CN100344315C - Medicinal composition for promoting bone fracture healing and its preparing method - Google Patents
Medicinal composition for promoting bone fracture healing and its preparing method Download PDFInfo
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Abstract
The present invention relates to a medical composition for promoting fracture healing, which is characterized in that the medicament is prepared from the raw materials of the following proportion by weight: 1 to 20% of fortune's drynaria rhizome, 1 to 20% of glabrous sarcandra herb, 0 to 20% of Chinese teasel root and 0 to 30% of membranous milkvetch root. The present invention also discloses various preparation methods of the medical composition for promoting fracture healing. The medicine of the present invention is Chinese patent medicine with the advantages of scientific and reasonable prescription and obvious pharmacological action, and is used for accelerating fracture healing and reinforcing folding strength. The present invention has promotion action for the callus growth of a rat with experiment fractures through the indication of a pharmacological experiment, can reduce fracture healing time, and can obviously increase bone calcium content. Meanwhile, the present invention can reduce blood viscosity, improve blood circulation, and have strong inflammation and analgesic action. The preparation methods disclosed by the present invention have the advantages of advanced process and controllable quality. The medicine of the present invention, which is prepared by the preparation methods, has high content of effective components, and can further improve the curative effect.
Description
Technical field
The present invention relates to a kind of is the pharmaceutical composition of raw material with the Chinese herbal medicine, and particularly a kind of pharmaceutical composition that promotes union of fracture the invention still further relates to this preparation of drug combination method.
Background technology
At present the treatment of fractures method mainly is that surgery resets and fixes, but no matter how advanced surgical method is, the healing of general fracture also needed 3 months or the longer time, thereby made fracture person at very long a period of time disability even self care ability.Adopt the existence of the fractures of strong internal fixation method owing to stress shielding in addition, osteoporosis and osteanabrosis are obvious, and refracture easily takes place behind the taking-up internal fixation; The fractures that adopts the flexible fastening method easily causes fracture delayed union or disunion then because the epiphysis activity is difficult to control." Ningxia medical journal " 1999 the 21st volume 11 is interim to disclose one piece " clinical analysiss of " three-flower " bone-knitting powder treatment 128 example fracture ".The herbal mixture " three-flower " bone-knitting powder has detumescence, analgesic effect, can significantly promote the healing of fracturing." three-flower " bone-knitting powder is made up of Radix Notoginseng, Stigma Croci, Sanguis Draxonis etc. 18 flavor Chinese herbal medicine, in the side mainly Stigma Croci be rare medicinal herbs, and this medicine is powder, is unfavorable for that the patient takes.The Chinese patent application that application number is 02109322.9, name is called " Cortex torricelliae tiliifoliae " discloses the medicine of the treatment union of fracture of being made by multiple herbal raw material." Cortex torricelliae tiliifoliae " is made up of 20 flavor Chinese medicines such as Radix Angelicae Sinensis, Rhizoma Chuanxiong, Radix Paeoniae Rubra, and prescription is complicated.Also do not have prescription science, simple at present, pharmacological effect significantly promotes the Chinese patent medicine of union of fracture.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, and a kind of medicine that promotes union of fracture that a kind of compatibility is reasonable, prescription is simple, therapeutic effect is good is provided.
The present invention also provides the preparation method of this kind medicine.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of pharmaceutical composition that promotes union of fracture, is characterized in, it is the medicament of being made by following materials of weight proportions,
Rhizoma Drynariae 1～20, Herba Sarcandrae 1～20,
Radix Dipsaci 0～20, the Radix Astragali 0～30.
In technique scheme of the present invention, the Radix Dipsaci and the Radix Astragali are raw materials optionally, be that its raw material can be by Rhizoma Drynariae and Herba Sarcandrae 2 flavor medicine prescriptions, also can be any prescription simply in Rhizoma Drynariae, Herba Sarcandrae and Radix Dipsaci, the Radix Astragali, also can be Rhizoma Drynariae, Herba Sarcandrae, Radix Dipsaci and the common prescription of the Radix Astragali 4 flavors.
Technical problem to be solved by this invention can also realize by following technical scheme.Above-described pharmaceutical composition is characterized in, the weight proportion of each raw material is,
Rhizoma Drynariae 10, Herba Sarcandrae 10,
The Radix Dipsaci 10 and the Radix Astragali 20.
The present invention also provides a kind of preparation of drug combination method that promotes union of fracture, is characterized in that its step is as follows:
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stirring, make precipitation, cold preservation 24～48 hours filters, and filtrate is boiled, excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(3) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
Get the Radix Astragali, decoct with water 2～4 times, each 1～1.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1～3 time with ethanol precipitation, containing amount of alcohol in each solution is 75～85%, each all cold preservation was placed 10～24 hours, filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 5～6g, put coldly, promptly get Radix Astragali extract; Perhaps get the Radix Astragali, with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Reclaim ethanol and also be concentrated into the thick paste shape, add the water of 1～10 times of amount,, filter, be concentrated into every 1ml and be equivalent to crude drug 5～6g, put coldly, promptly get Radix Astragali extract through water precipitating 24～48 hours;
Perhaps,
(3) get Rhizoma Drynariae, the Radix Astragali, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is not hidden to there being alcohol, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae and Radix Astragali extract to there not being the alcohol flavor;
(4) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows:
(1) gets Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stirring, make precipitation, cold preservation 24～48 hours filters, and filtrate is boiled, excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(2) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, the Radix Astragali, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters; Filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae, Rhizoma Drynariae and Radix Astragali extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters; Filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows,
(1) gets Radix Dipsaci, the Radix Astragali, make solvent with 90%～95% ethanol, flood after 36～48 hours, speed with per minute 1-2ml is carried out percolation, collects percolate to total saponins class and filters out fully, perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get Radix Dipsaci and Radix Astragali extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, each cold preservation 24～48 hours; Filter, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows,
(1) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir adding ethanol makes extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, reclaimed ethanol to there not being the alcohol flavor.Through the polyamide adsorption treatment, water or 5%～25% ethanol liquid again with 60%～95% ethanol elution, reclaim ethanol towards post, concentrate, and get the Rhizoma Drynariae flavone extract, and in dry extract, the Rhizoma Drynariae general flavone content is not less than 50%;
(2) get Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get the Radix Dipsaci total saponin extracts, in dry extract, the Radix Dipsaci total saponin content is not less than 50%;
(3) get Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, add ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing the alcohol amount for the second time is 75%～85%, each cold preservation 24～48 hours, filter, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, the egg protein solution that adds an amount of new configuration, stir, make precipitation, cold preservation 24～48 hours, filter, filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filters filtrate recycling ethanol; Through the polyamide adsorption treatment, water or 5%～25% ethanol liquid again with 60%～95% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae flavone extract, and in dry extract, Herba Sarcandrae total flavones content is not less than 50%;
(4) get the Radix Astragali, decoct with water 2-4 time, each 1-2.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1-3 time with ethanol precipitation, and containing amount of alcohol in each solution is 75-85%, each all cold preservation was placed 10-48 hour, filtered, and reclaimed solvent; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate.Extracting solution is through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, is collected eluent behind water precipitating, reclaim solvent, get the Radix Astragali total saponins extract, in dry extract, Radix Astragali total saponins content is not less than 50%;
(5) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
The present invention also provides the preparation of drug combination method of another kind of promotion union of fracture, is characterized in, its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Get the Radix Astragali, decoct with water 2-4 time, each 1-2.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1-3 time with ethanol precipitation, and containing amount of alcohol in each solution is 75-85%, each all cold preservation was placed 10-48 hour, filtered, and reclaimed solvent; Perhaps get Radix Dipsaci, the Radix Astragali, with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Radix Dipsaci and Radix Astragali extractive solution are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get Radix Dipsaci, Radix Astragali total saponins extract, in dry extract, total saponin content is not less than 50%;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, each cold preservation 24～48 hours.Filter, filtrate recycling ethanol, through the polyamide adsorption treatment, water or 5%～25% ethanol liquid are towards post, with 60%～95% ethanol elution, reclaim ethanol again, concentrate, get Herba Sarcandrae and Rhizoma Drynariae flavone extract, in dry extract, general flavone content is not less than 50%;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
Above-mentioned any preparation method, the pharmaceutical formulation of its preparation can be an injection, during preparation, mixed extract adds 5～10 times of above waters for injection of amount, filters, cold preservation 24～48 hours, filtrate is regulated pH value to 7.5～9.0 with 20%NaOH solution, adds 0.05～0.15% active carbon, boiled 5～30 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 7.5～9.0, add the injection pharmaceutic adjuvant, make injection, or, make lyophilized injectable powder through the lyophilizing operation.
