CN100345559C - Medicine from black snake for treating vitiligo - Google Patents
Medicine from black snake for treating vitiligo Download PDFInfo
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- CN100345559C CN100345559C CNB2005100416319A CN200510041631A CN100345559C CN 100345559 C CN100345559 C CN 100345559C CN B2005100416319 A CNB2005100416319 A CN B2005100416319A CN 200510041631 A CN200510041631 A CN 200510041631A CN 100345559 C CN100345559 C CN 100345559C
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- chinese medicine
- zaocys
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- leukoderma
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Abstract
The present invention relates to a Chinese medicine paste for treating dermatosis, more specifically to a Chinese medicine for treating leukoderma, namely garter snake for treating leukoderma and a preparing method thereof. The Chinese medicine paste is prepared from Chinese medicines of garter snake, cantharis, dahurian angelica root, lithospermi, flavescent sophora root, cnidium fruit and malaytea scurfpea fruit. The Chinese medicines are weighed according to the weight requirement and are decocted, filtered and concentrated; the Chinese medicines are uniformly mixed with vaseline and lanolin molten at the temperature of 60 after being concentrated; after chlorhexidine acetate and borneo camphor which is dissolved by ethanol are added to the Chinese medicines, and the chlorhexidine acetate, the borneo camphor and the Chinese medicines are uniformly mixed, a finished product is obtained. The active ingredients of the Chinese medicine for treating leukoderma comprise biotoxin, such as garter snake protein, snake muscle aldolase, cantharidin, angelicotoxin, etc., a manganese containing compound and a plurality of enzymes. After the Chinese medicine paste is applied to parts with leukoderma, the Chinese medicine paste which is activated by ultraviolet rays or solar radiation can remove leukoderma and restore pigments; excellent therapeutic effect is obtained.
Description
(1) technical field
The present invention relates to the dermopathic Chinese medicine unguentum of a kind of treatment, a kind of specifically Chinese medicine for the treatment of leucoderma disease is Zaocys leucoderma gram and production method thereof.
(2) technical background
Vitiligo is a kind of posteriority depigmentation mucocutaneous disease, these type of sick difficult miscellaneous diseases that belong to, though this is sick superficial, direct harmful to human is not healthy, influences people's image appearance, often causes serious mental maladjustment to the patient.At present this sick Therapeutic Method there are many kinds, general all according to the different state of an illness, by stages, typing takes photochemical therapy, glucocorticoid, from body surface skin grafting dermepenthesis treatment etc., these therapies are not only serious to human body infringement, but also can not life-time service, and effect is not very good again.
(3) summary of the invention city
Show through experimentation: the melanocyte of patients with vitiligo skin (MC) is all in damaged condition, and growth is obviously quickened after adding exogenous catalase.Further experiment shows; This phenomenon is vitiligo epidermis hydrogen peroxide (H
2O
2) increase and the result that can not normally degrade.And the gathering of excessive hydrogen peroxide has " drifting " effect to skin.Being a large amount of melanocytes (MC) disappearances, is vitiligo and form white macula.The object of the present invention is to provide a kind of hydrogen peroxide degradation that can make the vitiligo epidermis, add exogenous catalase, and with after ultraviolet or the sun exposure activation, hydrogen peroxide (H
2O
2) just can be degraded into water and oxygen rapidly, thus melanocyte (MC) is increased, to reach the leukodermic purpose of treatment.Medicine with the treatment leucoderma disease of Zaocys preparation is made by Chinese medicine Zaocys 70g, Mylabris 45g, Radix Angelicae Dahuricae 90g, Radix Arnebiae (Radix Lithospermi) 70g, Radix Sophorae Flavescentis 70g, Fructus Cnidii 70g, Fructus Psoraleae 90g.Decoct after Zaocys, Mylabris, the Radix Angelicae Dahuricae, Radix Arnebiae (Radix Lithospermi), Radix Sophorae Flavescentis, Fructus Cnidii, Fructus Psoraleae taken by weighing by above-mentioned weight requirement, filter, concentrate, concentrate back and 60 ℃ of fused vaseline, lanoline mix homogeneously, adding is mix homogeneously behind the Borneolum Syntheticum of dissolve with ethanol and chlorhexidine acetate, gets product.Its active component of the medicine that the present invention produced has plurality of enzymes such as biotoxins such as Zaocys toxalbumin, cantharidin, angelicotoxin and snake musle a1do1ase.After these product are applied in the leucoderma disease affected part, activate, can remove white macula, recover pigment, obtained beyond thought therapeutic effect through ultraviolet or sun exposure.
