CL2021002224A1 - Composiciones y métodos para impulsar la diversidad de eventos t1. - Google Patents

Composiciones y métodos para impulsar la diversidad de eventos t1.

Info

Publication number
CL2021002224A1
CL2021002224A1 CL2021002224A CL2021002224A CL2021002224A1 CL 2021002224 A1 CL2021002224 A1 CL 2021002224A1 CL 2021002224 A CL2021002224 A CL 2021002224A CL 2021002224 A CL2021002224 A CL 2021002224A CL 2021002224 A1 CL2021002224 A1 CL 2021002224A1
Authority
CL
Chile
Prior art keywords
plant
methods
flower
cell
fmos
Prior art date
Application number
CL2021002224A
Other languages
English (en)
Inventor
Zhongying Chen
Timothy Kelliher
Julie Green
Wan Shi
Guozhu Tang
Jiang Li
Emily Scorgie
Original Assignee
Syngenta Crop Protection Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection Ag filed Critical Syngenta Crop Protection Ag
Publication of CL2021002224A1 publication Critical patent/CL2021002224A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8233—Female-specific, e.g. pistil, ovule

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Sistemas y métodos para producir una pluralidad de ediciones únicas en la semilla T1 de una planta. En un ejemplo, un método comprende transformar al menos un casete de expresión en una célula vegetal o un tejido vegetal. El al menos un casete de expresión puede comprender un ácido nucleico que codifica una enzima de modificación de ADN; opcionalmente, un ácido nucleico que codifica al menos un ARN guía (ARNg); y una secuencia reguladora de mosaico floral (FMOS), donde la secuencia reguladora de FMOS (i) media la expresión de la enzima de modificación del ADN en al menos una de célula de primordios florales y un órgano reproductor floral, y (ii) media una pluralidad de ediciones en al menos uno de los primordios florales y el órgano reproductor floral. El método también puede incluir regenerar la célula vegetal o tejido vegetal en una planta T0 que tiene una pluralidad de semillas T1, donde la semilla T1 contiene una pluralidad de ediciones únicas.
CL2021002224A 2019-02-25 2021-08-20 Composiciones y métodos para impulsar la diversidad de eventos t1. CL2021002224A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2019076062 2019-02-25

Publications (1)

Publication Number Publication Date
CL2021002224A1 true CL2021002224A1 (es) 2022-03-04

Family

ID=72238559

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2021002224A CL2021002224A1 (es) 2019-02-25 2021-08-20 Composiciones y métodos para impulsar la diversidad de eventos t1.

Country Status (9)

Country Link
US (1) US20220145312A1 (es)
EP (1) EP3930449A4 (es)
CN (1) CN113544277A (es)
AU (1) AU2020228277A1 (es)
BR (1) BR112021016761A2 (es)
CA (1) CA3130673A1 (es)
CL (1) CL2021002224A1 (es)
IL (1) IL285379A (es)
WO (1) WO2020176412A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UY39752A (es) * 2021-05-04 2022-11-30 Syngenta Crop Protection Ag Moléculas de ácido nucleico para conferir propiedades insecticidas a plantas

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10448588B2 (en) * 2013-03-15 2019-10-22 Syngenta Participations Ag Haploid induction compositions and methods for use therefor
EP3095870A1 (en) * 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
MX2018005968A (es) * 2015-11-18 2018-08-29 Syngenta Participations Ag Composiciones de induccion de haploides y metodos de uso de las mismas.
WO2017096237A1 (en) * 2015-12-02 2017-06-08 Ceres, Inc. Methods for genetic modification of plants
KR102630763B1 (ko) * 2016-12-02 2024-01-26 신젠타 파티서페이션즈 아게 동시 유전자 편집 및 반수체 유도
CN108486145A (zh) * 2018-02-12 2018-09-04 中国科学院遗传与发育生物学研究所 基于CRISPR/Cas9的植物高效同源重组方法

Also Published As

Publication number Publication date
WO2020176412A3 (en) 2020-10-01
CN113544277A (zh) 2021-10-22
US20220145312A1 (en) 2022-05-12
EP3930449A2 (en) 2022-01-05
EP3930449A4 (en) 2022-11-30
IL285379A (en) 2021-09-30
CA3130673A1 (en) 2020-09-03
BR112021016761A2 (pt) 2021-11-16
WO2020176412A2 (en) 2020-09-03
AU2020228277A1 (en) 2021-09-02

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