CN100421578C - Method for preparing water soluble royal jelly - Google Patents
Method for preparing water soluble royal jelly Download PDFInfo
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- CN100421578C CN100421578C CNB2006100525397A CN200610052539A CN100421578C CN 100421578 C CN100421578 C CN 100421578C CN B2006100525397 A CNB2006100525397 A CN B2006100525397A CN 200610052539 A CN200610052539 A CN 200610052539A CN 100421578 C CN100421578 C CN 100421578C
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- Prior art keywords
- royal jelly
- water
- water soluble
- vacuum
- enzymolysis
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- 229940109850 royal jelly Drugs 0.000 title claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims description 6
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000002061 vacuum sublimation Methods 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 4
- 239000013078 crystal Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
- 238000009461 vacuum packaging Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000002932 luster Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000013081 microcrystal Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- WXBXVVIUZANZAU-CMDGGOBGSA-N trans-2-decenoic acid Chemical compound CCCCCCC\C=C\C(O)=O WXBXVVIUZANZAU-CMDGGOBGSA-N 0.000 description 1
Landscapes
- Jellies, Jams, And Syrups (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The preparation process of water soluble royal jelly includes the following steps: defreezing royal jelly at room temperature; homogenizing via adding water in 1.5-3 times and stirring; enzymolyzing with proteinase at 40-45deg.c while stirring for 2-4 hr and ultrafiltering membrane or vacuum concentration at normal temperature to the amount of 1-1.5 times of royal jelly material; freeze drying; and vacuum packing. The water soluble royal jelly product has water content not more than 3 wt% and 10-HAD content not less than 4.0 wt%. The preparation process is simple and reliable, and the water soluble royal jelly product has wide dissolving temperature range, high stability, water dissolving retention rate as high as 90 % and other features.
Description
Technical field
That the present invention relates to is a kind of preparation method of water soluble royal jelly, belongs to the medicine food preparation field.
Background technology
On the Chinese patent communique, disclose a kind of process for preparing transparent royal jelly solution patent application technology, number of patent application is CN92110352.2, it provides a kind of manufacturing process easy and cheap transparent and stable royal jelly solution manufacture method, wherein modulate the royal jelly aqueous suspension, to wherein simultaneously or add two kind or more the protease different successively to the matrix site of action, temperature keeps by enzyme reaction insoluble matter can being dissolved more than the room temperature.But this patent application technology uses complex enzyme to make process complications, thereby production cost increases, and differs greatly with the local flavor of original royal jelly.
Summary of the invention
The object of the present invention is to provide a kind of royal jelly that will make can be solvable in water, do not lose original local flavor again, comprise and detect index 10-hydroxyl-the 2-decylenic acid is qualified, and the preparation method of the water soluble royal jelly that can produce in enormous quantities.This method comprises: 1. thaw, royal jelly is at room temperature thawed; 2. even matter adds 1.5～3 times of water gagings, stirs; 3. enzymolysis adds protease, and 40～45 ℃ are stirred enzymolysis and extraction 2～4h, and the normal temperature milipore filter concentrates or vacuum is concentrated into 1～1.5 times of former royal jelly input amount; 4. freeze drying; 5. vacuum-packed.
The present invention returns to fluid state after royal jelly is thawed; When sparing matter, also carry out the even matter of high speed shear, grain fineness≤20 μ m; Refinement decylenic acid crystal makes its commentaries on classics water-soluble, changes molten rate 〉=95%.
The solid content of the present invention's gained after carrying out enzymolysis reaches the royal jelly total solid matters more than 95%; When freeze drying, fast-refrigerating is to-40～60 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HAD content 〉=4.0%.
The present invention utilizes normal temperature film concentration technology, a milipore filter permeate water, and full technological work temperature≤45 ℃ is avoided the influence of high temperature to temperature sensitive actives matter in the royal jelly.Goods dissolving adaptive temperature scope is wide: 1～90 ℃; Solubility in the water 〉=10% (20 ℃), solution clear, good stability; 10-hydroxyl-2-decylenic acid (10-HDA) is the main active ingredient of royal jelly, indissoluble and water, and ultra-fineization of this process using high speed shear decylenic acid crystal increases solubility, and the retention rate after the water-soluble conversion is up to more than 90%; Adopt fast, cross freezing knot and generate lyophilization behind the microcrystal, keep the particulate state of 10-HDA, 10-HDA keeps high stripping state after the finished product rehydration.