The name of the present invention in disease prevention and control center, Shaanxi Province survey report is called Zaocys Bai Dianke
| Name of product | Zaocys Bai Dianke | Connect the sample date | On 06 05th, 2002 |
| Inspection item | Bacteria quantified is killed test | Inspected the date | On 06 17th, 2002 |
One, equipment:
1, test strain: staphylococcus aureus (ATCC6538) is provided 5-12 generation by Military Medical Science Institute.
2, disinfectant: censorship Zaocys Bai Dianke.
3, nertralizer: the PBS solution that contains 3.0% tween 80+1.0% lecithin+0.5% sodium thiosulfate.
4,0.03mol/LPBS contains the 0.03mol/LPBS liquid of 1% peptone, culture medium etc.
5, aseptic measuring pipette, aseptic plate, test tube, constant temperature culture etc.
Two, method:
1, carries out according to " disinfection technology standard " third edition first fascicle.
2, the nertralizer qualification test adopts the suspension method qualification test, and decontaminant concentration is a stock solution, and to staphylococcus aureus (ATCC6538) effect 5min, test temperature is 20 ± 2 ℃, the test triplicate.
3, quantitative disinfecting test is the suspension method test.Test temperature is 20 ± 2 ℃, the test triplicate.
Three, result of the test:
1, nertralizer qualification test
1.1 the test triplicate, the average error rate between the 3rd, 4,5 group three groups is 8.95%.
1.2 the nertralizer qualification test the results are shown in Table 1.
Table 1 nertralizer qualification test result
| Group | Each test growth clump count (cfu/ sheet) | Average growth clump count (cfu/ sheet) | ||
| 1 | 2 | 3 | ||
| 1 2 3 4 5 6 7 | 0 1.20×10 2 1.74×10 6 1.52×10 6 1.96×10 6 0 0 | 0 7.00×10 1 1.68×10 6 1.40×10 6 1.88×10 6 0 0 | 0 1.40×10 2 1.86×10 6 1.66×10 6 2.12×10 6 0 0 | 0 2.26×10 2 1.76×10 6 1.52×10 6 1.98×10 6 0 0 |
| 8 | 0 | 0 | 0 | 0 |
2, to the killing effect of test organisms
Bactericidal assay result: under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 10min, 50% (v/v) diluent effect 15min reach more than 99.90% the average kill ratio of staphylococcus aureus (ATCC6538).The results are shown in Table 1.
Table 1 Zaocys Bai Dianke is to the killing effect of staphylococcus aureus
| Concentration | Average kill ratio and the scope (%) thereof of different action times (min) | |||
| 5 | 10 | 15 | 20 | |
| Stock solution 50% (v/v) | 99.83 (99.82-99.86) 99.79 (99.75-99.83) | 99.92 (99.91-99.94) 99.87 (99.84-99.91) | 99.94 (99.93-99.96) 99.92 (99.90-99.94) | 99.97 (99.96-99.99) 99.95 (99.92-99.97) |
Annotate: the average clump count of positive controls is 2.24 * 10
6Cfu/ml (scope: 1.98 * 10
6-2.46 * 10
6Cfu/ml) negative control group asepsis growth
Four conclusions:
1, the PBS solution that contains 3.0% tween 80+2.0% lecithin+0.5% sodium thiosulfate can neutralize fully to Zaocys Bai Dianke stock solution, and this nertralizer and neutralized reaction product do not have influence to staphylococcus aureus (ATCC6538).
2, under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 30min reaches more than 99.90% the average kill ratio of staphylococcus aureus (ATCC6538).
| Name of product | Zaocys Bai Dianke | Connect the sample date | On 06 05th, 2002 |
| Inspection item | Bacteria quantified is killed test | Inspected the date | On 06 17th, 2002 |
One, equipment:
1, test strain: escherichia coli (8099) are provided 5-12 generation by Military Medical Science Institute.
2, disinfectant: censorship Zaocys Bai Dianke.
3, nertralizer: the PBS solution that contains 3.0% tween 80+1.0% lecithin+0.5% sodium thiosulfate.
4,0.03mol/LPBS contains the 0.03mol/LPBS liquid of 1% peptone, culture medium etc.
5, aseptic measuring pipette, aseptic plate, test tube, constant incubator etc.
Two, method:
1, carries out according to " disinfection technology standard " third edition first fascicle.
2, quantitative disinfecting test is the suspension method test.Test temperature is 20 ± 2 ℃, the test triplicate.
Three result of the tests:
Bactericidal assay result: under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 15min, 50% (v/v) diluent effect 20min reach more than 99.90% the average kill ratios of escherichia coli (8099).The results are shown in Table 1.
