CN1283291C - Compound preparation for bone fracture and its preparation method - Google Patents
Compound preparation for bone fracture and its preparation method Download PDFInfo
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- CN1283291C CN1283291C CNB200410041020XA CN200410041020A CN1283291C CN 1283291 C CN1283291 C CN 1283291C CN B200410041020X A CNB200410041020X A CN B200410041020XA CN 200410041020 A CN200410041020 A CN 200410041020A CN 1283291 C CN1283291 C CN 1283291C
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Abstract
The present invention discloses a compound Chinese herbal preparation for curing fracture, and a preparation method thereof. The Chinese medicine is prepared from rhizoma drynariae, himalayan teasel root, Chinese fresh-water crab, angelica, catechu, dragon blood, olibanum, myrrh, ground beeltle and pyritum. The preparation method of the medicine comprises: (1) firstly, catechu, dragon blood, olibanum and myrrh are mixed, and then are pulverized into fine powder; (2) Chinese fresh-water crab and ground beeltle are treated the utility model coarse pulverization and decocted with water, and the decoction solution is concentrated; (3) rhizoma drynariae, himalayan teasel root and angelica are extracted with ethanol and concentrated; (4) pyritum is decocted with acetic acid and water and filtered, and the filter solution is concentrated; (5) the extracting solutions and the concentrated solutions are merged, and are prepared into a dry extract through vacuum drying; (6) the dry extract is pulverized, and then is uniformly mixed with the 4 raw medicinal power; the mixture is added with suitable supplementary materials, and can be prepared into granules, capsules, or tablets. The compound Chinese herbal preparation has obvious effect of restraining platelet aggregation, improving blood circulation, raising bone density, accelerating the deposition rate of mineral substances of bones, promoting the growth of bones, accelerating the healing of fracture, shortening healing time, etc., and is an ideal medicine for curing fracture.
Description
One, technical field
The present invention relates to a kind of Chinese medicine preparation, especially relate to a kind of compound Chinese medicinal preparation for the treatment of fracture and preparation method thereof.
Two, background technology
Fracture is a kind of commonly encountered diseases, and fracture is orthopaedic trauma common clinical, frequently-occurring disease, can damage social labor power, the serious harm human health.According to investigations, the prevalence of China's fracture is 1.37%, and annual morbidity is 0.28%, because China's population base is huge, the ill population of fracture that exists has 17,800,000 more than throughout the year.Chinese medicine has accumulated abundant clinical experience aspect the fracture preventing and treating, its total guiding theory is with " traumatic injury; all blood stasis is interior and do not loose also; the blood stasis of blood of not living then can not be gone; the stasis of blood is not gone then to roll over and can not be continued " and " stasis of blood is gone, new life, bone close ", the medicine that makes full use of the blood circulation promoting and blood stasis dispelling reunion of bone is corrected because of disconnected internal organs, meridians, the QI and blood dysfunction that causes of wound bone, reaches the purpose that promotes union of fracture.
The key of modern reported literature union of fracture is the generation and the fast breeding of callus, in its breeding, to make osteoblast more become active by functional irritation on the one hand, be to replenish necessary protein of growth of spur and trace element etc. continuously on the other hand, studies show that, Rhizoma Drynariae, Radix Dipsaci, have strong the kidney invigorating effect, reach immunity, the enhanced effect of cell stimulating activity regulated by the kidney invigorating.At present, patent medicine such as tcm clinical practice JIEGU QILI PIAN commonly used, but do not see that the user sea is the main compound preparation of forming medicine.
Three, summary of the invention
1, goal of the invention
The object of the present invention is to provide a kind ofly can promote union of fracture, quicken the extra large effective novel formulation in the side of containing of callus formation.
2, technical scheme
We recognize that in secular clinical practice no matter where fracture occurs in, its crucial pathogenesis all is " blood stasis stagnates ", and the meridians qi of zang-fu viscera of human body all will become empty by reality, therefore, and when medicine for treatment, must implement the principle of " eliminating evil must setting upright, do not set upright be not enough to eliminating evil ".The present invention according to the clinical practice of this theory and many decades, has the efficacious prescriptions medicine through screening just, and is developed into the compound Chinese medicinal preparation of treatment fracture, and its weight proportion is:
Rhizoma Drynariae 0.5～10g, Radix Dipsaci 0.2～8g, sea, side 1.0～20g, Radix Angelicae Sinensis 1.0～10g, catechu 0.3～5g, Sanguis Draxonis 0.5～5g, Olibanum (processed) 0.2～8g, Myrrha (processed) 0.2～8g, Eupolyphaga Seu Steleophaga 1.0～8g, Pyritum (calcined) 0.5～3g
More than the optimization weight proportion of each component be:
Rhizoma Drynariae 1.5g, Radix Dipsaci 1.2g, side extra large 2.0g, Radix Angelicae Sinensis 1.4g, catechu 0.35g, Sanguis Draxonis 0.95g, Olibanum (processed) 1.0g, Myrrha (processed) 1.0g, Eupolyphaga Seu Steleophaga 1.4g, Pyritum (calcined) 1.2g
The side separates: Rhizoma Drynariae, Radix Dipsaci, Fang Hai, Eupolyphaga Seu Steleophaga are monarch, and the bitter temperature of Rhizoma Drynariae nature and flavor is gone into the Liver and kidney warp, and the bitter temperature of Radix Dipsaci nature and flavor is also gone into kidney channel, and strong muscles and bones, with from kidney water with the training bone; The side extra large nature and flavor salty-cold, the removing blood stasis with potent drugs collateral dredging, hard masses softening and resolving, the Eupolyphaga Seu Steleophaga nature and flavor are salty-cold, removing blood stasis, the synthetism collateral dredging, with from the removing blood stasis with potent drugs collateral dredging with synthetism, four the flavor one, function reinforcement and elimination in combination, kidney tonifying synthetism.And be minister with Olibanum, Myrrha, Sanguis Draxonis, Olibanum, Myrrha are all suffering, hardship, using warming therapy product, promoting flow of QI and blood, muscles and tendons relaxing to alleviate pain, detumescence and promoting granulation, the Sanguis Draxonis nature and flavor are sweet, salty, flat, also have a merit of clots absorbing hemostasis and pain-relieving, help monarch to strengthen the promoting blood circulation and detumescence pain relieving jointly, the reunion of fractured tendons and bones that is highly advantageous to is an adjuvant drug with Radix Angelicae Sinensis, Pyritum again, and Radix Angelicae Sinensis is sweet, hot, warm, enrich blood and invigorate blood circulation, the Pyritum nature and flavor are hot flat, eliminating stasis to stop pain, reunion of bone, the two is assistant, auxilliary altogether the monarch and his subjects, more sending catechu is messenger drug, because of catechu nature and flavor hardship, puckery, cool, especially arrogate to oneself reason and hinder and to hold back damage, guide all medicines sick institute that goes directly.Full side has the effect of invigorating function of kidney and strengthening bone, promoting tissue regeneration by removing blood stasis, subduing swelling and relieving pain, reunion of fractured tendons and bones, cures mainly traumatic fracture.
