CN100375632C

CN100375632C - Extractive from combined vegetable drug for treating heart disease of coronary sclerosis, and method of extraction

Info

Publication number: CN100375632C
Application number: CNB2004100417336A
Authority: CN
Inventors: 戴翔翎; 凌娅; 李明慧; 丁岗
Original assignee: Jiangsu Zeukov Pharmaceutical S & T Inc
Current assignee: Jiangsu Kanion Pharmaceutical Co Ltd
Priority date: 2004-08-15
Filing date: 2004-08-15
Publication date: 2008-03-19
Anticipated expiration: 2024-08-15
Also published as: CN1626216A

Abstract

The present invention relates to an extract of combined vegetable medicine for curing coronary heart diseases and a method of extraction. Raw materials contain red sage root, red ginseng, dwarf lilyturf root and schisandra. After the schisandra and the red sage root are extracted by water, alcohol is used for extraction. The red ginseng and the dwarf lilyturf root are extracted by the alcohol and are extracted by normal butyl alcohol. Distillation is matched, and macroporous resin is used for sorption extraction. Extracts are combined to obtain the extract of combined vegetable medicine. An oral preparation is prepared from the extract and contains tablets, capsules, soft capsules, drop pills, granules and oral liquid. After the extract is processed by active carbons and pH values, an injection is prepared. The present invention is extracted by a strict modernization method. By medicine, virus theories, clinical tests and modern methods, effective contents and heavy metals are detected. Compared with traditional prescriptions, the present invention is the new medicine of traditional Chinese medicine with the advantages of stable and controllable quality, high safety, good effects, high efficiency, quick effects and few toxic side effects. By experimental verification, the extract of the combined vegetable medicine of the present invention and a preparation prepared by different extraction methods have exact curative effects on curing coronary heart diseases, myocardial ischemia, etc.

Description

The combined plant medicament extract and the extracting method of treatment coronary atherosclerotic heart disease

Technical field

The present invention relates to combined plant medicament extract and extracting method, particularly treat the combined plant medicament extract and the extracting method of coronary atherosclerotic heart disease.

Background technology

Coronary heart disease is exactly that tangible medicated porridge shape sclerostenosis or obstruction take place coronary artery, or merges spasm or thrombosis on this basis, and causes tube chamber partly or entirely to block, and causes coronary insufficiency, and myocardial ischemia is so that infraction is downright bad.These a series of pathological changes just are referred to as coronary heart disease.Full name is a coronary atherosclerotic heart disease.Evidence of coronary heart diseases increases in recent years year by year.Become one of principal disease of harm middle-aged and elderly people health, serious threat people life and influence quality of life, its sickness rate and mortality rate are all in rising year by year.Pain management when the treatment of coronary heart disease mainly comprises angina pectoris attacks and prophylactic treatment at ordinary times.Based on prophylactic treatment.The treatment of coronary heart disease must combine with prophylactic treatment by pain management, and is anxious then treat its mark, radical treatment in chronic case.Pain management during outbreak contains with nitroglycerin or sorbitrate based on the Sublingual.And prophylactic treatment comprises Drug therapy, operative treatment and exercise therapy.Drug therapy comprises Western medicine and Chinese medicine, and Western medicine is to resist myocardial ischemia, the prevention angina pectoris.Stage of attack, use Western medicine better, and the mild phase should rationally be selected Chinese medicine for use.Chinese traditional treatment has at present obtained than ten-strike, has alleviated the patient suffering greatly, has reduced mortality rate, has improved patients ' life quality, has prolonged the patient and has survived the life-span.

The prescription of existing treatment by Chinese herbs coronary atherosclerotic heart disease derives from traditional Chinese herb drugs prescription, and main is Radix Salviae Miltiorrhizae, Radix Ginseng Rubra, Radix Ophiopogonis effectively, also can add Fructus Schisandrae Chinensis.Its weak point is: often because of the difference in the place of production, season, its active ingredient instability causes curative effect influenced to the raw material of traditional Chinese herb drugs prescription or traditional Chinese patent medicine; Moreover, be subjected to the pollution composition of modern chemistry product and unfavorable composition to bring in the patient body easily in its raw material; And it is extremely inconvenient during patient's medication.

Summary of the invention

The objective of the invention is at the deficiencies in the prior art, grope,, proposed the extract and the extracting method thereof of the combined plant medicine of treatment coronary atherosclerotic heart disease in conjunction with advanced modern extraction process according to clinical experience for many years.

The objective of the invention is to be reached by following technical scheme, the present invention is a kind of extract for the treatment of the combined plant medicine of coronary atherosclerotic heart disease, be characterized in that the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 80～120 weight portion Radix Ginseng Rubra 80～120 weight portions

Radix Ophiopogonis 250～375 weight portion Fructus Schisandrae Chinensis, 0～160 weight portion

Weight portion described in the present invention with corresponding relation between the parts by volume is: corresponding 1 ml volumes of 1 gram weight;

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 2～4 times, each 4～8 times of water gagings decocted 1～2 hour at every turn, collecting decoction filters, and filtrate is concentrated into 650～850 parts, add ethanol and make and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol is concentrated into 250～400 parts by volume, adds ethanol again and makes and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(b) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials become coarse powder, conflux with 45%～75% ethanol of 4～8 times of amounts and to extract 2～3 times, each 1～2 hour, merge extractive liquid,, cold preservation 12～48 hours, filter, filtrate recycling ethanol is to not having alcohol flavor, cold preservation 12～48 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 3～6 times, or with 3～15 times of water-saturated n-butanol counter-current extractions, merge n-butanol extracting liquid, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(c) merge the extract that Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract and Radix Ginseng Rubra and Radix Ophiopogonis extract get the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Another object of the present invention is reached by following technical scheme, the present invention is a kind of extracting method of extract of combined plant medicine for the treatment of coronary atherosclerotic heart disease, is characterized in that this method is meant the prescription described in the technique scheme and (a) and (b), (c) step method.

Another object of the present invention also can be reached by following technical scheme, and the present invention is a kind of extracting method of extract of combined plant medicine for the treatment of coronary atherosclerotic heart disease, is characterized in that the formulation by weight of combined plant medicament extract raw material is:

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 2～4 times, each 4～8 times of water gagings decocted 1～2 hour at every turn, collecting decoction filters, and filtrate is concentrated into 650～850 parts, add ethanol and make and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol, be concentrated into 250～400 parts by volume, add ethanol again and make and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, in solution, by volume add 0.8～1.2% active carbon, stirring is no less than 1 hour, filters filtrate recycling ethanol, be concentrated into 200～330 parts by volume, add 4～5 times of waters for injection, leave standstill more than 12 hours, filter, filtrate is concentrated into 300～380 parts by volume, add ethanol and make and contain alcohol amount and reach 75～85%, leave standstill and be no less than 24 hours, filter, filtrate is concentrated into 300～380 parts by volume, gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract;

(c) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add injection water 160～240 parts by volume, stir and make its dissolving, filter; Add the active carbon heated and stirred by 0.5～2.0% of filtrate weight, adsorbed 20～40 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract, add injection water to 1000 parts by volume, transfer pH value to 7.0～8.0 with 40% sodium hydroxide NaOH, add 0.5～2.0 parts by volume sodium sulfite, add caffolding agent 50～200 parts by volume, dissolving, filtration, the classification ultrafiltration is collected ultrafiltrate, fill, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

(h) red ginseng powder is broken into particulate, uses alcohol reflux 4～5 times, each 1.5～2.5 hours, merge extractive liquid,, cold preservation filters, and the filtrate concentrate drying gets Radix Ginseng Rubra extract;

(i) Radix Salviae Miltiorrhizae decocts with water 2～4 times, adds 4～8 times of water gagings at every turn, decocts 1～2 hour, collecting decoction filters, and filtrate is concentrated into 650～850 parts by volume, add ethanol and make and contain the alcohol amount and reach 70～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol is concentrated into 250～400 parts by volume, adds ethanol again and makes and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

