CN100345959C - Liquid changing device for artificial skin production and cell culture engineering - Google Patents
Liquid changing device for artificial skin production and cell culture engineering Download PDFInfo
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- CN100345959C CN100345959C CNB2006100540166A CN200610054016A CN100345959C CN 100345959 C CN100345959 C CN 100345959C CN B2006100540166 A CNB2006100540166 A CN B2006100540166A CN 200610054016 A CN200610054016 A CN 200610054016A CN 100345959 C CN100345959 C CN 100345959C
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- peristaltic pump
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Abstract
The present invention relates to a program liquid changing device for artificial skin production and cell culture, which is formed by assembling a conveying device 1, a culture dish 2, a culture medium waste liquid bottle 3, a culture medium new liquid bottle 4, a physiological saline bottle 5, a peristaltic pump 6, a three-way pipe 7, an electrical control box 8, a mechanical hand 9, a lifter 10, an ultraviolet lamp 11, a liquid charging pipe 12 and a suction pipe 13. The present invention has the advantages of simple and reasonable structure, rapid and convenient operation, human power saving, material resource saving, high liquid changing speed, short period, thorough replacement, high work efficiency, no pollution in the liquid changing process, production cost reduction, and simplification of the tissue engineering industrializing process. The present invention which can be used for changing liquid for a large quantity of cell and tissue engineering products in a short time is especially suitable for the large-scale production and the industrialized production process steps of the cell and tissue engineering products.
Description
One, technical field
The invention belongs to what zymetology or microbiology device, particularly laboratory and plant-scale device of biomass cells cultivation and field of tissue engineering technology.
Two, background technology
Organization engineering skin is the emerging technology field that grows up over 30 years, progressively moves towards clinical from the laboratory.It is widely used in tumour, inflammation, wound, burn etc. and causes the damage, structure deteriorate of skin so that afunction in reason.But in the practical application of cell cultures and artificial skin organization of production engineering, for cells and replacement of products substratum are daily and heavy works in a large number, realize industrialization, reach scale operation, making a large amount of cells and artificial skin change liquid can finish automatically, must develop the program liquid changing device that is applicable to artificial skin production and cell cultures.
In the prior art, having a name to be called " method and the device that are used for changing liquid cultivation matrix " application number is 99100414.0 the Chinese invention patent bulletin of obtaining the authorization on October 12nd, 2005.This device comprises that is used for the pipeline that first container transports old substratum and replaces old substratum with new substratum; What link to each other with pipeline is storage tank.One end of pipeline is connected on the vacuum source, and from culture vessel, draw old substratum by the control vacuum unit, meanwhile, extract the new substratum storage tank of packing into out from new substratum source, unclamp vacuum control, transport and shed vacuum thereby stop fluid, the unlatching with control device can make the discharging of substratum storage tank in addition, enters in the cell culture container by pressure.This device has effectively reduced the workload that single changes liquid really, and reduce and pollute probability, but the thinking of this device remains and is confined to rely on manpower to finish single to change liquid, therefore produces cell and tissue engineering product and inapplicable for the industrialization of big workload.
In the prior art, the Chinese invention patent that also has a name to be called " cellular environment liquid changing device " the application number 03141673.x bulletin of obtaining the authorization on September 28th, 2005.This device comprises that two groups of structures independently change liquid pump and drive two groups of pump driving mechanisms that change the liquid pump synchronous interaction, with two two groups of liquid lines that change liquid pump import and export connection, and the driving mechanism for switches of the influidic switch of two groups of liquid line switchings of control and two groups of switch synchronous interactions of driving.This device can carry out the quick displacement of two kinds of cellular environment liquid simultaneously, has mainly solved in the cell electricity fusion experiment process, and cell is being preserved, cultivated and the different requirements of the different steps cell sample that merges to environmental liquids.But the same with preceding a kind of device, this device can not provide the liquid changing device of a kind of mechanize, automatization and sequencing, and liquid is saved time and human and material resources for a large amount of cells and artificial skin change.
At present, the relevant program liquid changing device that is applicable to artificial skin production and cell cultures yet there are no the open source literature report.