This compound recipe adds suitable adjuvant and can be made into solid orally ingestible (capsule, tablet, granule etc.), and its preparation methods steps is:
(1) above ten flavors are got catechu, Sanguis Draxonis, Olibanum (processed), Myrrha (processed) mixing low temp and are pulverized, sieve fine powder;
(2) sea, side and Eupolyphaga Seu Steleophaga coarse pulverization decoct with water, and collecting decoction concentrates;
(3) Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis add alcohol reflux, merge extractive liquid,, and decompression recycling ethanol concentrates;
(4) Pyritum (calcined) adds the acetic acid decocting separately and boils, and filters, and merging filtrate concentrates;
(5) also will extract the concentrated solution mixing with above-mentioned side sea, Eupolyphaga Seu Steleophaga extraction concentrated solution and Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis, vacuum drying becomes dry extract;
(6) more above-mentioned dry extract is pulverized, four flavor crude drug powder mixings with step (1) add suitable adjuvant and can be made into granule, or be distributed into granule, or further are distributed into capsule or are pressed into tablet.
To the process conditions of above-mentioned preparation method, be further described as follows:
More than ten flavors, get catechu, Sanguis Draxonis, Olibanum (processed), Myrrha (processed) mixing low temp and pulverize, sieve fine powder (80 order).The coarse pulverization of Fang Haiyu Eupolyphaga Seu Steleophaga decocts with water 3 times, and each 2 hours, collecting decoction concentrated.Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis add 50% alcohol reflux 3 times, and each 2 hours, merge extractive liquid,, decompression recycling ethanol is concentrated into relative density 1.30 (60 ℃ of heat are surveyed).Pyritum (calcined) adds pH4 acetic acid decocting separately and boils 2 times, and each 2 hours, filter, merging filtrate concentrates.And extract concentrated solution and Rhizoma Drynariae, Radix Dipsaci with above-mentioned side sea, Eupolyphaga Seu Steleophaga, Radix Angelicae Sinensis extracts the concentrated solution mixing, 80 ℃, the 0.08MPa vacuum drying becomes dry extract.Above-mentioned dry extract is pulverized, and with preceding four flavor crude drug powder mixings, the centrifugal circumgyration of 30% ethanol is granulated, drying, and granulate (40 order), fill becomes capsule or adds appropriate amount of auxiliary materials to make granule or be pressed into tablet.
3. beneficial effect
We are with stretching the therapeutical effect of muscle bone-knitting capsule to rabbit and two kinds of fracture models of rat, and the laboratory observation result shows and has the following advantages:
(1) can the obvious suppression platelet aggregation, reduce blood viscosity, improve blood circulation.
(2) improve bone density, accelerate the sedimentation rate in bone ore deposit, thereby promote the healing of fracture.
(3) promote the bone trabecula hypertrophy, the fracture and injury place can be repaired rapidly, thereby quicken the healing of fracture, shorten healing time.
(4) can alleviate because the pain reaction due to inflammatory factor is oozed out in the fracture healing process.
Four, the specific embodiment
The embodiment of the compound Chinese medicinal preparation of (one) treatment fracture has:
Embodiment 1
The compound preparation of treatment fracture, its weight proportion is:
Rhizoma Drynariae 1.5g, Radix Dipsaci 1.2g, side extra large 2.0g, Radix Angelicae Sinensis 1.4g, catechu 0.35g, Sanguis Draxonis 0.95g, Olibanum (system) 1.0g, Myrrha (processed) 1.0g, Eupolyphaga Seu Steleophaga 1.4g, Pyritum (forging) 1.2g
Embodiment 2
Substantially the same manner as Example 1, but the compound recipe weight proportion is: Rhizoma Drynariae 2.0g, Radix Dipsaci 1.2g, side extra large 4.0g, Radix Angelicae Sinensis 2.0g, catechu 0.5g, Sanguis Draxonis 1.0g, Olibanum (system) 1.5g, Myrrha (processed) 1.5g, Eupolyphaga Seu Steleophaga 2.0g, Pyritum (forging) 1.0g
Embodiment 3
Substantially the same manner as Example 1, but the compound recipe weight proportion is: Rhizoma Drynariae 5g, Radix Dipsaci 4g, side extra large 4.0g, Radix Angelicae Sinensis 2g, catechu 0.5g, Sanguis Draxonis 1.5g, Olibanum (system) 2.0g, Myrrha (processed) 2.0g, Eupolyphaga Seu Steleophaga 2g, Pyritum (forging) 1.5g
Embodiment 4
Substantially the same manner as Example 1, but the compound recipe weight proportion is: Rhizoma Drynariae 10g, Radix Dipsaci 8g, side extra large 20g, Radix Angelicae Sinensis 1.4g, catechu 5g, Sanguis Draxonis 5g, Olibanum (system) 8g, Myrrha (processed) 8g, Eupolyphaga Seu Steleophaga 8g, Pyritum (forging) 3g
The preparation method of the compound preparation of (two) treatment fracture has:
Embodiment 5: stretch the muscle bone-knitting capsule
[prescription] Rhizoma Drynariae 121.5g, Radix Dipsaci 97.2g, side extra large 162.0g, Radix Angelicae Sinensis 113.4g, catechu 28.4g, Sanguis Draxonis 76.9g, Olibanum (system) 81.0g, Myrrha (processed) 81.0g, Eupolyphaga Seu Steleophaga 113.4g, Pyritum (forging) 97.2g make 1000 altogether.
[method for making] above ten flavors are got catechu, Sanguis Draxonis, Olibanum (system), the pulverizing of Myrrha (processed) mixing low temp, sieve fine powder (80 order).The coarse pulverization of Fang Haiyu Eupolyphaga Seu Steleophaga decocts with water 3 times, and each 2 hours, collecting decoction concentrated.Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis add 50% alcohol reflux 3 times, and each 2 hours, merge extractive liquid,, decompression recycling ethanol is concentrated into relative density 1.30 (60 ℃ of heat are surveyed).Pyritum (calcined) adds pH4 acetic acid decocting separately and boils 2 times, and each 2 hours, filter, merging filtrate concentrates.And extract concentrated solution and Rhizoma Drynariae, Radix Dipsaci with above-mentioned side sea, Eupolyphaga Seu Steleophaga, Radix Angelicae Sinensis extracts the concentrated solution mixing, 80 ℃, the 0.08MPa vacuum drying becomes dry extract.Above-mentioned dry extract is pulverized, and with preceding four flavor crude drug powder mixings, the centrifugal circumgyration of 30% ethanol is granulated, drying, and granulate (40 order), fill, promptly.
Embodiment 6: stretch the muscle tablet for curing fracture
Sea, [prescription] Rhizoma Drynariae 121.5g Radix Dipsaci 97.2g side 162.0g Radix Angelicae Sinensis 113.4g
Catechu 28.4g Sanguis Draxonis 76.9g Olibanum (system) 81.0g Myrrha (processed) 81.0g
Eupolyphaga Seu Steleophaga 113.4g Pyritum (forging) 97.2g makes 1000 altogether
[method for making] above ten flavors are got catechu, Sanguis Draxonis, Olibanum (system), the pulverizing of Myrrha (processed) mixing low temp, sieve fine powder (80 order).The coarse pulverization of Fang Haiyu Eupolyphaga Seu Steleophaga decocts with water 3 times, and each 2 hours, collecting decoction concentrated.Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis add 50% alcohol reflux 3 times, and each 2 hours, merge extractive liquid,, decompression recycling ethanol is concentrated into relative density 1.30 (60 ℃ of heat are surveyed).Pyritum (calcined) adds pH4 acetic acid decocting separately and boils 2 times, and each 2 hours, filter, merging filtrate concentrates.And extract concentrated solution and Rhizoma Drynariae, Radix Dipsaci with above-mentioned side sea, Eupolyphaga Seu Steleophaga, Radix Angelicae Sinensis extracts the concentrated solution mixing, 80 ℃, the 0.08MPa vacuum drying becomes dry extract.Above-mentioned dry extract is pulverized, and with preceding four flavor crude drug powder mixings, the centrifugal circumgyration of 30% ethanol is granulated, drying, and granulate (40 order) adds an amount of magnesium stearate, tabletting, promptly.
