CN100355335C - Method for growing seedlings of Bangiales - Google Patents
Method for growing seedlings of Bangiales Download PDFInfo
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- CN100355335C CN100355335C CNB200510044077XA CN200510044077A CN100355335C CN 100355335 C CN100355335 C CN 100355335C CN B200510044077X A CNB200510044077X A CN B200510044077XA CN 200510044077 A CN200510044077 A CN 200510044077A CN 100355335 C CN100355335 C CN 100355335C
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Abstract
The present invention relates to a method for culturing Bangiales seedlings. After a Bangiales growth history is researched, a conchospore sexual propagation mode and a monosporous asexual propagation mode are formed according to the characteristic of the Bangiales that the Bangiales has two propagation modes. A suitable condition is selected to make the Bangiales mainly propagate in the monosporous mode, and the existent diploid filamentous form trichome formed by the sexual propagation is taken out for singly cultivation, and is used as a stored germplasm of the Bangiales. The method has the optimized culture conditions that sea water is used as a culture medium, the culture temperature is from 19 to 21 DEG C, the illumination intensity is from 800 to 1200 lx, and the photoperiod illumination time is from 10 to 16h. The single and immature Bangiales of which the length is from 0.8 to 1.0cm can release about 400 monospores about 10 days, more than 97% of monospores can germinate into the Bangiales, the germination rate is high, and the method is suitable for large-scale commercial cultivation. Compared with the traditional carpospore taking method for culturing seedlings, the monospore propagation method for cultivating and culturing seedlings has the advantages of simple manufacturing process, convenient operation, short time, high efficiency, etc.
Description
Technical field
The present invention relates to red algae class algal cultivation technical field, more specifically say so about the improvement technology of Bangiales cultivation.
Background technology
Bangiales (Bangia fusco-purpurea) belongs to Rhodophyta, Bangiales, Bangiales section, Bangiales genus, is commonly called as red hair algae, ox hair algae, red hair tongue, red delivering vegetables.Its distribution is extensive, and the subtropics that is present in Eastern and Western hemispheres is to the middle and high tide band than cryogenic region.China north and south is coastal all to have Bangiales to grow, and has now become the very high cultivation marine alga of another economic worth after sea-tangle, laver, undaria pinnitafida and asparagus, and large-area cultivation is especially arranged in Fujian.Bangiales fragrance is unique, and matter sweet taste aquatic foods are nutritious, the equal edible of whole frond.Bangiales contains a large amount of highly unsaturated fatty acid EPA (eicosapentaenoic acid), and according to surveying and determination, the Bangiales of 100g dry weight contains about 437mg EPA, is the highest algae bio of reporting at present of EPA content.In addition, the Bangiales product has the step-down of enriching blood, enriching yin dispel fire and control cardiovascular and cerebrovascular disease effect, edible and medical value height.
China Bangiales cultivation industry is mainly at Fujian Province's Putian City, cultivates largerly, and history is also longer.They adopt the plane mode of collecting seedling to grow seedlings in the 1980's, obtain to diffuse every string shell (20 shell) filamentous day 516.2 ten thousand effects of conchospore.But this method is taken a lot of work and is time-consuming, and it is big to account for pool area, and the time of collecting seedling is long, the seedling cost height.The beginning of the nineties they carry out on a small scale (27m
2) three dimension spat collection test, every string day is diffused 2,060,000 of conchospores, test effect is general, and small scale, does not satisfy growing production demand.Before summary, on the basis of two methods, adopted improved extensive (540m again in 1999
2) the indoor stereo collection technique, every string day is diffused 656.54 ten thousand of conchospore amount average out to, but collects seedling limited time system, is advisable around January annual, and needs often to change seedling shell position to satisfy the illumination demand.
