CL2003001698A1 - Complejo promotor bi-direccional que comprende una region amplificadora modificada con al menos dos regiones amplificadoras y por lo menos dos promotores basicos; vector que comprende dicho complejo; celular transfectada con dicho vector y metodo par - Google Patents

Complejo promotor bi-direccional que comprende una region amplificadora modificada con al menos dos regiones amplificadoras y por lo menos dos promotores basicos; vector que comprende dicho complejo; celular transfectada con dicho vector y metodo par

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Publication number
CL2003001698A1
CL2003001698A1 CL200301698A CL2003001698A CL2003001698A1 CL 2003001698 A1 CL2003001698 A1 CL 2003001698A1 CL 200301698 A CL200301698 A CL 200301698A CL 2003001698 A CL2003001698 A CL 2003001698A CL 2003001698 A1 CL2003001698 A1 CL 2003001698A1
Authority
CL
Chile
Prior art keywords
vector
complex
amplifier
transfected cell
understanding
Prior art date
Application number
CL200301698A
Other languages
English (en)
Inventor
Zhijian Y Dennis J Gray Li
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of CL2003001698A1 publication Critical patent/CL2003001698A1/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CL200301698A 2001-02-13 2003-08-22 Complejo promotor bi-direccional que comprende una region amplificadora modificada con al menos dos regiones amplificadoras y por lo menos dos promotores basicos; vector que comprende dicho complejo; celular transfectada con dicho vector y metodo par CL2003001698A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US26835801P 2001-02-13 2001-02-13

Publications (1)

Publication Number Publication Date
CL2003001698A1 true CL2003001698A1 (es) 2005-01-28

Family

ID=23022621

Family Applications (1)

Application Number Title Priority Date Filing Date
CL200301698A CL2003001698A1 (es) 2001-02-13 2003-08-22 Complejo promotor bi-direccional que comprende una region amplificadora modificada con al menos dos regiones amplificadoras y por lo menos dos promotores basicos; vector que comprende dicho complejo; celular transfectada con dicho vector y metodo par

Country Status (6)

Country Link
US (1) US7129343B2 (es)
EP (1) EP1360310A2 (es)
AU (1) AU2002250064B2 (es)
CA (1) CA2443266A1 (es)
CL (1) CL2003001698A1 (es)
WO (1) WO2002064804A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2848570B1 (fr) 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
WO2005019459A1 (de) 2003-07-22 2005-03-03 Sungene Gmbh & Co. Kgaa Expressionskassetten zur bidirektionalen transgenen expression von nukleinsäuren in pflanzen
CN1936002A (zh) * 2005-09-19 2007-03-28 中国农业大学 一种分离双向启动子的方法及其应用
US20070283455A1 (en) * 2006-05-31 2007-12-06 Gray Dennis J Genetic Transformation of Grapevines
US9150877B2 (en) 2007-09-27 2015-10-06 Dow Agrosciences Llc Construct and method for expressing transgenes using a Brassica bidirectional constitutive promoter
BR112014014029B1 (pt) 2011-12-30 2021-12-14 Dow Agrosciences Llc Polinucleotídeo compreendendo um promotor bidirecional
JP6220794B2 (ja) 2011-12-30 2017-10-25 ダウ アグロサイエンシィズ エルエルシー 合成二方向植物プロモーターubi1のための構築物および方法
CN112472814A (zh) 2012-01-23 2021-03-12 萨奇治疗股份有限公司 神经活性类固醇制剂和治疗中枢神经系统障碍的方法
CN104540951B (zh) 2012-06-07 2017-07-04 美国陶氏益农公司 使用芸苔属双向组成型启动子表达转基因的构建体和方法
EP2912178B1 (en) 2012-10-25 2020-06-17 Whitehead Institute For Biomedical Research Super-enhancers and methods of use thereof
US20140296218A1 (en) 2012-10-25 2014-10-02 Whitehead Institute For Biomedical Research Super-enhancers and methods of use thereof
US10160977B2 (en) 2012-10-25 2018-12-25 Whitehead Institute For Biomedical Research Super-enhancers and methods of use thereof
US11319591B2 (en) 2014-03-19 2022-05-03 Whitehead Institute For Biomedical Research Core transcriptional circuitry in human cells and methods of use thereof
TW201619386A (zh) * 2014-11-11 2016-06-01 陶氏農業科學公司 合成性雙向植物啓動子
BR102015026078A2 (pt) * 2015-10-14 2017-05-16 Inst Agronômico Do Paraná - Iapar Method for production of transgenic plants and plants with resistance to bacteria that have the quorum sensing system
CN109414444A (zh) 2016-03-08 2019-03-01 萨奇治疗股份有限公司 神经活性类固醇、其组合物及用途
US20210015898A1 (en) * 2018-04-09 2021-01-21 Allen Institute Rescuing voltage-gated sodium channel function in inhibitory neurons

