CL2009000193A1 - Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. - Google Patents

Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.

Info

Publication number
CL2009000193A1
CL2009000193A1 CL2009000193A CL2009000193A CL2009000193A1 CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1 CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1
Authority
CL
Chile
Prior art keywords
vector
plant cell
genes
virus
cell
Prior art date
Application number
CL2009000193A
Other languages
English (en)
Inventor
Valerian V Dolja
Valera V Peremyslov
Original Assignee
The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St filed Critical The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon St
Publication of CL2009000193A1 publication Critical patent/CL2009000193A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

UN VECTOR PARA LA EXPRESION DE GENES CAPAZ DE REPLICARSE EN VIDES QUE COMPRENDE UN ÁCIDO NUCLEICO QUE CODIFICA: A) GENES VIRALES DEL GRAPEVINE LEAFROL-ASSOCIATED VIRUS-2 (LR-2) COMPRENDIENDO UNA METILTRANSFERASA, UNA ARN HELICASA, Y UNA ARN POLMERASA DEPENDIENTE DE ARN; B) UN ARNI SUPRESOR SELECCIONADO DE) GRUPO FORMADO POR P24 DE LR-2 Y P21 DEL VIRUS AMARILLO DE LA REMOLACHA; C) PROTEASAS DIRECTRICES L1 Y L2 DE LR-2; Y D) UN POLINUCLEÁTIDO HETERÓLOGO UNIDO OPERATIVAMENTE A UN PROMOTOR. UNA CÉLULA DE VID QUE COMPRENDE DICHO VECTOR. UN MÉTODO PARA EXPRESAR UN GEN HETERÓLOGO EN UNA CÉLULA DE VID QUE COMPRENDE INTRODUCIR DICHO VECTOR EN LA CÉLULA DE VID. UN MÉTODO PARA PRODUCIR DICHO VECTOR QUE ADEMÁS COMPRENDE RECUPERAR E) VECTOR DE A CÉLULA DE VID O DEL MEDIO DONDE SE CULTIVA LA CÉLULA DE VID.
CL2009000193A 2008-01-31 2009-01-29 Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. CL2009000193A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6330508P 2008-01-31 2008-01-31
US8350408P 2008-07-24 2008-07-24

Publications (1)

Publication Number Publication Date
CL2009000193A1 true CL2009000193A1 (es) 2009-08-21

Family

ID=40952630

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2009000193A CL2009000193A1 (es) 2008-01-31 2009-01-29 Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.

Country Status (9)

Country Link
US (2) US8415147B2 (es)
EP (1) EP2240580B1 (es)
CN (1) CN101978051A (es)
AR (1) AR070326A1 (es)
AU (1) AU2009210508B2 (es)
CL (1) CL2009000193A1 (es)
ES (1) ES2478041T3 (es)
WO (1) WO2009099877A2 (es)
ZA (1) ZA201005866B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102933713B (zh) * 2010-04-08 2015-05-13 佛罗里达大学研究基金会有限公司 具有多功能的瞬时表达病毒载体体系
US8389804B2 (en) 2010-04-08 2013-03-05 University Of Florida Research Foundation, Inc. Viral based transient-expression vector system that allows multiple applications
WO2012048221A1 (en) * 2010-10-08 2012-04-12 Fraunhofer Usa Inc. Closterovirus-based nucleic acid molecules and uses thereof
CN105939599B (zh) 2011-09-21 2019-10-18 佛罗里达大学研究基金会有限公司 使用柑橘衰退病毒载体进行外源基因表达
US10017747B2 (en) 2011-09-21 2018-07-10 University Of Florida Research Foundation, Inc. Citrus tristeza virus based vectors for foreign gene/s expression
CN103421839B (zh) * 2013-07-24 2015-04-29 河南农业大学 瓜类褪绿黄化病毒侵染性载体的构建方法
CN105063038B (zh) * 2015-09-01 2017-12-26 中华人民共和国伊犁出入境检验检疫局 一种检测葡萄卷叶伴随病毒3号的rpa引物及试剂盒
CN109777792B (zh) * 2018-09-29 2020-12-11 中国科学院动物研究所 一种rna解旋酶3及其编码基因和应用
CN110144338A (zh) * 2019-05-29 2019-08-20 通用生物系统（安徽）有限公司 一种以rna为模板的rna聚合酶制备方法及该聚合酶的应用
CN117479837A (zh) * 2021-01-21 2024-01-30 耶路撒冷希伯来大学伊萨姆研发有限公司 RNAi纳米颗粒及其在农业中的使用方法
CN117305267B (zh) * 2023-08-09 2024-05-28 中国农业大学 葡萄类受体蛋白激酶herk1的新用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6093544A (en) 1997-05-20 2000-07-25 Cornell Research Foundation, Inc. Rupestris stem pitting associated virus nucleic acids, proteins, and their uses
US6197948B1 (en) * 1997-05-20 2001-03-06 Cornell Research Foundation, Inc. Grapevine leafroll virus (type 2) proteins and their uses
US20020104126A1 (en) 2000-10-18 2002-08-01 Pogue Gregory P. Production of bovine lysozyme by plant viral vectors
DE10109354A1 (de) 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
US20060228800A1 (en) * 2003-05-15 2006-10-12 Shi-Lung Lin Novel Transgenic Methods Using intronic RNA