Because the Radix Dipsaci and the Radix Astragali are raw materials optionally in the technical scheme of pharmaceutical composition of the present invention, so, when its raw material is by Rhizoma Drynariae and Herba Sarcandrae 2 flavor medicine prescriptions, or during any prescription simply in Rhizoma Drynariae, Herba Sarcandrae and Radix Dipsaci, the Radix Astragali, this preparation of drug combination method then can be to choose the preparation method that the preparation process of corresponding raw material combines in above-mentioned any preparation method.
The dosage form of pharmaceutical composition of the present invention can be pharmaceutics the above comprise oral formulations, external preparation etc., as granule, tablet, capsule, soft capsule, drop pill, dispersible tablet, effervescent tablet, oral liquid, emplastrum in interior any dosage form.
Compared with prior art, medicine of the present invention is healing after a kind of prescription is scientific and reasonable, pharmacological action significantly is used to quicken to fracture and the Chinese patent medicine that strengthens rupture strength, pharmacological evaluation shows that it has facilitation, reduces healing time, can obviously improve calcium content of bone experimental fracture rat growth of spur, simultaneously can reduce whole blood viscosity, improve blood circulation, and stronger antiinflammatory, analgesic activity are arranged.Preparation method technology advanced person disclosed in this invention, quality controllable, with the medicine of the present invention that inventive method is prepared, its effective component content height, can further improve the curative effect of medicine of the present invention.
The specific embodiment
Embodiment 1.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: gram)
Rhizoma Drynariae 10, Herba Sarcandrae 10,
Radix Dipsaci 10, the Radix Astragali 20.
Adopt common process to make oral liquid.
Embodiment 2.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: gram)
Rhizoma Drynariae 10, Herba Sarcandrae 10,
Radix Dipsaci 10, the Radix Astragali 20.
Its preparation methods steps is as follows:
(1) gets Radix Dipsaci, make solvent, flood after 36 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1ml with 90% ethanol; Perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60% for the first time, containing for the second time the alcohol amount is 75%, and each cold preservation 24 hours filters, filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stir, make precipitation, cold preservation 24 hours, filter, filtrate is boiled, and excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(3) get Rhizoma Drynariae, decoct with water 2 times, 4 times of each amount of water are more than the amount, each 0.5 hour, filter, relative density is 1.10 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%, left standstill 24 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%, mixing left standstill 12 hours, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
Get the Radix Astragali, decoct with water 2 times, each 1 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1g, handles 1 time with ethanol precipitation, containing amount of alcohol in each solution is 75%, each all cold preservation was placed 10 hours, filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 5g, put coldly, promptly get Radix Astragali extract; Perhaps get the Radix Astragali, with 85%% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Reclaim ethanol and also be concentrated into the thick paste shape, add the water of 1 times of amount,, filter, be concentrated into every 1ml and be equivalent to crude drug 5g, put coldly, promptly get Radix Astragali extract through water precipitating 24 hours;
Perhaps,
(3) get Rhizoma Drynariae, the Radix Astragali, decoct with water 2 times, 4 times of each amount of water are more than the amount, each 0.5 hour, filter, relative density is 1.10 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%, left standstill 24 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%, mixing left standstill 12 hours, recovery ethanol gets Rhizoma Drynariae and Radix Astragali extract to there not being the alcohol flavor;
(4) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make tablet.
Embodiment 3.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: gram)
Rhizoma Drynariae 20, Herba Sarcandrae 20,
Radix Dipsaci 20, the Radix Astragali 30.
Its preparation methods steps is as follows:
(1) gets Radix Dipsaci, make solvent, flood after 48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 2ml with 95% ethanol; Perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 70% for the first time, containing for the second time the alcohol amount is 85%, and each cold preservation 48 hours filters, filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stir, make precipitation, cold preservation 48 hours, filter, filtrate is boiled, and excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(3) get Rhizoma Drynariae, decoct with water 4 times, 4 times of each amount of water are more than the amount, each 2 hours, filter, relative density is 1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 70%, left standstill 48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 85%, mixing left standstill 48 hours, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
Get the Radix Astragali, decoct with water 4 times, each 1.5 hours, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 2g, handles 3 times with ethanol precipitation, containing amount of alcohol in each solution is 85%, each all cold preservation was placed 24 hours, filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 6g, put coldly, promptly get Radix Astragali extract; Perhaps get the Radix Astragali, with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Reclaim ethanol and also be concentrated into the thick paste shape, add the water of 10 times of amounts,, filter, be concentrated into every 1ml and be equivalent to crude drug 6g, put coldly, promptly get Radix Astragali extract through water precipitating 48 hours;
Perhaps,
(3) get Rhizoma Drynariae, the Radix Astragali, decoct with water 4 times, 4 times of each amount of water are more than the amount, each 2 hours, filter, relative density is 1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 70%, left standstill 48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 85%, mixing left standstill 48 hours, recovery ethanol gets Rhizoma Drynariae and Radix Astragali extract to there not being the alcohol flavor;
(4) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make granule.