Table 1 Zaocys Bai Dianke is to colibacillary killing effect
| Concentration | Average kill ratio and the scope (%) thereof of different action times (min) | |||
| 5 | 10 | 15 | 20 | |
| Stock solution | 99.78 (99.74-99.82) | 99.86 (99.85-99.88) | 99.92 (99.91-99.93) | 99.94 (99.93-99.96) |
| 50％(v/v) | 99.74 | 99.81 | 99.87 | 99.92 |
| (99.71-99.78) | (99.79-99.84) | (99.84-99.89) | (99.90-99.94) | |
Annotate: the average clump count 2.24 * 10 of positive controls
6Cfu/ml (scope: 1.98 * 10
6-2.46 * 10
6Cfu/ml) negative control group asepsis growth.
Four, conclusion:
Under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 15min, 50% (v/v) diluent effect 20min reach more than 99.90% the average kill ratios of escherichia coli (8099).
| Name of product | Zaocys Bai Dianke | Connect the sample date | On 06 05th, 2002 |
| Inspection item | Bacteria quantified is killed test | Inspected the date | On 06 17th, 2002 |
One, equipment:
1, test strain: Candida albicans (ATCC10231) is provided 6-11 generation by Military Medical Science Institute.
2, disinfectant: censorship Zaocys Bai Dianke.
3, nertralizer: the PBS solution that contains 3.0% tween 80+1.0% lecithin+0.5% sodium thiosulfate.
4,0.03mol/LPBS contains the 0.03mol/LPBS liquid of 1% peptone, culture medium etc.
5, aseptic measuring pipette, aseptic plate, test tube, constant incubator etc.
Two, method:
1, carries out according to " disinfection technology standard " third edition first fascicle.
2, the nertralizer qualification test adopts the suspension method test, and decontaminant concentration is a stock solution, and to Candida albicans (ATCC10231) effect 5min, test temperature is 20 ± 2 ℃, the test triplicate.
3, quantitative disinfecting test is the suspension method test.Test temperature is 20 ± 2 ℃, the test triplicate.
Three, result of the test:
1, nertralizer qualification test
1.1 test triplicate, the average error rate between the 3rd, 4,5 group are 8.95%.
1.2 the nertralizer qualification test the results are shown in Table 1.
Table 1 nertralizer qualification test result
| Group | Each test growth clump count (cfu/ sheet) | Average growth clump count (cfu/ sheet) | ||
| 1 | 2 | 3 | ||
| 1 2 3 4 5 6 7 8 | 0 1.20×10 2 1.74×10 6 1.52×10 6 1.96×10 6 0 0 0 | 0 7.00×10 1 1.68×10 6 1.40×10 6 1.88×10 6 0 0 0 | 0 1.40×10 2 1.86×10 6 1.66×10 6 2.12×10 6 0 0 0 | 0 2.26×10 2 1.76×10 6 1.52×10 6 1.98×10 6 0 0 0 |
2, to the killing effect of test organisms
Bactericidal assay result: under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 30mi reaches more than 99.90% the average kill ratio to Candida albicans (ATCC10231).The results are shown in Table 1.
Table 1 Zaocys Bai Dianke is to the oidiomycetic killing effect of white
| Concentration | Average kill ratio and the scope (%) thereof of different action times (min) | |||
| 5 | 10 | 15 | 20 | |
| Stock solution 50% (v/v) | 99.75 (99.72-99.79) 99.64 | 99.82 (99.80-99.85) 99.70 | 99.88 (99.85-99.9) 99.77 | 99.92 (99.9-99.94) 99.83 |
| (99.62-99.67) | (99.68-99.73) | (99.74-99.82) | (99.80-99.86) |
Annotate: the average clump count 1.99 * 10 of positive controls
6Cfu/ml (scope: 1.72 * 10
6-2.24 * 10
6Cfu/ml) negative control group asepsis growth.
Four, conclusion:
1, the PBS solution that contains 3.0% tween 80+2.0% lecithin+0.5% sodium thiosulfate can neutralize fully to Zaocys Bai Dianke stock solution, and this nertralizer and neutralized reaction product do not have influence to Candida albicans (ATCC10231).
2, under 20 ± 2 ℃ of experimental conditions, Zaocys Bai Dianke stock solution effect 30min, the average kill ratio of Candida albicans (ATCC10231) is reached more than 99.90%.
| Name of product | Zaocys Bai Dianke | Connect the sample date | On 06 05th, 2002 |
| Inspection item | Bacteria quantified is killed test | Inspected the date | On 06 17th, 2002 |
One, equipment:
1. test strain: people's right forearm skin surface natural bacteria.
2. Zaocys Bai Dianke.
3. sample solution: the PBS solution that contains 3.0% tween 80+2.0% lecithin+0.5% sodium thiosulfate.
4, aseptic cotton carrier, shears etc.
5,0.03mol/LPBS liquid, culture medium etc.
6, aseptic measuring pipette, aseptic plate, test tube, constant incubator etc.
Two, method:
1, carries out according to " disinfection technology standard " third edition first fascicle.