(i) Radix Ophiopogonis and Fructus Schisandrae Chinensis decoct with water 2～4 times, and each 30～50 minutes, 4～8 times of each amount of water, collecting decoction, filter, filtrate is concentrated into the thick paste shape, adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and concentrate drying, get Radix Ophiopogonis and Fructus Schisandrae Chinensis extrat;

(k) merge Radix Ginseng Rubra extract, Radix Salviae Miltiorrhizae extract, Radix Ophiopogonis and Fructus Schisandrae Chinensis extrat, get the extract of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

(p) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials become coarse powder, conflux with 45%～75% ethanol of 4～8 times of amounts and to extract 2～3 times, each 1～2 hour, merge extractive liquid,, cold preservation 12～48 hours, filter, filtrate recycling ethanol is to not having alcohol flavor, cold preservation 12～48 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 3～6 times, or with 3～15 times of water-saturated n-butanol counter-current extractions, merge n-butyl alcohol, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(q) Fructus Schisandrae Chinensis is collected distillate 120～180 parts by volume with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(r) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 2～4 times, each 30～50 minutes, each amount of water was 4～8 times of medicinal residues, collecting decoction, filter, relative density 1.05～1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 180～220 parts by volume, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 20～40 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(s) Radix Salviae Miltiorrhizae decocts with water 2～4 times, and each amount of water is 4～8 times, decocting time 1～2 hour, collecting decoction filters, and filtrate is concentrated into 600～850 parts by volume, add ethanol and make and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol, be concentrated into 250～400 parts by volume, add ethanol again and make and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, in solution, by volume add 0.8～1.2% active carbon, stirring is no less than 1 hour, filters filtrate recycling ethanol, be concentrated into 200～330 parts by volume, add 4～5 times of waters for injection, leave standstill and be no less than 12 hours, filter, filtrate is concentrated into 300～380 parts by volume, add ethanol and make and contain alcohol amount and reach 75～85%, leave standstill and be no less than 24 hours, filter, filtrate is concentrated into 300～380 parts by volume, gets Radix Salviae Miltiorrhizae extract;

(t) add water for injection 100～150 parts by volume, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 2～20 weight portion active carbon heated and stirred absorption 20～40 minutes, and is cold slightly, filters, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700～900 parts by volume, the sodium hydroxide NaOH with 8～12% transfers pH value to 6.5～8.0, adds antioxidant 0.5～2.0 parts by volume, dissolving, filter, ultrafiltrate is collected in the classification ultrafiltration, adds Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960～1040 parts by volume, embedding is sterilized, and promptly gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

(u) red ginseng powder is broken into particulate, uses alcohol reflux 4～5 times, each 1.5～2.5 hours, merge extractive liquid,, cold preservation filters, and filtrate is concentrated into the thick paste shape, adds injection water to 360～440 parts by volume,, stirring evenly, cold preservation filters, and gets Radix Ginseng Rubra extract;

(v) decoct with water Radix Ophiopogonis 2～4 times, each 30～50 minutes, each amount of water was 4～8 times of medicinal residues, collecting decoction, filter, relative density 1.05～1.15 when filtrate is concentrated into 50 ℃ of heat surveys adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and be concentrated into the thick paste shape, add injection water to 180～220 parts by volume,, stir evenly, cold preservation, filter, filtrate adds activated carbon boiled 20～40 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(w) Fructus Schisandrae Chinensis is collected distillate 120～180 parts by volume with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(x) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 2～4 times, each 30～50 minutes, each amount of water was 4～8 times of medicinal residues, collecting decoction, filter, relative density 1.05～1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 180～220 parts by volume, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 20～40 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(y) Radix Salviae Miltiorrhizae decocts with water 2～4 times, and each amount of water is 4～8 times, decocting time 1～2 hour, collecting decoction filters, and filtrate is concentrated into 600～850 parts by volume, add ethanol and make and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol, be concentrated into 250～400 parts by volume, add ethanol again and make and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, in solution, by volume add 0.8～1.2% active carbon, stirring is no less than 1 hour, filters filtrate recycling ethanol, be concentrated into 200～330 parts by volume, add 4～5 times of waters for injection, leave standstill and be no less than 12 hours, filter, filtrate is concentrated into 300～380 parts by volume, add ethanol and make and contain alcohol amount and reach 75～85%, leave standstill and be no less than 24 hours, filter, filtrate is concentrated into 300～380 parts by volume, gets Radix Salviae Miltiorrhizae extract;

(z) get Radix Ginseng Rubra extract, add active carbon heated and stirred absorption 20～40 minutes by 0.5～2.0% of filtrate weight, cold slightly, filter, add Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700～900 parts by volume, the sodium hydroxide NaOH with 8～12% transfers pH value to 6.5～8.0, adds antioxidant 0.5～2.0 parts by volume, dissolving, filter, ultrafiltrate is collected in the classification ultrafiltration, adds Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960～1040 parts by volume, embedding is sterilized, and promptly gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

(α) red ginseng powder is broken into particulate, uses alcohol reflux 4～5 times, each 1.5～2.5 hours, merge extractive liquid,, cold preservation filters, and filtrate is concentrated into the thick paste shape, adds injection water to 360～440 parts by volume,, stirring evenly, cold preservation filters, and gets Radix Ginseng Rubra extract;

(β) decoct with water 2～4 Radix Ophiopogonis, each 30～50 minutes, each amount of water was 4～8 times of medicinal residues, collecting decoction, filter, relative density 1.05～1.15 when filtrate is concentrated into 50 ℃ of heat surveys adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and be concentrated into the thick paste shape, add injection water to 180～220 parts by volume,, stir evenly, cold preservation, filter, filtrate adds activated carbon boiled 20～40 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(γ) with Fructus Schisandrae Chinensis with Radix Salviae Miltiorrhizae extract Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract, that is: Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae decoct with water 2～4 times, and each amount of water is 4～8 times, decocting time 1～2 hour, collecting decoction, filter, filtrate is concentrated into 600～850 parts by volume, adds ethanol and makes and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol is concentrated into 250～400 parts by volume, adding ethanol again makes and contains alcohol amount and reach 80～90%, left standstill 24～48 hours, and by volume added 0.8～1.2% active carbon in solution, stirring is no less than 1 hour, filter, filtrate recycling ethanol is concentrated into 200～330 parts by volume, adds 4～5 times of waters for injection, leave standstill and be no less than 12 hours, filter, filtrate is concentrated into 300～380 parts by volume, adds ethanol and makes and contain the alcohol amount and reach 75～85%, leave standstill and be no less than 24 hours, filter, filtrate is concentrated into 300～380 parts by volume, gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(δ) merge Radix Ginseng Rubra extract, Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract, add injection water to 800～1000 parts by volume, add 2～20 weight portion active carbon heated and stirred absorption 20～40 minutes, cold slightly, filter, add Radix Ophiopogonis extract and water for injection to 1000 parts by volume, transfer pH value to 7.0～8.0 with 40% sodium hydroxide NaOH, add 0.5～2.0 parts by volume sodium sulfite, add caffolding agent 50～200 parts by volume, dissolving, filter, the classification ultrafiltration is collected ultrafiltrate, fill, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Another object of the present invention also can be reached by following technical scheme, and the present invention is a kind of extracting method of extract of combined plant medicine for the treatment of coronary atherosclerotic heart disease, be characterized in aforementioned technical scheme,

For (b) and (p) step, at (b), (p) in the step, change into and Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 0.8～1.2 gram medical material, centrifugal, extracting centrifugal liquid passes through macroporous resin adsorption, to be no less than 3 times, preferably the water elution of 4～6 times macroporous resin column volume discards water lotion, with 20%～30% ethanol elution, its 20%～30% ethanol preferable amount is 2～3 times of amount column volume amounts, discards eluent, again with 40%～70% ethanol elution, 40%～70% ethanol preferable amount is 15～20 times of amount column volumes, collect eluent, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract;

For other steps, identical with the aforementioned techniques scheme.