Embodiment 4.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 10, Herba Sarcandrae 10.
Its preparation methods steps is as follows:
(1) gets Herba Sarcandrae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60% for the first time, containing for the second time the alcohol amount is 75%, and each cold preservation 24 hours filters, filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stir, make precipitation, cold preservation 24 hours, filter, filtrate is boiled, and excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(2) get Rhizoma Drynariae, decoct with water 2 times, 4 times of each amount of water are more than the amount, each 0.5 hour, filter, relative density is 1.10 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%, left standstill 24 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%, mixing left standstill 12 hours, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make capsule.
Embodiment 5.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 20, Herba Sarcandrae 20.
Its preparation methods steps is as follows:
(1) gets Herba Sarcandrae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 70% for the first time, containing for the second time the alcohol amount is 85%, and each cold preservation 48 hours filters, filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stir, make precipitation, cold preservation 48 hours, filter, filtrate is boiled, and excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(2) get Rhizoma Drynariae, decoct with water 4 times, 4 times of each amount of water are more than the amount, each 2 hours, filter, relative density is 1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 70%, left standstill 48 hours, and filtered, filtrate recycling ethanol is not hidden to there being alcohol, stir to add ethanol and make extracting solution contain the alcohol amount to reach 85%, mixing left standstill 48 hours, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make soft capsule.Embodiment 6.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 1, Herba Sarcandrae 1,
Radix Dipsaci 1, the Radix Astragali 1.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1ml with 90% ethanol; Perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, the Radix Astragali, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction, filter, relative density was 1.5 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60, containing for the second time the alcohol amount is 75%, and each cold preservation 24 hours filters; Filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae, Rhizoma Drynariae and Radix Astragali extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make emplastrum.
Embodiment 7.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 15, Herba Sarcandrae 5,
Radix Dipsaci 5, the Radix Astragali 15.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 2ml with 95% ethanol; Perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, the Radix Astragali, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction, filter, relative density was 1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 70%, containing for the second time the alcohol amount is 85%, and each cold preservation 48 hours filters; Filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae, Rhizoma Drynariae and Radix Astragali extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make oral liquid.
Embodiment 8.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 10, Herba Sarcandrae 10, Radix Dipsaci 10.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1ml with 90% ethanol; Perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction, filter, relative density was 1.5 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60%, containing for the second time the alcohol amount is 75%, and each cold preservation 24 hours filters; Filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make dispersible tablet.
Embodiment 9.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 5, Herba Sarcandrae 15, Radix Dipsaci 20.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 2ml with 95% ethanol; Perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction, filter, relative density was 1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 70%, containing for the second time the alcohol amount is 85%, and each cold preservation 48 hours filters; Filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make injection.
Embodiment 10.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 8, Herba Sarcandrae 18,
Radix Dipsaci 12, the Radix Astragali 18.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, the Radix Astragali, make solvent with 90% ethanol, flood after 36 hours, speed with per minute 1ml is carried out percolation, collects percolate to total saponins class and filters out fully, perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, each return time 1 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get Radix Dipsaci and Radix Astragali extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction filters, and relative density was 1.5 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60%, containing the alcohol amount for the second time is 75%, each cold preservation 24 hours; Filter, filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make drop pill.
Embodiment 11.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 5, Herba Sarcandrae 15,
Radix Dipsaci 5, the Radix Astragali 25.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, the Radix Astragali, make solvent with 95% ethanol, flood after 48 hours, speed with per minute 2ml is carried out percolation, collects percolate to total saponins class and filters out fully, perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, each return time 2.5 hours filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get Radix Dipsaci and Radix Astragali extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction filters, and relative density was 1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 70%, containing the alcohol amount for the second time is 85%, each cold preservation 48 hours; Filter, filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the dried cream powder medicinal preparation for oral administration.
Embodiment 12.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 12, Herba Sarcandrae 8,
Radix Dipsaci 2, the Radix Astragali 12.
Its preparation methods steps is as follows,
(1) get Rhizoma Drynariae, decoct with water 2 times, 4 times of each amount of water are more than the amount, each 0.5 hour, filter, relative density is 1.10 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%, left standstill 24 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir adding ethanol makes extracting solution contain the alcohol amount to reach 75%, mixing left standstill 12 hours, reclaimed ethanol and did not hide to there being alcohol.Through the polyamide adsorption treatment, water or 5% ethanol liquid again with 60% ethanol elution, reclaim ethanol towards post, concentrate, and get the Rhizoma Drynariae flavone extract, and in dry extract, the Rhizoma Drynariae general flavone content is not less than 50%;
(2) get Radix Dipsaci, make solvent, flood after 36 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1ml with 90% ethanol; Perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get the Radix Dipsaci total saponin extracts, in dry extract, the Radix Dipsaci total saponin content is not less than 50%;
(3) get Herba Sarcandrae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction filters, relative density was 1.5 when filtrate was concentrated into 70 ℃ of heat surveys, add ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60%, containing the alcohol amount for the second time is 75%, each cold preservation 24 hours, filter, filtrate recycling ethanol, relative density is 1.1 when being concentrated into 70 ℃ of heat surveys, the egg protein solution that adds an amount of new configuration, stir, make precipitation, cold preservation 24 hours, filter, filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70%, places precipitation, filters filtrate recycling ethanol; Through the polyamide adsorption treatment, water or 5% ethanol liquid again with 60% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae flavone extract, and in dry extract, Herba Sarcandrae total flavones content is not less than 50%;
(4) get the Radix Astragali, decoct with water 2 times, each 1 hour, collecting decoction filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 1g, handles 1 time with ethanol precipitation, and containing amount of alcohol in each solution is 75%, and each all cold preservation was placed 10 hours, filtered, and reclaimed solvent; Perhaps with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate.Extracting solution is through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1 times of amount is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, is collected eluent behind water precipitating, reclaim solvent, get the Radix Astragali total saponins extract, in dry extract, Radix Astragali total saponins content is not less than 50%;
(5) mix the said extracted thing, add 8 times of above waters for injection of amount, filter, cold preservation 30 hours, filtrate is regulated pH value to 8.0 with 20%NaOH solution, add 0.10% active carbon, boiled 20 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 8.0, add the injection pharmaceutic adjuvant, make injection.
Embodiment 13.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 14, Herba Sarcandrae 4,
Radix Dipsaci 18, the Radix Astragali 14.