2, check decontaminant concentration: Zaocys Bai Dianke stock solution.Disinfection treatment method: embrocate.Detecting sample number is 30 person-portions.
3, effect: 5min.
4, ambient temperature: 20 ℃ of relative temperatures: 60%
Three, result of the test:
Zaocys Bai Dianke stock solution effect 5min is 91.22% to the average kill ratio of the natural bacteria on people's right forearm skin surface, and the scope of killing is 90.00%-94.49%.The results are shown in Table 1.
Table 1 skin surface sterilization site test results
| Decontaminant concentration | (matched group) bacterium number before the sterilization | Sterilization back (test group) bacterium number | Killing rate |
| (cfu) | (cfu) | (％) | |
| Stock solution | 110 | 10 | 90.09 |
Annotate: the negative control group asepsis growth
Four, conclusion:
Under 20 ℃ of conditions, Zaocys Bai Dianke stock solution effect 5min, to the killing rate of natural bacteria on the hundred million body right forearm skin surfaces all 〉=90.00% average kill ratio is 91.22%.
(4) specific embodiment
The invention will be further described below in conjunction with embodiment:
The present invention is formulated by Chinese medicine Zaocys 70g, Mylabris 45g, Radix Angelicae Dahuricae 90g, Radix Arnebiae (Radix Lithospermi) 70g, Radix Sophorae Flavescentis 70g, Fructus Cnidii 70g, Fructus Psoraleae 90g.Decoct after Zaocys, Mylabris, the Radix Angelicae Dahuricae, Radix Arnebiae (Radix Lithospermi), Radix Sophorae Flavescentis, Fructus Cnidii, Fructus Psoraleae taken by weighing by above-mentioned weight, overanxious, concentrated, concentrate back and 60 ℃ of fused vaseline, lanoline mix homogeneously, adding is mix homogeneously behind the Borneolum Syntheticum of dissolve with ethanol and chlorhexidine acetate, gets product.
Claims (2)
1, a kind of medicine that uses the treatment leucoderma disease of Zaocys preparation is characterized in that being made by Chinese medicine Zaocys 70g, Mylabris 45g, Radix Angelicae Dahuricae 90g, Radix Arnebiae (Radix Lithospermi) 70g, Radix Sophorae Flavescentis 70g, Fructus Cnidii 70g, Fructus Psoraleae 90g.
2, a kind of medicine production method of using the treatment leucoderma disease of Zaocys preparation according to claim 1, decoct after it is characterized in that Zaocys, Mylabris, the Radix Angelicae Dahuricae, Radix Arnebiae (Radix Lithospermi), Radix Sophorae Flavescentis, Fructus Cnidii, Fructus Psoraleae taken by weighing by above-mentioned weight requirement, filter, concentrate, concentrate back and 60 ℃ of fused vaseline, lanoline mix homogeneously, adding is mix homogeneously behind the Borneolum Syntheticum of dissolve with ethanol and chlorhexidine acetate, gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100416319A CN100345559C (en) | 2005-01-17 | 2005-01-17 | Medicine from black snake for treating vitiligo |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100416319A CN100345559C (en) | 2005-01-17 | 2005-01-17 | Medicine from black snake for treating vitiligo |
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|---|---|
| CN1679826A CN1679826A (en) | 2005-10-12 |
| CN100345559C true CN100345559C (en) | 2007-10-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100416319A Active CN100345559C (en) | 2005-01-17 | 2005-01-17 | Medicine from black snake for treating vitiligo |
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| CN (1) | CN100345559C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199589B (en) * | 2007-12-13 | 2011-09-21 | 孙瑞龙 | Tincture medicament for treating face vifiligo |
| CN108619329A (en) * | 2018-07-25 | 2018-10-09 | 王益元 | A kind of Chinese medicinal ointment and preparation method thereof for treating leucoderma |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1312082A (en) * | 2000-03-07 | 2001-09-12 | 李祥德 | Leukoderma curing plaster |
| CN1368235A (en) * | 2001-02-01 | 2002-09-11 | 杨孟君 | Nano medicine for treating leukoderma and its preparing process |
| CN1370556A (en) * | 2001-02-26 | 2002-09-25 | 杨家龙 | Medicated snake wine and its prepn |
-
2005
- 2005-01-17 CN CNB2005100416319A patent/CN100345559C/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1312082A (en) * | 2000-03-07 | 2001-09-12 | 李祥德 | Leukoderma curing plaster |
| CN1368235A (en) * | 2001-02-01 | 2002-09-11 | 杨孟君 | Nano medicine for treating leukoderma and its preparing process |
| CN1370556A (en) * | 2001-02-26 | 2002-09-25 | 杨家龙 | Medicated snake wine and its prepn |
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| Publication number | Publication date |
|---|---|
| CN1679826A (en) | 2005-10-12 |
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