Up to now, Bangiales cultivation all is to utilize the carpospore collection technique, and detailed process is: gather ripe Bangiales kind algae earlier, gather carpospore, cultivate two times of filamentouss, adopt conchospore in several operations such as online, the breed of plunging into the commercial sea.This method exists following shortcomings: the needed time of (1) seedling raising process is long, generally wants the time of some months; (2) gather the frond that carpospore must ripe after fertilization; (3) to prepare a large amount of shells as attaching substratum; (4) in seedling raising process, also to regularly clean shell, prevent long other foreign material, very labor intensive.
Summary of the invention
The object of the present invention is to provide a kind of technology simple, easy to operate, the Bangiales seedling-cultivating method that the time is short, efficient is high.
The present invention discovers that Bangiales has two kinds of modess of reproduction: form the sexual propagation mode of conchospore and form monosporous vegetative propagation mode.Because haploid Bangiales is easy to maturation, be not suitable as germplasm, and two times of filamentouss can rest on the filamentous stage under appropriate condition always.So solution of the present invention is to select under the suitable condition said Bangiales mainly to be bred in the single spore mode, and with in the Bangiales single spore reproductive process, two times of filamentouss of Bangiales that simultaneous sexual propagation forms are chosen the quality saving of independent cultivation as Bangiales.
The seedling-cultivating method of Bangiales of the present invention comprises following main incubation:
1, carpospore is cultivated
The colony that the Bangiales male and female that take a morsel mix, be placed in the culture dish, adding the sterilization seawater (filters with absorbent cotton earlier, boil 10min then) to cultivate, a week is changed twice fresh sterilization seawater, cultivates at ambient temperature earlier, 23-25 on daytime ℃ of control temperature, 16-19 ℃ of night, intensity of illumination 60-100lx, light in the photoperiod/dark: 17h/7h.
After cultivating for two weeks, phenomenon appears expanding in algal filament, and the female plant carposporangium is ripe also to discharge carpospore, and staminiferous plant discharges a large amount of sperms.
2, Bangiales single spore and two times of filamentouss are cultivated.
Then above-mentioned cultivation body is transferred in the illumination box and cultivated.Condition of culture is: temperature 16-29 ℃; Intensity of illumination 300-1200lx; Light application time 10-17h in photoperiod.
After cultivating a week, a large amount of single spore discharge and sprout, and some is directly sprouted in the parent algal filament and is little Bangiales.These are long to the long Bangiales algal filament of about 0.4-1.2cm after five weeks by the little Bangiales that single spore forms.
After cultivating for two weeks, dliploid filamentous algal filament forms, and cultivates separately at the filamentous that the microscopically picking is clean, is used for protecting planting.The preservation condition of two times of filamentouss: with the sterilization seawater is medium, wherein contains NaNO
31.0mmol/L, KH
2PO
40.1mmol/L, FeC
6H
5O
7H
2O 0.01mmol/L, Cobastab
10.01mmol/L, Cobastab
120.00005mmol/L, temperature: 19-24 ℃, illumination: 600-800lx, light application time in the photoperiod: 10-14h.
The optimum condition that Bangiales formed and diffused single spore and the little Bangiales of single spore sprouting formation is: temperature 19-21 ℃; Intensity of illumination 800-1200lx; Light application time 10-16h in photoperiod.Under this condition, length can be diffused about 400 single spore for the single prematurity Bangiales of 0.8-1.0cm about 10 days, and the single spore more than 97% can be sprouted and be Bangiales.
3, Bangiales seedling culture.
The Bangiales frond that picking is formed by single spore on a small quantity is placed in the beaker and cultivates, and the cotton rope of white is spread in the bottom of beaker.The length of Bangiales frond is 0.6-1.2cm, and condition of culture is: temperature 19-21 ℃; Intensity of illumination 800-1200lx; Light in photoperiod/dark: 12h/12h.One week was changed a culture fluid (sterilization seawater).After two weeks, see the many red single spore of good attachment on the white cotton rope, the as seen new longer little Bangiales in six week backs.Cotton rope with the Bangiales seedling is put into marine the cultivation again.
The present invention has studied Bangiales and has diffused the various suitable conditions of monosporous, and has inquired into the feasibility of utilizing Bangiales single spore breeding carrying out cultivation seedling-cultivating with probeing into.