Its preparation methods steps is as follows,
(1) get Rhizoma Drynariae, decoct with water 4 times, 4 times of each amount of water are more than the amount, each 2 hours, filter, relative density is 1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 70%, left standstill 48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir adding ethanol makes extracting solution contain the alcohol amount to reach 85%, mixing left standstill 48 hours, reclaimed ethanol to there not being the alcohol flavor.Through the polyamide adsorption treatment, water or 25% ethanol liquid again with 95% ethanol elution, reclaim ethanol towards post, concentrate, and get the Rhizoma Drynariae flavone extract, and in dry extract, the Rhizoma Drynariae general flavone content is not less than 50%;
(2) get Radix Dipsaci, make solvent, flood after 48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 2ml with 95% ethanol; Perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, receive solvent, get the Radix Dipsaci total saponin extracts, in dry extract, the Radix Dipsaci total saponin content is not less than 50%;
(3) get Herba Sarcandrae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction filters, relative density was 1.35 when filtrate was concentrated into 70 ℃ of heat surveys, add ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 70%, containing the alcohol amount for the second time is 85%, each cold preservation 48 hours, filter, filtrate recycling ethanol, relative density is 1.3 when being concentrated into 70 ℃ of heat surveys, the egg protein solution that adds an amount of new configuration, stir, make precipitation, cold preservation 48 hours, filter, filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 80%, places precipitation, filters filtrate recycling ethanol; Through the polyamide adsorption treatment, water or 25% ethanol liquid again with 95% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae flavone extract, and in dry extract, Herba Sarcandrae total flavones content is not less than 50%;
(4) get the Radix Astragali, decoct with water 4 times, each 2.5 hours, collecting decoction filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 2g, handles 3 times with ethanol precipitation, and containing amount of alcohol in each solution is 85%, and each all cold preservation was placed 48 hours, filtered, and reclaimed solvent; Perhaps with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate.Extracting solution is through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 10 times of amounts is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, is collected eluent behind water precipitating, reclaim solvent, get the Radix Astragali total saponins extract, in dry extract, Radix Astragali total saponins content is not less than 50%;
(5) mix the said extracted thing, add 10 times of above waters for injection of amount, filter, cold preservation 48 hours, filtrate is regulated pH value to 9.0 with 20%NaOH solution, add 0.15% active carbon, boiled 30 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 9.0, add the injection pharmaceutic adjuvant, make injection.
Embodiment 14.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 20, Herba Sarcandrae 2,
Radix Dipsaci 2, the Radix Astragali 30.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1ml with 90% ethanol; Get the Radix Astragali, decoct with water 2 times, each 1 hour, collecting decoction filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 1g, handles 1 time with ethanol precipitation, and containing amount of alcohol in each solution is 75%, and each all cold preservation was placed 10 hours, filtered, and reclaimed solvent; Perhaps get Radix Dipsaci, the Radix Astragali, with 85% alcohol reflux 2 times, each alcoholic solution consumption is more than 4 times, and each return time 1 hour filters merging filtrate; Radix Dipsaci and Radix Astragali extractive solution are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get Radix Dipsaci, Radix Astragali total saponins extract, in dry extract, total saponin content is not less than 50%;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2 times, each 0.5 hour, each 4 times of amount of water, collecting decoction filters, and relative density was 1.5 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60%, containing the alcohol amount for the second time is 75%, each cold preservation 24 hours.Filter, filtrate recycling ethanol, through the polyamide adsorption treatment, water or 5% ethanol liquid again with 60% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae and Rhizoma Drynariae flavone extract, and in dry extract, general flavone content is not less than 50%;
(3) mix the said extracted thing, add 5 times of above waters for injection of amount, filter, cold preservation 24 hours, filtrate is regulated pH value to 7.5 with 20%NaOH solution, add 0.05% active carbon, boiled 5 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 7.5, add the injection pharmaceutic adjuvant, make lyophilized injectable powder.
Embodiment 15.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 16, Herba Sarcandrae 6,
Radix Dipsaci 16, the Radix Astragali 26.
Its preparation methods steps is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 2ml with 95% ethanol; Get the Radix Astragali, decoct with water 4 times, each 2.5 hours, collecting decoction filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 2g, handles 3 times with ethanol precipitation, and containing amount of alcohol in each solution is 85%, and each all cold preservation was placed 48 hours, filtered, and reclaimed solvent; Perhaps get Radix Dipsaci, the Radix Astragali, with 95% alcohol reflux 4 times, each alcoholic solution consumption is more than 4 times, and each return time 2.5 hours filters merging filtrate; Radix Dipsaci and Radix Astragali extractive solution are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 10 times of amounts, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get Radix Dipsaci, Radix Astragali total saponins extract, in dry extract, total saponin content is not less than 50%;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 4 times, each 2 hours, each 10 times of amount of water, collecting decoction filters, and relative density was 1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 70%, containing the alcohol amount for the second time is 85%, each cold preservation 48 hours.Filter, filtrate recycling ethanol, through the polyamide adsorption treatment, water or 25% ethanol liquid again with 95% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae and Rhizoma Drynariae flavone extract, and in dry extract, general flavone content is not less than 50%;
(3) mix the said extracted thing, add 6 times of above waters for injection of amount, filter, cold preservation 40 hours, filtrate is regulated pH value to 8.5 with 20%NaOH solution, add 0.15% active carbon, boiled 25 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 8.5, add the injection pharmaceutic adjuvant, make injection.
Embodiment 16.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 10, Herba Sarcandrae 10,
Radix Dipsaci 10.
The extracting method of pressing Rhizoma Drynariae, Herba Sarcandrae, Radix Dipsaci among the embodiment 13 extracts active ingredient, makes capsule.
Embodiment 17.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 10, Herba Sarcandrae 10,
The Radix Astragali 20.
The extracting method of pressing Rhizoma Drynariae, Herba Sarcandrae, the Radix Astragali among the embodiment 14 extracts active ingredient, makes tablet.
Embodiment 18.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 1, Herba Sarcandrae 1.
Make powder by common process of the prior art.
Embodiment 19.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 1, Herba Sarcandrae 20,
Radix Dipsaci 1.
Make tablet by common process of the prior art.
Embodiment 20.A kind of pharmaceutical composition that promotes union of fracture, it is the medicament of being made by following materials of weight proportions, (unit: kilogram)
Rhizoma Drynariae 20, Herba Sarcandrae 1,
The Radix Astragali 1.
Make capsule by common process of the prior art.
Press the pharmacodynamic study experiment (embodiment 21-24) of the dried cream powder of embodiment 11 preparations.
Embodiment 21.Dried cream powder is pressed effect experiment to the therapeutical effect of experimental fracture rat.