The specific embodiment
The present invention will be described in detail below in conjunction with specific embodiment: preparation method of the present invention is: 1. royal jelly room temperature (20～35 ℃) is thawed: the back royal jelly that thaws returns to fluid state; 2. even matter: add 1.5～3 times of water gagings, stir, high speed shear is spared matter, grain fineness≤20 μ m; Refinement decylenic acid crystal makes its commentaries on classics water-soluble, changes molten rate 〉=95%; 3. enzymolysis: add multiple can the hydrolysis royal jelly in the enzyme (protease) of water-insoluble, 40～45 ℃ are stirred enzymolysis and extraction 2～4h; The solid content of gained reaches the royal jelly total solid matters more than 95%; 4. film concentrates: the normal temperature milipore filter is concentrated into 1～1.5 times of former royal jelly input amount, removes part moisture, avoids the influence of high temperature to temperature sensitive actives matter in the royal jelly simultaneously; 5. freeze drying: fast-refrigerating is to-40～60 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HAD content 〉=4.0%; Vacuum pack with nitrogen.
Embodiment 1. gets under the royal jelly room temperature that is up to the standards promptly 20～35 ℃ and thaws, and adds 1.5 times of water gagings, stirs, and high speed shear is spared matter, grain fineness≤20 μ m.Add protease, 40 ℃ are stirred enzymolysis 2h; Film is concentrated into 1 times of royal jelly input amount; Fast-refrigerating is to-40 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HDA content 〉=4.0%; Vacuum pack with nitrogen.
Embodiment 2. gets the royal jelly room temperature (20～35 ℃) that is up to the standards and thaws, and adds 2 times of water gagings, stirs, and high speed shear is spared matter, grain fineness≤20 μ m.Add protease, 42 ℃ are stirred enzymolysis 3h; Vacuum concentrates (60 ℃, 0.08MPa vacuum) 1.3 times to the royal jelly input amount; Fast-refrigerating is to-50 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HDA content 〉=4.0%; Vacuum pack with nitrogen.
Obviously deepen with the goods comparison color and luster of embodiment 1, the finished product color and luster of embodiment 1 is the same with common royal jelly freeze-dried powder color and luster to be pale yellow powder, and embodiment 2 is a light brown powder.
Embodiment 3. gets the royal jelly room temperature (20～35 ℃) that is up to the standards and thaws, and adds 3 times of water gagings, stirs, and adds protease, and 45 ℃ are stirred enzymolysis 4h; Film is concentrated into 1.5 times of royal jelly input amount; Fast-refrigerating is to-60 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HDA content≤3.0%; Vacuum pack with nitrogen.
Compare the decline of 10-HAD content obviously with the goods of embodiment 1,2.
Claims (2)
1. the preparation method of a water soluble royal jelly, this method comprises: 1. thaw, royal jelly is at room temperature thawed, the royal jelly after thawing returns to fluid state; 2. even matter adds 1.5～3 times of water gagings, stirs, and also carries out the even matter of high speed shear, grain fineness≤20 μ m; Refinement decylenic acid crystal makes its commentaries on classics water-soluble, changes molten rate 〉=95%; 3. enzymolysis adds protease, and 40～45 ℃ are stirred enzymolysis and extraction 2～4h, and the normal temperature milipore filter concentrates or vacuum is concentrated into 1～1.5 times of former royal jelly input amount; 4. freeze drying; 5. vacuum-packed.