Purpose of the present invention is further reached by following technical scheme, extracting method according to the extract of aforesaid combined plant medicine, be characterized in that described macroporous resin column model is a kind of or its combination among D-101, D-201, AB-8, HPD-100, the NK-9, is preferably D-101.

Purpose of the present invention is further reached by following technical scheme, and the extracting method according to the extract of aforesaid combined plant medicine is characterized in that described antioxidant is sodium sulfite or sodium sulfite or sodium pyrosulfite or its combination, preferably sodium sulfite.

Purpose of the present invention is further reached by following technical scheme, according to the extracting method of the extract of aforesaid combined plant medicine, is characterized in the preferred film molecular cut off of described classification ultrafiltration: dalton, dalton for the second time≤30,000 for the first time≤100,000.

Purpose of the present invention is further reached by following technical scheme, and the extracting method according to the extract of aforesaid combined plant medicine is characterized in that described caffolding agent is mannitol or lactose or hydroxy propyl-Beta cyclodextrin or its combination, preferred mannitol.

Purpose of the present invention is further reached by following technical scheme, extracting method according to the extract of aforesaid combined plant medicine, be characterized in extract with the combined plant medicine of described treatment coronary atherosclerotic heart disease, make the described oral formulations of pharmaceutics, comprise tablet, capsule, soft capsule, drop pill, granule, oral liquid.

The extract of Chinese herbal medicine combined plant medicine of the present invention, the active ingredient through strict modernization extraction means have been extracted Chinese herbal medicine combined plant medicine discards invalid part.Through medicine, toxicity, clinical trial and modern means effective content and heavy metal are detected, stable and controllable for quality, safe and effective; Curative effect is also relatively stable; Side effect is little; Make various preparations,, very convenient during patient's medication.Have supplementing QI to prevent collapse, YIN nourishing and the production of body fluid promoting, give birth to the effect of arteries and veins, aspect the treatment coronary heart disease definite curative effect is being arranged, and having the immune effect of adjusting, can the nutrition heart, improve heart blood supply; The mediation vessel softening reduces heart labor intensity; Stable heart-rate, lightening heart load.For new Chinese medicines that disease provides efficient, quick-acting, toxic and side effects is little such as nervously breathing hard of causing of coronary heart disease, uncomfortable in chest, weak, cough and asthma, limbs edema, sleep shakinesses, has better curative effect.More help integral body execute control, the therapy of treatment must aim at the pathogenesis of disease,

Experiment showed, the extract of combined plant medicine of the present invention and the preparation that each extracting method makes, definite curative effect is arranged at aspects such as treatment coronary heart disease, myocardial ischemia.Be embodied in following aspect:

1, the preparation that makes of the extract of combined plant medicine of the present invention and each extracting method can obviously reduce the positive incidence rate of coronary heart disease rat heart muscle ischemia, reduces the model blood fat, thereby the coronary heart disease rat is played significant protective effect.

2, the preparation that makes of the extract of combined plant medicine of the present invention and each extracting method, can obviously reduce the total peripheral vascular resistance of anesthetized dog, reduce chamber, anesthetized dog left side EDP, increase the anesthetized dog coronary artery blood flow, shorten the anesthetized dog left ventricular ejection time, shorten the total oxygen consumption index of anesthetized dog.

3, the preparation that makes of the extract of combined plant medicine of the present invention and each extracting method has the anesthetized dog acute myocardial ischemia obviously to alleviate degree of myocardial ischemia, reduces the myocardial ischemia scope, reduces the effect of myocardial infarct size.

The specific embodiment

Be embodiments of the invention below, further described summary of the invention of the present invention.

Embodiment one: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

The formulation by weight of its combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 3 times, add 8 times of water gagings for the first time, decocted 2 hours, second and third time respectively adds 6 times, 5 times water gagings, the each decoction 1.5 hours, collecting decoction filters, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, leaves standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 330 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, filter, filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(b) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 60% ethanol of 6 times of amounts, each 1.5 hours, merge extractive liquid,, cold preservation 40 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 40 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 5 times, or with 3 times of water-saturated n-butanol counter-current extractions, merge n-butanol extracting liquid, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

Embodiment two: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 120 gram Radix Ginseng Rubra 120 gram grams Radix Ophiopogonis 250

(a) Radix Salviae Miltiorrhizae decocts with water 4 times, adds 4 times of water gagings at every turn, decocts 1 hour at every turn, collecting decoction filters, and filtrate is concentrated into 650 milliliters, add ethanol and make and contain the alcohol amount and reach 60%, left standstill 24 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 90%, left standstill 48 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

(b) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 45% ethanol of 8 times of amounts, each 1 hour, merge extractive liquid,, cold preservation 48 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 12 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 3 times, or with 9 times of water-saturated n-butanol counter-current extractions, merge n-butanol extracting liquid, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(c) merge the extract that Radix Salviae Miltiorrhizae extract and Radix Ginseng Rubra and Radix Ophiopogonis extract get the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment three: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 80 gram Radix Ginseng Rubra 80 grams

Radix Ophiopogonis 375, gram Fructus Schisandrae Chinensis 160 restrained

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 2 times, add 8 times of water gagings at every turn, decocted 2 hours at every turn, collecting decoction filters, and filtrate is concentrated into 850 milliliters, add ethanol and make and contain the alcohol amount and reach 80%, left standstill 48 hours, filter, filtrate recycling ethanol is concentrated into 400 milliliters, adds ethanol again and makes and contain alcohol amount and reach 80%, left standstill 24 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(b) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 2 times of confluxing of 75% ethanol of 4 times of amounts, each 2 hours, merge extractive liquid,, cold preservation 12 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 48 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 6 times, or with 15 times of water-saturated n-butanol counter-current extractions, merge n-butanol extracting liquid, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

Embodiment four: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(b) Radix Ginseng Rubra, Radix Ophiopogonis two, the flavor pulverizing medicinal materials became coarse powder, with the extraction 2 times of confluxing of 75% ethanol of 4 times of amounts, each 2 hours, merge extractive liquid,, cold preservation 12 hours filters, and filtrate recycling ethanol is to there not being the alcohol flavor, cold preservation 48 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 0.8 and restrain medical material, centrifugal, extracting centrifugal liquid is by macroporous resin adsorption, and the water elution with 3 times macroporous resin column volumes discards water lotion, with 20% ethanol elution, discard eluent,, collect eluent again with 70% ethanol elution, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract;

Embodiment five: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 155 restrained

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 3 times, add 8 times of water gagings for the first time, decocted 2 hours, second, respectively add 6 times for three times, 5 times of water gagings decocted collecting decoction 1.5 hours at every turn, filter, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, left standstill 40 hours, and filtered filtrate recycling ethanol, be concentrated into 330 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, in solution, by volume add 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 300 milliliters, adds 4 times of waters for injection, left standstill 15 hours, and filtered, filtrate is concentrated into 300 milliliters, adding ethanol makes and contains alcohol amount and reach 80%, left standstill 24 hours, and filtered, filtrate is concentrated into 300 milliliters, filter, filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract;

(c) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 200 milliliters of injection waters, stir and make dissolving, filter; Add the active carbon heated and stirred by 1.2% of filtrate weight, adsorbed 30 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract, add injection water to 1000 milliliter, with 40% sodium hydroxide NaOH accent pH value to 7.5, add 1.0 gram sodium sulfitees, add 120 milliliters in caffolding agent mannitol, dissolving, filter the classification ultrafiltration, its retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate, fill for the second time≤30,000, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment six: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 155 restrained