Experimental technique: get 50 of the male adult rats of SD, pentobarbital sodium anesthesia, the right lower limb of iodine tincture and alcohol disinfecting rat, laterally the sawing operation causes lower limb tibiofibula stage casing 3mm complete collyriculum, and art finishes sews up the incision, and will not cover wrapping, ip penicillin infection, 3d causes the rat fracture model continuously.Rat model is divided into 5 groups at random, 10 every group, i.e. dried cream powder high, medium and low dosage group, model group and positive drug group.Gastric infusion, every day 1 time, 30d continuously.Positive controls ig bone setting tablet 0.5g/kg, model group ig distilled water 10mL/kg, the high, medium and low dosage group of dried cream powder is respectively 0.25,0.5,1.0g/kg.Respectively at behind the medicine 15,30d, after each organizes rat anesthesia, adopt and take the X-ray sheet under 47kV, 100mA, 06s, the 90cm condition, adopt Shanghai City orthopedics department institute X-ray sheet evaluation criterion grading.
Experimental result: the result shows that the X-ray sheet of the 15th day each the dosage group of dried cream powder in fracture back has more obviously change, and the rat fracture end is fuzzy, and callus formation is arranged, and slight periosteal reaction is arranged, and obvious with the effect of heavy dose group, positive controls also has same purpose; And that model group only shows as fracture end is fuzzy, and slight periosteal reaction is arranged, indivedual callus as seen, but statistical test difference does not have significance.Fracture back 30d, there is more callus formation at the damaged place of dried cream powder group, and heavy dose of group has 7 and model group only to have 1 rat damaged place of fracturing to heal, and dried cream powder compares with model group, significant difference.(statistical method adopts grade preface value method).The results are shown in Table 1.
Table 1 dried cream powder is to the therapeutical effect of experimental fracture rat (X ± S)
| Group | Dosage (g/kg) | 15 days | 30 days | ||||||
| + | ++ | +++ | ++++ | + | ++ | +++ | ++++ | ||
| Dosage group low dose group in the model group bone setting tablet group high dose group | - 0.5 0.25 0.5 1.0 | 6 4 4 4 5 | 4 5 3 6 5 | 0 1 3 0 0 | 0 0 0 0 0 | 0 0 0 0 0 | 8 5 2 5 6 | 1 4 1 3 4 | 1 1 7 * 2 0 |
Annotate: compare with model group
*P＜0.05
Embodiment 22.Dried cream powder is to the therapeutical effect experiment of experimental fracture mice.
Experiment purpose: a kind of dried cream powder is to the therapeutical effect of experimental fracture mice
Experimental technique: get 75 of ICR mices, male, body weight 22～25g., hear the sound that fracture is disconnected simultaneously and feel that fracture is disconnected 90 ° of the right lower limb lower limb of mice doublings to the inside with hands, cause the mice fracture model, all do not wrap up fixingly, model mice is divided into 5 groups at random, 15 every group, be dried cream powder high, medium and low dosage group, model group and positive drug group.Gastric infusion, every day 1 time, 30d continuously.Positive controls ig bone setting tablet 1.0g/kg, model group ig distilled water 20mL/kg, the high, medium and low dosage group of dried cream powder is respectively 0.5,1.0,2.0g/kg.Respectively at after the administration 10,20, the every component of 30d criticizes and puts to death 5 mices, gets the right lower limb lower limb of mice femur, 10% formalin is fixed, and removes soft tissue, 10% nitric acid decalcification, paraffin section, haematoxylin-Yihong dyeing is observed the histological variation of callus.Curative effect judging standard: broken ends of fractured bone chondrocyte is ossified less than 50% (+), broken ends of fractured bone chondrocyte 50% (++) of ossify, broken ends of fractured bone chondrocyte ossify greater than 50% (+++).Each organizes the result and model group compares, and makes grade preface value method statistical analysis, sees Table 2.The result shows that dried cream powder can promote the ossified of mice bone stump chondrocyte, the healing that helps fracturing.
Table 2 dried cream powder is to the therapeutical effect of experimental fracture mice (X ± S)
| Group | Dosage (g/kg) | 10 days | 20 days | 30 days | ||||||
| + | ++ | +++ | + | ++ | +++ | + | ++ | +++ | ||
| Model group | - | 5 | 0 | 0 | 5 | 0 | 0 | 4 | 1 | 0 |
| The bone setting tablet group | 1.0 | 1 | 2 | 2 * | 0 | 3 | 2 ** | 0 | 1 | 4 ** |
| High dose group | 0.5 | 0 | 2 | 3 ** | 0 | 1 | 4 ** | 0 | 0 | 5 ** |
| Middle dosage group | 1.0 | 1 | 3 | 1 * | 0 | 3 | 2 ** | 0 | 1 | 4 ** |
| Low dose group | 2.0 | 1 | 4 | 0 * | 0 | 4 | 1 ** | 0 | 2 | 3 ** |
Annotate: compare with model group
*P＜0.05,
*P＜0.01
Embodiment 23.Dried cream powder causes the influence experiment of mice auricle swelling to Oleum Tiglii.
Experiment purpose: observe dried cream powder causes mice auricle swelling to Oleum Tiglii influence.
Experimental technique: get 50 of normal ICR mices, body weight 18～22g, male and female half and half.Be divided into 5 groups at random, every group of 10 mices, i.e. dried cream powder high, medium and low dosage group, model group and positive drug group.Gastric infusion, every day 1 time, continuous 7 days.Positive controls ig aspirin 0.11g/kg, model group ig distilled water 20mL/kg, the high, medium and low dosage group of dried cream powder is respectively 0.5,1.0,2.0g/kg.Behind the administration 40min on the 7th, be applied to two sides, ear front and back, a mice left side with 2% Oleum Tiglii 0.05ml, after causing scorching 4h, put to death mice, two ears about cutting along the auricle baseline, (diameter 9mm) takes off round auricle at same position respectively with card punch, scales/electronic balance weighing as auricular concha swelling degree, and calculates the swelling percentage rate with the difference of mice left and right sides auricular concha weight.
Experimental result: each group of dried cream powder all can alleviate Oleum Tiglii and cause the mice ear degree, reduces the swelling rate, has compared significant difference (p＜0.05, p＜0.01) with the blank group, and is similar to the aspirin group.Show that dried cream powder has stronger antiinflammatory action.The results are shown in Table 3.
The Rhizoma Pinelliae and capsule for treating gastropathy are to the influence of Oleum Tiglii induced mice ear swelling (X ± S)
| Group | Dosage (g/kg) | Number of animals (only) | Auricular concha swelling degree (mg) | The swelling rate |
| Dosage group low dose group in the blank group aspirin group high dose group | - 0.11 0.5 1.0 2.0 | 10 10 10 10 10 | 27.66±4.03 17.89±6.40 ** 18.40±7.97 ** 19.52±3.87 ** 21.84±6.28 * | 174.2±46.0 105.3±55.4 **108.0±56.2 **116.6±43.7 *129.1±42.4 * |
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
Embodiment 24.The dried cream powder Dichlorodiphenyl Acetate causes the influence experiment of mouse writhing.
