BR112023019521A2 - Mosaicismo indutível - Google Patents

Mosaicismo indutível

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Publication number
BR112023019521A2
BR112023019521A2 BR112023019521A BR112023019521A BR112023019521A2 BR 112023019521 A2 BR112023019521 A2 BR 112023019521A2 BR 112023019521 A BR112023019521 A BR 112023019521A BR 112023019521 A BR112023019521 A BR 112023019521A BR 112023019521 A2 BR112023019521 A2 BR 112023019521A2
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Brazil
Prior art keywords
plant
floral
inducible
modifying enzyme
dna modifying
Prior art date
Application number
BR112023019521A
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English (en)
Inventor
Esteban Bortiri
Timothy Kelliher
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Syngenta Crop Protection Ag
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Publication date
Application filed by Syngenta Crop Protection Ag filed Critical Syngenta Crop Protection Ag
Publication of BR112023019521A2 publication Critical patent/BR112023019521A2/pt

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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C07K2319/00—Fusion polypeptide
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    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
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  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Yarns And Mechanical Finishing Of Yarns Or Ropes (AREA)
  • Permanent Magnet Type Synchronous Machine (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)

Abstract

mosaicismo indutível. a presente invenção refere-se a sistemas e métodos para produzir uma pluralidade de edições únicas em uma semente de planta. em um exemplo, um método compreende a introdução em uma célula vegetal ou em um tecido vegetal de um ácido nucleico que codifica uma enzima de modificação de dna; opcionalmente, um ácido nucleico que codifica pelo menos um rna guia (grna); e uma sequência de sistema induzível, em que a sequência de sistema induzível é induzida no estágio de crescimento desejado de uma planta e tanto medeia a expressão da enzima de modificação de dna em pelo menos uma dentre uma célula de primórdio floral e um órgão reprodutivo floral ou transloca a enzima de modificação de dna para o núcleo; e (ii) medeia uma pluralidade de edições em pelo menos um dos primórdios florais e do órgão reprodutivo floral. o método também pode incluir a regeneração da célula vegetal ou tecido vegetal em uma planta tendo uma pluralidade de sementes, em que a semente contém uma pluralidade de edições únicas.
BR112023019521A 2021-03-24 2022-03-17 Mosaicismo indutível BR112023019521A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163165224P 2021-03-24 2021-03-24
PCT/US2022/020690 WO2022203927A1 (en) 2021-03-24 2022-03-17 Inducible mosaicism

Publications (1)

Publication Number Publication Date
BR112023019521A2 true BR112023019521A2 (pt) 2023-10-31

Family

ID=83396151

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023019521A BR112023019521A2 (pt) 2021-03-24 2022-03-17 Mosaicismo indutível

Country Status (12)

Country Link
US (1) US20240167046A1 (pt)
EP (1) EP4314276A1 (pt)
JP (1) JP2024512050A (pt)
KR (1) KR20230158660A (pt)
CN (1) CN117043331A (pt)
AR (1) AR125192A1 (pt)
AU (1) AU2022245134A1 (pt)
BR (1) BR112023019521A2 (pt)
CA (1) CA3212095A1 (pt)
CL (1) CL2023002802A1 (pt)
IL (1) IL305892A (pt)
WO (1) WO2022203927A1 (pt)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6723896B1 (en) * 1999-11-12 2004-04-20 The Rockefeller University Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants
WO2015048707A2 (en) * 2013-09-30 2015-04-02 Regents Of The University Of Minnesota Conferring resistance to geminiviruses in plants using crispr/cas systems
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
EP3899016A4 (en) * 2018-12-21 2022-10-05 Syngenta Participations AG SIMULTANEOUS GENOMIC EDITING AND HAPLOID INDUCTION

Also Published As

Publication number Publication date
CA3212095A1 (en) 2022-09-29
EP4314276A1 (en) 2024-02-07
CL2023002802A1 (es) 2024-02-02
US20240167046A1 (en) 2024-05-23
AR125192A1 (es) 2023-06-21
WO2022203927A1 (en) 2022-09-29
AU2022245134A1 (en) 2023-09-14
IL305892A (en) 2023-11-01
CN117043331A (zh) 2023-11-10
KR20230158660A (ko) 2023-11-21
JP2024512050A (ja) 2024-03-18

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