Embodiment 7: stretch muscle synthetism granule
Sea, [prescription] Rhizoma Drynariae 337.5g Radix Dipsaci 270.0g side 450.0g Radix Angelicae Sinensis 315.0g
Catechu 78.9g Sanguis Draxonis 213.6g Olibanum (system) 225.0g Myrrha (processed) 225.0g
Eupolyphaga Seu Steleophaga 315.0g Pyritum (forging) 270.0g makes 1000g altogether
[method for making] above ten flavors are got catechu, Sanguis Draxonis, Olibanum (system), the pulverizing of Myrrha (processed) mixing low temp, sieve fine powder (80 order).The coarse pulverization of Fang Haiyu Eupolyphaga Seu Steleophaga decocts with water 3 times, and each 2 hours, collecting decoction concentrated.Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis add 50% alcohol reflux 3 times, and each 2 hours, merge extractive liquid,, decompression recycling ethanol is concentrated into relative density 1.30 (60 ℃ of heat are surveyed).Pyritum (calcined) adds pH4 acetic acid decocting separately and boils 2 times, and each 2 hours, filter, merging filtrate concentrates.And extract concentrated solution and Rhizoma Drynariae, Radix Dipsaci with above-mentioned side sea, Eupolyphaga Seu Steleophaga, Radix Angelicae Sinensis extracts the concentrated solution mixing, 80 ℃, the 0.08MPa vacuum drying becomes dry extract.Above-mentioned dry extract is pulverized, and with preceding four flavor crude drug powder mixings, adds an amount of correctives, and the centrifugal circumgyration of ethanol is granulated, drying, and granulate (40 order), packing, promptly.
Embodiment 8 stretches the pharmacodynamics test of muscle bone-knitting capsule synthetism effect
The influence of a pair of rabbit fracture model
(1) experiment material:
Animal: white big ear rabbit, ♂, body weight 2-2.5kg is provided by animal reproduction field, Xian Tangshan Green Dragon mountain, Jiangning,
Instrument: Hitachi's 7020 automatic clinical chemistry analyzers, Japanese Hitachi company.
Bone densitometry instrument (Piximus bone densitometer), U.S. General ElectronicLunar company.
Medicine: stretch the muscle bone-knitting capsule, provide lot number by preparation research chamber, center, Nanjing University of Traditional Chinese Medicine ocean: 021017, people's dose every day is 12, and every is equivalent to crude drug amount 0.883g, and every gram extract powder is equivalent to crude drug amount 2.58g.
Radix Notoginseng powder provides lot number by Nanjing prepared slices of Chinese crude drugs factory: 020606
(2) experimental technique
(1) animal grouping: get 70 of rabbit, be divided into five groups at random, every group 14, be respectively model group (equal-volume distilled water), Radix Notoginseng powder positive drug group (1.26g/kg), stretch the heavy dose of group of muscle bone-knitting capsule (1.14g/kg, be equivalent to 2.94g crude drug/kg), (0.57g/kg is equivalent to 1.47g crude drug/kg) to stretch dosage group in the muscle bone-knitting capsule, (0.19g/kg is equivalent to 0.49g crude drug/kg) to stretch muscle bone-knitting capsule small dose group.
(2) making of fracture model: after adaptability is raised a week, after rabbit is pressed the intravenous anesthesia of 1ml/kg dosage with 3% pentobarbital sodium solution, both sides forelimb cropping, 75% alcohol disinfecting is drilled to the wide 3mm of being about with medical operation dental burr horizontal shape of epimere 1/3 intersection in the radius of both sides under aseptic condition, go deep into medullary cavity and form bone injury, skin suture begins gastric infusion, successive administration the back next day of performing the operation, get 7 that respectively organize in the rabbit on the 14th, 28 day after the administration, measure every index.
(3) bone densitometry: respectively at 14,28 days intercepting both sides radius, its length comprised bone injury and upper and lower each normal reach sclerotin, measures bone density with the U.S. Piximus bone densito-meter of LUNAR company analyzer, observes the union of fracture situation.
(4) blood biochemical measurement: with calcium, the phosphorus in the automatic clinical chemistry analyzer mensuration serum, indexs such as alkali phosphatase.
Pathomorphism: intercepting both sides radius, on its length comprises that bone injury reaches, following each normal reach sclerotin, place 10% formalin internal fixation, routine paraffin wax embedding after decalcification, slice thick 4～5 μ m, HE dyeing, according to the injury region connective tissue, cartilage, the degree that bone regeneration is repaired, be divided into Pyatyi, account for all hyperplastic tissues with osseous tissue and (comprise connective tissue, cartilage, osseous tissue) ratio is kept the score, and osseous tissue accounts for 1/5 and is designated as 1 fen, osseous tissue accounts for 2/5 and is designated as 2 fens, the rest may be inferred, and injury region is designated as 5 fens by osseous tissue substituent (osseous tissue accounts for 5/5) fully, and is divided into according to the reparation degree :+bone tissue restoration is slight, ++ moderate, +++severe, ++ ++ the basic reparation, ++ +++repair fully.Score value is high more, and the prompting curative effect is good more.
(3) result
(1) stretches of the influence of muscle bone-knitting capsule to rabbit fracture model bone density
Table 1 is stretched the muscle bone-knitting capsule to the influence of rabbit fracture model bone density (X ± SD)
| Group | Number of animals | Bone density (g/cm 3，14d) | Number of animals | Bone density (g/cm 3，28d) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 7 7 7 7 | 0.185±0.036 0.259±0.053 * 0.276±0.053 ** 0.209±0.027 0.247±0.056 * | 7 6 7 6 6 | 0.249±0.049 0.356±0.059 ** 0.297±0.016 * 0.301±0.018 * 0.252±0.061 |
Annotate: compare * P＜0.05 * * P＜0.01 with model group
Above experimental result shows: stretch the muscle bone-knitting capsule continuously after 14 days, the Radix Notoginseng powder group increases with the bone density of stretching muscle bone-knitting capsule small dose group, with model group significant difference (P＜0.05) is arranged relatively, also there were significant differences (P＜0.01) to stretch the heavy dose of group of muscle bone-knitting capsule.Administration after 28 days visible Radix Notoginseng powder group with model group notable difference (P＜0.01) is arranged relatively, stretch dosage group in the muscle bone-knitting capsule, heavy dose of group and model group more variant (P＜0.05).And the increase of 28 days bone density than 14 days, the result shows that stretching the muscle bone-knitting capsule can accelerate bone ore deposit sedimentation rate, improves bone density, promotes the healing of rabbit bone injury position.
