CL2017002897A1 - Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas. - Google Patents

Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas.

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Publication number
CL2017002897A1
CL2017002897A1 CL2017002897A CL2017002897A CL2017002897A1 CL 2017002897 A1 CL2017002897 A1 CL 2017002897A1 CL 2017002897 A CL2017002897 A CL 2017002897A CL 2017002897 A CL2017002897 A CL 2017002897A CL 2017002897 A1 CL2017002897 A1 CL 2017002897A1
Authority
CL
Chile
Prior art keywords
ifng
transformed
food
composition
immunostimulate
Prior art date
Application number
CL2017002897A
Other languages
English (en)
Inventor
Cesar Gerardo Tello Reyes Mario
Eugenio Santibañez Varga Alvaro
Philippe Parra Mardonez Mick
Enrique Paine Cabrera Diego
Andrea Zapata Rojas Claudia
Del Pilar Garces Fernandez Andrea
Original Assignee
Univ De Santiago De Chile 10%
Consorcio Tecnologico De Sanidad Acuicola S A 90%
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ De Santiago De Chile 10%, Consorcio Tecnologico De Sanidad Acuicola S A 90% filed Critical Univ De Santiago De Chile 10%
Priority to CL2017002897A priority Critical patent/CL2017002897A1/es
Priority to US16/764,356 priority patent/US20220347235A1/en
Priority to EP18878202.3A priority patent/EP3750984A4/en
Priority to PCT/CL2018/050021 priority patent/WO2019095082A1/es
Publication of CL2017002897A1 publication Critical patent/CL2017002897A1/es
Priority to US18/467,280 priority patent/US20240131088A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • A61K38/217—IFN-gamma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/21—Streptococcus, lactococcus
    • A23V2400/231—Lactis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Insects & Arthropods (AREA)
  • Immunology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Plant Pathology (AREA)
  • Birds (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Nutrition Science (AREA)

Abstract

<p>Bacteria Lactococcus lactis transformada, productora de interferón gamma (IFNg) de Salmo salar que comprende la construcción de ADN pNZ8149-P1-USP45-IFNg-GGG-6xHIS, donde pNZ8149 es el vector transformado; P1, el promotor de expresión constitutiva de L. lactis; Usp45 es la señal de exportación; IFNg es la secuencia madura de mRNA codificante para IFN gamma de Salmo salar; GGG es una secuencia que codifica para tres glicinas y 6xHIS es una secuencia que codifica para 6 Histidinas terminales. Es motivo de la invención el plásmido que contiene la construcción pNZ8149-P1-USP45-IFNg-GGG-6xHIS, el alimento y composición que comprende la bacteria L. lactis transformada. Así mismo, el invento se relaciona con el uso de la bacteria transformada con IFNg, para preparar un alimento o composición para inmunoestimular especies acuícolas.</p>
CL2017002897A 2017-11-14 2017-11-14 Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas. CL2017002897A1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CL2017002897A CL2017002897A1 (es) 2017-11-14 2017-11-14 Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas.
US16/764,356 US20220347235A1 (en) 2017-11-14 2018-04-20 Transformed, salmo salar interferon gamma (ifng)-producing lactococcus lactis bacterium, food and composition comprising same, for immunostimulation in aquaculture species
EP18878202.3A EP3750984A4 (en) 2017-11-14 2018-04-20 TRANSFORMED INTERFERON GAMMA (IFNG) PRODUCING LACTOCOCCUS LACTIS FROM SALMO SALAR, FOOD AND COMPOSITION FOR IMMUNESTIMULATION IN AQUACULTURE SPECIES
PCT/CL2018/050021 WO2019095082A1 (es) 2017-11-14 2018-04-20 Bacteria lactococcus lactis transformada, productora de interferon gamma (ifng) de salmo salar, alimento y composicion que la comprende, para inmunoestimular especies acuicolas
US18/467,280 US20240131088A1 (en) 2017-11-14 2023-09-14 Method for preparing a transformed, salmo salar interferon gamma (ifng)-producing lactococcus lactis bacterium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2017002897A CL2017002897A1 (es) 2017-11-14 2017-11-14 Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas.

Publications (1)

Publication Number Publication Date
CL2017002897A1 true CL2017002897A1 (es) 2018-04-20

Family

ID=63046412

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2017002897A CL2017002897A1 (es) 2017-11-14 2017-11-14 Bacteria lactococcus lactis transformada, productora de interferón gamma (ifng) de salmo salar, alimento y composición que la comprende, para inmunoestimular especies acuícolas.

Country Status (4)

Country Link
US (2) US20220347235A1 (es)
EP (1) EP3750984A4 (es)
CL (1) CL2017002897A1 (es)
WO (1) WO2019095082A1 (es)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4808523A (en) 1984-11-07 1989-02-28 Yeda Research And Development Co., Ltd. Constitutive production of human IFN-β1 by mammalian cells transformed by the IFN-β1 gene fused to an SV40 early promoter
JP2006265181A (ja) * 2005-03-24 2006-10-05 Univ Of Tsukuba 魚類感染症予防製剤、魚類用餌、菌株、魚類感染症予防方法
WO2010139195A1 (en) 2009-06-04 2010-12-09 The Chinese University Of Hong Kong Anti-inflammatory bacteria
CN201503797U (zh) 2009-09-15 2010-06-09 河南森源电气股份有限公司 一种隔离开关转轴与拐臂连接结构
WO2011150127A2 (en) 2010-05-25 2011-12-01 The Board Of Trustees Of The University Of Illinois Novel systems, vectors, and methods for delivery of biomolecules to eukaryotic cells
CN102329766A (zh) 2011-09-16 2012-01-25 暨南大学 可用于预防或治疗人溃疡性结肠炎的重组食品级乳酸菌、其制备方法与应用
CN102796755B (zh) 2012-06-28 2015-02-04 郑州大学 一种乳酸乳球菌表达载体及其制备方法与应用
WO2014040987A1 (en) * 2012-09-11 2014-03-20 University of Tromsø The use of a nucleic acid sequence encoding a type i interferon (ifn) originating from atlantic salmon as an antiviral and immune stimulating agent
CN103074291A (zh) 2012-12-20 2013-05-01 大连大学 一种重组猪流行性腹泻病毒乳酸乳球菌、其构建方法及应用
WO2014118385A1 (en) * 2013-02-04 2014-08-07 University of Tromsø The use of dna sequences encoding an interferon as vaccine adjuvants
CN104120142B (zh) 2014-06-30 2016-06-01 华南农业大学 一种分泌表达pedv核心抗原coe蛋白重组乳酸乳球菌及其制备方法和应用
CL2014002394A1 (es) * 2014-09-10 2014-11-21 Univ Chile Método para producir probioticos autóctonos con actividad inmunoestimulaste y sus uso en profilaxis contra flavobacteriosis en salmónidos
CN105331570B (zh) 2015-11-30 2019-04-02 中国科学院水生生物研究所 一种含有鲫鱼ifn干扰素的蓝藻工程菌及应用

Also Published As

Publication number Publication date
WO2019095082A1 (es) 2019-05-23
US20240131088A1 (en) 2024-04-25
US20220347235A1 (en) 2022-11-03
EP3750984A4 (en) 2021-10-27
EP3750984A1 (en) 2020-12-16

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