One, temperature influence that the Bangiales single spore is diffused.
With the single frond of Bangiales that forms by single spore more than the tweezers picking of sterilization, in illumination box, cultivate.Former Bangiales length is 1.0cm, condition of culture: 16 ℃ of temperature, 18 ℃, 20 ℃, 23 ℃, 26 ℃, 29 ℃; Intensity of illumination 800lx; Light in photoperiod/dark: 12h/12h.After cultivating for two weeks, the microspore number (Fig. 9) that statistics discharges.Suitable temperature is the key factor that the Bangiales single spore forms and discharges., temperature low excessively (16 ℃) or too high (29 ℃) do not discharge 20 ℃ of optimal temperature conditions of breeding for the Bangiales single spore when all having microspore.
Two, Bangiales algal filament length influence that single spore is diffused.With the single frond of Bangiales that forms by single spore more than the tweezers picking of sterilization, in illumination box, cultivate.Condition of culture: 20 ℃ of temperature; Intensity of illumination 1000lx; Light in photoperiod/dark: 12h/12h.After cultivating a week, the statistics microspore discharges number (Figure 10).When Bangiales also can diffuse single spore when only 0.2cm is long, but quantity seldom, about 13.Bangiales is in the 0.8-1.0cm scope, and the single spore quantity of release is a lot, can reach more than 300 at most.
Three, intensity of illumination influence that the Bangiales single spore is diffused.With the single frond of Bangiales that forms by single spore more than the tweezers picking of sterilization, in illumination box, cultivate.Former Bangiales length is 1.0cm, condition of culture: 20 ℃ of temperature; Intensity of illumination; Be respectively 100lx, 300lx, 400lx, 800lx, 1200lx; Light in photoperiod/dark: 12h/12h.After cultivating for two weeks, the statistics microspore discharges number (Figure 11).When intensity of illumination is lower than 100lx, there is not single spore to discharge, even cultivate (as 8 weeks) for a long time, do not diffuse single spore yet.When intensity of illumination is 300lx, have single spore to diffuse, but negligible amounts some, and the time that discharges postpone slightly, generally need two time-of-weeks.When intensity of illumination more than 400lx, cultivating week age, can to diffuse the single spore intensity of illumination not too big to the number affects that single spore diffuses, best intensity of illumination is between 800-1200lx.
Four, utilize the feasibility of Bangiales single spore breeding carrying out cultivation seedling-cultivating.Bangiales discharges a large amount of single spore under suitable condition, and very fast sprouting is little Bangiales, and germination rate is more than 97%.The Bangiales frond that picking is formed by single spore on a small quantity is placed in the beaker and cultivates, and the cotton rope of white is spread in the bottom of beaker.The length of Bangiales frond is 0.6-1.2cm, and condition of culture is: 20 ℃ of temperature; Intensity of illumination 1000lx; Light in photoperiod/dark: 12h/12h.One week was changed a culture fluid (sterilization seawater).After two weeks, can see the many red single spore of good attachment on the white cotton rope, the as seen new longer little Bangiales (Figure 12) in six week backs.
Experimental result shows that Bangiales diffuses the monosporous optimum condition and is: temperature 19-21 ℃; Intensity of illumination 800-1200lx; Light application time 10-16h in photoperiod.The single spore that diffuses can be sprouted more than 97% and be Bangiales, and the germination rate height is suitable for large-scale commercial cultivation.Obviously, to above-mentioned Bangiales single spore condition of culture: temperature, intensity of illumination, light application time etc. in the photoperiod, select combination again, be not difficult to draw the multiple practical Bangiales single spore cultivation operation scheme that is suitable for, this assembled scheme fully in the present invention in accordance with expectation.
Advantage of the present invention is clearly, utilizes single spore breeding carrying out Bangiales cultivation seedling-cultivating to grow seedlings with traditional fruit picking and compares, and it is simple to have technology, easy to operate, and the time is short, the efficient advantages of higher.
