer after purification.
The halmeic deposit of acquisition is fixed through alcohol, is dyed with the red solution of tiger, through electric heating after dyeing is fixed
Air dry oven is dried, and halmeic deposit is impregnated and dispersed with hydrogen peroxide after drying and processing, sample is transferred to
Aperture be 300 mesh bolting silk in " sand hill " is made, will " sand hill " rinse and dry again, then use carbon tetrachloride to sample into
Row flotation concentration, sieving, microsection can be obtained the microbody sample of deep-sea foraminifer after purification.
Sample wash and the experimentation of foraminifer drying carry out in the bolting silk that aperture is 300 mesh, then at filtering
Reason.
Sample is placed in the bolting silk that aperture is 300 mesh after the soaking dispersion, " sand hill " is made through bolting silk package, then
" sand hill " is rinsed together with sample with tap water.
The sieve using 0.15mm and 0.063mm carries out sieving separating processing.
Advantage for present invention:
1. Sample Purification on Single degree is high.Raw material halmeic deposit of the present invention has exquisite in texture, and stickiness is high, and seepage of water is small, contains
The features such as grains of sand are seldom, very hard when dry, very viscous after chance water.Tradition, which extracts experiment, to be directly placed into after drying deposit
It is rinsed in the sieve of 0.063mm, is not suitable for the extraction of deep-sea foraminifer sample.Steaming is added in the method for the present invention after the drying
Distilled water or tap water, sufficiently impregnating (1 day~2 days) can be such that sample is completely dispersed.The high sample of the content of organic matter is with concentration
3% hydrogen peroxide dipping sample 2 hours, can be such that sediment sample is completely dispersed.
2. sample rapid batch is handled.Traditional treatment method is to be scrubbed with writing brush to the deposit in mesh screen, usually
Technology can only handle a sample every time.The method of the present invention is directly to be rinsed with flowing water to " sand hill ", primary flushable number
A sample, more succinct rapidly, mass disposal is also time saving and energy saving, saves water resource.
3. sample structure integrity degree is high.Traditional extraction process is after sediment sample is put into sieve, with writing brush under flowing water
Filtration treatment is repeated, the processing time is long and writing brush can directly be contacted with sample, the foraminifer number of samples in halmeic deposit
Amount is few, and deep-sea erect-position benthonic foraminifera density is extremely low, sample quality crisp fritter, and traditional method for extracting rate is low, impurity is more, experiment
It is of poor quality to cause purification effect bad, and cryogenic high pressure and carbonate oxidation etc. during experiment process, cause sample damaged
Seriously, quality of research is greatly reduced.Deposit is put into the bolting silk of 300 mesh of aperture in the method for the present invention, and " sand hill " is made.
" sand hill " is soft, makes destruction of the very thin frangible foraminifer of shell wall from using stainless steel mesh collision in usual technology.
Teetertotter " sand hill " in clear water, is filtered by water flow exchange, can filter silt completely, while avoiding directly connecing
Sample is hit in touching, ensure that the integrality of sample.When experiment is also greatly reduced when being carried out with carbon tetrachloride later and suspended and handled
Between.Operating process is succinct, can effectively extract and purify the foraminifer sample in deposit, and deposit recovery rate is high, and sample is pure
Change degree is good, and sample keeps complete after purification, and the deep-sea foraminifer sample of acquisition keeps complete, provides by force for scientific research analysis and identification
Strong foundation.
4. Saving specimen utilization rate is high.It needs to be adhered to sample in fossil box with writing brush after sample extraction in traditional experiment and protect
It deposits, one, fossil box about stores foraminifer sample 300 or so.Foraminifer sample quantity in halmeic deposit is few, deep-sea
Erect-position benthonic foraminifera density is extremely low, if not only wasting deep-sea foraminifer sample, and save with traditional method for extracting and preservation
Effect is unable to reach requirement, wasting manpower and material resources and reasearch funds.The method of the present invention can picking sample 150 as needed
~300 or so are made sample slice, remaining sample bottling simultaneously it is labelled, can long-term preservation use, guarantee sample it is complete for a long time
It saves and is able to achieve utilization rate maximization.