(b) Radix Ginseng Rubra, Radix Ophiopogonis two, the flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 60% ethanol of 6 times of amounts, each 1.5 hours, merge extractive liquid,, cold preservation 40 hours filters, and filtrate recycling ethanol is to there not being the alcohol flavor, cold preservation 40 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 1 and restrain medical material, centrifugal, extracting centrifugal liquid is by macroporous resin adsorption, and the water elution with 5 times macroporous resin column volumes discards water lotion, 25% ethanol elution with 3 times of amount column volumes, discard eluent, with 55% ethanol elution of 18 times of amount column volumes, collect eluent again, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract; The model of the macroporous resin of present embodiment is D-101;

(c) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 160 milliliters of injection waters, stir and make dissolving, filter; Add the active carbon heated and stirred by 2.0% of filtrate weight, adsorbed 20 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract, add injection water to 1000 milliliter, with 40% sodium hydroxide NaOH accent pH value to 7.5, add 1.0 gram sodium sulfitees, add 120 milliliters in caffolding agent mannitol, dissolving, filter the classification ultrafiltration, its retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate, fill for the second time≤30,000, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment seven: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 80 gram Radix Ginseng Rubra 80 grams

Radix Ophiopogonis 375, gram Fructus Schisandrae Chinensis 160 restrained

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 3 times, add 8 times of water gagings for the first time, decocted 2 hours, second, respectively add 6 times for three times, 5 times of water gagings decocted collecting decoction 1.5 hours at every turn, filter, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, left standstill 40 hours, and filtered filtrate recycling ethanol, be concentrated into 330 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, in solution, by volume add 1.2% active carbon, stirred 1.5 hours, filter, filtrate recycling ethanol is concentrated into 330 milliliters, adds 5 times of waters for injection, left standstill 18 hours, and filtered, filtrate is concentrated into 380 milliliters, adding ethanol makes and contains alcohol amount and reach 85%, left standstill 30 hours, and filtered, filtrate is concentrated into 380 milliliters, filter, filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract;

(c) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 240 milliliters of injection waters, stir and make dissolving, filter; Add the active carbon heated and stirred by 0.5% of filtrate weight, adsorbed 40 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae liquid extract, add injection water to 1000 milliliter, with 40% sodium hydroxide NaOH accent pH value to 8, add 2.0 gram sodium sulfitees, add 200 milliliters of caffolding agent lactose, dissolving, filtration, classification ultrafiltration, its retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate, fill for the second time≤30,000, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment eight: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

(a) get Radix Salviae Miltiorrhizae and decoct with water 3 times, add 8 times of water gagings for the first time, decocted 2 hours, second, respectively add 6 times for three times, 5 times of water gagings decocted collecting decoction 1.5 hours at every turn, filter, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, left standstill 40 hours, and filtered filtrate recycling ethanol, be concentrated into 330 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, in solution, by volume add 0.8% active carbon, stirred 2 hours, filter, filtrate recycling ethanol is concentrated into 200 milliliters, adds 5 times of waters for injection, left standstill 12 hours, and filtered, filtrate is concentrated into 300 milliliters, adding ethanol makes and contains alcohol amount and reach 75%, left standstill 24 hours, and filtered, filtrate is concentrated into 300 milliliters, filter, filtrate recycling ethanol, drying under reduced pressure gets the Radix Salviae Miltiorrhizae liquid extract;

(b) identical with previous embodiment seven;

(c) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 160 milliliters of injection waters, stir and make dissolving, filter; Add the active carbon heated and stirred by 2.0% of filtrate weight, adsorbed 20 minutes, cold slightly, filter, add the Radix Salviae Miltiorrhizae liquid extract, add injection water to 1000 milliliter, with 40% sodium hydroxide NaOH accent pH value to 7, add 0.5 gram sodium sulfite, add 50 milliliters of caffolding agent hydroxy propyl-Beta cyclodextrin, dissolving, filter the classification ultrafiltration, its retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate, fill for the second time≤30,000, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment nine: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 145 restrained

(h) red ginseng powder is broken into particulate, uses alcohol reflux 5 times, each 2 hours, merge extractive liquid,, cold preservation filters, and the filtrate concentrate drying gets Radix Ginseng Rubra extract;

(i) Radix Salviae Miltiorrhizae decocts with water 3 times, adds 8 times of water gagings for the first time, decocts 2 hours, and second and third time respectively adds 6 times, 5 times water gagings, the each decoction 1.5 hours, collecting decoction filters, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, leaves standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 320 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, filter, filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

(i) Radix Ophiopogonis and Fructus Schisandrae Chinensis decoct with water 3 times, and each 40 minutes, 6 times of each amount of water, collecting decoction, filter, filtrate is concentrated into the thick paste shape, adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and concentrate drying, get Radix Ophiopogonis and Fructus Schisandrae Chinensis extrat;

Embodiment ten: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 110 gram Radix Ginseng Rubra 110 gram grams Radix Ophiopogonis 365

(h) red ginseng powder is broken into particulate, uses alcohol reflux 4 times, each 2.5 hours, merge extractive liquid,, cold preservation filters, and the filtrate concentrate drying gets Radix Ginseng Rubra extract;

(i) Radix Salviae Miltiorrhizae decocts with water 2 times, adds 8 times of water gagings at every turn, decocts 2 hours, collecting decoction filters, and filtrate is concentrated into 850 milliliters, add ethanol and make and contain the alcohol amount and reach 80%, left standstill 48 hours, filter, filtrate recycling ethanol is concentrated into 400 milliliters, adds ethanol again and makes and contain alcohol amount and reach 90%, left standstill 48 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

(j) decoct with water 2 Radix Ophiopogonis, each 50 minutes, 8 times of each amount of water, collecting decoction, filter, filtrate is concentrated into the thick paste shape, adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and concentrate drying, get Radix Ophiopogonis extract;

(k) merge Radix Ginseng Rubra extract, Radix Salviae Miltiorrhizae extract, Radix Ophiopogonis extract, get the extract of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 11: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of plant drug material is:

Radix Salviae Miltiorrhizae 90 gram Radix Ginseng Rubra 90 grams

Radix Ophiopogonis 300, gram Fructus Schisandrae Chinensis 130 restrained

(h) red ginseng powder is broken into particulate, uses alcohol reflux 5 times, each 1.5 hours, merge extractive liquid,, cold preservation filters, and the filtrate concentrate drying gets Radix Ginseng Rubra extract;

(i) Radix Salviae Miltiorrhizae decocts with water 4 times, adds 4 times of water gagings at every turn, decocts 1 hour, collecting decoction filters, and filtrate is concentrated into 650 milliliters, add ethanol and make and contain the alcohol amount and reach 70%, left standstill 24 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 80%, left standstill 24 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

(j) Radix Ophiopogonis and Fructus Schisandrae Chinensis decoct with water 4 times, and each 30 minutes, 4 times of each amount of water, collecting decoction, filter, filtrate is concentrated into the thick paste shape, adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and concentrate drying, get Radix Ophiopogonis and Fructus Schisandrae Chinensis extrat;

Embodiment 12: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(p) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 60% ethanol of 6 times of amounts, each 1.5 hours, merge extractive liquid,, cold preservation 40 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 40 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 5 times, or with 12 times of water-saturated n-butanol counter-current extractions, merge n-butyl alcohol, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(q) Fructus Schisandrae Chinensis is collected 150 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(r) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 3 times, each 40 minutes, each amount of water was 6 times of medicinal residues, collecting decoction, filter, relative density 1.1 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 200 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 30 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(s) Radix Salviae Miltiorrhizae decocts with water 3 times, and each amount of water is 6 times, decocting time 1.5 hours, collecting decoction filters, and filtrate is concentrated into 700 milliliters, add ethanol and make and contain the alcohol amount and reach 75%, left standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 340 milliliters, adds ethanol again and makes and contain alcohol amount and reach 85%, left standstill 40 hours, and in solution, by volume added 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 280 milliliters, add 4 times of waters for injection, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, add ethanol and make and contain alcohol amount and reach 80%, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, gets Radix Salviae Miltiorrhizae extract;