Description of drawings
Fig. 1 is that the photo of phenomenon appears expanding in the Bangiales algal filament;
Fig. 2 is female plant carposporangium maturation and the photo that discharges carpospore;
Fig. 3 is the photo that staminiferous plant discharges a large amount of sperms;
Fig. 4 is the photo that a large amount of single spore discharge;
Fig. 5 is the photo that single spore begins to sprout;
Fig. 6 sprouts to be little Bangiales photo in the parent algal filament;
Fig. 7, Fig. 8 are the photos of the thread algal filament of Bangiales dliploid;
Fig. 9 is the graph of a relation that temperature and single spore diffuse quantity;
Figure 10 diffuses quantitative relation figure for Bangiales algal filament length and single spore;
Figure 11 is the graph of a relation that intensity of illumination and single spore diffuse quantity;
Figure 12 is the photo that the Bangiales single spore is cultivated seedling.
Embodiment
The invention will be further described with by way of example more below.
The colony that the Bangiales male and female that take a morsel mix is placed in the culture dish, adds sterilization seawater (filter with absorbent cotton earlier, boil 10min then) and cultivates, and a week is changed twice fresh sterilization seawater.Cultivate 23-25 on daytime ℃ of temperature, 16-19 ℃ of night, intensity of illumination 60-100lx, light in the photoperiod/dark: 17h/7h earlier at ambient temperature.After two weeks, phenomenon (Fig. 1) appears expanding in algal filament, and the female plant carposporangium is ripe also to discharge carpospore (Fig. 2), and staminiferous plant discharges a large amount of sperms (Fig. 3).
Transfer in the illumination box then and cultivate.Condition of culture is: 20 ℃ of temperature; Intensity of illumination 1000lx; Light in photoperiod/dark: 12h/12h.After cultivating a week, a large amount of single spore discharge (Fig. 4), and some has begun to sprout (Fig. 5), and some direct just sprouting in the parent algal filament is little Bangiales (Fig. 6).Cultivate after five weeks, the Bangiales that single spore forms is long long to about 1cm.
Cultivate two week visible thread algal filament (Fig. 7 of dliploid in back, Fig. 8), cultivate separately at the filamentous that the microscopically picking is clean, be used for protecting and plant, because haploid Bangiales becomes dish to be easy to maturation, be not suitable as germplasm, and the dliploid filamentous can rest on the filamentous stage as long as condition of culture is suitable always.Two times of filamentouss are as the condition of quality saving: with the sterilization seawater is medium, wherein contains NaNO
31.0mmol/L, KH
2PO
40.1mmol/L, FeC
6H
5O
7H
2O 0.01mmol/L, Cobastab
10.01mmol/L, Cobastab
120.00005mmol/L, temperature: 19-24 ℃, illumination: 600-800lx, light application time in the photoperiod: 10-14h.
The cultivation of embodiment 2 Bangiales seedling
The Bangiales frond that picking is formed by single spore on a small quantity is placed in the beaker and cultivates, and the cotton rope of white is spread in the bottom of beaker.The length of Bangiales frond is 0.6-1.2cm, and condition of culture is: 20 ℃ of temperature; Intensity of illumination 1000lx; Light in photoperiod/dark: 12h/12h.One week was changed a culture fluid (sterilization seawater).After two weeks, can see the many red single spore of good attachment on the white cotton rope, the as seen new longer little Bangiales in six week backs.Cotton rope with the Bangiales seedling is put into marine the cultivation again.