(2) stretch of the influence of muscle bone-knitting capsule to rabbit fracture model serum levels of ALP, phosphorus, calcium
Table 2 is stretched the muscle bone-knitting capsule to the influence of rabbit fracture back serum levels of ALP, phosphorus, calcium (14d X ± SD)
| Group | Number of animals | ALP(U/L) | Phosphorus (mmol/L) | Calcium (mmol/L) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 7 7 7 7 | 108.00±36.18 125.00±52.39 136.29±50.31 110.43±60.43 138.71±52.32 | 12.40±1.65 12.47±2.80 12.24±1.68 13.11±1.72 12.15±2.16 | 11.47±0.51 12.06±0.80 11.19±0.94 11.11±0.77 10.64±0.79 |
Table 3 is stretched the muscle bone-knitting capsule to the influence of rabbit fracture back serum levels of ALP, phosphorus, calcium (28d X ± SD)
| Group | Number of animals | ALP(U/L) | Phosphorus (mmol/L) | Calcium (mmol/L) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 6 7 6 6 | 137.57±40.19 151.17±27.01 151.00±38.45 129.00±45.44 143.17±52.21 | 10.32±4.52 11.50±1.84 11.44±1.11 12.20±1.92 9.02±4.34 | 9.50±4.16 10.46±0.43 10.31±1.32 9.03±2.22 8.70±4.18 |
Above experimental result shows: each organizes medicine does not have obvious influence to ALP, phosphorus and calcium in the serum of rabbit fracture model 14 and 28 days, and each group and model group be unknown significance difference relatively.Point out oral can raise the calcium in the serum of 14,28 days differences of muscle bone-knitting capsule of stretching, phosphorus has content, but does not relatively have significant difference with model group.
(3) stretch of the histological examination of muscle bone-knitting capsule to rabbit fracture model fracture site
Table 4 is stretched the influence of muscle synthetism capsule glue to bone injury rabbit pathomorphism after 14 days
| Group | Bone tissue restoration | ||||
| + | ++ | +++ | ++++ | Divide equally | |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng group | 1/7 -- 2/7 3/6 1/6 | 4/7 5/7 3/7 2/6 3/6 | 1/7 1/7 2/7 1/6 1/6 | -- -- -- -- 1/6 | 1.86 2.00 2.14 2.00 2.50 |
Table 5 is stretched the influence of muscle synthetism capsule glue to bone injury rabbit pathomorphism after 28 days
| Group | Bone tissue restoration | |||||
| + | ++ | +++ | ++++ | +++++ | Divide equally | |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng group | -- -- -- -- -- | -- -- -- -- -- | 3/6 -- 1/7 2/6 2/6 | 2/6 2/5 -- 2/6 1/6 | 1/6 3/5 6/7 2/6 3/6 | 3.67 4.60 4.71 4.00 4.16 |
The fracture back is repaired and is shown as proliferation of fibrous tissue in early days, occurs chondrometaplasia later on, and the class bone matrix forms, and forms osseous tissue at last.Damaging back 14 days display model groups, to repair situation poor than other each groups, organizes and Radix Notoginseng is positive, and it is little to stretch between muscle synthetism high dose group, middle dosage group, three groups difference.Each is organized than other to stretch muscle synthetism low dose group damage back reparation.Damage and stretched muscle synthetism high dose group in back 28 days and repair preferably, most rabbits (6/7 example) bone wound place be the bone trabecula reparation entirely, and drug effect is higher than the bone injury of Radix Notoginseng processing; The situation that other each groups are repaired is followed successively by the positive group of Radix Notoginseng, stretches muscle synthetism low dose group, stretches dosage group in the muscle synthetism to difference by good.It is the poorest that model group is repaired situation, is starkly lower than other each experimental grouies.
(4) conclusion
The fracture back is repaired and is shown as proliferation of fibrous tissue in early days, occurs chondrometaplasia later on, and the class bone matrix forms, and forms osseous tissue at last.This experiment utilizes the rabbit fixed point to cause its bone injury, the research medicine is to the treatment recovery situation at its bone injury place behind the successive administration, result of study shows, stretch the muscle bone-knitting capsule continuously after 14,28 days, the Radix Notoginseng powder group increases with the bone density of stretching muscle bone-knitting capsule small dose group, with model group significant difference is arranged relatively, the increase of 28 days bone density than 14 days, the result shows that stretching the muscle bone-knitting capsule can accelerate bone ore deposit sedimentation rate, improves bone density, promotes rabbit bone injury hypertrophy.ALP, calcium in the rabbit anteserum but medicine can raise, but with model group relatively do not have obviously poor.Histopathology result shows, damage back osseous tissue is in histology's form of 14,28 days administration groups proliferation for repairing to some extent all, and the bone density that the muscle bone-knitting capsule improves the bone injury place is stretched in prompting, promotes the osseous tissue hypertrophy.
Two, to the influence of rat fracture model
(1) experiment material is identical with above-mentioned one.
(2) experimental technique
(1) animal grouping
Get 150 of rats, be divided into five groups at random, 30 every group, be respectively model group (equal-volume distilled water), Radix Notoginseng powder positive drug group (2.34g/kg), (2.21g/kg is equivalent to 5.70g crude drug/kg) to stretch the heavy dose of group of muscle bone-knitting capsule, stretch dosage group (1.11g/kg in the muscle bone-knitting capsule,, be equivalent to 2.86g crude drug/kg), stretch muscle bone-knitting capsule small dose group (0.37g/kg,, be equivalent to 0.95g crude drug/kg).
(2) making of fracture model:
After adaptability is raised a week, behind each group rat anesthesia,, cause its Fracture of femur model with the splenial bone pincers in its distal part of femur 1/3rd places.
(3) bone densitometry
Respectively at damage back 10 days, 20,30 days, get each Mus femur of its femur and survey its bone density, observe the osteogenesis situation of each group.
(4) blood biochemical measurement
Got blood in back 10 days, 20,30 days respectively at damage, measure serum calcium, phosphorus content and alkaline phosphatase activities with Hitachi's 7020 automatic clinical chemistry analyzers
(5) pathomorphism
Respectively at back 10 days of damage, 20, got its femur in 30 days, place 10% formalin internal fixation, routine paraffin wax embedding after decalcification, slice thick 4～5 μ m, HE dyeing, according to the injury region connective tissue, cartilage, the degree that bone regeneration is repaired, be divided into Pyatyi, account for all hyperplastic tissues with cartilage and osseous tissue and (comprise connective tissue, cartilage, osseous tissue) ratio is kept the score, cartilage and osseous tissue account for 1/5 and are designated as 1 fen, cartilage and osseous tissue account for 2/5 and are designated as 2 fens, and the rest may be inferred, and injury region is designated as 5 fens by cartilage and osseous tissue substituent (being that cartilage and osseous tissue account for 5/5) fully.Score value is high more, and the prompting curative effect is good more.To its pathomorphism histologic analysis, observe the osteogenesis situation of each group.
(3) result
(1) stretches of the influence of muscle bone-knitting capsule to rat fracture model bone density
Table 6 is stretched the muscle bone-knitting capsule to the influence of rat fracture model bone density (X ± SD)
| Group | Number of animals | Bone density (g/cm 3，10d) | Number of animals | Bone density (g/cm 3，20d) | Number of animals | Bone density (g/cm 3，30d) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 10 9 7 9 9 | 0.113±0.019 0.136±0.021 * 0.133±0.015 * 0.132±0.010 * 0.134±0.019 * | 7 9 10 9 9 | 0.118±0.023 0.170±0.043 * 0.165±0.038 ** 0.163±0.028 ** 0.151±0.019 ** | 9 8 9 8 8 | 0.154±0.034 0.195±0.024 * 0.200±0.026 ** 0.195±0.033 * 0.173±0.023 |
Annotate: compare * P＜0.05 * * P＜0.01 with model group
Above experimental result shows: successive administration is after 10 days, and visible Radix Notoginseng powder group increases with the bone density of stretching each dosage group of muscle bone-knitting capsule, with model group significant difference (P＜0.05) is arranged more all.After the administration 20 days, the bone density of Radix Notoginseng powder group increases, and with model group more variant (P＜0.05), stretching the large, medium and small dosage group of muscle bone-knitting capsule all has significant difference (P＜0.01).After the administration 30 days, Radix Notoginseng powder group and model group more variant (P＜0.05) are stretched in the muscle bone-knitting capsule dosage group and heavy dose of group relatively has significant difference (P＜0.01) with model group.Show after the rat fracture, stretch the sedimentation rate that the muscle bone-knitting capsule can be accelerated bone ore deposit composition, improved the density of bone, thereby promote the healing of fracture.
