CL2023002802A1 - Mosaicimo inducible. - Google Patents

Mosaicimo inducible.

Info

Publication number
CL2023002802A1
CL2023002802A1 CL2023002802A CL2023002802A CL2023002802A1 CL 2023002802 A1 CL2023002802 A1 CL 2023002802A1 CL 2023002802 A CL2023002802 A CL 2023002802A CL 2023002802 A CL2023002802 A CL 2023002802A CL 2023002802 A1 CL2023002802 A1 CL 2023002802A1
Authority
CL
Chile
Prior art keywords
plant
dna
floral
cell
editions
Prior art date
Application number
CL2023002802A
Other languages
English (en)
Inventor
Timothy Kelliher
Esteban Bortiri
Original Assignee
Syngenta Crop Protection Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection Ag filed Critical Syngenta Crop Protection Ag
Publication of CL2023002802A1 publication Critical patent/CL2023002802A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Permanent Magnet Type Synchronous Machine (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)

Abstract

Sistemas y métodos para producir una pluralidad de ediciones únicas en la semilla de una planta. En un ejemplo, un método comprende introducir en una células vegetal o un tejido vegetal un ácido nucleico que codifica una enzima de modificación del ADN; opcionalmente, un ácido nucleico que codifica al menos un ARN guía (ARNg); y una secuencia de sistema inducible, en el que la secuencia de sistema inducible se induce en un estadio de crecimiento deseado de la planta y o bien media en la expresión de la enzima de modificación del ADN en al menos una de una célula de primordios florales y un órgano reproductivo floral o bien transloca la enzima de modificación del ADN al núcleo; y (ii) media en una pluralidad de ediciones en el al menos uno de los primordios florales y el órgano reproductivo floral. El método puede incluir también regenerar una planta que tiene una pluralidad de semillas a partir de la célula vegetal o tejido vegetal, en el que la semilla contiene una pluralidad de ediciones únicas.
CL2023002802A 2021-03-24 2023-09-21 Mosaicimo inducible. CL2023002802A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163165224P 2021-03-24 2021-03-24

Publications (1)

Publication Number Publication Date
CL2023002802A1 true CL2023002802A1 (es) 2024-02-02

Family

ID=83396151

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2023002802A CL2023002802A1 (es) 2021-03-24 2023-09-21 Mosaicimo inducible.

Country Status (12)

Country Link
US (1) US20240167046A1 (es)
EP (1) EP4314276A1 (es)
JP (1) JP2024512050A (es)
KR (1) KR20230158660A (es)
CN (1) CN117043331A (es)
AR (1) AR125192A1 (es)
AU (1) AU2022245134A1 (es)
BR (1) BR112023019521A2 (es)
CA (1) CA3212095A1 (es)
CL (1) CL2023002802A1 (es)
IL (1) IL305892A (es)
WO (1) WO2022203927A1 (es)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723896B1 (en) * 1999-11-12 2004-04-20 The Rockefeller University Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants
US20160237451A1 (en) * 2013-09-30 2016-08-18 Regents Of The University Of Minnesota Conferring resistance to geminiviruses in plants using crispr/cas systems
EP3728581A4 (en) * 2017-12-22 2021-09-01 Bio-Rad Laboratories, Inc. CONTROL OF THE PHENOTYPE OF ORGANISMS WITH GENE TARGETING BY CRISPR / CAS
BR112021011656A2 (pt) * 2018-12-21 2021-11-30 Syngenta Participations Ag Edição de genes e indução haploide simultâneas

Also Published As

Publication number Publication date
AR125192A1 (es) 2023-06-21
BR112023019521A2 (pt) 2023-10-31
EP4314276A1 (en) 2024-02-07
US20240167046A1 (en) 2024-05-23
IL305892A (en) 2023-11-01
CN117043331A (zh) 2023-11-10
CA3212095A1 (en) 2022-09-29
KR20230158660A (ko) 2023-11-21
JP2024512050A (ja) 2024-03-18
WO2022203927A1 (en) 2022-09-29
AU2022245134A1 (en) 2023-09-14

Similar Documents

Publication Publication Date Title
Gonin et al. What makes adventitious roots?
Wodzicki Natural factors affecting wood structure
Nagata et al. Tobacco BY-2 cell line as the “HeLa” cell in the cell biology of higher plants
Chupeau et al. Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling
Dawood et al. Rapid flooding-induced adventitious root development from preformed primordia in Solanum dulcamara
Zhou et al. Desiccation treatment and endogenous IAA levels are key factors influencing high frequency somatic embryogenesis in Cunninghamia lanceolata (Lamb.) Hook
CN108893482B (zh) 丹参萜类合酶基因SmTPS8、其克隆引物、表达载体、催化产物及应用
US9957514B2 (en) Transformation of duckweed and uses thereof
Valimehr et al. A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma
Chaffey Why is there so little research into the cell biology of the secondary vascular system of trees?
Gomez et al. Laser microdissection and its application to analyze gene expression in arbuscular mycorrhizal symbiosis
Faisal et al. Artificial microRNA-based RNA interference and specific gene silencing for developing insect resistance in Solanum lycopersicum
Sabbadini et al. Factors affecting the regeneration, via organogenesis, and the selection of transgenic calli in the peach rootstock Hansen 536 (Prunus persica× Prunus amygdalus) to express an RNAi construct against PPV virus
AR096405A2 (es) Molécula de ácido nucleico que confiere tolerancia al herbicida dicamba, métodos para producir plantas tolerantes a dicamba y para métodos para controlar el crecimiento de malezas
Feeney et al. The role of Agrobacterium-mediated and other gene-transfer technologies in cannabis research and product development
Galatis et al. A new callose function: involvement in differentiation and function of fern stomatal complexes
Nagasato et al. Intercellular translocation of molecules via plasmodesmata in the multiseriate filamentous brown alga, Halopteris congesta (Sphacelariales, Phaeophyceae)
Krishnamurthy Growth and development in plants (textbook series: 21st century biology and agriculture)
CL2021002224A1 (es) Composiciones y métodos para impulsar la diversidad de eventos t1.
CL2023002802A1 (es) Mosaicimo inducible.
Campanoni et al. A generalized method for transfecting root epidermis uncovers endosomal dynamics in Arabidopsis root hairs
KR101374182B1 (ko) 버드나무류의 재분화방법 및 이를 향상시키는 방법
Gotté et al. Endoplasmic reticulum body–related gene expression in different root zones of Arabidopsis isolated by laser‐assisted microdissection
CN107312794B (zh) 玫瑰RrNUDX1基因在改变植物花期中的应用
AR046587A1 (es) Elementos regulatorios de actina para su uso en plantas