Detailed description of the invention
Fig. 1 quantitatively purifies for the microbody sample of deep-sea foraminifer provided in an embodiment of the present invention and schematic diagram of manufacturing method.
Specific embodiment
Following embodiment is that the present invention is further described.
The present invention is quantitatively purified for the microbody sample of deep-sea foraminifer and production method.Pass through one to halmeic deposit
Serial experiment processing, key experimentation carry out in sand hill.Purification process can be to research deep-sea foraminifer Identification of Species, kind
Class composition, COMMUNITY STRUCTURE provide most effective experimental program, and the research and whole world change to deep-marine-environment instruction are ground
Study carefully and provide strong foundation, and the microcosmic microbody biological sample that can be saved for a long time can be provided for deep-sea work of popular science.
Embodiment 1
1, fixed dyeing
After collecting halmeic deposit sample, to sample carry out freezing processing or scene using alcohol (70% concentration and with
On) be fixed, then dyed with the red solution of tiger.Sample after freezing, processing Shi Xianyong alcohol (70% concentration and with
On) fixed, then dyed with the red solution of tiger.
2, crystallising dish or beaker are numbered and are weighed
It takes crystallising dish clear water to clean, is dried in 60 DEG C of drying boxes.Crystallising dish is numbered and is weighed and (is accurate to
0.01g), the weight of record crystallising dish and corresponding sample number in sample table, fill in and rush sample record sheet.
3, sample drying weighing
It takes primary deposit object sample to be put into the crystallising dish for having weighed weight, and record number, weighs deposit weight in wet base, it will
Sediment sample is dried completely in 60 DEG C of drying boxes, and is weighed, and the dry weight for obtaining sample is calculated.
4, it impregnates and disperses
The halmeic deposit of drying is taken, if the sample content of organic matter is lower, distilled water or tap water is added, sufficiently impregnates (1
It~2 days) it is completely dispersed sample.The high sample of the content of organic matter was made with hydrogen peroxide dipping sample 2 hours that concentration is 3%
Sediment sample is completely dispersed.
5, " sand hill " rushes sample and dries
Deposit is imported in the bolting silk that aperture is 300 mesh and is made " sand hill ", " sand hill " is teetertottered in clear water,
Silt exchanges rapidly with water, and water is then changed after water turbidity and repeats the step, until water quality is limpid, then sample wash is clean.Transfer
The sample rinsed well is into crystallising dish and numbers, and crystallising dish is dried in 60 DEG C of insulating boxs.Samples weighing after drying is simultaneously
It is recorded as thick composition weight.
6, flotation is concentrated
Large beaker number is corresponding with sample number, is suspended using carbon tetrachloride.Carbon tetrachloride is poured into beaker, then
Thick component sample is uniformly sprinkling upon in carbon tetrachloride, is filtered with the sieve of 0.063mm, after repeated filtration several times, is obtained
Sample be purifying sediment sample.After sample after suspending is completely dried, deposit residue is poured into crystallising dish simultaneously
Remaining deposit residue is packed and marks erect-position by number, crystallising dish microscopy.
7, it is sieved and bottles and save
Drying sample after weighing is crossed into 0.15mm mesh screen respectively and 0.063mm mesh screen, two groups of samples are bottled and pasted respectively
Upper label indicates sample layer position and shell diameter information.
8, microsection and analysis
The sample that will be greater than 0.15mm carries out microscopy under the microscope, selects 150~300 shell samples at random
Piece.According to the needs of task, taxonomic identification and group's statistics (including kind of number, abundance, point different degree and each monoid ratio are carried out
Deng).According to the requirement of task, may be selected to carry out carrying out identification statistics to the sample of 0.063mm.Fill in foraminifer identification system
Count table.