(2) stretch of the influence of muscle bone-knitting capsule to rat fracture model serum levels of ALP, phosphorus, calcium
Table 7 is stretched the muscle bone-knitting capsule to the clear ALP of rat fracture bleeding from anus, phosphorus, calcium influence (10d X ± SD)
| Group | Number of animals | ALP(U/L) | Phosphorus (mmol/L) | Calcium (mmol/L) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 9 9 10 10 10 | 264.22±86.12 290.22±28.27 273.30±79.00 276.90±94.72 271.70±64.41 | 15.24±1.84 17.02±3.01 14.43±1.45 15.84±1.41 16.15±2.14 | 9.38±0.54 9.90±0.53 9.81±0.57 9.92±0.30 9.72±0.76 |
Table 8 is stretched the muscle bone-knitting capsule to the clear ALP of rat fracture bleeding from anus, phosphorus, calcium influence (20d X ± SD)
| Group | Number of animals | ALP(U/L) | Phosphorus (mmol/L) | Calcium (mmol/L) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 8 8 10 9 9 | 264.75±86.54 210.00±57.79 234.80±50.94 250.44±83.35 294.78±157.80 | 14.76±1.97 17.05±2.50 14.50±1.15 15.19±1.36 15.84±2.28 | 8.83±0.29 8.84±0.54 9.02±0.48 9.30±0.50 9.06±0.52 |
Table 9 is stretched the muscle bone-knitting capsule to the clear ALP of rat fracture bleeding from anus, phosphorus, calcium influence (30d X ± SD)
| Group | Number of animals | ALP(U/L) | Phosphorus (mmol/L) | Calcium (mmol/L) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 8 8 7 9 8 | 230.88±45.84 201.25±49.70 198.86±54.30 227.44±39.59 202.75±36.04 | 9.03±0.70 8.90±0.50 8.60±0.39 8.78±0.33 8.78±0.29 | 16.65±4.20 14.73±2.02 14.61±1.55 14.95±0.99 14.30±1.88 |
Above experimental result shows: each organizes medicine does not have obvious influence to ALP, phosphorus and calcium in 10,20 and 30 days the serum of rat fracture model, each group is not seen difference with model group, and prompting medicine oral administration absorption back does not have influence to the content of the phosphorus in the blood, calcium and alkali phosphatase.
Pathomorphism:
Table 10 is stretched the influence of muscle bone-knitting capsule to the pathomorphology of rat bone damage model after 10 days
| Group | Bone tissue restoration | ||
| + | ++ | Divide equally | |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng group | 2/8 4/8 7/8 3/9 4/9 | -- -- -- -- 1/9 | 1.86 2.00 2.29 1.67 1.33 |
Table 11 is stretched the influence of muscle bone-knitting capsule to the pathomorphology of rat bone damage model after 20 days
| Group | Bone tissue restoration | ||
| + | ++ | Divide equally | |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng group | 8/9 5/7 3/7 4/6 2/4 | -- 1/7 3/7 1/6 1/4 | 2.10 2.43 2.80 2.33 2.50 |
Table 12 is stretched the influence of muscle bone-knitting capsule to the pathomorphology of rat bone damage model after 30 days
| Group | Bone tissue restoration | ||
| + | ++ | Divide equally | |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng group | 3/7 4/9 2/6 6/8 3/6 | 4/7 5/9 4/6 2/8 3/6 | 3.00 3.22 3.50 2.50 3.80 |
Different time sections is analyzed the demonstration of fracture repair situation with semi-quantitative method behind the fracture and injury: fractured back 10 days, stretching the heavy dose of group of muscle bone-knitting capsule is better than the positive group of Radix Notoginseng, stretches dosage group in the muscle bone-knitting capsule, stretches muscle bone-knitting capsule small dose group the curative effect of fracturing, fracture back shows after 20,30 days that to stretch muscle bone-knitting capsule small dose group curative effect best, and secondly the muscle bone-knitting capsule is heavy dose of to be organized in order to stretch.Stretch the muscle bone-knitting capsule in fracturing back 20 days, heavy dose of group and small dose group are better than model group reparation situation in the time of 30 days.It is relatively poor that model group different time sections behind fracture and injury is repaired situation, and every routine animal is divided equally and is starkly lower than most other experimental grouies.
(4) conclusion
It is more common clinically to fracture, and mainly is phalanges or bone trabecula in the effect of external force or suffers that under some pathologic condition integrity destroys.This experiment is reckoned the actual amount after a discount the therapeutical effect of testing the Research of Animal Model for Study medicine by rat bone, stretches the bone density that the muscle bone-knitting capsule can improve the rat fracture end, accelerates the sedimentation rate of bone ore deposit composition, promotes the healing of fracture, but ALP, phosphorus and calcium in the serum are not had obvious influence.Pathomorphology shows that stretching the muscle bone-knitting capsule can promote the bone trabecula hypertrophy, can repair the fracture and injury place rapidly.
Embodiment 9 stretches the muscle bone-knitting capsule to hemorheological influence
One, stretches the muscle bone-knitting capsule to the hemorheological influence of rabbit fracture model
(1) experiment material
Animal: white big ear rabbit, ♂, body weight 2-2.5kg,
Instrument: LG-R-80 blood viscosity tester, PAPER-1 type platelet aggregation and blood clotting analyzer, medicine: with embodiment 8
(2) experimental technique
(1) animal grouping: with embodiment 8
(2) fracture model preparation: after adaptability is raised a week, each organize rabbit with 3% pentobarbital sodium solution by the intravenous anesthesia of 1ml/kg dosage after, the forelimb cropping, 75% alcohol disinfecting is drilled to the wide 3mm of being about with medical operation dental burr horizontal shape of epimere 1/3 intersection in radius under aseptic condition, go deep into the bone injury of medullary cavity, splash into the penicillin solution antiinflammatory in wound, skin suture begins gastric infusion, successive administration the back next day of performing the operation.
(3) Determination of Blood Rheology: after administration the 14th, 28 day, the carotid artery blood sampling was surveyed platelet aggregation rate, whole blood, plasma viscosity.
