CL2023002310A1 - Un mutante de las transcetolasas r con alta estereoselectividad y su gen codificante y aplicación - Google Patents
Un mutante de las transcetolasas r con alta estereoselectividad y su gen codificante y aplicaciónInfo
- Publication number
- CL2023002310A1 CL2023002310A1 CL2023002310A CL2023002310A CL2023002310A1 CL 2023002310 A1 CL2023002310 A1 CL 2023002310A1 CL 2023002310 A CL2023002310 A CL 2023002310A CL 2023002310 A CL2023002310 A CL 2023002310A CL 2023002310 A1 CL2023002310 A1 CL 2023002310A1
- Authority
- CL
- Chile
- Prior art keywords
- mutant
- transketolase
- application
- high stereoselectivity
- stereoselectivity
- Prior art date
Links
- 102000014701 Transketolase Human genes 0.000 title abstract 6
- 108010043652 Transketolase Proteins 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 150000003934 aromatic aldehydes Chemical class 0.000 abstract 2
- 125000003118 aryl group Chemical group 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 238000003259 recombinant expression Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000006555 catalytic reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01001—Transketolase (2.2.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se proporciona un mutante de transcetolasa, su gen codificante y aplicación, se refiere al campo técnico de la ingeniería enzimática. El mutante de transcetolasa proporcionada tiene la secuencia que se muestra en SEQ ID NO:16, es una secuencia en que se produce la mutación de aminoácidos. Se proporciona un mutante de la transcetolasa R con alta estereoselectividad, cuya secuencia de aminoácidos es como se muestra en SEQ ID NO:1; el vector de expresión recombinante que contiene el mencionado gen codificante; la célula huésped que contiene el mencionado vector de expresión recombinante; un método para preparar el mutante de la transcetolasa R con alta estereoselectividad; la aplicación del mencionado mutante de la transcetolasa R al formar la R-dihidroxicetona aromática con alta estereoselectividad a través de catalizar el aldehído aromático; la aplicación de su gen codificante al formar la R-dihidroxicetona aromática con alta estereoselectividad a través de catalizar el aldehído aromático. La estereoselectividad de la p-tiamfenilfenildihidroxicetona producida por el mutante EcTK1_YYH mediante la catálisis es del 95,2% de ee(R), con una alta actividad y una alta estereoselectividad.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110162619.2A CN114875004B (zh) | 2021-02-05 | 2021-02-05 | 一种高立体选择性r转酮酶突变体及其编码基因和应用 |
| CN202110567238 | 2021-05-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2023002310A1 true CL2023002310A1 (es) | 2024-04-26 |
Family
ID=82740923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2023002310A CL2023002310A1 (es) | 2021-02-05 | 2023-08-04 | Un mutante de las transcetolasas r con alta estereoselectividad y su gen codificante y aplicación |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4289947A1 (es) |
| KR (1) | KR20230137996A (es) |
| CN (1) | CN117157395A (es) |
| BR (1) | BR112023015718A2 (es) |
| CL (1) | CL2023002310A1 (es) |
| MX (1) | MX2023009214A (es) |
| WO (1) | WO2022166843A1 (es) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10032254B4 (de) * | 2000-07-03 | 2006-05-04 | Forschungszentrum Jülich GmbH | Nukleotidsequenz kodierend für eine Benzaldehyd-Lyase und Verfahren zur stereoselektiven Synthese von (R)-2-Hydroxyketonen |
| EP1957640B1 (en) * | 2005-11-02 | 2013-05-29 | DSM IP Assets B.V. | Modified transketolase and use thereof |
| KR102223521B1 (ko) * | 2019-02-26 | 2021-03-05 | 한국과학기술원 | 안트라닐산 메틸 생성능을 가지는 재조합 미생물 및 이를 이용한 안트라닐산 메틸의 제조방법 |
-
2022
- 2022-01-28 BR BR112023015718A patent/BR112023015718A2/pt unknown
- 2022-01-28 EP EP22749122.2A patent/EP4289947A1/en active Pending
- 2022-01-28 KR KR1020237029610A patent/KR20230137996A/ko unknown
- 2022-01-28 WO PCT/CN2022/074751 patent/WO2022166843A1/zh active Application Filing
- 2022-01-28 MX MX2023009214A patent/MX2023009214A/es unknown
- 2022-01-28 CN CN202280019980.2A patent/CN117157395A/zh active Pending
-
2023
- 2023-08-04 CL CL2023002310A patent/CL2023002310A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022166843A1 (zh) | 2022-08-11 |
| BR112023015718A2 (pt) | 2023-11-07 |
| EP4289947A1 (en) | 2023-12-13 |
| MX2023009214A (es) | 2023-08-22 |
| KR20230137996A (ko) | 2023-10-05 |
| CN117157395A (zh) | 2023-12-01 |
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