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 120 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 10 gram active carbon heated and stirred absorption 30 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 800 milliliter, sodium hydroxide NaOH with 10% transfers pH value to 7.3, add 1.2 milliliters of sodium sulfitees of antioxidant, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1000 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 13: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(p) Radix Ginseng Rubra, Radix Ophiopogonis two, the flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 60% ethanol of 6 times of amounts, each 1.5 hours, merge extractive liquid,, cold preservation 40 hours filters, and filtrate recycling ethanol is to there not being the alcohol flavor, cold preservation 40 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 1.2 and restrain medical materials, centrifugal, extracting centrifugal liquid is by macroporous resin adsorption, and the water elution with 6 times macroporous resin column volumes discards water lotion, 30% ethanol elution with 2 times of amount column volume amounts, discard eluent, with 20 times of amount column volume 40% ethanol elutions, collect eluent again, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract; The model of the macroporous resin of present embodiment is D-201;

Embodiment 14: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 90 gram Radix Ginseng Rubra 90 grams

Radix Ophiopogonis 300, gram Fructus Schisandrae Chinensis 130 restrained

(p) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 75% ethanol of 4 times of amounts, each 1 hour, merge extractive liquid,, cold preservation 12 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 12 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 3 times, or with 15 times of water-saturated n-butanol counter-current extractions, merge n-butyl alcohol, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(q) Fructus Schisandrae Chinensis is collected 120 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(r) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 2 times, each 50 minutes, each amount of water was 8 times of medicinal residues, collecting decoction, filter, relative density 1.05 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 180 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 20 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(s) Radix Salviae Miltiorrhizae decocts with water 2 times, and each amount of water is 8 times, decocting time 2 hours, collecting decoction filters, and filtrate is concentrated into 600 milliliters, add ethanol and make and contain the alcohol amount and reach 60%, left standstill 24 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 80%, left standstill 24 hours, and in solution, by volume added 0.8% active carbon, stirred 1.5 hours, filter, filtrate recycling ethanol is concentrated into 200 milliliters, add 4 times of waters for injection, left standstill 12 hours, filter, filtrate is concentrated into 300 milliliters, add ethanol and make and contain alcohol amount and reach 75%, left standstill 26 hours, filter, filtrate is concentrated into 300 milliliters, gets Radix Salviae Miltiorrhizae extract;

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 100 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 2 gram active carbon heated and stirred absorption 40 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700 milliliter, sodium hydroxide NaOH with 8% transfers pH value to 6.5, add 0.5 milliliter of antioxidant sodium sulfite, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 15: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 90 gram Radix Ginseng Rubra 90 grams

Radix Ophiopogonis 300, gram Fructus Schisandrae Chinensis 130 restrained

(p) Radix Ginseng Rubra, Radix Ophiopogonis two, the flavor pulverizing medicinal materials became coarse powder, with the extraction 3 times of confluxing of 75% ethanol of 4 times of amounts, each 1 hour, merge extractive liquid,, cold preservation 12 hours filters, and filtrate recycling ethanol is to there not being the alcohol flavor, cold preservation 12 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 0.9 and restrain medical material, centrifugal, extracting centrifugal liquid is by macroporous resin adsorption, and the water elution with 4 times macroporous resin column volumes discards water lotion, with 2 times of amount column volume amount 20% ethanol elutions, discard eluent, with 15 times of amount column volume 50% ethanol elutions, collect eluent again, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract; The model of the macroporous resin of present embodiment is AB-8 or HPD-100 or NK-9;

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 150 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 20 gram active carbon heated and stirred absorption 20 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700 milliliter, sodium hydroxide NaOH with 8% transfers pH value to 6.5, add 0.5 milliliter of antioxidant sodium sulfite, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 16: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 120 gram Radix Ginseng Rubra 120 grams

Radix Ophiopogonis 375, gram Fructus Schisandrae Chinensis 30 restrained

(p) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials became coarse powder, with the extraction 2 times of confluxing of 45% ethanol of 8 times of amounts, each 2 hours, merge extractive liquid,, cold preservation 48 hours filters, filtrate recycling ethanol is not to there being the alcohol flavor, and cold preservation 48 hours filters, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and the Radix Ophiopogonis extract water-saturated n-butanol with equimultiple is extracted 6 times, or with 3 times of water-saturated n-butanol counter-current extractions, merge n-butyl alcohol, the reclaim under reduced pressure n-butyl alcohol, the residue vacuum drying gets Radix Ginseng Rubra and Radix Ophiopogonis extract;

(q) Fructus Schisandrae Chinensis is collected 180 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(r) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 4 times, each 30 minutes, each amount of water was 4 times of medicinal residues, collecting decoction, filter, relative density 1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 220 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 40 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(s) Radix Salviae Miltiorrhizae decocts with water 4 times, and each amount of water is 4 times, decocting time 1 hour, collecting decoction filters, and filtrate is concentrated into 850 milliliters, add ethanol and make and contain the alcohol amount and reach 80%, left standstill 48 hours, filter, filtrate recycling ethanol is concentrated into 400 milliliters, adds ethanol again and makes and contain alcohol amount and reach 90%, left standstill 48 hours, and in solution, by volume added 1.2% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 330 milliliters, add 5 times of waters for injection, left standstill 20 hours, filter, filtrate is concentrated into 380 milliliters, add ethanol and make and contain alcohol amount and reach 85%, be statically placed in 24 hours, filter, filtrate is concentrated into 380 milliliters, gets Radix Salviae Miltiorrhizae extract;

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 150 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 20 gram active carbon heated and stirred absorption 20 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 900 milliliter, sodium hydroxide NaOH with 12% transfers pH value to 8.0, add 2.0 milliliters of antioxidant sodium pyrosulfite, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1040 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 17: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(u) red ginseng powder is broken into particulate, with alcohol reflux 5 times, each 2 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds water for injection to 400 milliliter, stirs evenly, cold preservation filters, Radix Ginseng Rubra extract;

(v) decoct with water Radix Ophiopogonis 3 times, each 45 minutes, each amount of water was 6 times of medicinal residues, collecting decoction, filter, relative density 1.1 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and also be concentrated into the thick paste shape, add water for injection to 200 milliliter, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 30 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(w) Fructus Schisandrae Chinensis is collected 150 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(x) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 3 times, each 40 minutes, each amount of water was 6 times of medicinal residues, collecting decoction, filter, relative density 1.1 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 200 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 30 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(y) Radix Salviae Miltiorrhizae decocts with water 3 times, and each amount of water is 6 times, decocting time 1.5 hours, collecting decoction filters, and filtrate is concentrated into 700 milliliters, add ethanol and make and contain the alcohol amount and reach 75%, left standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 340 milliliters, adds ethanol again and makes and contain alcohol amount and reach 85%, left standstill 40 hours, and in solution, by volume added 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 280 milliliters, add 4 times of waters for injection, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, add ethanol and make and contain alcohol amount and reach 80%, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, gets Radix Salviae Miltiorrhizae extract;

(z) get Radix Ginseng Rubra extract, add active carbon heated and stirred absorption 30 minutes by 1.5% of filtrate weight, cold slightly, filter, add Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 800 milliliter, the sodium hydroxide NaOH with 10% transfers pH value to 7.3,1.2 milliliters of sodium sulfitees of adding antioxidant, dissolving, filtration, ultrafiltrate is collected in the classification ultrafiltration, adds Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1000 milliliter, embedding is sterilized, and promptly gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 18: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 90 gram Radix Ginseng Rubra 90 grams