Claims (5)
1. a kind of microbody sample of deep-sea foraminifer quantitatively purifies and production method, it is characterised in that: halmeic deposit will be acquired
It is dyed through fixed, then sediment sample is taken to dry, then impregnated and dispersed, it is 300 purposes that sample, which is transferred to aperture,
" sand hill " is made in bolting silk；It rinses " sand hill " and dries again, the sample in " sand hill " is subjected to flotation concentration, sieving, it is micro-
Film-making can be obtained the microbody sample of deep-sea foraminifer after purification.
2. the microbody sample of deep-sea foraminifer according to claim 1 quantitatively purifies and production method, which is characterized in that
The halmeic deposit of acquisition is fixed through alcohol, is dyed with the red solution of tiger, through electric heating forced air drying after dyeing is fixed
Case is dried, and halmeic deposit is impregnated and dispersed with hydrogen peroxide after drying and processing, and it is 300 that sample, which is transferred to aperture,
" sand hill " is made in purpose bolting silk, " sand hill " is rinsed and dries again, it is dense then to carry out flotation to sample using carbon tetrachloride
Contracting, sieving, microsection can be obtained the microbody sample of deep-sea foraminifer after purification.
3. the microbody sample of the deep-sea foraminifer according to claims 1 or 2 quantitatively purifies and production method, feature exist
In the experimentation of sample wash and foraminifer drying carries out in the bolting silk that aperture is 300 mesh, then filtration treatment.
4. the microbody sample of the deep-sea foraminifer according to claims 1 or 2 quantitatively purifies and production method, feature exist
In, sample after soaking dispersion is placed in the bolting silk that aperture is 300 mesh, " sand hill " is made through bolting silk package, it then will be " husky
Packet " is rinsed together with samples with water.
5. the microbody sample of the deep-sea foraminifer according to claims 1 or 2 quantitatively purifies and production method, feature exist
In the sieve using 0.15mm and 0.063mm carries out sieving separating processing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711430803.0A CN109959540A (en) | 2017-12-26 | 2017-12-26 | The microbody sample of deep-sea foraminifer quantitatively purifies and production method |
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| CN201711430803.0A CN109959540A (en) | 2017-12-26 | 2017-12-26 | The microbody sample of deep-sea foraminifer quantitatively purifies and production method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110491266A (en) * | 2019-08-26 | 2019-11-22 | 山东小巨人海洋科技有限公司 | A kind of experimental courses for finding foraminifer |
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| DE102012215628A1 (en) * | 2012-09-04 | 2014-03-06 | Leibniz-Institut für Ostseeforschung Warnemünde | Device for sample taking of soft sediments with sample tube, has lifting eye arranged as suspension, which is operatively connected to units for closing upper- and lower ends of sample tube through locking unit |
| US9285300B2 (en) * | 2012-09-19 | 2016-03-15 | Gl Sciences Incorporated | Monolithic silicone and method of separation, purification and concentration therewith |
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2017
- 2017-12-26 CN CN201711430803.0A patent/CN109959540A/en active Pending
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| CN1762612A (en) * | 2005-11-11 | 2006-04-26 | 国家海洋局第二海洋研究所 | Automatic separation system of small sized benthos specimen |
| DE102012215628A1 (en) * | 2012-09-04 | 2014-03-06 | Leibniz-Institut für Ostseeforschung Warnemünde | Device for sample taking of soft sediments with sample tube, has lifting eye arranged as suspension, which is operatively connected to units for closing upper- and lower ends of sample tube through locking unit |
| US9285300B2 (en) * | 2012-09-19 | 2016-03-15 | Gl Sciences Incorporated | Monolithic silicone and method of separation, purification and concentration therewith |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110491266A (en) * | 2019-08-26 | 2019-11-22 | 山东小巨人海洋科技有限公司 | A kind of experimental courses for finding foraminifer |
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