(3) result
(1) stretches the muscle bone-knitting capsule to the hemorheological influence of rabbit fracture model
Table 13 is stretched the muscle bone-knitting capsule to the hemorheological influence of rabbit fracture model (14d X ± SD)
| Group | Number of animals | Whole blood viscosity | Plasma viscosity |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 7 7 7 7 | 3.37±1.57 3.84±1.95 2.63±0.27 2.97±0.54 2.96±1.41 | 1.97±1.03 2.40±1.07 1.97±0.80 2.51±1.52 1.94±0.77 |
Table 14 is stretched the muscle bone-knitting capsule to the hemorheological influence of rabbit fracture model (28d X ± SD)
| Group | Number of animals | Whole blood viscosity | Plasma viscosity |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 6 7 6 6 | 5.37±0.84 4.77±0.48 4.61±0.38 * 4.86±0.42 4.19±0.62 * | 1.87±0.13 2.00±0.21 1.94±0.10 2.02±0.06 1.86±0.10 |
Annotate: compare * P＜0.05 * * P＜0.01 with model group
Above experimental result shows, stretches the muscle bone-knitting capsule continuously after 14 days, and medicine is to the whole blood viscosity of rabbit fracture model, and plasma viscosity is obviously influence not, and each group and model group relatively do not have explicitly difference.As seen administration stretches the whole blood viscosity of heavy dose of group of muscle bone-knitting capsule and small dose group after 28 days, hematocrit value all has tangible reduction than model group, with model group more variant (P＜0.05).
Table 15 is stretched the muscle bone-knitting capsule to the influence of rabbit fracture model platelet aggregation rate (X ± SD)
| Group | Number of animals | Platelet aggregation rate (14d) | Number of animals | Platelet aggregation rate (28d) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 7 7 7 7 7 | 77.67±13.56 52.54±18.68 * 60.52±11.86 * 58.67±16.33 * 71.58±21.13 | 7 6 7 6 6 | 92.87±10.30 78.25±8.69 * 68.74±23.16 * 80.90±18.49 74.85±15.63 * |
Annotate: compare with model group
*P＜0.05
*P＜0.01
Above experimental result shows: when successive administration in the time of 14 days, positive drug Radix Notoginseng powder group and model group relatively have significant difference (P＜0.05), stretch in the muscle bone-knitting capsule dosage group and heavy dose of group relatively has significant difference (P＜0.05) with model group, after the administration 28 days, each group again with model group relatively, the Radix Notoginseng powder group, stretch muscle bone-knitting capsule small dose group and heavy dose of group variant (P＜0.05).Show that stretching the muscle bone-knitting capsule has inhibitory action to experimental bone injury rabbit platelet aggregation, but dose-effect relationship is not obvious, prompting is stretched the muscle bone-knitting capsule and can be reduced hematoblastic aggregation rate, blood circulation promoting.
(4) conclusion
This experiment utilizes the rabbit fixed point to cause its bone injury, the research medicine is to the treatment recovery situation at its bone injury place behind the successive administration, result of study shows, administration is in the time of 14 days, to its whole blood viscosity, plasma viscosity, not obviously influence, all tangible reduction is arranged but as seen administration stretches the whole blood viscosity of heavy dose of group of muscle bone-knitting capsule and small dose group after 28 days than model group, administration 14 and after 28 days, can improve the local blood supply, stretch muscle bone-knitting capsule height, in two dosage groups all can reduce hematoblastic aggregation rate, high dose group can reduce whole blood viscosity.
Two, stretch the muscle bone-knitting capsule to the hemorheological influence of rat fracture model
(1) experiment material
Animal: the SD rat, ♂, 120-150g is provided by Nanjing Medical University's Experimental Animal Center,
Instrument: LG-R-80 blood viscosity tester, Beijing Steellex Scientific Instrument Company
PAPER-1 type platelet aggregation and blood clotting analyzer, Beijing Steellex Scientific Instrument Company
Medicine: stretching the muscle bone-knitting capsule provides lot number by preparation research chamber, center, Nanjing University of Traditional Chinese Medicine ocean: 021017, and people's dose every day is 12, and every is equivalent to crude drug amount 0.883g, and every gram extract powder is equivalent to crude drug amount 2.58g.
Radix Notoginseng powder provides lot number 020606 by Nanjing prepared slices of Chinese crude drugs factory
(2) experimental technique
(1) experiment grouping: with embodiment 8 (two)
(2) preparation of (2) animal model:
After adaptability was raised a week, each group caused fracture model with the splenial bone pincers with its femur pinch off.Respectively organize gastric infusion (normal group is given isopyknic distilled water) next day, once a day, successive administration.
(3) hemorheological mensuration:
Respectively at the common carotid artery blood sampling in the 10th, 20 and 30 day after the administration, survey platelet aggregation rate, whole blood and plasma viscosity.
(3) experimental result
(1), stretches the muscle bone-knitting capsule to the hemorheological influence of rat fracture model
Table 16 is stretched the muscle bone-knitting capsule to the hemorheological influence of rat fracture model (10d X ± SD)
| Group | Number of animals | Whole blood viscosity | Plasma viscosity |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 9 9 10 10 10 | 4.54±0.91 4.81±0.97 5.16±0.86 5.17±0.69 5.03±0.74 | 1.99±1.26 1.45±0.34 2.00±0.77 1.76±0.37 1.57±0.27 |
Table 17 is stretched the muscle bone-knitting capsule to the hemorheological influence of rat fracture model (20d X ± SD)
| Group | Number of animals | Whole blood viscosity | Plasma viscosity |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 8 8 10 9 9 | 4.71±1.57 4.58±1.03 4.74±1.66 4.54±1.63 5.30±1.38 | 1.64±0.42 1.74±0.56 1.99±0.48 1.95±0.48 2.37±1.11 |
Table 18 is stretched the muscle bone-knitting capsule to the hemorheological influence of rat fracture model (30d X ± SD)
| Group | Number of animals | Whole blood viscosity | Plasma viscosity |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 8 8 7 9 8 | 6.16±0.46 6.09±0.99 6.21±1.57 5.89±0.87 6.87±2.17 | 1.75±0.09 1.81±0.08 1.90±0.15 * 1.78±0.06 2.00±0.52 |
Annotate: compare * P＜0.05 * * P＜0.01 with model group
Above experimental result shows: each organizes medicine does not have obvious influence to 10,20 and 30 days whole blood viscosity of rat fracture model, does not relatively have difference with model group.Plasma viscosity has remarkable rising except stretching the heavy dose of group of muscle bone-knitting capsule in 30 days than model group, outside model group more variant (P＜0.05), other respectively organize medicine does not have obvious influence to 10,20 and 30 days plasma viscosity of rat fracture model, does not relatively have difference with model group.The heavy dose of group of the muscle bone-knitting capsule hematocrit value of stretching behind positive drug Radix Notoginseng powder group behind the successive administration the 10th day time the and the successive administration the 20th day the time has remarkable rising than model group, with model group more variant (P＜0.05).Other respectively organizes medicine does not have obvious influence to 10,20 and 30 days hematocrit value of rat fracture model, does not relatively have difference with model group.