Three, summary of the invention
Defective and blank at the prior art existence, the present invention proposes a kind of program liquid changing device that is used for artificial skin production and cell cultures of meter, make and change liquid process automation, mechanize and sequencing, alleviated the labor capacity of changing substratum significantly, make that changing liquid potential pollution probability significantly reduces, make the device of design be particularly useful for not only can reducing production costs but also can simplify the organizational project industrialization process in the scale operation and industrialization production process of cell and tissue engineering product.
The technical solution used in the present invention is as follows.
A kind of program liquid changing device that is used for artificial skin production and cell cultures is characterized in that:
1) it is assembled by transport unit, culture dish, substratum waste liquid bottle, substratum new solution bottle, physiological saline bottle, peristaltic pump, Y-tube, electric control box, mechanical manipulator, elevator, ultraviolet lamp, liquid adding bottle and suction pipe;
2) mechanical manipulator and elevator flexibly connect, and liquid adding bottle and suction pipe are vertically mounted on the mechanical manipulator, make liquid adding bottle and suction pipe horizontally rotate or move up and down with mechanical manipulator;
3) substratum waste liquid bottle, substratum new solution bottle and physiological saline bottle are arranged on the contour table top, the bottleneck coupling has cork stopper and is inserted with Glass tubing, the Glass tubing upper end of substratum waste liquid bottle links to each other with the go side always of Y-tube by the flexible pipe that cock is housed, the Glass tubing upper end of physiological saline bottle links to each other with the go side of turning of Y-tube by the flexible pipe that cock is housed, the straight-through end of another of Y-tube links to each other with peristaltic pump by flexible pipe, the Glass tubing upper end of substratum new solution bottle links to each other with peristaltic pump by the flexible pipe that cock is housed, and the upper end of liquid adding bottle and suction pipe links to each other with peristaltic pump by flexible pipe respectively;
4) on substratum waste liquid bottle, substratum new solution bottle and physiological saline bottle table top next door, be provided with transport unit, culture dish is shelved on the travelling belt of transport unit;
5) electric control loop of transport unit, peristaltic pump, mechanical manipulator, elevator and ultraviolet lamp is in parallel in electric control box, is realized the operation of transport unit, peristaltic pump, mechanical manipulator, elevator and ultraviolet lamp is controlled by electric control box.
The program of apparatus of the present invention is changed the liquid process: at first, culture dish is placed on the travelling belt, travelling belt is sent to culture dish and changes the liquid place below liquid adding bottle and the suction pipe, with mechanical manipulator liquid adding bottle and suction pipe are adjusted to proper height again, make it to add new liquid or draw waste liquid, the substratum waste liquid bottle is drawn and be transported to waste liquid in the substratum with peristaltic pump, then, draw the pipeline that normal saline flushing was carried the substratum waste liquid with peristaltic pump, be delivered to liquid adding bottle with the new liquid of peristaltic pump absorption substratum more at last and be added in the culture dish.After operation is finished, move decline again after mechanical manipulator rises also, liquid adding bottle and suction pipe are immersed in the vial.Before the operation earlier with ultraviolet lamp to the Working environment sterilization and disinfection.
Program liquid changing device of the present invention, simple and reasonable, swift and convenient to operate, can change liquid for a large amount of cell and tissue engineering products at short notice, use manpower and material resources sparingly; It change that liquid speed is fast, the cycle is short, change thoroughly, high efficiency, and it is pollution-free to change the liquid process; Be particularly useful in the scale operation and industrialization production process of cell and tissue engineering product, can reduce production costs and can simplify the organizational project industrialization process.
Four, description of drawings
Fig. 1 is a kind of structural representation that is used for the program liquid changing device of artificial skin production and cell cultures of the present invention;
Fig. 2 is the electric control circuit schematic diagram of program liquid changing device of the present invention.
In the drawings, 1-transport unit, 2-culture dish, 3-substratum waste liquid bottle, 4-substratum new solution bottle, 5-physiological saline bottle, 6-peristaltic pump, 7-Y-tube, 8-electric control box, 9-mechanical manipulator, 10-elevator, 11-ultraviolet lamp, 12-liquid adding bottle, 13-suction pipe.