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AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
US5359142A (en) * 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
US4966841A (en) * 1987-05-22 1990-10-30 The Board Of Regents Of The University Of Washington Enhanced vector production and expression of recombinant DNA products
US5876962A (en) * 1987-08-12 1999-03-02 Natural Environment Research Council Expression vectors for the synthesis of proteins and plasmid replicons and sequence cassettes for use in constructing such vectors
EP0414689B1 (en) * 1988-02-12 1994-12-07 Commonwealth Scientific And Industrial Research Organisation Pox virus vectors
US6172039B1 (en) * 1990-04-16 2001-01-09 Apex Bioscience, Inc. Expression of recombinant hemoglobin and hemoglobin variants in yeast
US5633446A (en) * 1990-04-18 1997-05-27 Plant Genetic Systems, N.V. Modified Bacillus thuringiensis insecticidal-crystal protein genes and their expression in plant cells
FR2680518A1 (fr) * 1991-08-21 1993-02-26 Rhone Poulenc Rorer Sa Promoteur de levure et son utilisation.
US6008051A (en) * 1992-09-14 1999-12-28 University Of Massachusetts Recombinant vector and process for cell flotation
US6004941A (en) * 1993-06-14 1999-12-21 Basf Aktiengesellschaft Methods for regulating gene expression
US5912411A (en) * 1993-06-14 1999-06-15 University Of Heidelberg Mice transgenic for a tetracycline-inducible transcriptional activator
US5866755A (en) * 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
US5547862A (en) * 1993-07-29 1996-08-20 Ambion Inc. Vectors containing multiple promoters in the same orientation
CA2174954C (en) * 1993-11-19 2005-03-15 Stanton B. Gelvin Chimeric regulatory regions and gene cassettes for expression of genes in plants
ATE243745T1 (de) * 1994-01-31 2003-07-15 Univ Boston Bibliotheken aus polyklonalen antikörpern
US5693508A (en) * 1994-11-08 1997-12-02 Chang; Lung-Ji Retroviral expression vectors containing MoMLV/CMV-IE/HIV-TAR chimeric long terminal repeats
US5691140A (en) * 1995-05-18 1997-11-25 New England Biolabs, Inc. Bidirectional in vitro transcription vectors utilizing a single RNA polymerase for both directions
US5891718A (en) * 1996-03-27 1999-04-06 Vical Incorporated Tetracycline inducible/repressible systems
US6110668A (en) * 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
HUP0001650A3 (en) * 1996-11-08 2001-09-28 Neose Technologies Inc Horsham Improved expression vectors
FR2757061B1 (fr) * 1996-12-16 1999-03-26 Rhone Merieux Vaccin vivant recombinant aviaire, utilisant comme vecteur le virus de la laryngotracheite infectieuse aviaire
US5990091A (en) * 1997-03-12 1999-11-23 Virogenetics Corporation Vectors having enhanced expression, and methods of making and uses thereof
US6004777A (en) * 1997-03-12 1999-12-21 Virogenetics Corporation Vectors having enhanced expression, and methods of making and uses thereof
US5968773A (en) * 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
US6388170B1 (en) * 2000-04-07 2002-05-14 University Of Kentucky Research Foundation Bidirectional promoters and methods related thereto

Also Published As

Publication number Publication date
WO2002064804A3 (en) 2003-04-17
US20050188432A1 (en) 2005-08-25
EP1360310A2 (en) 2003-11-12
US7129343B2 (en) 2006-10-31
WO2002064804A2 (en) 2002-08-22
AU2002250064B2 (en) 2008-01-17
CA2443266A1 (en) 2002-08-22

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