Table 19 is stretched the influence of muscle bone-knitting capsule to rat fracture model platelet aggregation rate
| Group | Number of animals | Platelet aggregation rate (10d) | Number of animals | Platelet aggregation rate (20d) | Number of animals | Platelet aggregation rate (30d) |
| Dosage group small dose group in the heavy dose of group of model group Radix Notoginseng powder group | 9 9 10 10 10 | 53.50±10.83 31.34±12.62 ** 43.16±19.34 38.66±12.26 * 30.80±15.98 * | 8 8 10 9 9 | 80.89±8.15 63.35±17.79 * 65.66±16.97 * 78.03±14.05 73.66±13.63 | 8 8 7 9 8 | 83.80±7.05 67.73±12.26 ** 71.83±8.95* 66.63±19.45 * 70.39±26.92 |
Annotate: compare with model group
*P＜0.05 * * P＜0.01
Above experimental result shows: in the time of 10 days, administration group and model group compare at the rat successive administration, and positive drug Radix Notoginseng powder group has evident difference (P＜0.01), stretches muscle bone-knitting capsule small dose group and middle dosage group variant (P＜0.05).In the time of 20 days, the administration group again with model group relatively, Radix Notoginseng powder group and stretch the heavy dose of group of muscle bone-knitting capsule variant (P＜0.05).In the time of 30 days and model group relatively, stretch therefrom dosage group variant (P＜0.05) of muscle bone-knitting capsule, the Radix Notoginseng powder group has notable difference (P＜0.01).Stretch each dosage group platelet aggregation rate of muscle bone-knitting capsule and model group ratio, certain decline is all arranged.Illustrate and stretch the muscle bone-knitting capsule by to rat platelet aggregation inhibitory action after fracturing.
Embodiment 10 stretches muscle bone-knitting capsule anti-inflammatory and analgesic effect
One, to the bullate influence of rat granuloma
Animal: the ICR rat, ♂, 120-150g is provided by Nanjing University of Traditional Chinese Medicine's Experimental Animal Center,
Instrument: SARTORIUS.The BP-310S electronic balance
Medicine: stretch the muscle bone-knitting capsule,
Aspirin,
Experimental technique:
Getting body weight is 50 of the healthy male rats of 120-150g, is divided into 5 groups at random, 10 every group.(1) model group (equal-volume distilled water); (2) aspirin group (200mg/kg); (2.21g/kg is equivalent to 5.70g crude drug/kg) to stretch the heavy dose of group of muscle bone-knitting capsule; Stretch dosage group in the muscle bone-knitting capsule (1.11g/kg,, be equivalent to 2.86g crude drug/kg); Stretch muscle bone-knitting capsule small dose group (0.37g/kg,, be equivalent to 0.95g crude drug/kg).After adaptability was raised, (30mg/kg) anaesthetized with pentobarbital sodium, behind 75% alcohol disinfecting of oxter, the rat left and right sides, implanted the cotton balls (handling with preceding penicillin and streptomycin mixed liquor 0.2ml) of sterilization 20mg.In gastric infusion next day, once a day, continuous five days, rat put to death in the 6th, cotton balls is taken out together with sheath on every side, remove fatty tissue, claim its Heavy Weight after drying to constant weight in 60 ℃ baking mutually, and calculate suppression ratio, the result calculates by following formula:
Suppression ratio=(dry weight of 1-administration group granulation tissue/blank group granulation tissue dry weight) * 100%
Experimental result sees Table 20:
Table 20 is stretched the muscle bone-knitting capsule to the bullate influence of rat granuloma (X ± SD)
| Group | Number of animals | Granuloma net weight (mg) | Suppression ratio (%) |
| Dosage group small dose group in the heavy dose of group of model group aspirin | 10 10 9 10 10 | 60.25±13.71 52.85±5.91 * 59.45±15.20 59.55±8.88 60.00±11.46 | —— 11.92 1.33 1.16 0.41 |
Annotate: compare with model group
*P＜0.05
Above experimental result shows: A Sisilin group and model group more variant (P＜0.05), but it is big to stretch the muscle bone-knitting capsule, in, small dose group and model group relatively do not have significant difference, suppression ratio to granuloma induced by implantation of cotton pellets is lower, points out the antiinflammatory action to granuloma induced by implantation of cotton pellets of stretching the muscle bone-knitting capsule not obvious.
Two, Dichlorodiphenyl Acetate causes the influence of mouse writhing experiment
Purpose: observation is stretched muscle bone-knitting capsule Dichlorodiphenyl Acetate induced mice writhing response and is had or not analgesic activity.
Animal: the ICR mice, ♀ ♂ half and half is provided by Nanjing Medical University's Experimental Animal Center, the animal production licence: moving accurate font size SCXK (Soviet Union) 2002-0031 of Soviet Union.
Medicine: stretch the muscle bone-knitting capsule,
Aspirin,
Acetic acid
Experimental technique:
Get body weight and be 61 of the healthy mices of 18～22g, male and female half and half are divided into 5 groups at random, (1) model group; (2) aspirin group (200mg/kg); (3) (3.12g/kg is equivalent to 8.05g crude drug/kg) to stretch the heavy dose of group of muscle bone-knitting capsule; (4) (1.56g/kg is equivalent to 4.02g crude drug/kg) to stretch dosage group in the muscle bone-knitting capsule; (5) (0.52g/kg is equivalent to 1.37g crude drug/kg) to stretch muscle bone-knitting capsule small dose group.After adaptability was raised, each organized the mouse stomach administration, once a day, and continuous three days.One hour (aspirin half an hour) after the last administration.Through lumbar injection 0.6% acetum 0.2ml/ only, timing is immediately observed and the writhing response number of record mice in 15min, and calculates the suppression ratio of medicine.The result calculates by following formula:
Suppression ratio=(the normal saline group is on average turned round body number-administration group and on average turned round the body number)/normal saline group is on average turned round body number * 100%
Experimental result:
Stretch the influence of muscle bone-knitting capsule Dichlorodiphenyl Acetate induced mice writhing response number of times
Table 21 is stretched the influence (X ± SD) of muscle bone-knitting capsule Dichlorodiphenyl Acetate induced mice writhing response number of times
| Group | Number of animals | Turn round the body number of times | Suppression ratio (%) |
| Dosage group small dose group in the heavy dose of group of normal group aspirin group | 12 13 12 12 12 | 46.6±14.7 17.3±16.9 ** 26.9±21.3 * 22.4±15.1 ** 31.7±17.3 * | 62.8 42.2 51.9 32.0 |
Annotate: compare with normal group
*P＜0.05
*P＜0.01
Above experimental result shows: aspirin group and stretch that dosage group and normal group relatively have significant difference (P＜0.01) in the muscle bone-knitting capsule, the mouse writhing reaction times that chemical pain is caused has the obvious suppression effect, stretch muscle bone-knitting capsule heavy dose of and small dose group and normal group more variant (P＜0.05), the mouse writhing reaction that causes is had certain inhibitory action.Illustrate that stretching the muscle bone-knitting capsule has significant analgesia role to the chemical pain model.
Three, Dichlorodiphenyl Acetate causes the influence of mice inflammation capillary permeability
Purpose: stretch muscle bone-knitting capsule Dichlorodiphenyl Acetate and cause the mice inflammation and make the capillary permeability increase have or not inhibitory action
Animal: the ICR mice, ♀ ♂ half and half is provided by Nanjing Medical University's Experimental Animal Center,
Instrument: centrifuge, BECKMEN company.
DU-640 nucleic acid-protein analyser, BECKMEN company.