2. the preparation method of a kind of water soluble royal jelly according to claim 1 is characterized in that the solid content of gained after carrying out enzymolysis reaches the royal jelly total solid matters more than 95%; When freeze drying, fast-refrigerating is to-40～60 ℃, freeze-off time≤30 minute, vacuum sublimation drying, goods moisture≤3%, 10-HAD content 〉=4.0%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100525397A CN100421578C (en) | 2006-07-18 | 2006-07-18 | Method for preparing water soluble royal jelly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100525397A CN100421578C (en) | 2006-07-18 | 2006-07-18 | Method for preparing water soluble royal jelly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1899093A CN1899093A (en) | 2007-01-24 |
| CN100421578C true CN100421578C (en) | 2008-10-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100525397A Active CN100421578C (en) | 2006-07-18 | 2006-07-18 | Method for preparing water soluble royal jelly |
Country Status (1)
| Country | Link |
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| CN (1) | CN100421578C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129410B (en) * | 2007-08-01 | 2012-05-02 | 浙江大学 | Royal jelly enzymolysis product with moisture-absorbing moisture-keeping function |
| CN102302108A (en) * | 2011-07-10 | 2012-01-04 | 荆门市顺昌蜂业有限公司 | Pure royal jelly tablet machining process |
| CN106879884B (en) * | 2015-12-15 | 2020-09-25 | 中国农业大学 | Method for improving royal jelly performance |
| CN105660888B (en) * | 2016-01-20 | 2019-11-12 | 福建天斯顿生物科技有限公司 | A kind of camellia oil compound product and preparation method thereof |
| CN106215068A (en) * | 2016-08-25 | 2016-12-14 | 明光市昊昊蜂业有限公司 | A kind of defying age FENGWANGJIANG KOUFUYE |
| CN108669509A (en) * | 2018-03-21 | 2018-10-19 | 巢氏健康生物科技股份有限公司 | A kind of novel royal jelly liquid and preparation method thereof containing 10-HDA liposomes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070804A (en) * | 1991-09-09 | 1993-04-14 | 拜奥斯有限公司 | Process for preparing transparent royal jelly solution |
| CN1561860A (en) * | 2004-04-02 | 2005-01-12 | 戚向阳 | Bee royal jelly health drink and its preparing method |
-
2006
- 2006-07-18 CN CNB2006100525397A patent/CN100421578C/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1070804A (en) * | 1991-09-09 | 1993-04-14 | 拜奥斯有限公司 | Process for preparing transparent royal jelly solution |
| CN1561860A (en) * | 2004-04-02 | 2005-01-12 | 戚向阳 | Bee royal jelly health drink and its preparing method |
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| Publication number | Publication date |
|---|---|
| CN1899093A (en) | 2007-01-24 |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Hangzhou Aoyi Baoling Pharmaceutical Co., Ltd. Assignor: Hangzhou Baoling Co., Ltd. Contract fulfillment period: 2008.8.6 to 2026.7.18 Contract record no.: 2008330002017 Denomination of invention: Method for preparing water soluble royal jelly Granted publication date: 20081001 License type: Exclusive license Record date: 20081114 |
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| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.8.6 TO 2026.7.18; CHANGE OF CONTRACT Name of requester: HANGZHOU AOYI BAOLING PHARMACEUTICAL CO., LTD. Effective date: 20081114 |
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| C56 | Change in the name or address of the patentee |
Owner name: HANGZHOU BAOLING GROUP CO.,LTD. Free format text: FORMER NAME: HANGZHOU BAO LING CO., LTD. |
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Address after: Hangzhou City, Zhejiang Province, Gongshu District mid Baolinglu No. 5 Patentee after: Hangzhou Pollen Group Co.,Ltd. Address before: Hangzhou City, Zhejiang Province, Gongshu District mid Baolinglu No. 5 Patentee before: Hangzhou Baoling Co., Ltd. |
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| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Hangzhou Aoyi Baoling Pharmaceutical Co., Ltd. Assignor: Hangzhou Baoling Co., Ltd. Contract record no.: 2008330002017 Date of cancellation: 20160525 |
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| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
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Effective date of registration: 20160620 Address after: 310000 No. 23, No. 668, Hangzhou economic and Technological Development Zone, Hangzhou, Zhejiang Patentee after: Hangzhou Aoyi Baoling Pharmaceutical Co., Ltd. Address before: Hangzhou City, Zhejiang province 310022 Gongshu District mid Baolinglu No. 5 Patentee before: Hangzhou Pollen Group Co.,Ltd. |
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Address after: 310018 no.668, No.23 street, Hangzhou Economic and Technological Development Zone, Hangzhou, Zhejiang Province Patentee after: Hangzhou Qianyuan Baoling Pharmaceutical Co., Ltd Address before: 310000 No. 23, No. 668, Hangzhou economic and Technological Development Zone, Hangzhou, Zhejiang Patentee before: HANGZHOU AOYIPOLLEN PHARMACEUTICAL Co.,Ltd. |
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