Also Published As

Publication number Publication date
EP2240580B1 (en) 2014-04-30
US8415147B2 (en) 2013-04-09
US20130133109A1 (en) 2013-05-23
AU2009210508A1 (en) 2009-08-13
ZA201005866B (en) 2011-11-30
CN101978051A (zh) 2011-02-16
AR070326A1 (es) 2010-03-31
EP2240580A2 (en) 2010-10-20
AU2009210508B2 (en) 2015-04-09
US20100333230A1 (en) 2010-12-30
WO2009099877A3 (en) 2010-01-07
ES2478041T3 (es) 2014-07-18
WO2009099877A2 (en) 2009-08-13
EP2240580A4 (en) 2011-09-07

Similar Documents

Publication Publication Date Title
CL2009000193A1 (es) Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.
AR110075A1 (es) Un método para edición basal en plantas
AR114014A1 (es) Sistema de edición de bases y método basado en proteína cpf1
EA201690374A1 (ru) Растения с повышенной устойчивостью к гербицидам
NZ616646A (en) Synthetic genes
AR054171A1 (es) Aumento de rendimiento en plantas con sobreexpresion de los genes accdp
IN2014MN02498A (es)
Taliansky et al. RNA-based technologies for engineering plant virus resistance
MX2009012451A (es) Plantas con rasgos mejorados relacionados con el rendimiento y un metodo para producirlas.
MX2012002045A (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
MX2009007246A (es) Plantas con rasgos relacionados con el rendimiento mejorado y un metodo para producirlas.
AR076473A1 (es) Manipulacion de la biosintesis de fructano y aumento de biomasa en plantas
BR112012017896A2 (pt) Excisão de transgenes em organismos geneticamente modificados
MX2022015112A (es) Inductor haploide.
MX2012003451A (es) Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para prepararlas.
EA201290168A1 (ru) Растения, обладающие повышенными урожайностными свойствами, и способ создания таких растений
MX2013014712A (es) Produccion de particulas tipo virus de la rabia en plantas.
EA201490715A1 (ru) Рнк-интерференция для борьбы с грибами и оомицетами путем ингибирования гена сахаропиндегидрогеназы
EA201170752A1 (ru) Растения с повышенной устойчивостью к абиотическому стрессу и/или улучшенными характеристиками урожайности и способ их получения
EA201290936A1 (ru) Растения с улучшенными характеристиками урожайности и способ их получения
AR092291A1 (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas
AR120136A1 (es) Plantas modificadas que comprende una mutación en una secuencia de ácido nucleico lazy4
EA201200468A1 (ru) Ингибирование стрелкования и цветения растений сахарной свеклы
AR103920A1 (es) Moléculas de ácido nucleico de ii215 de arn polimerasa para el control de plagas de insectos
AR113336A1 (es) Un método para incrementar el rendimiento de un cultivo que comprende transformar una planta con por lo menos una secuencia que codifica la proteína ferredoxina-tiorredoxina reductasa, constructo y planta