Five, embodiment
The concrete structure that further specifies program liquid changing device of the present invention below in conjunction with accompanying drawing carries out the primary process that program is changed liquid with it.
A kind of program liquid changing device that is used for artificial skin production and cell cultures is characterized in that:
1) it is assembled by transport unit 1, culture dish 2, substratum waste liquid bottle 3, substratum new solution bottle 4, physiological saline bottle 5, peristaltic pump 6, Y-tube 7, electric control box 8, mechanical manipulator 9, elevator 10, ultraviolet lamp 11, liquid adding bottle 12 and suction pipe 13;
2) mechanical manipulator 9 flexibly connects with elevator 10, and liquid adding bottle 12 and suction pipe 13 are vertically mounted on the mechanical manipulator 9, makes liquid adding bottle 12 and suction pipe 13 horizontally rotate or move up and down with mechanical manipulator 9;
3) the substratum waste liquid bottle 3, substratum new solution bottle 4 and physiological saline bottle 5 are arranged on the contour table top, the bottleneck coupling has cork stopper and is inserted with Glass tubing, the Glass tubing upper end of substratum waste liquid bottle 3 links to each other with the go side always of Y-tube 7 by the flexible pipe that cock is housed, the Glass tubing upper end of physiological saline bottle 5 links to each other with the go side of turning of Y-tube 7 by the flexible pipe that cock is housed, the straight-through end of another of Y-tube 7 links to each other with peristaltic pump 6 by flexible pipe, the Glass tubing upper end of substratum new solution bottle 4 links to each other with peristaltic pump 6 by the flexible pipe that cock is housed, and the upper end of liquid adding bottle 12 and suction pipe 13 links to each other with peristaltic pump 6 by flexible pipe respectively;
4) on substratum waste liquid bottle 3, substratum new solution bottle 4 and physiological saline bottle 5 table tops next door, be provided with transport unit 1, culture dish 2 is shelved on the travelling belt of transport unit 1;
5) electric control loop of transport unit 1, peristaltic pump 6, mechanical manipulator 9, elevator 10 and ultraviolet lamp 11 is in parallel in electric control box 8, is realized the operation of transport unit 1, peristaltic pump 6, mechanical manipulator 9, elevator 10 and ultraviolet lamp 11 is controlled by electric control box 8.
The primary process that program is changed liquid is as follows: sterilized with 11 pairs of Working environment virus killings of ultraviolet lamp earlier before operating; When the cell or tissue engineering product need be changed substratum, at first, culture dish 2 is placed on the travelling belt of transport unit 1, drive mechanical manipulator 9 by elevator 10 liquid adding bottle 12 and suction pipe 13 are placed below the liquid level of culture dish 2; Startup peristaltic pump 6 is drawn the waste liquid in the culture dish 2 by suction pipe 13 and is delivered in the substratum waste liquid bottle 3, again the physiological saline in the physiological saline bottle 5 is drawn with peristaltic pump 6 and removed to wash the pipeline of carrying the substratum waste liquid, be delivered to liquid adding bottle 12 with the fresh culture in the peristaltic pump 6 absorption substratum new solution bottles 4 at last and join in the culture dish 2.The whole liquid process of changing can both be finished automatically by the function key of operation electric control box 8.
