CL2019001378A1 - Microcompartimento celular y métodos de preparación. - Google Patents

Microcompartimento celular y métodos de preparación.

Info

Publication number
CL2019001378A1
CL2019001378A1 CL2019001378A CL2019001378A CL2019001378A1 CL 2019001378 A1 CL2019001378 A1 CL 2019001378A1 CL 2019001378 A CL2019001378 A CL 2019001378A CL 2019001378 A CL2019001378 A CL 2019001378A CL 2019001378 A1 CL2019001378 A1 CL 2019001378A1
Authority
CL
Chile
Prior art keywords
preparation methods
layer
cell microcompartment
microcompartment
cell
Prior art date
Application number
CL2019001378A
Other languages
English (en)
Inventor
Maxime Feyeux
Kevin Alessandri
Pierre Nassoy
Laurent Cognet
Gaëlle Recher
Erwan Bezard
Original Assignee
Univ Bordeaux
Centre Nat Rech Scient
Inst Doptique Theorique Et Appliquee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bordeaux, Centre Nat Rech Scient, Inst Doptique Theorique Et Appliquee filed Critical Univ Bordeaux
Publication of CL2019001378A1 publication Critical patent/CL2019001378A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Clinical Laboratory Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

LA INVENCIÓN SE REFIERE A UN MICROCOMPARTIMENTO CELULAR QUE COMPRENDE DE FORMA SUCESIVA, ORGANIZADAS ALREDEDOR DE UN LUMEN, AL MENOS UNA CAPA DE CÉLULAS PLURIPOTENTES, UNA CAPA DE MATRIZ EXTRACELULAR Y UNA CAPA EXTERNA DE HIDROGEL. LA INVENCIÓN TAMBIÉN SE REFIERE A MÉTODOS DE PREPARACIÓN DE MICROCOMPARTIMENTOS CELULARES DE ESE TIPO.
CL2019001378A 2016-11-23 2019-05-22 Microcompartimento celular y métodos de preparación. CL2019001378A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1661377A FR3059009B1 (fr) 2016-11-23 2016-11-23 Microcompartiment cellulaire et procedes de preparation

Publications (1)

Publication Number Publication Date
CL2019001378A1 true CL2019001378A1 (es) 2020-01-10

Family

ID=58992916

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2019001378A CL2019001378A1 (es) 2016-11-23 2019-05-22 Microcompartimento celular y métodos de preparación.

Country Status (13)

Country Link
US (3) US11807870B2 (es)
EP (2) EP3545080A1 (es)
JP (2) JP7154525B2 (es)
KR (3) KR20240091112A (es)
CN (1) CN110603319A (es)
AU (2) AU2017365846B2 (es)
BR (1) BR112019010512A2 (es)
CA (1) CA3040781A1 (es)
CL (1) CL2019001378A1 (es)
FR (1) FR3059009B1 (es)
IL (1) IL266777B2 (es)
MX (2) MX2019006050A (es)
WO (1) WO2018096277A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021018174A2 (pt) * 2019-03-13 2021-11-16 Membrane Protective Tech Inc Métodos e sistemas para a suplementação protetora durante depressão de temperatura.
FR3098223A1 (fr) 2019-07-05 2021-01-08 Universite de Bordeaux Procede de production in vitro de neurones de mammiferes
FR3099882A1 (fr) * 2019-08-12 2021-02-19 Treefrog Therapeutics Unité tridimensionnelle creuse de tissu rétinien et utilisation dans le traitement des rétinopathies
FR3108623A1 (fr) * 2020-03-27 2021-10-01 Treefrog Therapeutics Procédé de caractérisation d’un micro-tissu biologique par imagerie
FR3114322A1 (fr) 2020-09-21 2022-03-25 Treefrog Therapeutics Tissu cardiaque particulier et utilisation dans le traitement de pathologies cardiaque
FR3114321A1 (fr) 2020-09-21 2022-03-25 Treefrog Therapeutics Microcompartiments cellulaires comprenant des cellules humaines en cours de différenciation cardiaque, tissus obtenus à partir de ces microcompartiments et utilisations
IL309237A (en) * 2021-06-16 2024-02-01 Treefrog Therapeutics Large cellular microclasses that include a large number of cysts
FR3124193B3 (fr) * 2021-06-16 2023-09-22 Treefrog Therapeutics Microcompartiments cellulaires de grande taille comprenant plusieurs cystes
WO2023235884A1 (en) 2022-06-03 2023-12-07 Flagship Pioneering Innovations Vi, Llc Compositions and methods
FR3138661A1 (fr) 2022-08-08 2024-02-09 Treefrog Therapeutics Substitut de matrice extracellulaire dans un microcompartiment cellulaire
WO2024033284A1 (fr) 2022-08-08 2024-02-15 Treefrog Therapeutics Substitut de matrice extracellulaire dans un microcompartiment cellulaire
FR3141945A1 (fr) 2022-11-15 2024-05-17 Treefrog Therapeutics Substitut de matrice extracellulaire dans un microcompartiment cellulaire