Radix Ophiopogonis 300, gram Fructus Schisandrae Chinensis 130 restrained

(u) red ginseng powder is broken into particulate, with alcohol reflux 4 times, each 2.5 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds injection water to 360 milliliter,, stir evenly, cold preservation filters, Radix Ginseng Rubra extract;

(v) decoct with water Radix Ophiopogonis 2 times, each 50 minutes, each amount of water was 8 times of medicinal residues, collecting decoction, filter, relative density 1.05 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and also is concentrated into the thick paste shape, add injection water to 180 milliliter,, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 20 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(w) Fructus Schisandrae Chinensis is collected 120 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(x) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 2 times, each 50 minutes, each amount of water was 8 times of medicinal residues, collecting decoction, filter, relative density 1.05 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 180 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 20 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(y) Radix Salviae Miltiorrhizae decocts with water 2 times, and each amount of water is 8 times, decocting time 2 hours, collecting decoction filters, and filtrate is concentrated into 600 milliliters, add ethanol and make and contain the alcohol amount and reach 60%, left standstill 24 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 80%, left standstill 24 hours, and in solution, by volume added 0.8% active carbon, stirred 1.5 hours, filter, filtrate recycling ethanol is concentrated into 200 milliliters, add 4 times of waters for injection, left standstill 12 hours, filter, filtrate is concentrated into 300 milliliters, add ethanol and make and contain alcohol amount and reach 75%, left standstill 26 hours, filter, filtrate is concentrated into 300 milliliters, gets Radix Salviae Miltiorrhizae extract;

(z) get Radix Ginseng Rubra extract, add active carbon heated and stirred absorption 40 minutes by 0.5% of filtrate weight, cold slightly, filter, add Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700 milliliter, the sodium hydroxide NaOH with 8% transfers pH value to 6.5,0.5 milliliter of adding antioxidant sodium sulfite, dissolving, filtration, ultrafiltrate is collected in the classification ultrafiltration, adds Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960 milliliter, embedding is sterilized, and promptly gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 19: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 120 gram Radix Ginseng Rubra 120 grams

Radix Ophiopogonis 375, gram Fructus Schisandrae Chinensis 30 restrained

(u) red ginseng powder is broken into particulate, with alcohol reflux 5 times, each 1.5 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds injection water to 440 milliliter,, stir evenly, cold preservation filters, Radix Ginseng Rubra extract;

(v) decoct with water Radix Ophiopogonis 4 times, each 30 minutes, each amount of water was 4 times of medicinal residues, collecting decoction, filter, relative density 1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and also is concentrated into the thick paste shape, add injection water to 220 milliliter,, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 40 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(w) Fructus Schisandrae Chinensis is collected 180 milliliters of distillates with steam distillation, and cold preservation gets Fructus Schisandrae Chinensis distillation extraction thing;

(x) getting medicinal residues after the Fructus Schisandrae Chinensis distillation decocts with water 4 times, each 30 minutes, each amount of water was 4 times of medicinal residues, collecting decoction, filter, relative density 1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time and contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and is concentrated into the thick paste shape, add water for injection to 220 milliliter, stir evenly, cold preservation filters, filtrate adds activated carbon and boiled 40 minutes, cold slightly, be filtered to clear and bright, Fructus Schisandrae Chinensis extrat;

(y) Radix Salviae Miltiorrhizae decocts with water 4 times, and each amount of water is 4 times, decocting time 1 hour, collecting decoction filters, and filtrate is concentrated into 850 milliliters, add ethanol and make and contain the alcohol amount and reach 80%, left standstill 48 hours, filter, filtrate recycling ethanol is concentrated into 400 milliliters, adds ethanol again and makes and contain alcohol amount and reach 90%, left standstill 48 hours, and in solution, by volume added 1.2% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 330 milliliters, add 5 times of waters for injection, left standstill 20 hours, filter, filtrate is concentrated into 380 milliliters, add ethanol and make and contain alcohol amount and reach 85%, be statically placed in 24 hours, filter, filtrate is concentrated into 380 milliliters, gets Radix Salviae Miltiorrhizae extract;

(z) get Radix Ginseng Rubra extract, add active carbon heated and stirred absorption 20 minutes by 2.0% of filtrate weight, cold slightly, filter, add Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 900 milliliter, the sodium hydroxide NaOH with 12% transfers pH value to 8.0,2.0 milliliters of adding antioxidant sodium pyrosulfite, dissolving, filtration, ultrafiltrate is collected in the classification ultrafiltration, adds Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1040 milliliter, embedding is sterilized, and promptly gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 20: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 grams

Radix Ophiopogonis 312, gram Fructus Schisandrae Chinensis 156 restrained

(α) red ginseng powder is broken into particulate, with alcohol reflux 5 times, each 2 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds water for injection to 400 milliliter, stirs evenly, cold preservation filters, Radix Ginseng Rubra extract;

(β) decoct with water 3 Radix Ophiopogonis, each 45 minutes, each amount of water was 6 times of medicinal residues, collecting decoction, filter, relative density 1.1 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and also be concentrated into the thick paste shape, add water for injection to 200 milliliter, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 30 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(γ) Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae decoct with water 3 times, and each amount of water is 6 times, decocting time 1.5 hours, collecting decoction filters, and filtrate is concentrated into 700 milliliters, add ethanol and make and contain the alcohol amount and reach 75%, left standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 340 milliliters, adds ethanol again and makes and contain alcohol amount and reach 85%, left standstill 40 hours, and in solution, by volume added 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 280 milliliters, add 4 times of waters for injection, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, add ethanol and make and contain alcohol amount and reach 80%, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(δ) merge Radix Ginseng Rubra extract, Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract, add injection water to 900 milliliter, add 10 gram active carbon heated and stirred absorption 30 minutes, cold slightly, filter, add Radix Ophiopogonis extract and water for injection to 1000 milliliter, with 40% sodium hydroxide NaOH accent pH value to 7.5, add 1.2 milliliters of sodium sulfitees, add 120 milliliters in caffolding agent mannitol, dissolving, filter the classification ultrafiltration, retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate, fill for the second time≤30,000, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 21: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 90 gram Radix Ginseng Rubra 90 grams

Radix Ophiopogonis 300, gram Fructus Schisandrae Chinensis 130 restrained

(α) red ginseng powder is broken into particulate, with alcohol reflux 4 times, each 2.5 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds injection water to 360 milliliter,, stir evenly, cold preservation filters, Radix Ginseng Rubra extract;

(β) decoct with water 2 Radix Ophiopogonis, each 50 minutes, each amount of water was 8 times of medicinal residues, collecting decoction, filter, relative density 1.05 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and also is concentrated into the thick paste shape, add injection water to 180 milliliter,, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 20 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(γ) Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae decoct with water 2 times, and each amount of water is 8 times, decocting time 2 hours, collecting decoction filters, and filtrate is concentrated into 600 milliliters, add ethanol and make and contain the alcohol amount and reach 60%, left standstill 12 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 80%, left standstill 28 hours, and in solution, by volume added 0.8% active carbon, stirred 1.5 hours, filter, filtrate recycling ethanol is concentrated into 200 milliliters, add 4 times of waters for injection, left standstill 12 hours, filter, filtrate is concentrated into 300 milliliters, add ethanol and make and contain alcohol amount and reach 75%, left standstill 26 hours, filter, filtrate is concentrated into 300 milliliters, gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

(δ) merge Radix Ginseng Rubra extract, Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract, add injection water to 800 milliliter, add 2 gram active carbon heated and stirred absorption 40 minutes, cold slightly, filter, add Radix Ophiopogonis extract and water for injection to 1000 milliliter, transfer pH value to 8.0 with 40% sodium hydroxide NaOH, add 0.5 milliliter of sodium sulfite, add 50 milliliters of caffolding agent lactose, dissolving, filtration, the classification ultrafiltration is collected ultrafiltrate, fill, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 22: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