Claims (1)
1, a kind of program liquid changing device that is used for artificial skin production and cell cultures is characterized in that:
1) it is assembled by transport unit (1), culture dish (2), substratum waste liquid bottle (3), substratum new solution bottle (4), physiological saline bottle (5), peristaltic pump (6), Y-tube (7), electric control box (8), mechanical manipulator (9), elevator (10), ultraviolet lamp (11), liquid adding bottle (12) and suction pipe (13);
2) mechanical manipulator (9) and elevator (10) flexibly connect, and liquid adding bottle (12) and suction pipe (13) are vertically mounted on the mechanical manipulator (9), make liquid adding bottle (12) and suction pipe (13) horizontally rotate or move up and down with mechanical manipulator (9);
3) substratum waste liquid bottle (3), substratum new solution bottle (4) and physiological saline bottle (5) are arranged on the contour table top, the bottleneck coupling has cork stopper and is inserted with Glass tubing, the Glass tubing upper end of substratum waste liquid bottle (3) links to each other with the go side always of Y-tube (7) by the flexible pipe that cock is housed, the Glass tubing upper end of physiological saline bottle (5) links to each other with the go side of turning of Y-tube (7) by the flexible pipe that cock is housed, the straight-through end of another of Y-tube (7) links to each other with peristaltic pump (6) by flexible pipe, the Glass tubing upper end of substratum new solution bottle (4) links to each other with peristaltic pump (6) by the flexible pipe that cock is housed, and the upper end of liquid adding bottle (12) and suction pipe (13) links to each other with peristaltic pump (6) by flexible pipe respectively;
4) on substratum waste liquid bottle (3), substratum new solution bottle (4) and physiological saline bottle (5) table top next door, be provided with transport unit (1), culture dish (2) is shelved on the travelling belt of transport unit (1);
5) electric control loop of transport unit (1), peristaltic pump (6), mechanical manipulator (9), elevator (10) and ultraviolet lamp (11) is in parallel in electric control box (8), is realized the operation of transport unit (1), peristaltic pump (6), mechanical manipulator (9), elevator (10) and ultraviolet lamp (11) is controlled by electric control box (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100540166A CN100345959C (en) | 2006-01-11 | 2006-01-11 | Liquid changing device for artificial skin production and cell culture engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100540166A CN100345959C (en) | 2006-01-11 | 2006-01-11 | Liquid changing device for artificial skin production and cell culture engineering |
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| Publication Number | Publication Date |
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| CN1840647A CN1840647A (en) | 2006-10-04 |
| CN100345959C true CN100345959C (en) | 2007-10-31 |
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| CNB2006100540166A Expired - Fee Related CN100345959C (en) | 2006-01-11 | 2006-01-11 | Liquid changing device for artificial skin production and cell culture engineering |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481654B (en) * | 2008-12-16 | 2011-04-06 | 中国人民解放军第三军医大学第三附属医院 | Synthetic apparatus for constructing tissue engineering seed cell and scaffold complex |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015078137A1 (en) * | 2013-11-27 | 2015-06-04 | 鲁建国 | Epidermal cell collecting instrument and dynamic collecting method thereof |
| CN103614295B (en) * | 2013-11-27 | 2014-07-16 | 绍兴振德医用敷料有限公司 | Epidermal cell enriching instrument and dynamic enriching method thereof |
| CN105997300B (en) * | 2016-05-12 | 2017-08-25 | 江西高大生物医疗科技有限公司 | A kind of artificial skin automatically generates line |
| CN109735453A (en) * | 2019-02-26 | 2019-05-10 | 陕西师范大学 | A kind of breast cancer cell line MCF-7 at the uniform velocity changes liquid culture apparatus |
| CN112574961A (en) * | 2020-12-28 | 2021-03-30 | 兆丰华生物科技(南京)有限公司 | Preparation method of cell vaccine antigen |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH078260Y2 (en) * | 1989-08-23 | 1995-03-01 | 豊田合成株式会社 | Air conditioning grill |
| CN1234440A (en) * | 1998-01-15 | 1999-11-10 | 创新装置有限公司 | Method and apparatus for changing liquid cultivation matrix |
| CN1482233A (en) * | 2003-07-17 | 2004-03-17 | 中国科学院上海技术物理研究所 | Cellular environment liquid changing device |
-
2006
- 2006-01-11 CN CNB2006100540166A patent/CN100345959C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH078260Y2 (en) * | 1989-08-23 | 1995-03-01 | 豊田合成株式会社 | Air conditioning grill |
| CN1234440A (en) * | 1998-01-15 | 1999-11-10 | 创新装置有限公司 | Method and apparatus for changing liquid cultivation matrix |
| CN1482233A (en) * | 2003-07-17 | 2004-03-17 | 中国科学院上海技术物理研究所 | Cellular environment liquid changing device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481654B (en) * | 2008-12-16 | 2011-04-06 | 中国人民解放军第三军医大学第三附属医院 | Synthetic apparatus for constructing tissue engineering seed cell and scaffold complex |
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| CN1840647A (en) | 2006-10-04 |
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Granted publication date: 20071031 Termination date: 20120111 |