Medicine: stretch the muscle bone-knitting capsule,
Aspirin,
Acetic acid,
Experimental technique:
Get 60 of ICR healthy mices, male and female half and half are divided into 5 groups at random, (1) model group (equal-volume distilled water); (2) aspirin group (200mg/kg); (3) (3.12g/kg is equivalent to 8.05g crude drug/kg) to stretch the heavy dose of group of muscle bone-knitting capsule; (4) (1.56g/kg is equivalent to 4.02g crude drug/kg) to stretch dosage group in the muscle bone-knitting capsule; (5) (0.52g/kg is equivalent to 1.37g crude drug/kg) to stretch muscle bone-knitting capsule small dose group.After adaptability was raised, each organized the mouse stomach administration, once a day, and continuous three days.One hour (aspirin half an hour) after the last administration.Inject 0.5% the blue liquid 0.01ml/g of ivens, the aqueous acetic acid 0.02ml/g of lumbar injection 0.5% at random through the tail vein.0.5 put to death animal after hour, with 10ml normal saline flushing abdominal cavity, cut off the abdominal cavity, collect peritoneal fluid, its liquid at the centrifugal 5min of 1000rpm, is got supernatant and surveyed its trap at the 590nm place with DU-640 nucleic acid-protein analyser.Record data see that the mouse peritoneal capillary permeability increase that the medicine Dichlorodiphenyl Acetate causes has or not inhibitory action.Experimental result sees Table 15:
Table 22 is stretched the influence (X ± SD) of muscle bone-knitting capsule Dichlorodiphenyl Acetate induced mice abdominal cavity capillary permeability
| Group | Number of animals | Absorbance |
| Dosage group small dose group in the heavy dose of group of normal group aspirin group | 10 11 11 10 10 | 0.208±0.072 0.157±0.044 * 0.217±0.092 0.190±0.052 0.199±0.061 |
Annotate: compare with normal group
*P＜0.05
Above experimental result shows: A Sisilin group and model group more variant (P＜0.05), but it is big to stretch the muscle bone-knitting capsule, in, small dose group and model group relatively do not have significant difference, and the absorbance and the normal group of stretching each dosage group of muscle bone-knitting capsule more significantly do not reduce more yet.The inhibition of inflammation that prompting is stretched due to the muscle bone-knitting capsule Dichlorodiphenyl Acetate is not remarkable.
Conclusion
Show by above anti-inflammatory and antalgic experimental result: stretch the muscle bone-knitting capsule and can not suppress capillary permeability, the muscle bone-knitting capsule is stretched in prompting does not have inhibitory action to the inflammation due to the bone injury; The propagation unrestraint effect of inflammation later stage fibroblast has the obvious suppression effect but stretch the muscle bone-knitting capsule to the mouse writhing reaction times that chemical pain causes, the muscle bone-knitting capsule is stretched in prompting can improve the pain reaction of fracture inflammatory factor due to discharging.
Claims (9)
1, a kind of compound preparation for the treatment of fracture is characterized in that it is formulated by following materials of weight proportions:
Rhizoma Drynariae 0.5～10g, Radix Dipsaci 0.2～8g, sea, side 1.0～20g, Radix Angelicae Sinensis 1.0～10g, catechu 0.3～5g, Sanguis Draxonis 0.5～5g, Olibanum (processed) 0.2～8g, Myrrha (processed) 0.2～8g, Eupolyphaga Seu Steleophaga 1.0～8g, Pyritum (calcined) 0.5～3g.
2, according to the compound preparation of the described treatment fracture of claim 1, it is characterized in that the optimization proportioning of each raw material components is in the compound recipe:
Rhizoma Drynariae 1.5g, Radix Dipsaci 1.2g, side extra large 2.0g, Radix Angelicae Sinensis 1.4g, catechu 0.35g, Sanguis Draxonis 0.95g, Olibanum (processed) 1.0g, Myrrha (processed) 1.0g, Eupolyphaga Seu Steleophaga 1.4g, Pyritum (calcined) 1.2g
3, according to the compound preparation of claim 1 or 2 described treatment fracture, after it is characterized in that adding suitable adjuvant, can be made into solid orally ingestible.
4, a kind of method for preparing the compound preparation of the described treatment fracture of claim 1, its preparation methods steps is:
(1) catechu in the above-mentioned prescription, Sanguis Draxonis, Olibanum (processed), Myrrha (processed) mixing low temp are pulverized, sieve fine powder;
(2) sea, side and Eupolyphaga Seu Steleophaga coarse pulverization, decoct with water, collecting decoction concentrates;
(3) with Rhizoma Drynariae, Radix Dipsaci, Radix Angelicae Sinensis, add alcohol reflux, merge extractive liquid,, decompression recycling ethanol concentrates;
(4) Pyritum (calcined) adds pH4 acetic acid decocting and boils, and filters, and merging filtrate concentrates;
(5) with the concentrated solution mixing of said extracted step (2), (3) and (4), vacuum drying becomes dry extract;
(6) more above-mentioned dry extract is pulverized, four flavor crude drug powder mixings with step (1) add suitable adjuvant and can be made into granule, or are distributed into granule; Or further be distributed into capsule; Or be pressed into tablet.
5, the preparation method of compound preparation according to claim 4 is characterized in that the fine powder in step (1) is 80 orders.
6, the preparation method of compound preparation according to claim 4 is characterized in that decocting with water 3 times each 2 hours in step (2).
7, the preparation method of compound preparation according to claim 4 is characterized in that adding 50% alcohol reflux 3 times in step (3), each 2 hours, be concentrated into relative density 1.30.
8, the preparation method of compound preparation according to claim 4 is characterized in that adding the acetic acid decocting and boils each 2 hours 2 times in step (4).
9, the preparation method of compound preparation according to claim 4 is characterized in that in step (5) 80 ℃ of vacuum drying temperature, 0.08MPa.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028835A (en) * | 2010-12-01 | 2011-04-27 | 介休市中医医院 | Chinese medicinal composition for treating fracture |
| CN107929469A (en) * | 2017-12-29 | 2018-04-20 | 张华丰 | A kind of Chinese medicine for treating fracture and preparation method thereof |
Families Citing this family (9)
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| CN100396325C (en) * | 2006-08-18 | 2008-06-25 | 南京中医药大学 | Compound medicine prepn. for treating osteoporosis, and its prepn. method |
| CN101850042B (en) * | 2010-06-15 | 2011-11-30 | 张贺堂 | Medicament for treating fractures |
| CN103961500A (en) * | 2013-02-04 | 2014-08-06 | 龚文山 | Method for rapid reunion of tendons and bones |
| CN105663440A (en) * | 2016-01-28 | 2016-06-15 | 张生禄 | Oral medicine for treating symptoms occurring in middle stage of fracture and soft tissue contusion |
| CN105641191A (en) * | 2016-01-28 | 2016-06-08 | 张生禄 | Orally-taken medicine for treating fracture and soft tissue contusion at preliminary stage |
| CN107007762A (en) * | 2017-06-16 | 2017-08-04 | 郜金元 | A kind of Chinese medicine composition, its preparation method and application for treating fracture |
| CN107998252A (en) * | 2017-12-13 | 2018-05-08 | 郭氏博信中医科技(北京)股份有限公司 | It is a kind of to treat fracture or the pharmaceutical composition of bone split and its preparation method and application |
| CN107982388A (en) * | 2017-12-27 | 2018-05-04 | 新乡医学院 | A kind of Chinese medicine composition for promoting union |
| CN109893582A (en) * | 2019-02-22 | 2019-06-18 | 通化师范学院 | A kind of compound Chinese medicinal preparation and preparation method thereof for treating traumatic injury |
-
2004
- 2004-06-18 CN CNB200410041020XA patent/CN1283291C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028835A (en) * | 2010-12-01 | 2011-04-27 | 介休市中医医院 | Chinese medicinal composition for treating fracture |
| CN107929469A (en) * | 2017-12-29 | 2018-04-20 | 张华丰 | A kind of Chinese medicine for treating fracture and preparation method thereof |
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| Publication number | Publication date |
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| CN1593583A (en) | 2005-03-16 |
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