Radix Salviae Miltiorrhizae 120 gram Radix Ginseng Rubra 120 gram grams Radix Ophiopogonis 375

(Q) red ginseng powder is broken into particulate, with alcohol reflux 5 times, each 1.5 hours, merge extractive liquid,, cold preservation filters, filtrate is concentrated into the thick paste shape, adds injection water to 440 milliliter,, stir evenly, cold preservation filters, Radix Ginseng Rubra extract;

(β) decoct with water 4 Radix Ophiopogonis, each 30 minutes, each amount of water was 4 times of medicinal residues, collecting decoction, filter, relative density 1.15 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter, merging filtrate reclaims ethanol and also is concentrated into the thick paste shape, add injection water to 220 milliliter,, stir evenly, cold preservation filters, and filtrate adds activated carbon and boiled 40 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(γ) Radix Salviae Miltiorrhizae decocts with water 4 times, and each amount of water is 4 times, decocting time 1 hour, collecting decoction filters, and filtrate is concentrated into 850 milliliters, add ethanol and make and contain the alcohol amount and reach 80%, left standstill 48 hours, filter, filtrate recycling ethanol is concentrated into 400 milliliters, adds ethanol again and makes and contain alcohol amount and reach 90%, left standstill 48 hours, and in solution, by volume added 1.2% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 330 milliliters, add 5 times of waters for injection, left standstill 20 hours, filter, filtrate is concentrated into 380 milliliters, add ethanol and make and contain alcohol amount and reach 85%, be statically placed in 24 hours, filter, filtrate is concentrated into 380 milliliters, gets Radix Salviae Miltiorrhizae extract;

(δ) merge Radix Ginseng Rubra extract, Radix Salviae Miltiorrhizae extract, add injection water to 1000 milliliter, add 20 gram active carbon heated and stirred absorption 20 minutes, cold slightly, filter, add Radix Ophiopogonis extract and water for injection to 1000 milliliter, transfer pH value to 7.0 with 40% sodium hydroxide NaOH, add 2.0 milliliters of sodium sulfitees, add 200 milliliters of caffolding agent hydroxy propyl-Beta cyclodextrin, dissolving, filtration, the classification ultrafiltration is collected ultrafiltrate, fill, lyophilization promptly gets the extract injection lyophilized powder of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 23: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are:

Radix Salviae Miltiorrhizae 100 gram Radix Ginseng Rubra 100 gram grams Radix Ophiopogonis 312

(a) Radix Salviae Miltiorrhizae decocts with water 3 times, adds 8 times of water gagings for the first time, decocts 2 hours, and second and third time respectively adds 6 times, 5 times water gagings, the each decoction 1.5 hours, collecting decoction filters, filtrate is concentrated into 750 milliliters, adds ethanol and makes and contain the alcohol amount and reach 75%, leaves standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 330 milliliters, add ethanol again and make and contain alcohol amount and reach 85%, left standstill 40 hours, filter, filtrate recycling ethanol, drying under reduced pressure gets Radix Salviae Miltiorrhizae extract;

Embodiment 24: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 150 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 10 gram active carbon heated and stirred absorption 30 minutes, cold slightly, filter, add Radix Salviae Miltiorrhizae extract, add injection water to 800 milliliter, sodium hydroxide NaOH with 10% transfers pH value to 7.3, add 1.2 milliliters of sodium sulfitees of antioxidant, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1000 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 25: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of combined plant medicament extract raw material is:

(p) Radix Ginseng Rubra, Radix Ophiopogonis two flavor pulverizing medicinal materials become coarse powder, conflux with 60% ethanol of 6 times of amounts and to extract 3 times, each 1.5 hours, merge extractive liquid,, cold preservation 40 hours filters, and filtrate recycling ethanol is to there not being the alcohol flavor, cold preservation 40 hours, filter, Radix Ginseng Rubra and Radix Ophiopogonis extract, Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to the 1g medical material, centrifugal, extracting centrifugal liquid is the macroporous resin adsorption of D-101 by model, and the water elution with 8 times of macroporous resin column volumes discards water lotion, with 3 times of amount column volume 30% ethanol elutions, discard eluent again.With 20 times of amount column volumes 40%, collect eluent, reclaim ethanol, be concentrated into 200 milliliters, get Radix Ginseng Rubra and Radix Ophiopogonis extract;

(s) Radix Salviae Miltiorrhizae decocts with water 3 times, and each amount of water is 6 times, decocting time 1.5 hours, collecting decoction filters, and filtrate is concentrated into 700 milliliters, add ethanol and make and contain the alcohol amount and reach 75%, left standstill 40 hours, filter, filtrate recycling ethanol is concentrated into 340 milliliters, adds ethanol again and makes and contain alcohol amount and reach 85%, left standstill 40 hours, and in solution, by volume added 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 280 milliliters, add 4 times of waters for injection, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, add ethanol and make and contain alcohol amount and reach 85%, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, gets Radix Salviae Miltiorrhizae extract;

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add 100 milliliters of waters for injection, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 10 gram active carbon heated and stirred absorption 30 minutes, cold slightly, filter, add Radix Salviae Miltiorrhizae extract, add injection water to 800 milliliter, sodium hydroxide NaOH with 10% transfers pH value to 7.3, add 1.2 milliliters of sodium sulfitees of antioxidant, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 1000 milliliter, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

Embodiment 26: the extract and the extracting method of the combined plant medicine of the treatment coronary atherosclerotic heart disease of present embodiment are: the formulation by weight of plant drug material is:

(h) red ginseng powder is broken into particulate, with alcohol reflux 5 times, each 1.5 hours, merge extractive liquid,, cold preservation filters, filtrate is dense to the thick paste shape, adds water for injection to 400 milliliter, stirs evenly, cold preservation filters, Radix Ginseng Rubra extract;

(i) Radix Salviae Miltiorrhizae decocts with water 4 times, adds 4 times of water gagings at every turn, decocts 1 hour, collecting decoction filters, and filtrate is concentrated into 650 milliliters, add ethanol and make and contain the alcohol amount and reach 70%, left standstill 24 hours, filter, filtrate recycling ethanol is concentrated into 250 milliliters, adds ethanol again and makes and contain alcohol amount and reach 85%, left standstill 24 hours, and in solution, by volume added 1% active carbon, stirred 1 hour, filter, filtrate recycling ethanol is concentrated into 300 milliliters, add 4 times of waters for injection, left standstill 15 hours, filter, filtrate is concentrated into 330 milliliters, add ethanol and make and contain alcohol amount and reach 80%, left standstill 24 hours, filter, filtrate is concentrated into 330 milliliters, gets Radix Salviae Miltiorrhizae extract;

(i) decoct with water 4 Radix Ophiopogonis, each 30 minutes, 4 times of each amount of water, collecting decoction, filter, relative density 1.1 when surveying that filtrate is concentrated into 50 ℃ of heat adds ethanol and carries out ethanol precipitation twice, makes for the first time to contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and also be concentrated into the thick paste shape, add water for injection to 200 milliliter, stir evenly, cold preservation filters, and filtrate adds an amount of activated carbon and boiled 30 minutes, cold slightly, be filtered to clear and bright, Radix Ophiopogonis extract;

(k) get Radix Ginseng Rubra extract, Radix Salviae Miltiorrhizae extract, Radix Ophiopogonis extract, stir, filter, add 10 gram active carbon heated and stirred absorption 30 minutes, cold slightly, filter, add injection water to 800 milliliter, transfer pH value to 7.5 with 10%NaOH, add 1 milliliter of antioxidant sodium sulfite, dissolving, filtration, the classification ultrafiltration, retaining molecular weight: dalton for the first time≤100,000, dalton collects ultrafiltrate for the second time≤30,000, add injection water to 1000 milliliter, embedding is sterilized, and gets the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

The extract of the combined plant medicine of the treatment coronary atherosclerotic heart disease among above-mentioned all embodiment except that injection, is all made the described oral formulations of pharmaceutics, comprises tablet, capsule, soft capsule, drop pill, granule, oral liquid.