Claims (3)
1, a kind of seedling-cultivating method of Bangiales is characterized in that said Bangiales mainly breeds in the single spore mode; Two times of filamentous algal filaments that sexual propagation is formed are cultivated separately simultaneously, with the quality saving as Bangiales, comprise following main incubation:
The 1-1 carpospore is cultivated
Get the colony that the Bangiales male and female mix, be placed in the culture dish, add the sterilization seawater and cultivate, a week is changed twice fresh fertilized seawater, and incubated at room temperature is after two weeks, and phenomenon appears expanding in algal filament, and the female plant carposporangium is ripe also to discharge carpospore, and staminiferous plant discharges a large amount of sperms; 23-25 ℃ of control day temperature, 16-19 ℃ of night, intensity of illumination 60-100lx; Light in photoperiod/dark: 17h/7h;
Two times of filamentouss of 1-2 Bangiales single spore and Bangiales are cultivated:
Then above-mentioned culture is transferred in the illumination box and cultivated, and control: cultivation temperature 16-29 ℃, intensity of illumination 300-1200lx; Light application time 10-17h in photoperiod;
After 1-2-1 cultivated a week, single spore discharged and sprouts, and some is directly sprouted in the parent algal filament and is little Bangiales;
After 1-2-2 cultivated for two weeks, two times of filamentouss formed, and cultivate separately at the filamentous that the microscopically picking is clean, as the quality saving of Bangiales;
After 1-2-3 cultivated for five weeks, the little Bangiales that is formed by single spore grew up to the long Bangiales algal filament of 0.4-1.2cm;
1-3 Bangiales seedling culture:
Choose the Bangiales algal filament that is formed by single spore on a small quantity, be placed in the beaker and cultivate, the cotton rope of white is spread in the bottom of beaker, and choosing Bangiales algal filament length is 0.6-1.2cm; Condition of culture is: temperature 19-21 ℃, and intensity of illumination 800-1200lx, light in the photoperiod/dark: 12h/12h, a week is changed once sterilizing seawater culture fluid;
1-3-1 adheres to red single spore after two weeks on the cotton rope of white, after six weeks, newly grow little Bangiales;
1-3-2 is put into marine the cultivation again with the cotton rope of Bangiales seedling.
2,, it is characterized in that mainly the condition of breeding Bangiales in the single spore mode is according to the described seedling-cultivating method of claim 1: cultivation temperature 19-21 ℃, intensity of illumination 800-1200lx, light application time 10-16h in the photoperiod; Choosing Bangiales algal filament length is 0.8-1.0cm.
3, according to the described seedling-cultivating method of claim 1, it is characterized in that the preservation condition of said two times of filamentouss: with the sterilization seawater is medium, wherein contains NaNO3 1.0mmol/L, KH
2PO
40.1mmol/L, FeC
6H
5O
7H
2O 0.01mmol/L, Cobastab
10.01mmol/L, Cobastab
120.00005mmol/L, temperature: 19-24 ℃, illumination: 600-800lx, light application time in the photoperiod: 10-14h.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975840A (en) * | 2014-05-30 | 2014-08-13 | 中国水产科学研究院黄海水产研究所 | Nutrient solution for fresh water bangia |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102986530B (en) * | 2012-11-25 | 2014-01-01 | 溧阳市天目湖保健品有限公司 | Tissue culture method of gloiopeltis |
| CN110839525A (en) * | 2019-10-31 | 2020-02-28 | 江苏省海洋水产研究所 | Preparation method of rhodobryum roseum germplasm filament |
| CN115005088A (en) * | 2022-07-15 | 2022-09-06 | 厦门大学 | Land-based suspension propagation culture method for cabbages |
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Non-Patent Citations (3)
| Title |
|---|
| 关于藻类的养殖技术之一红毛藻养殖中采单孢子技术要点 胡开忠，曾雪锋.中国水产，第9期 2000 * |
| 红毛菜大面积立体采果孢子苗技术 黄春恺.齐鲁渔业，第20卷第4期 2003 * |
| 红毛菜室内苗种培育技术规程 黄春恺.福建水产，第4期 2002 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103975840A (en) * | 2014-05-30 | 2014-08-13 | 中国水产科学研究院黄海水产研究所 | Nutrient solution for fresh water bangia |
| CN103975840B (en) * | 2014-05-30 | 2015-07-01 | 中国水产科学研究院黄海水产研究所 | Nutrient solution for fresh water bangia |
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