Experiment purpose: observe the influence that the dried cream powder Dichlorodiphenyl Acetate causes mouse writhing.
Experimental technique: get 100 of normal ICR mices, body weight 18～22g, male and female half and half.Be divided into 5 groups at random, every group of 20 mices, i.e. dried cream powder high, medium and low dosage group, model group and positive drug group.Gastric infusion, every day 1 time, continuous 7 days.Positive controls ig aspirin 0.11g/kg, model group ig distilled water 20mL/kg, the high, medium and low dosage group of dried cream powder is respectively 0.5,1.0,2.0g/kg.Behind the administration 40min on the 7th, each is organized mouse peritoneal and only injects 0.6% acetic acid 0.2ml/, respectively organizes writhing response number of animals and the writhing response number of times that mice occurs in the 15min behind the observation injection acetic acid.
Experimental result: dried cream powder is respectively organized the body number of times of turning round of mice, and significantly to be lower than blank group suitable with model group, shows that dried cream powder can reduce the acetic acid abdominal cavity and stimulate and cause the number of times that mice pain is turned round body, has analgesic activity.The results are shown in Table 4.
Table 4 dried cream powder stimulates the influence cause the mice pain reaction (X ± S) to the acetic acid abdominal cavity
| Group | Dosage (g/kg) | Number of animals (only) | Do not turn round body number of animals (only) | Turn round body number of times (inferior) |
| Dosage group low dose group in the blank group aspirin group high dose group | - 0.11 0.5 1.0 2.0 | 20 20 20 20 20 | 0 6 ** 6 ** 5 * 2 | 37.4±6.1 12.6±10.4 ** 12.9±10.5 ** 18.7±15.9 ** 25.9±16.2 ** |
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
Press the pharmacodynamic study experiment (embodiment 25-28) of the injection of embodiment 15 preparations.
Embodiment 25.Injection is to the therapeutical effect experiment of experimental fracture rat.
Experiment purpose: observe the therapeutical effect of injection to experimental fracture rat.
Experimental technique: get 50 of the male adult rats of SD, pentobarbital sodium anesthesia, the right lower limb of iodine tincture and alcohol disinfecting rat, laterally the sawing operation causes lower limb tibiofibula stage casing 3mm complete collyriculum, and art finishes sews up the incision, and will not cover wrapping, ip penicillin infection, 3d causes the rat fracture model continuously.Rat model is divided into 5 groups at random, 10 every group, i.e. injection high, medium and low dosage group, model group and positive drug group.The tail vein injection administration, every day 1 time, 30d continuously.Positive controls FUFANG DANGGUI ZHUSHEYE im 0.5ml/kg, model group im normal saline 1.0mL/kg, the high, medium and low dosage group of injection is iv 0.25,0.5,1.0ml/kg respectively.Respectively at behind the medicine 15,30d, after each organizes rat anesthesia, adopt and take the X-ray sheet under 47kV, 100mA, 06s, the 90cm condition, adopt Shanghai City orthopedics department institute X-ray sheet evaluation criterion grading.
Experimental result: the result shows that the X-ray sheet of the 15th day each the dosage group of injection in fracture back has more obviously change, and the rat fracture end is fuzzy, and callus formation is arranged, and slight periosteal reaction is arranged, and obvious with the effect of heavy dose group, positive controls also has same purpose; And that model group only shows as fracture end is fuzzy, and slight periosteal reaction is arranged, indivedual callus as seen, but statistical test difference does not have significance.Fracture back 30d, there is more callus formation at the damaged place of injection group, and heavy dose of group has 7 and model group only to have 1 rat damaged place of fracturing to heal, and the injection group compares with model group, significant difference.(statistical method adopts grade preface value method).The results are shown in Table 1.
Table 1 injection is to the therapeutical effect of experimental fracture rat (X ± S)
| Group | Dosage (g/kg) | 15 days | 30 days | ||||||
| + | ++ | +++ | ++++ | + | ++ | +++ | ++++ | ||
| Dosage group low dose group in the model group positive drug group high dose group | - 0.5 0.25 0.5 1.0 | 6 4 4 3 5 | 4 5 4 7 5 | 0 1 2 0 0 | 0 0 0 0 0 | 0 0 0 0 0 | 8 5 1 4 6 | 1 4 2 4 4 | 1 1 7 * 2 0 |
Annotate: compare with model group
*P＜0.05
Embodiment 26.Injection is to the therapeutical effect experiment of experimental fracture mice.
Experiment purpose: injection is to the therapeutical effect of experimental fracture mice.
Experimental technique: get 75 of ICR mices, male, body weight 22～25g., hear the sound that fracture is disconnected simultaneously and feel that fracture is disconnected 90 ° of the right lower limb lower limb of mice doublings to the inside with hands, cause the mice fracture model, all do not wrap up fixingly, model mice is divided into 5 groups at random, 15 every group, be injection high, medium and low dosage group, model group and positive drug group.The tail vein injection administration, every day 1 time, 30d continuously.Positive controls FUFANG DANGGUI ZHUSHEYE im 1.0ml/kg, model group im normal saline 10mL/kg, the high, medium and low dosage group of injection is iv 0.5,1.0,2.0ml/kg respectively.Respectively at after the administration 10,20, the every component of 30d criticizes and puts to death 5 mices, gets the right lower limb lower limb of mice femur, 10% formalin is fixed, and removes soft tissue, 10% nitric acid decalcification, paraffin section, haematoxylin-Yihong dyeing is observed the histological variation of callus.Curative effect judging standard: broken ends of fractured bone chondrocyte is ossified less than 50% (+), broken ends of fractured bone chondrocyte 50% (++) of ossify, broken ends of fractured bone chondrocyte ossify greater than 50% (+++).Each organizes the result and model group compares, and makes grade preface value method statistical analysis, sees Table 2.The result shows that injection can promote the ossified of mice bone stump chondrocyte, the healing that helps fracturing.