Experiment drug effect: the injection of getting above-mentioned part embodiment; select the extract injection of the combined plant medicine of embodiment 12, embodiment 13, embodiment 17, embodiment five, embodiment six, embodiment 20, embodiment 24, embodiment 25, embodiment 26 described treatment coronary atherosclerotic heart diseases; be defined as A, B, C, D, E, F, G, H, I injection respectively, survey its protective effect the coronary heart disease rat

Experiment purpose: inquire into the influence of medicine, estimate the curative effect of medication of treatment coronary heart disease to coronary heart disease rat electrocardiogram and blood fat.

Experimental technique: the wistar male rat is divided into following each group, 10 every group at random.Normal control group (normal saline 10m1.kg ^-1, im.), model control group (normal saline 10ml.kg ^-1, im.), SHENMAI ZHUSHEJI group (4ml.kg ^-1, im.), A injection group (4ml.kg ^-1, im.), B injection group (4ml.kg ^-1, im.), C injection group (4ml.kg ^-1, im.), D injection group (4ml.kg ^-1, im.), E injection group (4ml.kg ^-1, im.), F injection group (4ml.kg ^-1, im.), G injection group (4ml.kg ^-1, im.) H injection group (4ml.kg ^-1, im.), H injection group (4ml.kg ^-1, im.), I injection group (4ml.kg ^-1, im.).Normal group feeding normal feedstuff, all the other respectively organize the feeding high lipid food, and each group is all freely drunk water.Each group is feed 11d altogether, and modeling group and administration group give pituitrin (pie) (10Ukg at 4d and 10d ^-1D ^-1) subcutaneous injection.In the 12nd day, the rat electrocardiogram was respectively organized in test, through neck master A blood sampling, made lipid determination.With reference to the Xu Shi standard, carry out electrocardiogram and observe, possess following condition it; The person is judged as the myocardial ischemia positive: 1. ST section level skew 〉=0.1mv downwards or upwards.2. the T wave height is alarmmed, and surpasses with leading 1/2 of R ripple.3. the T wave height is alarmmed the displacement of companion ST section.Negative judge index: the 1. oblique skew of ST section, or horizontal-shift＜0.1mv; 2. the low flat or two-way inversion of T ripple.

Experimental result: A, B, C, D, E, F, G, H, I injection can obviously reduce the positive incidence rate of myocardial ischemia, reduce the model blood fat, thereby the coronary heart disease rat is played significant protective effect.

The table 1 pair Electrocardiographic influence of coronary heart disease rat

Group	Number of animals (only)	Myocardial ischemia
		Myocardial ischemia		Negative	Positive
		Normal control group model matched group injection group A injection group B injection group C injection group D injection group E injection group F injection group G injection group H injection group I injection group	10 10 10 10 10 10 10 10 10 10 10 10	Negative	Positive	10 1 7 7 8 8 7 8 9 7 8 8	0 9 ^△△ 3 ^ 3 ^ 2 ^ 2 ^ 3 ^ 2 ^ 1 ^ 3 ^ 2 ^ 2 ^

^*Compare with model group p＜0.01; ^{△ △}Compare with the normal control group p＜0.01.

Claims

1. extract for the treatment of the combined plant medicine of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Described weight portion with corresponding relation between the parts by volume is: corresponding 1 ml volumes of 1 gram weight; Its extracting method may further comprise the steps:

(a) get Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae and decoct with water 2～4 times, each 4～8 times of water gagings decocted 1～2 hour at every turn, collecting decoction filters, and filtrate is concentrated into 650～850 parts by volume, add ethanol and make and contain the alcohol amount and reach 60～80%, left standstill 24～48 hours, filter, filtrate recycling ethanol is concentrated into 250～400 parts by volume, adds ethanol again and makes and contain alcohol amount and reach 80～90%, left standstill 24～48 hours, and filtered filtrate recycling ethanol, drying under reduced pressure gets Fructus Schisandrae Chinensis and Radix Salviae Miltiorrhizae extract;

2. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that it being to adopt the described prescription of claim 1 and (a) and (b) and (c) step method.

3. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Its extracting method may further comprise the steps:

4. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Its extracting method may further comprise the steps:

(j) Radix Ophiopogonis and Fructus Schisandrae Chinensis decoct with water 2～4 times, and each 30～50 minutes, 4～8 times of each amount of water, collecting decoction, filter, filtrate is concentrated into the thick paste shape, adds ethanol and carries out ethanol precipitation twice, make for the first time and contain the alcohol amount and reach 80%, make for the second time to contain the alcohol amount and reach 85%, filter merging filtrate, reclaim ethanol and concentrate drying, get Radix Ophiopogonis and Fructus Schisandrae Chinensis extrat;

5. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Its extracting method may further comprise the steps:

(t) get Radix Ginseng Rubra and Radix Ophiopogonis extract, add water for injection 100～150 parts by volume, stir, make the dissolving of Radix Ginseng Rubra and Radix Ophiopogonis extract, filtrate adds 2～20 weight portion active carbon heated and stirred absorption 20～40 minutes, cold slightly, filter, add Fructus Schisandrae Chinensis extrat and Radix Salviae Miltiorrhizae extract, add injection water to 700～900 parts by volume, sodium hydroxide NaOH with 8～12% transfers pH value to 6.5～8.0, add antioxidant 0.5～2.0 parts by volume, dissolving, filter the classification ultrafiltration, collect ultrafiltrate, add Fructus Schisandrae Chinensis distillation extraction thing, add injection water to 960～1040 parts by volume, embedding, sterilize, promptly get the extract injection of the combined plant medicine of described treatment coronary atherosclerotic heart disease.

6. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Its extracting method may further comprise the steps:

7. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that the formulation by weight of combined plant medicament extract raw material is:

Its extracting method may further comprise the steps:

8. the extracting method of the extract of a combined plant medicine for the treatment of coronary atherosclerotic heart disease is characterized in that in claim 2 or 3 or 5 described aforementioned techniques schemes,

For (b) and (p) step, in (b), (p) step, change into and Radix Ginseng Rubra and Radix Ophiopogonis extract are concentrated into every milliliter of medicinal liquid are equivalent to 0.8～1.2 gram medical material, centrifugal, extracting centrifugal liquid to be no less than the water elution of 3 times macroporous resin column volume, discards water lotion by macroporous resin adsorption, with 20%～30% ethanol elution, discard eluent,, collect eluent again with 40%～70% ethanol elution, reclaim ethanol, concentrate Radix Ginseng Rubra and Radix Ophiopogonis extract;

For other steps, with identical in claim 2 or 3 or 5 described aforementioned techniques schemes.

9. the extracting method of the extract of combined plant medicine according to claim 8 is characterized in that described macroporous resin column model is a kind of or its combination among D-101, D-201, AB-8, HPD-100, the NK-9.

10. according to the extracting method of the extract of claim 5 or 6 described combined plant medicines, it is characterized in that described antioxidant is sodium sulfite or sodium sulfite or sodium pyrosulfite or its combination.

11., it is characterized in that the retaining molecular weight of described classification ultrafiltration: dalton, dalton for the second time≤30,000 for the first time≤100,000 according to claim 3 or 5 or the extracting method of the extract of 6 or 7 described combined plant medicines.

12. the extracting method according to the extract of claim 3 or 7 described combined plant medicines is characterized in that described caffolding agent is mannitol or lactose or hydroxy propyl-Beta cyclodextrin or its combination.

13. the oral formulations of making according to the extract of claim 2 or the preparation of 4 described extracting method.