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9150829B2 (en) * 2009-03-20 2015-10-06 Agency For Science, Technoloy And Research Culture of pluripotent and multipotent cells on microcarriers
US9487756B2 (en) * 2009-03-20 2016-11-08 Mesoblast, Inc. Production of reprogrammed pluripotent cells
JP5365863B2 (ja) * 2009-08-21 2013-12-11 株式会社リコー トナーの製造装置及びトナーの製造方法
JP5633077B2 (ja) * 2009-10-14 2014-12-03 国立大学法人 東京大学 被覆されたマイクロゲルファイバ
FR2986165B1 (fr) * 2012-01-31 2015-07-24 Capsum Procede de preparation de capsules rigidifiees
US20140127290A1 (en) * 2012-11-08 2014-05-08 Ohio State Innovation Foundation Microcapsules Encapsulating Living Cells
WO2014176697A1 (en) * 2013-04-30 2014-11-06 Chen haotian Microfluidic devices and methods for the extrusion of tubular structures
US9587221B2 (en) * 2013-11-11 2017-03-07 Auburn University Encapsulation and cardiac differentiation of hiPSCs in 3D PEG-fibrinogen hydrogels
EP3147346A4 (en) * 2014-05-20 2018-01-10 The University of Tokyo Hollow microfiber
AU2016226178B2 (en) * 2015-03-03 2021-07-29 President And Fellows Of Harvard College Methods of generating functional human tissue
WO2016182022A1 (ja) * 2015-05-14 2016-11-17 公立大学法人横浜市立大学 高分子を細胞と共に凝集させる技術
FR3058892B1 (fr) 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere

Also Published As

Publication number Publication date
MX2023008382A (es) 2023-07-31
WO2018096277A1 (fr) 2018-05-31
FR3059009B1 (fr) 2018-12-07
FR3059009A1 (fr) 2018-05-25
US20240018469A1 (en) 2024-01-18
JP2022166041A (ja) 2022-11-01
AU2024203214A1 (en) 2024-05-30
AU2017365846B2 (en) 2024-03-28
KR20190111013A (ko) 2019-10-01
JP2019535326A (ja) 2019-12-12
US20230117999A1 (en) 2023-04-20
CA3040781A1 (fr) 2018-05-31
KR102671098B1 (ko) 2024-05-30
US11807870B2 (en) 2023-11-07
JP7154525B2 (ja) 2022-10-18
IL266777B1 (en) 2024-05-01
KR20230169414A (ko) 2023-12-15
IL266777A (en) 2019-07-31
EP4223870A1 (fr) 2023-08-09
IL266777B2 (en) 2024-09-01
US20190330589A1 (en) 2019-10-31
MX2019006050A (es) 2019-12-09
CN110603319A (zh) 2019-12-20
BR112019010512A2 (pt) 2019-09-17
KR20240091112A (ko) 2024-06-21
AU2017365846A1 (en) 2019-05-16
EP3545080A1 (fr) 2019-10-02

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