Table 2 injection is to the therapeutical effect of experimental fracture mice (X ± S)
| Group | Dosage (g/kg) | 10 days | 20 days | 30 days | ||||||
| + | ++ | +++ | + | ++ | +++ | + | ++ | +++ | ||
| Model group | - | 5 | 0 | 0 | 5 | 0 | 0 | 4 | 1 | 0 |
| The positive drug group | 1.0 | 1 | 2 | 2 * | 0 | 3 | 2 ** | 0 | 1 | 4 ** |
| High dose group | 0.5 | 0 | 2 | 3 ** | 0 | 1 | 4 ** | 0 | 0 | 5 ** |
| Middle dosage group | 1.0 | 1 | 3 | 1 * | 0 | 3 | 2 ** | 0 | 1 | 4 ** |
| Low dose group | 2.0 | 1 | 4 | 0 * | 0 | 4 | 1 ** | 0 | 2 | 3 ** |
Annotate: compare with model group
*P＜0.05,
*P＜0.01
Embodiment 27.Injection causes the influence experiment of mice auricle swelling to Oleum Tiglii.
Experiment purpose: observe injection causes mice auricle swelling to Oleum Tiglii influence.
Experimental technique: get 50 of normal ICR mices, body weight 18～22g, male and female half and half.Be divided into 5 groups at random, every group of 10 mices, i.e. injection high, medium and low dosage group, model group and positive drug group.The tail vein injection administration, every day 1 time, continuous 7 days.Positive controls FUFANG DANGGUI ZHUSHEYE im 1.0ml/kg, model group im normal saline 10mL/kg, the high, medium and low dosage group of injection is im 0.5,1.0,2.0ml/kg respectively.Behind the administration 40min on the 7th, be applied to two sides, ear front and back, a mice left side with 2% Oleum Tiglii 0.05ml, after causing scorching 4h, put to death mice, two ears about cutting along the auricle baseline, (diameter 9mm) takes off round auricle at same position respectively with card punch, scales/electronic balance weighing as auricular concha swelling degree, and calculates the swelling percentage rate with the difference of mice left and right sides auricular concha weight.
Experimental result: each group of injection all can alleviate Oleum Tiglii and cause the mice ear degree, reduces the swelling rate, has compared significant difference (p＜0.05, p＜0.01) with the blank group, and is similar to the aspirin group.Show that injection has stronger antiinflammatory action.The results are shown in Table 3.
The Rhizoma Pinelliae and capsule for treating gastropathy are to the influence of Oleum Tiglii induced mice ear swelling (X ± S)
| Group | Dosage (g/kg) | Number of animals (only) | Auricular concha swelling degree (mg) | The swelling rate |
| Dosage group low dose group in the blank group FUFANG DANGGUI ZHUSHEYE group high dose group | - 1.0 0.5 1.0 2.0 | 10 10 10 10 10 | 27.66±4.03 17.89±6.40 ** 18.40±7.97 ** 19.52±3.87 ** 21.84±6.28 * | 174.2±46.0 105.3±55.4 **108.0±56.2 **116.6±43.7 *129.1±42.4 * |
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
Embodiment 28.The injection Dichlorodiphenyl Acetate causes the influence experiment of mouse writhing.
Experiment purpose: observe the influence that the injection Dichlorodiphenyl Acetate causes mouse writhing.
Experimental technique: get 100 of normal ICR mices, body weight 18～22g, male and female half and half.Be divided into 5 groups at random, every group of 20 mices, i.e. injection high, medium and low dosage group, model group and positive drug group.The tail vein injection administration, every day 1 time, continuous 7 days.Positive controls FUFANG DANGGUI ZHUSHEYE im 1.0ml/kg, model group im normal saline 10mL/kg, the high, medium and low dosage group of injection is im 0.5,1.0,2.0ml/kg respectively.Behind the administration 40min on the 7th, each is organized mouse peritoneal and only injects 0.6% acetic acid 0.2ml/, respectively organizes writhing response number of animals and the writhing response number of times that mice occurs in the 15min behind the observation injection acetic acid.
Experimental result: injection is respectively organized the body number of times of turning round of mice, and significantly to be lower than blank group suitable with model group, shows that injection can reduce the acetic acid abdominal cavity and stimulate and cause the number of times that mice pain is turned round body, has analgesic activity.The results are shown in Table 4.
Table 4 injection stimulates the influence cause the mice pain reaction (X ± S) to the acetic acid abdominal cavity
| Group | Dosage (g/kg) | Number of animals (only) | Do not turn round body number of animals (only) | Turn round body number of times (inferior) |
| Dosage group low dose group in the blank group FUFANG DANGGUI ZHUSHEYE group high dose group | - 1.0 0.5 1.0 2.0 | 20 20 20 20 20 | 0 0 5 3 2 | 38.9±6.7 18.0±14.6 ** 20.4±11.2 ** 23.6±10.6 ** 30.3±6.0 ** |
Annotate: compare with the blank group,
*P＜0.05,
*P＜0.01.
Claims (10)
1, a kind of pharmaceutical composition that promotes union of fracture is characterized in that, it is the medicament of being made by following materials of weight proportions,
Rhizoma Drynariae 1～20, Herba Sarcandrae 1～20,
Radix Dipsaci 0～20, the Radix Astragali 0～30.
2, pharmaceutical composition according to claim 1 is characterized in that, the weight proportion of each raw material is,
Rhizoma Drynariae 10, Herba Sarcandrae 10,
The Radix Dipsaci 10 and the Radix Astragali 20.
3, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows:
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stirring, make precipitation, cold preservation 24～48 hours filters, and filtrate is boiled, excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(3) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor; Get the Radix Astragali, decoct with water 2～4 times, each 1～1.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1～3 time with ethanol precipitation, containing amount of alcohol in each solution is 75～85%, each all cold preservation was placed 10～24 hours, filtered, and filtrate is concentrated into every 1ml and is equivalent to crude drug 5～6g, put coldly, promptly get Radix Astragali extract; Perhaps get the Radix Astragali, with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Reclaim ethanol and also be concentrated into the thick paste shape, add the water of 1～10 times of amount,, filter, be concentrated into every 1ml and be equivalent to crude drug 5～6g, put coldly, promptly get Radix Astragali extract through water precipitating 24～48 hours;
Perhaps,
(3) get Rhizoma Drynariae, the Radix Astragali, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae and Radix Astragali extract to there not being the alcohol flavor;
(4) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
4, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows:
(1) gets Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, stirring, make precipitation, cold preservation 24～48 hours filters, and filtrate is boiled, excessive egg protein is solidified, filter, filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, place precipitation, filter, filtrate recycling ethanol gets Herba Sarcandrae extract;
(2) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir to add ethanol and make extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, recovery ethanol gets Rhizoma Drynariae extract to there not being the alcohol flavor;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
5, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, the Radix Astragali, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters; Filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae, Rhizoma Drynariae and Radix Astragali extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
6, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get the Radix Dipsaci extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, added ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing for the second time the alcohol amount is 75%～85%, and each cold preservation 24～48 hours filters; Filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
7, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows,
(1) gets Radix Dipsaci, the Radix Astragali, make solvent with 90%～95% ethanol, flood after 36～48 hours, speed with per minute 1-2ml is carried out percolation, collects percolate to total saponins class and filters out fully, perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collected eluent, reclaim solvent, get Radix Dipsaci and Radix Astragali extract;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, each cold preservation 24～48 hours; Filter, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, adds the egg protein solution of an amount of new configuration, and stirring makes precipitation, and cold preservation 24～48 hours filters, and filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filter, filtrate recycling ethanol, Herba Sarcandrae and Rhizoma Drynariae extract;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
8, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows,
(1) get Rhizoma Drynariae, decoct with water 2～4 times, 4 times of each amount of water are more than the amount, each 0.5～2 hour, filter, relative density is 1.10-1.20 when being concentrated into 45 ℃ of heat surveys, stir adding ethanol down, mixing makes extracting solution contain the alcohol amount and reaches 55%～70%, left standstill 24～48 hours, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, stir adding ethanol makes extracting solution contain the alcohol amount to reach 75%～85%, mixing left standstill 12-48 hour, reclaimed ethanol to there not being the alcohol flavor.Through the polyamide adsorption treatment, water or 5%～25% ethanol liquid again with 60%～95% ethanol elution, reclaim ethanol towards post, concentrate, and get the Rhizoma Drynariae flavone extract, and in dry extract, the Rhizoma Drynariae general flavone content is not less than 50%;
(2) get Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Percolate or reflux extracting liquid are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get the Radix Dipsaci total saponin extracts, in dry extract, the Radix Dipsaci total saponin content is not less than 50%;
(3) get Herba Sarcandrae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction filters, relative density was 1.5～1.35 when filtrate was concentrated into 70 ℃ of heat surveys, add ethanol precipitation 2 times, make for the first time that to contain the alcohol amount be 60～70%, containing the alcohol amount for the second time is 75%～85%, each cold preservation 24～48 hours, filter, filtrate recycling ethanol, relative density is 1.1～1.3 when being concentrated into 70 ℃ of heat surveys, the egg protein solution that adds an amount of new configuration, stir, make precipitation, cold preservation 24～48 hours, filter, filtrate is boiled, and excessive egg protein is solidified, and filters; Filtrate adds ethanol, makes that to contain amount of alcohol be 70～80%, places precipitation, filters filtrate recycling ethanol; Through the polyamide adsorption treatment, water or 5%～25% ethanol liquid again with 60%～95% ethanol elution, reclaim ethanol towards post, concentrate, and get Herba Sarcandrae flavone extract, and in dry extract, Herba Sarcandrae total flavones content is not less than 50%;
(4) get the Radix Astragali, decoct with water 2-4 time, each 1-2.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1-3 time with ethanol precipitation, and containing amount of alcohol in each solution is 75-85%, each all cold preservation was placed 10-48 hour, filtered, and reclaimed solvent; Perhaps with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate.Extracting solution is through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, the water that adds 1～10 times of amount is crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, is collected eluent behind water precipitating, reclaim solvent, get the Radix Astragali total saponins extract, in dry extract, Radix Astragali total saponins content is not less than 50%;
(5) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
9, claim 1 or 2 described a kind of preparation of drug combination methods that promote union of fracture is characterized in that its step is as follows,
(1) gets Radix Dipsaci, make solvent, flood after 36～48 hours, carry out percolation, collect percolate to total saponins class and filter out fully with the speed of per minute 1-2ml with 90%～95% ethanol; Get the Radix Astragali, decoct with water 2-4 time, each 1-2.5 hour, collecting decoction, filter, filtrate is concentrated into every 1ml and is equivalent to crude drug 1～2g, handles 1-3 time with ethanol precipitation, and containing amount of alcohol in each solution is 75-85%, each all cold preservation was placed 10-48 hour, filtered, and reclaimed solvent; Perhaps get Radix Dipsaci, the Radix Astragali, with 85%～95% alcohol reflux 2～4 times, each alcoholic solution consumption is more than 4 times, and each return time 1～2.5 hour filters merging filtrate; Radix Dipsaci and Radix Astragali extractive solution are through aluminium oxide or activated carbon decolorizing, filter, reclaim ethanol and be concentrated into the thick paste shape, add the water of 1～10 times of amount, behind water precipitating, carry out ungrease treatment, degreaser crossed macroporous resin column absorption and with 40% above alcoholic solution eluting, collect eluent, reclaim solvent, get Radix Dipsaci, Radix Astragali total saponins extract, in dry extract, total saponin content is not less than 50%;
(2) get Herba Sarcandrae, Rhizoma Drynariae, decoct with water 2～4 times, each 0.5～2 hour, each 4～10 times of amount of water, collecting decoction, filter, filtrate is concentrated into 70 ℃ of heat when surveying, and relative density is 1.5～1.35, adds ethanol precipitation 2 times, makes that to contain the alcohol amount be 60～70% for the first time, containing for the second time the alcohol amount is 75%～85%, each cold preservation 24～48 hours.Filter, filtrate recycling ethanol, through the polyamide adsorption treatment, water or 5%～25% ethanol liquid are towards post, with 60%～95% ethanol elution, reclaim ethanol again, concentrate, get Herba Sarcandrae and Rhizoma Drynariae flavone extract, in dry extract, general flavone content is not less than 50%;
(3) mix the said extracted thing, concentrate, add pharmaceutic adjuvant, make the medicament of the described any dosage form of pharmaceutics.
10, according to any one described preparation method of claim 3-9, it is characterized in that, described medicament is an injection, during preparation, mixed extract adds 5～10 times of above waters for injection of amount, filters, cold preservation 24～48 hours, filtrate is regulated pH value to 7.5～9.0 with 20%NaOH solution, adds 0.05～0.15% active carbon, boils 5～30 minutes, filtration, ultrafiltration, reuse 20%NaOH solution is regulated pH value to 7.5～9.0, adds the injection pharmaceutic adjuvant, makes injection, or, make lyophilized injectable powder through the lyophilizing operation.
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| CN102743550B (en) * | 2012-07-12 | 2013-11-20 | 朱崇田 | Medicine composition for accelerating healing after fracture surgery, preparation method and application |
| CN104922192A (en) * | 2015-06-12 | 2015-09-23 | 史风 | Traditional Chinese medicine for treating fracture and preparation method thereof |
| CN108324760A (en) * | 2018-05-17 | 2018-07-27 | 黄美荣 | The Chinese herbal medicine for the treatment of fracture, injured bones and muscles |
| CN111821324A (en) * | 2020-07-31 | 2020-10-27 | 朱华 | Jianzhong fracture setting ointment for preventing and treating children's bone fracture diseases and preparation method thereof |
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| 实验性肾虚骨折愈合的生化学研究 冯坤，王健智，郭建刚，等，山东中医杂志，第19卷第1期 2001 * |
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